A glass culture dish steam sterilization apparatus for plasmid cloning culture

Abstract

The application discloses a glass culture dish steam sterilization equipment for plasmid clone culture, which is provided with a heat preservation middle layer, a heat preservation outer shell and a heat preservation inner layer, and according to the multi-layer heat preservation, the temperature is prevented from spreading too fast outward during the sterilization of the culture dish; meanwhile, during the sterilization of the culture dish, the pressure changing fan blade and the reversing fan blade are synchronously rotated, the airflow in the heat exchange cavity is blown out by the rotation of the pressure changing fan blade, the vacuum negative pressure is further used for heat preservation, the steam in the heat preservation cavity and the inner cavity of the heat preservation inner layer is accelerated to flow during the sterilization of the reversing fan blade, and the sterilization efficiency is improved; when the driving motor reversely rotates the pressure changing fan blade and the reversing fan blade, the airflow blown into the heat exchange cavity by the pressure changing fan blade accelerates the heat dissipation of the sterilization agent in the heat preservation cavity, and when the airflow in the heat preservation inner layer is driven to flow by the reversing fan blade, the temperature is gradually reduced, the water on the culture dish is dried, and the heat insulation steam sterilization and the cooling and drying effects are realized.

Description

Technical Field

[0001] This application relates to the technical field of sterilization equipment, and in particular to a steam sterilization device for glass culture dishes used in plasmid cloning culture. Background Technology

[0002] In medical plasmid cloning and culture, glass culture dishes are mostly used for bacterial preparation. Therefore, whether the glass culture dishes are thoroughly sterilized has a significant impact on the accuracy of the culture experiment.

[0003] Current disinfection methods mostly employ steam sterilization. However, after steam sterilization, water often remains on the surface of glass petri dishes, affecting the sterilization effect and the quality of bacterial culture. Currently, the main method for dealing with this water is to remove the sterilized dishes from the high-temperature steam and place them in a drying device to dry the surface. However, removing the sterilized dishes after high-temperature steam sterilization not only poses a risk of burns to the operator from overflowing steam, but also exposes the dishes to external air contamination, reducing their cleanliness. Furthermore, existing methods often use clamps to hold the petri dishes in place, creating blind spots in the sterilization process and resulting in incomplete high-temperature steam sterilization, ultimately impacting the sterilization effect. Summary of the Invention

[0004] This application proposes a steam sterilization device for glass culture dishes used in plasmid cloning culture, which has the advantages of heat-insulated steam sterilization and cooling air drying, in order to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this application adopts the following technical solution: A steam sterilization device for glass culture dishes used in plasmid cloning culture, comprising an insulating middle layer: the inner cavity of the insulating middle layer is provided with an upward-opening insulating chamber, and an insulating cover is provided on the surface of the insulating middle layer; the bottom of the inner wall of the insulating middle layer has an insulating inner layer fixedly installed in the insulating chamber by a cylindrical stake; a heating ring is fixedly installed at the bottom of the inner cavity of the insulating inner layer; a rotating shaft of a drive motor is movably installed in the middle of the surface of the insulating cover, and the rotating shaft of the drive motor is located in the inner cavity of the insulating inner layer; a reversing fan blade located at the top of the insulating inner layer is fixedly installed on the side wall of the rotating shaft of the drive motor; a placement base is fixedly installed at the bottom of the rotating shaft of the drive motor, and the outer wall of the placement base is slidably connected to the inner wall of the insulating inner layer; a culture dish is provided on the surface of the placement base; and an appropriate amount of disinfectant is placed in the insulating chamber.

[0006] Furthermore, the placement base is a perforated plate.

[0007] Furthermore, an anti-detachment inner locking block is fixedly installed on the surface of the placement base, a pressure plate is movably installed on the side wall of the rotating shaft of the drive motor, an adjusting nut is threadedly connected to the rotating shaft of the drive motor, and a gap is left between the pressure plate and the inner wall of the insulation inner layer.

