A self-powered system and method for catalytic therapy

Abstract

This application discloses a self-powered system and method for catalytic therapy. The system includes: a triboelectric nanogenerator for generating alternating current pulses; a first electrode connected to one end of the triboelectric nanogenerator; and a second electrode connected to the other end of the triboelectric nanogenerator. A pulsed electric field exists between the first and second electrodes. A conductive hydrogel containing nanocatalysts and a physiological buffer solution containing cells are also placed within the system. When performing catalytic therapy on cells using the self-powered system provided in this application, the triboelectric nanogenerator generates an alternating current pulsed electric field. Under the influence of this electric field, the permeability of the cell membrane is enhanced, and a large amount of reactive oxygen species (ROS) are generated. The generated ROS enter the cells, thereby killing the cells and achieving the purpose of catalytic therapy, thus realizing electric field-enhanced catalytic therapy.

Description

Technical Field

[0001] This invention relates to the field of catalytic therapy technology, and in particular to a self-powered system and method for catalytic therapy. Background Technology

[0002] In the medical field, the generation of excess reactive oxygen species (ROS) has attracted widespread attention as a highly specific and non-invasive biological therapeutic approach. Related technologies often rely on nanocatalysts to improve the catalytic efficiency of ROS. However, many commercially available nanocatalysts suffer from low catalytic efficiency and unsatisfactory catalytic kinetics. The main reason for this is that the electron mobility in nanocatalysts significantly affects catalytic efficiency. Due to the high energy required for catalytic reactions, the electron mobility of the catalyst is limited, thus limiting its ability to interact with other substances. Applying an electric field can improve the catalytic activity of nanocatalysts to some extent. However, traditional electric field construction is achieved through an external power source, which limits the construction process due to external devices, complicating the treatment process. Summary of the Invention

[0003] This application provides a self-powered system and method for catalytic therapy. By generating an alternating pulsed electric field through a triboelectric nanogenerator, the permeability of the cell membrane is enhanced and a large amount of reactive oxygen molecules are generated, thereby killing cells and achieving the purpose of catalytic therapy, realizing catalytic therapy enhanced by electric field.

[0004] The technical solution provided in this application has at least the following advantages compared to the prior art:

[0005] Firstly, the embodiments of this application construct a self-driven electric field through a triboelectric nanogenerator to replace the traditional external power supply, enabling the entire system to achieve self-powered operation, miniaturization, cost savings, energy efficiency, and economic benefits. It also provides a pathway for wearable medical devices.

[0006] Secondly, traditional electrostimulation therapy mostly applies external stimulation by constructing a direct current electric field. However, the presence of a direct current electric field causes redox reactions between cations and anions at the positive and negative electrodes, leading to drastic changes in the pH value around the electrodes. Excessively acidic or alkaline environments can cause significant damage to tissues. The alternating pulsed electric field constructed using a triboelectric nanogenerator continuously changes the redox reaction near the electrodes, thus maintaining the pH near the electrodes. This overcomes the damage to surrounding tissues caused by traditional electrostimulation therapy and offers better tissue biocompatibility.

[0007] Thirdly, the construction of the electric field by the triboelectric nanogenerator not only achieves the polarization and accumulation of surface charge, but also increases the electron mobility inside the material and reduces the efficiency of electron annihilation. Therefore, it greatly improves the electron utilization rate and catalytic efficiency in the catalytic process. At the same time, under the action of the electric field, the lipid bilayer of the cell membrane undergoes instantaneous disorder, the membrane permeability increases, and "perforations" are formed, which is conducive to the entry of reactive oxygen molecules and improves the effect of catalytic therapy.

[0008] Fourthly, conductive hydrogels, as a matrix rich in nanocatalysts, not only have good biocompatibility, but also provide a good platform for the aggregation and electron migration of nanocatalysts.

[0009] In a first aspect, embodiments of this application provide a self-powered system for catalytic therapy, the system comprising:

[0010] Triboelectric nanogenerators are used to generate alternating current pulses.

