Quick scissors for cutting PVDF membrane before and after Western blotting

Abstract

The invention relates to the technical field of biological experimental equipment, and is a pair of quick scissors for cutting PVDF membranes before and after Western blotting mold transfer, comprising an upper handle and a lower handle arranged at intervals up and down, a base and an L-shaped shearing knife, and the lower right rear side of the upper handle and the upper right rear side of the lower handle are hinged together. The invention has a reasonable and compact structure and is easy to use. The opening angle of the L-shaped shearing knife can be quickly adjusted through a transverse support component and a longitudinal support component, and the blade positions in the transverse cutting component and the longitudinal cutting component can be quickly adjusted by correspondingly adjusting the extension length of the longitudinal adjustment rod and the transverse adjustment rod, so that the boundary of the target cutting area completely coincides with the boundary of the predetermined target area, and the predetermined target area is simply, quickly and accurately cut out on the PVDF membrane at one time; the opening angle of the L-shaped shearing knife is adjusted by the transverse support component and the longitudinal support component, which can effectively prevent the shearing range from being too large and wasting the PVDF membrane, and the shearing area selection is flexible.

Description

Technical Field

[0001] The invention relates to the technical field of biological experimental equipment, in particular to a pair of quick scissors used for cutting PVDF membranes before and after Western blotting mold transfer. Background Art

[0002] Immunoblotting, also known as protein blotting, is a method for detecting a protein in a complex sample based on the specific binding of antigens and antibodies. Western blotting is an experimental method often used in molecular biology, biochemistry and immunogenetics, and is a qualitative and semi-quantitative analysis method for proteins. It uses specific antibodies to stain cells or biological tissue samples treated with gel electrophoresis, and obtains information on the expression of specific proteins in the analyzed cells or tissues by analyzing the location and depth of staining. Combined with chemiluminescence detection, the expression differences of the same protein in multiple samples can be compared at the same time. The main steps of Western blotting include electrophoresis, transfer, blocking, hybridization and color development. After protein electrophoresis separation, the SDS-PAGE gel needs to be transferred to a membrane, that is, the target protein is transferred to a PVDF membrane for detection.

[0003] At present, the imported BlotCycler fully automatic protein hybridization instrument on the market can automatically perform the steps of blocking, hybridization, washing, etc. after membrane transfer, but compared with manual operation, it has many defects such as expensive instruments and huge amount of antibodies used, which cannot be applied to most laboratories. In manual operation, PVDF membrane is expensive. In order to save experimental costs, it is necessary to cut the PVDF membrane of matching size according to the size of SDS-PAGE gel before transfer; after transferring the protein from the gel to the PVDF membrane, in order to achieve the purpose of incubating multiple primary antibodies at the same time, the PVDF membrane needs to be cut according to the molecular weight of the target protein.

[0004] The Chinese patent document with the announcement number CN209440285U discloses a Western Blot membrane cutting device with a ruler, which includes a bottom plate and a knife holder. The bottom plate is provided with a ruler and an adjustable slot. The ruler is located on one side of the adjustable slot and forms a positioning frame with the adjustable slot for placing the SDS-PAGE gel to be cut. One side of the SDS-PAGE gel to be cut is aligned with the ruler and fixed by the adjustable slot. A Western Blot Marker ruler is designed on one side of the device to clarify the position of the target protein and prevent the target protein from being cut off; on the other hand, the knife is used to achieve fast and accurate membrane cutting.

