Anti-wrinkle composition, essence containing the same, and preparation method

Abstract

The present invention relates to the field of cosmetics, specifically to an anti-wrinkle composition, an essence containing the composition, and a preparation method. The anti-wrinkle composition comprises arginine / lysine peptides, a chordate extract, and dipalmitoyl hydroxyproline in a mass ratio of 0.05-2:0.5-1.5:0.5-1.5. Compared to existing technologies, the present invention utilizes the active ingredients conopeptide macromolecules and chordate extract to rebuild the epidermis and stimulate collagen expression. Dipalmitoyl hydroxyproline scavenges free radicals and reduces skin inflammation, achieving anti-wrinkle effects from multiple perspectives. Liquid crystal encapsulation enhances its permeability, allowing it to remain in targeted skin locations. The essence also exhibits excellent biocompatibility and is non-toxic and non-irritating. The essence has strong skin penetration and exhibits significant anti-wrinkle effects across the face.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, in particular to an anti-wrinkle composition, an essence containing the composition and a preparation method. Background Art

[0002] Currently, there are a wide variety of anti-wrinkle products on the market. However, anti-wrinkle masks, for example, only stay on the face for a short time, have little effect, require long-term use, and are inconvenient to use. Anti-wrinkle toners and creams contain relatively low levels of active ingredients, resulting in insignificant anti-wrinkle effects.

[0003] Most anti-wrinkle products on the market still have various defects such as poor permeability and high irritation. Long-term use of some raw materials such as retinoic acid, although accelerating cell division, shortens cell lifespan and accelerates the formation of wrinkles. Therefore, providing skin care products that are easier to absorb, have better anti-wrinkle performance and are safer is a technical problem that urgently needs to be solved.

[0004] Patent application CN202010290222.7 discloses a polypeptide anti-wrinkle and anti-aging essence, which is composed of the following components by mass percentage: carnosine 0.5-2%, snake venom tripeptide 0.5-5%, acetyl hexapeptide-8 0.5-4%, oligopeptide-1 0.1-2%, dipalmitoyl hydroxyproline 1-4%, deionized water 80.5-88%, betaine 0.5-3%, parahydroxyacetophenone 0.1-0.5%, xanthan gum 0.1-0.2%, butylene glycol 0.2-4%, sodium hyaluronate 0.1-1%, oat beta-glucan 0.5-2%, and 1.2-hexanediol 0.1-2%. The invented peptide-based anti-wrinkle and anti-aging essence can make the skin, especially the skin around the eyes, lastingly moisturized, comprehensively replenish cell nutrition, and slow down the formation of true wrinkles around the eyes. However, the essence achieves its anti-aging effect by adding multiple peptides, and its mode of action, principle and improvement of skin condition are relatively simple, resulting in poor results. Summary of the Invention

[0005] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and provide an anti-wrinkle composition that is easily absorbed, has better anti-wrinkle performance and is safe, as well as an essence containing the composition and a preparation method.

[0006] The purpose of the present invention can be achieved through the following technical scheme: an anti-wrinkle composition, comprising arginine / lysine polypeptide, galangal extract and dipalmitoyl hydroxyproline in a mass ratio of 0.05-2:0.5-1.5:0.5-1.5.

[0007] Furthermore, the mass ratio of the arginine / lysine polypeptide, the chordate extract and dipalmitoylhydroxyproline is 0.05-0.1:1:1.

[0008] The present invention selects conopeptide, scutellaria extract and dipalmitoyl hydroxyproline composition as active ingredients.

[0009] Among them, arginine / lysine peptide is also called conopeptide, which has the characteristics of small relative molecular weight, high activity, high selectivity and easy synthesis. It is called smearable botulinum toxin. It can relax muscles very effectively without paralyzing muscle nerve conduction, achieving the effect of instant wrinkle removal. Long-term application can fade fine lines and delay skin aging.

[0010] Dipalmitoylhydroxyproline can stimulate the contraction of collagen fibers, capture free radicals, protect skin cells, inhibit the activity of MMPs, protect collagen and DEJ structure, and has a stronger effect than vitamin C in stimulating collagen fiber contraction and inhibiting elastase activity. It has ideal skin affinity and restores skin firmness.

