Mouse body fixation clamp

Abstract

A mouse body fixing clamp belongs to a mouse body fixing device. In the process of collecting images of the central nervous system of a mouse, the image is collected by the method of anesthetizing the mouse, but the image obtained by the method influences the research result. The nuclear magnetic bed plate (1) is located at the bottom of the whole mechanism, the preservation tube (4) is inlaidly installed in the preservation warehouse in the nuclear magnetic bed plate (1), the body fixing frame (5) is slidingly installed in the first groove (9), the front end of the body fixing frame (5) is inserted with the air pipe (8), the front upper portion of the body fixing frame (5) is insertedly installed with the neck clamping block (7), the rear upper portion of the body fixing frame (5) is installed with the limiting plate (6), the electromagnetic heating block (2) and the preservation box (3) are arranged from the head to the tail above the nuclear magnetic bed plate (1), and the preservation box (3) is coveringly installed above the body fixing frame (5). The device can better make the mouse body and posture in a static state.

Description

TECHNICAL FIELD

[0001] The present application relates to a mouse body fixation clamp, in particular to a mouse body fixation clamp. BACKGROUND

[0002] Due to the homology of mouse and human genes, the similar proportion is close, and the mouse has fast reproduction and gentle character, and has been used as the preferred animal object for medical research in recent hundreds of years. When the mouse is subjected to in vivo imaging examination, the mouse needs to be absolutely immobilized in an anesthetized state to complete the examination. Resting-state functional magnetic resonance imaging (rsfMRI) is often used for related physiological research, and provides an opportunity for observing the functional neural network, neurotransmitter and neural coupling of the mouse. In order to ensure that the posture of the mouse is in a static state during image acquisition, so as to prevent the generation of motion-related artifacts, so far the vast majority of methods are to conduct rsfMRI research on the mouse in an anesthetized state. Although the function and dynamic structure of the rsfMRI network system can be observed in an anesthetized state, however, anesthetics can change the hemodynamics and neurovascular coupling, or produce unnecessary genetic or pharmacological interactions, which will confuse the mechanism explanation of the rsfMRI signal, as shown in Figure 19 It is found that by comparing the characteristics of the awake network with the characteristics of the network in an anesthetized state, it is confirmed that the awake mouse rsfMRI network topology maximizes the integration between the neural communities and the unique co-activation patterns, including the dominant participation of the wake-related nuclei, the anti-coordination between the visual, auditory and default mode network (DMN) regions, and the special time transition to the unique network attraction state, as shown in Figures 20-21 These results show that the rsfMRI activity of the awake mouse is formed by the state-specific participation of the basal forebrain wake system, and shows a fixed spatiotemporal structure, which restructures the basic principles of the conscious network state of higher mammalian species. Therefore, the study of the rsfMRI of the mouse in an awake state has become a research hotspot at home and abroad. It is necessary to design a device for fixing the posture of the mouse, so as to ensure that the mouse is in an awake state and can maintain a fixed posture during the in vivo imaging examination of the mouse.

[0003] Due to the long imaging time, large noise, high absolute immobilization requirement, and absolute contraindication of metal materials of MRI examination, and the requirements and constraints, the mouse does not have the subjective consciousness control of human beings, and often cannot cooperate with long-time absolute stillness, resulting in large image artifacts, which affects the interpretation of the MRI results. Therefore, different research institutions at home and abroad want to use various means to realize the absolute fixation of the head and neck of the mouse during the micro-MRI examination.

[0004] Existing problems: (1) It is currently difficult to achieve a unified standard for the effect of absolute fixation of mice. At the same time, there is still insufficient adjustment of the size of the fixation device for mice of different sizes. Incompatible models can lead to airway compression and suffocation in mice or spinal fractures, making it impossible to conduct further experimental research; (2) Since the micro-MRI examination of mice takes a long time, improving the comfort of the fixation model is also crucial, such as using the extended position of the mouse limbs, designing molds that conform to the physiological and anatomical position of the mouse, and improving the softness of materials in the buckles, joint fixation positions, etc. Therefore, how to achieve individualized customized mouse fixation models is particularly important.

[0005] Existing mouse restraint devices such as Figures 22-25 9.4T small animal magnetic resonance imaging (MRI) system, such as Figure 25 As shown.

[0006] In summary, currently, there is no specific posture fixation device for acquiring images of the central nervous system in mice. Anesthetizing the mice to ensure a fixed posture affects research results. Alternatively, using boards and ropes to fix the mice provides poor fixation. Therefore, improvements were made to existing fixation clamps, leading to the design of the technical solution in this application. Summary of the Invention

[0007] The purpose of this invention is to address the shortcomings in existing research on mouse body fixation devices by proposing a mouse body fixation clamp.

[0008] A mouse body fixation clamp, the fixation clamp comprising an MRI bed plate, an electromagnetic heating block, an incubator, an incubation tube, a body fixation frame, a limiting plate, a neck clamp, and an air tube;

[0009] The NMR bed plate has a symmetrical structure. The inner side of the NMR bed plate is provided with NMR plate grooves. The length of the NMR plate grooves is adapted to the length of the NMR bed plate. The NMR plate grooves are divided into two sections: a first groove and a second groove.