[0005] A Chinese patent document with announcement number CN208663933U discloses a PVDF transfer film shearing device, which includes a main shearing plate with a rectangular structure, a first shearing component arranged on the side of the wide side line of the main shearing plate, and a second shearing component arranged on the side of the long side line of the main shearing plate, the bottom of the main shearing plate is provided with a plurality of bases distributed in a rectangular shape, the main shearing plate is provided with a plurality of criss-crossing scale grooves, a first accommodating plate for accommodating the first shearing component is provided on the side of the length line adjacent to the first shearing component of the main shearing plate, a second accommodating plate for accommodating the first shearing component is provided on the side of the width line adjacent to the second shearing component of the main shearing plate, a first accommodating groove is provided on the first accommodating plate, a second accommodating groove is provided on the second accommodating plate, the first shearing component can move along the width direction of the main shearing plate, and the second shearing component can move along the length direction of the main shearing plate; the plurality of scale grooves include a plurality of scale grooves along the width of the main shearing plate The invention relates to a plurality of widthwise scale grooves evenly spaced in the direction and a plurality of lengthwise scale grooves evenly spaced in the length direction of the main shear plate, each of the widthwise scale grooves and the lengthwise scale grooves penetrating the top of the main shear plate in the horizontal direction; an annular groove is provided on the side wall of the main shear plate, the contour line of the annular groove is the same as the contour line of the main shear plate, and the first shear component and the second shear component are both movably arranged in the annular groove; the first shear component comprises a first sliding portion, a first shear component arranged on the first sliding portion, and a first rotating shaft portion for connecting the first sliding portion and the first shear component, the first sliding portion comprises a first longitudinal sliding block, the first longitudinal sliding block is arranged in the annular groove and its end away from the inner side wall of the annular groove extends outwardly along the length direction of the main shear plate, the first rotating shaft portion comprises a first positioning rotating shaft and a first positioning pin arranged on the top of the first positioning rotating shaft, and the end of the first shear component close to the first longitudinal sliding block is rotatably arranged on the first positioning rotating shaft. It can slide according to the scale to facilitate cutting the corresponding PVDF membrane molecule marker and target protein. There are handles above the first and second cutters for easy pressing during cutting, so as to quickly, accurately and sharply cut the transfer membrane.

[0006] Therefore, the existing cutting devices used in PVDF membranes have the following shortcomings in actual use: the cutting process is cumbersome for PVDF membranes before and after transfer, and it is difficult to cut them all at once; the cutting range is too large, and it is difficult to cut only the predetermined target area on the PVDF membrane without wasting the PVDF membrane in one go; there is a lack of flexibility in randomly cutting the target protein after transfer, and the cutting process is too cumbersome, making it difficult to accurately cut according to the predetermined target area (i.e., the specifications and areas desired by the experimenter, which are usually multiple). Summary of the invention

[0007] The present invention provides a quick scissors for cutting PVDF membrane before and after Western blotting, which overcomes the shortcomings of the above-mentioned prior art and can effectively solve the problems of the existing PVDF membrane cutting device, such as the cumbersome process of cutting the PVDF membrane before and after mold transfer, the difficulty of cutting at one time, and the lack of flexibility in randomly cutting the target protein; it further solves the problem of wasting PVDF membrane due to too large a cutting range and the difficulty of accurately cutting according to the predetermined target area.

