A device for bilateral imaging of living animals and its use method

Abstract

The present invention discloses a bilateral imaging device for a living animal and a method for using the device, which relates to the field of biotechnology and includes an imaging structure and a rotating platform. The imaging structure includes an imaging laser and a lens for receiving light signals. The imaging lasers are symmetrically arranged on both sides of the central axis of the lens. The longitudinal axis of the living animal and the central axis are located in the same plane, and the plane where the longitudinal axis is located is perpendicular to the plane where the imaging lasers are located. The living animal is placed on the rotating platform. The present invention controls the rotation angle of the rotating platform to achieve the imaging laser being emitted into the imaging parts on both sides of the living animal, which can ensure the consistency of the selection of the imaging parts. The time interval between imaging the imaging parts on both sides is shortened as much as possible through the operation of the rotating platform, thereby maximizing the accuracy of the imaging results in the comparative experiment. At the same time, the imaging results of the bilaterally symmetrical imaging parts are obtained by receiving the light signal through a single lens, which can reduce the cost of the experimental equipment.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to a device for bilateral imaging of living animals and a method for using the same. Background Art

[0002] In 1999, Weiss-Leder et al. at Harvard University proposed the concept of molecular imaging—the application of imaging methods to qualitatively and quantitatively investigate biological processes in vivo at the cellular and molecular levels. Currently, many mature instruments are available for in vivo imaging of small animals, but these imaging platforms often require the animals to be anesthetized with gases or immobilized in a suspended manner. Anesthesia with gases such as isoflurane maintains the animal's position during imaging, while the rotation of the fixture changes the animal's orientation, enabling imaging in any orientation. However, in studies involving the effects of gravity on small animal body fluids, the suspended immobilization method cannot image the animal in its natural prone position, resulting in experimental results that are inconsistent with real-world conditions. Furthermore, existing imaging platforms cannot simultaneously image both eyes of animals in the prone position. Furthermore, due to spatial limitations, quantitative automatic rotation and automatic adjustment of the excitation laser angle are impossible, resulting in inconsistent results in experiments requiring comparison of bilateral imaging results, such as binocular comparison.

[0003] In addition, there is a machine for imaging small living animals that can fill the entire space with laser light and then take an image through a lens on top of the animal's head. However, this imaging method is not very effective because the laser intensity is too weak, and the imaging effect on specific parts such as the optic nerve behind the eyeball is limited, making it almost impossible to image fluorescent particles.

[0004] A Chinese patent with authorization publication number CN215130748U discloses an adjustable fixed platform for a small animal live imaging device. The device comprises a drive motor, a rotating shaft, an adjustment plate 1, a linear motor, and an adjustment plate 2. The output shaft of the drive motor drives the rotating shaft to rotate via a gear belt, thereby causing the adjustment plate 1 to move laterally. The linear motor installed on the adjustment plate 1 can drive the adjustment plate 2 to move longitudinally, thereby facilitating the change of the position of the animal above the support platform. The device comprises a fixed plate, a threaded column, a rotating plate, and a vertical plate, which can limit and fix the animal placed on the adjustment plate 2. However, the solution only involves a platform structure that can be adjusted longitudinally and transversely, and does not provide information on how the imaging device is used in conjunction with the longitudinally and transversely adjustable platform.

[0005] A Chinese patent with authorization publication number CN211797028U discloses an adjustable fixed platform for a small animal live imaging device. During use, the animal is removed from the anesthesia box and placed in the imaging device. The animal is placed on the adjustable fixed platform and its mouth is inserted into the anesthesia ventilation tube fixed to the platform. The vertical, horizontal, and leftward positions of the mice on the experimental platform are adjusted by adjusting the sliding relationship between the table top, the first adjustment panel, and the experimental table. The relevant scales are recorded, and the position is determined by the scales on each plane. Image acquisition is then performed. However, this solution does not specify how to use the imaging device in conjunction with the vertical, horizontal, and leftward adjustable platform. Summary of the Invention

[0006] The purpose of the present invention is to provide a bilateral imaging device for living animals and a method for using the same, so as to solve the problems existing in the above-mentioned prior art. In the present invention, by controlling the rotation angle of the rotating platform, the imaging laser is shot into the imaging parts on both sides of the living animal, which can ensure the consistency of the selection of the imaging parts. The time interval between imaging the imaging parts on both sides is shortened as much as possible through the operation of the rotating platform, thereby maximizing the accuracy of the imaging results in the comparative experiment. At the same time, the imaging results of the symmetrical imaging parts on both sides are obtained by receiving the light signal through a lens, which can reduce the cost of experimental equipment.

