A feed-grade natural colorant and its preparation method and application

Abstract

The present invention provides a feed-grade natural colorant and its preparation method and application. The preparation method of the feed-grade natural colorant includes the step of mixing a lipase oil suspension with a natural pigment extract for enzymolysis; the lipase oil suspension is composed of lipase, water, Tween 60, monoglyceride and grease, wherein the mass content of lipase is 3-10%, and the mass contents of Tween 60, monoglyceride and grease are 30-40%, 20-30% and 10-20% respectively. After the natural pigment extract is enzymolyzed with the lipase oil suspension, the absorption of the natural pigment in the small intestine can be greatly increased, and its bioavailability can be improved, thereby being applied to feed to improve egg yolk yolk color, fish skin color, etc., and the raw materials of the product of the present invention are all natural substances, with advantages such as safety and no toxic side effects, and can completely replace the use of chemically synthesized pigments in animal feed.

Description

Technical Field

[0001] The present invention relates to the field of feed additives, in particular to a feed-grade natural colorant and a preparation method and application thereof. Background Art

[0002] With the development of society and the increasing consumption level, people are becoming more and more particular about the color of egg yolks. Chemical synthetic pigments are bright in color and cheap, but they have various safety risks after use. Therefore, natural plant-based bioactive ingredients are more preferred in feed additives.

[0003] Capsanthin is a natural carotenoid pigment with anti-inflammatory, antioxidant, and lipid peroxide-regulating properties. Its safety has been recognized, making it a natural additive with unlimited use. It is also used as a natural colorant in feed, particularly for egg yolk coloring.

[0004] Lutein, also known as phytolutein, is a carotenoid found in a wide range of plants, including vegetables, fruits, and flowers. Marigolds contain the highest concentration of lutein. Lutein is a fat-soluble vitamin whose absorption spectrum includes near-blue-violet light, helping the retina protect against ultraviolet rays. Therefore, it is widely used in health supplements and foods. Lutein is also a valuable natural colorant, used as a feed additive to enhance the color of meat and improve egg quality.

[0005] However, the lutein and capsanthin products currently used in the feed field, on the one hand, have a low proportion of effective ingredients due to defects in the preparation process; on the other hand, since natural carotenoids themselves have the defect of low bioavailability in animals, large amounts of them need to be added to achieve the desired results, which greatly increases the cost and greatly limits the use prospects of natural pigments in the feed field. Summary of the Invention

[0006] In response to the problems existing in the prior art, the present invention provides a feed-grade natural colorant and a preparation method and application thereof, which can significantly increase the ratio and bioavailability of lutein and capsanthin monomers.

[0007] The present invention provides a method for preparing a feed-grade natural colorant, comprising the steps of mixing a lipase oil suspension with a natural pigment extract and performing enzymolysis;

[0008] The lipase oil suspension consists of lipase, water, Tween 60, monoglyceride and oil, wherein the mass content of lipase is 3-10%, and the mass contents of Tween 60, monoglyceride and oil are 30-40%, 20-30% and 10-20% respectively.

[0009] The invention can greatly increase the absorption of natural pigments in the small intestine and improve their bioavailability after enzymatic hydrolysis of natural pigment extracts with lipase oil suspension, so that they can be applied to feed to improve the yolk color of egg and the skin color of fish. Moreover, the raw materials of the product of the invention are all natural substances, which has the advantages of being safe and having no toxic side effects, and can completely replace the use of chemically synthesized pigments in animal feed.

[0010] Among them, the emulsifier system of the lipase oil suspension is very critical. The inventors tried many systems and unexpectedly found that only the emulsifier system of the present invention can achieve better results.

[0011] According to the preparation method of the feed-grade natural colorant provided by the present invention, the mass ratio of the lipase oil suspension to the natural pigment extract is 2-5:100.

[0012] According to the preparation method of the feed-grade natural colorant provided by the present invention, the enzymatic hydrolysis condition is to stir the enzymatic hydrolysis reaction at a temperature of 37-42° C. for 2-4 hours.

