A high mountain plant composition for nourishing skin, preventing hair loss and strengthening hair, its essence and preparation method

Abstract

The present invention discloses a high mountain plant composition for nourishing skin, preventing hair loss and strengthening hair, and an essence thereof, and a preparation method thereof, aiming to provide a high mountain plant composition for nourishing and repairing the head skin, reducing hair loss, and hair care, and an essence thereof; the technical solution includes: 6.25-35% of Salvia miltiorrhiza Bunge extract, 3.75-15% of Lamium album L. flower extract, 12-36% of Eclipta prostrata L. leaf extract, 8%-24% of Platycladus orientalis (L.) Franco leaf extract, and 30%-70% of Gentiana scabra Bunge root extract; it belongs to the daily chemical technology field.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily chemicals, and particularly relates to a nourishing skin and preventing hair loss and promoting hair growth essence containing alpine plant extracts. Background Art

[0002] As the main physiological attachment of hair, the physiological state of the scalp will significantly affect the hair state. Insufficient nutrition of the scalp will lead to insufficient nutrition of hair follicles, further leading to problems such as accelerated hair loss, hair splitting, and hair breakage. In addition, the sebum secretion amount of the scalp is significantly higher than that of facial skin. The rapid secretion of oil will lead to the growth of Malassezia, further leading to problems such as peculiar smell, dandruff, and itchy inflammation. At the same time, the scalp ages faster than facial and body skin. The aging speed of the scalp is 6 times that of facial skin and 12 times that of body skin. The metabolic cycle of facial skin is 28 days, while the cell metabolic cycle of the scalp is only 14 - 21 days. The aging of the scalp leads to the aging of scalp follicle cells, resulting in more scalp problems, such as dandruff, oily scalp, itchy scalp, inflammation, and hair loss.

[0003] Regarding the problem of scalp dandruff, there is much research in the current industry. Most use anti-dandruff shampoos for deep cleaning to timely remove excessive oil secretion, inhibit the overgrowth of Malassezia, and reduce scalp peculiar smell. However, there is no good solution for problems such as scalp nutrition and scalp aging. In traditional ancient prescriptions, materials obtained by decocting and filtering traditional Chinese medicines such as mulberry bark and biota orientalis leaves are used for washing hair or soaking. The color and smell are unacceptable, it is inconvenient to use, the compliance of the target population is poor, and the effect cannot be confirmed. Some plant medicinal materials are simply stacked without targeted combination, resulting in poor extraction effect; there are problems such as high cost, poor safety, and large side effects of active substances.

[0004] Therefore, in view of this problem, in-depth research on solving excessive hair loss, hair breakage, etc. caused by internal factors such as insufficient scalp nutrition, cell aging, and reduced hair follicle vitality by nourishing the skin has great technical value and economic value. Summary of the Invention

[0005] In view of the defects existing in the prior art, the purpose of the present invention is to provide a nourishing and repairing scalp, reducing hair loss, and hair care nourishing skin and preventing hair loss and promoting hair growth alpine plant composition.

[0006] The second purpose of the present invention is to provide the above-mentioned essence for nourishing skin and preventing hair loss and promoting hair growth containing alpine plant extracts.

[0007] The second purpose of the present invention is to provide a preparation method of the essence for nourishing skin and preventing hair loss and promoting hair growth with alpine plant extracts.

[0008] To solve the above technical problems, the first technical solution adopted by the present invention is as follows:

[0009] A high mountain plant composition for nourishing skin, preventing hair loss and strengthening hair roots, comprising components in the following weight ratios: 6.25 - 35% of Salvia miltiorrhiza Bunge extract, 3.75 - 15% of Lamium album L. flower extract, 12 - 36% of Eclipta prostrata L. leaf extract, 8% - 24% of Platycladus orientalis (L.) Franco leaf extract, 30% - 70% of Gentiana scabra Bunge root extract;

[0010] Further, the above high mountain plant composition for nourishing skin, preventing hair loss and strengthening hair roots is characterized in that the Salvia miltiorrhiza Bunge extract, Lamium album L. flower extract and Eclipta prostrata L. leaf extract are prepared by the following steps:

