Rapid test device for wastewater analysis

Abstract

A rapid test device (200) is disclosed that includes a multi-parameter chemical test micro-particle (205) chip configured for input of a sample. A plurality of paper layers of the micro-particle (205) chip are in fluid communication, including a sample absorption element (215), a filtration element (220) configured to filter the input sample, and a sample distribution element (225) configured to distribute the input sample received from the filtration element (220) to the remainder of the plurality of paper layers. One or more reaction elements (230) associated with the multi-parameter chemical test of the input sample have one or more colorimetric reagents in fluid communication with the sample distribution element (225). A colorimetric result display element (235) in fluid communication with the one or more reaction elements (230) is configured to display, for a respective chemical test of the multi-parameter chemical test, a colorimetric effect of the test performed on the input sample with the at least one reaction element (230).

Description

Background Technology Technical Field

[0002] This invention generally relates to testing equipment for monitoring environmental conditions, and more particularly to rapid testing equipment.

[0003] Description of related technologies

[0004] Today, greater efforts are being made to monitor environmental conditions in various agricultural and manufacturing operations. With the world's growing population, ensuring these operations do not adversely affect ecosystems is a crucial element for the health and safety of future generations.

[0005] However, collecting samples and transporting them to laboratories for analysis to monitor environmental conditions is a slow and expensive process. For example, routine monitoring typically relies on titrations, electrometry, turbidimetric measurements, turbidity measurements, or colorimetric schemes performed in the laboratory. Portable kits are less expensive to use, but involve careful preparation steps and thorough cleaning of instruments after each use. The inherent logistical challenges of agricultural and manufacturing operations further increase the cost and complexity of monitoring environmental conditions. Rapid testing is required across various sectors, particularly to verify compliance with emission standards set forth in environmental compliance rules and regulations. Summary of the Invention

[0006] Based on various implementation schemes, this paper provides rapid testing equipment and manufacturing methods that offer inexpensive testing of compounds in liquids in a fast and simple manner. Results can be determined quickly without the need for time-consuming laboratory analysis.

[0007] In one embodiment, the rapid testing device includes a micropaper-based analytical device (micropad) chip configured for multi-parameter chemical testing of an input sample. Multiple paper layers of the micropad chip are in fluid communication, including a sample absorption element, a filter element configured to filter the input sample, and a sample dispensing element configured to dispense the input sample received from the filter element onto the remaining paper layers. One or more reaction elements associated with the multi-parameter chemical testing of the input sample have one or more colorimetric reagents in fluid communication with the sample dispensing element. A colorimetric result display element in fluid communication with one or more reaction elements is configured to display the colorimetric result of the test performed on the input sample using at least one reaction element for the corresponding chemical test in the multi-parameter chemical testing. The advantage of this configuration is that it provides a rapid and accurate testing method without requiring days of analysis in a laboratory. The presence of multiple compounds and their various concentrations can be easily analyzed through images of the test results.

[0008] In one embodiment, the plurality of paper layers are coated with a hydrophobic material configured to provide one or more hydrophilic channels on the paper layers. The coating can be constructed depending on the type of input sample being tested and the paper used.

[0009] In one embodiment, a timer element is configured to indicate the estimated time period during which the analysis of the input sample will be completed. By using a timing channel and a visual indicator, a quick and easy indicator is provided to let the user know the test is complete, rather than requiring them to look at an actual timer. An image of the test results can be provided to an image processor for analysis. A color correction element is configured to display the input sample to determine whether the colorimetric results should be adjusted based on the turbidity of the input sample. The color correction element improves the accuracy of the colorimetric results when the sample includes contaminants, debris, etc., that may skew the shading of the displayed test results.

[0010] In one embodiment, the colorimetric result display element is configured to display color shades corresponding to the concentration of a specific chemical substance in the input sample. Various shades and their associated concentration levels can be stored in a table for rapid concentration determination without requiring, for example, more complex titer assessments.

[0011] In one embodiment, the microparticle chip is configured to test the concentration and pH of excess nutrients in water. One or more colorimetric reagents are tailored for each test in a multi-parameter chemical assay. The multi-parameter testing capability of the rapid testing device provides rapid results, testing multiple compounds and their concentrations in a substantially simultaneous manner, saving time and costs.

