A squalane-based nanolipid carrier loaded with ethyl ferulate, and a preparation method and application thereof

Abstract

The application discloses a squalane-based nanolipid carrier loaded with ethyl ferulate and a preparation method and application thereof. The squalane-based nanolipid carrier loaded with ethyl ferulate comprises the following components in percentage by weight: squalane 4-8%; solid oil 4-8%; ethyl ferulate 0.1-1.2%; non-ionic surfactant 1.5-5.5%; alcohol solvent 0.3-0.8%; and deionized water. The squalane-based nanolipid carrier loaded with ethyl ferulate can well load ferulate lipids, and the embedding rate is 90-97%. The particle size of the squalane-based nanolipid carrier loaded with ethyl ferulate is 100-200 nm, and the dispersion index is 0.22-0.26. The squalane-based nanolipid carrier loaded with ethyl ferulate has the advantages of simple preparation method, good particle size, dispersibility, transdermal efficiency and stability.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of skin care products, and particularly relates to a squalane-based nanolipid carrier loaded with ethyl ferulate as well as a preparation method and application thereof. BACKGROUND

[0002] Squalane is an excellent oil extracted from the body of deep-sea sharks, which has the effects of protecting the skin and resisting oxidation. Squalane is a lipid very close to human sebum, has strong affinity, and can form a natural protective barrier on the surface of the skin. Squalane can also inhibit the peroxidation of skin lipids and promote the proliferation of skin basal cells, and has obvious physiological effects on improving and eliminating chloasma. Ethyl ferulate is a natural cosmetic functional ingredient extracted from Chinese herbal medicine angelica, which can scavenge and inhibit oxygen free radicals to play an anti-aging role, and can also inhibit tyrosinase to reduce the generation of melanin, thereby achieving the effect of whitening. In addition, ethyl ferulate can reduce skin damage caused by ultraviolet radiation and can be used as a sunscreen agent in cosmetics. However, the water solubility of both is poor, and ethyl ferulate is in powder form, which limits their application in water-alcohol system cosmetics, and their skin penetration rate in cosmetics is poor. Therefore, squalane is prepared into a nanolipid carrier as a liquid oil, and ethyl ferulate is loaded therein, which can solve the problem of poor water solubility of both, and improve the skin penetration rate of ethyl ferulate.

[0003] For example, CN112842924A discloses a preparation method of squalane liposomes, which comprises 20-20% squalane, 3-8% caprylic / capric acid glycerol triester, 5-6% phytosterol, 10-15% hydrogenated lecithin, 3% 1,2-hexanediol, and deionized water supplemented to 100%. The squalane liposomes are mild, do not contain thickening agents, and can supplement the skin barrier. The preparation method needs to be placed in a refrigerator in advance, then taken out and warmed, and the cycle is repeated for 3-6 times. Vacuum and nitrogen are also required in the preparation process. The preparation method is relatively complicated and has a higher cost. Meanwhile, 10-15% hydrogenated lecithin is selected as an emulsifier, the proportion of the emulsifier is high, and there is a risk of damaging the skin surface. Meanwhile, the hydrogenated lecithin will make the emulsion color yellow, resulting in poor product appearance, which is difficult for consumers to accept. Moreover, the particle size of the liposomes prepared by the method is large, which reduces the transdermal ability of the liposomes. SUMMARY

[0004] In view of the deficiencies of the prior art, the purpose of the present application is to provide a squalane-based nanolipid carrier loaded with ethyl ferulate, which is prepared by combining squalane, solid oil, and nonionic surfactant to embed ethyl ferulate, thereby solving the above problems. The nanolipid carrier does not contain lecithin, has the advantages of good water solubility, high embedding rate, small particle size, high transdermal rate, and pure white appearance, and the preparation method is relatively simple.

[0005] Another object of the present application is to provide a preparation method of squalane-based nanolipid carriers loaded with ethyl ferulate, and the nanolipid carriers prepared by the method have the advantages of good water solubility, high embedding rate and good stability.

[0006] The object of the present application is achieved by the following technical solutions.

[0007] A squalane-based nanolipid carrier loaded with ethyl ferulate comprises the following components by weight percentage:

[0008] Squalane 4-8%;

[0009] Solid oil 4-8%;

[0010] Ethyl ferulate 0.1-1.2%;

[0011] Non-ionic surfactant 1.5-5.5%;

[0012] Alcohol solvent 0.3-0.8%;

[0013] The balance is deionized water.