[0010] The main body of the NMR bed plate is equipped with an insulated chamber, and the end of the insulated chamber is connected to the tail end face of the NMR bed plate.

[0011] The inner surface of the electromagnetic heating block is provided with a third groove, the end of which is connected to the side wall of the electromagnetic heating block; the width of the cross-section of the head end of the electromagnetic heating block is greater than the width of the cross-section of the connecting end, and an electromagnetic coil is provided inside the electromagnetic heating block.

[0012] The insulated box has a box-like structure and is filled with a heat-conducting liquid.

[0013] The insulation pipe has a hollow tube structure;

[0014] The body fixing frame is a hollow cylindrical structure; two ends of the body fixing frame are open structures, and one end of the body fixing frame is tapered;

[0015] The limiting plate is a screw structure with a sheet-shaped part at one end;

[0016] The neck clamping block is a rod structure with a bifurcated part;

[0017] The ventilation pipe is a hollow pipe body;

[0018] The nuclear magnetic bed plate is located at the bottom of the whole mechanism, the heat preservation pipe is embeddedly installed in the heat preservation bin in the nuclear magnetic bed plate, the body fixing frame is slidingly installed in the first groove, the front end of the body fixing frame is inserted with the ventilation pipe, the front upper part of the body fixing frame is insertedly fixed with the neck clamping block, and the rear upper part of the body fixing frame is installed with the limiting plate; the electromagnetic heating block and the heat preservation box are arranged from the head to the tail above the nuclear magnetic bed plate, and the heat preservation box cover is installed above the body fixing frame;

[0019] The body fixing frame further comprises a cabin body opening, a cabin top sliding groove, a clamping sliding plate, a clamping hole, two ear holes, a half shaft pin, two sliding groove blocks, a front hole, a lower hole and a clamping sliding groove.

[0020] The body fixing frame is a hollow left-right symmetrical structure, and the main body part of the body fixing frame is a thin-walled shell structure;

[0021] The cabin body opening is arranged at the rear end of the body fixing frame;

[0022] From the rear to the front of the body fixing frame, the cabin top sliding groove, the clamping sliding plate, the clamping hole and the two ear holes are sequentially arranged along the symmetry line on the top of the body fixing frame; the clamping sliding plate is insertedly arranged on the hole edge of the clamping hole close to the rear of the body fixing frame, and the two ear holes are symmetrically arranged on the two sides of the body fixing frame;

[0023] The front end of the body fixing frame is sequentially provided with the front hole and the lower hole from front to rear along the symmetry line on the bottom of the body fixing frame;

[0024] The front end of the body fixing frame is provided with a half shaft pin below each ear hole;

[0025] The rear part of the body fixing frame is provided with a pair of left-right symmetrical two sliding groove blocks, which are slidingly inserted into the body fixing frame sliding groove on the nuclear magnetic bed plate (1);

[0026] The clamping sliding plate is provided with a long hole clamping sliding groove in the middle of the plate body;

[0027] The cabin top sliding groove, the clamping hole, the two ear holes, the front hole and the lower hole are all through holes; the shape of the cabin top sliding groove is rectangular;

[0028] The cabin top sliding groove, the clamping hole, the two ear holes, the front hole and the lower hole are all through holes; the shape of the cabin top sliding groove is rectangular;

[0029] The size parameters of each part of the body fixing frame are set as follows:

[0030] Let d D represents the diameter of the front hole, which matches the mouth and nose of the mouse. When the mouse's head is inserted into the hole, the mouse's mouth can be opened to allow the mouse's front teeth to bite the bite block, thereby fixing the mouse's head.

[0031] Let θ represent the angle between the upper and lower walls of the front hole. The angle inside the front hole allows the mouse's head to fit snugly and maintain a comfortable state for a long time.

[0032] Let e D represents the diameter of the mouse's head, which is the distance from the front end of the clamping hole to the front end of the lower hole.

[0033] Let f D represents the maximum diameter of the mouse's neck, which is the diameter of the body fixing frame where the clamping slide is installed.

[0034] Let g D represents the diameter of the mouse's torso, which is the diameter of the cabin body.

[0035] Let w D represents the distance from the end of the mouse's head to the front end of the body fixing frame, which is the distance between the front end of the front hole and the middle of the clamping hole in the longitudinal direction of the body fixing frame.

[0036] Let k D represents the distance from the maximum diameter of the mouse's neck to the front end of the body fixing frame, which is the distance between the front end of the front hole and the installation of the clamping slide in the longitudinal direction of the body fixing frame.

[0037] Let m D represents the distance from the front end of the cabin top slide groove to the front end of the body fixing frame in the longitudinal direction of the body fixing frame.

[0038] Let n D represents the distance from the rear end of the cabin top slide groove to the front end of the body fixing frame in the longitudinal direction of the body fixing frame.

[0039] Let s D represents the adjustable sliding distance of the cabin top slide groove.

