Application of fingerprint spectrum and multi-component analysis of Radix Psoraleae in quality control

Abstract

The present invention relates to the fingerprint spectrum of Dapanlongqi medicinal material and the application of multi-component analysis in its quality control. The HPLC fingerprint spectrum of the Dapanlongqi medicinal material is basically consistent with Figure 1, with 14 characteristic fingerprint chromatographic peaks, wherein peak 1 is arbutin, peak 2 is gallic acid, peak 3 is bergenin, peak 5 is catechin, peak 7 is 4-O-galloyl bergenin, peak 9 is 11-O-galloyl bergenin, and peak 12 is catechin gallate.

Description

Technical Field

[0001] The invention belongs to the field of Chinese medicinal materials, and particularly relates to the application of fingerprint spectrum of Dapanlongqi medicinal material and multi-component analysis in its quality control. Background Art

[0002] Bergeniae Scopulosae Rhizoma is the dried rhizome of Bergenia scopulosa TPWang, a plant of the genus Bergenia in the Saxifragaceae family. It is one of the famous "Seven Medicines" of Taibai, mainly distributed in the Qinling Mountains and Qilian Mountains in Shaanxi Province. It is mainly used in the treatment of diarrhea, dysentery, metrorrhagia, leukorrhea, abdominal distension and pain, impetigo, alopecia, scabies and other diseases. Because the "Seven Medicines" have been passed down for many years in a way of habitual inheritance and oral transmission from teachers, the sources of Bergeniae Scopulosae used by different regions and ethnic groups are different. Bergeniae Scopulosae is used clinically as one of the sources of Bergeniae Scopulosae. Bergeniae Scopulosae has been used as Bergeniae Scopulosae in Guanzhong, Shaanxi (including Zhouzhi County, Lantian County, Mei County, Shaanxi Province, etc.).

[0003] At present, the chemical components separated and identified from Dapanlongqi mainly include isocoumarins, flavonoids, phenolic acids, anthraquinones, oxygen glycosides, carbon glycosides, etc. At present, there are few reports on the quality standards and content determination of Dapanlongqi medicinal materials, and there is no construction of medicinal material fingerprints and component identification. Fingerprints, as a quality control mode of traditional Chinese medicine, can be combined with chemical pattern recognition analysis to effectively analyze the components of traditional Chinese medicine, and combined with multi-component thin layer identification and multi-component content determination to further achieve the purpose of traditional Chinese medicine quality evaluation. Therefore, the present invention studies the fingerprint of Dapanlongqi medicinal materials for the first time, and establishes a method for simultaneously determining the contents of 7 components in Dapanlongqi, including arbutin, gallic acid, bergenin, catechin, 4-O-galloyl bergenin, 11-O-galloyl bergenin, and catechin gallate, for the first time, providing a basis for the overall quality evaluation of Dapanlongqi medicinal materials, the "Seven Medicines" of Taibai. Summary of the invention

[0004] The present invention provides an HPLC fingerprint of Dapanlongqi medicinal material, which is characterized in that when the HPLC chromatographic conditions are as follows, the HPLC fingerprint of Dapanlongqi medicinal material is basically the same as Figure 1 Consistency;

[0005] HPLC chromatographic conditions are as follows:

[0006] The chromatographic column is Agilent 5TC-C 18 Chromatographic column, specification: 250mm×4.6mm, 5μm;

[0007] Mobile phase: acetonitrile as phase A, 0.1% phosphoric acid aqueous solution as phase B;

[0008] Gradient elution program

[0009]

[0010]

[0011] Flow rate: 1.0 mL min -1 ; Column temperature: 25℃; Detection wavelength: 275nm; Injection volume: 10μL.

[0012] Another embodiment of the present invention provides the HPLC fingerprint of the above-mentioned Dapanlongqi medicinal material, characterized in that the HPLC fingerprint of the Dapanlongqi medicinal material is substantially the same as Figure 1 The results of the present invention are consistent with those of the above three compounds, with 14 characteristic fingerprint chromatographic peaks, of which peak 1 is arbutin, peak 2 is gallic acid, peak 3 is bergenin, peak 5 is catechin, peak 7 is 4-O-galloylbergenin, peak 9 is 11-O-galloylbergenin, and peak 12 is catechin gallate.

