A classification pool cleaning system, method and analyzer

Abstract

The present invention discloses a classification cell cleaning system, method and analyzer. The cleaning system includes a waste discharge module, at least one reagent module and a cleaning module connected to the classification cell; the waste discharge module is used to empty the remaining reaction liquid in the classification cell; the reagent module is used to inject a first preset amount of hemolytic agent into the classification cell; the cleaning module is used to inject a second preset amount of diluent into the classification cell when or after injecting the hemolytic agent, so as to rinse the classification cell and empty it through the waste discharge module after rinsing; the cleaning module is used to inject a third preset amount of diluent into the classification cell after rinsing and emptying, so as to soak the classification cell and empty it through the waste discharge module after soaking; the reagent module is used to aspirate a fourth preset amount of air column after soaking and emptying. The present invention optimizes the timing control in the cleaning process, can improve the cleanliness of the classification cell, reduce the usage amount of the hemolytic agent, and reduce the cost.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and particularly to a classification cell cleaning system, method and analyzer. Background Art

[0002] The white blood cell classification cell is an important component in a sample analyzer. The white blood cell classification cell is mainly used for preparing a sample solution for white blood cell classification. Usually, after the sample solution is prepared and transported to the optical component for detection, the white blood cell classification cell needs to be cleaned to reduce the risk of cross-contamination with the next sample solution to be prepared.

[0003] In current hematology analyzers, a hemolytic agent is used to clean the white blood cell classification cell before white blood cell differential counting. After cleaning, it is emptied and then the corresponding hemolytic agent and blood sample are added for reaction to complete the preparation of the sample solution. Before white blood cell differential counting, if the inner wall of the white blood cell classification cell or the relevant pipeline is not cleaned thoroughly, it will affect the differential counting. In addition, after one measurement, if the inner wall of the white blood cell classification cell is not cleaned thoroughly or the blood cells carried on the pipeline will also affect the measurement accuracy of the next time. When the existing hematology analyzer cleans the white blood cell classification cell, generally, before preparing the sample solution, a hemolytic agent is first used to clean the blood cells carried on the inner wall and pipeline of the white blood cell classification cell. After cleaning, reagents for reaction are added to the classification cell. However, this cleaning method requires a large amount of hemolytic agent consumption, resulting in high costs. Summary of the Invention

[0004] The object of the present invention is to provide a classification cell cleaning system, method and analyzer, aiming to solve the problem of high costs caused by the large amount of hemolytic agent consumed in the cleaning process of the existing classification cell.

[0005] In a first aspect, an embodiment of the present invention provides a classification cell cleaning system, including a waste discharge module, at least one reagent module and a cleaning module. The waste discharge module, reagent module and cleaning module are all connected to the classification cell;

[0006] The waste discharge module is used to empty the remaining reaction liquid in the classification cell;

[0007] The reagent module is used to inject a first preset amount of hemolytic agent into the classification cell;

[0008] The cleaning module is used to inject a second preset amount of diluent into the classification cell when or after injecting the hemolytic agent, so as to rinse the classification cell and empty it through the waste discharge module after rinsing is completed;

[0009] The cleaning module is used to inject a third preset amount of diluent into the classification cell after the flushing and emptying are completed, so as to soak the classification cell and empty it through the waste discharging module after the soaking is completed;

[0010] The reagent module is used to aspirate a fourth preset amount of air column after the soaking and emptying are completed.

[0011] In a second aspect, an embodiment of the present invention provides a method for cleaning a classification cell, which is applied to a classification cell cleaning system. The classification cell cleaning system includes a waste discharging module, at least one reagent module and a cleaning module. The waste discharging module, the reagent module and the cleaning module are all connected to the classification cell. The method for cleaning the classification cell includes:

[0012] Controlling the waste discharging module to empty the remaining reaction liquid in the classification cell;

[0013] Controlling the reagent module to inject a first preset amount of hemolytic agent into the classification cell;

[0014] Controlling the cleaning module to inject a second preset amount of diluent into the classification cell when or after injecting the hemolytic agent, so as to flush the classification cell and empty it through the waste discharging module after the flushing is completed;

[0015] Controlling the cleaning module to inject a third preset amount of diluent into the classification cell after the flushing and emptying are completed, so as to soak the classification cell and empty it through the waste discharging module after the soaking is completed;

[0016] Controlling the reagent module to aspirate a fourth preset amount of air column after the soaking and emptying are completed.