[0040] Set e as the reference standard, with the following parameter value ranges:

[0041] e The value range of D is 17-21mm;

[0042] d The value range of D is ;

[0043] f The value range of D is ;

[0044] g the value range of ;

[0045] w the value range of ;

[0046] k the value range of ;

[0047] m the value range of ;

[0048] n the value range of ;

[0049] s the value range of ;

[0050] The value range of θ is 30°-35°.

[0051] Preferably, the nuclear magnetic bed plate further comprises a mouthpiece, two head positioning holes, two body frame sliding grooves, and a heating groove hole.

[0052] The nuclear magnetic plate groove is arranged in the middle of the nuclear magnetic bed plate along the length direction of the nuclear magnetic bed plate. A mouthpiece, two head positioning holes, and two body frame sliding grooves are sequentially arranged on the first groove of the nuclear magnetic plate groove and in the direction to the second groove. The mouthpiece is inserted into the two inner side walls of the first groove at both ends. The two head positioning holes are arranged on the two side walls of the first groove. The two body frame sliding grooves are arranged on the two side walls of the first groove.

[0053] The heating groove hole is arranged at the position where the end of the heat preservation bin communicates with the side wall of the nuclear magnetic bed plate. The heating groove hole is arranged below the nuclear magnetic plate groove in space.

[0054] The mouthpiece further comprises a mouth hole which is a square through hole arranged in the middle of the main body of the mouthpiece.

[0055] Preferably, the bifurcated part of the neck clamping block is designed as a U-shaped neck clamping groove. One end of the neck clamping groove is connected with one end of a rod body structure. The main part of the rod body structure further comprises a neck clamping hole which is a through hole.

[0056] Preferably, the sheet part of the limiting plate is a pressing plate. The limiting plate further comprises a bottom nut, a top nut, a pressing plate stud, and a pressing plate sheet.

[0057] The pressing plate is located at the lowermost position of the limiting plate, and the pressing plate comprises a pressing plate stud and a pressing plate sheet, the pressing plate sheet is a circular arc sheet structure, and the pressing plate stud is vertically fixed at one end of the upper surface of the pressing plate sheet; the top nut and the bottom nut are sequentially sleeved on the pressing plate stud from top to bottom.

[0058] Preferably, the ventilation pipe is a hollow tubular structure, the side wall of the ventilation side is a flat structure, and two recessed groove channels are arranged on the side wall opposite to the flat structure along the length direction of the pipe body.

[0059] A ventilation clamping slot is arranged on the arc-shaped side wall between the flat part and the groove at the port of one end of the ventilation pipe.

[0060] Preferably, the inner surface of the body fixing frame is detachably attached to a layer of anti-skid layer, and the surface of the anti-skid layer has a group of dot matrix arranged convex points.

[0061] The present application has the following advantages:

[0062] 1. The experimental mouse is placed into the cabin port from the rear of the body fixing frame, so that the mouth and nose of the mouse protrude from the front hole, the body position of the mouse is adjusted through the lower hole, the four legs of the mouse are respectively stretched out from the four leg holes, and the legs are fixed on the outer surface of the body fixing frame by using adhesive tape after the joints of the mouse legs are bent, so that the four legs of the mouse have no force point and the mouse is prevented from struggling.

[0063] 2. The sliding block is aligned with the body frame sliding groove on the nuclear magnetic bed plate, the mouse is first loaded into the body fixing frame, and then the body fixing frame is loaded into the nuclear magnetic bed plate and assembled with the body frame sliding groove on the nuclear magnetic bed plate, and the disassembly process is opposite, so that the device has the advantages of convenient installation and disassembly.

[0064] 3. The occluder is used for mouse occlusion, the occlusion hole is used for sleeving and fixing the incisors of the mouse, and the head of the mouse is fixed. In addition, the ventilation clamping slot can be clamped on the occluder, and the internal device is not easy to move after assembly. Therefore, the overall structure of the device is more compact, and the rationality and strong stability of the structure layout are also reflected.

[0065] 4. When the nuclear magnetic bed plate is assembled with the second groove, the heat preservation bin is located below the second groove, and the electromagnetic heating block is internally provided with an electromagnetic coil for heating. In the experimental process, the electromagnetic heating block is started to heat and preserve the mouse in the body fixing frame from the upper and lower directions, so that the experimental mouse is in a more comfortable temperature, thereby ensuring that the body and posture of the mouse will not move, and the acquisition quality of the mouse brain magnetic resonance imaging is improved.

[0066] 5. When the mouse is installed in the body fixing frame, the neck of the mouse is below the clamping hole, at this time, the neck clamping groove of the neck clamping block is aligned with the position of the neck of the mouse, then the neck clamping block is adjusted up and down along the clamping sliding groove, the neck of the mouse is clamped in a relatively comfortable state, and then the relative position of the neck clamping block and the clamping sliding plate is fixed by using the bolt and the nut.

[0067] The pressing plate piece of the pressing plate is placed at the back waist of the mouse, the position and height of the pressing plate are adjusted, the mouse is in a relatively comfortable state, then the pressing plate is fixed by using the top nut and the bottom nut, and the back of the body of the mouse is fixed by using the limiting plate.