[0013] Another embodiment of the present invention provides the application of the HPLC fingerprint of the above-mentioned Dapanlongqi medicinal material in the quality control and component analysis of Dapanlongqi medicinal material.

[0014] Another embodiment of the present invention provides an application of the HPLC fingerprint of the above-mentioned Dapanlongqi medicinal material in the quality control of Dapanlongqi medicinal material, characterized in that the application comprises the following steps:

[0015] (1) Crush the Radix Panax Notoginseng, add methanol solution, perform ultrasonic treatment, and filter with a microporous membrane to obtain a test solution;

[0016] (2) Take the test sample solution obtained in step (1) and perform HPLC detection to obtain an HPLC chart of the test sample, wherein the chromatographic conditions are as follows:

[0017] The chromatographic column is Agilent 5TC-C 18 Chromatographic column, specification: 250mm×4.6mm, 5μm;

[0018] Mobile phase: acetonitrile as phase A, 0.1% phosphoric acid aqueous solution as phase B;

[0019] Gradient elution program

[0020]

[0021] Flow rate: 1.0 mL min -1 ; Column temperature: 25℃; Detection wavelength: 275nm; Injection volume: 10μL

[0022] (3) Compare the HPLC chromatogram of the test sample obtained in step (2) with the HPLC fingerprint of the Panax notoginseng medicinal material described in the present invention. The Panax notoginseng medicinal material with a similarity of more than 0.92 is a qualified product.

[0023] In step (1), preferably, the mixture is crushed and then sieved with a 60-mesh sieve, the methanol solution is preferably a methanol solution with a volume fraction of 60%, the ultrasonic time is preferably 20-40 min, and the microporous filter membrane is preferably a 0.22 μm filter membrane or a 0.45 μm filter membrane. Preferably, 0.1 g of the Dapanlongqi medicinal material sample powder (sieved with a 60-mesh sieve) is accurately weighed and placed in a 100 mL stoppered conical flask, 30 mL of 60% methanol is accurately added, the mass is weighed, and ultrasonic treatment is performed for 30 min, cooled, and the weight loss is supplemented with 60% methanol, and filtered through a 0.45 μm microporous filter membrane to obtain the obtained product.

[0024] Another embodiment of the present invention provides a method for analyzing the components of Dapanlongqi medicinal material, characterized in that the method comprises the following steps:

[0025] (1) Preparation of mixed solutions of 7 reference substances:

[0026] Take appropriate amount of arbutin, gallic acid, bergenin, catechin, 4-O-galloylbergenin, 11-O-galloylbergenin, and catechin gallate reference substances, accurately weigh them, and add 60% methanol aqueous solution to prepare mixed reference substance solutions with mass concentrations of 530, 150, 520, 300, 96, 110, and 103 μg / mL, respectively;

[0027] (2) Preparation of standard curves for each reference substance in step (1):

[0028] Accurately measure 0.1, 0.5, 1.0, 5.0, and 10.0 mL of the mixed reference solution respectively into a 10 mL volumetric flask, add 60% methanol to dilute to the scale, filter with a 0.45 μm microporous filter membrane, and perform HPLC detection. The mass concentration (x, μg / mL) of each reference substance is used as the abscissa, and the peak area value (y) is used as the ordinate. The linear equation is obtained by calculation, as shown in the following table:

[0029] Linear relationships among the seven components

[0030]

[0031] (3) Preparation of the test solution: crush the Radix Panax Psoraleae Radix, add methanol solution, perform ultrasonic treatment, and filter with a microporous membrane to obtain the test solution;

[0032] (4) taking the test solution obtained in step (3), detecting it by HPLC, and calculating the contents of arbutin, gallic acid, bergenin, catechin, 4-O-galloyl bergenin, 11-O-galloyl bergenin, and catechin gallate in the Radix Pseudostellariae according to the concentrations of arbutin, gallic acid, bergenin, catechin, 4-O-galloyl bergenin, 11-O-galloyl bergenin, and catechin gallate reference substances and their retention times in the fingerprint spectrum and the linear equation in step (2);

[0033] The chromatographic conditions of the HPLC detection in steps (2) and (4) are as follows:

[0034] The chromatographic column is Agilent 5TC-C 18 Chromatographic column, specification: 250mm×4.6mm, 5μm;

[0035] Mobile phase: acetonitrile as phase A, 0.1% phosphoric acid aqueous solution as phase B;

[0036] Gradient elution program

[0037]

[0038] Flow rate: 1.0 mL min -1 ; Column temperature: 25℃; Detection wavelength: 275nm; Injection volume: 10μL.