[0011] Weigh the raw medicinal materials of Salvia miltiorrhiza Bunge, Lamium album L. and Eclipta prostrata L., cut them into sections and crush them into powders of 40 - 60 meshes, add 10 - 20 times of the material - liquid ratio of pure water at 50°C into the powders, stir for 5 min, keep them soaking at 50°C for 30 min, add 0.1 - 0.5% of compound enzyme based on the content of the medicinal materials, and carry out extraction through a hydraulic cavitation device. The extraction parameters are: the temperature of the material - liquid is 50°C, the outlet pressure is 0.2 MPA, and circulate for 6 times. Then, carry out extraction through the hydraulic cavitation device again, and the treatment parameters are that the temperature of the material - liquid is 50°C, the outlet pressure is 0.2 MPa, and circulate for 5 times. Heat up to 90°C to inactivate the enzyme and then discharge the material. Collect the material - liquid, remove the solid medicinal residues to obtain the Salvia miltiorrhiza Bunge extract, Lamium album L. flower extract and Eclipta prostrata L. leaf extract.

[0012] Further, for the above high mountain plant composition for nourishing skin, preventing hair loss and strengthening hair roots, the compound enzyme is composed of cellulase, hemicellulase and pectinase according to the mass ratio of 1:0.5:0.2.

[0013] The second technical solution provided by the present invention is as follows:

[0014] An essence containing the above high mountain plant composition for nourishing skin, preventing hair loss and strengthening hair roots, comprising components in the following weight parts:

[0015] Phase A: 36.86 - 76.81 parts of water, 0.03 - 0.08 parts of disodium EDTA, 0.05 - 0.15 parts of allantoin;

[0016] Phase B: 0.1 - 0.5 parts of hydroxyethyl cellulose, 1 - 3 parts of glycerol;

[0017] Phase C: 0.03 - 0.08 parts of menthol, 1 - 5 parts of ethanol;

[0018] Phase D: 0.1 - 0.3 parts of essence, 0.2 - 0.6 parts of phenoxyethanol, 2 - 6 parts of propylene glycol, 0.3 - 0.9 parts of PEG - 40 hydrogenated castor oil;

[0019] Phase E: 2 - 8 parts of deep - fat inhibitor, 2 - 8 parts of hydrogenated starch hydrolysate, 2 - 8 parts of Runsoft PGP, 1 - 30 parts of alpine plant composition for nourishing skin, preventing hair loss and strengthening hair roots; 0.2 - 0.6 parts of sodium benzoate;

[0020] Phase F: 1 - 2 parts of water, 0.03 - 0.08 parts of citric acid.

[0021] Furthermore, for the essence containing the alpine plant composition for nourishing skin, preventing hair loss and strengthening hair roots mentioned above, the deep - fat inhibitor is composed of butanediol and 10 - hydroxydecanoic acid.

[0022] Furthermore, for the essence containing the alpine plant composition for nourishing skin, preventing hair loss and strengthening hair roots mentioned above, the Runsoft PGP is sodium cocamidopropyl PG - dimonium chloride phosphate and sodium chloride.

[0023] The third technical solution provided by the present invention is the preparation method of the essence containing the alpine plant composition for nourishing skin, preventing hair loss and strengthening hair roots, which successively includes the following steps:

[0024] 1) Add A to the emulsifying pot, heat it to 70 - 75°C, then add the uniformly dispersed component B, and stir to dissolve it into a transparent liquid;

[0025] 2) Keep stirring, continue to cool down to 40 - 45°C, successively add the components of Phase C, Phase D, Phase E and Phase F, stir for 10 - 15 minutes, adjust the pH to 5.5 - 6.0, and after the material is uniformly mixed, it can be filtered and discharged.