[0011] Corallina serrata is a calcified algae that contains the primary acidic polysaccharide, corallin, as well as several free amino acids, fatty acids, and cholesterol. It is also rich in vitamins C and B1. Corallina serrata extract restores skin elasticity by promoting the synthesis of proteoglycans in the dermis. It also improves the stratum corneum and rebuilds the epidermis by promoting the expression of epidermal keratin K14. It also promotes collagen synthesis, reducing deep and superficial wrinkles and firming the skin. It also inhibits the activity of hyaluronidase and collagenase.

[0012] This treatment combines conopeptides, chordate extract, and dipalmitoyl hydroxyproline. Conopeptides specifically block voltage-dependent sodium channels, effectively relaxing the skin's inner muscles; dipalmitoyl hydroxyproline stimulates the contraction of collagen fibers in the dermis, restoring skin firmness; and chordate extract promotes collagen synthesis, reducing wrinkles. These three ingredients work synergistically to tighten the skin on multiple levels, both internally and externally, achieving superior wrinkle-reducing results.

[0013] The present application also provides an essence containing the anti-wrinkle composition, comprising the following components in percentage by weight: phase A, phase B, phase C and phase D;

[0014] The phase A includes the following components by mass fraction:

[0015] Plant lecithin 0.2-5%;

[0016] Higher fatty alcohol 0.2-10%;

[0017] Phytosterol fatty acid esters 0.1-8.5%;

[0018] The phase B includes the following components by mass fraction:

[0019] Sodium hyaluronate 0.1-0.2%;

[0020] Glycerol 4-6%;

[0021] Carbomer 0.12-2%;

[0022] 1,2-propylene glycol 1.5-2.5%;

[0023] Squalane 1.2-2.5%;

[0024] Xanthan gum 0.04-0.08%;

[0025] Caprylic / capric glyceride 0.5-1%;

[0026] 1,2-Hexanediol 1.5-2.4%;

[0027] The C phase includes the following components by mass fraction:

[0028] Phenoxyethanol 0.5-1%;

[0029] Sodium bicarbonate 0.5-0.9%;

[0030] Sodium dihydrogen carbonate buffer 5-15%;

[0031] Flavor 0.1-1%;

[0032] Deionized water;

[0033] The D phase includes the following components by mass fraction:

[0034] Arginine / lysine peptide 0.1-2%;

[0035] Coriander leaf extract 0.5-1.5%;

[0036] Dipalmitoylhydroxyproline 0.5-1.5%.

[0037] Furthermore, the phase A includes the following components by mass fraction:

[0038] Plant lecithin 1-3%;

[0039] Higher fatty alcohol 2-6%;

[0040] Phytosterol fatty acid esters 1-3%.

[0041] Furthermore, the phase B includes the following components by mass fraction:

[0042] Sodium hyaluronate 0.12-0.18%;

[0043] Glycerol 4.5-5.5%;

[0044] Carbomer 0.15-0.2%;

[0045] 1,2-propylene glycol 1.8-2.2%;

[0046] Squalane 1.8-2.2%;

[0047] Xanthan gum 0.05-0.07%;

[0048] Caprylic / capric glyceride 0.6-1%;

[0049] 1,2-Hexanediol 1.8~2.2%.

[0050] Furthermore, the C phase includes the following components by mass fraction:

[0051] Phenoxyethanol 0.5-1%;

[0052] Sodium bicarbonate 0.6-0.8%;

[0053] Sodium dihydrogen carbonate buffer 8-12%;

[0054] Fragrance 0.1~0.3%

[0055] Deionized water.

[0056] The present application also provides a method for preparing the essence, comprising the following steps:

[0057] (1) Preparation of oil phase: Weigh all components of phase B, place them in a water bath at 75-80°C and stir slowly for 10-60 min until all are dissolved to obtain a uniform mixture a.

[0058] (2) After the mixture a is cooled, add the raw materials of phase D and stir homogeneously;

[0059] (3) Add the raw materials of phase A and stir homogenously for 10 to 60 minutes to obtain mixture b.

[0060] (4) Then, the raw materials of phase C are added to the mixture b, the fragrance and pH are adjusted, and the mixture is stirred evenly to obtain the essence.