[0068] The body of the mouse is further fixed by the limiting plate and the neck clamping block, so that the probability of keeping the body and posture of the mouse in a stationary state is further improved. BRIEF DESCRIPTION OF DRAWINGS

[0069] Figure 1 It is the overall installation drawing involved in the present application;

[0070] Figure 2 It is the exploded view of the present application;

[0071] Figure 3 It is the structural schematic view of the nuclear magnetic bed plate involved in the present application;

[0072] Figure 4 It is the side rear view of the nuclear magnetic bed plate involved in the present application;

[0073] Figure 5 It is the side partial view of the nuclear magnetic bed plate involved in the present application;

[0074] Figure 6 It is the side rear view of the body fixing frame involved in the present application;

[0075] Figure 7 It is the front lower side view of the body fixing frame involved in the present application;

[0076] Figure 8 It is the bottom view of the body fixing frame involved in the present application;

[0077] Figure 9 It is the side top view of the body fixing frame involved in the present application;

[0078] Figure 10 It is the sectional view of the body fixing frame involved in the present application;

[0079] Figure 11 It is the structural schematic view of the neck clamping block involved in the present application;

[0080] Figure 12 It is the structural schematic view of the limiting plate involved in the present application;

[0081] Figure 13 This is an exploded view of the limiting plate involved in the present invention;

[0082] Figure 14 This is a schematic diagram of the ventilation tube involved in the present invention;

[0083] Figure 15 This is a top sectional view of the body fixation frame and auxiliary components involved in the present invention;

[0084] Figure 16 A cross-sectional view of the body fixation frame and auxiliary components involved in this invention.

[0085] Figure 17 This is a partial sectional view of the installation of the body fixation frame and auxiliary components involved in the present invention;

[0086] Figure 18 The key dimensions of the body fixation frame involved in this invention are proportions.

[0087] Figure 19 This invention relates to rsfMRI network diagrams of the brains of awake and anesthetized mice.

[0088] Figure 20 This invention relates to functional activation maps and imaging at different BOLD times in mice under anesthesia and wakefulness conditions following visual stimulation.

[0089] Figure 21 This is an illustration of the activation of the posterior spinal cord and infrathalamic complex under visual stimulation during anesthesia, as described in this invention.

[0090] Figure 22 This is an illustration of the existing mouse restraint device I according to the present invention;

[0091] Figure 23 This is an illustration of the existing mouse restraint device II according to the present invention;

[0092] Figure 24 This is an illustration of the existing mouse restraint device III according to the present invention;

[0093] Figure 25 This invention relates to a 9.4T small animal magnetic resonance imaging (MRI) system. Detailed Implementation Specific implementation method one:

[0095] This embodiment specifically describes a mouse body fixation clamp according to Embodiment 1, such as... Figure 1 and 2 As shown, the fixing fixture includes a nuclear magnetic bed plate 1, an electromagnetic heating block 2, an insulation box 3, an insulation pipe 4, a body fixing frame 5, a limiting plate 6, a neck clamp 7, and a ventilation pipe 8.

[0096] The NMR bed plate 1 has a left-right symmetrical structure. The inner side of the NMR bed plate 1 is provided with a NMR plate groove 1A. The length of the NMR plate groove 1A is adapted to the length of the NMR bed plate 1. The NMR plate groove 1A is divided into two sections, a first groove 9 and a second groove 10, according to the different widths between the two side walls.

[0097] The main body of the NMR bed plate 1 is equipped with a heat preservation chamber. When the NMR bed plate 1 is assembled with the second groove 10, the heat preservation chamber is located below the second groove 10, and the end of the heat preservation chamber is connected to the tail end face of the NMR bed plate 1.

[0098] The inner surface of the electromagnetic heating block 2 is provided with a third groove 11, and the end of the third groove 11 is connected to the side wall of the electromagnetic heating block 2; the width of the cross-section of the head end of the electromagnetic heating block 2 is greater than the width of the cross-section of the connecting end, and the electromagnetic heating block 2 is provided with an electromagnetic coil for heating inside.

[0099] The insulated box 3 has a box structure and is filled with heat-conducting liquid;

[0100] Insulation pipe 4 has a hollow tube structure, allowing heat-conducting liquid to flow through it;

[0101] The body fixation frame 5 has a hollow cylindrical structure; both ends of the body fixation frame 5 are open, and one end of the body fixation frame 5 tapers.

[0102] The limiting plate 6 is a screw structure with a plate-like part at one end;

[0103] The neck latch 7 is a rod structure with a forked portion;

[0104] Ventilation tube 8 is a hollow tube.

[0105] The MRI bed plate 1 is located at the bottom of the entire mechanism, and the heat preservation tube 4 is embedded in the heat preservation chamber inside the MRI bed plate 1; the body fixation frame 5 is slidably installed in the first groove 9, the front end of the body fixation frame 5 is connected to the ventilation tube 8, the upper front section of the body fixation frame 5 is fixed with a neck clamp 7, and the upper rear section of the body fixation frame 5 is equipped with a limit plate 6; electromagnetic heating blocks 2 and heat preservation boxes 3 are arranged from head to tail above the MRI bed plate 1, and the heat preservation box 3 is covered on top of the body fixation frame 5;

[0106] Among them, such as Figures 6-10 As shown, the body fixing frame 5 also includes a cabin opening 5A, a cabin top slide groove 5B, a snap-fit ​​sliding plate 5C, a snap-fit ​​hole 5D, two ear holes 5E, a half-shaft pin 5F, two slide blocks 5G, a front hole 5H, a lower hole 5I, and a snap-fit ​​slide groove 5C1.

