A method for improving the stability of bird's nest acid aqueous solution

Abstract

The present invention provides a method for improving the stability of a bird's nest acid aqueous solution, the method for improving the stability of a bird's nest acid aqueous solution comprising the step of adding a stabilizer to the bird's nest acid aqueous solution, wherein the stabilizer is one or more of a sulfite and a bisulfite. The present invention effectively suppresses the loss and discoloration of bird's nest acid by adding a sulfite and / or a bisulfite to the bird's nest acid aqueous solution, especially under the condition of low pH value and high bird's nest acid content, the effect is significantly better than the prior art, and provides technical support for adding bird's nest acid to cosmetics, broadening the application scope of bird's nest acid.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a method for improving the stability of a bird's nest acid aqueous solution. Background Art

[0002] Bird's nest acid, also known as sialic acid and N-acetylneuraminic acid, is a 9-carbon monosaccharide derivative. It is widely distributed in human tissues, saliva, brain, and amniotic fluid, with the highest content in cerebrospinal fluid and breast milk colostrum. Bird's nest acid is also widely distributed in nature, and is rich in dairy products, eggs, and bird's nests. Numerous scientific studies have shown that bird's nest acid has multiple biological functions: 1. Promote brain intelligence development; 2. Whiten skin; 3. Anti-aging; 4. Anti-inflammatory and anti-allergic; 5. Antiviral, etc. Therefore, bird's nest acid is widely used in health products, food, cosmetics, and medicines.

[0003] Due to the process and the structure of bird's nest acid itself, the bird's nest acid aqueous solution will have a certain amount of content loss during processing and storage, and the aqueous solution will change color, affecting the product quality. In previous studies, the applicant found that the use of pH adjustment and the addition of potassium sorbate to the bird's nest acid aqueous solution can improve the content loss and discoloration of the bird's nest acid aqueous solution to a certain extent. However, the mechanism of bird's nest acid loss and discoloration is very complicated, and the above-mentioned improvement method also has limitations in application and effect. For example, the amount of bird's nest acid added to it is relatively low. When the bird's nest acid content is high (≥2%), the improvement effect is not significant. With the deepening of bird's nest acid functional research, the use content will continue to expand; the applicable pH range is very narrow, and in the field of cosmetics, the pH range spans a large range, generally between 3.5 and 8.5, and some products containing α-hydroxy acids may have a pH value between 3-4. Therefore, it is necessary to further enrich the solution to this problem. Summary of the invention

[0004] The present invention provides a method for improving the stability of a bird's nest acid aqueous solution, so as to solve the defects of the bird's nest acid aqueous solution in the prior art that is easy to change color and reduce the content, especially the loss and discoloration under the conditions of low pH value and high bird's nest acid content, so as to realize the smooth addition of bird's nest acid in the cosmetics field.

[0005] The present invention provides a method for improving the stability of a bird's nest acid aqueous solution, comprising the step of adding a stabilizer to the bird's nest acid aqueous solution, wherein the stabilizer is one or more of a sulfite and a bisulfite.

[0006] The present invention has found that adding one or more of sulfites and bisulfites to the aqueous solution of bird's nest acid can effectively inhibit the loss and discoloration of bird's nest acid, especially under low pH values ​​and high bird's nest acid content (greater than or equal to 2%). The effect is significantly better than existing technologies such as adding potassium sorbate, thereby providing technical support for adding bird's nest acid in cosmetics.

[0007] It should be noted that sulfites, bisulfites and potassium sorbate can be used as preservatives. However, this series of substances also has an antioxidant function, but this does not mean that adding antioxidants or a combination of potassium sorbate and antioxidants to the bird's nest acid aqueous solution can solve the problem of discoloration and loss of bird's nest acid. For example, the use of antioxidants such as glucosylrutin and glutathione cannot achieve a good effect.

[0008] In addition, the present invention has also tried other agents, such as zinc sulfate, phenoxyethanol, and p-hydroxyacetophenone, which also failed to achieve the effect of the present invention. Among them, zinc sulfate and p-hydroxyacetophenone are often used as antibacterial agents, and phenoxyethanol is a common preservative like potassium sorbate.