[0061] Furthermore, the speed of slow stirring in step (1) is 40-60 r / min.

[0062] Furthermore, the speed of homogenizing and stirring in step (2) and step (3) is 1500-2500 r / min.

[0063] Furthermore, in step (4), the pH value is adjusted to 6.0-7.0, and sodium bicarbonate and sodium dihydrogen carbonate buffer are used to adjust the pH.

[0064] Liquid crystals are a special state of matter between solid and liquid, with a fixed crystal order and the flow characteristics of a liquid. In the cosmetics field, liquid crystals have high bioadhesion, physical stability, self-thickening properties, can simultaneously carry hydrophilic and lipophilic functional ingredients, and reduce ultraviolet damage to the skin. The present invention forms a lamellar liquid crystal by combining specific amounts of plant lecithin, higher fatty alcohols, and plant sterol fatty acid esters to form a natural liquid crystal emulsifier, which is then mixed with active ingredients such as conopeptide to form a lamellar liquid crystal. Specific ingredients such as sodium hyaluronate, carbomer, squalane, xanthan gum, and caprylic / capric glyceride constitute skin conditioners and moisturizers, while phenoxyethanol, sodium bicarbonate, and sodium dihydrogen carbonate buffer constitute preservatives and pH regulators. This lamellar liquid crystal can encapsulate the active ingredients conopeptide, sucralose extract, and dipalmitoylhydroxyproline, preventing them from being structurally damaged or destroyed by physical or chemical processes, thereby promoting their penetration into the dermis and producing a good wrinkle-reducing effect.

[0065] Compared with the prior art, the present invention has the following beneficial effects:

[0066] 1. The present invention uses conopeptide, scutellaria extract and dipalmitoyl hydroxyproline as active ingredients, which is safe, mild, non-irritating, and has significant anti-wrinkle effects.

[0067] 2. The present invention adopts liquid crystal technology, which has better adaptability to human tissue and good permeability and moisture retention than ordinary essence skin care products. It also has a comfortable skin feel, transparent and beautiful color, and is safe and reliable. It can improve the stability of active ingredients, encapsulate active ingredients such as conopeptide to prevent physical and chemical degradation, promote its arrival in the dermis, and play a good wrinkle-removing effect. The active ingredient conopeptide macromolecule is encapsulated by liquid crystal technology to improve its stability, and it itself has good biocompatibility and is non-toxic and non-irritating.

[0068] 3. The essence with anti-wrinkle effect of the present invention has strong skin permeability, can reduce wrinkles, improve skin firmness, and repair skin barrier, thereby playing an effective anti-aging, hydrating and moisturizing effect.

[0069] 4. The essence provided by the present invention is simple to prepare, with a clear process and readily available raw materials. BRIEF DESCRIPTION OF THE DRAWINGS

[0070] Figure 1 This is a statistical graph of skin wrinkle (forehead) feature counts;

[0071] Figure 2 This is a statistical chart of skin wrinkle (forehead) scores;

[0072] Figure 3 This is the percentile statistical chart of skin wrinkles (forehead);

[0073] Figure 4This is a statistical chart of skin wrinkle (under the eyes) feature counts;

[0074] Figure 5 This is a statistical chart of skin wrinkle (under the eyes) scores;

[0075] Figure 6 This is a statistical chart of percentiles of skin wrinkles (under the eyes);

[0076] Figure 7 This is a statistical chart of the average depth of skin wrinkles (forehead);

[0077] Figure 8 This is a statistical chart of the number of skin wrinkles (forehead);

[0078] Figure 9 This is a statistical chart of skin wrinkle (forehead) volume;

[0079] Figure 10 This is a statistical chart of skin wrinkle (forehead) area;

[0080] Figure 11 This is a statistical chart of skin wrinkle (forehead) length;

[0081] Figure 12 This is a statistical chart of Ra values ​​for skin wrinkles (forehead);

[0082] Figure 13 This is a statistical chart of the average depth of skin wrinkles (outer canthus);

[0083] Figure 14 This is a statistical chart of the number of skin wrinkles (outer canthus);

[0084] Figure 15 This is a statistical diagram of the volume of skin wrinkles (outer canthus);

