A fluid system of a flow cytometer

Abstract

The present invention discloses a fluid system of a flow cytometer, comprising: a sampling needle, an optical detection system, a sample syringe, a sampling syringe, a fourth reagent, a first reagent, a second reagent, a reaction cell, a waste liquid system, a first isolation chamber, a second isolation chamber, a first bubble sensor, a second bubble sensor, and connectors, valves and pipelines used for connecting each device. On the one hand, the present invention replaces the syringe or metering pump with a valve assembly and replaces the heating structure with an isolation chamber, simplifies the circuit of the heating hemolytic agent, and reduces the cost; on the other hand, the added isolation chamber can utilize buoyancy to reduce the bubbles accidentally sucked in the hemolytic agent in the pipeline, improving the accuracy of the results; in addition, the present invention changes the installation position of the bubble sensor in the traditional fluid system, sets the bubble sensor at the upper pipeline outlet of the isolation chamber, utilizes the principle of the communicating vessel, can accurately detect the content of the remaining reagent, and avoids residual waste.

Description

Technical Field

[0001] The present invention relates to the field of detection, and particularly to a fluid system of a flow cytometer. Background Art

[0002] In a traditional five-category blood cell analyzer, generally, independent syringes or quantitative pump pipelines are used to add two or more kinds of hemolyzing agents. As shown in the following figure, valves are used to control the pipeline direction. The syringe is stretched to generate negative pressure to suck a quantitative hemolyzing agent, and the syringe is pushed to generate positive pressure while opening valve 20(22) to spit the hemolyzing agent into the counting pool. (A Fluid System and Measurement Method of a Flow Cytometer.PDF, "CN108627449A").

[0003] The quantitative pumps or syringes used in the prior art are all components with relatively high costs, which will result in an increase in the total cost. The present invention can completely replace the syringe or quantitative pump with a valve to achieve cost reduction. In addition, fewer liquid path devices are used. Originally, two quantitative pumps or two syringes were required.

[0004] In addition, since an isolation chamber is added in the loop for buffering, sufficient time is given for the sucked reagent to stand still in the isolation chamber. Bubbles are stratified to the upper surface of the liquid in the isolation chamber due to the buoyancy effect, and the reagent added to the reaction pool is from the lower surface, which can ensure that the added reagent has no bubbles.

[0005] In addition, in a traditional flow cytometer, such as the bubble sensor of a five-category blood cell analyzer, is often installed near the reagent bottle, and there is still a long pipeline to the reaction pool. Therefore, when the reagent is insufficient, the bubble sensor receives a signal and alarms, and there is still a large amount of reagent remaining in the pipeline, wasting test times. Summary of the Invention

[0006] Technical Problems to be Solved

[0007] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a fluid system of a flow cytometer, aiming to solve the problems of complex structure, high cost, and inaccurate detection results of the existing flow cytometer.

[0008] The present invention adopts the following technical solutions:

[0009] A fluid system of a flow cytometer, comprising: a sampling needle, an optical detection system, a sample syringe, a sampling syringe, a fourth reagent, a first reagent, a second reagent, a reaction cell, a waste liquid system, a first isolation chamber, a second isolation chamber, a first bubble sensor, a second bubble sensor, and connectors, valves and pipelines used for connecting each device; the sampling needle is connected to the normally open end of a seventh three-way solenoid valve, the normally closed end of the seventh three-way solenoid valve is connected to the optical detection system, and the common end of the seventh three-way solenoid valve is connected to the upper end connector of the sampling syringe; the lower end connector of the sampling syringe is connected to the lower end of a first two-way solenoid valve, the common end of a first three-way solenoid valve is connected to the upper end of the first two-way solenoid valve and the upper end connector of the sample syringe through a three-way joint, the normally closed end of the first three-way solenoid valve is connected to the upper end outlet of the second isolation chamber, the upper lateral offset outlet and the lower lateral offset outlet of the second isolation chamber are connected through a three-way joint, a second bubble sensor is arranged between the upper lateral offset outlet of the second isolation chamber and the three-way joint, the remaining interface of the three-way joint is connected to the common end of a fourth three-way solenoid valve, the normally open end of the fourth three-way solenoid valve is connected to the second reagent, and the normally closed end of the fourth three-way solenoid valve is connected to the reaction cell; the first three-way solenoid valve and the second three-way solenoid valve are arranged in series, and the normally open end of the first three-way solenoid valve is connected to the common end of the second three-way solenoid valve; the normally closed end of the second three-way solenoid valve is connected to the upper end outlet of the first isolation chamber, the upper lateral offset outlet and the lower lateral offset outlet of the first isolation chamber are connected through a three-way joint, a first bubble sensor is arranged between the upper lateral offset outlet of the first isolation chamber and the three-way joint, the remaining interface of the three-way joint is connected to the common end of a third three-way solenoid valve, the normally closed end of the third three-way solenoid valve is connected to the first reagent, and the normally open end of the third three-way solenoid valve is connected to the reaction cell; the second three-way solenoid valve and the sixth three-way solenoid valve are arranged in series, the normally open end of the second three-way solenoid valve is connected to the common end of the sixth three-way solenoid valve, the normally open end of the sixth three-way solenoid valve is connected to the fourth reagent, the normally closed end of the sixth three-way solenoid valve is connected to the reaction cell and the lower end of a fourth two-way solenoid valve through a three-way joint, the upper end of the fourth two-way solenoid valve is connected to the optical detection system, a second two-way solenoid valve is arranged between the optical detection system and the waste liquid system, and a third two-way solenoid valve is arranged between the reaction cell and the waste liquid system.