[0012] In one embodiment, the microparticle chip is configured to test the concentration of at least one or more of phosphorus and convertible forms of nitrogen. These specific compounds tend to be associated with excessive amounts in agricultural operations. Such testing is significant for providing operations that comply with environmental regulations.

[0013] In one embodiment, multiple reaction elements associated with a multi-parameter chemical test of an input sample are configured for a multi-step chemical reagent test, which includes a first reaction element and a second reaction element. The first reaction element has one or more colorimetric reagents corresponding to at least a single-step chemical reaction process and a two-step chemical reaction process, while the second reaction element has one or more colorimetric reagents corresponding to a second stage of the two-step chemical reaction. The ability to perform single-step, two-step, or even three-step tests with a single rapid testing device provides time savings as well as increased testing efficiency and flexibility.

[0014] In one embodiment, the microparticle chip is configured to test the concentration of chemicals in one or more wastewater sources associated with industrial wastewater, hydraulic fracturing, and municipal sewage. These wastewater contaminants are a major concern for environmental safety.

[0015] In one embodiment, a cover element is disposed on at least a portion of the microparticle chip, wherein the cover element includes one or more visual identifiers associated with a rapid testing device, the visual identifiers including areas configured to display colorimetric results display elements.

[0016] In one embodiment, a portion of the covering element includes user instructions and manufacturing information.

[0017] In one embodiment, one or more visual identifiers of the overlay element include a Quick Response (QR) code with a device identification code, and a mark configured for image processing of the contrast color result display element.

[0018] In one embodiment, one or more visual identifiers of the overlay element include a color reference for image processing of the contrast color result display element.

[0019] In one embodiment, the covering element is made of cardboard and includes a front and a back.

[0020] In one embodiment, the colorimetric result display element is configured to display the colorimetric result of a test performed on the input sample with at least one reactive element and the color reference for capture by a camera.

[0021] According to one embodiment, a method of manufacturing a rapid testing device for multi-parameter chemical testing of an input sample includes providing a microparticle chip having multiple paper layers configured with a hydrophobic material arranged to provide one or more hydrophilic channels. The multiple paper layers are configured for corresponding functions by providing a sample absorption element layer configured to receive an input sample, arranging a filter element layer in fluid communication with the sample absorption element, and dispensing the input sample received from the filter element to the remaining paper layers via a sample dispensing element layer. Multiple reaction element layers are provided, associated with multi-parameter chemical testing of the input sample, wherein one or more colorimetric reagents are in fluid communication with the filter element. A colorimetric result display element layer in fluid communication with the multiple reaction elements is provided to display colorimetric results of the input sample tested with one or more of the multiple reaction elements.

[0022] These and other features will become apparent from the following detailed description of illustrative embodiments of the invention, which is read in conjunction with the accompanying drawings. Attached Figure Description

[0023] The accompanying drawings are illustrative embodiments. They do not show all embodiments. Other embodiments may be used alternatively or as an alternative. Details that may be obvious or unnecessary may be omitted to save space or for more efficient illustration. Some embodiments may be implemented with additional components or steps and / or without all components or steps shown. When the same numbers appear in different drawings, they refer to the same or similar components or steps.

[0024] Figure 1 An overview of a rapid testing apparatus according to an illustrative embodiment is shown.

[0025] Figure 2 An illustrative embodiment is shown. Figure 1 The paper-based microparticles in the rapid testing device shown.

[0026] Figure 3 A layer of paper-based microparticles according to an illustrative embodiment is shown.

[0027] Figure 4 This image shows some layers of microparticles used in a single-step chemical reagent to determine the pH of an input sample, according to an illustrative embodiment.

[0028] Figure 5 Some layers of microparticles used in two-step and three-step chemical reagents, according to illustrative embodiments, are shown.

[0029] Figure 6 An example of a protector layer for a rapid testing device according to an illustrative embodiment is shown.