[0107] The body fixation frame 5 is a hollow, symmetrical structure, and the main body of the body fixation frame 5 is a thin-walled shell structure;

[0108] The cabin port 5A is arranged at the rear end of the body fixing frame 5;

[0109] From the rear of the body fixing frame 5 to the front, the cabin top sliding groove 5B, the clamping sliding plate 5C, the clamping hole 5D, and the two ear holes 5E are sequentially arranged along the symmetry line on the top of the body fixing frame 5; the clamping sliding plate 5C is inserted on the hole edge of the clamping hole 5D close to the rear of the body fixing frame 5, and the two ear holes 5E are symmetrically arranged on the two sides of the body fixing frame 5;

[0110] The front hole 5H and the lower hole 5I are sequentially arranged from front to back along the symmetry line on the bottom of the body fixing frame 5 at the front end of the body fixing frame 5;

[0111] A half shaft pin 5F is arranged below each ear hole 5E at the front end of the body fixing frame 5;

[0112] A pair of left and right symmetric sliding groove blocks 5G are arranged on the bottom of the rear section of the body fixing frame 5, and the sliding groove blocks 5G are slidably inserted into the body frame sliding groove 1D on the nuclear magnetic bed plate 1 to form an integral body with the nuclear magnetic bed plate 1;

[0113] Among them,

[0114] A long hole clamping sliding groove 5C1 is arranged in the middle of the plate body of the clamping sliding plate 5C;

[0115] The cabin top sliding groove 5B, the clamping hole 5D, the two ear holes 5E, the front hole 5H, and the lower hole 5I are all through holes; the shape of the cabin top sliding groove 5B is rectangular;

[0116] Among them, the size parameters of each part of the body fixing frame 5 are set as follows:

[0117] Let d D represents the diameter of the front hole 5H, which cooperates with the mouth and nose of the mouse, and when the head of the mouse is inserted, the mouse can keep its mouth open, so that the mouse can bite the bite plate 1B with its front teeth, and the head of the mouse can be fixed;

[0118] Let θ represent the included angle of the upper and lower directions of the inner wall of the front hole 5H, and the angle inside the front hole 5H is designed to fit the head of the mouse, so that the mouse can maintain a relatively comfortable state for a long time;

[0119] Let e D represents the diameter of the mouse head, which is the distance from the front end of the clamping hole 5D to the front end of the lower hole 5I;

[0120] Let f D represents the diameter of the mouse neck, which is the diameter of the body fixing frame 5 on which the clamping sliding plate 5C is installed;

[0121] Let g D represents the diameter of the mouse trunk, which is the inner diameter of the cabin port 5A;

[0122] Let w denote the distance between the end of the mouse head and the front end of the body fixing frame, which is the distance between the front end of the front hole 5H and the middle of the clamping hole 5D in the longitudinal direction of the body fixing frame 5;

[0123] Let k denote the distance between the position of the maximum diameter behind the mouse neck and the front end of the body fixing frame, which is the distance between the front end of the front hole 5H and the installation position of the clamping sliding plate 5C in the longitudinal direction of the body fixing frame 5;

[0124] Let m denote the distance between the front end of the cabin top sliding groove 5B and the front end of the body fixing frame 5 in the longitudinal direction of the body fixing frame 5;

[0125] Let n denote the distance between the rear end of the cabin top sliding groove 5B and the front end of the body fixing frame 5 in the longitudinal direction of the body fixing frame 5;

[0126] Let s denote the adjustable sliding distance of the cabin top sliding groove 5B, which is used to adjust the limiting plate 6 to fix the mouse's torso;

[0127] According to the sampling measurement of the mouse body and the body state of the mouse during the experiment, according to the body proportion characteristics of the mouse, e the following parameter value ranges are set as reference benchmarks:

[0128] Actual measurement is performed on mice of 6-9 weeks;

[0129] e The value range of L is 17-21mm;

[0130] d The value range of L is ;

[0131] f The value range of L is ;

[0132] g The value range of L is ;

[0133] w The value range of L is ;

[0134] k The value range of L is ;

[0135] m The value range of L is ;

[0136] n The value range of L is ;

[0137] s The value range of θ is 30°-35°. ;

[0138] The value range of θ is 30°-35°.

[0139] The size of the body fixing frame 5 is designed according to the above ratio, so as to meet the fixing of the body of the mouse in the experiment and obtain better magnetic resonance imaging data. Specific embodiment two:

[0141] Different from the specific embodiment one, a mouse body fixing clamp in the embodiment, as shown in the structural schematic view of the nuclear magnetic bed plate shown in Figure 3 、 Figure 4 and Figure 5 , the nuclear magnetic bed plate 1 further comprises a mouthpiece 1B, two head positioning holes 1C, two body frame sliding grooves 1D, and a heating groove hole 1E.

