A magnetic microbead coated separation method

Abstract

The application discloses a magnetic microbead coating separation method, which comprises a feeding channel, a mixing and magnetic separation platform, a reaction incubation platform, a discharging channel, a suction liquid platform and a transfer device. The suction liquid platform is used for sucking and sucking liquid from a container. The transfer device is used for transferring the container in the feeding channel to the mixing and magnetic separation platform to perform mixing and magnetic separation processes. The transfer device is used for transferring the container to the reaction incubation platform to perform incubation. The transfer device is also used for transferring the container to the discharging channel. Compared with the prior art, the application can meet the needs of large-scale and automatic production, greatly improve the yield and efficiency of magnetic microbead coating separation, and meet the production needs.

Description

Technical Field

[0001] The invention belongs to the technical field of magnetic microbead coating separation, and in particular relates to a magnetic microbead coating separation device. Background Art

[0002] The diagnostic reagent industry has developed rapidly in recent years. Diagnostic reagents can be used to quickly and accurately test and diagnose the cause of a disease. Blood samples can also be collected at one time to test multiple indicators, which helps medical personnel to formulate targeted treatment plans and provide accurate medical treatment and medication.

[0003] In the process of development and industrialization of diagnostic reagents, magnetic microbead coating and separation is its core process, and the demand for diagnostic reagents has shown explosive growth.

[0004] In the existing technology, magnetic microbead coating separation is generally performed manually, which is inefficient and cannot meet production needs. The existing technology also includes methods that use small magnetic microparticle coating devices to achieve magnetic microbead coating separation, but this still cannot meet the needs of large-scale and automated production.

[0005] Therefore, it is necessary to provide a new magnetic microbead coating separation method to solve the above technical problems. Summary of the Invention

[0006] (1) Technical issues to be resolved

[0007] Based on this, the present invention provides a magnetic microbead coating separation device to solve the technical problem that the existing magnetic microbead coating separation method cannot meet the needs of large-scale and automated production.

[0008] (2) Technical solution

[0009] In order to solve the above technical problems, the present invention proposes a magnetic microbead coated separation device, including a feed channel, a mixing magnetic separation platform, a reaction incubation platform, a discharge channel, a liquid suction platform and a transfer device. The liquid suction platform is used to extract and aspirate liquid from the container, and the transfer device is used to transfer the container in the feed channel to the mixing magnetic separation platform for mixing and magnetic separation processes, and the transfer device is used to transfer the container to the reaction incubation platform for incubation, and the transfer device is also used to transfer the container to the discharge channel.

[0010] Preferably, before the container is transferred to the discharge channel, the transfer device repeatedly transfers the container between the mixing magnetic separation platform and the reaction incubation platform to achieve multiple mixing and magnetic separation processes and incubation processes.

[0011] Preferably, the transport device comprises a first robot for transporting the container in the feed channel to the mixing magnetic separation platform and a second robot for transporting the container to the reaction incubation platform.

[0012] Preferably, there are multiple reaction incubation platforms, and the multiple reaction incubation platforms are arranged around the side of the second robot away from the first robot; there are multiple mixing magnetic separation platforms and the multiple mixing magnetic separation platforms are arranged side by side, and the liquid aspiration platform includes a filling robot and a pump group arranged on one side of the mixing magnetic separation platform.

[0013] Preferably, a handover platform is provided between the first robot and the second robot, and a coating tube accommodating slot is provided on the top of the handover platform, and the number of the coating tube accommodating slots is the same as the number of the mixing magnetic separation platforms.

[0014] Preferably, the mixing magnetic separation platform includes: a magnetic separation tank, a magnetic ring arranged around the magnetic separation tank, and a lifting column arranged at the lower part of the magnetic ring and used to drive the magnetic ring close to or away from the magnetic separation tank, and the lifting column is also used to drive the magnetic ring to rotate.

