Transistor microfluidic chip by finger pressure and its operating method

Abstract

The present application provides a finger-pressing transistor micro-channel chip, which comprises a body, a finger-pressing structure, a printed circuit board and a transistor chip. The body comprises a sample dropping port, a detection flow channel, a sample dropping port and an absorption groove. The sample dropping port is connected to the detection flow channel. The absorption groove comprises an absorption pad. The sample dropping groove of the finger-pressing structure is connected to the sample dropping port and an external space of the chip. The printed circuit board is fixedly arranged on the body. The transistor chip is electrically connected to the printed circuit board. Thus, the finger-pressing transistor micro-channel chip of the present application can avoid the closed cavity design of the existing finger-pressing micro-channel chip by connecting the sample dropping groove of the finger-pressing structure to the external space of the chip, thereby reducing the volume and saving the preparation cost. The present application also provides an operation method of the finger-pressing transistor micro-channel chip.

Description

Technical Field

[0001] This invention relates to a microfluidic chip (wafer) and its operating method, and particularly to a finger-pressed transistor microfluidic chip for detection using transistors and its operating method. Background Technology

[0002] In view of the development of biomedicine, how to quickly and accurately diagnose diseases in vitro has become the goal of related fields, and devices that require rapid detection have also flourished.

[0003] A biosensor is an integrated analytical system developed using the characteristics of biological reactions and specific identification. It consists of two parts: a biosensor and a signal transmission and conversion device. During detection, the analyte reacts with the biosensor and generates light, heat, mass, or electrochemical signals. The signal transmission and conversion device then converts these signals into output signals for subsequent analysis, thus achieving the detection purpose.

[0004] Furthermore, the market offers a diverse range of biosensors for detection, and the combination and use of different types of biometric identification devices and signal transmission conversion devices allow biosensors to meet various detection needs. Therefore, providing a biosensor that combines low cost and high detection efficiency, and further applying it to biomedical testing, has become a goal pursued by the academic and industrial communities. Summary of the Invention

[0005] One embodiment of the present invention provides a finger-pressable transistor microfluidic chip, comprising a body, a finger-pressable structure, a printed circuit board, and a transistor chip. The body includes a sample inlet, a detection channel, a sample inlet, and an absorption groove. The detection channel has a first end and a second end, wherein the first end is connected to the sample inlet. The sample inlet is connected to the detection channel. The absorption groove is connected to the second end of the detection channel, and includes an absorption pad. The finger-pressable structure is disposed on the body and has a sample inlet, wherein the sample inlet is connected to the sample inlet and to an external space of the chip, and the finger-pressable structure includes a first pressing piece and a second pressing piece. The first pressing piece includes a first cavity, wherein the first pressing piece is made of a cushioning material. The second pressing piece is disposed between the body and the first pressing piece, wherein the second pressing piece includes a second cavity, the second cavity correspondingly connected to the first cavity to form the sample inlet, and the second pressing piece is made of a rigid material. A printed circuit board is fixedly mounted on the body and includes a receiving slot. A transistor chip is embedded in the receiving slot and is electrically connected to the printed circuit board. The transistor chip includes multiple working electrodes, and the transistor chip corresponds to a detection channel, exposing the working electrodes in the detection channel.

[0006] According to the aforementioned embodiment of the finger-pressed transistor microchannel chip, the printed circuit board may be disposed on a surface of the body that is different from the finger-pressed structure.

[0007] According to the aforementioned embodiment of the finger-pressable transistor microchannel chip, the printed circuit board may further include a reference electrode exposed in the sample dropper.

[0008] According to the aforementioned embodiment of the finger-pressable transistor microchannel chip, the transistor chip may be an ion-sensitive field-effect transistor (ISFET) chip.

[0009] According to the aforementioned embodiment of the finger-pressable transistor microchannel chip, the body may further include two welding grooves, which are respectively disposed on both sides of the detection channel. The two welding grooves are used to weld multiple electrodes of the printed circuit board to the working electrodes of the transistor chip to form an electrical conduction circuit.

[0010] According to the aforementioned embodiment of the finger-pressing transistor microchannel chip, the material of the first pressing piece can be foam, silicone, rubber, or a combination thereof.

[0011] According to the aforementioned embodiment of the finger-pressable transistor microchannel chip, the sample drop port can be opened on the detection channel and located on the side of the detection channel near the second end.

[0012] According to the aforementioned embodiment of the finger-pressure transistor microchannel chip, the sample drop port and the sample outlet are disposed separately on the body, and the sample drop port can be connected to the detection channel through a connecting channel.

[0013] According to the aforementioned embodiment of the finger pressure transistor microchannel chip, there is an angle between the long axis of the detection channel and the connecting channel, and the size of the angle can be from 10° to 90°.

[0014] According to the aforementioned embodiment of the finger-pressable transistor microfluidic chip, the body may include a body surface, and the body may be sequentially composed of an upper substrate, a pipeline substrate, and a bottom plate from the body surface downwards. The upper substrate, pipeline substrate, bottom plate, and transistor chip are sequentially stacked to form a detection channel. The upper substrate may include a first substrate opening, the pipeline substrate may include a first pipeline opening, the first substrate opening is correspondingly connected to the first pipeline opening, and the first substrate opening, the first pipeline opening, and bottom plate are sequentially stacked to form an absorption groove.

[0015] According to the aforementioned embodiment of the finger-pressable transistor microchannel chip, the opening size of the first substrate opening can be smaller than the surface area of ​​the absorption pad.