[0011] The first electrode is connected to one end of the triboelectric nanogenerator;

[0012] The second electrode is connected to the other end of the triboelectric nanogenerator;

[0013] In this configuration, a pulsed electric field exists between the first electrode and the second electrode, and a conductive hydrogel containing nanocatalysts and a physiological buffer containing cells are placed between the first electrode and the second electrode. The cells and the nanocatalysts undergo catalytic therapy under the action of the alternating pulsed electric field, so that the reactive oxygen molecules generated by the nanocatalysts enter the cells.

[0014] In some possible embodiments, the triboelectric nanogenerator is an interdigitated triboelectric nanogenerator.

[0015] In some possible embodiments, the interdigitated triboelectric nanogenerator includes:

[0016] The fixed component includes a first triboelectric conductive unit and a second triboelectric conductive unit, wherein the first triboelectric conductive unit includes a third electrode composed of conductive monomers, and the second triboelectric conductive unit includes a fourth electrode composed of conductive monomers;

[0017] The sliding component corresponding to the fixed component includes a friction unit;

[0018] The fixed component and the sliding component undergo sliding friction to generate a potential difference between the third electrode and the fourth electrode, thereby obtaining the alternating pulse current.

[0019] In some possible embodiments, the fixing component further includes:

[0020] A support layer is attached to the outside of the triboelectric conductive unit to provide support for the triboelectric conductive unit.

[0021] In some possible embodiments, the first electrode and / or the second electrode are silver needle electrodes; the preset length range of the silver needle electrodes is 0.2 to 0.8 cm, and the preset preferred length is 0.2 cm.

[0022] In some possible embodiments, the nanocatalyst is any one or a combination of nano-noble metal catalysts, single-atom nanocatalysts, nano-carbon catalysts, and nano-transition metal catalysts.

[0023] In some possible embodiments, the conductive hydrogel is an electronically conductive hydrogel, and the material of the electronically conductive hydrogel includes at least one of the following materials:

[0024] Metal nanomaterials, carbon-based materials, and conductive polymer materials.

[0025] In some possible embodiments, the preset distance between the first electrode and the second electrode is 0.1 to 2 cm, and the preset preferred distance is 0.5 cm.

[0026] In some possible embodiments, the preset cell concentration range of the physiological buffer solution is 100,000 to 2,000,000 cells / mL.

[0027] Secondly, embodiments of this application also provide a catalytic treatment method, applied to any of the above-mentioned self-powered systems for catalytic treatment, wherein the cells are subjected to catalytic treatment under the action of the pulsed electric field, so that the reactive oxygen molecules generated by the nanocatalyst enter the cells.

[0028] Other features and advantages of this application will be set forth in the description which follows, and will be apparent in part from the description, or may be learned by practicing the application. The objectives and other advantages of this application may be realized and obtained by means of the structures particularly pointed out in the written description, claims, and drawings. Attached Figure Description

[0029] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments of this application will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0030] Figure 1 This is a structural diagram of a self-powered system for catalytic therapy, as shown in an embodiment of this application.

[0031] Figure 2 This is a schematic diagram of an interdigitated triboelectric nanogenerator shown in an embodiment of this application;

[0032] Figure 3a This is a schematic diagram of the open-circuit voltage of an AC pulse circuit as shown in an embodiment of this application;

[0033] Figure 3b This is a schematic diagram of the short-circuit current of an AC pulse circuit as shown in an embodiment of this application;

[0034] Figure 4a The image shown is a scanning electron microscope image of the covalent organic framework nanocatalyst illustrated in the embodiments of this application.

[0035] Figure 4b Transmission electron microscope (TEM) images and high-resolution images of the nanocatalysts shown in the embodiments of this application;

[0036] Figure 4c Here is a scanning electron microscope image of the gel shown in the embodiments of this application;

[0037] Figure 4d The image shown is a scanning electron microscope image of a permeable nanocatalyst as illustrated in the embodiments of this application.

