Stress-resistant dermatophyte and its application

Abstract

The present invention discloses stress-resistant Geodermatophilus resistens and its application. The present invention provides stress-resistant Geodermatophilus resistens BI304, which has a registration number of CGMCC No. 25921 in the General Microbiological Center of the China Microbiological Culture Collection Administration. The strain BI304 of the present invention has alkaline phosphatase activity and is a functional new species of Geodermatophilus resistens, which has broad application prospects in garbage disposal, soil remediation, desert control, soil quality improvement, etc.

Description

Technical Field

[0001] The invention relates to the field of microorganisms, and in particular to stress-resistant geodermatophilic bacteria and applications thereof. Background Art

[0002] In the actinomycete systematic classification framework based on 16S rRNA gene sequence analysis, Geodermatophilaceae actinomycetes are a family in the domain of bacteria, phylum Actinobacteria, class Actinobacteria, subclass Actinobacteridae, and order Frankiales, and Geodermatophilaceae is its typical genus. Geodermatophilaceae is a relatively young taxonomic unit among Actinomycetes (Zhi XY, Li WJ, Stackebrandt E. An update of the structure and 16S rRNA genesequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 2009; 59: 589–608; Montero-Calasanz MDC, Meier-Kolthoff JP, Zhang DF, Yaramis A, Rohde M et al. Genome-scale data call for a taxonomic rearrangement of Geodermatophilaceae. Front Microbiol 2017; 8: 2501.). In 1968, Luedemann established the genus Geodermatophilus (Luedemann GM. Geodermatophilus, a new genus of the Dermatophilaceae (Actinomycetales). J Bacteriol 1968; 96: 1848-1858.). In 1980, Skerman et al. formally included it in the Approved List of Bacterial Names (Skerman VBD, McGowan V, Sneath PHA. Approved lists of bacterial names. Int J Syst Bacteriol 1980; 30: 225-420.).Difficulties in pure culture technology of microorganisms once hindered the progress of research on Geodermatophilus and other related actinomycetes. For a long time, Geodermatophilus only included the only type species and four invalid subspecies, namely G. obscures and G. obscurus subsp.amargosae, G. obscurus subsp.utahensis, G. obscurus subsp.dictyosporu, and G. obscurus subsp.everesti. In 1996, Normand proposed to establish the Geodermatophilaceae, but it has not been recognized. It was not until 2006 that Normand combined the common characteristics of three genera, including Geodermatophilus, Blastococcus, and Modestobacter, and comprehensively summarized the typical characteristics of Geodermatophilaceae, finally making Geodermatophilaceae officially included in a family of actinomycetes. In recent years, the research on Geodermatophilus actinomycetes has made good progress. To date, Geodermatophilus has become the largest clade in the family, covering 24 species (https: / / lpsn.dsmz.de / genus / geodermatophilus).In recent years, with the improvement of actinomycete isolation and cultivation research methods, new members have been continuously isolated from the Sahara Desert (Montero-Calasanz Mdel C, Goker M, Potter G, Rohde M, Sproer C, Schumann P, Gorbushina AA, Klenk HP. Geodermatophilus africanus sp. nov., a halotolerant actinomycete isolated from Saharan desert sand. Antonie Van Leeuwenhoek 2013; 104: 207-216.), dry-hot valleys (Nie GX, Ming H, Li S, Zhou EM, Cheng J, Yu TT, Zhang J, Feng HG, Tang SK, Li WJ. Geodermatophilus nigrescens sp. nov., isolated from a dry-hot valley. Antonie Van Leeuwenhoek 2012; 101: 811-817.), and plant rhizosphere soils (Zhang YQ, Chen J, Liu HY, Zhang YQ, Li WJ, Yu LY. Geodermatophilus ruber sp.nov., isolated from rhizosphere soil of a medicinal plant. Int JSyst Evol Microbiol 2011; 61: 190-193.), grassland soil, lake sediment surface, dolomite marble, deep-sea sediments, polar soils, plants, and biological soil crusts, most of which are from desert habitats (https: / / lpsn.dsmz.de / genus / geodermatophilus). Geodermatophilus ruber belongs to the genus Actinomycetes, and its cells are spherical or rod-shaped; some strains can form motile spores; the cell wall is relatively thick; the colony is dark orange to black; and the nutrient utilization spectrum is wide. These phenotypic characteristics are conducive to the survival and spread of strains in environments with large fluctuations in ionizing radiation, drastic changes in temperature, humidity, and pH, and extremely poor nutrition.The cell wall peptidoglycan of this genus contains meso-DAP, the dominant respiratory quinone is MK-9 (H4), and polar lipids mainly include diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylcholine (PC) and some phospholipids containing sugar groups (GPL). The strong tolerance of Geodermataceae actinomycetes to harsh environments such as drought, high temperature, ion radiation, high salt, metal ions and low nutrient supply makes it a microbial model for studying the mechanism of biological stress resistance; under the pressure of various harsh extreme environments, the evolution of metabolic pathways of Geodermataceae actinomycetes has broadened their ability to utilize a variety of carbon source substrates, thereby further enhancing the ability of this type of microorganisms to improve environments such as rocks that are not conducive to the reproduction of life (Sun Hongmin et al. Research progress of Geodermataceae actinomycetes. Journal of Microbiology. 2015, 55(12): 1521-1529.). Summary of the invention