[0085] Figure 16 This is a statistical chart of the area of ​​skin wrinkles (outer canthus);

[0086] Figure 17 This is a statistical chart of the length of skin wrinkles (outer canthus);

[0087] Figure 18 This is a statistical chart of Ra values ​​for skin wrinkles (outer canthus);

[0088] Figure 19 This is the original image of Primos CR outer canthus wrinkles;

[0089] Figure 20 This is the Primos CR outer canthus wrinkle analysis chart: bright colors represent raised areas, and dark colors represent sunken areas;

[0090] Figure 21 This is a statistical chart of the average depth of skin wrinkles (nasolabial folds);

[0091] Figure 22 This is a statistical chart of the number of skin wrinkles (nasolabial folds);

[0092] Figure 23 This is a statistical diagram of the volume of skin wrinkles (nasolabial folds);

[0093] Figure 24 This is a statistical chart of the area of ​​skin wrinkles (nasolabial folds);

[0094] Figure 25 This is a statistical chart of the length of skin wrinkles (nasolabial folds);

[0095] Figure 26 This is a statistical chart of Ra values ​​for skin wrinkles (nasolabial folds);

[0096] Figure 27 This is a comparison chart of the mouse skin retention of Examples 1-3 and Comparative Examples 1-5. DETAILED DESCRIPTION

[0097] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0098] The raw materials used in the present invention are all commercially available products, such as

[0099] Arginine / lysine peptides can be selected from commercially available products such as Nanjing Leon Biotechnology Co., Ltd. and Chengdu Yunxi Chemical Co., Ltd.;

[0100] The extract of the fork coral algae can be selected from commercial products such as Ashland;

[0101] Dipalmitoylhydroxyproline can be selected from commercially available products such as Hubei Xinrunde Chemical Co., Ltd. and Sichuan Jisheng Biopharmaceutical Co., Ltd.;

[0102] Plant lecithin can be selected from commercially available products such as Mufan Biotechnology Co., Ltd. and Hebei Meiye Siwei Biotechnology Co., Ltd.;

[0103] Higher fatty alcohols can be selected from commercially available products such as Zhenwei Biotechnology Co., Ltd. and Shengtao Biotechnology Co., Ltd.;

[0104] Phytosterol fatty acid esters can be selected from commercially available products such as Xi'an Haisifu Biotechnology Co., Ltd. and Shaanxi Xinrunhao Biotechnology Co., Ltd.;

[0105] Examples 1-3 and Comparative Examples 1-4

[0106] The essence containing the anti-wrinkle composition is prepared according to the weight percentage ratio in Table 1:

[0107] Table 1 Ratio of raw material components of Example 1 and Comparative Examples 1-4

[0108]

[0109]

[0110] The preparation process of the essence of Examples 1-3 of the present invention is as follows:

[0111] (1) Preparation of oil phase: According to the components of phase B in Table 1, weigh the corresponding mass of each substance, place it in a water bath at 75-80°C and stir slowly for about 30 minutes (stirring speed 50 r / min) until it is completely dissolved to obtain a uniform mixture a;

[0112] (2) After the mixture a is cooled, add the raw materials of phase D and stir homogenously (stirring speed 2000 r / min);

[0113] (3) Add all the raw materials of phase A and stir homogenously for about 30 minutes (stirring speed 2000 r / min) to obtain mixture b;

[0114] (4) Then, the raw materials of phase C are added to the mixture b, the fragrance and pH are adjusted to 6.0-7.0, and the mixture is stirred evenly to obtain the essence.

[0115] The preparation process of the essence of Comparative Examples 1-4 is as follows:

[0116] (1) Preparation of oil phase: According to the components of phase B in Table 1, weigh the corresponding mass of each substance, place it in a water bath at 75-80°C and stir slowly for about 30 minutes (stirring speed 50 r / min) until it is completely dissolved to obtain a uniform mixture C.

[0117] (2) After the mixture C is cooled, add the raw materials of phase D and stir homogeneously (stirring speed 2000r / min).

[0118] (3) Then add the raw materials of phase A and stir homogenously for about 30 minutes (stirring speed 2000 r / min) to obtain mixture d.