[0007] To achieve the above object, the present invention provides the following solutions:

[0008] The present invention provides a bilateral imaging device for a living animal, comprising an imaging structure and a rotating platform. The imaging structure comprises an imaging laser and a lens for receiving light signals. The imaging lasers are symmetrically arranged on both sides of the central axis of the lens. The longitudinal axis of the living animal and the central axis are located in the same plane, and the plane on which the longitudinal axis is located is perpendicular to the plane on which the imaging lasers are located. The living animal is placed on the rotating platform.

[0009] Preferably, it also includes an anesthesia structure for the living animal, which includes a hose for circulating anesthetic gas and an anesthesia machine for outputting the anesthetic gas, one end of the hose is connected to the anesthesia machine, and the other end of the hose is arranged directly above the nose of the living animal.

[0010] Preferably, the imaging structure also includes an optical path adjustment structure, which includes an emitter for emitting the imaging laser, a beam splitter for splitting the imaging laser, an optical path baffle for blocking the imaging laser on one side after the splitting, and a reflector for changing the path of the imaging laser.

[0011] Preferably, the emitter is coaxially arranged on the central axis toward the living animal, the beam splitter is arranged directly in front of the emitter, and the reflectors are symmetrically arranged on both sides of the central axis.

[0012] Preferably, the rotating platform includes a base and a rotating disk, the living animal is placed on the upper surface of the rotating disk, a plurality of vertical through holes are opened between the upper and lower surfaces of the rotating disk, and the rotating disk is rotatably connected to the base by inserting pins in the vertical through holes.

[0013] Preferably, the vertical through holes are uniformly opened in the circumferential direction along the radial direction of the rotating disk.

[0014] Preferably, it also includes a fixing structure for adjusting the posture of the living animal, the fixing structure includes a vertical rod and a horizontal rod, the bottom end of the vertical rod is plugged into the vertical through hole, and the top end of the vertical rod is connected to the horizontal rod.

[0015] Preferably, the bottom end of the vertical rod is provided with a limiting portion that moves axially along the vertical rod, the cross-sectional area of ​​the limiting portion is larger than the cross-sectional area of ​​the vertical through hole, and the top end of the vertical rod is provided with a horizontal through hole for the cross rod to pass through.

[0016] The present invention also provides a method for using a bilateral imaging device for a living animal, comprising the following steps:

[0017] Adjusting the optical path adjustment structure so that the imaging laser is symmetrically directed from both sides of the central axis of the lens to the same position on the central axis; placing the living animal on a rotating platform, adjusting the posture of the living animal using the fixed structure so that the longitudinal axis of the living animal and the central axis are located in the same plane, and the vertical plane of the longitudinal axis is perpendicular to the plane of the imaging laser; controlling the anesthesia structure to deliver anesthetic gas to the nose of the living animal, so that the nose is within the anesthetic range of the anesthetic gas;

[0018] The rotating platform is rotated from the initial state, and the imaging laser on the other side is blocked at the intersection of the imaging part of the living animal and the imaging laser on the same side, and the rotation angle of the rotating platform is recorded as A. The light signal reflected from the imaging part is received through the lens, and the initial state of the rotating platform is returned; the rotating platform is rotated in the opposite direction by an angle A from the initial state, and the imaging laser on the other side is blocked at the intersection of the imaging part and the imaging laser on the same side, and the light signal reflected from the imaging part is received through the lens, and the initial state of the rotating platform is returned.

[0019] Preferably, the imaging part is an eye on one side of a living animal, and the fixing structure adjusts the head and neck of the living animal toward the lens.