[0038] Figure 5a This is a statistical diagram of the state of 4T1 cells under different stimuli, as shown in the embodiments of this application;

[0039] Figure 5b This is a statistical chart illustrating the killing ability of 4T1 cells under different stimuli in the embodiments of this application;

[0040] Figure 5c This is a statistical graph of fluorescence intensity generated under different stimuli, as shown in the embodiments of this application;

[0041] Figure 5d This is a statistical graph illustrating the changes in cell membrane potential fluorescence intensity in an embodiment of this application;

[0042] Figure 6 This is a statistical chart showing the results of tumor treatment in mice as illustrated in the embodiments of this application;

[0043] Figure 7 This is a flowchart illustrating the catalytic treatment method according to an embodiment of this application. Detailed Implementation

[0044] The technical solutions in the embodiments of this application will be clearly and thoroughly described below with reference to the accompanying drawings. In the description of the embodiments of this application, unless otherwise stated, "and / or" means "or", for example, A / B can mean A or B; "and / or" in the text is merely a description of the relationship between related objects, indicating that there can be three relationships, for example, A and / or B can mean: A exists alone, A and B exist simultaneously, and B exists alone. In addition, in the description of the embodiments of this application, "multiple" means two or more.

[0045] In the description of the embodiments of this application, unless otherwise stated, the term "multiple" refers to two or more, and other quantifiers are similarly understood. The preferred embodiments described herein are for illustration and explanation only and are not intended to limit this application. Furthermore, the embodiments and features in the embodiments of this application can be combined with each other without conflict.

[0046] To further illustrate the technical solutions provided in the embodiments of this application, a detailed description is provided below in conjunction with the accompanying drawings and specific implementation methods. Although the embodiments of this application provide method operation steps as shown in the following embodiments or drawings, more or fewer operation steps may be included in the method based on conventional or non-inventive effort. For steps that do not logically have a necessary causal relationship, the execution order of these steps is not limited to the execution order provided in the embodiments of this application. In actual processing or when the control device executes the method, it may be executed sequentially or in parallel according to the method shown in the embodiments or drawings.

[0047] Among related technologies, nanocatalysts exhibit relatively low catalytic efficiency. While applying an external stimulus such as an electric field can improve the catalytic activity of nanocatalysts to some extent, the construction of the electric field is limited by external devices. This not only increases the complexity of the treatment but also imposes significant spatiotemporal constraints on patients, ultimately reducing treatment efficiency.

[0048] To address the aforementioned issues, this application's embodiments utilize the characteristics of triboelectric nanogenerators, which can convert mechanical energy into electrical energy and provide pulse output, to replace traditional power supply equipment in providing alternating electric fields. This results in a miniaturized, highly biocompatible, and effective treatment system that is energy-efficient and economical. Figure 1 This is a structural diagram of a self-powered system for catalytic therapy provided in an embodiment of this application. Figure 1As shown, the system includes a triboelectric nanogenerator, a first electrode, a second electrode, and a buffer solution and a conductive hydrogel containing a nanocatalyst placed between the two electrodes. The buffer solution serves as both a dielectric and maintains the normal physiological state of the cells. The triboelectric nanogenerator generates alternating pulsed current; the first electrode is connected to one end of the triboelectric nanogenerator; the second electrode is connected to the other end of the triboelectric nanogenerator; an alternating pulsed electric field exists between the first and second electrodes, and the conductive hydrogel containing the nanocatalyst and the physiological buffer solution (e.g., phosphate buffer) containing the cells are also placed there. Under the action of the alternating pulsed electric field, the cells undergo field-enhanced catalytic therapy. Under the action of the electric field, the nanocatalyst generates a large number of reactive oxygen molecules, and the cell membrane permeability increases, leading to cell death.