[0030] Figure 7 This is a flowchart illustrating a method for manufacturing a rapid testing device according to an illustrative embodiment. Detailed Implementation

[0031] Overview

[0032] In the following detailed description, numerous specific details are illustrated by example to provide a thorough understanding of the relevant teachings. However, it should be understood that the teachings of this invention can be practiced without these details. In other instances, well-known methods, procedures, components, and / or circuits have been described at a relatively high level without detail to avoid unnecessarily obscuring aspects of these teachings.

[0033] For example, some of the most productive agricultural chains that ensure sufficient food supply are also responsible for generating large amounts of waste with high concentrations of organic matter and nutrients. Nitrogen and phosphorus are two instances of agriculturally relevant organic matter that, in large quantities, cannot be fully absorbed by the soil and requires treatment before being discharged into rivers and lakes. Nitrogen and phosphorus appear in wastewater in different forms. Nitrogen can exist in wastewater as ammonia (in balance with ammonium), organic nitrogen, nitrates, and nitrates. Phosphorus can exist in wastewater as phosphates. Excessive nitrogen and phosphorus have harmful effects on surrounding water bodies and all wildlife that depends on them.

[0034] In one illustrative embodiment, this disclosure relates to a rapid testing apparatus and method for testing wastewater. The paper-based rapid testing apparatus is configured to measure the concentration of excess nutrients using a colorimetric reagent.

[0035] Now refer in detail to the examples shown in the accompanying drawings and discussed below.

[0036] Example Architecture

[0037] Figure 1 An overview of a rapid testing apparatus 100 according to an illustrative embodiment is shown. It should be understood that the rapid testing apparatus shown is provided for illustrative purposes, and the appended claims are not limited to the description shown and illustrated. For ease of understanding of this specification, the front and rear portions of the cover element of the rapid testing apparatus 100 are shown. The paper microparticles may be covered only by the front cover, or may be contained within both the front and rear covers.

[0038] The rapid testing device 100 includes a sample absorption element 105. In an exemplary embodiment, the sample absorption element 105 extends from the rapid testing device to allow at least a portion of the sample absorption element to be immersed in the input sample.

[0039] Sample absorption element 105 is a microparticle chip used in rapid testing device 100 (see...). Figure 2 This is part of the illustrative embodiment. In this example, the microparticle chip includes five paper substrates 110 in its construction. However, rapid testing devices are not limited to this construction and can use fewer or more layers. "Layer 5" of the paper substrates 110 displays the reaction output of the input sample with various colorimetric reagents, which indicate the presence and concentration levels of multiple compounds. A background color correction element 115 is provided on layer 5 to aid in the accuracy of the display. For example, if the input sample is turbid or has a large number of impurities, the colorimetric results may be skewed because the test results may appear darker. The background color correction element serves as an integrity check, and if discoloration is present based on the presence of a turbid sample, a correction action can be taken by an image processing system analyzing the image from the testing device to determine the results.

[0040] In a non-limiting embodiment, timing channel 120 changes color to indicate the optimal or recommended time after the sample input absorption element 105 is immersed in the sample. Images of the preceding steps of the rapid testing device can be captured by a smartphone or other device. The smartphone or other device can relay the image information to, for example, a server to determine the concentrations of several compounds (e.g., in a multi-parameter test) and provide this information to the appropriate designated person. Alternatively, the smartphone may have an application that determines the test results in situ, for example, by using a table of chemicals and concentrations with colors and shading based on the output of the rapid testing device 100, utilizing a polynomial equation describing the relationship between color channels and concentrations, or by using machine learning algorithms (e.g., logistic regression, K-nearest neighbors, etc.) to estimate concentrations using color channels as information input.

[0041] Continue to refer to Figure 1 The rapid testing equipment may include identification information 125, such as a barcode or a Quick Response (QR) code. The type of test, location, manufacturer, and area being tested are some of the information that can be listed in the QR code 125. For example, in cases where images of the testing equipment captured by a camera may vary due to variations in camera capabilities, ambient lighting conditions, etc., which could otherwise introduce inaccuracies into the test, a color reference 135 can be provided for image processing. An image processing system (not shown) can correct the captured color readings based on a comparison with the color reference 135.