[0008] The technical scheme of the present invention is achieved through the following measures: a quick scissors for cutting PVDF membrane before and after Western blotting mold transfer, comprising an upper handle and a lower handle arranged at intervals above and below, a base and an L-shaped shearing knife, the lower side of the right rear portion of the upper handle and the upper side of the right rear portion of the lower handle are hinged together, the right rear end of the lower handle is fixedly installed with the outer side of the lower left corner of the base, a first shearing groove opening upward is provided on the upper side of the front end of the base, a second shearing groove opening upward and the front end connected to the left end of the first shearing groove is provided on the upper side of the left end of the base; the right rear end of the upper handle is fixedly installed with the outer side of the corner of the L-shaped shearing knife, the lower end of the front of the L-shaped shearing knife is located in the first shearing groove, and the lower end of the left portion of the L-shaped shearing knife is located in the second shearing groove; a transverse support component that can open the L-shaped shearing knife to a certain angle is slidably installed on the L-shaped shearing knife corresponding to the position above the second shearing groove, and a transverse sliding rod is detachably installed on the upper part of the transverse support component, corresponding to the L-shaped A guide slot which runs through the front and rear is provided on the transverse slide bar at the inner left side of the shearing knife; a longitudinal support assembly which enables the L-shaped shearing knife to open a certain angle is slidably installed on the L-shaped shearing knife at the position corresponding to the position above the first shearing slot; a longitudinal slide bar which is detachably installed on the upper part of the longitudinal support assembly and is located in the guide slot at the rear; a first longitudinal cutting assembly which can slide forward and backward along the longitudinal slide bar to cut the PVDF film is mounted on the outer side of the longitudinal slide bar which corresponds to the position between the transverse slide bar and the front part of the L-shaped shearing knife; a first transverse cutting assembly which can slide left and right along the transverse slide bar to cut the PVDF film is mounted on the outer side of the transverse slide bar which corresponds to the position between the longitudinal slide bar and the left part of the L-shaped shearing knife; the shearing path of the L-shaped shearing knife, the shearing path of the first longitudinal cutting assembly and the shearing path of the first transverse cutting assembly constitute a target cutting area which can cut a predetermined target area on the PVDF film at one time.

[0009] The following are further optimizations and / or improvements to the above technical solutions:

[0010] The above-mentioned longitudinal support assembly may include a longitudinal vertical shaft seat, a longitudinal fastening bolt, a longitudinal slider, and a longitudinal adjusting bolt. A longitudinal vertical shaft seat with a convex cross-section is provided on the outer side of the front end of the longitudinal slide bar. In the middle of the upper side of the longitudinal vertical shaft seat, there is a longitudinally penetrating fastening screw hole. Inside the longitudinally penetrating fastening screw hole, there is a longitudinal fastening bolt whose inner end abuts against the outer side of the longitudinal slide bar. Between the lower side of the longitudinal vertical shaft seat and the outer side of the upper part of the L-shaped shear blade, there is a U-shaped longitudinal slider with an opening downward. A longitudinal support platform is fixedly installed at the lower part of the front side of the longitudinal slider. There is a longitudinally penetrating adjusting screw hole on the longitudinal support platform. Inside the longitudinally penetrating adjusting screw hole, there is a longitudinal adjusting bolt whose lower end abuts against the upper side of the base. At the front side of the L-shaped shear blade corresponding to the lower position of the longitudinal slider, there is a first chute with an opening forward. At the rear side of the L-shaped shear blade corresponding to the position of the first chute, there is a second chute with an opening backward. Inside both the second chute and the first chute, there is a first guiding block whose outer end is fixedly installed at the corresponding position on the inner side of the longitudinal slider.

[0011] The above-mentioned transverse support assembly may include a transverse vertical shaft seat, a transverse fastening bolt, a transverse slider, and a transverse adjusting bolt. A transverse vertical shaft seat with a convex cross-section is provided on the outer side of the left end of the transverse slide bar. In the middle of the upper side of the transverse vertical shaft seat, there is a transversely penetrating fastening screw hole. Inside the transversely penetrating fastening screw hole, there is a transverse fastening bolt whose inner end abuts against the outer side of the transverse slide bar. Between the lower side of the transverse vertical shaft seat and the outer side of the upper part of the L-shaped shear blade, there is a U-shaped transverse slider with an opening downward. A transverse support platform is fixedly installed at the lower part of the left side of the transverse slider. There is a transversely penetrating adjusting screw hole on the transverse support platform. Inside the transversely penetrating adjusting screw hole, there is a transverse adjusting bolt whose lower end abuts against the upper side of the base. At the left side of the L-shaped shear blade corresponding to the lower position of the transverse slider, there is a third chute with an opening to the left. At the right side of the L-shaped shear blade corresponding to the position of the third chute, there is a fourth chute with an opening to the right. Inside both the fourth chute and the third chute, there is a second guiding block whose outer end is fixedly installed at the corresponding position on the inner side of the transverse slider.