[0013] According to the method for preparing a feed-grade natural colorant provided by the present invention, the natural pigment extract is lutein extract and / or capsanthin extract. The extract can be homemade or obtained through regular commercial channels.

[0014] According to the method for preparing a feed-grade natural colorant provided by the present invention, the natural pigment extract is heated and dissolved at a temperature of 35-42°C before mixing. Preferably, the dissolution time is 10-12 hours. Heating and dissolving the natural pigment extract facilitates uniform mixing and reaction with the lipase oil suspension.

[0015] According to the preparation method of the feed-grade natural colorant provided by the present invention, the oil is an edible oil, for example, soybean oil, sunflower oil, peanut oil, corn oil and other common edible oils.

[0016] According to the preparation method of the feed-grade natural colorant provided by the present invention, the lipase mainly includes carboxylesterase.

[0017] According to the preparation method of the feed-grade natural colorant provided by the present invention, a mixed oil body of pigment micelles is obtained after enzymatic hydrolysis, the ineffective components are removed by adjusting the pH, and then an emulsifier, a dispersant and a diluent are added, and the mixture is stirred and mixed at 45-55°C.

[0018] Adjusting the pH to remove ineffective components refers to adjusting the pH to neutral using an acidic or alkaline solution. The acidic solution may be a phosphoric acid solution or a hydrochloric acid solution. The alkaline solution may be a sodium hydroxide solution or a potassium hydroxide solution. The ineffective components are primarily epoxides.

[0019] Furthermore, based on the quality of the final product, the mass proportions of the emulsifier, dispersant and diluent are 2-5%, 10-30% and 45-65% respectively.

[0020] Furthermore, the emulsifier includes propylene glycol and / or glycerol, the dispersant is silicon dioxide, and the diluent includes talc, stone powder, rice husk powder and / or wheat bran.

[0021] Furthermore, the mixed product is crushed by a crusher and then vacuum-packed.

[0022] In a second aspect, the present invention further provides a feed-grade natural colorant prepared by the above preparation method.

[0023] When the natural pigment extract is lutein extract, the content of trans-lutein in the obtained feed-grade natural colorant is more than 95%.

[0024] When the natural pigment extract is capsicum red extract, the content of trans capsicum red in the obtained feed-grade natural colorant is more than 40%.

[0025] In a third aspect, the present invention further provides a feed comprising the above-mentioned feed-grade natural colorant.

[0026] The feed of the present invention can be poultry feed or aquatic feed, and the colorant is added according to the amount of colorant added in the feed.

[0027] The present invention provides a feed-grade natural colorant, a preparation method thereof, and an application thereof. By enzymatically hydrolyzing a natural pigment extract with a lipase oil suspension, the absorption of the natural pigment in the small intestine can be greatly increased, thereby improving its bioavailability. Thus, the natural pigment can be applied to feed to improve the yolk color of egg whites and the color of fish skin. Moreover, the raw materials of the product of the present invention are all natural substances, and the product has the advantages of being safe and having no toxic side effects. The product can completely replace the use of chemically synthesized pigments in animal feed. DETAILED DESCRIPTION

[0028] To make the objectives, technical solutions, and advantages of the present invention more clear, the technical solutions of the present invention are described clearly and completely below. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0029] Unless otherwise specified, the raw materials involved in the following examples can be obtained from regular commercial channels.

[0030] Among them, lipase was purchased from Xiasheng Biotechnology Development Co., Ltd.

[0031] Lutein extract and capsanthin extract are both from Chenguang Biotechnology Group Co., Ltd.

[0032] Example 1

[0033] This embodiment provides a feed-grade lutein powder, the preparation method of which is as follows:

[0034] Take 100g of lutein extract and dissolve it at 40℃ for 10h; prepare a 3% lipase oil suspension according to the mass proportions of lipase, water, Tween60, monoglyceride and soybean oil, respectively, 3%, 17%, 40%, 30% and 10%, add 5g of lipase oil suspension to the lutein extract and stir at 40℃ for 2h, slowly add 1% phosphoric acid solution to adjust the pH value of the oil to neutral, and the pH adjustment process can remove invalid components; add 25g of propylene glycol, 200g of silicon dioxide and 550g of stone powder and stir and mix them evenly at 50℃, and then crush to obtain 880g of feed-grade lutein powder.