[0026] Furthermore, for the preparation method of the essence containing the alpine plant composition for nourishing skin, preventing hair loss and strengthening hair roots mentioned above, it successively includes the following steps:

[0027] 1) Prepare Phase B in advance: Mix and disperse it uniformly in advance for standby;

[0028] 2) Phase A: Heat it to 70 - 75°C, add the uniformly dispersed component B of Phase B, and stir to dissolve it into a transparent liquid. During the process, it can be homogenized appropriately until the material is transparent and free of particulate matter;

[0029] 3) Prepare Phase C in advance: Place menthol in ethanol and heat to dissolve menthol completely;

[0030] 4) Prepare Phase D in advance: Mix the essence and PEG - 40 hydrogenated castor oil and stir for 3 minutes, then add phenoxyethanol and propylene glycol, and continue to mix and stir for 5 minutes until the material is uniformly mixed into a transparent liquid;

[0031] 5) Keep stirring, continue to cool down to 40 - 45°C, successively add the components of Phase C, Phase D and Phase E, and stir for 10 - 15 minutes until the material is uniform;

[0032] 6) Preparation of Phase F: Dissolve citric acid in water to form a transparent liquid;

[0033] 7) Add the material of Phase F and stir for 10 - 15 minutes, adjust the pH to 5.5 - 6.0;

[0034] 8) Take samples to detect various physical and chemical indexes. After passing the inspection, filter and discharge the material.

[0035] Compared with the prior art, the present invention has the following technical advantages:

[0036] 1. The technical solution provided by the present invention uses the said Salvia miltiorrhiza Bunge extract and Lamium barbatum Sieb. extract, which can activate blood circulation to moisten the skin, nourish the skin, enhance hair follicle nutrition, and reduce hair loss; compounded with Eclipta prostrata L. leaf extract and Platycladus orientalis (L.) Franco extract to stimulate hair growth, promote the conversion of white hair to black hair, and slow down hair loss; further compounded with Gentiana scabra Bunge root extract to repair scalp inflammation and trauma, enhance skin vitality, and the skin feel regulator controls oil, relieves itching, lubricates, etc., enhancing the soft, smooth and refreshing skin feel during use, and reducing the sticky and uncomfortable feeling.

[0037] 2. The technical solution provided by the present invention adopts the hydrodynamic cavitation extraction technology, effectively solving the adverse reaction problems such as poor extraction effect of Salvia miltiorrhiza Bunge in the prior art, poor activity, poor safety, and large side effects;

[0038] 3. The scalp essence provided by the present invention uses the alpine plants in the Yunnan - Guizhou Plateau as the main key components. Through research, multiple solutions such as promoting scalp blood flow, increasing skin nutrition, stimulating the growth of hair papilla cells, scavenging cell free radicals, and inhibiting inflammation work together to delay cell aging, nourish scalp skin, increase hair follicle vitality, and thus solve problems related to excessive hair loss, hair breakage, etc. And it designs to solve the difficulties in daily use of the product, strengthen the convenience of product use, and increase the acceptance degree of the target population. Specific Embodiments

[0039] To better illustrate the purpose, technical solution and technical effect of the present invention, the present invention will be further described below with specific embodiments.

[0040] The following are adopted:

[0041] The Platycladus orientalis (L.) Franco extract is purchased from Shanghai Boshuo Industrial Co., Ltd.;

[0042] The Gentiana scabra Bunge root extract is prepared with reference to the preparation method of CN201910929044.5, an anti - allergic and anti - itching plant composition and its preparation method and application.

[0043] Example 1

[0044] A nourishing skin, preventing hair loss and strengthening hair growth alpine plant composition provided by this embodiment is composed of the following components by mass percentage: Salvia miltiorrhiza Bunge extract 6.25%, Lamium barbatum Sieb. extract 3.75%, Eclipta prostrata L. leaf extract 36%, Platycladus orientalis (L.) Franco leaf extract 24%, Gentiana scabra Bunge root extract 30%.

[0045] Example 2

[0046] A nourishing skin, preventing hair loss and strengthening hair growth alpine plant composition provided by this embodiment is composed of the following components by mass percentage: Salvia miltiorrhiza Bunge extract 20%, Lamium barbatum Sieb. extract 10%, Eclipta prostrata L. leaf extract 12%, Platycladus orientalis (L.) Franco leaf extract 8%, Gentiana scabra Bunge root extract 50%.

[0047] Example 3

[0048] A nourishing skin, preventing hair loss and strengthening hair growth alpine plant composition provided by this embodiment is composed of the following components by mass percentage: Salvia miltiorrhiza Bunge extract 13%, Lamium barbatum Sieb. extract 7%, Eclipta prostrata L. leaf extract 25%, Platycladus orientalis (L.) Franco leaf extract 15%, Gentiana scabra Bunge root extract 40%.