[0042] The rapid testing device may include, for example, user instructions 140 on the back or rear cover of the rapid testing device. It should be understood that the rapid testing device according to the invention is not limited to including, for example, user instructions 140. Figure 1 The explanation shown.

[0043] Figure 2 An illustrative embodiment is shown. Figure 1 The paper-based microparticles 205 of the rapid testing apparatus are shown. During operation of the rapid testing apparatus, the microparticles 205 typically have layers folded / stacked together. However, for ease of description, the microparticle chip layer 210 is also shown in a non-stacked / unfolded position.

[0044] The first layer is a sample absorption element 215, which serves as an impregnation input, for example, to load the input sample into a rapid testing device for analysis. The second layer is a filter element 220 in fluid communication with the sample absorption element 215. The filter element 220 is used to remove untested impurities, as well as impurities that may interfere with the passage of the input sample through microparticles.

[0045] The third layer is the sample dispensing element 225. The sample dispensing element 225 dispenses the input sample received from the filter element into the remaining paper layers among the multiple paper layers.

[0046] The fourth layer is the reaction element layer 230, which is configured for multi-parameter chemical testing of the input sample. The reaction layer 230 includes one or more colorimetric reagents in fluid communication with the sample dispensing element 225. It should be understood that multiple reaction element layers may be provided in the microparticles 205, and in such a configuration, the multiple reaction element layers can be referred to using common terms (e.g., first, second, etc.).

[0047] The fifth layer is a colorimetric result display element 235 that is in fluid communication with the reaction element layer 230. The result display element 235 is configured to display the colorimetric results of the input sample tested by the reaction element layer 230 for the corresponding chemical test in a multi-parameter chemical assay.

[0048] Figure 3 A layer 300 of paper-based microparticles according to an illustrative embodiment is shown. Figure 2 similar, Figure 3 The diagram shows a sample absorption element 315 that can be immersed in the input sample. A filter element 320 is in fluid communication with the sample absorption element 315 and is used to filter the input sample. Note that the filter element 320 in this illustrative embodiment includes a timing channel 321. A timing indicator 322 indicates that the test is complete. The sample dispensing element 325, the reaction element 330, and the result display element 335, which have channels 327 indicated by arrows, are also similar. Figure 2 The microparticles shown are also displayed by the timing element 322, which allows the tester to know that the analysis is complete.

[0049] Figure 4 Some layers of microparticles for a single-step chemical reagent used to determine the pH of an input sample are shown according to an illustrative embodiment. Timing element 422 is shaded to indicate test completion. Reaction element 430 shows various test shades performed on the input sample. For example, the pH process range using a universal reagent indicator in ethanol can produce various displays depending on the pH. For example, methyl yellow, methyl red, bromothymol blue, thymol blue, and phenolphthalein can be used to determine a rough pH range. Bromothymol blue can indicate a pH range of 6 to 7.6. Furthermore, a low pH range can be indicated by bromocresol green in ethanol. There may be little or no activity of a second reaction element indicated by display element 435.

[0050] Figure 5 Layers of microparticles used in two-step and three-step chemical reagents according to illustrative embodiments are shown. Reaction element 530 shows the two-step reagent, and display element 535 shows the two-step and three-step reagents, as indicated by the corresponding numbers. Figure 5 In this study, a two-step reagent can be used to detect phosphate, nitrite, and ammonia, while a three-step reagent can be used to detect nitrate levels.

[0051] Regarding phosphate detection, in one embodiment, the reagents include: Reagent 1 is a mixture of ammonium heptamolybdate tetrahydrate and potassium antimony(III) tartrate hydrate in sulfuric acid; Reagent 2 is ascorbic acid.

[0052] Regarding the detection of nitrite, in one embodiment, the reagents include: Reagent 1 is N-(1-naphthyl)ethylenediamine; Reagent 2 is sulfonamide.

[0053] Regarding the nitrate detection (three steps), in one embodiment, reagent 1 is a zinc suspension, reagent 2 is N-(1-naphthyl)ethylenediamine, and reagent 3 is sulfonamide.