[0015] Preferably, the mixing magnetic separation platform also includes a jacket provided outside the lifting column and rotatably connected to the lifting column, a bearing is provided between the jacket and the lifting column, the magnetic separation accommodating tank is provided at the top of the jacket, and the mixing magnetic separation platform also includes an outer column arranged around the jacket.

[0016] Preferably, each of the reaction incubation platforms includes a mounting seat and a shaking device provided on the mounting seat, the shaking device includes shaking tanks, the number of the shaking tanks is the same as the number of the mixing magnetic separation platforms, a shaking rotating power component for driving the shaking tanks to rotate is provided at the bottom of the shaking device, a protective cover is provided at the top of the shaking tank, and a shield driving power component for driving the protective cover to move up and down and rotate is provided on one side of the mounting seat.

[0017] Preferably, the filling robot includes: a rotating base, a filling needle moving device and a filling needle assembly. A rotating shaft is provided at the lower part of one end of the rotating base. The filling needle assembly is arranged on the upper part of the rotating base through the filling needle moving device. The filling needle moving device includes: a moving guide rail and a moving box slidably connected to the moving guide rail. The filling needle assembly is fixed on the moving box.

[0018] Preferably, the filling needle assembly includes a plurality of filling needles arranged side by side, and the filling needle assembly further includes a filling needle lifting device respectively connected to each of the filling needles and used for driving the filling needles to rise and fall.

[0019] (3) Beneficial effects

[0020] Compared with the prior art, the magnetic microbead coating separation method of the present invention has the following advantages:

[0021] In the present invention, the transfer device is used to realize the automatic transfer of materials. The feed channel, the mixing magnetic separation platform, the reaction incubation platform, the discharge channel, the suction liquid platform and the transfer device together constitute the magnetic microbead coating machine. The feed channel is used for feeding the container, and the mixing magnetic separation platform is used to mix and magnetically separate the liquid in the container. The reaction incubation platform is used to realize incubation. The discharge channel is used for discharging. The suction liquid platform is used to extract the liquid in the container and inject the liquid medicine into the container. The various functional components are used in combination to meet the needs of large-scale and automated production, which can greatly improve the output and efficiency of magnetic microbead coating separation and meet production needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0023] Figure 1 Schematic diagram of the overall structure of the magnetic microbead coating machine used in the present invention;

[0024] Figure 2 Schematic diagram of the structure of the mixing magnetic separation platform in the magnetic microbead coating machine used in the present invention;

[0025] Figure 3 Schematic diagram of the structure of the reaction incubation platform in the magnetic microbead coating machine used in the present invention;

[0026] Figure 4 This is a structural diagram of the filling robot in the magnetic microbead coating machine used in the present invention;

[0027] Figure 5 for Figure 4 Left view of .

[0028] Description of reference numerals:

[0029] 100. Package tube;

[0030] 1. Coating management machine, 2. Feed channel, 3. First robot, 4. Handover station, 5. Second robot, 6. Reaction incubation platform; 7. Capping and discharging system, 8. Mixing magnetic separation platform, 9. Filling robot, 10. Pump group;

[0031] 41. Coated tube storage tank;

[0032] 61. Mounting seat, 62. Shaking device, 63. Protective cover, 64. Protective cover driving power part;

[0033] 81. Magnetic separation accommodating tank, 82. Magnetic ring, 83. Lifting column, 84. Jacket, 85. Bearing, 86. External column;

[0034] 91. Rotating base, 92. Rotating shaft, 93. Moving guide rail, 94. Moving box, 95. Filling needle, 96. Filling needle lifting device. DETAILED DESCRIPTION

[0035] To make the above-mentioned objects, features, and advantages of the present invention more readily apparent, specific embodiments of the present invention are described in detail below with reference to the accompanying drawings. The following description sets forth numerous specific details to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways than those described herein, and those skilled in the art may make similar modifications without departing from the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0036] The following is combined with Figure 1-5 The magnetic microbead coating separation method of the present invention is further described.