[0119] (4) Then, various raw materials of phase C are added to the mixture d, the fragrance and pH are adjusted to 6.0-7.0, and the mixture is stirred evenly to obtain the essence.

[0120] The performance of the essence obtained in each embodiment and comparative example was tested as follows:

[0121] The study subjects were 80 healthy Chinese women with sensitive skin, aged between 27 and 48 years (mean age 36.90 ± 5.24 years). They presented with crow's feet / fine lines, under-eye wrinkles / fine lines, dark circles / eye bags, and ptosis. The subjects provided informed written consent. The subjects were willing to cooperate and understood the necessity and duration of the control, in order to adhere to the protocol established by the clinical trial center. The 80 subjects were divided into 8 groups of 10 each. Each group used Examples 1-3 and Comparative Examples 1-5, once daily in the morning and evening for 28 consecutive days. VISIA and Primos CR images were taken by the experimenter on Day 0, Day 14, and Day 28, and the subjects completed a self-assessment questionnaire. Data were analyzed using SPSS 28.0. Normal distribution tests were performed on the test data. If the test data were normally distributed, the T-test was used for statistical analysis; if the test data were non-normally distributed, the rank sum test was used for statistical analysis. Rank sum tests were used for statistical analysis of ranked data. The statistical significance level was p < 0.05.

[0122] 1. Skin wrinkle test results (forehead)

[0123] VISIA was used to collect and analyze facial image information. Feature counting counts feature parameters, ignoring their size and intensity. A smaller value indicates improved skin. Score evaluates the size, area, and intensity of feature parameters. A smaller score indicates improved skin.

[0124] The above method was used to test the essence obtained in Example 1. Figure 1-3 As shown, compared with the average of 10 subjects before using the sample, after using the sample for 14 days, the number of forehead skin wrinkle features decreased by 10.29%, the skin wrinkle score decreased by 7.13%, and the skin wrinkle percentile decreased by 4.50%. After using the sample for 28 days, the number of forehead skin wrinkle features decreased by 14.18%, the skin wrinkle score decreased by 15.54%, and the skin wrinkle percentile decreased by 7.90%.

[0125] 2. Skin wrinkles (under the eyes)

[0126] VISIA was used to collect and analyze facial image information. Feature counting counts feature parameters, ignoring their size and intensity. A smaller value indicates improved skin. Score evaluates the size, area, and intensity of feature parameters. A smaller score indicates improved skin.

[0127] like Figure 4-6As shown (average of 10 subjects), after 14 days of using the sample (i.e., the essence obtained in Example 1), the number of characteristic wrinkles under the eyes decreased by 5.80%, the wrinkle score decreased by 13.81%, and the wrinkle percentile decreased by 13.30%. After 28 days of using the sample, the number of characteristic wrinkles under the eyes decreased by 7.03%, the wrinkle score decreased by 14.89%, and the wrinkle percentile decreased by 18.15%.

[0128] 3. Skin wrinkles (forehead)

[0129] Primos CR is used to collect skin wrinkles and analyze photos of facial images. A smaller analysis value indicates that skin wrinkles have improved.

[0130] like Figure 7-12 As shown (average value of 10 subjects), compared with before using the sample, after using the sample (i.e., the essence obtained in Example 1) for 14 days, the average depth of skin wrinkles on the forehead was significantly reduced by 2.42%; the number of skin wrinkles was significantly reduced by 21.59%; the volume of skin wrinkles was significantly reduced by 19.01%; the area of ​​skin wrinkles was significantly reduced by 25.46%; the length of skin wrinkles was significantly reduced by 22.61%; and the Ra value of skin wrinkles was significantly reduced by 7.43%. After using the sample for 28 days, the average depth of skin wrinkles on the forehead was significantly reduced by 24.61%; the number of skin wrinkles was significantly reduced by 27.25%; the volume of skin wrinkles was significantly reduced by 25.62%; the area of ​​skin wrinkles was significantly reduced by 27.23%; the length of skin wrinkles was significantly reduced by 25.94%; and the Ra value of skin wrinkles was significantly reduced by 12.93%. The specific test results of each embodiment and comparative example are shown in Table 2 below:

[0131] Table 2

[0132]

[0133]

[0134] 4. Skin wrinkles (outer canthus)

[0135] Primos CR is used to collect skin wrinkles and analyze photos of facial images. A smaller analysis value indicates that skin wrinkles have improved.