[0054] Regarding ammonia detection, in one embodiment, reagent 1 is potassium hydrogen phthalate (KHPth), and reagent 2 is bromocresol purple. The colorimetric results of the two-step and three-step reagents are shown in 535.

[0055] Figure 6 An example of a protector layer 600 of a rapid testing apparatus according to an illustrative embodiment is shown. Protector layer 605 is a retained transparent cover, while protector layer 615 is to be peeled off before testing the input sample. It should be understood that the protector layer of the rapid testing apparatus is not limited to... Figure 6 The example shown above.

[0056] Example process

[0057] Having seen the foregoing overview of the example architecture, it may now be helpful to consider a more advanced discussion of the example process. To this end, Figure 7 yes Figure 1 , 2 Flowchart 700 shows the manufacturing process of the rapid testing equipment illustrated in Figure 3. More specifically, Figure 7 This is a flowchart illustrating a method for manufacturing a rapid testing device according to an illustrative embodiment.

[0058] In operation 710, multiple paper layers with a hydrophobic material are provided to the microparticles, the hydrophobic material being configured to provide hydrophilic channels. The microparticles are constructed to contain multiple reaction element layers having various colorimetric reagents embedded in the fibers of the paper channels and in fluid communication with sample dispensing elements. Constructing channels on the paper substrate allows for rapid, multi-parameter testing of multiple compounds from a single testing device.

[0059] At operation 720, the sample absorption element layer is configured to receive the input sample. For example... Figure 1 As shown, the sample absorption element layer can be extended from the rapid testing equipment to facilitate immersion in the input sample.

[0060] In operation 730, the filter element is arranged in fluid communication with the sample absorption element. Although in Figure 2 and 3In the diagram, the filter element is shown as having a general V-shape, but rapid testing equipment is not limited to this. The filter element removes impurities from the sample that are not being tested but could obstruct the flow of the input sample through the microparticle layer.

[0061] In operation 740, a sample dispensing element layer is provided to distribute the filtered input sample to the remaining paper layers of multiple paper layers for testing. Figure 3 The sample dispensing element layer 325 is shown, with channel 327 at the center of layer 3, but this structure is provided for illustrative purposes and it can also be arranged in another part of the microparticles.

[0062] In operation 750, multiple reaction element layers are provided that are associated with multi-parameter chemical testing of the input sample. The reaction element layers receive the input sample and colorimetric reagents to indicate the presence and concentration of various chemicals in the input sample.

[0063] In operation 760, the colorimetric result display element is arranged in fluid communication with multiple reaction elements to display the colorimetric results of the test on the input sample. Figure 4 and 5 The related descriptions discuss single-step, two-step, and three-step reagent tests that can be performed using rapid testing equipment.

[0064] Although the basic manufacturing method ends at operation 760, additional operations may be performed, such as including timer elements in the paper base layer, arranging microparticles in the cover element, and marking the cover element with identification information such as QR codes and color reference information.

[0065] in conclusion

[0066] Various embodiments of this teaching have been described for illustrative purposes, but are not intended to be exhaustive or limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles of the embodiments, their practical application, or improvements to existing technologies in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

[0067] While the content and / or other instances considered to be in the best possible state have been described above, it should be understood that various modifications may be made therein, and the subject matter disclosed herein can be implemented in various forms and instances, and the teachings can be applied to many applications, of which only a few have been described herein. The appended claims are intended to claim protection for any and all applications, modifications, and variations that fall within the true scope of this teaching.

[0068] The components, steps, features, purposes, benefits, and advantages discussed herein are merely illustrative. None of them, or the discussion relating to them, is intended to limit the scope of protection. While various advantages have been discussed herein, it will be understood that not all embodiments are necessarily intended to include all advantages. Unless otherwise stated, all measurements, values, ratings, positions, sizes, dimensions, and other specifications set forth in this specification, including those in the appended claims, are approximate and not precise. They are intended to have a reasonable scope consistent with the functionality associated with them and with the conventions of the art to which they pertain.