[0008] Furthermore, a drying one-way air valve is fixedly installed on the surface of the pressure plate, and there are six drying one-way air valves installed at equal angles on the surface of the pressure plate.

[0009] Furthermore, an insulation shell is provided on the outer side of the insulation middle layer, and an upward-opening heat exchange chamber is provided inside the insulation shell. The bottom of the insulation middle layer and the bottom of the inner cavity of the insulation shell are fixedly connected by a pile. A box cover is fixedly installed on the surface of the insulation shell, and a hydraulic rod is fixedly installed on the side wall of the insulation shell. The top of the hydraulic rod is fixedly installed on the box cover. The drive motor is fixedly installed in the middle of the box cover. A venting groove is opened in the middle of the surface of the box cover. A pressure-changing fan blade is fixedly installed on the rotating shaft of the drive motor. A cooling one-way valve is fixedly installed at the bottom of the insulation shell.

[0010] Furthermore, heat dissipation fins are provided on the outer side of the insulation middle layer.

[0011] Furthermore, an actuating frame is movably installed on the inner wall of the insulation inner layer, and the actuating frame is U-shaped. A push rod is fixedly installed at one end of the actuating frame, and one end of the push rod passes through the insulation inner layer and the insulation middle layer and is located in the heat exchange cavity. A sensing push block is fixedly installed at one end of the push rod, and the sensing push block is located on the side wall of the insulation middle layer and is movably connected to the insulation middle layer. A return spring is fixedly installed at one end of the sensing push block. A support base is fixedly installed at the end of the actuating frame, and a roller is movably sleeved on the inner wall of the support base.

[0012] Furthermore, the roller is a cylindrical bar, and the roller is installed at an angle.

[0013] Furthermore, the outer side of the anti-detachment inner card block is designed to fit the inner wall of the culture dish. There are four sets of anti-detachment inner card blocks, and each set of anti-detachment inner card blocks is divided into four arc-shaped blocks. The inner wall of the culture dish is fixed by the four arc-shaped blocks.

[0014] This application provides a steam sterilization device for glass culture dishes used in plasmid cloning culture. By incorporating a middle insulating layer, an outer insulating layer, and an inner insulating layer, the device ensures that the temperature does not spread too rapidly during sterilization. Simultaneously, during sterilization, pressure-changing and reversing fan blades rotate synchronously. The pressure-changing fan blades blow air out of the heat exchange chamber, further maintaining the temperature through the extracted negative pressure vacuum. The reversing fan blades accelerate the steam flow within the insulating chamber and the inner insulating layer, enhancing sterilization efficiency. When the drive motor reverses the rotation of the pressure-changing and reversing fan blades, the airflow blown into the heat exchange chamber by the pressure-changing fan blades accelerates the dissipation of the disinfectant in the insulating chamber. Furthermore, the airflow within the inner insulating layer, driven by the reversing fan blades, gradually lowers the temperature and dries any water accumulated on the culture dish. Ultimately, this achieves both insulated steam sterilization and cooling / drying. Attached Figure Description

[0015] The accompanying drawings, which form part of this specification, illustrate embodiments disclosed in this application and, together with the specification, serve to explain the principles disclosed in this application.

[0016] This disclosure will become clearer with reference to the accompanying drawings and the following detailed description, wherein:

[0017] Figure 1 This is an overall appearance drawing;

[0018] Figure 2 This is a three-dimensional view of the overall interior.

[0019] Figure 3 This is an overall front sectional view;

[0020] Figure 4 for Figure 3 Sectional view at point AA;

[0021] Figure 5 Diagram of components on the box lid;

[0022] Figure 6 A three-dimensional view of the petri dish being secured with a pressure plate;

[0023] Figure 7 Diagram of the anti-detachment inner locking block;

[0024] Figure 8 This is a structural diagram of the components on the push rod;

[0025] Figure 9 This is a diagram of the outer shape of the insulation middle layer.