[0035] Comparative Example 1

[0036] This comparative example provides a feed-grade lutein powder, the preparation method of which is as follows:

[0037] Take 100g of lutein extract and dissolve it at 40℃ for 10h; prepare a 3% lipase oil suspension according to the mass proportions of lipase, water, Tween80, monoglyceride and soybean oil, respectively, 3%, 17%, 40%, 30% and 10%, add 5g of lipase oil suspension to the lutein extract and stir at 40℃ for 2h, slowly add 1% phosphoric acid solution to adjust the pH value of the oil to neutral; add 25g of propylene glycol, 200g of silicon dioxide and 550g of stone powder and stir and mix uniformly at 50℃, and then crush to obtain 880g of feed-grade lutein powder.

[0038] Comparative Example 2

[0039] This comparative example provides a feed-grade lutein powder, the preparation method of which is as follows:

[0040] Take 100g of lutein extract and dissolve it at 40℃ for 10h; prepare a 3% lipase oil suspension according to the mass proportions of lipase, water, Tween60, monoglyceride and soybean oil, respectively, 3%, 12%, 50%, 30% and 5%, add 5g of lipase oil suspension to the lutein extract and stir at 40℃ for 2h, slowly add 1% phosphoric acid solution to adjust the pH value of the oil to neutral; add 25g of propylene glycol, 200g of silicon dioxide and 550g of stone powder and stir and mix them evenly at 50℃, and then crush to obtain 880g of feed-grade lutein powder.

[0041] Comparative Example 3

[0042] This comparative example provides a lutein crystal, and the preparation method thereof is as follows:

[0043] 100 g of lutein extract was mixed with 225 ml of ethanol, heated to 65°C, and stirred to form a homogeneous solution; 1 g of lipase was dissolved in 95 ml of water to form an enzyme solution, which was added to the homogeneous solution. Enzymatic hydrolysis was carried out at 65°C for 18 hours. The reaction solution was cooled to 65°C and filtered while hot to obtain a yellow filter cake. The filter cake was dissolved in ethyl acetate, washed with deionized water, the ethyl acetate was recovered, frozen for crystallization, centrifuged, and vacuum dried to obtain lutein crystals.

[0044] Example 2

[0045] This embodiment provides a feed-grade capsanthin powder, the preparation method of which is as follows:

[0046] Take 150g of capsanthin extract and dissolve it at 35℃ for 12h; prepare a 5% lipase oil suspension according to the proportions of lipase, water, Tween60, monoglyceride and soybean oil, respectively, 5g of lipase oil suspension is added to the capsanthin extract and stirred at 40℃ for 2h, and 1% phosphoric acid solution is slowly added dropwise to adjust the pH value of the oil to neutral; add 50g of propylene glycol, 350g of silicon dioxide and 700g of stone powder and stir and mix them evenly at 50℃, and then crush to obtain 1260g of feed-grade capsanthin powder.

[0047] Comparative Example 4

[0048] This comparative example provides a feed-grade capsanthin powder, the preparation method of which is as follows:

[0049] Take 150g of capsanthin extract and dissolve it at 35℃ for 12h; prepare a 5% lipase oil suspension according to the proportions of lipase, water, Tween80, monoglyceride and soybean oil, respectively, 5g of lipase oil suspension is added to the capsanthin extract and stirred at 40℃ for 2h, and 1% phosphoric acid solution is slowly added dropwise to adjust the pH value of the oil to neutral; add 50g of propylene glycol, 350g of silicon dioxide and 700g of stone powder and stir and mix them evenly at 50℃, and then crush to obtain 1260g of feed-grade capsanthin powder.