[0020] Compared with the prior art, the present invention has achieved the following technical effects:

[0021] (1) The present invention controls the rotation angle of the rotating platform to achieve the imaging laser shooting into the imaging parts on both sides of the living animal, which can ensure the consistency of the selection of the imaging parts. Compared with the prior art method of keeping the position of the living animal unchanged and using the adjustment instrument to change the light path, the imaging laser is kept stationary by rotating the living animal through the rotating platform, which can save the experimental operation space. At the same time, the time interval of imaging the imaging parts on both sides is shortened as much as possible through the operation of the rotating platform, so that the imaging results on both sides are as close as possible to the performance of the living animal in the same state, thereby maximizing the accuracy of the imaging results in the comparative experiment. At the same time, the imaging results of the symmetrical imaging parts on both sides are obtained by receiving the light signal through a lens, which can reduce the use cost of the experimental equipment.

[0022] (2) In the present invention, an optical path adjustment structure is provided, a beam splitter is used to split the imaging laser emitted by the laser, an optical path baffle is used to block any imaging laser after the splitting, and a reflector is combined to adjust the path of the imaging laser, and a rotating platform is used to rotate the living animal, so that laser irradiation imaging of the imaging parts symmetrical on both sides of the living animal can be achieved, and by blocking the imaging laser on the other side, interference of other light signals on the light signal collected by the lens is avoided when imaging the imaging part on one side, thereby ensuring the accuracy of the imaging results of the imaging part of the living animal.

[0023] (3) The present invention provides a fixed structure for adjusting the posture of a living animal, including a plurality of vertical rods and a plurality of horizontal rods. By interlacing the vertical rods with the vertical through holes on the rotating disk in the rotating platform, combined with the axially movable limiting portion on the vertical rods, the height of the vertical rods on the rotating disk can be adjusted. By inserting the horizontal rods into the horizontal through holes at the top ends of the vertical rods, the horizontal length of the horizontal rods extending out of the vertical rods can be adjusted. By inserting the plurality of vertical rods into the vertical through holes at different positions on the rotating disk, different parts of the living animal can be fixed and limited, which is more conducive to the collection of information on the imaging parts of the living animal. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0025] Figure 1 Schematic diagram of the overall structure of the bilateral imaging device for living animals in the present invention;

[0026] Figure 2 This is a schematic diagram of the relative relationship between the initial state of the living animal and the imaging laser and lens;

[0027] Figure 3 A schematic diagram showing the relative relationship between the imaging part on one side of a living animal and the imaging laser and lens;

[0028] Figure 4 A schematic diagram of the relative relationship between the imaging part on the other side of the living animal and the imaging laser and lens;

[0029] Among them, 1. Transmitter; 2. Beam splitter; 3. Light path baffle; 4. Reflector; 5. Lens; 6. Base; 7. Rotating disk; 8. Vertical through hole; 9. Vertical rod; 10. Horizontal rod; 11. Hose; 12. Support rod; 13. Clamp; 14. Living animal; 15. Imaging part; 16. Central axis; 17. Imaging laser. DETAILED DESCRIPTION

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0031] The purpose of the present invention is to provide a bilateral imaging device for living animals and a method for using the same, so as to solve the problems existing in the above-mentioned prior art. In the present invention, by controlling the rotation angle of the rotating platform, the imaging laser is shot into the imaging parts on both sides of the living animal, which can ensure the consistency of the selection of the imaging parts. The time interval between imaging the imaging parts on both sides is shortened as much as possible through the operation of the rotating platform, thereby maximizing the accuracy of the imaging results in the comparative experiment. At the same time, the imaging results of the symmetrical imaging parts on both sides are obtained by receiving the light signal through a lens, which can reduce the cost of experimental equipment.