[0009] It can be seen that not all substances with antiseptic function or substances with antiseptic and antioxidant functions can effectively solve the defects of easy discoloration and content reduction of aqueous solution of bird's nest acid in the prior art, especially the loss and discoloration under low pH value and high bird's nest acid content. The mechanism is complicated and no clear explanation can be given for the time being. The present invention is also an unexpected discovery. The excellent effect of preventing discoloration and loss has really exceeded the expectations of the inventors.

[0010] In addition, it should be noted that in the prior art, the use of sulfite is mentioned in the detection experiment of sialic acid in dairy products. In order to distinguish sialic acid from lactose in dairy products, sialic acid needs to be derivatized to produce color. Sulfite / bisulfite is added as an antioxidant during the reaction. In order to prevent the introduction of interfering substances by self-oxidation of derivative reagents such as o-phenylenediamine, the antioxidant effect of sulfite / bisulfite is actually exerted. The same effect can be achieved by replacing other antioxidants in this method, and it has no effect on preventing the discoloration and attenuation of bird's nest acid.

[0011] According to the method for improving the stability of the swallow's nest acid aqueous solution provided by the present invention, the mass fraction of swallow's nest acid in the swallow's nest acid aqueous solution is 0.01-5%.

[0012] The present invention is applicable to the improvement method of bird's nest acid with a mass fraction of 0.01-5%, which can completely cover the situation of adding bird's nest acid into cosmetics.

[0013] According to the method for improving the stability of the aqueous solution of bird's nest acid provided by the present invention, the pH value of the aqueous solution of bird's nest acid is in the range of 3-8.5.

[0014] As mentioned in the background technology section, in the cosmetics field, the pH range is relatively wide, generally between 3.5 and 8.5, and some products containing α-hydroxy acids may be lower (about 3-4). The method of adding sulfite and bisulfite series substances of the present invention is fully applicable to the above pH range.

[0015] The bird's nest acid in the present invention can be purchased commercially, or can be produced by fermentation, enzymatic methods, etc. The bird's nest acid in the specific embodiment of the present invention is prepared by the inventor according to the preparation method disclosed in the Chinese patent application with application number 201811372409.0.

[0016] According to the method for improving the stability of the aqueous solution of bird's nest acid provided by the present invention, the amount of the stabilizer added is 0.03-0.1% of the mass of the aqueous solution of bird's nest acid.

[0017] The present invention adds sulfite and bisulfite series substances, which not only has a good effect of inhibiting the discoloration and loss of bird's nest acid, but also the added amount is less than the case of using potassium sorbate.

[0018] According to the method for improving the stability of the aqueous solution of bird's nest acid provided by the present invention, the sulfite is selected from one or more of sodium sulfite, potassium sulfite, ammonium sulfite, sodium pyrosulfite and potassium pyrosulfite.

[0019] According to the method for improving the stability of the aqueous solution of bird's nest acid provided by the present invention, the bisulfite is selected from one or more of sodium bisulfite, potassium bisulfite, and ammonium bisulfite.

[0020] According to the method for improving the stability of the bird's nest acid aqueous solution provided by the present invention, after the improvement, the bird's nest acid aqueous solution does not change color during processing and storage, and the bird's nest acid loss rate does not exceed 1%.

[0021] The present invention also provides application of the method for improving the stability of a bird's nest acid aqueous solution in the preparation of cosmetics containing bird's nest acid.

[0022] The present invention also provides a stable bird's nest acid aqueous solution composition, which is composed of a bird's nest acid aqueous solution and one or more of sulfites and bisulfites. The composition has stable properties and is not prone to discoloration or activity loss.

[0023] The method for improving the stability of a bird's nest acid aqueous solution provided by the present invention effectively suppresses the loss and discoloration of bird's nest acid by adding one or more of sulfites and bisulfites to the bird's nest acid aqueous solution. Especially under the conditions of low pH value and high bird's nest acid content, the effect is significantly better than that of the prior art, providing technical support for adding bird's nest acid to cosmetics and broadening the application scope of bird's nest acid. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] The concentrations involved in the following examples are all mass percentage concentrations, w / w%, unless otherwise specified.