[0049] Example 4

[0050] A nourishing skin, preventing hair loss and strengthening hair growth alpine plant composition provided by this embodiment is composed of the following components by mass percentage: Salvia miltiorrhiza Bunge extract 12%, Lamium barbatum Sieb. extract 8%, Eclipta prostrata L. leaf extract 12%, Platycladus orientalis (L.) Franco leaf extract 8%, Gentiana scabra Bunge root extract 60%.

[0051] The Salvia miltiorrhiza Bunge extract, Lamium barbatum Sieb. extract and Eclipta prostrata L. leaf extract described in Examples 1 to 4 are prepared by the following method:

[0052] Weigh the raw medicinal materials Salvia miltiorrhiza Bunge, Lamium barbatum Sieb. and Eclipta prostrata L., cut them into sections and then crush them into 40-60 mesh powder. Add 10-20 times of pure water at 50°C according to the material-liquid ratio into the powder, stir for 5 min, keep it warm and soak for 30 min at 50°C, add 0.1-0.5% of compound enzyme based on the content of the medicinal materials (the compound enzyme is composed of cellulase, hemicellulase and pectinase according to the mass ratio of 1:0.5:0.2), and pass it through a hydraulic cavitation device for extraction. The extraction parameters are: material-liquid temperature 50°C, outlet pressure 0.2 MPA, circulate 6 times, then pass it through the hydraulic cavitation device for extraction again, and the treatment parameters are material-liquid temperature 50°C, outlet pressure 0.2 MPa, circulate 5 times, raise the temperature to 90°C to inactivate the enzyme and then discharge the material, collect the material liquid, remove the solid medicinal residues, and obtain the Salvia miltiorrhiza Bunge extract. Further, the above-mentioned nourishing skin, preventing hair loss and strengthening hair growth alpine plant composition.

[0053] Example 5

[0054] This embodiment provides a nourishing skin, preventing hair loss and strengthening hair growth essence containing alpine plant extracts, including the following components by weight:

[0055] Phase A: 51.47 parts of water, 0.05 part of disodium EDTA, 0.1 part of allantoin;

[0056] Phase B: 0.5 part of hydroxyethyl cellulose, 2 parts of glycerol;

[0057] Phase C: 0.05 part of menthol, 4 parts of ethanol;

[0058] Phase D: 0.2 part of essence, 0.4 part of phenoxyethanol, 4 parts of propylene glycol, 0.5 part of PEG - 40 hydrogenated castor oil;

[0059] Phase E: 5 parts of deep - layer fat inhibitor, 4 parts of hydrogenated starch hydrolysate, 6 parts of Runsoft PGP, 20 parts of the nourishing skin, anti - hair - loss and hair - strengthening alpine plant composition prepared in Example 2; 0.2 part of sodium benzoate;

[0060] Phase F: 1.5 parts of water, 0.03 part of citric acid.

[0061] Example 6

[0062] This example provides an essence for nourishing skin, preventing hair loss and strengthening hair containing alpine plant extracts, comprising the following components in parts by weight:

[0063] Phase A: 76.81 parts of water, 0.03 part of disodium EDTA, 0.15 part of allantoin;

[0064] Phase B: 0.1 part of hydroxyethyl cellulose, 3 parts of glycerol;

[0065] Phase C: 0.08 part of menthol, 1 part of ethanol;

[0066] Phase D: 0.1 part of essence, 0.6 part of phenoxyethanol, 2 parts of propylene glycol, 0.9 part of PEG - 40 hydrogenated castor oil;

[0067] Phase E: 2 parts of deep - layer fat inhibitor, 8 parts of hydrogenated starch hydrolysate, 2 parts of Runsoft PGP, 5 parts of the nourishing skin, anti - hair - loss and hair - strengthening alpine plant composition prepared in Example 2; 0.2 part of sodium benzoate;

[0068] Phase F: 2 parts of water, 0.03 part of citric acid.