[0003] The purpose of the present invention is to provide a new species of the genus Geodermatophilus.

[0004] In a first aspect, the present invention claims a new species of Geoderma.

[0005] The new species of Geodermatophilus claimed to be protected by the present invention is specifically Geodermatophilus resistens BI304, and its registration number in the General Microbiological Center of China National Microbiological Culture Collection is CGMCC No. 25921.

[0006] The Geodermatophilus resistens BI304 is an aerobic, mesophilic microorganism, has no aerial hyphae, is rod-shaped to irregularly rod-shaped, some cells can swim, and the cells have terminal flagella, and forms black to brown colonies on a PYG medium with a colony diameter of 0.9-1.5 mm.

[0007] In a second aspect, the invention claims a culture.

[0008] The culture claimed in the present invention is a substance obtained by culturing the Geodermatophilus resistens BI304 described in the first aspect in a bacterial culture medium.

[0009] In the above culture, the substances include the Geodermatophilus resistens BI304 (the bacteria itself) and metabolites of the Geodermatophilus resistens BI304.

[0010] In the above culture, the bacterial culture medium may be a solid culture medium or a liquid culture medium.

[0011] The term "culture" refers to a general term for liquid or solid culture media that have a microbial population after artificial inoculation and culture. That is, the product obtained by growing and / or amplifying microorganisms, which can be a biologically pure culture of microorganisms, or it can contain a certain amount of culture medium, metabolites or other components produced during the culture process. The term "culture" also includes a subculture obtained by subculturing microorganisms, which can be a culture of a certain generation or a mixture of several generations.

[0012] In a specific embodiment of the present invention, the bacterial culture medium is PYG culture medium.

[0013] In a third aspect, the present invention claims a metabolite.

[0014] The metabolite claimed in the present invention is the metabolite of Geodermatophilus resistens BI304 described in the first aspect above.

[0015] The term "metabolite" refers to the primary metabolites and / or secondary metabolites produced during the metabolism of microorganisms. Primary metabolism refers to the process in which microorganisms absorb various nutrients from the outside world and generate substances and energy to maintain life activities through catabolism and anabolism. The products of primary metabolism are primary metabolites, such as monosaccharides or monosaccharide derivatives, nucleotides, vitamins, amino acids, fatty acids and other monomers, as well as various macromolecular polymers composed of them, such as proteins, nucleic acids, polysaccharides, lipids, etc. Secondary metabolism refers to the process in which microorganisms use primary metabolites as precursors to synthesize some substances that have no clear function in the life activities of microorganisms during a certain growth period. The products of secondary metabolism are secondary metabolites, most of which are compounds with relatively complex molecular structures. According to their functions, they can be divided into types such as antibiotics, hormones, alkaloids, toxins, etc.

[0016] In a fourth aspect, the present invention claims protection for a bacterial agent.

[0017] The bacterial agent claimed in the present invention contains the Geodermatophilus resistens BI304 described in the first aspect above, the culture described in the second aspect above, and / or the metabolites described in the third aspect above.

[0018] The bacterial agent is a bacterial agent that degrades organic phosphorus.

[0019] In the above-mentioned microbial agent, in addition to the active ingredient, the microbial agent also contains a carrier. The carrier may be a carrier commonly used in the field of pesticides and biologically inert. The carrier may be a solid carrier or a liquid carrier; the solid carrier may be a mineral material, a plant material or a polymer compound; the mineral material may be at least one of clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica and diatomaceous earth; the plant material may be at least one of corn flour, soybean flour and starch; the polymer compound may be polyvinyl alcohol and / or polyglycol; the liquid carrier may be an organic solvent, vegetable oil, mineral oil or water; the organic solvent may be decane and / or dodecane.