[0136] like Figure 13-18As shown, compared with before using the sample, after using the sample (i.e., the essence obtained in Example 1) for 14 days, the average depth of skin wrinkles at the outer corners of the eyes was significantly reduced by 5.67%; the number of skin wrinkles was significantly reduced by 24.04%; the volume of skin wrinkles was significantly reduced by 19.70%; the area of ​​skin wrinkles was significantly reduced by 17.43%; the length of skin wrinkles was significantly reduced by 18.36%; and the Ra value of skin wrinkles was significantly reduced by 7.06%. After using the sample for 28 days, the average depth of skin wrinkles at the outer corners of the eyes was significantly reduced by 7.64%; the number of skin wrinkles was significantly reduced by 27.32%; the volume of skin wrinkles was significantly reduced by 30.30%; the area of ​​skin wrinkles was significantly reduced by 20.93%; the length of skin wrinkles was significantly reduced by 23.48%; and the Ra value of skin wrinkles was significantly reduced by 7.70%. The specific test results of each embodiment and comparative example are shown in Table 3 below:

[0137] Table 3

[0138]

[0139]

[0140] like Figure 19 Shown is the original image of Primos CR outer canthus wrinkles (for one of the 80 research subjects mentioned above): As can be seen from the figure, compared with before use of the sample, the outer canthus skin wrinkles were improved after 14 days of use of the sample (i.e., the essence obtained in Example 1); after 28 days, the skin wrinkles were significantly improved.

[0141] like Figure 20 The following is a Primos CR outer canthus wrinkle analysis diagram (with Figure 19 The research subjects in the figure are the same person). In the figure, bright colors represent raised areas, and dark colors represent sunken areas. It can be seen from the figure that compared with before use of the sample, the ratio of light to dark colors was significantly reduced after 14 days of use of the sample (i.e., the essence obtained in Example 1), indicating that the wrinkles on the outer canthus skin were significantly improved. After 28 days, the skin wrinkles were significantly improved compared with before use.

[0142] 5. Skin wrinkles (nasolabial folds)

[0143] Primos CR is used to collect skin wrinkles and analyze photos of facial images. A smaller analysis value indicates that skin wrinkles have improved.

[0144] like Figure 21-26As shown, compared with before use of the sample, after using the sample (i.e., the essence obtained in Example 1) for 14 days, the average depth of skin wrinkles in the nasolabial folds was significantly reduced by 1.97%; the number of skin wrinkles remained unchanged; the volume of skin wrinkles was significantly reduced by 4.26%; the area of ​​skin wrinkles was significantly reduced by 8.48%; the length of skin wrinkles was significantly reduced by 11.78%; and the Ra value of skin wrinkles was significantly reduced by 4.02%. After using the sample for 28 days, the average depth of skin wrinkles in the nasolabial folds was significantly reduced by 2.35%; the number of skin wrinkles remained unchanged; the volume of skin wrinkles was significantly reduced by 6.16%; the area of ​​skin wrinkles was significantly reduced by 8.60%; the length of skin wrinkles was significantly reduced by 12.41%; and the Ra value of skin wrinkles was not significantly reduced by 4.61%. The specific test results of each embodiment and comparative example are shown in Table 4 below:

[0145] Table 4

[0146]

[0147]

[0148] 6. In vitro permeability test

[0149] Take healthy SPF level male mice, the quantity of a batch of purchase is 24, after mice is carried out cervical dislocation and is put to death, cuts off abdominal skin, removes fat layer, scrapes the hair of mouse epidermis, cleans with normal saline, and is uniformly preserved.Randomly select mouse skin and ensure that mouse skin thickness is close, after being selected, it is fixed between diffusion cell and receiving cell, the dermis layer of skin faces receiving cell, and keratin layer faces diffusion cell, and the volume of receiving cell is 15mL, and skin dermis layer will just contact with the solvent in receiving cell, to prevent bubble from producing.Receiving solution is 50% ethanol water, adds 1g sample (embodiment 1-3 and comparative example 1-5) in supply cell, and receiving cell is placed on the magnetic stirring apparatus of intelligent transdermal experimental instrument, arranging water bath temperature is 37 DEG C, and stirring velocity is 350r / min, and transdermal time is 12h. After the experiment, the skin was removed, and the surface of the mouse skin was wiped and rinsed with 50% ethanol aqueous solution. After being placed on filter paper to dry at room temperature, the mouse skin was moved to a small beaker, cut into pieces with scissors, and added with 50% ethanol aqueous solution. Ultrasonic extraction was performed for 30 minutes and soaked for 24 hours. The extract was filtered through a 0.22μm microporous filter membrane and then subjected to chromatographic analysis to calculate the skin retention rate of different samples.