[0032] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0033] refer to Figures 1 to 3As shown, the present invention provides a bilateral imaging device for a living animal, including an imaging structure and a rotating platform. The imaging structure includes an imaging laser 17 and a lens 5 for receiving a light signal. The imaging laser 17 is symmetrically arranged on both sides of the central axis 16 of the lens 5. Furthermore, the imaging laser 17 is two beams, and the two imaging lasers 17 are symmetrically distributed on both sides of the central axis 16 and coplanar with the central axis 16. The two imaging lasers 17 are obliquely projected and converge at the same position on the central axis 16. Furthermore, the two imaging lasers 17 and the central axis 16 are distributed on the same horizontal plane, and the longitudinal axis of the living animal 14 is aligned with the central axis. 16 is located in the same plane, where the longitudinal axis is the direction of connection between the head and tail of the living animal 14. Furthermore, the vertical plane where the longitudinal axis of the living animal 14 is located is perpendicular to the horizontal plane where the two imaging laser beams 17 are located. The living animal 14 is placed on a rotating platform, and imaging parts 15 are symmetrically provided on both sides of the living animal 14. The imaging parts 15 have a rotation trajectory under the rotation of the rotating platform. By rotating the rotating platform, the imaging part 15 meets the obliquely incident imaging laser 17 on the same side, that is, the imaging laser 17 is injected into the interior of the imaging part 15, and receives the imaging laser beam from the imaging part through the lens 5 arranged in front. When imaging the imaging portion 15 on one side, the rotation angle of the rotating platform from the initial state to the moment it encounters the imaging laser 17 on the same side is recorded, and the rotating platform is rotated in the opposite direction from the initial state by the same angle to image the imaging portion 17 on the other side. By controlling the rotation angle of the rotating platform, the optical signal reflected from the imaging portion 15 can be received by the lens 5 disposed in front of the living animal 14 while the imaging laser 17 enters the imaging portion 15. At the same time, the consistency of the selection of the imaging portion 15 can be ensured. Compared to the prior art method of maintaining the position of the living animal 14 unchanged and using an adjustment instrument to change the optical path, the rotating platform rotates the living animal 14 while maintaining the imaging laser 17 stationary, which can save experimental operating space. At the same time, the operation of the rotating platform can minimize the time interval between imaging the imaging portions 15 on both sides, so that the imaging results collected on both sides are as close to the same state of the living animal 14 as possible, thereby maximizing the accuracy of the imaging results in the comparative experiment. At the same time, the imaging results of the bilaterally symmetrical imaging portions 15 obtained by receiving the optical signal through a single lens 5 can reduce the cost of experimental equipment.

[0034] refer to Figure 1As shown, further, the present invention also includes an anesthesia structure for anesthetizing a living animal 14, the anesthesia structure includes a hose 11 for circulating anesthetic gas and an anesthesia machine for outputting anesthetic gas, one end of the hose 11 is connected to the anesthesia machine, and the other end of the hose 11 is set directly above the nose of the living animal 14. Furthermore, the hose 11 can be connected to the support rod 12 by a clamp 13. By adjusting the angle of the clamp 13 and the position of clamping the hose 11, the other end of the hose 11 is directly above the nose, keeping the nose of the living animal 14 in the anesthesia range of the anesthetic gas, so that the living animal 14 can maintain the adjusted fixed posture during the experiment.

[0035] Furthermore, the imaging structure also includes an optical path adjustment structure, which includes an emitter 1 for emitting imaging laser, a beam splitter 2 for splitting the imaging laser 17, an optical path baffle 3 for shielding the imaging laser 17 on one side after the beam splitting, and a reflector 4 for changing the path of the imaging laser 17. The emitter 1 is coaxially arranged on the central axis 16 facing the living animal 14, the beam splitter 2 is arranged in front of the emitter 1, and the imaging laser 17 emitted by the emitter 1 is symmetrically divided into two beams along the central axis 16. The reflector 4 is symmetrically arranged on the left and right sides. On both sides of the central axis 16, light path baffles 3 are arranged between the reflector 4 and the beam splitter 2. By moving the light path baffle 3 left and right, any one of the two beams of imaging laser 17 can be blocked. The imaging laser 17 not blocked by the light path baffle 3 is emitted into the imaging part 15 of the living animal 14 through the reflector 4. By splitting and blocking the imaging laser 17, combined with the rotation of the living animal 14 by the rotating platform, laser imaging of the imaging parts 15 on both sides of the living animal 14 can be achieved, so that comparative experimental research and analysis of the imaging results can be carried out.