[0020] The above-mentioned bacterial agents may be in various dosage forms, such as liquid, emulsion, suspension, powder, granule, wettable powder or water dispersible granule.

[0021] As required, a surfactant (such as Tween 20, Tween 80, etc.), a binder, a stabilizer (such as an antioxidant), a pH adjuster, etc. may also be added to the bacterial agent.

[0022] In a fifth aspect, the present invention claims the use of the Geodermatophilus resistens BI304 described in the first aspect above, or the culture described in the second aspect above, or the metabolite described in the third aspect above, or the bacterial agent described in the fourth aspect above in any of the following:

[0023] (A1) Degradation of organic phosphorus;

[0024] (A2) preparing products for degrading organic phosphorus;

[0025] (A3) Preparing a product having alkaline phosphatase activity.

[0026] In a sixth aspect, the present invention claims the use of the Geodermatophilus resistens BI304 described in the first aspect above, or the culture described in the second aspect above, or the metabolite described in the third aspect above, or the bacterial agent described in the fourth aspect above in any of the following:

[0027] (B1) Waste disposal;

[0028] (B2) Soil remediation;

[0029] (B3) Desertification control;

[0030] (B4) Soil improvement.

[0031] In a seventh aspect, the present invention claims a product for degrading organic phosphorus.

[0032] The product for degrading organic phosphorus claimed in the present invention has the active ingredient of Geodermatophilus resistens BI304 described in the first aspect above, or the culture described in the second aspect above, or the metabolite described in the third aspect above, or the bacterial agent described in the fourth aspect above.

[0033] In an eighth aspect, the present invention claims a product having alkaline phosphatase activity.

[0034] The product with alkaline phosphatase activity claimed in the present invention has as its active ingredient the Geodermatophilus resistens BI304 described in the first aspect above, or the culture described in the second aspect above, or the metabolite described in the third aspect above, or the bacterial agent described in the fourth aspect above.

[0035] In a ninth aspect, the present invention claims a method for degrading organic phosphorus.

[0036] The method for degrading organic phosphorus claimed in the present invention may include the following steps: treating the sample to be treated with the Geodermatophilus resistens BI304 described in the first aspect above, or the culture described in the second aspect above, or the metabolite described in the third aspect above, or the bacterial agent described in the fourth aspect above.

[0037] Since anti-UV genes (uvrA, uvrB, uvrC), DNA damage repair related genes (recO, recQ), carbon starvation tolerance related genes (carbon monoxide dehydrogenase, coxS2) related genes, etc. were detected from the genome of the Geodermatophilus resistens BI304 (Appendix 1). These genes lay the genetic material foundation for the Geodermatophilus resistens BI304 to perform pioneer biological functions in harsh habitats such as deserts. Therefore, the applications or methods involved in the above aspects can all be applications or methods in adverse environments.

[0038] In the tenth aspect, the present invention claims protection for the use of Geodermatophilus resistens BI304 described in the first aspect above in the preparation of the culture described in the second aspect above or the metabolite described in the third aspect above or the bacterial agent described in the fourth aspect above.

[0039] Experiments have shown that the strain BI304 of the present invention represents a new species of the genus Geodermatophilus, named Geodermatophilus resistens. At the same time, through the detection of phosphorus solubilization activity, it is also proved that the strain of the present invention has alkaline phosphatase activity, and is a functional new species of Geodermatophilus, which has broad application prospects in garbage disposal, soil remediation, desert control, soil quality improvement, etc.

[0040] Collection Instructions

[0041] Classification name: Geodermatophilus resistens;

[0042] Reference biological materials: BI304;

[0043] Depository: General Microbiology Center, China Microbiological Culture Collection Administration;

[0044] Abbreviation of depository institution: CGMCC;

[0045] Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing;

[0046] Deposit date: October 17, 2022;

[0047] The registration number of the Collection Center is: CGMCC No.25921. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 The colony morphology of strain BI304 cultured on PYG medium at 28°C for 48 hours. The left picture is a collective photo of colonies, showing that multiple colonies have grown together to form a bacterial lawn; the right picture shows the morphology of multiple single colonies obtained by streaking culture.

[0049] Figure 2 This is the analysis result of polar lipid components in strain BI304 cells.