[0026] In the diagram: 1. Insulated outer shell; 100. Heat exchange chamber; 2. Box cover; 200. Ventilation groove; 3. Drive motor; 4. Hydraulic rod; 5. Pressure-reducing fan blade; 6. Insulated cover; 7. Petri dish; 8. Adjusting nut; 9. One-way air valve for drying; 10. Pressure plate; 11. Push rod; 110. Actuating frame; 111. Support base; 112. Roller; 12. Placement base; 120. Anti-detachment inner locking block; 13. Return spring; 14. Sensing push block; 15. Inner insulation layer; 16. Cooling one-way valve; 17. Heating ring; 18. Middle insulation layer; 180. Insulated chamber; 181. Heat dissipation fin; 19. Reversing fan blade. Detailed Implementation

[0027] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0028] Example 1

[0029] Please see Figure 2 and Figure 3The inner cavity of the insulation middle layer 18 is provided with an upward-opening insulation cavity 180, and an insulation cover 6 is provided on the surface of the insulation middle layer 18 to seal the insulation cavity 180. Then, an inner insulation layer 15 is fixedly installed in the insulation cavity 180 by a cylindrical pile at the bottom of the inner wall of the insulation middle layer 18. The inner insulation layer 15 is a cylindrical body that runs vertically through the insulation cavity 180, so that the inner cavity of the inner insulation layer 15 communicates with the insulation cavity 180. A heating ring 17 for heating is fixedly installed at the bottom of the inner cavity of the inner insulation layer 15. At the same time, the rotating shaft of the drive motor 3 is movably installed in the middle of the surface of the insulation cover 6, and the rotating shaft of the drive motor 3 is located in the inner cavity of the inner insulation layer 15. A reversing fan blade 19 located at the top of the inner insulation layer 15 is fixedly installed on the side wall of the rotating shaft of the drive motor 3. Meanwhile, a placement base 12 located in the inner cavity of the insulation inner layer 15 is fixedly installed at the bottom of the rotating shaft of the drive motor 3, and the outer wall of the placement base 12 is slidably connected to the inner wall of the insulation inner layer 15. A petri dish 7 is placed on the surface of the placement base 12, and an appropriate amount of disinfectant is placed in the insulation cavity 180. The amount of disinfectant added is sufficient to exceed the heating ring 17, thereby ensuring that during the actual disinfection process, the disinfectant in the inner cavity of the insulation inner layer 15 is heated by the heating ring 17, so that the disinfectant vapor rises and acts on the petri dish 7. At this time, in order to prevent the placement base 12 from blocking the upward movement of the vapor, the placement base 12 is set as a perforated plate to ensure the normal delivery of airflow. During the synchronous rotation of the reversing fan blade 19, it will be driven by the drive motor 3. When rotating in the forward direction, the airflow inside the inner insulation layer 15 is directed towards the bottom of the insulation cavity 180 until the airflow pressure at the bottom of the insulation cavity 180 increases, causing the disinfectant at the bottom of the insulation cavity 180 to be squeezed into the bottom of the inner insulation layer 15. As the airflow flows from the bottom of the insulation cavity 180 into the inner insulation layer 15, the airflow will pass through the disinfectant at the bottom of the inner insulation layer 15. The airflow then passes through the heating ring 17 for further heating and disinfection, thus blowing the airflow back onto the culture dish 7. This ensures that the culture dish 7 is sterilized. Because the inner insulation layer 15 is a vertical cylinder, the disinfectant vapor added by the heating ring 17 always impacts upwards, ensuring the intensity of heating of the culture dish 7, while also allowing for... The inner insulation layer 15 is placed in the insulation cavity 180, thus preventing the temperature inside the inner insulation layer 15 from diffusing too quickly to the outside. When the drive motor 3 drives the reversing fan blade 19 to rotate in the opposite direction, the reversing fan blade 19 will blow the airflow from the bottom of the inner insulation layer 15 into the insulation cavity 180, thereby blowing the disinfectant inside the inner insulation layer 15 into the insulation cavity 180. At this time, the heating ring 17 stops working, and with the disinfectant in the insulation cavity 180, the insulation cavity 180 is easier to cool from the outside. As the reversing fan blade 19 continuously blows airflow from the insulation cavity 180 into the inner insulation layer 15, the base 12 rotates the petri dish 7, causing the water accumulated on the petri dish 7 to be thrown out.On the other hand, the drying process is achieved through continuous air blowing by the reversing fan blades 19. The temperature drop is slowed by the continuous decrease in the temperature of the disinfectant at the bottom of the insulation chamber 180, preventing the petri dish 7 from cracking due to excessive temperature differences from the airflow. Simultaneously, during both sterilization and drying, the petri dish 7 remains within the insulation chamber 180 and is not exposed to the outside environment, thus preventing contamination from external air after steam sterilization.