[0016] According to the aforementioned embodiment of the finger-pressable transistor microchannel chip, the substrate, the printed circuit board and the transistor chip are bonded to each other, and the substrate includes a reference electrode opening, a chip electrode opening and two soldering groove openings.

[0017] Therefore, by connecting the sample-dropping groove of the finger-pressure structure to the external space of the chip, the finger-pressure transistor microfluidic chip of the present invention not only eliminates the closed cavity design of existing finger-pressure microfluidic chips, but also reduces the size of the finger-pressure transistor microfluidic chip and saves manufacturing costs. Furthermore, by using a cushioning material for the first pressing plate and a rigid material for the second pressing plate, the rigid second pressing plate not only limits the depth of the user's pressure on the first pressing plate, but also prevents the user's fingers from contacting the liquid in the sample-dropping opening during pressing, thus making the use of the finger-pressure transistor microfluidic chip of the present invention safer and more convenient. In addition, by providing an absorbent pad in the absorption groove, waste liquid discharged from the detection channel can be absorbed, avoiding waste liquid contamination or leakage, thereby improving the detection accuracy and ease of use of the finger-pressure transistor microfluidic chip of the present invention.

[0018] Another embodiment of the present invention provides a method for operating a finger-pressable transistor microfluidic chip, comprising the following steps: A finger-pressable transistor microfluidic chip as described above is provided, wherein the finger-pressable transistor microfluidic chip is electrically connected to a detector. A first measurement step is performed, wherein a first detection liquid is added to a sample dropper, so that the first detection liquid enters from the sample dropper and fills the detection channel. At this time, the detector is operated to power on the printed circuit board and drive the transistor chip to measure a background value of each working electrode. A first press-and-empty step is performed, wherein the finger-pressable structure is pressed to close the sample dropper, so that the first detection liquid is discharged from the detection channel and enters the absorption tank. A sample reaction step is performed, wherein a sample to be tested is added to a sample dropper, so that the sample to be tested fills the detection channel and reacts for a predetermined reaction time. A second press-and-empty step is performed, wherein the finger-pressable structure is pressed to close the sample dropper, so that the sample to be tested is discharged from the detection channel and enters the absorption tank. A second measurement step is performed, in which a second detection solution is added to the sample dropper, allowing the solution to enter through the sample dropper and fill the detection channel. At this time, the detector is operated to power on the printed circuit board and drive the transistor chip to measure a detection value at each working electrode. A data analysis step is then performed, analyzing the background and detection values ​​of each working electrode to obtain a detection result for each working electrode.

[0019] According to the operation method of the finger-pressure transistor microfluidic chip of the aforementioned embodiment, the amount of the first detection liquid added is A, and the amount of the second detection liquid added is B, which can satisfy the following condition: 2A≤B.

[0020] According to the operation method of the finger-pressed transistor microchannel chip of the aforementioned embodiment, the printed circuit board may be disposed on a surface of the body that is different from the finger-pressed structure.

[0021] According to the operation method of the finger-pressed transistor microfluidic chip of the aforementioned embodiment, the printed circuit board may further include a reference electrode exposed in the sample dropper.

[0022] The operation method of the finger-pressed transistor microchannel chip according to the aforementioned embodiments, wherein the transistor chip may be an ion-sensitive field-effect transistor chip.

[0023] According to the operation method of the finger-pressed transistor microchannel chip of the aforementioned embodiment, the material of the first pressing piece can be foam, silicone, rubber or a combination thereof.

[0024] According to the operation method of the finger-pressed transistor microfluidic chip of the aforementioned embodiment, the sample drop port can be opened on the detection channel and located on the side of the detection channel near the second end.

[0025] According to the operation method of the finger-pressure transistor microfluidic chip of the aforementioned embodiment, the sample drop port and the sample drop port are disposed separately on the body, and the sample drop port can be connected to the detection channel through a connecting channel.

[0026] According to the operation method of the finger pressure transistor microchannel chip of the aforementioned embodiment, there is an angle between the long axis of the detection channel and the connecting channel, and the size of the angle can be from 10° to 90°.

[0027] Therefore, the operation method of the finger-pressure transistor microfluidic chip of the present invention uses the finger-pressure transistor microfluidic chip of the present invention to test the sample. The liquid in the test channel can be emptied multiple times to achieve a cleaning effect, so that the finger-pressure transistor microfluidic chip of the present invention can perform multiple repeated measurements. This improves the detection accuracy and ease of use of the operation method of the finger-pressure transistor microfluidic chip of the present invention, and has application potential in related markets. Attached Figure Description

[0028] To make the above and other objects, features, advantages and embodiments of the present invention more apparent and understandable, the accompanying drawings are described below:

[0029] Figure 1 A schematic diagram of a finger-pressable transistor microchannel chip according to an embodiment of the present invention is shown.

[0030] Figure 2 Show Figure 1 An exploded view of a finger-pressed transistor microchannel chip;

[0031] Figure 3 Show Figure 1Another exploded view of the finger-pressed transistor microchannel chip;

[0032] Figure 4 A schematic diagram of a finger-pressable transistor microchannel chip according to another embodiment of the present invention is shown;

[0033] Figure 5 Show Figure 4 An exploded view of a finger-pressed transistor microchannel chip;

[0034] Figure 6 A flowchart illustrating the steps of an operation method for a finger-pressable transistor microchannel chip according to another embodiment of the present invention is shown.

[0035] Figure 7 A sequential operation diagram of a finger-pressable transistor microchannel chip according to a first embodiment of the present invention is shown; and

[0036] Figure 8 A sequential operation diagram of a finger-pressable transistor microchannel chip according to a second embodiment of the present invention is shown;

[0037] Figure 9 To present Figure 7 Color images;

[0038] Figure 10 To present Figure 8 Color illustrations.