[0039] In step (3), preferably, the powder is crushed and then passed through a 60-mesh sieve, the methanol solution is preferably a methanol solution with a volume fraction of 60%, the ultrasonic time is preferably 20-40 min, and the microporous filter membrane is preferably a 0.22 μm filter membrane or a 0.45 μm filter membrane. Preferably, 0.1 g of the Dapanlongqi medicinal material sample powder (passed through a 60-mesh sieve) is accurately weighed and placed in a 100 mL stoppered conical flask, 30 mL of 60% methanol is accurately added, the mass is weighed, and ultrasonic treatment is performed for 30 min, cooled, and 60% methanol is used to make up for the weight loss, and filtered through a 0.45 μm microporous filter membrane to obtain.

[0040] Compared with the prior art, the advantages of the present invention are: (1) The present invention makes up for the deficiencies of the prior art, establishes a high performance liquid chromatography (HPLC) fingerprint of Dapanlongqi medicinal material, and provides an effective method for quality control of Dapanlongqi medicinal material; (2) The present invention matches the chromatograms of 10 batches of Dapanlongqi medicinal material samples to obtain a control spectrum. In the 10 batches of samples, a total of 14 chromatographic peaks are determined to be the common peaks of the fingerprint spectrum of Dapanlongqi medicinal material, and the common mode of the HPLC fingerprint spectrum of Dapanlongqi medicinal material is determined; (3) The HPLC fingerprint spectrum method and content determination method of Dapanlongqi medicinal material established by the present invention have good analytical evaluation capabilities, and have the advantages of accuracy, simplicity, stability and reliability. Therefore, the method can be used as an effective evaluation method for the quality of this medicinal material. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 It is the fingerprint of the Dapanlongqi medicinal material;

[0042] Figure 2 It is the fingerprint of 10 batches of Dapanlongqi medicinal materials;

[0043] Figure 3 This is the HPLC spectrum of a mixed reference substance containing 7 ingredients. DETAILED DESCRIPTION

[0044] Example 1

[0045] 1. Instruments and reagents

[0046] 1.1 Instrument

[0047] Agilent 1260 series high performance liquid chromatograph, equipped with VWD detector and Agilent Chem Station workstation (Agilent Technologies, Inc., USA); CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd., model: KQ-400DE); SQP type 1 / 100,000 electronic balance and SQP type 1 / 10,000 electronic balance (Sartorius Scientific Instrument (Beijing) Co., Ltd.).

[0048] 1.2 Drug testing

[0049] Arbutin (batch number: 18061203), bergenin (batch number: 17061307), and catechin (batch number: 18061201) reference substances were purchased from Shanghai Qiming Biological Co., Ltd., with a purity of ≥98%. Gallic acid reference substances were homemade in the laboratory, and the purity was ≥98% as determined by HPLC. 4-O-galloylbergenin (batch number: 191025, purity: 97.10%), 11-O-galloylbergenin (batch number: 22021402, purity: 97.47%), and catechin gallate (batch number: 21110401, purity: 98.72%) reference substances were purchased from Chengdu Pufeide Biotechnology Co., Ltd. Acetonitrile was chromatographically pure (Fisher Chemical Reagent Company), water was ultrapure water, and other reagents were analytically pure.

[0050] The medicinal materials of Dapanlongqi used in this experiment were collected in Shaanxi and Hebei, and were identified as the rhizomes of Qinling rock cabbage B. scopulosa TPWang by Associate Researcher Chen Zhiyong of Shaanxi Institute of Traditional Chinese Medicine. The sources of Dapanlongqi samples are shown in Table 1.

[0051] Table 1 Summary of Qinling Bergenia medicinal material sample information

[0052]

[0053] 2 Establishment of fingerprint

[0054] 2.2.1 Chromatographic conditions

[0055] Agilent 5TC-C 18 Chromatographic column (250mm×4.6mm, 5μm); acetonitrile (A)-0.1% phosphoric acid aqueous solution (B) as mobile phase, gradient elution (0-12min, 2% A; 12-14min, 2-11% A; 14-40min, 11-12% A; 40-85min, 12-24% A; 85-90min, 24% A), flow rate of 1.0mL / min, detection wavelength of 275nm; column temperature of 25℃; injection volume of 10μL.