[0017] In a third aspect, an embodiment of the present invention provides an analyzer, including the classification cell cleaning system as described above.

[0018] An embodiment of the present invention discloses a classification cell cleaning system, method and analyzer. The cleaning system includes a classification cell, a waste discharge module, at least one reagent module and a cleaning module, all of which are connected to the classification cell. The waste discharge module is used to empty the remaining reaction liquid in the classification cell. The reagent module is used to inject a first preset amount of hemolytic agent into the classification cell. The cleaning module is used to inject a second preset amount of diluent into the classification cell when or after injecting the hemolytic agent, so as to rinse the classification cell and empty it through the waste discharge module after rinsing. The cleaning module is used to inject a third preset amount of diluent into the classification cell after rinsing and emptying, so as to soak the classification cell and empty it through the waste discharge module after soaking. The reagent module is used to aspirate a fourth preset amount of air column after soaking and emptying. The embodiment of the present invention only needs to consume a small amount of hemolytic agent and cooperate with diluent for rinsing, and then soak and clean with diluent. Compared with the method of completely using hemolytic agent for cleaning, the consumption is less. At the same time, the timing control in the cleaning process is optimized, and the step of aspirating the air column is added to isolate the pollution between the hemolytic agent and the diluent, which can effectively prevent the problem of the hemolytic agent diffusion in the reagent module under long-term static state. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0020] Figure 1 It is a schematic structural diagram of the classification cell cleaning system provided by the embodiment of the present invention;

[0021] Figure 2 It is a schematic flow diagram of the classification cell cleaning method provided by the embodiment of the present invention.

[0022] REFERENCE SIGNS:

[0023] 1, classification cell; 2, first valve; 3, pump body; 4, waste liquid bucket; 5a, syringe; 5b, syringe; 6a, second valve; 6b, second valve; 7a, hemolytic agent storage tank; 7b, hemolytic agent storage tank; 8, third valve; 9, diluent storage tank; 10, positive pressure source; 11, optical module; 12, fourth valve. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part rather than all of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0025] It should be understood that when used in this specification and the appended claims, the terms "comprising" and "including" indicate the presence of the described features, wholes, steps, operations, elements and / or components, but do not preclude the presence or addition of one or more other features, wholes, steps, operations, elements, components and / or their combinations.

[0026] It should also be understood that the terms used in this specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. As used in this specification of the present invention and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include the plural forms.

[0027] It should be further understood that the term "and / or" used in this specification of the present invention and the appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0028] Please refer to Figure 1 , Figure 1 , which is a schematic structural diagram of the classification pool cleaning system provided by the embodiment of the present invention;

[0029] This application is applied to the cleaning of the white blood cell classification pool. To facilitate the understanding of this application, the preparation process of the white blood cell classification and counting sample solution in the classification pool will be introduced first:

[0030] Preparation process of the white blood cell differential count sample solution: Add the first hemolytic agent to the classification cell 1 and mix it with the blood sample for incubation for a period of time, and then add the second hemolytic agent for further incubation (the types of hemolytic agents are set according to actual needs). After the incubation is completed, the obtained sample solution is sent to the optical module 11 for detection, and then the remaining sample solution is drained and the classification cell 1 is cleaned. Among them, since the hemolytic agent reagent tube is generally connected to the lower part of the classification cell 1 and integrally formed with the classification cell 1, in order to improve the mixing effect, the first hemolytic agent and the blood sample are generally added to the classification cell 1 basically at the same time to form a swirl to enhance mixing. Due to the action volume of the on-off valve in the hemolytic agent reagent tube and the relatively fast speed when adding the reagent, after adding the hemolytic agent to the classification cell 1, there will be a certain amount of back suction in the hemolytic agent reagent tube. At this time, the mixed liquid containing the sample may be back-sucked into the hemolytic agent reagent tube. After adding the second hemolytic agent, there will also be a certain amount of back suction in the corresponding reagent pipeline, and the mixed liquid containing the sample will also be sucked into the hemolytic agent reagent tube (that is Figure 1 in the two pipeline parts above the left side of the lower part of the classification cell 1, it is easy to back-suck part of the mixed liquid containing the sample and remain at the pipeline interface). Therefore, although only cleaning the classification cell 1 can clean the classification cell, the residues of the previous test sucked into the hemolytic agent reagent tube cannot be cleaned up, which will affect the subsequent detection. In the prior art, the hemolytic agent is directly used for cleaning. Although the mixed liquid containing the sample that may exist at the pipeline interface can be taken out together, the amount of hemolytic agent consumed for single cleaning in this way is relatively large.