[0037] Please focus on Figure 1 The present invention discloses a magnetic microbead coating separation method, which includes a feed channel 2, a mixing magnetic separation platform 8, a reaction incubation platform 6, a discharge channel, a liquid suction platform and a transfer device. The liquid suction platform is used to extract and aspirate liquid from the container, and the transfer device is used to transfer the container in the feed channel 2 to the mixing magnetic separation platform 8 for mixing and magnetic separation processes, and the transfer device is used to transfer the container to the reaction incubation platform 6 for incubation, and the transfer device is also used to transfer the container to the discharge channel.

[0038] More specifically, before the container is transferred to the discharge channel, the transfer device repeatedly transfers the container between the mixing magnetic separation platform 8 and the reaction incubation platform 6 to achieve multiple mixing and magnetic separation processes and incubation processes.

[0039] In this embodiment, the container is specifically a cryopreservation tube 100. The feed channel 2 is used for feeding the cryopreservation tube, and the mixing magnetic separation platform 8 is used for mixing and magnetically separating the liquid in the container. The reaction incubation platform 6 is used to achieve incubation. The discharge channel is used for discharging. The suction liquid platform is used to extract the liquid in the container and inject the liquid medicine into the container. The transfer device is used to realize the automatic transfer of materials. The feed channel 2, the mixing magnetic separation platform 8, the reaction incubation platform 6, the discharge channel, the suction liquid platform and the transfer device together constitute a magnetic microbead coating machine. The various functional components are used in combination to meet the needs of large-scale and automated production, which can greatly improve the output and efficiency of magnetic microbead coating separation and meet production needs.

[0040] According to a specific embodiment of the present invention, the transfer device includes a first robot 3 for transferring the container in the feed channel 2 to the mixing magnetic separation platform 8 and a second robot 5 for transferring the container to the reaction incubation platform 6 .

[0041] In this embodiment, the transportation is performed by two independent robots, which can further improve the transportation efficiency and make the movement requirements of a single robot simpler, thereby ensuring the reliability of the robot movement.

[0042] According to a specific embodiment of the present invention, there are multiple reaction incubation platforms 6, and the multiple reaction incubation platforms 6 are arranged around the side of the second robot 5 away from the first robot 3; there are multiple mixing magnetic separation platforms 8 and the multiple mixing magnetic separation platforms 8 are arranged side by side, and the liquid suction platform includes a filling robot 9 and a pump group 10 arranged on one side of the mixing magnetic separation platform 8.

[0043] According to a specific embodiment of the present invention, a handover platform 4 is provided between the first robot 3 and the second robot 5, and a cryotube accommodating slot 41 is provided on the top of the handover platform 4. The number of the cryotube accommodating slots 41 is the same as the number of the mixing magnetic separation platforms 8.

[0044] In this embodiment, the first robot 3 transfers the container to the transfer station 4 , and the second robot 5 transfers the container on the transfer station 4 to the reaction incubation platform 6 .

[0045] Please focus on Figure 2 According to a specific embodiment of the present invention, the mixing magnetic separation platform 8 includes: a magnetic separation tank 81, a magnetic ring 82 arranged around the magnetic separation tank 81, and a lifting column 83 provided at the lower part of the magnetic ring 82 and used to drive the magnetic ring 82 close to or away from the magnetic separation tank 81. The lifting column 83 is also used to drive the magnetic ring 82 to rotate.

[0046] In this embodiment, the magnetic separation tank 81 is used to place the cryotube 100, and the magnetic ring 82 surrounds the cryotube 100 to achieve magnetic microbead coating separation. When the lifting column 83 drives the magnetic ring 82 to rotate, it can carry the cryotube 100 to rotate, thereby achieving mixing.