[0050] Figure 3 A phylogenetic tree was constructed based on the 16S rRNA gene sequences of strain BI304 and all the effectively described species of the genus Geoderma to show the phylogenetic status of strain BI304. The phylogenetic tree used Frankia alni ACN14A (GU296534) as the outgroup. DETAILED DESCRIPTION

[0051] The present invention is further described in detail below in conjunction with specific embodiments, and the examples provided are only for illustrating the present invention, rather than for limiting the scope of the present invention. The examples provided below can be used as a guide for further improvements by those of ordinary skill in the art, and do not constitute a limitation of the present invention in any way.

[0052] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.

[0053] Example 1. Isolation and identification of Geodermatophilus resistens BI304 I. Isolation and screening of strain BI304

[0054] Bacteria isolation medium: The solvent is water, and the solutes and concentrations are as follows: cellobiose 2g / L, yeast extract 5g / L, CaCO3 2g / L, K2HPO4 1g / L, MgSO4·7H2O 0.5g / L, complex vitamins (0.1%, w / v), complex trace salt solution (0.1%, v / v), agar 20g / L, pH 7.2. The complex vitamin formula is: vitamin B1 15%, vitamin B2 15%, vitamin B3 15%, vitamin B6 15%, phenylalanine 15%, biotin 15%, alanine 10%, each % is w / v, specifically g / 100mL. The complex vitamin preparation method is to grind the above substances into fine powder and mix them evenly. The formula of the composite trace salt solution is: FeSO4·7H2O 0.2g, MnCl2·2H2O 0.1g, ZnSO4·7H2O 0.1g, dissolved in sterile water and made up to 100mL.

[0055] The soil samples used for strain isolation were collected from the rhizosphere soil of Stellera chamaejasme plants in Yunnan Alpine Botanical Garden. The fresh soil samples were air-dried at room temperature for 2 weeks and then dry-heated at 120°C for 15 minutes. 2 g of dry-heated soil was added to 18 mL of sterile saline and placed in a shaker at 28°C for 40 minutes at a speed of 200 r / min to fully suspend the soil particles. Gradient dilution was used to prepare 10 -4 Dilution of soil sample suspension.

[0056] Isolation and purification of strains: Take 0.2 mL and apply it on the separation medium plate, invert and culture at 28°C for 6 weeks. Observe the colony status on the separation medium every week, pick out the single colonies of actinomycetes with differences according to the colony characteristics (shape, color, size, surface gloss, etc.) and place them on the PYG medium plate (medium ingredients: peptone 3g / L, yeast extract powder 5g / L, glycerol 10g / L, betaine 1.25g / L, sodium pyruvate 1.25g / L, agar 15g / L, pH 7.2), and purify the culture by the quarter streak method. The obtained pure strains are stored in liquid nitrogen and frozen at -80°C with 20% (v / v) glycerol as a protective agent.

[0057] During this process, strain BI304 was isolated and purified.

[0058] 2. Identification of strain BI304

[0059] 1. Cell morphology observation and physiological and biochemical characteristics detection of strain BI304

[0060] After strain BI304 was cultured on PYG medium at 28°C for 48h, cell morphology was observed using a transmission electron microscope (JEM-1400, JEDL, Japan Electronics). The growth temperature detection range of strain BI304 was 4, 10, 28, 30, 32, 37, 40, 42 and 45°C; the growth salt concentration (NaCl) detection range was 0, 1, 3, 5, 7, 10% (g / 100mL); the growth pH detection range was 8 (4, 5, 6, 7, 8, 9, 10, 11) gradients between pH 4-11. The physiological and biochemical characteristics of the strain were detected using detection kits such as API 50CH, APIZYM, and BiOLOG GEN III carbon source. Other physiological characteristics of strains, including Gram staining properties, oxygen requirement, catalase activity, oxidase activity, gelatin hydrolysis activity, starch hydrolysis activity and cellulose hydrolysis activity, were mainly determined with reference to the Manual of Systematic Identification of Actinomycetes (Xu LH (2007). Actinomycete systematics: principles, methods and practices. Beijing: Science Press, 93-108.).

[0061] The identification results showed that strain BI304 was an aerobic, mesophilic microorganism. It had no aerial hyphae, was rod-shaped to irregularly rod-shaped, some cells were motile, and the cells had terminal flagella. It formed black to brown colonies on PYG medium, such as Figure 1 The colony diameter was 0.9-1.5 mm. The distinguishing physiological and biochemical characteristics of strain BI304 and its closely related strains are shown in Table 1.

[0062] Table 1. Distinctive physiological and biochemical characteristics of strain BI304 and its closely related strains

[0063]

[0064]

[0065] Note: + indicates positive, - indicates negative, and w indicates weakly positive.