[0014] By adopting the above technical solutions, the squalane-based nanolipid carrier loaded with ethyl ferulate mainly comprises squalane, solid oil, surfactant, alcohol solvent, deionized water, etc.

[0015] Preferably, the solid oil is selected from any one of succinylated monostearate, monolaurate, cetyl alcohol, monoglyceride, glycerol distearate and glycerol behenate.

[0016] Further preferably, the solid oil is succinylated monostearate.

[0017] Preferably, the non-ionic surfactant is selected from any one of Tween-20, poloxamer 188, poloxamer 407, Tween-40 and Span 20.

[0018] Further preferably, the non-ionic surfactant is Tween-20.

[0019] Preferably, the alcohol solvent is selected from any one of phenoxyethanol, benzyl alcohol and cinnamyl alcohol.

[0020] Preferably, the particle size of the squalane-based nanolipid carrier loaded with ethyl ferulate is 100-200 nm.

[0021] Preferably, the embedding rate of ethyl ferulate in the squalane-based nanolipid carrier loaded with ethyl ferulate is greater than 90%.

[0022] Preferably, the dispersion index of the squalane-based nanolipid carrier loaded with ethyl ferulate is 0.22-0.26.

[0023] Preferably, in the components of the squalane-based nanolipid carrier loaded with ethyl ferulate, the mass ratio of solid oil to squalane and deionized water is 0.11-0.31.

[0024] The preparation method of the squalane-based nanolipid carrier loaded with ethyl ferulate described above comprises the following steps:

[0025] (1) Mix squalane, solid oil and ethyl ferulate and heat at a constant temperature to form an oil phase;

[0026] (2) Mix deionized water and non-ionic surfactant and heat at a constant temperature to form an aqueous phase;

[0027] (3) Mix the aqueous phase into the oil phase and heat at a constant temperature to form a mixed phase;

[0028] (4) Homogenize the mixed phase by using a homogenizer at a speed of 9000-15000 rpm for 5-10 min to form a mixed phase emulsion;

[0029] (5) Add an alcohol solvent to the mixed phase emulsion and perform ultrasonic treatment on the mixed phase emulsion by using an ultrasonic instrument to obtain the squalane-based nanolipid carrier loaded with ethyl ferulate.

[0030] Preferably, the temperature for constant heating in step (1) is 90-95℃;

[0031] Preferably, the temperature for constant heating in step (2) is 75-80℃;

[0032] Preferably, the temperature for constant heating in step (3) is 90-95℃;

[0033] Preferably, the power for ultrasonic treatment in step (5) is 240-300 W for 10-20 min.

[0034] The squalane-based nanolipid carrier loaded with ethyl ferulate described above is used in the preparation of skin care products.

[0035] By using the above technical solution, in order to improve the water solubility and transdermal efficiency of squalane and ethyl ferulate, the squalane-based nanolipid carrier loaded with ethyl ferulate is prepared by using a homogenization ultrasonic method, which can improve its water solubility, encapsulation efficiency and stability, avoid the formation of complete crystal lattice of lipids, and expel the drug. The periphery of ethyl ferulate is coated by squalane and solid oil, which can effectively penetrate the skin and reduce drug leakage.

[0036] By adopting the technical scheme, the prepared nanometer lipid carrier has small particle size, increases the contact area of skin and skin care products, and has the effect of preventing moisture from being lost from the skin, and the lipophilic coating outer membrane can effectively isolate the active ingredients from unstable factors in the outside world, strengthen the skin friendliness, and enable the effective ingredients to accurately reach the skin bottom layer, continuously release the effective ingredients, and give the skin the most complete nutrients. The nanometer lipid carrier has a shielding effect. As for the 200nm and 2mu m lipid carriers, the pores between the large particles are relatively large, the ratio is only 20%, and water evaporation is easy to occur. The pores between the small particles are relatively small, the ratio can reach 60%, so water evaporation is not easy to occur. The smaller the particle size, the stronger the adhesion, and the better the shielding effect.

[0037] By adopting the technical scheme, the oil ratio in the application is 8% to 16%, which is much higher than 1.2% of ethyl ferulate. Since ethyl ferulate is fat-soluble, it can be effectively dissolved in the mixed emulsion of oil, thereby having a high embedding rate.