[0049] In some possible embodiments, the first and second electrodes described above are metallic electrodes. To ensure the biocompatibility of both electrodes with cells, the electrodes should be made of stable and non-toxic materials. For example, metallic silver is chosen as the first and / or second electrode to enhance the electric field range and better facilitate in vivo experiments. Specifically, metal needles (gold, silver, aluminum) can be selected as electrodes to reduce tissue damage during surgical implantation. Furthermore, a certain distance needs to be maintained between the first and second electrodes for placing buffer solution at this distance during in vitro experiments and for constructing the electric field during in vivo experiments. Based on extensive experimental testing, the optimal distance range in this application is 0.5-1 cm.

[0050] Considering that the construction of interdigitated electrodes can easily achieve the creation of high-pulse-frequency electric fields, the triboelectric nanogenerator in this embodiment can be an interdigitated triboelectric nanogenerator. To facilitate understanding of the technical solution provided in this embodiment, an interdigitated triboelectric nanogenerator will be used as an example below.

[0051] Interdigitated triboelectric nanogenerators consist of fixed and sliding components, specifically as follows: Figure 2 As shown, the fixed component includes a first triboelectric conductive unit and a second triboelectric conductive unit. The sliding component includes a friction unit. Simple sliding friction can occur between the sliding component and the fixed component. Each of the two triboelectric conductive units includes multiple conductive monomers (in this embodiment, the number is 10, i.e., each triboelectric conductive unit includes 5 conductive monomers).

[0052] To ensure the stability of voltage output and electric field construction, the conductive cells in the first and second triboelectric conductive units are preferably arranged in a staggered pattern at equal intervals. The first ends of the conductive cells in the first triboelectric conductive unit are connected to form a third electrode, and the tail ends of the conductive cells in the second triboelectric conductive unit are connected to form a fourth electrode. During the sliding friction process, the sliding component slides relative to the fixed component, generating a potential difference on the third and fourth electrodes. The triboelectric conductive unit also includes a friction layer, which is attached to the surfaces of the third and fourth electrodes and undergoes sliding friction with the friction layer during the sliding process of the sliding component.

[0053] In some possible embodiments, the fixing component may further include a support layer that fits against the outside of the triboelectric unit to provide support for the triboelectric unit.

[0054] In some possible embodiments, the sliding component can be attached to a variety of sliding components or to a living organism, such as a movable part like a finger.

[0055] When the triboelectric conductive units of the interdigitated triboelectric nanogenerator undergo sliding friction with each other, alternating current pulses are generated on the triboelectric conductive units. Specifically, this can be achieved as follows: Figure 3a and Figure 3b As shown. Among them, Figure 3a and Figure 3b This is a schematic diagram of the open-circuit voltage and short-circuit current of the AC pulse current in the embodiments of this application.

[0056] Conventional power supplies often struggle to generate high-frequency AC pulses, while triboelectric nanogenerators provide AC pulses with frequencies ranging from 0-40Hz and voltages from 0-50V. One end of the triboelectric nanogenerator is connected to a first electrode, and the other end to a second electrode, generating a pulsed electric field on the nanocatalysts and cells dispersed between the two electrodes. This enhances the catalytic activity of the nanocatalysts and promotes cell-killing effects.

[0057] In some possible embodiments, a layer of copper with a thickness of 0.5 mm can be uniformly coated on the surface of the acrylic sheet, and then a portion of the acrylic sheet is removed at equal intervals according to the pattern requirements. This forms the desired triboelectric conductive unit.

[0058] Furthermore, to further enhance the activity of nanocatalysts under alternating electric fields, covalent organic framework nanocatalysts can be used as electronically tunable nanocatalyst platforms. Simultaneously, electronically conductive hydrogels poly(3,4-ethylenedioxythiophene) and polystyrene sulfonate hydrogels (PEDOT:PSS) were selected as the matrix material for the doped covalent organic framework nanocatalysts. This conductive hydrogel (PC) with nanocatalyst activity can provide a platform for electron transfer, which is beneficial for studying the influence of alternating electric fields on the activity of nanocatalysts.

[0059] Cells are suspended in a buffer solution containing nanocatalysts. The cell concentration in the buffer solution should be controlled within the range of 10-2 million / ml. This is because this concentration range can ensure sufficient cell density as much as possible without affecting the normal physiological state of the cells.