[0010] Optionally, the fluid system of the above-mentioned flow cytometer further includes a third reagent, a third isolation chamber, a third bubble sensor, an eighth three-way solenoid valve, and a fifth three-way solenoid valve; the fifth three-way solenoid valve is serially arranged between the second three-way solenoid valve and the sixth three-way solenoid valve. The normally open end of the second three-way solenoid valve is connected to the common end of the fifth three-way solenoid valve, the normally open end of the fifth three-way solenoid valve is connected to the common end of the sixth three-way solenoid valve, the normally closed end of the fifth three-way solenoid valve is connected to the upper outlet of the third isolation chamber, the upper lateral outlet and the lower lateral outlet of the third isolation chamber are connected through a three-way joint, and the third bubble sensor is provided between the upper lateral outlet of the third isolation chamber and the three-way joint. The remaining interface of the three-way joint is connected to the common end of the eighth three-way solenoid valve. The normally open end of the eighth three-way solenoid valve is connected to the third reagent, and the normally closed end of the eighth three-way solenoid valve is connected to the reaction cell.

[0011] Optionally, the sample syringe and the sampling syringe share a driving motor.

[0012] Optionally, it further includes a main control board, which is loaded with a preset timing control program for controlling the on-off of all solenoid valves and driving motors and data processing.

[0013] Optionally, each of the isolation chambers is provided with a heating device.

[0014] Optionally, the heating device is a temperature control device with a heating film as the core.

[0015] Optionally, the isolation chamber is further provided with a filter membrane, which is used to prevent the hemolytic agent and gas agitation from generating bubbles.

[0016] Optionally, the bubble sensor is a photoelectric sensor.

[0017] Optionally, the first reagent is a first hemolytic agent, the second reagent is a second hemolytic agent, the third reagent is a third hemolytic agent, and the fourth reagent is a diluent.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0019] The valve assembly is used to replace the syringe or metering pump, and the isolation chamber is used to replace the heating structure, simplifying the circuit for heating the hemolytic agent, mainly reducing the cost; at the same time, the added isolation chamber can utilize buoyancy to reduce the accidental absorption of bubbles hidden in the hemolytic agent in the pipeline, improving the accuracy of the results; finally, when the amount of the hemolytic agent is insufficient, there is a large waste of the hemolytic agent in the pipeline due to the replacement of the hemolytic agent. Therefore, the installation position of the replaced bubble sensor in the present invention can save the hemolytic agent. Description of the Drawings

[0020] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other accompanying drawings can be obtained based on these drawings.

[0021] Figure 1 is a block diagram of a flow cytometer fluid system of the prior art;

[0022] Figure 2 is a block diagram of a flow cytometer fluid system provided in Embodiment 1 of the present invention;

[0023] Figure 3 is a block diagram of a flow cytometer fluid system provided in Embodiment 2 of the present invention.