[0056] 2.2.2 Preparation of test solution

[0057] Take the powder of the Dapanlongqi medicinal material sample (pass through a 60-mesh sieve), accurately weigh 0.1 g, place it in a 100-mL stoppered conical flask, accurately add 30 mL of 60% methanol, weigh the mass, ultrasonically treat for 30 minutes, cool, make up for the weight loss with 60% methanol, and filter with a 0.45 μm microporous filter membrane to obtain.

[0058] 2.2.3 Preparation of mixed reference solution I

[0059] Take appropriate amounts of arbutin, gallic acid, bergenin, catechin, 4-O-galloylbergenin, 11-O-galloylbergenin and catechin gallate reference substances, accurately weigh them, dissolve them in 60% methanol aqueous solution, and prepare mixed reference substance solutions with mass concentrations of 265, 75, 260, 150, 48, 55, and 51.5 μg / mL, respectively.

[0060] 2.2.4 Precision test

[0061] Take the same sample solution under "2.2.2", and measure it according to the chromatographic conditions under "2.2.1", and continuously inject and measure 6 times. Because the peak separation and symmetry of bergenin are good, the chromatographic response value is high, and it is cheap and easy to obtain, the retention time and peak area of ​​the bergenin peak are used as reference to calculate the relative retention time and relative peak area of ​​each common peak. As a result, the RSD value of the relative retention time of the 14 common peaks is 0.15% to 4.75% (n = 6), and the RSD value of the relative peak area is 0.20% to 3.83% (n = 6), indicating that this method has good precision.

[0062] 2.2.5 Stability test

[0063] Take the same sample solution under "2.2.2" and measure it according to the chromatographic conditions under "2.2.1" at 0, 4, 8, 12, 24, and 48 hours respectively. Take the retention time and peak area of ​​the bergenin peak as reference to calculate the relative retention time and relative peak area of ​​each common peak. As a result, the RSD value of the relative retention time of the 14 common peaks was 0.31% to 4.88% (n = 6), and the RSD value of the relative peak area was 0.23% to 3.69% (n = 6), indicating that the sample solution was stable within 48 hours.

[0064] 2.2.6 Repeatability test

[0065] Take the same batch of medicinal materials samples, prepare the test solution according to the method under "2.2.2", prepare 6 copies in parallel, and determine according to the chromatographic conditions under "2.2.1". Take the retention time and peak area of ​​the bergenin peak as reference to calculate the relative retention time and relative peak area of ​​each common peak. As a result, the RSD of the relative retention time of the 14 common peaks is 0.18% to 1.41% (n = 6), and the RSD value of the relative peak area is 1.11% to 4.21% (n = 6), indicating that this method has good repeatability.

[0066] 2.2.7 Establishment of HPLC fingerprint

[0067] Take 10 batches of Dapanlongqi medicinal material samples, prepare the test solution according to the method under "2.2.2", and then inject and measure according to the chromatographic conditions under "2.2.1", record the chromatographic data, and import it into the Chinese medicine chromatographic fingerprint similarity evaluation system (2012 version) for data analysis and processing. The chromatogram of the S1 sample was selected as the reference spectrum, and the multi-point correction method was used to generate the HPLC fingerprint of 10 batches of Dapanlongqi medicinal material samples using the median method ( Figure 2 ) and the control fingerprint ( Figure 1 ). Through the analysis of the fingerprint spectra of 10 batches of samples, it was found that there were 14 peaks in the figure that were common chromatographic peaks in the chromatograms of all samples. Therefore, it was determined that these 14 chromatographic peaks were the common peaks of Dapanlongqi medicinal materials.

[0068] 2.2.8 Similarity Evaluation

[0069] The similarity evaluation system of Chinese medicine chromatographic fingerprint (2012 version) was used to analyze the similarity of 10 batches of Dapanlongqi medicinal materials, as shown in Table 2. The results showed that the similarities of S1-S4 and S6-S10 were all greater than 0.920, indicating that the quality of these 9 batches of medicinal materials was relatively stable, but there were large differences in the content of each component; while the similarity of S5 sample was 0.752, indicating that S5 was quite different from other batches of medicinal materials, which may be related to factors such as origin, growth years, harvest time, and processing methods, and was an unqualified product.