[0036] Furthermore, the rotating platform includes a base 6 and a rotating disk 7. The living animal 14 is placed on the upper surface of the rotating disk 7. A number of vertical through holes 8 are provided between the upper and lower surfaces of the rotating disk 7. The rotating disk 7 is rotatably connected to the base 6 by inserting a pin shaft in the vertical through hole 8. The base 6 may be connected to a vertical pin shaft perpendicular to the plane of the base 6. The vertical through hole 8 on the rotating disk 7 may be sleeved on the vertical pin shaft, or the base 6 may be provided with a plug-in slot. The vertical through hole 8 on the rotating disk 7 is aligned with the plug-in slot, and the vertical pin shaft is inserted into the slot through the vertical through hole 8. The rotating disk 7 is now rotatably connected around the vertical pin, and the vertical through holes 8 are uniformly opened in the radial direction of the rotating disk 7. The rotating disk 7 is a circular disk. When the living animal 14 is placed, the midpoint between the imaging parts 15 on both sides coincides with the rotation center of the rotating disk 7 around the vertical pin. By matching the vertical pin with the vertical through holes 8 at different positions on the rotating disk 7, the size of the use surface of the rotating disk 7 for placing the living animal 14 can be adjusted, so that the vertical through holes 8 at appropriate positions on the rotating disk 7 can be selected according to the size of the living animal 14 to intersperse and cooperate with the vertical pin for rotational connection.

[0037] Furthermore, the present invention also includes a fixed structure for adjusting the posture of a living animal, the fixed structure includes a vertical rod 9 and a horizontal rod 10, the bottom end of the vertical rod 9 is plugged into the vertical through hole 8, the top of the vertical rod 9 is connected to the horizontal rod 10, the bottom end of the vertical rod 9 is provided with a limiting portion that moves along the axial direction of the vertical rod 9, the cross-sectional area of ​​the limiting portion is larger than the cross-sectional area of ​​the vertical through hole 8, the limiting portion can be a rubber sleeve mounted on the outer surface of the vertical rod 9, the rubber sleeve tightly clamps the outer surface of the vertical rod 9, but the position of the rubber sleeve on the vertical rod 9 can be manually adjusted, the cross-sectional area of ​​the rubber sleeve is larger than the cross-sectional area of ​​the vertical through hole 8, to ensure that the vertical rod 9 is inserted into the vertical through hole When the handle is in the hole 8, the rubber sleeve is clamped in the end of the vertical through hole 8, thereby realizing the vertical height adjustment of the vertical rod 9 on the rotating disk 7. The top of the vertical rod 9 is provided with a horizontal through hole for the cross rod 10 to be inserted. The cross rod 10 can be plugged or threaded into the horizontal through hole, thereby changing the horizontal extension length of the cross rod 10 on the vertical rod 9. Furthermore, at least two vertical rods 9 and at least two cross rods 10 are provided. By inserting different vertical rods 9 into the vertical through holes 8 at different positions on the rotating disk 7, the distance between different vertical rods 9 can be adjusted and different positions of the living animal 14 can be fixed.

[0038] The present invention also provides a method for using a bilateral imaging device for a living animal, comprising the following steps:

[0039] Adjust the optical path adjustment structure so that the imaging laser 17 is symmetrically injected from both sides of the central axis 16 of the lens 5 to the same position on the central axis 16; place the living animal 14 on the rotating platform, and adjust the posture of the living animal 14 using the fixed structure to keep the longitudinal axis of the living animal 14 and the central axis 16 in the same plane, and the vertical plane of the longitudinal axis is perpendicular to the plane of the imaging laser 17. Control the anesthesia structure to deliver anesthetic gas to the nose of the living animal 14, keeping the nose within the anesthetic range of the anesthetic gas;

[0040] The rotating platform is rotated from the initial state, and at the intersection of the imaging part 15 of the living animal 14 and the imaging laser 17 on the same side, the imaging laser 17 on the other side is blocked, and the rotation angle of the rotating platform is recorded as A. The optical signal reflected from the imaging part 15 is received through the lens 5, and the rotating platform returns to the initial state; the rotating platform is rotated in the opposite direction from the initial state by an angle A, and at the intersection of the imaging part 15 and the imaging laser 17 on the same side, the imaging laser 17 on the other side is blocked, and the optical signal reflected from the imaging part 15 is received through the lens 5, and the rotating platform returns to the initial state;

[0041] Furthermore, the imaging part 15 is the eye on one side of the living animal 14, and the fixed structure is adjusted to adjust the head and neck of the living animal 14 toward the direction of the lens 5, adjust the height of the vertical rod 9 and the horizontal extension length of the cross rod 10 on the vertical rod 9, and place the head of the living animal 14 between the two vertical rods 9. The two cross rods 10 horizontally interlaced on the two vertical rods 9 are respectively pressed against the ears or other fixed parts of the living animal 14. By adjusting the vertical rods 9 and the cross rods 10, the head and neck of the living animal 14 are adjusted to ensure that the head and neck are facing the lens 5 directly in front, and the eyes on both sides are symmetrically distributed on both sides of the central axis 16.