[0024] The reference numerals in the figure respectively represent: SV1 the first three-way solenoid valve, SV2 the second three-way solenoid valve, SV3 the third three-way solenoid valve, SV4 the fourth three-way solenoid valve, SV5 the fifth three-way solenoid valve, SV6 the sixth three-way solenoid valve, SV7 the seventh three-way solenoid valve, SV8 the eighth three-way solenoid valve, SV11 the first two-way solenoid valve, SV12 the second two-way solenoid valve, SV13 the third two-way solenoid valve, SV14 the fourth two-way solenoid valve. Detailed Embodiments

[0025] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention.

[0026] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0027] Embodiment 1

[0028] This embodiment includes two reagents, such as two hemolysins, such as Figure 2As described above, a fluid system of a flow cytometer provided by the present invention includes: a sampling needle, an optical detection system, a sample syringe, a sampling syringe, a fourth reagent, a first reagent, a second reagent, a reaction cell, a waste liquid system, a first isolation chamber, a second isolation chamber, a first bubble sensor, a second bubble sensor, and connectors, valves, and pipelines used for connecting each device; the sampling needle is connected to the normally open end of a seventh three-way solenoid valve, the normally closed end of the seventh three-way solenoid valve is connected to the optical detection system, and the common end of the seventh three-way solenoid valve is connected to the upper connector of the sampling syringe; the lower connector of the sampling syringe is connected to the lower end of a first two-way solenoid valve, the common end of a first three-way solenoid valve is connected to the upper end of the first two-way solenoid valve and the upper connector of the sample syringe through a three-way connector, the normally closed end of the first three-way solenoid valve is connected to the upper outlet of the second isolation chamber, the upper lateral outlet and the lower lateral outlet of the second isolation chamber are connected through a three-way connector, a second bubble sensor is provided between the upper lateral outlet of the second isolation chamber and the three-way connector, the remaining interface of the three-way connector is connected to the common end of a fourth three-way solenoid valve, the normally open end of the fourth three-way solenoid valve is connected to the second reagent, and the normally closed end of the fourth three-way solenoid valve is connected to the reaction cell; the first three-way solenoid valve and the second three-way solenoid valve are arranged in series, and the normally open end of the first three-way solenoid valve is connected to the common end of the second three-way solenoid valve; the normally closed end of the second three-way solenoid valve is connected to the upper outlet of the first isolation chamber, the upper lateral outlet and the lower lateral outlet of the first isolation chamber are connected through a three-way connector, a first bubble sensor is provided between the upper lateral outlet of the first isolation chamber and the three-way connector, the remaining interface of the three-way connector is connected to the common end of a third three-way solenoid valve, the normally closed end of the third three-way solenoid valve is connected to the first reagent, and the normally open end of the third three-way solenoid valve is connected to the reaction cell; the second three-way solenoid valve and the sixth three-way solenoid valve are arranged in series, the normally open end of the second three-way solenoid valve is connected to the common end of the sixth three-way solenoid valve, the normally open end of the sixth three-way solenoid valve is connected to the fourth reagent, the normally closed end of the sixth three-way solenoid valve is connected to the reaction cell and the lower end of a fourth two-way solenoid valve through a three-way connector, the upper end of the fourth two-way solenoid valve is connected to the optical detection system, a second two-way solenoid valve is provided between the optical detection system and the waste liquid system, and a third two-way solenoid valve is provided between the reaction cell and the waste liquid system. The sample syringe and the sampling syringe share a driving motor for pushing, the volume of the sample syringe is 10 ml, and the volume of the sampling syringe is 250 μl. The fluid system further includes a main control board, which carries a preset timing control program for controlling the on-off of all solenoid valves and the driving motor and data processing. Heating devices are provided in both isolation chambers, and the heating devices are temperature control devices with heating films as the core. Filter membranes are also provided in the isolation chambers, and the filter membranes are used to prevent the generation of bubbles caused by the agitation of hemolysate and gas. The bubble sensors are photoelectric sensors.The first reagent is a first hemolytic agent, the second reagent is a second hemolytic agent, the first reagent is a first hemolytic agent, the second reagent is a second hemolytic agent, and the fourth reagent is a diluent.