[0039] Explanation of reference numerals in the attached figures:

[0040] 100, 200, 400, 500: Finger-pressable transistor microchannel chips

[0041] 110,210: Ontology

[0042] 1101,2101: Body surface

[0043] 1102: Upper substrate

[0044] 1103: Pipeline substrate

[0045] 1104: Base Plate

[0046] 1105: First substrate opening

[0047] 1106: First pipe opening

[0048] 1107: Reference electrode opening

[0049] 1108: Chip electrode opening

[0050] 1109: Welding groove opening

[0051] 111,211: Sample dispensing port

[0052] 112,212,412,512: Detection channels

[0053] 1121: First End

[0054] 1122,4122,5122: Second end

[0055] 113,213,413,513: Sample dropper

[0056] 114, 214, 414, 514: Absorption tanks

[0057] 115, 415, 515: Absorbent pads

[0058] 116: Welding groove

[0059] 120, 220, 420, 520: Finger pressure structure

[0060] 1201, 2201, 4201, 5201: Sample Dropping Cells

[0061] 121,221: First press tablet

[0062] 1211: First cavity

[0063] 122,222: Second pressing tablet

[0064] 1221: Second cavity

[0065] 130, 230: Printed Circuit Board

[0066] 131,231: Receiving slot

[0067] 132,232: Reference electrodes

[0068] 133: Electrode

[0069] 140, 240: Transistor chips

[0070] 141,241: Working electrode

[0071] 217,517: Connecting channels

[0072] 218: Stomata

[0073] 219: Sealing film

[0074] 300: Operation method of finger-pressable transistor microfluidic chip

[0075] 310, 320, 330, 340, 350, 360, 370: Steps

[0076] θ: included angle

[0077] A: The amount of the first test solution added

[0078] B: Amount of the second detection solution added Detailed Implementation

[0079] The various embodiments of the present invention will be discussed in more detail below. However, these embodiments can be applications of various inventive concepts and can be implemented in various different specific scopes. The specific embodiments are for illustrative purposes only and are not limited to the scope of disclosure.

[0080] I. The finger-pressable transistor microchannel chip of the present invention

[0081] Please refer to Figure 1 and Figure 2 , Figure 1 A schematic diagram of a finger-pressable transistor microchannel chip 100 according to an embodiment of the present invention is shown. Figure 2 Show Figure 1 An exploded view of the finger-pressed transistor microfluidic chip 100. The finger-pressed transistor microfluidic chip 100 includes a body 110, a finger-pressed structure 120, a printed circuit board 130, and a transistor chip 140.

[0082] The body 110 includes a sample drop port 111, a detection channel 112, a sample drop port 113, and an absorption tank 114.

[0083] The detection channel 112 has a first end 1121 and a second end 1122. The first end 1121 is connected to the sample dispensing port 111, and the second end 1122 is connected to the absorption tank 114. The sample dispensing port 113 is connected to the detection channel 112 via a conduit. Specifically, in the finger-pressure type transistor microfluidic chip 100 of the present invention, the detection channel 112 is a straight channel, and the sample dispensing port 113 is formed on the detection channel 112 and located on the side of the detection channel 112 near the second end 1122. By having the sample dispensing port 111 and the sample dispensing port 113 respectively located at the two ends of the detection channel 112, contamination of other important components such as the reference electrode 132 on the printed circuit board 130 by bioactive substances in the sample to be tested can be avoided, thus ensuring the detection accuracy and extending the service life of the finger-pressure type transistor microfluidic chip 100 of the present invention. Furthermore, since the sample drop port 113 is located on the detection channel 112, the sample drop port 113 acts as a liquid volume control valve. The detection liquid or the liquid of the sample to be tested in the detection channel 112 will stop at the sample drop port 113, thereby controlling the volume of the detection liquid or the sample to be tested filling the detection channel 112 and ensuring that the liquid covers the working electrode 141 of the transistor chip 140, thereby improving the detection accuracy of the finger pressure transistor microfluidic chip 100 of the present invention.

[0084] The absorption tank 114 includes an absorption pad 115. By providing the absorption pad 115 in the absorption tank 114, waste liquid discharged from the detection channel 112 can be absorbed, avoiding waste liquid contamination or leakage, thereby improving the detection accuracy and ease of use of the finger-pressure transistor microfluidic chip 100 of the present invention. In addition, the absorption pad 115 is made of highly absorbent materials such as paper, cotton, or super absorbent polymer materials, but the present invention is not limited to these.

[0085] A finger-pressure structure 120 is disposed on the body 110 and has a sample groove 1201. The sample groove 1201 is connected to the sample outlet 111 and to an external space of the chip (not shown). Specifically, the finger-pressure structure 120 allows the user to press it. When the user presses the finger-pressure structure 120 with their finger, the finger covers and seals the sample groove 1201, isolating it from the external space of the chip and forming a configuration similar to a closed cavity. Simultaneously, the user pressing the finger-pressure structure 120 causes a change in the volume of the sample groove 1201, correspondingly changing the internal pressure and creating a vacuum pump-like effect. This causes the detection liquid or sample to be tested in the detection channel 112 to be discharged, facilitating detection, cleaning, and repeated measurements.