[0142] The nuclear magnetic plate groove 1A is arranged in the middle of the length direction of the nuclear magnetic bed plate 1, and the mouthpiece 1B, the two head positioning holes 1C, and the two body frame sliding grooves 1D are sequentially arranged in the direction from the first groove 9 to the second groove 10 of the nuclear magnetic plate groove 1A; the mouthpiece 1B is inserted into the two inner side walls of the first groove 9, the two head positioning holes 1C are arranged on the two side walls of the first groove 9, and the two body frame sliding grooves 1D are arranged on the two side walls of the first groove 9.

[0143] The end of the heat preservation bin communicates with the side wall of the nuclear magnetic bed plate 1, and the heating groove hole 1E is located below the nuclear magnetic plate groove 1A in space.

[0144] The mouthpiece 1B further comprises a 1B1, and the occlusion hole 1B1 is located in the middle of the main body of the mouthpiece 1B and is a square through hole; the mouthpiece 1B is used for mouse occlusion, and the occlusion hole 1B1 is used for sleeving and fixing the incisors of the mouse, thereby realizing the fixation of the head of the mouse. Specific embodiment three:

[0146] Different from the specific embodiment one or two, a mouse body fixing clamp in the embodiment, as shown in Figure 11 , the bifurcated part of the neck clamping block 7 is designed as a U-shaped neck clamping groove 7B, one end of the neck clamping groove 7B is connected with the arc end of the rod body structure, and the main part of the rod body structure is further provided with a neck clamping hole 7A; the neck clamping hole 7A is a through hole. Specific embodiment four:

[0148] Different from the specific embodiment three, a mouse body fixing clamp in the embodiment, as shown in Figures 12-13As shown, the sheet-shaped part of the limiting plate 6 is selected as a pressing plate 6A, and the limiting plate 6 further comprises a bottom nut 6B, a top nut 6C, a pressing plate stud 6A1 and a pressing plate sheet 6A2;

[0149] The pressing plate 6A is located at the lowermost part of the limiting plate 6, and the pressing plate 6A comprises the pressing plate stud 6A1 and the pressing plate sheet 6A2. The pressing plate sheet 6A2 is a circular arc sheet structure, and the pressing plate stud 6A1 is vertically fixed at one end of the upper surface of the pressing plate sheet 6A2. The top nut 6C and the bottom nut 6B are sequentially sleeved on the pressing plate stud 6A1 from top to bottom through threads, and the positions of the top nut 6C and the bottom nut 6B on the pressing plate stud 6A1 can be adjusted by rotating them respectively. Specific implementation five

[0151] Different from the specific implementation four, a mouse body fixing clamp in the present implementation is as shown in the accompanying drawings, Figure 14 As shown, the ventilation pipe 8 is a hollow tubular structure, one side wall of the ventilation pipe 8 is a flat structure, and two recessed groove channels 8B are arranged on the side wall opposite to the flat structure along the length direction of the pipe body.

[0152] The ventilation pipe 8 is provided with a ventilation clamping slot 8A on the arc-shaped side wall between the flat part and the groove at the port of one end of the ventilation pipe 8, and the ventilation clamping slot 8A can be clamped on the occluder 1B. Specific implementation six

[0154] Different from the specific implementation five, a mouse body fixing clamp in the present implementation is as shown in the accompanying drawings,

[0155] Working principle

[0156] As shown in the accompanying drawings, Figures 15-18 In the limiting plate 6, the pressing plate sheet 6A2 of the pressing plate 6A is located in the cabin body port 5A of the body fixing frame 5, the pressing plate stud 6A1 of the pressing plate 6A is inserted into the cabin top sliding groove 5B, and the pressing plate stud 6A1 can reciprocally slide in the cabin top sliding groove 5B and can also move up and down, so as to adjust the position and height of the pressing plate 6A. The bottom nut 6B is sleeved on the pressing plate stud 6A1 through threads, and the bottom nut 6B is located in the cabin body port 5A and can be abutted against the inner side of the cabin top sliding groove 5B at the upper part of the cabin body port 5A. The top nut 6C is also sleeved on the pressing plate stud 6A1 through threads, and the top nut 6C is located at the outer side of the cabin top sliding groove 5B at the upper part of the cabin body port 5A. By rotating and adjusting the top nut 6C and the bottom nut 6B, the top nut 6C and the bottom nut 6B can be formed to be tightened from the inner and outer sides of the cabin top sliding groove 5B respectively, so as to fix the pressing plate 6A.

[0157] The neck clamping block 7 is placed on the front side of the clamping slide plate 5C, the neck clamping groove 7B of the neck clamping block is downwardly aligned with the clamping hole 5D, the neck clamping hole 7A is aligned with the clamping slide groove 5C1, the neck clamping block 7 can reciprocally slide along the clamping slide groove 5C1, after being adjusted to a proper position, the bolt is sequentially threaded into the neck clamping hole 7A and the clamping slide groove 5C1, then the nut is sleeved on the other end, and the relative position of the neck clamping block 7 and the clamping slide plate 5C is fixed by the bolt and the nut.