[0030] in accordance with Figure 3 , Figure 6 and Figure 7 To improve the fixation strength of the culture dish 7, an anti-detachment inner clamping block 120 is fixedly installed on the surface of the base 12 to secure the culture dish 7. A pressure plate 10 is movably installed on the side wall of the drive motor 3's rotating shaft, positioned above the culture dish 7. An adjusting nut 8 is threadedly connected to the drive motor 3's rotating shaft to limit the position of the pressure plate 10. A gap is left between the pressure plate 10 and the inner wall of the insulation inner layer 15, preferably four to eight centimeters. During actual use, the position can be adjusted by adjusting the nut. The pressing plate 10 of plate 8 is placed above the petri dish 7. It not only ensures that the petri dish 7 will not detach from the anti-detachment inner clamp 120, but also blocks the airflow in the inner cavity of the heat preservation inner layer 15. Even when the reversing fan blade 19 rotates in the forward direction to steam sterilize the petri dish 7, the airflow below the heat preservation inner layer 15 will not flow away quickly through the gap between the pressing plate 10 and the middle heat preservation layer 18, thus causing the steam to accumulate below the pressing plate 10. This ensures that the petri dish 7 has enough steam for sterilization.

[0031] To prevent the airflow from being blocked by the pressure plate 10 when the reversing fan blades 19 are drying the culture dish 7, thus reducing the drying efficiency, a one-way air valve 9 for airflow from the upper to the lower part of the insulation inner layer 15 is fixedly installed on the surface of the pressure plate 10. There are six one-way air valves 9 installed at equal angles on the surface of the pressure plate 10. This ensures that during actual use, the airflow in the insulation inner layer 15 can flow quickly from top to bottom during air cooling, ensuring that the airflow flows quickly from the culture dish 7 during the drying process and enhancing the drying efficiency.