[0086] Furthermore, the finger-pressing structure 120 includes a first pressing piece 121 and a second pressing piece 122. The first pressing piece 121 includes a first cavity 1211, wherein the material of the first pressing piece 121 is a cushioning material. Preferably, the material of the first pressing piece 121 can be foam, silicone, rubber, or other compressible materials, but the present invention is not limited thereto. The second pressing piece 122 is disposed between the body 110 and the first pressing piece 121, wherein the second pressing piece 122 includes a second cavity 1221, the second cavity 1221 correspondingly communicating with the first cavity 1211 to form a dropper groove 1201, and the material of the second pressing piece 122 is a rigid material. In this way, by communicating the dropper groove 1201 of the finger-pressing structure 120 with the external space of the chip, not only can the closed cavity design of the existing finger-pressing microfluidic chip be eliminated, but also the manufacturing cost can be saved and the volume of the finger-pressing transistor microfluidic chip 100 can be reduced. Furthermore, by making the first pressing plate 121 a cushioning material and the second pressing plate 122 a hard material, the hard second pressing plate 122 can not only limit the depth of the user pressing the first pressing plate 121, but also prevent the user's fingers from contacting the test liquid in the sample drop 111 when pressing the finger pressure structure 120, thereby making the use of the finger pressure transistor microfluidic chip 100 of the present invention safer and more convenient.

[0087] The printed circuit board 130 is fixedly mounted on the body 110 and includes a receiving slot 131. Specifically, the printed circuit board 130 connects to a detector and serves as a bridge for electrical transmission between the detector and the transistor chip 140. Figure 1 In the finger-pressure type transistor microfluidic chip 100, a printed circuit board 130 is disposed on a surface of the body 110 that is different from the finger-pressure structure 120, to prevent the user from accidentally damaging the printed circuit board 130 when applying force to the finger-pressure structure 120, thus affecting the subsequent detection results. Furthermore, the printed circuit board 130 can be a commercially available printed circuit board, or a printed circuit board with a special circuit configuration can be designed as needed, but the present invention is not limited thereto. Moreover, the printed circuit board 130 may also include a reference electrode 132, which is exposed in the sample dispensing port 111 to prevent contamination and improve detection efficiency.

[0088] A transistor chip 140 is embedded in a receiving groove 131 and electrically connected to a printed circuit board 130. The transistor chip 140 includes multiple working electrodes 141, and corresponds to a detection channel 112, exposing the working electrodes 141 within the detection channel 112 to detect the detection liquid or sample in the detection channel 112. Specifically, in the finger-pressure type transistor microfluidic chip 100 of the present invention, the transistor chip 140 can be an ion-sensitive field-effect transistor chip. An ion-sensitive field-effect transistor (ISFET) is a field-effect transistor chip used to measure the ion concentration in a solution. When the ion concentration changes, the current flowing through the ion-sensitive field-effect transistor chip changes accordingly, generating a corresponding electrical signal. This electrical signal can be further analyzed to achieve the detection purpose.

[0089] Please also refer to Figure 1 , Figure 2 and Figure 3 ,in Figure 3 Show Figure 1 Another exploded view of the finger-pressable transistor microchannel chip 100. (See diagram below.) Figures 1 to 3As shown, the body 110 includes a body surface 1101, and the body 110 is composed of an upper substrate 1102, a pipeline substrate 1103, and a bottom plate 1104 sequentially from the body surface 1101 downwards. The upper substrate 1102, pipeline substrate 1103, bottom plate 1104, and transistor chip 140 are sequentially stacked to form a detection channel 112, so that the working electrode 141 of transistor chip 140 is exposed in the detection channel 112 to detect the detection liquid or the sample to be tested. Furthermore, the upper substrate 1102 includes a first substrate opening 1105, and the pipeline substrate 1103 includes a first pipeline opening 1106. The first substrate opening 1105 is correspondingly connected to the first pipeline opening 1106, and the first substrate opening 1105, the first pipeline opening 1106, and bottom plate 1104 are sequentially stacked to form an absorption groove 114. Furthermore, the opening size of the first substrate opening 1105 can be smaller than the surface area of ​​the absorption pad 115 to prevent the absorption pad 115 from falling out of the first channel opening 1106 and to increase the efficiency of the absorption pad 115 in absorbing waste liquid. Additionally, the upper substrate 1102, the channel substrate 1103, and the base plate 1104 can be made of plastic or polymer materials to reduce the manufacturing cost of the finger-pressed transistor microfluidic chip 100 of the present invention and simplify its manufacturing process, and to enable mass production. Furthermore, the base plate 1104, the printed circuit board 130, and the transistor chip 140 are bonded to each other, and the base plate 1104 includes a reference electrode opening 1107, a chip electrode opening 1108, and two soldering groove openings 1109, wherein the reference electrode opening 1107 communicates with the sample dispensing port 111, and the chip electrode opening 1108 communicates with the detection channel 112.

[0090] Furthermore, in Figure 1 In the finger-pressure transistor microfluidic chip 100, the body 110 further includes two welding grooves 116, which are respectively disposed on both sides of the detection channel 112, and the openings 1109 of the welding grooves on the base plate 1104 are connected to the welding grooves 116. The welding grooves 116 are used to attach multiple electrodes 133 (marked on) of the printed circuit board 130. Figure 2 The circuit is soldered to the working electrode 141 of the transistor chip 140 to form an electrical conduction circuit, thereby allowing the slot (not shown) of the printed circuit board 130 to be connected to an external instrument.