[0158] In use, first, the experimental mouse is placed into the cabin body opening 5A from the rear of the body fixing frame 5, the mouth and nose of the mouse are projected from the front hole 5H, and the ears of the mouse are projected from the two ear holes 5E.

[0159] At this time, the neck of the mouse is located below the clamping hole 5D, the neck clamping groove 7B of the neck clamping block 7 is aligned with the neck position of the mouse, then the neck clamping block 7 is adjusted to slide up and down along the clamping slide groove 5C1, the neck of the mouse is clamped in a relatively comfortable state, and then the relative position of the neck clamping block 7 and the clamping slide plate 5C is fixed by the bolt and the nut.

[0160] The pressing plate piece 6A2 of the pressing plate 6A is placed on the rear waist of the mouse, the position and height of the pressing plate 6A are adjusted, the mouse is in a relatively comfortable state, then the pressing plate 6A is fixed by the top nut 6C and the bottom nut 6B, and the rear part of the body of the mouse is fixed by the limiting plate 6.

[0161] The embodiments of the present application disclose the preferred embodiments, but are not limited thereto, and the ordinary skilled in the art can easily understand the spirit of the present application according to the above-mentioned embodiments, and make different inferences and changes, as long as they do not deviate from the spirit of the present application, and are within the protection scope of the present application.

Claims

1. A mouse body fixation clamp, characterized in that: The fixing fixture includes a nuclear magnetic bed plate (1), an electromagnetic heating block (2), an insulated box (3), an insulated pipe (4), a body fixing frame (5), a limiting plate (6), a neck clamp (7), and an air pipe (8); The nuclear magnetic resonance bed plate (1) has a left-right symmetrical structure. The inner side of the nuclear magnetic resonance bed plate (1) is provided with a nuclear magnetic resonance plate groove (1A). The length of the nuclear magnetic resonance plate groove (1A) is adapted to the length of the nuclear magnetic resonance bed plate (1). The nuclear magnetic resonance plate groove (1A) is divided into two sections: a first groove (9) and a second groove (10). The main body of the nuclear magnetic resonance bed plate (1) is equipped with a heat preservation chamber, and the end of the heat preservation chamber is connected to the tail end face of the nuclear magnetic resonance bed plate (1). The inner surface of the electromagnetic heating block (2) is provided with a third groove (11), and the end of the third groove (11) is connected to the side wall of the electromagnetic heating block (2); the width of the cross-section of the head end of the electromagnetic heating block (2) is greater than the width of the cross-section of the connecting end, and an electromagnetic coil is provided inside the electromagnetic heating block (2). The insulated box (3) is a box structure, and the inside is filled with heat-conducting liquid; The insulation pipe (4) has a hollow pipe structure; The body fixation frame (5) is a hollow cylindrical structure; both ends of the body fixation frame (5) are open structures, and one end of the body fixation frame (5) tapers. The limiting plate (6) is a screw structure with a plate-like part at one end; The neck clamp (7) is a rod structure with a forked part; The vent tube (8) is a hollow tube; The nuclear magnetic resonance bed plate (1) is located at the bottom of the whole set of mechanisms, and the heat preservation tube (4) is embedded in the heat preservation chamber inside the nuclear magnetic resonance bed plate (1); the body fixation frame (5) is slidably installed in the first groove (9), the front end of the body fixation frame (5) is connected to the ventilation tube (8), the upper part of the front section of the body fixation frame (5) is fixed with a neck clamp (7), and the upper part of the rear section of the body fixation frame (5) is equipped with a limit plate (6); the electromagnetic heating block (2) and the heat preservation box (3) are arranged from head to tail on the top of the nuclear magnetic resonance bed plate (1), and the heat preservation box (3) is covered on the top of the body fixation frame (5); The body fixing frame (5) also includes a cabin opening (5A), a cabin top slide groove (5B), a snap-fit ​​sliding plate (5C), a snap-fit ​​hole (5D), two ear holes (5E), a half shaft pin (5F), two slide blocks (5G), a front hole (5H), a lower hole (5I), and a snap-fit ​​slide groove (5C1). The body fixation frame (5) is a hollow, symmetrical structure, and the main body of the body fixation frame (5) is a thin-walled shell structure; The hatch (5A) is located at the rear end of the body fixation frame (5); From the rear to the front of the body fixation frame (5), along the symmetrical line at the top of the body fixation frame (5), a cabin top slide (5B), a snap-fit ​​slide plate (5C), a snap-fit ​​hole (5D), and two ear holes (5E) are arranged in sequence; and the snap-fit ​​slide plate (5C) is inserted into the edge of the hole near the rear of the body fixation frame (5D), and the two ear holes (5E) are symmetrically arranged on both sides of the body fixation frame (5); At the front end of the body fixation frame (5), a front hole (5H) and a lower hole (5I) are provided sequentially from front to back along the symmetrical line of the bottom of the body fixation frame (5). At the front end of the body fixation frame (5), a half-shaft pin (5F) is provided below each ear hole (5E). The rear bottom of the body fixation frame (5) is provided with a pair of two symmetrical sliding blocks (5G), which slide into the body frame sliding groove (1D) on the MRI bed plate (1); in, The snap-fit ​​slide plate (5C) has a long hole snap-fit ​​groove (5C1) in the middle of the plate body. The top slide (5B), snap-fit ​​hole (5D), two ear holes (5E), front hole (5H), and lower hole (5I) are all through holes; the top slide (5B) is rectangular in shape. The dimensional parameters of each component of the body fixation frame (5) are set as follows: set up d This indicates the diameter of the anterior aperture (5H). The diameter of the anterior aperture (5H) is matched with the mouth and nose of the mouse. When the mouse's head is inserted, it can ensure that the mouse's mouth is open so that the mouse's incisors can bite on the bite block (1B) to fix the mouse's head. Let θ represent the angle between the upper and lower directions of the inner wall of the front hole (5H) and the angle inside the front hole (5H) so that it fits the mouse's head and can keep the mouse in a relatively comfortable state for a long time. set up e The diameter of the end of the mouse head is the distance from the front end of the snap-fit ​​hole (5D) to the front end of the lower hole (5I). set up f The maximum diameter at the back of the mouse's neck is the diameter of the snap-on slide (5C) mounted on the body fixation frame (5); set up g This indicates the diameter of the mouse's trunk and the inner diameter of the hatch (5A). set up w The distance between the end of the mouse head and the front end of the body fixation frame is the distance between the front end of the front hole (5H) and the middle of the snap-fit ​​hole (5D) in the longitudinal direction of the body fixation frame (5); set up k The distance between the maximum diameter of the mouse's neck and the front end of the body fixation frame is the distance from the front end of the front hole (5H) to the mounting clip slide (5C) in the longitudinal direction of the body fixation frame (5). set up m This indicates the distance between the front end of the cabin roof slide (5B) and the frontmost end of the body fixation frame (5) in the longitudinal direction; set up n The distance between the rear end of the cabin top slide (5B) and the front end of the body fixation frame (5) in the longitudinal direction is indicated. set up s Indicates the adjustable sliding distance of the top slide (5B); Will e Set as the reference base, and set the following parameter value range: e The value range is 17-21 mm; d The range of values ​​is ; f The range of values ​​is ; g The range of values ​​is ; w The range of values ​​is ; k The range of values ​​is ; m The range of values ​​is ; n The range of values ​​is ; s The range of values ​​is ; The value of θ ranges from 30° to 35°.