[0032] Example 2

[0033] Based on Example 1, please refer to Figure 2 , Figure 3 and Figure 5An insulation shell 1 is provided on the outside of the insulation middle layer 18, and an upward-opening heat exchange cavity 100 is provided inside the insulation shell 1. The insulation middle layer 18 is located in the heat exchange cavity 100, and the bottom of the insulation middle layer 18 is fixedly connected to the bottom of the inner cavity of the insulation shell 1 by means of a pile. A box cover 2 is fixedly installed on the surface of the insulation shell 1, and a hydraulic rod 4 is fixedly installed on the side wall of the insulation shell 1, with the top of the hydraulic rod 4 fixedly installed to the box cover 2. Thus, the box cover 2 is closed on the insulation shell 1 by pushing the box cover 2 up and down through the hydraulic rod 4. The drive motor 3 is fixedly installed on the insulation shell 1. A ventilated groove 200 communicating with the heat exchange chamber 100 is provided in the middle of the cover 2. A pressure-reducing fan blade 5 is fixedly installed between the ventilated groove 200 and the heat exchange chamber 100 on the rotating shaft of the drive motor 3. A cooling one-way valve 16 is fixedly installed at the bottom of the insulation shell 1 to achieve unidirectional airflow from the heat exchange chamber 100 to the outside. Therefore, in actual use, when the cover 2 is pushed upward by the hydraulic rod 4, the drive motor 3 synchronously drives the pressure-reducing fan blade 5, the insulation cover 6, the reversing fan blade 19, and the placement base 12 upward, causing the placement base 12 to move. The top of the insulation shell 1 facilitates the installation and removal of the petri dish 7. When sterilization is required, the hydraulic rod 4 drives the lid 2 to move down, pressing the lid 2 onto the insulation shell 1 and then onto the insulation middle layer 18 via the insulation cover 6. Then, when the drive motor 3 drives the reversing fan blade 19 to rotate forward for sterilization, the pressure-reducing fan blade 5 will rotate synchronously with the drive motor 3. When the drive motor 3 rotates forward, the pressure-reducing fan blade 5 will synchronously output the airflow from the heat exchange chamber 100 outwards, preventing the cooling check valve 16 from supplying external airflow into the heat exchange chamber 100, thus preventing the heat exchange chamber 100 from being exposed to external airflow. The pressure-reducing fan blades 5 continuously draw in negative pressure, creating a vacuum. As the heat exchange chamber 100 reduces the heat dissipation medium in the insulation layer 18, the heat exchange chamber 100 further insulates the insulation layer 18. When the drive motor 3 reverses and the petri dish 7 is dried, the pressure-reducing fan blades 5 deliver external airflow to the heat exchange chamber 100 through the ventilation grooves 200. As the airflow in the heat exchange chamber 100 increases, it is discharged from the cooling check valve 16, thereby accelerating the airflow in the heat exchange chamber 100, which in turn accelerates the heat dissipation of the insulation layer 18, further accelerating the heat dissipation of the disinfectant in the insulation chamber 180.

[0034] refer to Figure 2 , Figure 3 and Figure 8By providing heat dissipation fins 181 on the outer side of the insulation middle layer 18 to accelerate heat dissipation, the airflow will flow through the heat dissipation fins 181 during the reverse rotation of the drive motor 3, thus accelerating the heat dissipation of the insulation middle layer 18. At the same time, when the drive motor 3 reverses, the disinfectant in the insulation inner layer 15 will be blown into the insulation cavity 180. Therefore, the disinfectant in the insulation cavity 180 will cool down faster when passing through the heat dissipation fins 181, thereby accelerating the rate of temperature drop of the petri dish 7 during air drying.

[0035] Example 3

[0036] Based on Example 2, please refer to Figure 3 , Figures 6-9A U-shaped actuating bracket 110 is movably installed on the inner wall of the inner insulation layer 15, above the surface of the base 12. A push rod 11 is fixedly installed at one end of the actuating bracket 110, and one end of the push rod 11 passes through the inner insulation layer 15 and the middle insulation layer 18 and is located in the heat exchange cavity 100. A sensing push block 14 is fixedly installed at one end of the push rod 11. The sensing push block 14 is cuboid in shape and is located on the side wall of the middle insulation layer 18 and movably connected to it. A [missing information - likely a device or component] is fixedly installed at one end of the sensing push block 14. A return spring 13 is located outside the push rod 11, and the return spring 13 is located in the inner wall of the insulation middle layer 18. At the same time, a support seat 111 located on one side of the pressure plate 10 is fixedly installed at the end of the actuating frame 110, and a roller 112 is movably sleeved on the inner wall of the support seat 111. The roller 112 is a cylindrical rod, and the roller 112 is installed at an angle, with the center line of the roller 112 and the surface of the actuating frame 110 at an angle of 30 to 75 degrees. This ensures that during actual use, when the petri dish 7 is sterilized, the airflow in the heat exchange chamber 100 will be drawn out, causing the heat exchange chamber 100 to... The pressure in the container decreases, causing the push rod 11 to move outward. This causes the agitator 110 to follow the push rod 11, and the inner wall of the agitator 110 contacts the side wall of the petri dish 7. During sterilization, the petri dish 7 rotates circumferentially with the placement base 12 due to its rotation. When the petri dish 7 approaches the inner wall of the agitator 110, it is agitated by the inner wall of the agitator 110, forcing the petri dish 7 to rotate around its center line. This prevents the petri dish 7 from being unable to undergo complete steam sterilization due to its fixed position. Meanwhile, the petri dish 7 is subjected to air... During the drying process, airflow is introduced into the heat exchange chamber 100, causing the pressure in the heat exchange chamber 100 to increase because not all of it can flow out through the cooling check valve 16. The increased pressure in the heat exchange chamber 100 pushes the sensing push block 14 towards the return spring 13, causing the roller 112 to press against the outer top of the pressure plate 10, thereby forcing the pressure plate 10 to have a downward pressing force. This ensures that the culture dish 7 is firmly fixed during the air drying process, and the air drying rate of the culture dish 7 can be increased by increasing the rotation speed of the drive motor 3.