[0091] Please refer to Figure 4 and Figure 5 , Figure 4 A schematic diagram of a finger-pressable transistor microchannel chip 200 according to another embodiment of the present invention is shown, while Figure 5 Show Figure 4 An exploded view of the finger-pressed transistor microfluidic chip 200. The finger-pressed transistor microfluidic chip 200 includes a body 210, a finger-pressed structure 220, a printed circuit board 230, and a transistor chip 240.

[0092] The main body 210 includes a sample drop port 211, a detection channel 212, a sample drop port 213, and an absorption tank 214. The sample drop port 213 is connected to the detection channel 212, while the absorption tank 214 includes an absorption pad (not shown in the figure).

[0093] A finger pressure structure 220 is disposed on the body 210 and has a sample dispensing groove 2201. The sample dispensing groove 2201 is connected to the sample dispensing port 211 and to an external space of a chip (not shown in the figure). The finger pressure structure 220 includes a first pressing piece 221 and a second pressing piece 222, with the second pressing piece 222 disposed between the body 210 and the first pressing piece 221. The first pressing piece 221 is made of a cushioning material, while the second pressing piece 222 is made of a hard material.

[0094] The printed circuit board 230 is fixedly disposed below the body 210 and includes a receiving groove 231 and a reference electrode 232, while the transistor chip 240 is embedded in the receiving groove 231 and is electrically connected to the printed circuit board 230.

[0095] in addition, Figure 4 The finger-pressable transistor microfluidic chip 200 and Figure 1 The structure and configuration of the finger-pressable transistor microfluidic chip 100 are largely similar. For details on the configuration of other identical or similar components, please refer to [reference needed]. Figure 1 The description of the finger-pressed transistor microfluidic chip 100 will not be repeated here.

[0096] like Figure 4 and Figure 5 As shown, in the finger pressure transistor microfluidic chip 200 of the present invention, the sample drop port 213 and the sample drop port 211 are disposed separately on the body 210, and the sample drop port 213 is connected to the detection channel 212 through a connecting channel 217, wherein the long axis of the detection channel 212 and the connecting channel 217 have an included angle θ, and the size of the included angle θ can be from 10° to 90°, or the size of the included angle θ can be 90°. In this way, by separating the sample dispensing port 213 and the dispensing port 211 on the body 210, and by connecting the sample dispensing port 213 to the detection channel 212 through the connecting channel 217, the opening size of the sample dispensing port 213 has a large margin, and the opening size of the sample dispensing port 213 can be increased as needed to facilitate direct dispensing with a dropper, thereby improving the versatility and ease of operation of the finger pressure transistor microchannel chip 200 of the present invention.

[0097] Furthermore, such as Figure 4As shown, the body 210 may further include a vent 218, which is correspondingly disposed on the detection channel 212 to control the volume of the detection liquid or the sample to be tested in the detection channel 212, and to ensure that the liquid covers the working electrode 241 above the transistor chip 240. Furthermore, the body 210 may also include a sealing film 219, which is detachably disposed on the surface 2101 of the body and seals the sample drop port 213 to prevent the detection liquid in the detection channel 212 from flowing to the sample drop port 213. When it is time to drop a sample, the sealing film 219 on the sample drop port 213 can be removed. However, the present invention is not limited thereto.

[0098] II. Operation method of the finger-pressable transistor microchannel chip of the present invention

[0099] Please refer to Figure 6 This diagram illustrates a flowchart of the operation method 300 for a pressure-sensitive transistor microfluidic chip according to another embodiment of the present invention. The operation method 300 for the pressure-sensitive transistor microfluidic chip includes steps 310, 320, 330, 340, 350, 360, and 370.

[0100] Step 310 involves providing a pressure-sensitive transistor microfluidic chip, which is electrically connected to a detector. Specifically, the operation method 300 of the pressure-sensitive transistor microfluidic chip of the present invention is used to operate the pressure-sensitive transistor microfluidic chip 100 or 200 of the present invention. For structural details of the pressure-sensitive transistor microfluidic chip 100 and 200 of the present invention, please refer to the preceding paragraph; they will not be repeated here.

[0101] Step 320 involves performing a first measurement step, in which a first detection solution is added to the sample dropper, allowing the first detection solution to enter through the sample dropper and fill the detection channel. Simultaneously, the user will further operate the detector to power on the printed circuit board and drive the transistor chip to measure a background value for each working electrode.

[0102] Step 330 involves a first pressing and emptying step, whereby pressing the finger pressure structure seals the sample collection tank, allowing the first detection liquid to drain from the detection channel and enter the absorption tank. Specifically, when the user presses the finger pressure structure, the finger covers and seals the sample collection tank, isolating it from the external space of the chip and forming a configuration similar to a closed cavity. Simultaneously, the user's pressing of the finger pressure structure changes the volume of the sample collection tank, correspondingly altering the internal pressure and creating a vacuum pump-like effect. This causes the first detection liquid to drain from the detection channel and enter the absorption tank, achieving multiple emptyings of the liquid within the detection channel and a cleaning effect. This allows the finger pressure transistor microchannel chip of the present invention to perform multiple repeated measurements.

[0103] Step 340 involves a sample reaction step, in which a sample to be tested is added to the sample dropper to fill the detection channel and react for a predetermined reaction time. During this process, the sample to be tested will not flow to the reference electrode in the dropper. The aforementioned predetermined reaction time will vary depending on the detection type and the type of sample to be tested, and may range from 1 minute to 30 minutes, but the present invention is not limited thereto.

[0104] Step 350 is to perform a second pressing and emptying step, in which the pressing finger structure is used to close the sample dropper, so that the sample to be tested is discharged from the detection channel and enters the absorption tank.