[0038] The application solves the problem that ethyl ferulate and squalane are difficult to be directly applied to skin care products due to poor water solubility, and improves the skin penetration rate of ethyl ferulate. In the application, squalane is used as the only liquid oil, and ethyl ferulate is loaded by cooperating with solid oil and non-ionic surfactant and other raw materials, so that squalane and ferulic acid have good water solubility, thereby the application can be directly applied to water-alcohol system skin care products, and has better transdermal efficiency and other advantages. The liposome system in the application can well load ferulic acid lipid, and the embedding rate is 90% to 97%. The particle size of the squalane-based nanometer lipid loaded with ethyl ferulate is 100nm to 200nm, and the dispersion index is 0.22 to 0.26, which has good particle size, dispersity and stability.

[0039] Compared with the prior art, the application has the following advantages and beneficial effects:

[0040] (1) In the application, squalane is used as the only liquid oil, and ethyl ferulate is loaded by cooperating with solid oil, non-ionic surfactant and other raw materials, so that squalane and ethyl ferulate have good water solubility, and can be applied to water-alcohol system skin care products, so as to have better transdermal efficiency and stability;

[0041] (2) The wrapping system in the application can well load ethyl ferulate, and the loading rate is greater than 90%;

[0042] (3) The squalane-based nanometer lipid loaded with ethyl ferulate prepared in the application has a particle size of 100nm to 200nm and a dispersion index of 0.22 to 0.26, which has good small particle size and dispersity. BRIEF DESCRIPTION OF DRAWINGS

[0043] Figure 1 Transmission electron micrograph of Example 1.

[0044] Figure 2 In-vitro transdermal test graph of Example 1 and Comparative Example 1.

[0045] Figure 3 UV absorption test graph of Example 1, Comparative Example 3 and ethyl ferulate. DETAILED DESCRIPTION

[0046] The present application is further illustrated by the following examples, which do not limit the practical implementation and protection of the present application. It should be noted that if the following processes are not specifically described in detail, they can be implemented or understood by those skilled in the art with reference to the prior art. If the reagents or instruments used are not marked with the manufacturer, they are considered to be conventional products that can be purchased on the market.

[0047] Example 1

[0048] A squalane-based nanolipid carrier loaded with ethyl ferulate, comprising the following components by weight percentage: squalane 8%; monoglyceride 8%; ethyl ferulate 0.5%; Tween-20 5.5%; phenoxyethanol 0.8%; and the balance being deionized water.

[0049] A squalane-based nanolipid carrier loaded with ethyl ferulate, comprising the following specific steps:

[0050] Step one, mix squalane, monoglyceride and ethyl ferulate and heat to 95°C to form an oil phase;

[0051] Step two, mix water and Tween-20 and heat to 80°C to form an aqueous phase;

[0052] Step three, add the aqueous phase to the oil phase and mix evenly, and heat to 90°C to form a mixed phase;

[0053] Step four, use a homogenizer to homogenize and emulsify the mixed phase at a speed of 12000 rpm for 8 minutes to form a mixed phase emulsion.

[0054] Step five, add phenoxyethanol to the mixed phase emulsion and use an ultrasonic instrument to ultrasonically treat the mixed phase emulsion at a power of 90% (maximum power 300W) for 20 minutes to obtain a squalane-based nanolipid carrier loaded with ethyl ferulate.

[0055] The transmission electron micrograph of the squalane-based nanolipid carrier loaded with ethyl ferulate obtained in this example is shown in Figure 1 The particle size and dispersion index are shown in Table 1.

[0056] Example 2

[0057] A squalane-based nanolipid carrier loaded with ethyl ferulate, comprising the following components by weight percentage: squalane 6%; monolauric acid stearate 6%; ethyl ferulate 0.3%; poloxamer 188 4%; phenoxyethanol 0.6%; and the balance being deionized water.

[0058] A squalane-based nanolipid carrier loaded with ethyl ferulate, comprising the following specific steps:

[0059] Step one, mix squalane, monolauric acid stearate and ethyl ferulate and heat to 90 DEG C to form an oil phase;

[0060] Step two, mix water and poloxamer 188 and heat to 80 DEG C to form an aqueous phase;

[0061] Step three, mix the aqueous phase into the oil phase and heat to 90 DEG C to form a mixed phase;

[0062] Step four, homogenize the mixed phase using a homogenizer at 13000 rpm for 8 min to form a mixed phase emulsion.