[0069] Example 7

[0070] This example provides an essence for nourishing skin, preventing hair loss and strengthening hair containing alpine plant extracts, comprising the following components in parts by weight:

[0071] Phase A: 36.86 parts of water, 0.08 part of disodium EDTA, 0.05 part of allantoin;

[0072] Phase B: 0.5 part of hydroxyethyl cellulose, 1 part of glycerol;

[0073] Phase C: 0.03 parts of menthol, 5 parts of ethanol;

[0074] Phase D: 0.3 parts of essence, 0.2 parts of phenoxyethanol, 6 parts of propylene glycol, 0.3 parts of PEG - 40 hydrogenated castor oil;

[0075] Phase E: 8 parts of deep - fat inhibitor, 2 parts of hydrogenated starch hydrolysate, 8 parts of Runsoft PGP, 30 parts of the nourishing skin and preventing hair loss alpine plant composition prepared in Example 2; 0.6 parts of sodium benzoate;

[0076] Phase F: 1 part of water, 0.08 parts of citric acid.

[0077] Comparative Example 1

[0078] It is composed of Salvia miltiorrhiza Bunge extract and Lamium barbatum Sieb. extract, and their mass ratio is: 5:3.

[0079] Comparative Example 2

[0080] It is composed of Eclipta prostrata L. leaf extract and Platycladus orientalis (L.) Franco leaf extract, and their mass ratio is: 3:2.

[0081] Comparative Example 3

[0082] Its components are the same as those in Example 1, the difference is that the Salvia miltiorrhiza Bunge extract, Lamium barbatum Sieb. extract and Eclipta prostrata L. leaf extract are prepared by the following method:

[0083] Weigh the raw materials of Salvia miltiorrhiza Bunge, Lamium barbatum Sieb. and Eclipta prostrata L., cut them into sections and then crush them into 40 - 60 - mesh powder. Add 10 - 20 times of the material - liquid ratio of pure water at 50°C into the powder, stir for 5 min, keep it warm and soak for 30 min at 50°C, then pass it through a hydrodynamic cavitation device for extraction. The extraction parameters are: the temperature of the material - liquid is 50°C, the outlet pressure is 0.2 MPA, and cycle 6 times. Then pass it through the hydrodynamic cavitation device for extraction again, and the treatment parameters are the temperature of the material - liquid is 50°C, the outlet pressure is 0.2 MPa, and cycle 5 times. Heat it up to 90°C to inactivate enzymes and then discharge, collect the material - liquid, remove the solid medicinal residues, and obtain the Salvia miltiorrhiza Bunge extract, Lamium barbatum Sieb. extract and Eclipta prostrata L. leaf extract.

[0084] Comparative Example 4

[0085] It only contains Salvia miltiorrhiza Bunge extract.

[0086] Comparative Example 5

[0087] It only contains Lamium barbatum Sieb. extract.

[0088] Comparative Example 6

[0089] It only contains Eclipta prostrata L. leaf extract.

[0090] Comparative Example 7

[0091] Contains only Platycladus orientalis leaf extract.

[0092] Comparative Example 8

[0093] Contains only Gentiana root extract.

[0094] Comparative Example 9

[0095] Other components are the same as in Example 5, except that hydrogenated starch hydrolysate is used instead of sodium cocoamido propyl PG-dimethyl ammonium chloride phosphate.

[0096] Comparative Example 10 Essence

[0097] Other components are the same as in Example 5, except that the nourishing and hair loss-preventing alpine plant composition of the present application is missing.

[0098] In order to prove the technical advantages of the technical solution provided by the present application, the following detection experiments of the technical solution provided by the present application are given:

[0099] 1. 5α-Reductase Inhibition Test:

[0100] Dihydrotestosterone (DHT), a metabolite of testosterone, plays an important role in androgenetic alopecia. The levels of dihydrotestosterone and the quantity and activity of 5α-reductase in the scalp and serum of patients with androgenetic alopecia are higher than those in the normal population, and the ability of testosterone to be converted into dihydrotestosterone in their hair follicles also increases. Since 5α-reductase in the hair follicles mediates the conversion of testosterone into dihydrotestosterone, 5α-reductase inhibitors such as finasteride can prolong the growth phase of hair follicles, prevent hair loss and promote hair growth. The anti-hair loss efficacy of the test substance is evaluated through the 5α-reductase inhibition test.