[0042] Adaptive changes based on actual needs are all within the scope of protection of the present invention.

[0043] It should be noted that it will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the present invention is defined by the appended claims, not the foregoing description, and it is intended that all variations within the meaning and range of equivalents of the claims be encompassed within the present invention. Any reference signs in the claims should not be construed as limiting the claim to which they relate.

Claims

1. A method for using a bilateral imaging device for a living animal, characterized in that: The following steps are involved: Adjusting the optical path adjustment structure so that the imaging laser is symmetrically directed from both sides of the central axis of the lens to the same position on the central axis; placing the living animal on a rotating platform, adjusting the posture of the living animal using the fixed structure so that the longitudinal axis of the living animal and the central axis are located in the same plane, and the vertical plane of the longitudinal axis is perpendicular to the plane of the imaging laser; controlling the anesthesia structure to deliver anesthetic gas to the nose of the living animal, so that the nose is within the anesthetic range of the anesthetic gas; The rotating platform is rotated from the initial state, and at the intersection of the imaging part of the living animal and the imaging laser on the same side, the imaging laser on the other side is blocked, the rotation angle of the rotating platform is recorded as A, and the light signal reflected from the imaging part is received through the lens, and the initial state of the rotating platform is returned; the rotating platform is rotated in the opposite direction from the initial state by an angle A, and at the intersection of the imaging part and the imaging laser on the same side, the imaging laser on the other side is blocked, and the light signal reflected from the imaging part is received through the lens, and the initial state of the rotating platform is returned; The invention also includes a bilateral imaging device for a living animal, wherein the bilateral imaging device for a living animal includes an imaging structure and a rotating platform, wherein the imaging structure includes an imaging laser and a lens for receiving light signals, wherein the imaging lasers are symmetrically arranged on both sides of the central axis of the lens, wherein the longitudinal axis of the living animal and the central axis are located in the same plane, wherein the plane where the longitudinal axis is located is perpendicular to the plane where the imaging lasers are located, and the living animal is placed on the rotating platform; the bilateral imaging device for a living animal also includes an anesthesia structure for the living animal, wherein the anesthesia structure includes a hose for circulating anesthesia gas and an anesthesia machine for outputting the anesthesia gas. One end of the hose is connected to the anesthesia machine, and the other end of the hose is arranged just above the nose of the living animal; the imaging structure also includes an optical path adjustment structure, which includes an emitter for emitting the imaging laser, a beam splitter for splitting the imaging laser, an optical path baffle for blocking the imaging laser on one side after the splitting, and a reflector for changing the imaging laser path; the living animal bilateral imaging device also includes a fixed structure for adjusting the posture of the living animal, the fixed structure includes a vertical rod and a horizontal rod, the bottom end of the vertical rod is plugged into the vertical through hole, and the top end of the vertical rod is connected to the horizontal rod.

2. The method for using the bilateral imaging device for living animals according to claim 1, characterized in that: The emitter is coaxially arranged on the central axis toward the living animal, the beam splitter is arranged directly in front of the emitter, and the reflectors are symmetrically arranged on both sides of the central axis.

3. The method for using the bilateral imaging device for living animals according to claim 1, characterized in that: The rotating platform includes a base and a rotating disk. The living animal is placed on the upper surface of the rotating disk. A plurality of vertical through holes are opened between the upper and lower surfaces of the rotating disk. The rotating disk is rotatably connected to the base by inserting pins in the vertical through holes.

4. The method for using the bilateral imaging device for living animals according to claim 3, characterized in that: The vertical through holes are uniformly opened in the circumferential direction along the radial direction of the rotating disk.

5. The method for using the bilateral imaging device for living animals according to claim 1, characterized in that: The bottom end of the vertical rod is provided with a limiting portion that moves axially along the vertical rod. The cross-sectional area of ​​the limiting portion is larger than the cross-sectional area of ​​the vertical through hole. The top end of the vertical rod is provided with a horizontal through hole for the cross rod to pass through.

6. The method for using the bilateral imaging device for living animals according to claim 1, characterized in that: The imaging part is an eye on one side of the living animal, and the fixing structure adjusts the head and neck of the living animal toward the lens.

Images