[0029] Refer to the attached drawing of the prior art Figure 1 , taking a five-class hematology analyzer as an example, a fluid system of a flow cytometer provided by the present invention has other parts unchanged. The improved pipeline part consists of 4 three-way solenoid valves, 2 isolation chambers, 2 bubble sensors and several hoses. The control of all solenoid valves and sensors and other electrical components is automatically controlled by the main control board to turn on and off the power and collect sensor signals through the programmed timing. The isolation chamber is attached with a temperature control device with a heating film as the core, which is used to heat the reagent to the target temperature. In addition, there is a filter membrane in the isolation chamber to prevent the generation of bubbles caused by the agitation of the hemolytic agent and gas. The improved pipeline is improved from the outlet pipeline of the traditional sheath fluid and sample double syringe. A three-way solenoid valve SV1 is added at the outlet of the 10 ml syringe. The normally closed port is connected to the original pipeline, and the normally open port is connected to the upper outlet of the isolation chamber 2. The upper lateral outlet and the lower outlet of the isolation chamber are connected through a three-way T-shaped joint. A bubble sensor (photoelectric sensor) is added between the upper lateral outlet and the three-way joint. The remaining interface of the three-way joint is connected to the common end of the three-way solenoid valve SV4. The normally closed end outlet is connected to the bottle of reagent 1, and the normally open end is connected to the inlet of the reaction pool. Similarly, a three-way solenoid valve SV2 is externally connected to the main pipeline of the 10 ml syringe. Its normally closed end is connected to the upper part of the isolation chamber 1. The two lateral outlets are connected to a three-way joint. A bubble sensor 1 is installed between the upper lateral interface and the three-way joint. The remaining port of the three-way joint is connected to the common end of the three-way solenoid valve SV3. The normally closed end is connected to the reaction pool. The reason why the normally open end and the normally closed end of the three-way solenoid valve SV4 are different from those of the other two is that since reagent 1 and reagent 2 use the same pressure source, it is impossible for the two to aspirate or add samples at the same time. Therefore, the on-off of the solenoid valves is staggered for connection.

[0030] In this embodiment, the working process of the flow cytometer is as follows: Taking the addition of the first reagent as an example, the three-way solenoid valve SV2 is powered on, and the double syringe is pulled out a certain distance. At this time, since the pipeline between the solenoid valve SV2 and the first isolation chamber is filled with gas, the gas is subjected to a pulling force to establish a negative pressure, and the negative pressure pulls the hemolytic agent in the first reagent into the first isolation chamber. Due to the presence of gas in the pipeline, the rigidity decreases. Therefore, it is necessary to compensate for the number of steps of the syringe pull. At the same time, since the hemolytic agent is prone to form bubbles, the pulling speed of the syringe should not be too fast, and it should be kept as uniform as possible. And with the protection of the filter membrane, the bubbles or liquid in the isolation chamber will not backflow into the syringe. After the first reagent enters the first isolation chamber, it can be heated inside and left standing until it is time to add the first reagent. During the standing process, even if there are bubbles mixed in the first reagent, they will rise to the liquid surface position under the action of buoyancy, which can greatly reduce the bubbles of the hemolytic agent added to the reaction pool when adding. The action of adding the first reagent is: the three-way solenoid valves SV2 and SV3 are powered on, and the double syringe is pushed back a certain number of steps. At this time, a positive pressure is generated in the pipeline, and the first reagent is directly added to the reaction pool. Similarly, the suction and discharge of the second reagent have the same working process and principle. When the amount of hemolytic agent in the reagent bottle is insufficient, such as the first reagent, the liquid volume inhaled by the double syringe into the first isolation chamber is insufficient, resulting in air above the first isolation chamber. At this time, since the pipeline detected by the first bubble sensor is connected to the first isolation chamber and has the same pressure, according to the principle of the same liquid level in the communicating vessel, there is no reagent in the upper part of the first isolation chamber. The corresponding first bubble sensor at the same height detects bubbles or no liquid, the light intensity changes, and a signal is transmitted to the main control board. The main control board processes the fault data, reports the insufficient reagent margin to the software, stops counting, and the counting function can only be restored after replacing the reagent.

[0031] An experiment was conducted using a five-classification whole blood analyzer. The initial platforms were the same, a control group was set up, and the data of the control group were recorded.

[0032] Control group: In the experiment, a self-made double syringe (10ml + 250ul) was connected to the main pipeline, and the main pipeline was directly connected to the fourth reagent bottle. The syringe was controlled to fill the liquid in the main pipeline. The hemolytic agent branch was composed of two metering pumps and two SMC three-way solenoid valves. The hemolytic agent in the DIFF hemolytic agent pipeline flowed through the heating module and was heated by winding the pipe. The reagent bottle was shaken before sucking the hemolytic agent to generate bubbles during the reagent sucking process.