[0031] Furthermore, combined with each component in the classification cell cleaning system of the present application (taking the reagent module including two groups as an example), the sample solution preparation process is introduced in more detail: First, open the first valve 2 and the pump body 3, and convey the waste liquid of the classification cell 1 to the waste liquid bucket 4. While adding the first hemolytic agent to the classification cell 1 through the syringe 5a and the second valve 6a, a quantitative blood sample is injected into the classification cell 1 by an external sampling needle (not shown in the figure, generally injected from the top of the classification cell 1). Then, gas is injected into the classification cell 1 through the positive pressure source 10 and the fourth valve 12 to form bubbles to mix the liquid in the classification cell 1. After incubation for a period of time, the second hemolytic agent is added to the classification cell 1 again through the syringe 5b and the second valve 6b. After the second valve 6a and the second valve 6b are opened, they can be kept open all the time, that is, the syringe is connected to the classification cell 1 and is not closed until the classification cell 1 is completely emptied. After adding the second hemolytic agent, gas is continuously injected into the classification cell 1 for mixing and incubation, so as to complete the preparation of the sample solution. Then, the sample solution is conveyed to the optical module 11 for detection through the third valve 8. Finally, after the remaining sample solution in the classification cell 1 is emptied through the first valve 2 and the pump body 3, the cleaning process can be entered.

[0032] Based on this, please continue to refer to Figure 1, for the cleaning process, the present application provides a classification pool cleaning system, including a classification pool 1, a waste discharge module, at least one reagent module, and a cleaning module. The waste discharge module, the reagent module, and the cleaning module are all connected to the classification pool 1;

[0033] Among them, the waste discharge module includes a first valve 2, a pump body 3, and a waste liquid bucket 4; the classification pool 1, the first valve 2, the pump body 3, and the waste liquid bucket 4 are connected in sequence;

[0034] Among them, the reagent module includes multiple groups. Each group of reagent modules includes a syringe, a second valve, and a hemolytic agent storage tank; in each group of reagent modules, the second valve is connected to the classification pool 1, and the second valve is respectively connected to the syringe and the hemolytic agent storage tank; the second valve is used to control the on-off between the syringe and the classification pool 1, and to control the on-off between the syringe and the hemolytic agent storage tank.

[0035] Among them, the cleaning module includes a third valve 8, a diluent storage tank 9, and a positive pressure source 10; the classification pool 1, the third valve 8, the diluent storage tank 9, and the positive pressure source 10 are connected in sequence.

[0036] Based on the classification pool cleaning system of the present application, the cleaning process is specifically introduced below:

[0037] First, after the sample liquid in the classification pool 1 is transported to the optical module 11, control the first valve 2 to open, and discharge the remaining reaction liquid in the classification pool 1 to the waste liquid bucket 4 through the pump body 3; while discharging, control the syringe 5a to inject a first preset amount of the first hemolytic agent in the hemolytic agent storage tank 7a into the classification pool 1 through the second valve 6a, and control the syringe 5b to inject a first preset amount of the second hemolytic agent in the hemolytic agent storage tank 7b into the classification pool 1 through the second valve 6b; among them, the first preset amount is 5 - 50 ul, preferably 15 ul in this embodiment, and can be specifically set according to the type and specifications of the analyzer, ensuring that the first preset amount of hemolytic agent can completely inject the mixed liquid that may contain the sample at the pipeline interface into the classification pool 1.