[0101] The 5α-reductase inhibition effect of the sample is detected by a 5α-reductase ELISA kit, and the results are shown in Table 1:

[0102]

[0103] Result analysis: It can be known from experimental tests that when the examples and comparative examples are diluted at the same gradient, after diluting 500 times, the inhibitory effect of the example sample on 5α-reductase is better than that of the positive substance (finasteride 4 mmol / L). Through the single extract test and analysis, it can be known that the extract that mainly inhibits the effect of 5α-reductase is the B component. Among them, the Platycladus orientalis leaf extract in Comparative Example 7 has the best effect among all single extracts. The combination of Comparative Example 6 and Comparative Example 7 to form Comparative Example 2 shows that the inhibitory effect is further enhanced, reaching 73%. Further, on the basis of the B component, the ratio is optimized, and the inhibitory effect is better after adding Salvia miltiorrhiza extract, Lamium album flower extract and Gentiana root extract, and the inhibitory effect reaches more than 80%. Compared with Example 1, although the extract ratio of Comparative Example 3 is the same, due to certain differences in the process, the inhibitory effect on enzyme activity differs by 27%, indicating that enzymatic hydrolysis has an obvious effect on further releasing the active ingredients in plant cells and enhancing plant activity. The inhibitory rates of Examples 1-4 on 5α-reductase are nearly twice as good as those of the positive group, indicating that they have a good effect on hair loss caused by the overexpression of 5α-reductase.

[0104] 2. In this experiment, dermal papilla cells were used as the experimental object, and an in vitro cell model of androgenetic alopecia was established by stimulating with a certain concentration of dihydrotestosterone. Then, the in vitro model of androgenetic alopecia was treated with the test active substance, and the efficacy of the test active substance in promoting hair regeneration was evaluated by detecting the changes in the secretion factors VEGF, DKK-1, TGFβ2 and the contents of β-catenin and AR in dermal papilla cells.

[0105] Dermal papilla cells secrete some factors that promote or inhibit the growth of epidermal cells in the hair follicle. Typical factors mainly include growth factor-like secretion factors VEGF, DKK-1, TGF-β2 and β-catenin, AR. The secretion content of VEGF was detected by ELISA method, and the contents of DKK-1, TGF-β2, β-catenin and AR were detected by RT-PCR method. Among them, AR is the regulation of androgen receptor, and DKK-1, TGF-β2 and β-catenin are cell proliferation and apoptosis factors; VEGF is a cell growth factor. The results are shown in Table 2.

[0106]

[0107] Note: The products provided in Examples 1 to 4 are sequentially recorded as: 1#, 2#, 3#, 4#;

[0108] The products provided in Comparative Examples 1-8 are sequentially recorded as: D1#, D2#, D3#, D4#, D5#, D6#, D7#, D8#.

[0109] After testing the test samples by the MTT assay, a concentration of 0.1% was preferably used for cell testing. After RT-PCR amplification, the change amounts of various factors were analyzed. It can be seen that, compared with the blank group, the examples had a significant increasing effect on the expression of the VEGF factor. The highest was Example 1, reaching 89.23%, indicating that it could promote the growth of dermal papilla cells. Further comparative analysis found that among the single extracts, Comparative Example 7 had the best promoting effect, reaching 65.23%. After compounding Comparative Example 6 and Comparative Example 7, Comparative Example 2 stimulated the further increase of the VEGF factor to 75.25%, indicating that Component B was the key factor for promoting hair growth in this composition.

[0110] Analysis of the inhibition rates of DKK-1, TGF-β2, and β-catenin that cause apoptosis of dermal papilla cells found that Examples 1-4 had good inhibitory effects on all three, indicating that they had good inhibitory effects on the three apoptotic factors and could effectively inhibit the apoptosis of dermal papilla cells and avoid premature hair aging and shedding. Further analysis found that among the single extracts, the Salvia miltiorrhiza Bunge extract in Comparative Example 4 had the highest inhibition rate of DKK-1, reaching 54.20%, and the Lamium album L. flower extract in Comparative Example 5 had the highest inhibition rate of β-catenin, reaching 72.20%, which was the best in the single extracts. After combining Comparative Example 4 and 5 to obtain Comparative Example 1, it was found that there was a synergistic effect in the inhibition rates of the three apoptotic factors. Through the analysis of the cell apoptosis factors, it was found that Component A was the key component mainly inhibiting cell apoptosis. The examples obtained by synergistically combining the three components A, B, and C all showed better inhibitory effects, indicating that the examples could inhibit the apoptosis of dermal papilla at the cell level.