[0070] Table 2 Similarity evaluation results

[0071]

[0072] 2.2.9 Common peak identification

[0073] By comparing the common peak with the mixed reference solution Ⅰ ( Figure 3 ) were compared with the retention times of the other common peaks, and 7 common peaks were identified, namely Peak 1 (arbutin), Peak 2 (gallic acid), Peak 3 (bergenin), Peak 5 (catechin), Peak 7 (4-O-galloylbergenin), Peak 9 (11-O-galloylbergenin) and Peak 12 (catechin gallate). Among them, Peak 3 (bergenin) had good separation and symmetry, high chromatographic response value, and was cheap and easy to obtain. Therefore, Peak 3 (bergenin) was selected as the reference peak (S) to calculate the relative retention times and relative peak areas of other common peaks.

[0074] Example 2

[0075] 2.4 Determination of multi-component content

[0076] 2.4.1 Chromatographic conditions

[0077] Agilent 5TC-C 18 Chromatographic column (250mm×4.6mm, 5μm); acetonitrile (A)-0.1% phosphoric acid aqueous solution (B) as mobile phase, gradient elution (0-12min, 2% A; 12-14min, 2-11% A; 14-40min, 11-12% A; 40-85min, 12-24% A; 85-90min, 24% A), flow rate of 1.0mL / min, detection wavelength of 275nm; column temperature of 25℃; injection volume of 10μL.

[0078] 2.4.2 Preparation of test solution

[0079] Take the powder of the Dapanlongqi medicinal material sample (pass through a 60-mesh sieve), accurately weigh 0.1 g, place it in a 100-mL stoppered conical flask, accurately add 30 mL of 60% methanol, weigh the mass, ultrasonically treat for 30 minutes, cool, make up for the weight loss with 60% methanol, and filter with a 0.45 μm microporous filter membrane to obtain.

[0080] 2.4.3 Preparation of mixed reference solution II

[0081] Take appropriate amount of arbutin, gallic acid, bergenin, catechin, 4-O-galloylbergenin, 11-O-galloylbergenin, and catechin gallate reference substances, accurately weigh them, and add 60% methanol aqueous solution to prepare mixed reference substance solutions with mass concentrations of 530, 150, 520, 300, 96, 110, and 103 μg / mL, respectively.

[0082] 2.4.4 Linear relationship study

[0083] Accurately pipette 0.1, 0.5, 1.0, 5.0, 10.0 mL of mixed reference solution II into a 10 mL volumetric flask, dilute to scale with 60% methanol, shake well, analyze according to the chromatographic conditions under "2.4.1", record the chromatogram, and determine the peak area. With the peak area as the ordinate and the reference concentration as the abscissa, the linear regression equation is obtained, see Table 3.

[0084] Table 3 Linear relationships among the seven components

[0085]

[0086] 2.4.5 Precision test

[0087] Accurately pipette the same mixed reference solution under "2.4.4", determine according to the chromatographic conditions under "2.4.1", inject 6 times continuously, and record the peak area. The RSD values ​​of the peak areas of arbutin, gallic acid, bergenin, catechin, 4-O-galloylbergenin, 11-O-galloylbergenin and catechin gallate were calculated to be 0.31%, 0.08%, 0.38%, 0.50%, 0.16%, 0.13% and 0.18%, respectively, indicating that this method has good precision.

[0088] 2.4.6 Stability test

[0089] The same test solution was sampled and measured at 0, 4, 8, 12, 24 and 48 h, and the RSD values ​​of the peak areas of arbutin, gallic acid, bergenin, catechin, 4-O-galloylbergenin, 11-O-galloylbergenin and catechin gallate were calculated to be 1.18%, 3.00%, 0.25%, 2.08%, 3.68%, 0.47% and 3.17%, respectively, indicating that the test sample prepared by this method is stable within 48 h.