[0012] The above-mentioned longitudinal slide bar may be a cylindrical slide bar. The longitudinal cutting assembly may include a longitudinal sliding sleeve, a longitudinal adjusting sleeve, a longitudinal adjusting rod, a longitudinal locking bolt, a longitudinal cutter bar sleeve, a longitudinal cutter bar, a longitudinal cutter seat, and a longitudinal cutter blade. A longitudinal sliding sleeve is sleeved on the outer side of the longitudinal slide bar. A longitudinal adjusting sleeve is fixedly installed on the left side of the longitudinal sliding sleeve. A longitudinal adjusting rod is sleeved inside the longitudinal adjusting sleeve. On the upper side of the left end of the longitudinal adjusting sleeve, there is a longitudinally penetrating locking screw hole. Inside the longitudinally penetrating locking screw hole, there is a longitudinal locking bolt whose inner end abuts against the upper side of the longitudinal adjusting rod. The left end of the longitudinal adjusting rod is fixedly installed with a longitudinal cutter bar sleeve whose left end is located on the right side of the L-shaped shear blade. There is a longitudinally penetrating guiding cutter bar hole in the middle of the longitudinal cutter bar sleeve. A longitudinal cutter bar is sleeved inside the longitudinally penetrating guiding cutter bar hole. The lower end of the longitudinal cutter bar is fixedly installed with a longitudinal cutter seat. There is a longitudinally and front-rear penetrating longitudinal cutter blade groove with an opening downward on the lower side of the longitudinal cutter seat. A longitudinal cutter blade whose lower end abuts against the upper side of the base is installed inside the longitudinal cutter blade groove.

[0013] The above-mentioned transverse sliding rod is a cylindrical sliding rod, and the transverse cutting assembly includes a transverse sliding sleeve, a transverse adjustment sleeve, a transverse adjustment rod, a transverse locking bolt, a transverse tool rod sleeve, a transverse tool rod, a transverse tool seat and a transverse blade. A transverse sliding sleeve is mounted on the outer side of the transverse sliding rod, and a transverse adjustment sleeve is fixedly installed on the front side of the transverse sliding sleeve, and a transverse adjustment rod is mounted inside the transverse adjustment sleeve. A transverse locking screw hole that passes through inside and outside is provided on the upper side of the transverse adjustment sleeve at the front end of the transverse adjustment sleeve, and a transverse locking bolt whose inner end abuts against the upper side of the transverse adjustment rod is provided in the transverse locking screw hole. A transverse tool rod sleeve whose front end is located behind the L-shaped shearing knife is fixedly installed at the front end of the transverse adjustment rod, a guide transverse tool rod hole that passes through up and down is provided in the middle part of the transverse tool rod sleeve, and a transverse tool rod is mounted in the guide transverse tool rod hole, and a transverse tool seat is fixedly installed on the lower end of the transverse tool rod, and a transverse blade groove that opens downward and passes through left and right is provided on the lower side of the transverse tool seat, and a transverse blade groove is installed in which the lower end of the transverse blade abuts against the upper side of the base.

[0014] The above-mentioned longitudinal cutting component may also include a first compression spring, a longitudinal anti-detachment block and a second compression spring. A first compression spring is provided on the outer side of the longitudinal knife rod corresponding to the position between the lower side of the longitudinal knife rod sleeve and the upper side of the longitudinal knife seat, and a longitudinal anti-detachment block is detachably installed on the upper end of the longitudinal knife rod; a first waist-shaped groove opening upward is provided on the longitudinal adjustment rod corresponding to the position of the longitudinal locking bolt, and the lower end of the longitudinal locking bolt is located in the first waist-shaped groove; a second compression spring is provided in the longitudinal adjustment sleeve corresponding to the right side position of the longitudinal adjustment rod, and a first copper sleeve or a first linear bearing is provided between the longitudinal sliding sleeve and the longitudinal sliding rod.