[0060] In some possible embodiments, the cell can be a mammalian cell or other, and the nanocatalyst can be at least one of noble metal nanocatalysts (gold, platinum, palladium), single-atom nanocatalysts, carbon nanocatalysts (graphene, carbon nanotubes) and transition metal nanocatalysts (metal-organic frameworks, covalent organic frameworks).

[0061] In in vivo experiments, mice can be selected as animal models. A xenograft is used to transplant a tumor into the mouse abdomen. Subsequently, a triboelectric nanogenerator is used to provide an alternating electric field, enhancing the catalytic activity of the nanocatalyst at the tumor site, thereby achieving the goal of tumor therapy. Specifically, as follows... Figures 4a to 4d As shown, where, Figure 4a This is a scanning electron microscope (SEM) image of a covalent organic framework nanocatalyst. Figure 4b Transmission electron microscopy (TEM) images and high-resolution images of covalent organic framework nanocatalysts. Figure 4c This is a scanning electron microscope (SEM) image of poly(3,4-ethylenedioxythiophene) and polystyrene sulfonate hydrogel (PEDOT:PSS). Figure 4d Scanning electron microscope (SEM) image of a covalent organic framework nanocatalyst (PC) incorporated into a polystyrene sulfonate hydrogel.

[0062] It should be understood that, due to the existence of different crystal plane indices, high-resolution imaging can reveal the successful coating of carbon nanotubes with a covalent organic framework. Therefore, according to... Figure 4b The transmission electron microscopy (TEM) images and high-resolution images show the successful preparation of covalent organic framework nanocatalysts. Furthermore, through... Figure 4c and Figure 4d It can be seen that the number of pores decreased and the pore size increased to a certain extent, which is due to the partial collapse of the pores. However, the overall PEDOT:PSS hydrogel still exhibits a porous state. Therefore, it can be seen that the incorporation of covalent organic framework nanocatalysts has no significant impact on the overall structure of the hydrogel.

[0063] The effects of a self-powered system for catalytic therapy provided in this application will be described below with reference to Examples 1 and 2.

[0064] Example 1

[0065] In this embodiment, conductive hydrogel (PC) with nanocatalyst activity was soaked in PBS for 2 days, followed by UV sterilization for 2 hours. 4T1 cells were then digested and dispersed in culture medium at a cell density of 2 million / ml. The cells were fixed in cell culture plates, sterilized PC was added, and silver electrodes were fixed in the cell culture plates. The two ends of the silver electrodes were connected to the two ends of a triboelectric nanogenerator. The surface of the triboelectric nanogenerator was then rubbed with a finger at a frequency of 3.5 Hz for 3 minutes. Different conditions were applied to the control group. After the application, the culture medium was aspirated, and the cells were washed twice with PBS. The cells were then cultured with 0.1 mg / ml calcein and propidium iodide (PI) for 30 minutes, washed twice with PBS, and observed using a confocal microscope. The results are as follows. Figure 5a and 5b As shown. Among them, Figure 5a From top to bottom, each row represents the results of the killing ability of 4T1 cells under different stimuli in Example 1, the results of the changes in the level of reactive oxygen molecules in the cells under the stimulation of triboelectric nanogenerators, the results of cell apoptosis under the stimulation of triboelectric nanogenerators, and the results of the changes in cell membrane potential under the stimulation of triboelectric nanogenerators. Figure 5b Statistical analysis of the killing ability of 4T1 cells under different stimuli in Example 1.