[0090] 2.4.7 Repeatability test

[0091] Take the same batch of medicinal materials and prepare the test solution according to the method under "2.4.2". Prepare 6 parallel solutions and determine the content according to the chromatographic conditions under "2.4.1". The RSD values ​​of arbutin, gallic acid, bergenin, catechin, 4-O-galloylbergenin, 11-O-galloylbergenin and catechin gallate are 2.85%, 1.43%, 0.59%, 1.93%, 1.55%, 1.56% and 2.54%, respectively. The results show that the method has good repeatability.

[0092] 2.4.8 Sample recovery experiment

[0093] Take the same batch of Dapanlongqi medicinal material powder (S1), accurately weigh 6 portions, each 0.05g, add reference substances with approximately the same content as arbutin, gallic acid, bergenin, catechin, 4-O-galloylbergenin, 11-O-galloylbergenin and catechin gallate in the sample, prepare the test solution according to "2.4.2", and inject and measure according to the chromatographic conditions under "2.4.1", record the peak area and calculate the average sample recovery rate. The results showed that the average recoveries of arbutin, gallic acid, bergenin, catechin, 4-O-galloylbergenin, 11-O-galloylbergenin and catechin gallate were 99.09%, 98.88%, 99.10%, 100.93%, 102.05%, 98.39% and 100.34%, respectively, and the RSD values ​​were 2.40%, 2.83%, 1.36%, 2.75%, 1.46%, 1.79% and 2.72%, respectively, indicating that the experimental method was good.

[0094] 2.4.9 Sample content determination

[0095] Accurately weigh 0.1 g of each sample of Dapanlongqi medicinal material, prepare the sample solution according to the test sample preparation method under "2.4.2", measure according to the chromatographic conditions under "2.4.1", record the peak area, and calculate the contents of arbutin, gallic acid, bergenin, catechin, 4-O-galloylbergenin, 11-O-galloylbergenin and catechin gallate by external standard method. The results are shown in Table 4.

[0096] Table 4 Determination results of the contents of seven components in Dapanlongqi medicinal materials (mg·g -1 ,n=3)

[0097]

[0098] Example 3 Optimization of chromatographic conditions

[0099] The present invention investigates the effects of extraction solvent (50%, 60%, 70%, 80%, 100% methanol), solvent volume (20mL, 30mL, 40mL, 50mL) and ultrasonic extraction time (15, 30, 45, 60min) on the extraction efficiency of the components to be tested, comprehensively considers the peak area and separation of the main chromatographic peaks, and finally determines to use 60% methanol as solvent, 30mL of solvent volume, and ultrasonic 30min for extraction. Full wavelength scanning (190-400nm) was performed by a diode array detector, and it was found that the chromatographic peaks of the chemical components in Dapanlongqi were similar in ultraviolet spectra, and the maximum absorption peak was about 275nm, so this study selected 275nm as the detection wavelength for the determination of the content of Dapanlongqi medicinal materials. The mobile phases were methanol-water, acetonitrile-water, methanol-0.1% formic acid water, acetonitrile-0.1% formic acid water, and acetonitrile-0.1% phosphoric acid water. The results showed that the gradient elution of acetonitrile-0.1% phosphoric acid water could achieve a better separation of the Dapanlongqi medicinal material samples. 18 Chromatographic column (250 mm × 4.6 mm, 5 μm) and ThermoScientific Syncronis aQ C 18 The chromatographic column (250×4.6mm, 5μm) was investigated and finally the Agilent 5TC-C 18 The chromatographic column (250mm×4.6mm, 5μm) has a good separation effect on the chromatographic peaks in the Dapanlongqi sample; and different column temperatures (25, 30℃) were investigated, and the results showed that the separation effect of the Dapanlongqi sample was better at 25℃.

Claims

1. A method for constructing an HPLC fingerprint of Dapanlongqi medicinal material, characterized in that: When the HPLC chromatographic conditions are as follows, the HPLC fingerprint of the Dapanlongqi medicinal material has 14 characteristic fingerprint chromatographic peaks, wherein peak 1 is arbutin, peak 2 is gallic acid, peak 3 is bergenin, peak 5 is catechin, peak 7 is 4-O-galloylbergenin, peak 9 is 11-O-galloylbergenin, and peak 12 is catechin gallate; HPLC chromatographic conditions are as follows: The chromatographic column is Agilent 5TC-C 18 Chromatographic column, specification: 250mm×4.6mm, 5μm; Mobile phase: acetonitrile as phase A, 0.1% phosphoric acid aqueous solution as phase B; Gradient elution program Flow rate: 1.0 mL min -1 ; Column temperature: 25°C; detection wavelength: 275nm; injection volume: 10μL.