[0038] Then, while or after injecting the first hemolytic agent and the second hemolytic agent into the classification pool 1, control the third valve 8 to open, control the positive pressure source 10 to inject a second preset amount of diluent in the diluent storage tank 9 into the classification pool 1 through the third valve 8 for flushing, and while flushing, control the pump body 3 to discharge the flushing waste liquid to the waste liquid bucket 4 through the first valve 2; among them, the second preset amount can be set according to the capacity of the classification pool 1, ensuring effective flushing of the inner wall of the classification pool 1 during flushing.

[0039] Then, after the rinsing and emptying are completed, control the positive pressure source 10 to inject a third preset amount of diluent in the diluent storage tank 9 into the sorting tank 1 through the third valve 8 for soaking. After soaking for a certain period of time, control the pump body 3 to discharge the soaked waste liquid into the waste liquid bucket 4 through the first valve 2, and close the first valve 2 after emptying; the third preset amount of diluent needs to submerge the liquid level of the original sample liquid to ensure better dissolution and cleaning.

[0040] Then, after the soaking and emptying are completed, control the syringe 5a to aspirate a fourth preset amount of air column through the second valve 6a and control the syringe 5b to aspirate a fourth preset amount of air column through the second valve 6b, and close the second valve 6a and the second valve 6b after the aspiration is completed. Aspirating the air column can isolate the contamination between various hemolyzing agents and between the hemolyzing agent and the diluent, and can effectively prevent diffusion caused by long-term stillness (for example, in actual applications, there may be an interval of 15 minutes or more between the detection of the previous sample and the detection of the next sample by the analyzer, which is likely to cause diffusion); the fourth preset amount can be set according to the type and specifications of the analyzer, and in this embodiment, it is preferably 15 ul.

[0041] Finally, after the aspiration is completed and the second valve 6a and the second valve 6b are closed, control the positive pressure source 10 to inject a fifth preset amount of diluent in the diluent storage tank 9 into the sorting tank 1 for secondary soaking, and close the third valve 8; the purpose of the secondary soaking is to keep the sorting tank 1 in a state ready for use at any time, and it also has a certain effect of dissolution and cleaning, and can effectively ensure the continuous use of the two hemolyzing agents in the reagent tube. The time of the secondary soaking is the time difference between two detections. If the sorting tank 1 is directly in an emptied state and waits for the next detection after the first soaking, long-term waiting will cause adverse effects such as volatilization and contamination of the hemolyzing agent.

[0042] Thus, the single cleaning process can be completed. When preparing the sample liquid next time, after emptying the waste liquid of the secondary soaking, the sample liquid preparation can be started, and a cycle is formed in this way. Each cleaning only needs to consume a small amount of hemolyzing agent, cooperate with the diluent for rinsing, and then be soaked and cleaned by the diluent. Compared with the prior art that completely uses the hemolyzing agent for cleaning, the consumption is less. At the same time, the timing control in the cleaning process is optimized, and the step of aspirating the air column is added to isolate the contamination between the hemolyzing agent and the diluent, and can effectively prevent the problem of diffusion of the hemolyzing agent in the reagent module under long-term stillness.

[0043] In some experimental data, for an analyzer of a certain same specification in a single cleaning, the consumption of the method of completely using the hemolyzing agent for cleaning is about 260 ul, and the consumption is relatively large. However, in this application, only 15 ul of each hemolyzing agent is consumed in a single cleaning. Calculated by the two hemolyzing agents in this embodiment, the total consumption is 30 ul, which is only 1 / 9 of the original consumption, greatly reducing the consumption of the hemolyzing agent.

[0044] As shown Figure 2 in Figure 2 Figure 1, it is a schematic flowchart of the cleaning method for the classification cell 1 provided by an embodiment of the present invention;

[0045] As shown Figure 2 in Figure 2, an embodiment of the present invention further provides a cleaning method for the classification cell 1. This method is applied to a classification cell cleaning system, and this method includes steps S201 to S206.