[0047] According to a specific embodiment of the present invention, the mixing magnetic separation platform 8 also includes a jacket 84 arranged outside the lifting column 83 and rotatably connected to the lifting column 83, a bearing 85 is provided between the jacket 84 and the lifting column 83, and the magnetic separation tank 81 is provided at the top of the jacket 84. The mixing magnetic separation platform 8 also includes an outer column 86 arranged around the jacket 84.

[0048] In this embodiment, the bearing 85 is provided to improve the flexibility of the lifting column 83 and enhance the mixing effect. The jacket 84 and the outer column 86 cooperate to achieve stable support of the lifting column 83.

[0049] Please focus on Figure 3 According to a specific embodiment of the present invention, each reaction incubation platform 6 includes a mounting seat 61 and a shaking device 62 provided on the mounting seat 61, the shaking device 62 includes a shaking tank, and the number of the shaking tanks is the same as the number of the mixing magnetic separation platform 8. A shaking rotating power part for driving the shaking tank to rotate is provided at the bottom of the shaking device 62, and a protective cover 63 is provided at the top of the shaking tank. A shield driving power for driving the protective cover 63 to move up and down and rotate is provided on one side of the mounting seat 61.

[0050] In this embodiment, the shaking tank is used to accommodate the cryopreservation tubes 100, and each reaction incubation platform 6 includes the same number of shaking tanks as the mixing magnetic separation platform 8. This ensures that the coated tubes 100 subjected to magnetic separation in the same batch are all installed in the same reaction incubation platform 6, which is beneficial to improving production efficiency. After the coated tubes 100 are loaded into the shaking tanks, the protective cover 63 covers the shaking tanks under the action of the shield driving power member 64 to ensure that the incubation environment meets the requirements. When the incubation is completed, the shield driving power member 64 lifts the protective cover 63, then rotates it open, and no longer covers the shaking tank, making it convenient for the robot to transport the coated tubes 100 in the shaking tank.

[0051] Please focus on Figure 4-5 According to a specific embodiment of the present invention, the filling robot 9 includes: a rotating base 91, a filling needle moving device and a filling needle assembly. A rotating shaft 92 is provided at the lower part of one end of the rotating base 91. The filling needle assembly is arranged on the upper part of the rotating base 91 through the filling needle moving device. The filling needle moving device includes: a moving guide rail 93 and a moving box 94 slidably connected to the moving guide rail 93. The filling needle assembly is fixed on the moving box 94.

[0052] More specifically, the filling needle assembly includes a plurality of filling needles 95 arranged side by side, and the filling needle assembly also includes a filling needle lifting device 96 connected to each filling needle 95 and used to drive the filling needle 95 to rise and fall.

[0053] More specifically, the pump assembly 10 includes a plurality of injection pumps and a displacement pump arranged side by side.

[0054] In this embodiment, the filling needle assembly is used to insert into the cryopreservation tube 100 to extract liquid, or to inject various liquids into the cryopreservation tube 100. The filling needle 95 is driven to rise and fall by the filling needle lifting device 96, so that the filling needle 95 can be inserted downward into the coating tube 100 or removed upward from the coating tube 100. The movable box 94 slides along the movable guide rail 93, which can drive the entire filling needle assembly to move horizontally, so that the filling needle 95 can inject or extract liquid into each mixing magnetic separation platform 8 in turn. The rotating base 91 rotates around the rotating shaft 92, so that the filling needle assembly can be moved between the pump group 10 and the mixing magnetic separation platform 8.

[0055] In this embodiment, the injection pumps are used to inject antigen, liquid A, liquid B, liquid C, liquid D, liquid E, and liquid F. The drainage pump is used to discharge the extracted waste liquid. Liquid A, liquid B, liquid C, liquid D, liquid E, and liquid F are six different liquids added to the coating tube 100. The lack of disclosure of their specific compositions would hinder the full disclosure of the magnetic microbead coating separation method of this application.

[0056] More specifically, a screw capping and discharging system 7 is provided on the discharging channel; the magnetic microbead coating machine also includes a freezing management machine 1 connected to the feeding channel 2.