[0033] Experimental group: The same syringe and main pipeline as in the control group were used. The pipeline and metering pump for the hemolytic agent were cancelled, and two SMC three-way solenoid valves were connected in series in the main pipeline. Pipelines were led out from the two normally closed ends to the isolation chamber and the three-way valve respectively. The specific connection order and direction were the same as those of the liquid path Figure 1 consistent. The outer circle of the isolation chamber was surrounded by a heating film and heat insulation cotton. The reagent bottle was shaken before sucking the hemolytic agent to generate bubbles during the reagent sucking process.

[0034] The experimental results are as follows:

[0035] The liquid addition speed of the experimental group is faster than that of the control group. For the DIFF hemolytic agent, it is 0.5 seconds faster, and for the LH hemolytic agent, it is 0.15 seconds faster. Since the hemolytic agent and the main liquid path can be added with liquid in parallel in the normal whole blood time sequence, the liquid addition speed has no advantage in the measurement speed. However, in the time sequence of replacing the hemolytic agent, the experimental group of the DIFF hemolytic agent is 11 seconds faster than the control group, and the LH hemolytic agent is 20 seconds faster. The time sequence operation is to empty the pipeline and perfusion the hemolytic agent. The heating speed of the experimental group is 17 seconds slower than that of the control group, but due to the long execution time of the startup process and the minimum measurement time of 1 minute per sample, there is no impact. In terms of cost, two metering pumps are saved, and the sampling cost of the heating module is reduced. In the experiment of bubbles, the inhaled bubbles are successfully separated above the isolation chamber in the experimental group, while the control group still adds the bubbles into the reaction pool. When reporting that the reagent remaining amount is insufficient, the experimental group uses the hemolytic agent more fully than the control group, and the specific amount is also related to the pipe length. The volume of the hemolytic agent saved per pipeline in this experiment is about 2.4 ml.

[0036] Example 2

[0037] This embodiment includes three reagents, such as three hemolytic agents, as Figure 3 shown, and other parts are the same as Figure 2 those in the previous one. The difference is that the fluid system further includes a third reagent, a third isolation chamber, a third bubble sensor, an eighth three-way solenoid valve, and a fifth three-way solenoid valve; the fifth three-way solenoid valve is serially arranged between the second three-way solenoid valve and the sixth three-way solenoid valve. The normally open end of the second three-way solenoid valve is connected to the common end of the fifth three-way solenoid valve, the normally open end of the fifth three-way solenoid valve is connected to the common end of the sixth three-way solenoid valve, the normally closed end of the fifth three-way solenoid valve is connected to the upper end outlet of the third isolation chamber, the upper lateral outlet and the lower lateral outlet of the third isolation chamber are connected through a three-way joint, the third bubble sensor is provided between the upper lateral outlet of the third isolation chamber and the three-way joint, the remaining interface of the three-way joint is connected to the common end of the eighth three-way solenoid valve, the normally open end of the eighth three-way solenoid valve is connected to the third reagent, and the normally closed end of the eighth three-way solenoid valve is connected to the reaction pool. The third reagent is the third hemolytic agent.

[0038] It should be noted that: the structure of the fluid system of the present invention includes but is not limited to the combination of two or three reagents, and can be extended to more reagents. This pipeline design is applicable to all reagents that need to be added separately. According to specific needs, the heating device can be replaced with a Peltier cooler, and the bubble detection device of the reagent can be replaced with other sensors that can detect changes in the liquid state.