[0015] The above-mentioned transverse cutting component may also include a third compression spring, a transverse anti-slip block and a fourth compression spring. A third compression spring is provided on the outer side of the transverse knife rod corresponding to the position between the lower side of the transverse knife rod sleeve and the upper side of the transverse knife seat, and a transverse anti-slip block is detachably installed on the upper end of the transverse knife rod; a second waist-shaped groove opening upward is provided on the transverse adjustment rod corresponding to the position of the transverse locking bolt, and the lower end of the transverse locking bolt is located in the second waist-shaped groove; a fourth compression spring is provided in the transverse adjustment sleeve corresponding to the rear side of the transverse adjustment rod, and a second copper sleeve or a second linear bearing is provided between the inner side of the transverse sliding sleeve and the outer side of the transverse sliding rod.

[0016] A support assembly can be slidably installed on the outer side of the right part of the above-mentioned transverse sliding rod, and the support assembly may include a support sleeve, a tightening bolt, an inner sleeve rod, a fifth compression spring, an outer sleeve, a flexible foot and a locking bolt. A support sleeve is mounted on the outer side of the right part of the transverse sliding rod, and a tightening screw hole that passes through inside and outside is provided on the upper side of the support sleeve, and a tightening bolt whose inner end abuts against the outer side of the transverse sliding rod is provided in the tightening screw hole; an inner sleeve rod is fixedly installed on the lower side of the support sleeve, and an outer sleeve is mounted on the outer side of the inner sleeve rod, and a flexible foot seat whose lower end abuts against the upper side of the base is fixedly installed on the lower side of the outer sleeve, and a fifth compression spring is provided in the outer sleeve corresponding to the position between the flexible foot seat and the inner sleeve rod; a locking screw hole that passes through inside and outside is provided on the right side of the upper part of the outer sleeve, and an anti-slip groove that opens outward and is waist-shaped is provided on the inner sleeve rod corresponding to the position of the locking screw hole, and a locking bolt with the inner end and the anti-slip groove is provided in the locking screw hole.

[0017] The lower right rear side of the upper handle and the upper right rear side of the lower handle can be hinged together through a hinge shaft, and a torsion spring can be provided on the outer side of the hinge shaft corresponding to the position between the upper handle and the lower handle. The rear end and right end of the L-shaped shear knife can be removably installed with a first baffle, and the right end of the transverse sliding rod can be removably installed with a second baffle.

[0018] A coordinate grid may be provided on the upper side of the base at the position between the first shearing groove and the second shearing groove, a longitudinal scale may be provided on the upper side of the longitudinal slide bar at the position of the coordinate grid, a transverse scale may be provided on the upper side of the transverse slide bar at the position of the coordinate grid, and two mutually perpendicular shearing scales may be provided on the upper side of the L-shaped shearing knife, and the zero points of the two shearing scales are located at the corner position of the L-shaped shearing knife.

[0019] The present invention has a reasonable and compact structure and is easy to use. The opening angle of the L-shaped shearing knife can be quickly adjusted through the transverse support assembly and the longitudinal support assembly. The blade positions in the transverse cutting assembly and the longitudinal cutting assembly can be quickly adjusted by correspondingly adjusting the extended lengths of the longitudinal adjustment rod and the transverse adjustment rod, so that the boundary of the target cutting area is completely coincident with the boundary of the predetermined target area, and the predetermined target area can be simply, quickly and accurately cut out on the PVDF membrane at one time; the opening angle of the L-shaped shearing knife can be adjusted by the transverse support assembly and the longitudinal support assembly, so that the shearing range is too large and waste of PVDF membrane can be effectively prevented, and the cutting process is flexible, simple and easy to implement with high cutting accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Attached Figure 1 It is a schematic diagram of the top structure of embodiment 1 of the present invention.

[0021] Attached Figure 2 For attachment Figure 1 Schematic diagram of the three-dimensional structure.

[0022] Attached Figure 3 For attachment Figure 1 Schematic diagram of the cross-sectional structure of the middle support assembly enlarged at AA.