2. Application of the construction method described in claim 1 in quality control and component analysis of Panax notoginseng.

3. Application of the construction method of claim 1 in quality control of Radix Glehniae Radix, characterized in that: The application comprises the following steps: (1) Crush the Radix Panax Notoginseng, add methanol solution, perform ultrasonic treatment, and filter with a microporous membrane to obtain a test solution; (2) Take the test sample solution obtained in step (1) and perform HPLC detection to obtain an HPLC chart of the test sample, wherein the chromatographic conditions are as follows: The chromatographic column is Agilent 5TC-C 18 Chromatographic column, specification: 250mm×4.6mm, 5μm; Mobile phase: acetonitrile as phase A, 0.1% phosphoric acid aqueous solution as phase B; Gradient elution program Flow rate: 1.0 mL min -1 ; Column temperature: 25°C; detection wavelength: 275nm; injection volume: 10μL (3) Compare the HPLC chromatogram of the test sample obtained in step (2) with the HPLC fingerprint of the Dapanlongqi medicinal material. The Dapanlongqi medicinal material with a similarity of more than 0.92 is a qualified product.

4. The use according to claim 3, characterized in that: After the pulverization in step (1), the product is sieved through a 60-mesh sieve, the methanol solution is selected from a methanol solution with a volume fraction of 60%, the ultrasonic time is 20-40 minutes, and the microporous filter membrane is selected from a 0.22 μm filter membrane or a 0.45 μm filter membrane.

5. A method for analyzing the components of Dapanlongqi medicinal material, characterized in that: The method comprises the following steps: (1) Preparation of mixed solutions of 7 reference substances: Take appropriate amount of arbutin, gallic acid, bergenin, catechin, 4-O-galloylbergenin, 11-O-galloylbergenin, and catechin gallate reference substances, accurately weigh them, and add 60% methanol aqueous solution to prepare mixed reference substance solutions with mass concentrations of 530, 150, 520, 300, 96, 110, and 103 μg / mL, respectively; (2) Preparation of standard curves for each reference substance in step (1): Accurately measure 0.1, 0.5, 1.0, 5.0, and 10.0 mL of the mixed reference solution respectively into a 10 mL volumetric flask, add 60% methanol to dilute to the scale, filter with a 0.45 μm microporous filter membrane, and perform HPLC detection. Take the mass concentration x, μg / mL of each reference substance as the abscissa and the peak area value y as the ordinate. By calculation, the linear equation is obtained, as shown in the following table: Linear relationships among the seven components (3) Preparation of the test solution: crush the Radix Panax Psoraleae Radix, add methanol solution, perform ultrasonic treatment, and filter with a microporous membrane to obtain the test solution; (4) taking the test solution obtained in step (3), detecting it by HPLC, and calculating the contents of arbutin, gallic acid, bergenin, catechin, 4-O-galloyl bergenin, 11-O-galloyl bergenin, and catechin gallate in the Radix Pseudostellariae according to the concentrations of arbutin, gallic acid, bergenin, catechin, 4-O-galloyl bergenin, 11-O-galloyl bergenin, and catechin gallate reference substances and their retention times in the fingerprint spectrum and the linear equation in step (2); The chromatographic conditions of the HPLC detection in steps (2) and (4) are as follows: The chromatographic column is Agilent 5TC-C 18 Chromatographic column, specification: 250mm×4.6mm, 5μm; Mobile phase: acetonitrile as phase A, 0.1% phosphoric acid aqueous solution as phase B; Gradient elution program Flow rate: 1.0 mL min -1 ; Column temperature: 25°C; detection wavelength: 275nm; injection volume: 10μL.

6. The method according to claim 5, characterized in that In step (3), after the pulverization, the product is sieved through a 60-mesh sieve; the methanol solution is selected from a methanol solution with a volume fraction of 60%; the ultrasonic time is 20-40 minutes; and the microporous filter membrane is selected from a 0.22 μm filter membrane or a 0.45 μm filter membrane.

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