[0039] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A fluid system of a flow cytometer, characterized in that, Comprising: A sampling needle, an optical detection system, a sample syringe, a sampling syringe, a fourth reagent, a first reagent, a second reagent, a reaction cell, a waste liquid system, a first isolation chamber, a second isolation chamber, a first bubble sensor, a second bubble sensor, and connectors, valves, and pipelines used for connecting each device; the sampling needle is connected to the normally open end of a seventh three-way solenoid valve, the normally closed end of the seventh three-way solenoid valve is connected to the optical detection system, and the common end of the seventh three-way solenoid valve is connected to the upper connector of the sampling syringe; the lower connector of the sampling syringe is connected to the lower end of a first two-way solenoid valve, the common end of a first three-way solenoid valve is connected to the upper end of the first two-way solenoid valve and the upper connector of the sample syringe through a three-way joint, the normally closed end of the first three-way solenoid valve is connected to the upper outlet of the second isolation chamber, the laterally upper outlet and the laterally lower outlet of the second isolation chamber are connected through a three-way joint, a second bubble sensor is provided between the laterally upper outlet of the second isolation chamber and the three-way joint, the remaining interface of the three-way joint is connected to the common end of a fourth three-way solenoid valve, the normally open end of the fourth three-way solenoid valve is connected to the second reagent, and the normally closed end of the fourth three-way solenoid valve is connected to the reaction cell; the first three-way solenoid valve and a second three-way solenoid valve are arranged in series, and the normally open end of the first three-way solenoid valve is connected to the common end of the second three-way solenoid valve; the normally closed end of the second three-way solenoid valve is connected to the upper outlet of the first isolation chamber, the laterally upper outlet and the laterally lower outlet of the first isolation chamber are connected through a three-way joint, a first bubble sensor is provided between the laterally upper outlet of the first isolation chamber and the three-way joint, the remaining interface of the three-way joint is connected to the common end of a third three-way solenoid valve, the normally closed end of the third three-way solenoid valve is connected to the first reagent, and the normally open end of the third three-way solenoid valve is connected to the reaction cell; the second three-way solenoid valve and a sixth three-way solenoid valve are arranged in series, the normally open end of the second three-way solenoid valve is connected to the common end of the sixth three-way solenoid valve, the normally open end of the sixth three-way solenoid valve is connected to the fourth reagent, the normally closed end of the sixth three-way solenoid valve is connected to the reaction cell and the lower end of a fourth two-way solenoid valve through a three-way joint, the upper end of the fourth two-way solenoid valve is connected to the optical detection system, a second two-way solenoid valve is provided between the optical detection system and the waste liquid system, and a third two-way solenoid valve is provided between the reaction cell and the waste liquid system.

2. The fluid system of the flow cytometer according to claim 1, characterized in that, It further includes a third reagent, a third isolation chamber, a third bubble sensor, an eighth three-way solenoid valve, and a fifth three-way solenoid valve; the fifth three-way solenoid valve is serially arranged between the second three-way solenoid valve and the sixth three-way solenoid valve, the normal open end of the second three-way solenoid valve is connected to the common end of the fifth three-way solenoid valve, the normal open end of the fifth three-way solenoid valve is connected to the common end of the sixth three-way solenoid valve, the normally closed end of the fifth three-way solenoid valve is connected to the upper outlet of the third isolation chamber, the upper side and lower side outlets of the third isolation chamber are connected through a three-way joint, the third bubble sensor is provided between the upper side outlet of the third isolation chamber and the three-way joint, the remaining interface of the three-way joint is connected to the common end of the eighth three-way solenoid valve, the normal open end of the eighth three-way solenoid valve is connected to the third reagent, and the normally closed end of the eighth three-way solenoid valve is connected to the reaction pool.

3. The fluid system of the flow cytometer according to claim 1 or 2, characterized in that, The sample syringe and the sampling syringe share a driving motor.

4. The fluid system of the flow cytometer according to claim 3, characterized in that It further includes a main control board, which carries a preset timing control program for controlling the power on and off of all solenoid valves and the driving motor as well as data processing.

5. The fluid system of the flow cytometer according to claim 1 or 2, characterized in that, Each of the isolation chambers is provided with a heating device.

6. The fluid system of the flow cytometer according to claim 5, characterized in that: The heating device is a temperature control device with a heating film as the core.

7. The fluid system of the flow cytometer according to claim 1 or 2, characterized in that, Each of the isolation chambers is provided with a filter membrane, which is used to prevent the hemolytic agent and gas agitation from generating bubbles.

8. The fluid system of the flow cytometer according to claim 1 or 2, characterized in that, Each of the bubble sensors is an optoelectronic sensor.

9. The fluid system of the flow cytometer according to claim 1, characterized in that, The first reagent is a first hemolytic agent, the second reagent is a second hemolytic agent, and the fourth reagent is a diluent.

10. The fluid system of the flow cytometer according to claim 2, wherein, The third reagent is a third hemolytic agent.

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