2. The mouse body fixation clamp according to claim 1, characterized in that: The nuclear magnetic resonance bed plate (1) also includes a bite device (1B), two head positioning holes (1C), two body frame slides (1D), and a heating slot hole (1E). The MRI plate groove (1A) is located in the middle along the length of the MRI bed plate (1). On the first groove (9) of the MRI plate groove (1A) and in the direction to the second groove (10), there are sequentially provided a bite device (1B), two head positioning holes (1C) and two body frame slides (1D). The bite device (1B) is inserted into the two inner side walls of the first groove (9) at both ends. The two head positioning holes (1C) are correspondingly located on the two side walls of the first groove (9), and the two body frame slides (1D) are correspondingly located on the two side walls of the first groove (9). The end of the heat preservation chamber is connected to the side wall of the nuclear magnetic bed plate (1) at the end of the heat preservation chamber, which is a heating slot (1E); and the heating slot (1E) is located below the nuclear magnetic plate groove (1A) in space; The bite device (1B) also includes (1B1), and the bite hole (1B1) is located in the middle of the body of the bite device (1B) and is a square through hole.

3. A mouse body fixation clamp according to claim 2, characterized in that: The bifurcation of the neck block (7) is designed as a U-shaped neck groove (7B). The arc end of the neck groove (7B) is connected to one end of the rod structure. The main body of the rod structure is also provided with a neck hole (7A). The neck hole (7A) is a through hole.

4. A mouse body fixation clamp according to claim 3, characterized in that: The sheet-like part of the limiting plate (6) is selected as a pressure plate (6A), and the limiting plate (6) also includes a bottom nut (6B), a top nut (6C), a pressure plate stud (6A1), and a pressure plate sheet (6A2). The pressure plate (6A) is located at the bottom of the limiting plate (6). The pressure plate (6A) includes a pressure plate stud (6A1) and a pressure plate sheet (6A2). The pressure plate sheet (6A2) is an arc-shaped thin sheet structure. The pressure plate stud (6A1) is vertically fixed to one end of the upper surface of the pressure plate sheet (6A2). The top nut (6C) and the bottom nut (6B) are sequentially fitted onto the pressure plate stud (6A1) from top to bottom.

5. A mouse body fixation clamp according to claim 4, characterized in that: The ventilation tube (8) is a hollow tubular structure. One side wall of the ventilation tube (8) is a straight structure. On the side wall of the ventilation tube (8) opposite to the straight structure, two recessed channels (8B) are provided along the length of the tube. At one end of the ventilator (8), and on the arc-shaped sidewall between the straight part and the groove, there is a ventilator slot (8A), which engages with the bite device (1B).

6. A mouse body fixation clamp according to claim 5, characterized in that: The inner surface of the body fixation frame (5) is detachably attached with an anti-slip layer, the surface of which has a set of dot matrix-arranged protrusions.

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