[0046] S201. Control the waste discharge module to empty the remaining reaction liquid in the classification cell 1;

[0047] In this step, first control the first valve 2 to open to connect the pump body 3 and the classification cell 1; then control the pump body 3 to extract the remaining reaction liquid in the classification cell 1, and discharge it to the waste liquid bucket 4 after passing through the first valve 2 and the pump body 3;

[0048] S202. Control the reagent module to inject a first preset amount of hemolytic agent into the classification cell 1;

[0049] In this step, first control each second valve to open and connect the corresponding syringe with the classification cell 1; then control each syringe to inject a first preset amount of the corresponding hemolytic agent into the classification cell 1 through the corresponding second valve.

[0050] S203. Control the cleaning module to inject a second preset amount of diluent into the classification cell 1 when injecting the hemolytic agent or after injecting the hemolytic agent, to rinse the classification cell 1 and empty it through the waste discharge module after the rinsing is completed;

[0051] In this step, first control the third valve 8 to open to connect the diluent storage tank 9 and the classification cell 1; then control the positive pressure source 10 to inject a second preset amount of diluent in the diluent storage tank 9 into the classification cell 1 through the third valve 8 for rinsing; then control the waste discharge module to empty the classification cell 1 after the rinsing is completed.

[0052] S204. Control the cleaning module to inject a third preset amount of diluent into the classification cell 1 after the rinsing and emptying are completed, to soak the classification cell 1 and empty it through the waste discharge module after the soaking is completed;

[0053] In this step, first control the third valve 8 to open to connect the diluent storage tank 9 and the classification cell 1; then control the positive pressure source 10 to inject a third preset amount of diluent in the diluent storage tank 9 into the classification cell 1 through the third valve 8 for soaking; then control the waste discharge module to empty the classification cell 1 after the soaking is completed.

[0054] S205. Control the reagent module to aspirate a fourth preset amount of air column after the soaking and emptying are completed;

[0055] S206. Control the cleaning module to inject a fifth preset amount of diluent into the sorting pool 1 after the first soaking and emptying is completed, so as to perform secondary soaking on the sorting pool 1.

[0056] This method only needs to consume a small amount of hemolytic agent and cooperate with the diluent for flushing, and then be soaked and cleaned by the diluent. Compared with the method of completely using hemolytic agent for cleaning, the consumption is less. At the same time, the timing control in the cleaning process is optimized, and the step of sucking back the air column is added to isolate the pollution between the hemolytic agent and the diluent, which can effectively prevent the problem of hemolytic agent diffusion in the reagent module during long-term static state.

[0057] An embodiment of the present invention also provides an analyzer, which is characterized by including the sorting pool cleaning system as described above.

[0058] Those skilled in the art can clearly understand that for the convenience and conciseness of description, the specific working processes of the above-described method and device can refer to the corresponding processes in the foregoing system embodiments, and will not be repeated here.

[0059] The above is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of various equivalent modifications or substitutions within the technical scope disclosed by the present invention, and these modifications or substitutions should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A classification pool cleaning system, characterized in that: It includes a waste discharging module, at least one reagent module and a cleaning module. The waste discharging module, the reagent module and the cleaning module are all connected to the classification tank; The waste discharging module is used to empty the remaining reaction liquid in the classification tank; The reagent module is used to inject a first preset amount of hemolytic agent into the classification tank; The cleaning module is used to inject a second preset amount of diluent into the classification tank when or after injecting the hemolytic agent, so as to wash the classification tank and empty it through the waste discharging module after the washing is completed; The cleaning module is used to inject a third preset amount of diluent into the classification tank after the washing and emptying are completed, so as to soak the classification tank and empty it through the waste discharging module after the soaking is completed; The reagent module is used to aspirate a fourth preset amount of air column after the soaking and emptying are completed; Wherein, the cleaning module includes a third valve, a fourth valve, a diluent storage tank and a positive pressure source; The classification tank, the third valve, the diluent storage tank and the positive pressure source are connected in sequence; The classification tank, the fourth valve and the positive pressure source are connected in sequence.