[0111] In addition, compared with Example 1, Comparative Example 3 had a certain decrease in the regulatory effects on various growth factors, with a range of 10-30%, further verifying the key value of the enzymatic hydrolysis process for the extraction of active substances.

[0112] In summary, Component A and Component B had good performances in cell growth and cell apoptosis respectively. After the synergistic antioxidant effect of Component C, they showed the effects of maintaining hair growth and preventing hair loss at the cell level.

[0113] 3. Human trials (nourishing the scalp, reducing hair loss, hair care)

[0114] By screening 120 alopecia volunteers and having them sign the informed consent form for volunteers. The volunteers were randomly divided into two groups, with 30 people in each group. The first group of volunteers used the blank paste base group, the second group used the product of Example 5, the third group used the product of Comparative Example 10, and the fourth group used the product of Comparative Example 11. The products were used for 4 weeks, 8 weeks, and 12 weeks, and the overall hair density increase, hair loss count reduction, hair becoming more smooth, scalp nourishing effect, scalp moisturizing, etc. were scored. Among them, Table 3 is the hair density grading score table, and the test evaluation results are shown in Tables 4 - 6:

[0115] Note: In the following tables, the products provided in Example 5 are denoted as 5# in sequence;

[0116] The products provided in Comparative Examples 9 and 10 are denoted as: D9#, D10# in sequence.

[0117]

[0118] After 12 - week testing, it can be seen that there is no obvious change in the blank base group, and no one feels any change in hair loss and increase. Example 5 contains 20% of the components of Example 2, and Comparative Example 9 also contains 20% of Example 2, but lacks the skin conditioner cocamidopropyl PG - dimonium chloride phosphate sodium. Comparative Example 10 does not contain Example 2, but contains a skin conditioner. After 12 - week testing, it can be seen that in the Example 5 group, 7 people reported a reduction in hair loss, and 9 people had an increase in hair density. The effect is relatively obvious, and positive feedback was received for scalp nourishing, moisturizing, and hair repair effects, indicating that Example 5 has good nourishing, moisturizing, hair loss reduction, and hair quantity increase effects on alopecia volunteers. Moreover, the data became gradually obvious starting from the 4th week, was basically obvious at the 8th week, and the data basically did not increase at the 12th week.

[0119] Through data comparison and analysis, it can be seen that in Comparative Example 9, there was basically no change in anti - hair loss and hair growth promotion after the 4th week, and it was far lower than Example 5, about half the number of people in Example 5. However, it performed better in scalp conditioning, and the number of people with positive feedback was relatively close to that of Example 5; in Comparative Example 10, it was superior to Comparative Example 9 in anti - hair loss and hair growth promotion, but weaker than Example 5. However, it was poor in scalp conditioning, weaker than Example 5 and Comparative Example 9.

[0120] Based on the above analysis, it can be seen that Example 5 contains an alpine plant composition, which has good effects in reducing hair loss and increasing hair density, and also has significant effects in nourishing and moisturizing the scalp. Moreover, the skin conditioner also shows a certain synergistic effect in skin conditioning and repair.

[0121] The above are only the preferred embodiments of the present invention, but the present invention is not limited to the embodiments. Those skilled in the art can make equivalent deformations or substitutions according to the technical concept of the present invention within the scope disclosed by the present invention, and all are included in the protection scope of the present invention.