[0063] Step five, add phenoxyethanol to the mixed phase emulsion and use an ultrasonic instrument to perform ultrasonic treatment on the mixed phase emulsion at 90% power (maximum power 300 W) for 15 min to obtain a squalane-based nanolipid carrier loaded with ethyl ferulate.

[0064] The particle size and dispersion index of the squalane-based nanolipid carrier loaded with ethyl ferulate obtained in this example are shown in Table 1.

[0065] Example 3

[0066] A squalane-based nanolipid carrier loaded with ethyl ferulate, comprising the following components by weight percentage: squalane 8%; monoglyceride 8%; ethyl ferulate 0.5%; poloxamer 407 5.5%; phenoxyethanol 0.8%; and the balance being deionized water.

[0067] A squalane-based nanolipid carrier loaded with ethyl ferulate, comprising the following specific steps:

[0068] Step one, mix squalane, monoglyceride and ethyl ferulate and heat to 95 DEG C to form an oil phase;

[0069] Step two, mix water and poloxamer 407 and heat to 80 DEG C to form an aqueous phase;

[0070] Step three, mix the aqueous phase into the oil phase and heat to 90 DEG C to form a mixed phase;

[0071] Step four, the mixed phase was homogenized by homogenizer at 12000 rpm for 9 min to form the mixed phase emulsion.

[0072] Step five, phenoxyethanol was added into the mixed phase emulsion, and the mixed phase emulsion was ultrasonicated by ultrasonic instrument at 90% power (maximum power 300 W) for 20 min to obtain the squalane-based nanolipid carrier loaded with ethyl ferulate.

[0073] The particle size and dispersion index of the squalane-based nanolipid carrier loaded with ethyl ferulate obtained in the example are shown in Table 1.

[0074] Comparative Example 1

[0075] A squalane simple emulsion loaded with ethyl ferulate, which is different from Example 1 in that the preparation method step four is stirring at 420 rpm for 20 min.

[0076] Comparative Example 2

[0077] A squalane-based nanolipid carrier loaded with ethyl ferulate, which is different from Example 1 in that the solid oil is glycerol tristearate.

[0078] Comparative Example 3

[0079] A squalane-based nanolipid carrier, which is different from Example 1 in that ethyl ferulate is not added in the preparation process.

[0080] Comparative Example 4

[0081] A squalane-based nanolipid carrier loaded with ethyl ferulate, which is different from Example 1 in that the solid oil is ethylene glycol monostearate.

[0082] Comparative Example 5

[0083] A squalane-based nanolipid carrier loaded with ethyl ferulate, which is different from Example 1 in that the solid oil is myristic acid.

[0084] Comparative Example 6

[0085] A squalane-based nanolipid carrier loaded with ethyl ferulate, which is different from Example 1 in that the emulsifier is Span-60.

[0086] Comparative Example 7

[0087] A squalane-based nanolipid carrier loaded with ethyl ferulate, which is different from Example 1 in that the emulsifier is Span-80.

[0088] Test Example 1

[0089] Particle size, dispersion index and encapsulation efficiency test

[0090] (1) Experimental sample: ethyl ferulate-loaded squalane-based nanolipid carriers prepared in Examples 1-3 and Comparative Example.

[0091] (2) Experimental method: An appropriate amount of ethyl ferulate-loaded squalane-based nanolipid carriers was taken into an ultrafiltration centrifuge tube, centrifuged at 10,000 rpm for 30 min until no liquid remained in the ultrafiltration centrifuge tube, the inner centrifuge tube was taken, and an appropriate amount of ethanol was added, ultrasonic was performed for 20 min to break the liposome, and the content of ethyl ferulate was determined by ultraviolet spectrophotometer. Each sample was tested 3 times, and the average value was taken. The encapsulation efficiency was calculated by the following formula:

[0092]

[0093] The particle size and dispersion index of the product were directly determined at room temperature using Malven Zetasizer ULTRA.

[0094] (3) Experimental results: The experimental results are shown in Table 1.