[0066] The cell viability of the nanocatalyst under triboelectric nanogenerator stimulation was only 24.8%. Subsequently, using a similar method, the ability of the nanocatalyst to generate reactive oxygen species under triboelectric nanogenerator stimulation was evaluated using 2',7'-dichlorodihydrofluorescein diacetate. The results are as follows: Figure 5a and 5c As shown, where, Figure 5c The statistical results of fluorescence intensity characterization of reactive oxygen species produced under different stimuli in Example 1 are presented. Figure 5c It was found that the production level of reactive oxygen species after stimulation increased by 24.6 times compared with the control group. Apoptosis was assessed using a mitochondrial membrane potential assay kit, and the results were as follows... Figure 5a and 5d As shown, cells containing nanocatalysts underwent significant apoptosis under the stimulation of a triboelectric nanogenerator. Finally, changes in cell membrane potential were detected, such as... Figure 5a and 5d As shown, where, Figure 5d The changes in cell membrane potential fluorescence intensity at different durations of electrical stimulation in Example 1 were characterized. Figure 5d It can be seen that the fluorescence intensity increased by 2.12 times under the stimulation of the triboelectric nanogenerator, indicating an increase in cell membrane potential and enhanced cell membrane permeability.

[0067] Example 2

[0068] In this embodiment, after xenografting of an abdominal tumor in BALB / c mice, a conductive hydrogel (PC) with nanocatalyst activity was locally injected into the tumor site. Two silver needles were inserted into the tumor site as the first and second electrodes, respectively. Subsequently, an alternating electric field was constructed at the tumor site using a triboelectric nanogenerator. Under the combined action of the triboelectric nanogenerator and the nanocatalyst, the tumor inhibition rate reached 84.2%. The treatment results and optical photographs are shown below. Figure 6 As shown.

[0069] As can be seen from the above embodiments 1 and 2, this application utilizes the pulse output and self-driven electric field construction characteristics of a triboelectric nanogenerator to replace traditional power supply devices. This makes the entire device structure provided by this application embodiment (see...)... Figure 1 It possesses advantages such as miniaturization, energy efficiency, and wearability. Furthermore, the introduction of silver needle electrodes can reduce tissue damage. Therefore, the self-powered system and method for catalytic therapy provided in this application embodiment will have broad application prospects in the field of catalytic therapy.

[0070] Based on the same inventive concept, this application also provides a catalytic therapy method applied to the aforementioned self-powered system for catalytic therapy. Under the action of the pulsed electric field, the cells are subjected to catalytic therapy, allowing reactive oxygen molecules generated by the nanocatalyst to enter the cells and kill them. The general processing flow of this method is as follows: Figure 7 As shown, it includes the following steps:

[0071] Step 701: Connect the first electrode and the second electrode to the well plate, sterilize them, and then connect them to the triboelectric nanogenerator.

[0072] Step 702: Culture the cells in a culture medium containing nanocatalysts.

[0073] Step 703: The triboelectric nanogenerator generates alternating current pulses by rubbing the triboelectric layers together, which electrically stimulate the nanocatalysts and cells dispersed between the two electrodes, thereby generating reactive oxygen molecules and allowing them to enter the cells.

[0074] Step 704: Inject the nanocatalyst in situ into the tumor site of the mouse.

[0075] Step 705: The triboelectric nanogenerator generates alternating current pulses by friction between the triboelectric layers, which electrically stimulate the nanocatalysts dispersed at the tumor site, thereby treating the tumor.

[0076] In summary, this application demonstrates that the self-driven electric field stimulation provided by a triboelectric nanogenerator can significantly enhance the catalytic activity of nanocatalysts. Utilizing the self-driven electric field construction, miniaturization, and pulsed output characteristics of the triboelectric nanogenerator, it replaces the traditional external power source, overcoming the spatiotemporal limitations during treatment and achieving catalytic therapy enhanced by a self-powered electric field. Furthermore, the pulses from the triboelectric nanogenerator not only reduce tissue damage but also increase cell membrane permeability, enhancing the therapeutic effect.

[0077] Those skilled in the art will understand that embodiments of this application can be provided as methods, systems, or computer program products. Therefore, this application can take the form of a completely hardware embodiment, a completely software embodiment, or an embodiment combining software and hardware aspects. Furthermore, this application can take the form of a computer program product embodied on one or more computer-usable storage media (including but not limited to disk storage, CD-ROM, optical storage, etc.) containing computer-usable program code.