Claims

1. A high mountain plant composition for nourishing skin, preventing hair loss and strengthening hair roots, characterized in that, It contains components in the following weight ratios: Salvia przewalskii Maxim. extract 6.25 - 35%, Lamium barbatum Sieb. ex Spreng. flower extract 3.75 - 15%, Eclipta prostrata L. leaf extract 12 - 36%, Platycladus orientalis (L.) Franco leaf extract 8% - 24%, Gentiana scabra Bunge root extract 30% - 70%; The Salvia przewalskii Maxim. extract, Lamium barbatum Sieb. ex Spreng. flower extract, and Eclipta prostrata L. leaf extract are prepared through the following steps: Weigh the raw medicinal materials Salvia przewalskii Maxim., Lamium barbatum Sieb. ex Spreng., and Eclipta prostrata L. according to the weight ratio, cut them into sections and then crush them into powders of 40 - 60 mesh. Add pure water at 50°C at a material - liquid ratio of 10 - 20 times the material weight into the powders, stir for 5 min, keep them soaking at 50°C for 30 min, add 0.1 - 0.5% compound enzyme based on the content of the medicinal materials, and conduct extraction through a hydraulic cavitation device. The extraction parameters are: material - liquid temperature 50°C, outlet pressure 0.2 MPa, circulate 6 times. Then conduct extraction through the hydraulic cavitation device again, and the treatment parameters are material - liquid temperature 50°C, outlet pressure 0.2 MPa, circulate 5 times. Heat up to 90°C to inactivate the enzyme and then discharge the material. Collect the material liquid, remove the solid medicinal residues to obtain Salvia przewalskii Maxim. extract, Lamium barbatum Sieb. ex Spreng. flower extract, and Eclipta prostrata L. leaf extract.

2. The nourishing skin and preventing hair loss and strengthening hair growth alpine plant composition according to claim 1, wherein, The compound enzyme is composed of cellulase, hemicellulase, and pectinase in a mass ratio of 1:0.5:0.

2.

3. An essence containing the alpine plant composition for nourishing skin and preventing hair loss as claimed in claim 1, characterized in that, It includes components in the following weight parts: Phase A: water 36.86 - 76.81 parts, disodium EDTA 0.03 - 0.08 parts, allantoin 0.05 - 0.15 parts; Phase B: hydroxyethyl cellulose 0.1 - 0.5 parts, glycerol 1 - 3 parts; Phase C: menthol 0.03 - 0.08 parts, ethanol 1 - 5 parts; Phase D: essence 0.1 - 0.3 parts, phenoxyethanol 0.2 - 0.6 parts, propylene glycol 2 - 6 parts, PEG - 40 hydrogenated castor oil 0.3 - 0.9 parts; Phase E: deep - layer fat inhibitor 2 - 8 parts, hydrogenated starch hydrolysate 2 - 8 parts, RunsoftPGP 2 - 8 parts, nourishing skin, preventing hair loss and strengthening hair mountain plant composition 1 - 30 parts; sodium benzoate 0.2 - 0.6 parts; Phase F: water 1 - 2 parts, citric acid 0.03 - 0.08 parts; The RunsoftPGP is sodium cocamidopropyl PG - dimonium chloride phosphate and sodium chloride.

4. The essence according to claim 3, characterized in that, The deep - layer fat inhibitor is composed of butanediol and 10 - hydroxydecanoic acid.

5. The preparation method of the essence according to claim 3, characterized in that, It successively includes the following steps: 1) Add the components of Phase A to an emulsifying pan, heat to 70 - 75°C, and then add the uniformly dispersed components of Phase B, stir to dissolve them into a transparent liquid; 2) Keep stirring, continue to cool down to 40 - 45°C, successively add the components of Phase C, Phase D, Phase E, and Phase F, stir for 10 - 15 minutes, adjust the pH to 5.5 - 6.0, and after the material body is uniformly mixed, it can be filtered and discharged.

6. The preparation method of the essence according to claim 5, characterized in that, It successively includes the following steps: 1) Prepare Phase B in advance: pre - mix and disperse it evenly for standby; 2) Phase A: Heat to 70 - 75°C, add the uniformly dispersed Phase B, stir to dissolve it into a transparent liquid; 3) Prepare Phase C: Place menthol in ethanol and heat to completely dissolve menthol; 4) Preparation of Phase D: After mixing the fragrance with PEG-40 hydrogenated castor oil and stirring for 3 minutes, then add phenoxyethanol and propylene glycol, and continue to mix and stir for 5 minutes until the material is uniformly mixed into a transparent liquid; 5) Keep stirring and continue to cool down to 40 - 45 °C, successively add each component of Phase C, Phase D and Phase E, and stir for 10 - 15 minutes until the material is uniform; 6) Preparation of Phase F: Dissolve citric acid in water to form a transparent liquid; 7) Add the material of Phase F, stir for 10 - 15 minutes, and adjust the pH to 5.5 - 6.0; 8) Take samples to detect various physical and chemical indexes. After passing the inspection, filter and discharge the material.

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