[0095] Table 1: Encapsulation efficiency determination and related parameter determination results

[0096]

[0097]

[0098] As can be seen from Table 1, the ethyl ferulate-loaded squalane-based nanolipid carriers provided by the present application have an encapsulation efficiency of more than 90%, which has excellent encapsulation efficiency. The overall liposome particle size is less than 200 nm, which is in the small size range, which is beneficial to improve the encapsulation efficiency and skin permeability. The particle size measured in Example 1 is only 117 nm. The dispersion index of Examples 1, 2 and 3 is less than 0.3, which indicates that the ethyl ferulate-loaded squalane-based nanolipid carriers prepared have not only small particle size, but also very uniform dispersion.

[0099] The liposome particle sizes prepared using different solid oils are quite different. Suitable solid oils themselves have certain emulsifying properties, so they can be compounded with nonionic surfactants, which can further disperse the liposomes well. Other oils cannot be compounded with squalane and emulsifiers, so that liposomes with large particle size and large dispersion coefficient are prepared, which are more uneven and cannot achieve excellent particle size below 200 nm.

[0100] Test Example 2

[0101] Physical stability test

[0102] (1) Experimental sample: squalane-based nanolipid carriers loaded with ethyl ferulate prepared in Example 1, Example 2 and Example 3.

[0103] (2) Experimental method: The squalane-based nanolipid carriers loaded with ethyl ferulate prepared in Example 1, Example 2 and Example 3 were respectively stored at 4°C and 40°C for 24 hours, and centrifuged at 8000 rpm for 20 minutes. The samples were observed for whether they were layered or precipitated, and the particle size and dispersion index were determined.

[0104] (3) Experimental results: The experimental results are shown in Table 2.

[0105] Table 2

[0106]

[0107] As can be seen from Table 2, the particle size and dispersion index of the squalane-based nanolipid carriers loaded with ethyl ferulate prepared in Example 1, Example 2 and Example 3 did not change significantly after being stored at 4°C and 40°C for 24 hours and centrifuged at 8000 rpm for 20 minutes, and no layering or precipitation was found after centrifugation.

[0108] Test Example 3

[0109] In vitro transdermal rate test

[0110] (1) Experimental sample: squalane-based nanolipid carriers loaded with ethyl ferulate prepared in Example 1, squalane simple emulsion loaded with ethyl ferulate prepared in Comparative Example 1 and squalane-based nanolipid carriers loaded with ethyl ferulate prepared in Comparative Example 2.

[0111] (2) Experimental method: Nitrocellulose microporous filter membranes were soaked in isopropyl myristate for 20 minutes, removed, and gently squeezed with a water-absorbing paper to remove excess liquid. The prepared nitrocellulose microporous filter membranes were used. Turn on the switch of the drug transdermal diffusion instrument, and adjust the temperature of the diffusion cell to 32.5°C. Take two diffusion receiving cells, and fill them with 8 mL of normal saline as receiving liquid. Fix the prepared nitrocellulose microporous filter membranes between the supply cell and the receiving cell, and make them fully contact with the receiving liquid, and place them in the constant temperature water bath for heating. Take 1 mL of products of Example 1, Comparative Example 1 and Comparative Example 2 respectively and place them in the supply cell, and seal it. Turn on the magnetic stirrer and start timing, and take 100 μL of sample at different time points, and dilute it with anhydrous ethanol, and determine the content by ultraviolet-visible spectrophotometer, and supplement with an equal volume of constant temperature normal saline in time. After 48 hours of reaction.

[0112] (3) Experimental results: The experimental results are shown in Table 3. Figure 2

[0113] From Figure 2It can be concluded that the transdermal effects of the ethyl ferulate-loaded squalane nanolipid carriers prepared in Example 1 and Comparative Example 2 are much higher than those of the ethyl ferulate-loaded squalane emulsion prepared in Comparative Example 1 using the same raw materials. This is due to the small particle size of 100-200 nm of the ethyl ferulate-loaded squalane nanolipid carriers, which can penetrate the skin better than ordinary emulsions, allowing the skin to absorb nutrients and thus exert better efficacy.

[0114] Test Example 4

[0115] UV absorption peak test

[0116] (1) Experimental samples: ethyl ferulate-loaded squalane nanolipid carrier prepared in Example 1, and ethyl ferulate-loaded squalane nanolipid carrier prepared in Comparative Example 3.