[0037] By designing the outer side of the anti-detachment inner locking block 120 as an arc shape adapted to the inner wall of the petri dish 7, and by having four sets of anti-detachment inner locking blocks 120, each set containing four arc-shaped blocks, the inner wall of the petri dish 7 is fixed using these four arc-shaped blocks. By making the anti-detachment inner locking block 120 of rubber, a certain gap is maintained between the pressure plate 10 and the top of the petri dish 7 during sterilization, thanks to the adjustment nut 8. This ensures that the top of the petri dish 7 does not contact the bottom of the pressure plate 10 during sterilization, thus preventing incomplete sterilization due to continuous contact with the pressure plate 10. Furthermore, the petri dish 7 will not detach from the pressure plate 10 and the placement base 12 when rotated by the agitator 110. When the petri dish 7 is air-drying, the pressure plate 10 is pressed by the roller 112, thus enhancing the fixation strength of the petri dish 7 during air-drying.

[0038] Based on Embodiments 1 to 3, the working principle of this device is as follows: When an appropriate amount of disinfectant is placed in the insulation cavity 180 and the drive motor 3 rotates in the forward direction, the airflow at the bottom of the inner cavity of the inner insulation layer 15 is transported into the insulation cavity 180, thereby causing the disinfectant in the insulation cavity 180 to flow into the bottom of the inner cavity of the inner insulation layer 15. At the same time, the airflow in the heat exchange cavity 100 is drawn outward by the pressure-changing fan blades 5, so that a negative pressure vacuum is formed in the heat exchange cavity 100 for heat insulation. Meanwhile, when the pressure in the heat exchange cavity 100 decreases, the sensing push block 14 moves outward and pulls the push rod 11 to bring the agitator 110 close to the outside of the culture dish 7. The inner wall of the agitator 110 contacts the outside of the culture dish 7, realizing the rotation of the culture dish 7 and avoiding the phenomenon that the culture dish 7 cannot be fully disinfected due to being fixed.

[0039] During air drying, the drive motor 3 reverses, and the pressure-changing fan blades 5 blow external airflow into the heat exchange chamber 100 and output it from the cooling one-way valve 16. The pressure in the heat exchange chamber 100 increases, and the actuating frame 110 drives the support base 111 to approach the pressure plate 10. Under the pressure of the roller 112, the pressure plate 10 is pressed downward to ensure the fixation strength of the culture dish 7. At this time, if you want to enhance the air drying intensity, you can increase the rotation speed of the drive motor 3, which will increase the downward pressure of the pressure plate 10 and simultaneously enhance the fixation strength of the culture dish 7 as the air drying intensity increases.

[0040] Once the drying process is complete, the spring force of the return spring 13 will cause the actuating frame 110 to retract into the inner insulation layer 15, thus preventing the placement base 12 from being blocked by the actuating frame 110 when it is lifted outward by the hydraulic rod 4.