[0026] The method for determining the content of scutellaria baicalensis was high performance liquid chromatography (HPLC), and the specific test conditions were as follows: Shimadzu Lc-15c; detection column Bio-Rad AMINEX HPX 87HOrganic Analysis Column (300×7.8mm); column temperature 60°C; mobile phase 6mmol sulfuric acid, flow rate 0.6ml / min; detection wavelength 210nm. All experiments were repeated 3 times, and the experimental results were the average of 3 times.

[0027] Experimental example

[0028] A certain amount of bird's nest acid was weighed and added to ultrapure water to prepare bird's nest acid aqueous solutions of different concentrations, which also contained 0.5% phenoxyethanol (preservative), triethanolamine and citric acid to adjust the pH to 3.8±0.1.

[0029] Take the above-mentioned bird's nest acid aqueous solution, add one or more of sodium bisulfite, sodium pyrosulfite, and sodium sulfite of different concentrations, as shown in Table 1, and different comparative examples, as shown in Table 2, and then treat at 85°C for 1h (to meet the raw material processing requirements in the cosmetics field), and then place at 45°C for 2 months. The color change of each group and the bird's nest acid loss rate are shown in Tables 1 and 2.

[0030] Table 1

[0031]

[0032] Note: The “-” in the table is due to the measurement error showing that the content of bird's nest acid has increased, but the error is within the allowable range, that is, the negative bird's nest acid loss rate means that there is no loss of bird's nest acid.

[0033] Table 2

[0034]

[0035]

[0036] In addition, a sample solution was prepared according to Example 1 and Comparative Example 2, but the difference was that it was heated at 121° C. for 20 minutes and then placed at 45° C. for 2 months. The results are shown in the following table.

[0037] Table 3

[0038]

[0039] From the above results, it can be seen that adding 0.03-0.1% of one or more of bisulfite and sulfite to the aqueous solution of bird's nest acid can effectively improve the problems of bird's nest acid loss and discoloration, especially under low pH values ​​(3-4) and high bird's nest acid content (≥2%). The effect is significantly better than potassium sorbate, glucosylrutin, zinc sulfate and other substances.

[0040] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for improving the stability of a bird's nest acid aqueous solution, characterized in that: The method comprises the step of adding a stabilizer to the aqueous solution of the bird's nest acid, wherein the stabilizer is one or more of sulfites and bisulfites; after the improvement, the aqueous solution of the bird's nest acid does not change color during processing and storage, and the loss rate of the bird's nest acid does not exceed 1%.

2. The method for improving the stability of the aqueous solution of bird's nest acid according to claim 1, characterized in that: The mass fraction of the bird's nest acid in the bird's nest acid aqueous solution is 0.01-5%.

3. The method for improving the stability of the aqueous solution of bird's nest acid according to claim 2, characterized in that: The pH value of the bird's nest acid aqueous solution is in the range of 3-8.

5.

4. The method for improving the stability of the aqueous solution of bird's nest acid according to claim 3, characterized in that: The pH value of the bird's nest acid aqueous solution is in the range of 3-4.

5. The method for improving the stability of a bird's nest acid aqueous solution according to any one of claims 1 to 4, characterized in that: The amount of the stabilizer added is 0.03-0.1% of the mass of the bird's nest acid aqueous solution.

6. The method for improving the stability of a bird's nest acid aqueous solution according to claim 1, characterized in that: The sulfite is selected from one or more of sodium sulfite, potassium sulfite, ammonium sulfite, sodium pyrosulfite and potassium pyrosulfite.

7. The method for improving the stability of a bird's nest acid aqueous solution according to claim 1, characterized in that: The bisulfite is selected from one or more of sodium bisulfite, potassium bisulfite and ammonium bisulfite.

8. Use of the method for improving the stability of a bird's nest acid aqueous solution according to any one of claims 1 to 7 in the preparation of cosmetics containing bird's nest acid.

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