2. The classification pool cleaning system according to claim 1, wherein: The waste discharging module includes a first valve, a pump body and a waste liquid bucket; The classification tank, the first valve, the pump body and the waste liquid bucket are connected in sequence.

3. The classification pool cleaning system according to claim 1, characterized in that: The reagent module includes multiple groups. Each group of reagent modules includes a syringe, a second valve and a hemolytic agent storage tank; in each group of reagent modules, the second valve is connected to the classification tank, and the second valve is respectively connected to the syringe and the hemolytic agent storage tank; Wherein, the second valve is used to control the connection and disconnection between the syringe and the classification tank, and to control the connection and disconnection between the syringe and the hemolytic agent storage tank.

4. A classification pool cleaning method, applied to the classification pool cleaning system according to any one of claims 1-3, characterized in that, The classification tank cleaning system includes a waste discharging module, at least one reagent module and a cleaning module. The waste discharging module, the reagent module and the cleaning module are all connected to the classification tank. The classification tank cleaning method includes: Controlling the waste discharging module to empty the remaining reaction liquid in the classification tank; Controlling the reagent module to inject a first preset amount of hemolytic agent into the classification tank; Controlling the cleaning module to inject a second preset amount of diluent into the classification tank when or after injecting the hemolytic agent, so as to wash the classification tank and empty it through the waste discharging module after the washing is completed; Controlling the cleaning module to inject a third preset amount of diluent into the classification tank after the washing and emptying are completed, so as to soak the classification tank and empty it through the waste discharging module after the soaking is completed; Controlling the reagent module to aspirate a fourth preset amount of air column after the soaking and emptying are completed.

5. The classification pool cleaning method according to claim 4, characterized in that The waste discharging module includes a first valve, a pump body and a waste liquid bucket; the classification tank, the first valve, the pump body and the waste liquid bucket are connected in sequence; The controlling the waste discharging module to empty the remaining reaction liquid in the classification tank includes: Controlling the first valve to open to connect the pump body and the classification tank; Controlling the pump body to extract the remaining reaction liquid in the classification tank, and discharging it to the waste liquid bucket after passing through the first valve and the pump body.

6. The classification pool cleaning method according to claim 4, wherein The reagent module includes multiple groups, and each group of reagent modules includes a syringe, a second valve, and a hemolytic agent storage tank; in each group of reagent modules, the second valve is connected to the sorting pool, and the second valve is respectively connected to the syringe and the hemolytic agent storage tank; The control of injecting a first preset amount of hemolytic agent from the reagent module into the sorting pool includes: Controlling each of the second valves to open and connect the corresponding syringe to the sorting pool; Controlling each syringe to inject a first preset amount of the corresponding hemolytic agent into the sorting pool through the corresponding second valve.

7. The classification pool cleaning method according to claim 4, wherein The cleaning module includes a third valve, a diluent storage tank, and a positive pressure source; the sorting pool, the third valve, the diluent storage tank, and the positive pressure source are connected in sequence; The control of injecting a second preset amount of diluent into the sorting pool by the cleaning module for flushing during or after injecting the hemolytic agent and emptying through the waste discharge module after flushing includes: Controlling the third valve to open to connect the diluent storage tank and the sorting pool; Controlling the positive pressure source to inject a second preset amount of diluent in the diluent storage tank into the sorting pool through the third valve for flushing; Controlling the waste discharge module to empty the sorting pool after flushing is completed.

8. The classification pool cleaning method according to claim 7, characterized in that The control of injecting a third preset amount of diluent into the sorting pool by the cleaning module for soaking and emptying through the waste discharge module after soaking is completed includes: Controlling the third valve to open to connect the diluent storage tank and the sorting pool; Controlling the positive pressure source to inject a third preset amount of diluent in the diluent storage tank into the sorting pool through the third valve for soaking; Controlling the waste discharge module to empty the sorting pool after soaking is completed.

9. An analyzer, characterized in that, Including the sorting pool cleaning system according to any one of claims 1 to 3 above.

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