[0050] Comparative Example 5

[0051] This comparative example provides a feed-grade capsanthin powder, the preparation method of which is as follows:

[0052] Take 150g of capsanthin extract and dissolve it at 35℃ for 12h; prepare a 5% lipase oil suspension according to the proportions of lipase, water, Tween60, monoglyceride and soybean oil of 5%, 10%, 50%, 30% and 5% respectively, then add 5g of lipase oil suspension to the capsanthin extract and stir at 40℃ for 2h, slowly add 1% phosphoric acid solution to adjust the pH value of the oil to neutral; add 50g of propylene glycol, 350g of silicon dioxide and 700g of stone powder and stir and mix them evenly at 50℃, and then crush to obtain 1260g of feed-grade capsanthin powder.

[0053] Experimental example

[0054] The additive samples prepared in the above-mentioned examples and comparative examples were mixed with the same amount of conventional alkali saponification product (see the literature "Study on the Saponification Process of Lutein Extract" by Li Gaofeng et al., 2009) and chicken feed to form a diet with an additive content of 20 ppm. Coloring experiments were conducted in laying hens:

[0055] Each experimental group consisted of 30 laying hens, fed the above diet for 30 consecutive days. Ten eggs were randomly selected from each group on day 30 and the group average was determined. The results are shown in the table below.

[0056] Table 1

[0057] 20ppm diet corresponding sample name Egg yolk color value (average) Trans ratio Lye saponified lutein 8.5 85％ Example 1 Sample 9.3 96％ Comparative Example 1 Sample 8.9 90％ Comparative Example 2 Sample 8.7 88％ Comparative Example 3 Sample 9.0 92％ Alkali saponified capsanthin 11.0 37％ Example 2 Sample 11.7 43％ Comparative Example 4 Sample 11.2 38％ Comparative Example 5 Sample 10.7 35％

[0058] The experimental results show that enzymatic hydrolysis with a specific lipase oil suspension can reduce the loss of lutein during processing, increase the proportion of trans-lutein and trans-capsanthin in the product, and significantly improve the coloring effect, which has a high use value.

[0059] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A method for preparing a feed-grade natural colorant, characterized in that: The method comprises the steps of mixing a lipase oil suspension with a natural pigment extract for enzymatic hydrolysis; The lipase oil suspension is composed of lipase, water, Tween 60, monoglyceride and oil, wherein the mass content of lipase is 3-10%, and the mass contents of Tween 60, monoglyceride and oil are 30-40%, 20-30% and 10-20% respectively; The natural pigment extract is lutein extract and / or capsanthin extract.

2. The method for preparing a feed-grade natural colorant according to claim 1, wherein The mass ratio of the lipase oil suspension to the natural pigment extract is 2-5:

100.

3. The method for preparing a feed-grade natural colorant according to claim 1, wherein The enzymatic hydrolysis conditions are as follows: stirring the enzymatic hydrolysis reaction at a temperature of 37-42° C. for 2-4 hours.

4. The method for preparing a feed-grade natural colorant according to claim 1, wherein Before mixing, the natural pigment extract is heated and dissolved at a temperature of 35-42°C.

5. The method for preparing a feed-grade natural colorant according to any one of claims 1 to 4, wherein: The grease is edible oil; And / or, the lipase mainly comprises carboxylesterase.

6. The method for preparing a feed-grade natural colorant according to any one of claims 1 to 4, wherein: After enzymatic hydrolysis, a mixed oil body of pigment micelles is obtained, and the ineffective components are removed by adjusting the pH. Then, an emulsifier, a dispersant and a diluent are added and stirred and mixed at 45-55°C.

7. The method for preparing a feed-grade natural colorant according to claim 6, wherein Based on the quality of the final product, the mass proportions of the emulsifier, dispersant and diluent are 2-5%, 10-30% and 45-65% respectively.

8. A feed-grade natural colorant, characterized in that: Prepared by the preparation method according to any one of claims 1 to 7, when the natural pigment extract is lutein extract, the trans-lutein content in the obtained feed-grade natural colorant is more than 95%; when the natural pigment extract is capsanthin extract, the trans-capsanthin content in the obtained feed-grade natural colorant is more than 40%.

9. A feed, characterized in that Including the feed grade natural colorant according to claim 8.