[0105] Step 360 involves a second measurement step, in which a second detection solution is added to the sample dropper, allowing the second detection solution to enter through the sample dropper and fill the detection channel. Simultaneously, the user further operates the detector to power on the printed circuit board and drive the transistor chip to measure a detection value at each working electrode. Furthermore, the amount of the first detection solution added is A, and the amount of the second detection solution added is B, satisfying the condition: 2A ≤ B. By adding a second detection solution at least twice the amount of the first detection solution, the remaining sample in the detection channel is flushed out by the excess second detection solution and absorbed by the absorption pad. The remaining second detection solution in the detection channel will further fill the detection channel for subsequent measurements.

[0106] Step 370 is a data analysis step, which analyzes the background value and detection value of each working electrode to obtain a detection result for each working electrode.

[0107] Furthermore, in the operation method 300 of the finger-pressure type transistor microfluidic chip of the present invention, the terms "first" and "second" in "first detection liquid" and "second detection liquid" are used for naming purposes and are not used to indicate quality or other meanings; this is hereby stated in advance. Moreover, in the present invention, the first detection liquid and the second detection liquid can be detection liquids of the same composition, or detection liquids of different compositions can be used as needed, but the present invention is not limited thereto.

[0108] Therefore, the operation method 300 of the finger-pressure transistor microfluidic chip of the present invention uses the finger-pressure transistor microfluidic chip of the present invention to test the sample. It can not only achieve the cleaning effect by repeatedly emptying the liquid in the test channel, but also enable the finger-pressure transistor microfluidic chip of the present invention to perform repeated measurements. This improves the detection accuracy and ease of use of the operation method of the finger-pressure transistor microfluidic chip of the present invention, and has application potential in related markets.

[0109] III. Operational Testing of the Finger-Pressure Transistor Microchannel Chip of the Present Invention

[0110] The following tests will be conducted using the finger-pressed transistor microchannel chip 400 of the first embodiment and the finger-pressed transistor microchannel chip 500 of the second embodiment of the present invention, respectively, in conjunction with the operation method 300 of the finger-pressed transistor microchannel chip of the present invention.

[0111] In detail, the finger-pressable transistor microchannel chip 400 of the first embodiment and Figure 1 The pressure-sensitive transistor microfluidic chip 100 is similar in structure and configuration to the first embodiment, while the pressure-sensitive transistor microfluidic chip 500 of the second embodiment is similar to... Figure 4 The pressure-sensitive transistor microfluidic chip 200 has a similar structure and configuration to the other two embodiments. The only difference is that the pressure-sensitive transistor microfluidic chip 400 in the first embodiment and the pressure-sensitive transistor microfluidic chip 500 in the second embodiment are mounted on an opaque substrate on a surface of the main body (not shown) that is different from the pressure-sensitive structures 420 and 520, and the detection channels 412 and 512 are closed to represent the configuration position of the printed circuit board and the transistor chip. This is to more clearly illustrate the delivery of the first detection liquid, the second detection liquid, and the test sample in the pressure-sensitive transistor microfluidic chip 400 and the pressure-sensitive transistor microfluidic chip 500. Therefore, for the configuration details of each component in the pressure-sensitive transistor microfluidic chip 400 of the first embodiment and the pressure-sensitive transistor microfluidic chip 500 of the second embodiment, please refer to the respective specifications. Figure 1 The finger-pressable transistor microfluidic chip 100 and Figure 4 The description of the pressure-sensitive transistor microfluidic chip 200 will not be repeated here. Furthermore, for details of each step of the operation method 300 of the pressure-sensitive transistor microfluidic chip, please refer to the description of the operation method 300 of the pressure-sensitive transistor microfluidic chip, which will also not be repeated here.

[0112] [First Embodiment]

[0113] Please refer to Figure 7 This illustrates a sequential operation diagram of a finger-pressable transistor microchannel chip 400 according to a first embodiment of the present invention. Furthermore, to enable... Figure 7 The operation of the finger-pressure transistor microfluidic chip 400 is clearer and easier to understand. Figure 9 To present Figure 7 A color illustration. In detail, Figure 7 From left to right, the images show the states of the finger-pressure transistor microfluidic chip 400 at different operating steps, as well as the distribution of the first detection liquid, the second detection liquid, and the sample to be tested in the finger-pressure transistor microfluidic chip 400 at different steps.

[0114] In the experiment, the finger-pressure transistor microfluidic chip 400 is first placed on an operating platform, and a first detection liquid (orange) is added to the sample groove 4201 of the finger-pressure structure 420, so that the first detection liquid enters the detection channel 412 through the sample outlet (not shown in the figure). At this time, if the finger-pressure transistor microfluidic chip 400 is electrically connected to a detector, the user can operate the detector to power on the printed circuit board and drive the transistor chip to measure a background value of each working electrode of the transistor chip.

[0115] Next, press the finger pressure structure 420 and close the sample dropper 4201 so that the first test liquid is discharged from the second end 4122 of the test channel 412 and enters the absorption tank 414. At this time, the first test liquid will be further absorbed by the absorption pad 415.

[0116] Then, the test sample (blue) is added to the sample dropper 413 so that the test sample fills the detection channel 412 and reacts for a predetermined reaction time.

[0117] Next, press the finger pressure structure 420 again and close the sample dropper 4201 so that the sample to be tested is discharged from the detection channel 412 and enters the absorption tank 414. At this time, the sample to be tested will be further absorbed by the absorption pad 415.