[0066] 2. Detection of cytochemical composition of strain BI304

[0067] The cellular chemical components of strain BI304, such as fatty acids, quinone types, polar lipids, characteristic amino acids and sugar components, were detected by GC gas chromatography, HPLC liquid chromatography and TLC thin layer chromatography (Sasser M. Identification of bacteria by gas ghromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI inc; 1990. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233–241.). The fatty acid composition of strain BI3043 is shown in Table 2. The results show that the main fatty acid of strain BI304 is antesio-C 15:0 ,iso-C 16:0 and iso-C 15:0 ; In strain BI304, the cell wall contained LL-DAP, and the whole cell hydrolysate contained glucose and arabinose; the potential menaquinone was MK-9 (H4); diphosphatidylglycerol (DPG), polar lipid components included phosphatidylglycerol (PE), phosphatidylinositol (PC) and unknown glycophospholipids (GPL), such as Figure 2 shown.

[0068] Table 2. Fatty acid composition of strain BI304

[0069]

[0070]

[0071] In summary, the analysis results of the cell wall characteristic amino acids, sugar components, cell dominant fatty acid components, main respiratory quinones and polar lipid components of strain BI304 support the classification of strain BI304 into the genus Geoderma. At the same time, some physiological and biochemical characteristics (Table 1) and specific fatty acid composition (Table 2) of strain BI304 can be distinguished from other species of the genus Geoderma, supporting that strain BI304 represents a new species of the genus Geoderma.

[0072] 3. Determination of the phylogenetic status of strains

[0073] The genomic DNA of strain BI304 was extracted for sequencing, and the 16S rRNA gene sequence (SEQ ID No. 1) thereof was compared online in the international authoritative bacterial taxonomy analysis database (http: / / www.ezbiocloud.net / ) (Kim OS, Cho YJ, Lee K, et al. 2012, Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol, 62: 716-721.). The results showed that the strain BI304 of the present invention had the highest similarity with the species of the genus Geoderma. The highest similarity of the 16S rRNA gene sequence of the strain BI304 of the present invention with known species is: Geodermatophilus sabuli BMG 8133T (98.34%), Geodermatophilus ruber CPCC201356T (97.92%), Geodermatophilus obscurus DSM43160T (97.50%) and Geodermatophilus poikilotrophus DSM 44209T (97.50%). These similarity values ​​are all lower than the limit of 98.65% for distinguishing prokaryotic microbial species (Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequences similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64: 346–351.). The 16S rRNA gene sequences of all valid strains of the genus Geodermatophilus and some strains of the genera adjacent to the genus Geodermatophilus in the family Geodermataceae were selected to construct a phylogenetic tree ( Figure 3 The results showed that strain BI304 was a member of the genus Geodermatophilus and clustered with Geodermatophilus sabuli BMG 8133T and Geodermatophilus ruber CPCC201356T on a stable sub-evolutionary branch, and strain BI304 occupied the evolutionary position of a new species.

[0074] 4. Detection of alkaline phosphatase activity of strain BI304 and detection of stress resistance genes in its genome

[0075] The degradation activity of the strain BI304 of the present invention on organophosphorus compounds is determined by liquid screening method (Schrenkhammer P, Rosnizeck IC, Duerkop A, et al. Time-resolved fluorescence-based assay for the determination of alkaline phosphatase activity and application to the screening of its inhibitors [J]. Journal of Biomolecular Screening, 2007, 13 (1): 9-16.). An organophosphorus liquid culture medium is prepared, and the specific ingredients are as follows: lecithin 0.5 g·L -1 , glucose 10 g·L -1 , magnesium chloride hexahydrate 5g·L -1 , magnesium sulfate heptahydrate 0.25 g·L -1 , potassium chloride 0.2 g·L -1 , ammonium sulfate 0.1 g·L -1 , pH 7.2. The BI304 strain in the logarithmic growth phase was inoculated into 20 mL of liquid organic phosphorus culture medium, and a blank medium without bacteria was used as a control group. After culturing at 28°C for 10 days, the supernatant was centrifuged to determine the organic phosphorus concentration in the culture medium, and the blank group was used as a control to calculate the PO4 produced by the strain in degrading organic phosphorus. 3+ (mg / L) concentration change (using △PO4 3+ The standard curve was prepared according to the phosphate standard solution, and M0 was the PO4 detected in the blank control group. 3+ concentration, M1 is the PO4 detected in the experimental group 3+ concentration.