Claims

1. A steam sterilization device for glass culture dishes used in plasmid cloning culture, characterized in that, The insulation includes an inner insulation layer (18): the inner cavity of the inner insulation layer (18) is provided with an upward-opening insulation cavity (180), and the surface of the inner insulation layer (18) is provided with an insulation cover (6). The bottom of the inner wall of the inner insulation layer (18) has an inner insulation layer (15) fixedly installed in the insulation cavity (180) by a cylindrical pile. The bottom of the inner cavity of the inner insulation layer (15) is fixedly installed with a heating ring (17). The rotating shaft of the drive motor (3) is movably installed in the middle of the surface of the insulation cover (6). The rotating shaft of the drive motor (3) is located in the inner cavity of the heat insulation inner layer (15). The rotating shaft of the drive motor (3) is fixedly installed with a reversing fan blade (19) located at the top of the heat insulation inner layer (15). The bottom of the rotating shaft of the drive motor (3) is fixedly installed with a placement base (12), and the outer wall of the placement base (12) is slidably connected to the inner wall of the heat insulation inner layer (15). A petri dish (7) is provided on the surface of the placement base (12). An appropriate amount of disinfectant is placed in the heat insulation cavity (180). The surface of the placement base (12) is fixedly installed with an anti-detachment inner clamp (120), and a pressure plate (10) is movably installed on the side wall of the rotating shaft of the drive motor (3). The rotating shaft of the drive motor (3) is threadedly connected with an adjusting nut (8), and a gap is left between the pressure plate (10) and the inner wall of the heat insulation inner layer (15). The outer side of the insulation middle layer (18) is provided with an insulation shell (1), and the inside of the insulation shell (1) is provided with an upward-opening heat exchange chamber (100). The bottom of the insulation middle layer (18) and the bottom of the inner cavity of the insulation shell (1) are fixedly connected by a pile. A box cover (2) is fixedly installed on the surface of the insulation shell (1), and a hydraulic rod (4) is fixedly installed on the side wall of the insulation shell (1). The top of the hydraulic rod (4) is fixedly installed on the box cover (2). The drive motor (3) is fixedly installed in the middle of the box cover (2). A venting groove (200) is opened in the middle of the surface of the box cover (2). A pressure-changing fan blade (5) is fixedly installed on the rotating shaft of the drive motor (3). A cooling one-way valve (16) is fixedly installed at the bottom of the insulation shell (1). An actuating frame (110) is movably installed on the inner wall of the inner insulation layer (15), and the actuating frame (110) is U-shaped. A push rod (11) is fixedly installed at one end of the actuating frame (110), and one end of the push rod (11) passes through the inner insulation layer (15) and the middle insulation layer (18) and is located in the heat exchange chamber (100). A sensing push block (14) is fixedly installed at one end of the push rod (11), and the sensing push block (14) is located on the side wall of the middle insulation layer (18) and is movably connected to the middle insulation layer (18). A reset spring (13) is fixedly installed at one end of the sensing push block (14). A support seat (111) is fixedly installed at the end of the actuating frame (110), and a roller (112) is movably sleeved on the inner wall of the support seat (111).

2. The steam sterilization device for glass culture dishes for plasmid cloning culture according to claim 1, characterized in that, The placement base (12) is a perforated plate.

3. The steam sterilization device for glass culture dishes for plasmid cloning culture according to claim 1, characterized in that, The pressure plate (10) is fixedly installed with a drying one-way air valve (9), and there are six drying one-way air valves (9). The six drying one-way air valves (9) are installed at equal angles on the surface of the pressure plate (10).

4. The steam sterilization device for glass culture dishes for plasmid cloning culture according to claim 1, characterized in that, The outer side of the insulation middle layer (18) is provided with heat dissipation fins (181).

5. The steam sterilization device for glass culture dishes for plasmid cloning culture according to claim 1, characterized in that, The roller (112) is a cylindrical rod, and the roller (112) is installed at an angle.

6. The steam sterilization device for glass culture dishes for plasmid cloning culture according to claim 1, characterized in that, The outer side of the anti-detachment inner card block (120) is designed to fit the inner wall of the culture dish (7). There are four sets of the anti-detachment inner card block (120). Each set of the anti-detachment inner card block (120) is divided into four arc-shaped blocks, which are used to fix the inner wall of the culture dish (7).

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