[0069] Many other embodiments have also been considered. These embodiments include those with fewer, additional, and / or different components, steps, features, purposes, benefits, and advantages. These also include embodiments in which components and / or steps are arranged and / or ordered in different ways.

[0070] While the foregoing has been described in conjunction with exemplary embodiments, it should be understood that the term "exemplary" means only as an example, and not the best or optimal. Apart from what has just been stated above, whether or not it is stated in the claims, nothing stated or described is intended or should be construed as causing any component, step, feature, object, benefit, advantage, or equivalent to be made public.

[0071] It should be understood that, unless otherwise specified herein, the terms and expressions used herein have the general meaning consistent with those in the respective fields of investigation and research to which they pertain. Relational terms such as "first" and "second" may be used merely to distinguish one entity or action from another, without necessarily requiring or implying any actual such relationship or order between these entities or actions. The terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but may also include other elements not expressly listed or inherent to such a process, method, article, or apparatus. Without further constraints, an element preceded by "a" or "an" does not exclude the presence of additional identical elements in the process, method, article, or apparatus that includes that element.

[0072] This summary of disclosure is provided to allow the reader to quickly determine the nature of this technical disclosure. It should be understood that it is not intended to interpret or limit the scope or meaning of the claims. Furthermore, as can be seen from the foregoing detailed description, various features have been grouped together in various embodiments to make this disclosure fluent. This method of disclosure should not be construed as reflecting an intention that the claimed embodiments have more features than those expressly recited in each claim. Rather, as reflected in the appended claims, the inventive subject matter lies in fewer than all features of a single disclosed embodiment. Therefore, the following claims are thereby incorporated into the detailed description, wherein each claim is independently claimed as a separate subject matter.

Claims

1. A rapid testing device, comprising: A microparticle chip configured for multi-parameter chemical testing of input samples, the microparticle chip having multiple fluidly connected paper layers, the multiple paper layers comprising: The sample absorption element is configured to receive the input sample; A filter element that is in fluid communication with the sample absorption element and is configured to filter the input sample; A sample dispensing element is configured to dispense an input sample received from the filter element into the remaining paper layers of the plurality of paper layers; Multiple reaction elements associated with multi-parameter chemical testing of the input sample, including multiple colorimetric reagents in fluid communication with a sample dispensing element; and A colorimetric result display element, which is in fluid communication with at least one of the plurality of reaction elements, and is configured to display the colorimetric results of the test performed on the input sample by the at least one reaction element for a corresponding chemical test in the multiparameter chemical test. The multiple reaction elements associated with the multi-parameter chemical testing of the input sample are configured for multi-step chemical reagent testing, including: A first reaction element having one or more colorimetric reagents corresponding to at least a single-step and a two-step chemical reaction process; and The second reaction element has one or more colorimetric reagents corresponding to the second stage of the two-step chemical reaction process.

2. The rapid testing apparatus of claim 1, wherein the plurality of paper layers are coated with a hydrophobic material, the hydrophobic material being configured to provide one or more hydrophilic channels.

3. The rapid testing device according to claim 2 further includes: A timer element is configured to indicate the estimated time period for completing the analysis of the input sample, and A color correction element is configured to display the input sample to determine whether the colorimetric result should be adjusted based on the turbidity of the input sample.

4. The rapid testing device of claim 3, wherein the colorimetric result display element is configured to display a shadow corresponding to the concentration of a specific chemical in the input sample.

5. The rapid testing device according to claim 3, wherein: The microparticle chip is configured to test the concentration and pH of excess nutrients in water; as well as The colorimetric reagents are customized for each test in multi-parameter chemical assays.

6. The rapid testing device according to claim 5, wherein the microparticle chip is configured to test the concentration of at least one of phosphorus or convertible forms of nitrogen.

7. The rapid testing device of claim 3, wherein the microparticle chip is configured to test the concentration of chemicals in one or more wastewaters associated with industrial wastewater, hydraulic fracturing, or municipal sewage.

8. The rapid testing apparatus of claim 3, further comprising a cover element disposed on at least a portion of the microparticle chip, wherein the cover element includes one or more visual identifiers associated with the rapid testing apparatus, the visual identifiers including areas configured to display the colorimetric result display element.