[0118] Finally, a second detection solution (orange) is added to the sample dropper 4201, allowing it to enter through the sample dropper 413 and fill the detection channel 412. The sample remaining in the detection channel 412 is flushed out by the excess second detection solution and flows into the absorption tank 414, where it is absorbed by the absorption pad 415. Simultaneously, if the finger-pressure transistor microfluidic chip 400 is electrically connected to a detector, the user will operate the detector to power the printed circuit board and drive the transistor chip to measure a detection value at each working electrode of the transistor chip. Then, the background value and detection value of each working electrode are collected to obtain a detection result for each working electrode.

[0119] [Second Embodiment]

[0120] Figure 8 A sequential operation diagram of a finger-pressable transistor microchannel chip 500 according to a second embodiment of the present invention is shown. Furthermore, to enable... Figure 8 The operation of the finger-pressable transistor microfluidic chip 800 is clearer and more understandable. Figure 10 To present Figure 8 A color illustration. In detail, Figure 8 From left to right, the images show the states of the finger-pressure transistor microfluidic chip 500 at different operating steps, as well as the distribution of the first detection liquid, the second detection liquid, and the sample to be tested in the finger-pressure transistor microfluidic chip 500 at different steps.

[0121] In the experiment, the finger-pressure transistor microfluidic chip 500 is first placed on an operating platform, and a first detection liquid (orange) is added to the sample dropper 5201 of the finger-pressure structure 520, so that the first detection liquid enters the detection channel 512 through the sample dropper (not shown in the figure). At this time, if the finger-pressure transistor microfluidic chip 500 is electrically connected to a detector, the user can operate the detector to power on the printed circuit board and drive the transistor chip to measure a background value of each working electrode of the transistor chip.

[0122] Next, press the finger pressure structure 520 and close the sample dropper 5201 so that the first test liquid is discharged from the second end 5122 of the test channel 512 and enters the absorption tank 514. At this time, the first test liquid will be further absorbed by the absorption pad 515.

[0123] Then, the sealing film above the sample drop port 513 is removed, and the sample to be tested (blue) is added to the sample drop port 513 so that the sample to be tested fills the detection channel 512 (green) through the connecting channel 517 and reacts for a predetermined reaction time.

[0124] Next, press the finger pressure structure 520 again and close the sample dropper 5201 so that the sample to be tested is discharged from the detection channel 512 and enters the absorption tank 514. At this time, the sample to be tested will be further absorbed by the absorption pad 515, while the sample to be tested in the connecting channel 517 will form a liquid barrier to prevent the sample to be tested in the detection channel 512 from flowing back to the sample dropper 513.

[0125] Finally, a second detection solution (orange) is added to the sample dropper 5201, allowing it to enter through the sample dropper and fill the detection channel 512. The sample remaining in the detection channel 512 will be flushed out by excess second detection solution and enter the absorption tank 514, where it will be absorbed by the absorption pad 515. The liquid barrier formed by the sample in the connecting channel 517 prevents excess second detection solution from flowing back to the sample dropper 513 and leaking out. If the finger-pressed transistor microfluidic chip 500 is electrically connected to the detector, the user will operate the detector to power the printed circuit board and drive the transistor chip to measure the detection value of each working electrode of the transistor chip. Then, the background value and detection value of each working electrode are collected to obtain the detection result for each working electrode.

[0126] In summary, the finger-pressure transistor microfluidic chip and its operation method of the present invention have the following advantages. First, the finger-pressure transistor microfluidic chip of the present invention, by communicating with the external space of the chip through the sample groove of the finger-pressure structure, not only eliminates the closed cavity design of existing finger-pressure microfluidic chips, but also saves manufacturing costs and reduces the size of the finger-pressure transistor microfluidic chip. Second, by using a first pressing plate made of a cushioning material and a second pressing plate made of a hard material, the hard second pressing plate not only limits the depth of the user's pressing of the first pressing plate, but also prevents the user's fingers from contacting the liquid in the sample outlet during pressing, thereby making the use of the finger-pressure transistor microfluidic chip of the present invention safer and more convenient. Third, by setting an absorption pad in the absorption groove, waste liquid discharged from the detection channel can be absorbed, avoiding waste liquid contamination or leakage, thereby improving the detection accuracy and ease of use of the finger-pressure transistor microfluidic chip of the present invention. Fourth, the operation method of the finger-pressure transistor microfluidic chip of the present invention, which uses the finger-pressure transistor microfluidic chip of the present invention to test the sample, can repeatedly empty the liquid in the test channel to achieve a cleaning effect, so that the finger-pressure transistor microfluidic chip of the present invention can perform repeated measurements, thereby improving the detection accuracy and ease of use of the operation method of the finger-pressure transistor microfluidic chip of the present invention, and has application potential in related markets.

[0127] Although the present invention has been disclosed above with reference to embodiments, it is not intended to limit the present invention. Any person skilled in the art can make various changes and modifications without departing from the concept and scope of the present invention. Therefore, the scope of protection of the present invention shall be determined by the claims.

Claims

1. A finger-pressable transistor microchannel chip, characterized in that, Include: One entity, comprising: A single drop sample; A detection channel having a first end and a second end, wherein the first end is in communication with the sample droplet; One sample dropper, the tubing is connected to the detection channel; and An absorption groove is connected to the second end of the detection flow channel, wherein the absorption groove includes an absorption pad; A finger-pressure structure is disposed on the body and has a sample groove, wherein the sample groove is connected to the sample outlet and is connected to an external space of a chip, and the finger-pressure structure includes: A first pressing piece, comprising a first cavity, wherein the first pressing piece is made of a cushioning material; and A second pressing piece is disposed between the body and the first pressing piece, wherein the second pressing piece includes a second cavity, the second cavity correspondingly communicates with the first cavity to form the droplet groove, and the material of the second pressing piece is a hard material; A printed circuit board, fixedly mounted on the body and including a receiving groove; and A transistor chip is embedded in the receiving slot and electrically connected to the printed circuit board, wherein the transistor chip includes multiple working electrodes and the transistor chip corresponds to the detection channel and exposes the working electrodes in the detection channel.