[0150] Skin retention Q (μg / cm 2 ), the calculation formula is Q = Vc / A, where A is the effective diffusion area, V is the total volume of the skin extract, and c is the mass concentration of the sample in the skin extract.

[0151] like Figure 27As shown, it is a comparison chart of the mouse skin retention of Example 1-3 and Comparative Example 1-5. From the experimental results, it can be seen that the transdermal performance of Example 1-3 is stronger than that of Comparative Example 1-5, showing a stronger skin retention ability.

Claims

1. An essence, characterized in that: The raw materials of the essence include the following components in percentage by weight: Phase A, Phase B, Phase C and Phase D; with the total weight of each raw material being 100%: The phase A includes the following components by mass fraction: Plant lecithin 0.2~5%; Higher fatty alcohol 0.2~10%; Phytosterol fatty acid esters 0.1~8.5%; The phase B includes the following components by mass fraction: Sodium hyaluronate 0.1~0.2%; Glycerol 4~6%; Carbomer 0.12~2%; 1,2-propylene glycol 1.5~2.5%; Squalane 1.2~2.5%; Xanthan gum 0.04~0.08%; Caprylic / capric glyceride 0.5~1%; 1,2-hexanediol 1.5~2.4%; The C phase includes the following components by mass fraction: Phenoxyethanol 0.5~1%; Sodium bicarbonate 0.5-0.9%; Sodium dihydrogen carbonate buffer 5-15%; Fragrance 0.1~1%; Deionized water; The D phase includes the following components by mass fraction: Arginine / lysine peptide 0.05~2%; 0.5~1.5% of galangal extract; Dipalmitoylhydroxyproline 0.5~1.5%.

2. The essence according to claim 1, characterized in that The phase A includes the following components by mass fraction: Plant lecithin 1~3%; Higher fatty alcohol 2~6%; Phytosterol fatty acid esters 1~3%.

3. The essence according to claim 1, characterized in that The phase B includes the following components by mass fraction: Sodium hyaluronate 0.12~0.18%; Glycerol 4.5~5.5%; Carbomer 0.15~0.2%; 1,2-propylene glycol 1.8 ~2.2%; Squalane 1.8~2.2%; Xanthan gum 0.05~0.07%; Caprylic / capric glyceride 0.6~1%; 1,2-Hexanediol 1.8~2.2%.

4. The essence according to claim 1, characterized in that The C phase includes the following components by mass fraction: Phenoxyethanol 0.5~1%; Sodium bicarbonate 0.6~0.8%; Sodium dihydrogen carbonate buffer 8-12%; Flavor 0.1~0.3%.

5. The method for preparing the essence according to claim 1, wherein: The following steps are involved: (1) Preparation of oil phase: Weigh each component of phase B and place it in a water bath at 75-80°C and stir slowly for 10-60 min until all components are dissolved to obtain a uniform mixture a. (2) After the mixture a is cooled, add the raw materials of phase D and stir evenly; (3) Add the raw materials of phase A and stir at a constant speed for 10-60 min to obtain mixture b; (4) Then, the raw materials of phase C are added to the mixture b, the fragrance and pH are adjusted, and the mixture is stirred evenly to obtain the essence.

6. The method for preparing the essence according to claim 5, characterized in that: The slow stirring speed in step (1) is 40-60 r / min.

7. The method for preparing the essence according to claim 5, characterized in that: The uniform stirring speed in step (2) and step (3) is 1500-2500 r / min.

8. The method for preparing the essence according to claim 5, characterized in that: In step (4), the pH value is adjusted to 6.0-7.0.

Images