9. The rapid testing apparatus of claim 8, wherein a portion of the covering element includes user instructions and manufacturing information.

10. The rapid testing apparatus of claim 8, wherein the one or more visual identifiers of the overlay element comprise a quick-response QR code having a device identification code, and a mark configured for image processing of the colorimetric result display element.

11. The rapid testing apparatus of claim 8, wherein one or more visual identifiers of the overlay element include a color reference for image processing of the colorimetric result display element.

12. The rapid testing apparatus of claim 8, wherein the covering element comprises cardboard and includes a front portion and a rear portion.

13. The rapid testing apparatus of claim 11, wherein the colorimetric result display element is configured to display the colorimetric result of the test performed on the input sample with the at least one reactive element and the color reference for capture by a camera.

14. A method for manufacturing a rapid testing device for multi-parameter chemical testing of input samples, the method comprising: A microparticle chip having multiple paper layers is provided, the multiple paper layers being configured with a hydrophobic material, the hydrophobic material being arranged to provide one or more hydrophilic channels; Configure the multiple paper layers for their respective functions using the following steps: Provides a sample absorption element layer configured to receive input samples; A filter element layer is arranged in fluid communication with the sample absorption element; The input sample received from the filter element is distributed to the remaining paper layers of the plurality of paper layers via the sample distribution element layer; Provides a layer of multiple reaction elements associated with multi-parameter chemical testing of an input sample, wherein multiple colorimetric reagents are in fluid communication with the filter element; as well as The colorimetric results of a test performed on the input sample using one or more of the plurality of reactive elements are displayed by arranging a layer of colorimetric display elements in fluid communication with a plurality of reactive elements. The multiple reaction element layers provided for association with the multi-parameter chemical test also include configuration for multi-step chemical reagent testing via the following steps: Provides a first reaction element having one or more colorimetric reagents corresponding to at least a single-step and a two-step chemical reaction process; and A second reaction element is provided having one or more colorimetric reagents corresponding to the second stage of the two-step chemical reaction process.

15. The method of claim 14, further comprising indicating, by the timer element layer, the estimated time period for completing the analysis of the input sample.

16. The method of claim 15, further comprising providing a cap disposed on at least a portion of the microparticle chip, wherein the cap includes one or more visual identifiers associated with the rapid testing device, the visual identifiers including areas configured to display the colorimetric result display element.

17. A rapid testing device for measuring excess nutrients and pH in water, comprising: Multiple paper base layers, including: The sample absorption element layer is configured to receive the input sample; The filter element layer is configured to filter the input sample received from the sample absorption element; The sample dispensing element layer is configured to dispense the input sample received from the filter element into the remaining paper layers among a plurality of paper layers; Multiple reaction element layers are configured to perform multi-parameter chemical tests on the input sample received from the sample dispensing element. The multiple reaction elements include multiple colorimetric reagents to identify the concentration of at least one or more excess nutrients, including phosphorus or nitrogen in a convertible form. A colorimetric result display element layer, which is in fluid communication with at least one of the plurality of reaction elements, and is configured to display the colorimetric results of the test performed on the input sample using the at least one reaction element for a corresponding chemical test in the multiparameter chemical test; A timer element layer is configured to indicate that the test has been completed; and A color reference is configured to perform image processing on the displayed colorimetric results. When the plurality of reaction elements are configured for multi-step chemical reagent testing, they include: A first reaction element having one or more colorimetric reagents corresponding to at least a single-step and a two-step chemical reaction process; and The second reaction element has one or more colorimetric reagents corresponding to the second stage of the two-step chemical reaction process.

18. The rapid testing apparatus of claim 17, wherein the plurality of paper substrates are formed into microparticle chips, the microparticle chips are configured to perform multi-parameter testing and are coated with a hydrophobic material, the hydrophobic material being configured to provide one or more hydrophilic channels; and the rapid testing apparatus further comprises: A cover element is disposed on at least a portion of the microparticle chip, wherein the cover element includes one or more visual identifiers associated with the rapid testing device, the visual identifiers including areas configured to display the colorimetric result display element.

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