2. The finger-pressed transistor microchannel chip as claimed in claim 1, wherein the printed circuit board is disposed on a surface of the body that is different from the finger-pressed structure.

3. The finger-pressable transistor microchannel chip of claim 2, wherein the printed circuit board further includes a reference electrode exposed in the sample dropper.

4. The finger-pressable transistor microchannel chip as described in claim 1, wherein the transistor chip is an ion-sensitive field-effect transistor chip.

5. The finger-pressable transistor microchannel chip as claimed in claim 1, wherein the body further comprises: Two welding grooves are respectively disposed on both sides of the detection flow channel. The two welding grooves are used to weld multiple electrodes of the printed circuit board to the working electrodes of the transistor chip to form an electrical conduction circuit.

6. The finger-pressable transistor microchannel chip as claimed in claim 1, wherein the first pressing piece is made of foam, silicone, rubber, or a combination thereof.

7. The finger-pressable transistor microchannel chip of claim 1, wherein the sample droplet is opened on the detection channel and located on the side of the detection channel near the second end.

8. The finger-pressure transistor microchannel chip as claimed in claim 1, wherein the sample dispensing port is disposed separately from the body, and the sample dispensing port is connected to the detection channel through a connecting channel.

9. The finger-pressable transistor microchannel chip of claim 8, wherein the long axis of the detection channel and the connecting channel have an angle between them, and the angle is between 10° and 90°.

10. The finger-pressable transistor microchannel chip of claim 1, wherein the body includes a body surface, and the body is composed of an upper substrate, a pipeline substrate and a bottom plate in sequence from the body surface downwards. in, The upper substrate, the pipeline substrate, the bottom plate, and the transistor chip are stacked sequentially to form the detection flow channel; The upper substrate includes a first substrate opening, the pipeline substrate includes a first pipeline opening, the first substrate opening is connected to the first pipeline opening, and the first substrate opening, the first pipeline opening and the bottom plate are stacked in sequence to form the absorption groove.

11. The finger-pressable transistor microchannel chip of claim 10, wherein the opening size of the first substrate opening is smaller than the surface area of ​​the absorbent pad.

12. The finger-pressable transistor microchannel chip of claim 10, wherein the substrate, the printed circuit board and the transistor chip are bonded to each other, and the substrate includes a reference electrode opening, a chip electrode opening and two solder groove openings.

13. A method for operating a finger-pressable transistor microfluidic chip, characterized in that, Include: A pressure-sensitive transistor microfluidic chip as described in claim 1 is provided, wherein the pressure-sensitive transistor microfluidic chip is electrically connected to a detector; A first measurement step is performed, in which a first detection liquid is added to the sample trough so that the first detection liquid is input from the sample port and fills the detection channel. At this time, the detector is operated to power on the printed circuit board and drive the transistor chip to measure a background value of each working electrode. A first pressing and emptying step is performed, in which the finger pressure structure is pressed and the sample dropper is closed, so that the first detection liquid is discharged from the detection channel and enters the absorption tank; A sample reaction step is performed, in which a sample to be tested is added to the sample dropper so that the sample to be tested fills the detection channel and reacts for a predetermined reaction time. A second pressing and emptying step is performed, in which the finger pressure structure is pressed and the sample dropper is closed, so that the sample to be tested is discharged from the detection channel and enters the absorption tank. A second measurement step is performed, in which a second detection solution is added to the sample dropper, so that the second detection solution enters from the sample dropper and fills the detection channel. At this time, the detector is operated to power on the printed circuit board and drive the transistor chip to measure a detection value of each working electrode; and A data analysis step is performed to analyze the background value and the detection value of each working electrode to obtain a detection result for each working electrode.

14. The method of operating the finger-pressable transistor microfluidic chip as described in claim 13, wherein the amount of the first detection liquid added is A, and the amount of the second detection liquid added is B, which satisfies the following condition: 2A≤B.

15. The method of operating the finger-pressed transistor microchannel chip as claimed in claim 13, wherein the printed circuit board is disposed on a surface of the body that is different from the finger-pressed structure.

16. The method of operating the finger-pressable transistor microchannel chip as claimed in claim 13, wherein the printed circuit board further includes a reference electrode exposed in the sample dropper.

17. The method of operating the finger-pressed transistor microchannel chip as described in claim 13, wherein the transistor chip is an ion-sensitive field-effect transistor chip.

18. The method of operating the finger-pressed transistor microchannel chip as described in claim 13, wherein the material of the first pressing piece is foam, silicone, rubber, or a combination thereof.

19. The method of operating the finger-pressed transistor microchannel chip as claimed in claim 13, wherein the sample droplet is opened on the detection channel and located on the side of the detection channel near the second end.

20. The method of operating the finger-pressed transistor microchannel chip as described in claim 13, wherein the sample dispensing port and the sample dispensing port are disposed separately on the body, and the sample dispensing port is connected to the detection channel through a connecting channel.

21. The method of operating the finger-pressed transistor microchannel chip as described in claim 20, wherein the long axis of the detection channel and the connecting channel have an angle between them, and the angle is between 10° and 90°.

Images