[0057] In this embodiment, the capping and discharging system 7 is used to cap, screw cap and discharge the cryotubes 100. The cryotube management machine 1 is used to sort out the disordered cryotubes 100 and output them one by one in a single row to the feeding channel 2.

[0058] More specifically, the magnetic microbead coating separation method includes the following steps.

[0059] S1, manually or mechanically place the coated tubes 100 into the cryopreservation tube management machine 1, use the cryopreservation tube management machine 1 to sort out the disordered cryopreservation tubes 100, and output them one by one in a single row to the feed channel 2.

[0060] S2, the first robot 3 sequentially grabs n cryopreservation tubes 100, places them on n mixing magnetic separation platforms 8 and performs the front-end mixing magnetic separation process, where n is a natural number greater than 1 and less than 10 (see Appendix Figure 1 n=5 shown in ).

[0061] S3 , the first robot 3 sequentially grabs the n cryotubes 100 on the mixing magnetic separation platform 8 and places them on the transfer station 4 .

[0062] S4, the second robot 5 sequentially grabs n cryotubes 100 at the transfer station 4 and places them on a reaction incubation platform 6, and performs one incubation.

[0063] S5, repeating the above steps S2-S3 until the remaining freezing tubes 100 of the reaction incubation platform 6 are filled.

[0064] S6. After one incubation is completed, the second robot 5 places the n coated tubes 100 on an incubation platform on the handover table 4. The first robot 3 places the cryopreservation tubes 100 on the uniform magnetic separation platform and performs the mid-section mixing magnetic separation process. Then, the first robot 3 places the cryopreservation tubes 100 on the handover table 4, and then the second robot 5 places them back to the incubation platform for a second incubation.

[0065] S7, repeat the above step S6, so that the remaining cryopreservation tubes 100 in the incubation platform complete the middle mixing and magnetic separation process, and are returned to the incubation platform for a second incubation.

[0066] S8, after the second incubation is completed, the second robot 5 places the n coated tubes 100 on an incubation platform on the transfer platform 4, and the first robot 3 mixes the cryopreservation tubes 100 and places them on the uniform magnetic separation platform for the subsequent uniform magnetic separation process.

[0067] S9, the first robot 3 sequentially grabs n cryopreservation tubes 100 and places them into the capping and discharging system 7 to complete the capping, capping and discharging processes.

[0068] S10, repeat the above steps S8-S9 to ensure that the remaining cryopreservation tubes 100 in the incubation platform are completely mixed and magnetically separated, and are placed in the capping and discharging system 7 to complete the capping, capping and bottle discharging processes.

[0069] More specifically, in step S2, the front-end mixing and magnetic separation process includes the following steps in sequence: adding magnetic beads, magnetic separation, discarding the supernatant, withdrawing the magnetic field, adding liquid A, mixing, adding antigen, adding liquid B, and adding liquid C; in step S6, the middle-end mixing and magnetic separation process includes the following steps in sequence: magnetic separation, discarding the supernatant, withdrawing the magnetic field, adding liquid D, and mixing; in step S8, the back-end mixing and magnetic separation process includes the following steps in sequence: magnetic separation, discarding the supernatant, withdrawing the magnetic field, adding liquid E, and mixing.

[0070] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "connected" and "connection" should be understood in a broad sense. For example, it can mean a fixed connection, a detachable connection, or an integral connection; it can mean a mechanical connection, an electrical connection, a direct connection, or an indirect connection through an intermediate medium; it can mean internal communication between two elements, or a "transmission connection," i.e., a power connection through various appropriate means such as a belt drive, a gear drive, or a sprocket drive. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances.