[0117] (2) Experimental Method: 20 mg of the samples of Example 1 and Comparative Example 3 and 100 μg of ethyl ferulate were added to 20 mL of ethanol to the final volume and completely dissolved by sonication. Appropriate amounts of each sample were measured for absorption peaks using a UV-visible spectrophotometer (UV-1780, Shimadzu, Japan).

[0118] (3) Experimental results: The experimental results are shown in Figure 3

[0119] from Figure 3 It can be seen that the UV absorption peak of Example 1 in the 200-250 nm band is enhanced due to the combination of squalane and ethyl ferulate, which is much stronger than the absorption peak of ethyl ferulate and Control Example 3. This is due to the good compatibility of squalane and the interaction with ethyl ferulate to form a new liposome. On the one hand, squalane liposomes can provide good protection for ethyl ferulate, allowing the two to produce a superimposed effect of UV absorption capacity. On the other hand, the nano-scale particle size allows ethyl ferulate and squalane to be dispersed more evenly and cover a larger surface area, thereby providing better UV protection.

[0120] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, any equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical concepts disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. Squalane-based nanolipidic carriers loaded with ethyl ferulate, characterized in that, Comprise the following components by weight percentage: Squalane 4%~8%; Solid oil 4%~8%; Ethyl ferulate 0.1%~1.2%; Non-ionic surfactant 1.5%~5.5%; Alcohol solvent 0.3%~0.8%; The balance is deionized water; The solid oil is selected from any one of succinylated monostearate, monolaurate stearate, cetyl alcohol, monoglyceride, glycerol bis-stearate and glycerol behenate; The non-ionic surfactant is selected from any one of Tween-20, poloxamer 188, poloxamer 407, Tween-40 and Span 20; The alcohol solvent is selected from any one of phenoxyethanol, benzyl alcohol and cinnamyl alcohol; The particle size of the squalane-based nanolipid carrier loaded with ethyl ferulate is 100~200 nm; The embedding rate of ethyl ferulate in the squalane-based nanolipid carrier loaded with ethyl ferulate is greater than 90%; The preparation of the squalane-based nanolipid carrier loaded with ethyl ferulate comprises the following steps: (1) Mix squalane, solid oil and ethyl ferulate and heat at constant temperature to form an oil phase; (2) Mix deionized water and non-ionic surfactant and heat at constant temperature to form an aqueous phase; (3) Mix the aqueous phase into the oil phase and heat at constant temperature to form a mixed phase; (4) Homogenize the mixed phase by using a homogenizer at a speed of 9000~15000 rpm for 5~10 min to form a mixed phase emulsion; (5) Add alcohol solvent to the mixed phase emulsion and perform ultrasonic treatment on the mixed phase emulsion by using an ultrasonic instrument to obtain the squalane-based nanolipid carrier loaded with ethyl ferulate.

2. The squalane-based ethyl ferulate loaded nano-lipidic carrier according to claim 1, characterized in that, The dispersion index of the squalane-based nanolipid carrier loaded with ethyl ferulate is 0.22-0.

26.

3. Process for the preparation of ethyl ferulate loaded squalane-based nanolipid carriers according to any one of claims 1-2, characterized in that, Comprise the following steps: (1) Mix squalane, solid oil and ethyl ferulate and heat at constant temperature to form an oil phase; (2) Mix deionized water and non-ionic surfactant and heat at constant temperature to form an aqueous phase; (3) Mix the aqueous phase into the oil phase and heat at constant temperature to form a mixed phase; (4) Homogenize the mixed phase by using a homogenizer at a speed of 9000~15000 rpm for 5~10 min to form a mixed phase emulsion; (5) Add alcohol solvent to the mixed phase emulsion and perform ultrasonic treatment on the mixed phase emulsion by using an ultrasonic instrument to obtain the squalane-based nanolipid carrier loaded with ethyl ferulate.

4. The process for the preparation of ethyl ferulate loaded squalane based nano-lipidic carrier according to claim 3, wherein, The temperature of constant heating in step (1) is 90~95℃; The temperature of constant heating in step (2) is 75~80℃; The temperature of constant heating in step (3) is 90~95℃; The power of ultrasonic treatment in step (5) is 240-300W for 10~20 min.

5. The squalane-based nanolipid carrier loaded with ethyl ferulate according to claim 1 or 2 is used in the preparation of skin care products.

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