[0078] This application is described with reference to flowchart illustrations and / or block diagrams of methods, apparatus (systems), and computer program products according to this application. It should be understood that each block of the flowchart illustrations and / or block diagrams, and combinations of blocks in the flowchart illustrations and / or block diagrams, can be implemented by computer program instructions. These computer program instructions can be provided to a processor of a general-purpose computer, special-purpose computer, embedded processor, or other programmable data processing apparatus to produce a machine, such that the instructions, which execute via the processor of the computer or other programmable data processing apparatus, generate instructions for implementing the flowchart illustrations. Figure 1 One or more processes and / or boxes Figure 1 A device that provides the functions specified in one or more boxes.

[0079] These computer program instructions may also be stored in a computer-readable storage medium that can direct a computer or other programmable data processing device to function in a particular manner, such that the instructions stored in the computer-readable storage medium produce an article of manufacture including instruction means, which are implemented in a process Figure 1 One or more processes and / or boxes Figure 1 The function specified in one or more boxes.

[0080] These computer program instructions may also be loaded onto a computer or other programmable data processing equipment to cause a series of operational steps to be performed on the computer or other programmable equipment to produce a computer-implemented process, thereby providing instructions that execute on the computer or other programmable equipment for implementing the process. Figure 1 One or more processes and / or boxes Figure 1 The steps of the function specified in one or more boxes.

[0081] Obviously, those skilled in the art can make various modifications and variations to this application without departing from the spirit and scope of this application. Therefore, if such modifications and variations fall within the scope of the claims of this application and their equivalents, this application also intends to include such modifications and variations.

Claims

1. A self-powered system for catalytic therapy, characterized in that, The system includes: Triboelectric nanogenerators are used to generate alternating current pulses. The first electrode is connected to one end of the triboelectric nanogenerator; The second electrode is connected to the other end of the triboelectric nanogenerator; In this configuration, a pulsed electric field exists between the first electrode and the second electrode, and a conductive hydrogel containing nanocatalysts and a physiological buffer solution containing cells are placed between the first electrode and the second electrode. The cells and the nanocatalysts undergo catalytic therapy under the action of the alternating pulsed electric field, so that the reactive oxygen molecules generated by the nanocatalysts enter the cells.

2. The system according to claim 1, characterized in that, The triboelectric nanogenerator is an interdigitated triboelectric nanogenerator, which generates AC pulses with a frequency of 0-40Hz and a voltage of 0-50V.

3. The system according to claim 2, characterized in that, The interdigitated triboelectric nanogenerator includes: The fixed component includes a first triboelectric conductive unit and a second triboelectric conductive unit, wherein the first triboelectric conductive unit includes a third electrode composed of conductive monomers, and the second triboelectric conductive unit includes a fourth electrode composed of conductive monomers; The sliding component corresponding to the fixed component includes a friction unit; The fixed component and the sliding component undergo sliding friction to generate a potential difference between the third electrode and the fourth electrode, thereby obtaining the alternating pulse current.

4. The system according to claim 3, characterized in that, The fixing component also includes: A support layer is attached to the outside of the triboelectric conductive unit to provide support for the triboelectric conductive unit.

5. The system according to any one of claims 1-4, characterized in that, The first electrode and / or the second electrode are silver needle electrodes; the length of the silver needle electrode is in the range of 0.2 to 0.8 cm.

6. The system according to claim 1, characterized in that, The nanocatalyst is any one of nano-noble metal catalysts, single-atom nanocatalysts, nano-carbon catalysts, and nano-transition metal catalysts, or a combination thereof.

7. The system according to claim 1, characterized in that, The conductive hydrogel is an electronically conductive hydrogel, and the material of the electronically conductive hydrogel includes at least one of the following materials: Metal nanomaterials, carbon-based materials, and conductive polymer materials.

8. The system according to claim 5, characterized in that, The distance between the first electrode and the second electrode is 0.1 to 2 centimeters.

9. The system according to any one of claims 1-4 and 6-8, characterized in that, The cell concentration range of the physiological buffer solution is 100,000 to 2,000,000 cells / mL.

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