Claims

1. A magnetic microbead coating separation device, comprising a feed channel (2), a plurality of mixing magnetic separation platforms (8), a reaction incubation platform (6), a discharge channel, a liquid suction platform and a transport device, characterized in that: The liquid aspiration platform is used to aspirate and aspirate liquid from the container, the transport device is used to transport the container in the feed channel (2) to the mixing magnetic separation platform (8) for mixing and magnetic separation, the transport device is used to transport the container to the reaction incubation platform (6) for incubation, and the transport device is used to transport the container to the discharge channel. The liquid aspiration platform can sequentially inject or extract liquid from each mixing magnetic separation platform (8); The transport device comprises a first robot (3) for transporting the container in the feed channel (2) to the mixing magnetic separation platform (8) and a second robot (5) for transporting the container to the reaction incubation platform (6); A handover platform (4) is provided between the first robot (3) and the second robot (5), and a coating tube accommodating slot (41) is provided on the top of the handover platform (4), and the number of the coating tube accommodating slots (41) is the same as the number of the mixing magnetic separation platforms (8).

2. The magnetic microbead coating separation device according to claim 1, characterized in that: Before the container is transferred to the discharge channel, the transfer device repeatedly transfers the container between the mixing magnetic separation platform (8) and the reaction incubation platform (6) to achieve multiple mixing and magnetic separation processes and incubation processes.

3. The magnetic microbead coating separation device according to claim 2, characterized in that: There are multiple reaction incubation platforms (6), and the multiple reaction incubation platforms (6) are arranged around the side of the second robot (5) away from the first robot (3); there are multiple mixing magnetic separation platforms (8), and the multiple mixing magnetic separation platforms (8) are arranged side by side, and the liquid aspiration platform includes a filling robot (9) and a pump group (10) arranged on one side of the mixing magnetic separation platform (8).

4. The magnetic microbead coating separation device according to claim 3, characterized in that: The mixing magnetic separation platform (8) comprises: a magnetic separation accommodating tank (81), a magnetic ring (82) arranged around the magnetic separation accommodating tank (81), and a lifting column (83) arranged at the bottom of the magnetic ring (82) and used to drive the magnetic ring (82) to approach or move away from the magnetic separation accommodating tank (81), and the lifting column (83) is also used to drive the magnetic ring (82) to rotate.

5. The magnetic microbead coating separation device according to claim 4, characterized in that: The mixing magnetic separation platform (8) further includes a jacket (84) arranged outside the lifting column (83) and rotatably connected to the lifting column (83), a bearing (85) is provided between the jacket (84) and the lifting column (83), the magnetic separation accommodating groove (81) is provided at the top of the jacket (84), and the mixing magnetic separation platform (8) further includes an outer column (86) arranged around the jacket (84).

6. The magnetic microbead coating separation device according to claim 5, characterized in that: Each of the reaction incubation platforms (6) includes a mounting seat (61) and a shaking device (62) provided on the mounting seat (61), the shaking device (62) includes a shaking accommodating tank, the number of the shaking accommodating tanks being the same as the number of the mixing magnetic separation platforms (8), a shaking rotating power member for driving the shaking accommodating tanks to rotate is provided at the bottom of the shaking device (62), a protective cover (63) is provided at the top of the shaking accommodating tank, and a shield driving power member (64) for driving the protective cover (63) to move up and down and rotate is provided on one side of the mounting seat (61).

7. The magnetic microbead coating separation device according to claim 6, characterized in that: The filling manipulator (9) comprises: a rotating base (91), a filling needle moving device and a filling needle assembly. A rotating shaft (92) is provided at the lower part of one end of the rotating base (91). The filling needle assembly is arranged on the upper part of the rotating base (91) via the filling needle moving device. The filling needle moving device comprises: a moving guide rail (93) and a moving box (94) slidably connected to the moving guide rail (93). The filling needle assembly is fixed on the moving box (94).

8. The magnetic microbead coating separation device according to claim 7, characterized in that: The filling needle assembly comprises a plurality of filling needles (95) arranged side by side, and the filling needle assembly further comprises a filling needle lifting device (96) respectively connected to each of the filling needles (95) and used for driving the filling needles (95) to rise and fall.

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