[0076] ΔPO4 3+ Concentration = M1-M0

[0077] The strain BI304 of the present invention has alkaline phosphatase activity and can degrade △PO4 produced by organic phosphorus. 3+ The concentration value was 12.11 mg / L (the average value obtained from three independent experiments).

[0078] At the genome level of strain BI304, it was detected that strain BI304 contained alkaline phosphatase A gene (pho A) and alkaline phosphatase D (pho D) gene. In summary, from the phenotypic and genotypic levels, it was proved that strain BI304 of the present invention had alkaline phosphatase activity.

[0079] Anti-UV genes (uvrA, uvrB, uvrC), DNA damage repair related genes (recO, recQ), carbon starvation tolerance related genes (carbon monoxide dehydrogenase, coxS2) related genes, etc. were detected from the genome of strain BI304 (Appendix 1). These genes lay the genetic material foundation for strain BI304 to perform pioneer biological functions in harsh habitats such as deserts. Combined with the phenotypic experiments and genotypic detection results of strain BI304 in terms of organic phosphorus degradation activity, that is, strain BI304 contains alkaline phosphatase A gene (pho A) and alkaline phosphatase D (pho D) gene. Therefore, strain BI304 of the present invention has broad application prospects in garbage disposal, soil remediation, desert control, soil quality improvement, etc.

[0080] In summary, the strain BI304 of the present invention represents a new species of Geodermatophilus, named Geodermatophilus resistens. At the same time, through the detection of phosphorus solubilization activity, it is also proved that the strain of the present invention has alkaline phosphatase activity, and is a functional new species of Geodermatophilus, which has broad application prospects in garbage disposal, soil remediation, desert control, soil quality improvement, etc.

[0081] Geodermatophilus resistens was deposited in the China General Microbiological Culture Collection Center on October 17, 2022, and its deposit number is CGMCC No.25291.

[0082] The present invention has been described in detail above. It will be apparent to those skilled in the art that the present invention may be implemented in a wide range under equivalent parameters, concentrations and conditions without departing from the spirit and scope of the present invention and without the need for unnecessary experimentation. Although the present invention provides specific embodiments, it should be understood that further improvements may be made to the present invention. In short, according to the principles of the present invention, this application intends to include any changes, uses or improvements to the present invention, including changes made by conventional techniques known in the art that depart from the scope disclosed in this application. Applications of some of the basic features may be made within the scope of the following appended claims.

Claims

1. Stress-resistant dermatophytes ( Geodermatophilus resistens ) BI304, whose registration number in the General Microbiological Center of China Culture Collection Administration is CGMCC No.25921.

2. The stress-resistant dermatophyte according to claim 1 ( Geodermatophilus resistens ) The culture of BI304 is the stress-resistant dermatophyte of claim 1 ( Geodermatophilus resistens ) Biologically pure culture of BI304.

3. A microbial agent, characterized in that: The bacterial agent contains the stress-resistant dermatophyte according to claim 1 ( Geodermatophilus resistens ) BI304 and / or the culture described in claim 2.

4. The bacterial agent according to claim 3, characterized in that: The bacterial agent is a bacterial agent that degrades organic phosphorus.

5. The stress-resistant geodermatophilic bacteria according to claim 1 ( Geodermatophilus resistens ) Use of BI304 or the culture of claim 2 or the bacterial agent of claim 3 or 4 in any of the following: (A1) Degradation of organic phosphorus; (A2) preparing products for the degradation of organic phosphorus; (A3) Preparing a product having alkaline phosphatase activity.

6. A product for degrading organic phosphorus, the active ingredient of which is the stress-resistant Geodermatophyte ( Geodermatophilus resistens ) BI304 or the culture described in claim 2 or the bacterial agent described in claim 3 or 4.

7. A product having alkaline phosphatase activity, wherein the active ingredient is the stress-resistant Geodermatophyte ( Geodermatophilus resistens ) BI304 or the culture described in claim 2 or the bacterial agent described in claim 3 or 4.

8. A method for degrading organic phosphorus, comprising the following steps: using the stress-resistant dermatophyte of claim 1 ( Geodermatophilus resistens ) The sample to be treated is treated with BI304 or the culture described in claim 2 or the bacterial agent described in claim 3 or 4.

9. The stress-resistant geodermatophyte according to claim 1 ( Geodermatophilus resistens ) Use of BI304 in the preparation of the culture described in claim 2 or the bacterial agent described in claim 3 or 4.

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