[0023] Attached Figure 4 For attachment Figure 1 Schematic diagram of the three-dimensional structure of the longitudinal support assembly.

[0024] Attached Figure 5 For attachment Figure 1 Schematic diagram of the three-dimensional structure of the middle transverse support assembly.

[0025] Attached Figure 6 For attachment Figure 1 Schematic diagram of the three-dimensional partial cross-sectional structure of the longitudinal cutting component.

[0026] Attached Figure 7 For attachment Figure 1 Schematic diagram of the three-dimensional partial cross-sectional structure of the middle transverse cutting component.

[0027] The codes in the accompanying drawings are: 1 is an upper handle, 2 is a lower handle, 3 is a base, 4 is an L-shaped shearing knife, 5 is a hinge shaft, 6 is a first shearing groove, 7 is a second shearing groove, 8 is a horizontal vertical shaft seat, 9 is a horizontal slide bar, 10 is a guide slide groove, 11 is a longitudinal slide bar, 12 is a horizontal sliding sleeve, 13 is a longitudinal vertical shaft seat, 14 is a longitudinal fastening bolt, 15 is a longitudinal slider, 16 is a longitudinal adjustment bolt, 17 is a longitudinal support platform, 18 is a first slide groove, 19 is a fourth slide groove, 20 is a first guide block, 21 is a longitudinal sliding sleeve, 22 is a longitudinal adjustment sleeve, 23 is a longitudinal adjustment rod, 24 is a longitudinal locking bolt, 25 is a longitudinal knife rod sleeve, 26 is a longitudinal knife rod, 27 is a longitudinal knife seat, 28 is a longitudinal blade, 29 is a first compression spring, 30 is a first waist groove, 31 is a second compression spring, 32 is a first linear bearing, 33 is a supporting sleeve, 34 is a set bolt, 35 is an inner sleeve rod, 36 is a fifth compression spring, 37 is an outer sleeve, 38 is a flexible foot seat, 39 is a locking bolt, 40 is a torsion spring, 41 is a first baffle, 42 is a second baffle, 43 is a coordinate grid, 44 is a longitudinal scale, 45 is a transverse scale, 46 is a shear scale, 47 is a longitudinal anti-slip block, 48 is an anti-slip groove, 49 is a predetermined target area, 50 is a transverse fastening bolt, 51 is a transverse slider, 52 is a transverse adjustment bolt, 53 is a transverse support platform, 54 is a second guide block, 55 is a transverse adjustment sleeve, 56 is a transverse adjustment rod, 57 is a transverse locking bolt, 58 is a transverse tool rod sleeve, 59 is a transverse tool rod, 60 is a transverse tool seat, 61 is a transverse blade, 62 is a third compression spring, 63 is a transverse anti-slip block, 64 is a fourth compression spring, 65 is a second waist groove, and 66 is a second linear bearing. DETAILED DESCRIPTION

[0028] The present invention is not limited by the following embodiments, and specific implementation methods can be determined based on the technical solution of the present invention and actual conditions.

[0029] In the present invention, for the convenience of description, the relative position relationship of each component is described according to the attached Figure 1 The positional relationships such as front, back, top, bottom, left, and right are described according to the layout directions of the drawings in the specification.

[0030] The present invention will be further described below in conjunction with embodiments and drawings:

[0031] Embodiment 1: As attached Figure 1 , 2 As shown in Figures 3, 4, 5, 6 and 7, the quick scissors for cutting PVDF membrane before and after Western blotting die transfer comprise an upper handle 1 and a lower handle 2, a base 3 and an L-shaped shearing knife 4 which are spaced apart from each other. The lower right rear side of the upper handle 1 and the upper right rear side of the lower handle 2 are hinged together. The right rear end of the lower handle 2 is fixedly mounted to the outer side of the lower left corner of the base 3. The upper front end of the base 3 is provided with a first shearing groove 6 which opens upward. The upper left end of the base 3 is provided with a second shearing groove 7 which opens upward and the front end is connected to the left end of the first shearing groove 6. The right rear end of the upper handle 1 is fixedly mounted to the outer side of the corner of the L-shaped shearing knife 4. The lower front end of the L-shaped shearing knife 4 is located in the first shearing groove 6, and the lower left end of the L-shaped shearing knife 4 is located in the second shearing groove 7. A transverse supporting assembly which enables the L-shaped shearing knife 4 to open a certain angle is slidably mounted on the L-shaped shearing knife 4 at a position above the second shearing groove 7. A transverse sliding rod 9 is detachably mounted on the upper part of the transverse supporting assembly. A guide slot 10 which runs through the front and rear is provided on the transverse slide bar 9 at the inner left side of the shearing knife 4; a longitudinal support assembly which enables the L-shaped shearing knife 4 to open a certain angle is slidably installed on the L-shaped shearing knife 4 at the position above the corresponding first shearing slot 6; a longitudinal slide bar 11 which is detachably installed on the upper part of the longitudinal support assembly and is located in the guide slot 10 at the rear; a first longitudinal cutting assembly which can slide forward and backward along the longitudinal slide bar 11 to cut the PVDF film is mounted on the outer side of the longitudinal slide bar 11 which is located between the corresponding transverse slide bar 9 and the front part of the L-shaped shearing knife 4; a first transverse cutting assembly which can slide left and right along the transverse slide bar 9 to cut the PVDF film is mounted on the outer side of the transverse slide bar 9 which is located between the corresponding longitudinal slide bar 11 and the left part of the L-shaped shearing knife 4; the shearing path of the L-shaped shearing knife 4, the shearing path of the longitudinal cutting assembly and the shearing path of the transverse cutting assembly constitute a target cutting area which can cut a predetermined target area 49 on the PVDF film at one time.

[0032] According to the needs, the first shearing groove 6 and the second shearing groove 7 can form an L-shaped shearing groove that matches the shape of the L-shaped shearing knife 4. In order to facilitate the lower part of the L-shaped shearing knife 4 to quickly enter the L-shaped shearing groove, the inner side and the outer side of the lower end of the L-shaped shearing knife 4 are provided with guide fillets or guide chamfers. The L-shaped shearing knife 4 can be opened upward by pinching the upper handle 1 and the left front part of the lower handle 2. After releasing, the L-shaped shearing knife 4 can be closed downward and gradually enter the L-shaped shearing groove. The opening and closing operation of the L-shaped shearing knife 4 is easy, simple and fast. The first transverse cutting component can be installed by an outer sliding sleeve on the outer side of the transverse sliding rod 9 and installed on the outer sliding sleeve. The movable longitudinal knife bar and the cutting knife installed at the lower end of the longitudinal knife bar can be realized, or can be realized by an outer sliding sleeve, a longitudinal knife bar that can be telescopically adjusted in length (a longitudinal knife bar with multiple sections fitted together) and a cutting knife, or can be directly realized based on a linear guide rail in the prior art, that is, the transverse slide bar 9 is realized by a guide rail, and the slider on the guide rail cooperates with the longitudinal knife bar and the cutting knife to realize the sliding cutting function. The structure of the first longitudinal cutting component can be the same as that of the first transverse cutting component, or a different structure can be selected to ensure that the blade of the cutting knife can reliably resist the boundary of the target cutting area on the PVDF membrane for sliding cutting; when After the longitudinal slide bar 11 and the transverse slide bar 9 slide to the predetermined position, the lower ends of the transverse support assembly and the longitudinal support assembly can form a support with the upper side of the base 3, so that the L-shaped shearing knife 4 is opened at a certain angle, avoiding the right front and left rear of the L-shaped shearing knife 4 from contacting the upper end of the inner wall of the L-shaped shearing groove, and preventing the L-shaped shearing knife 4 from having an excessively large shearing range, and cutting the remaining PVDF film in one go, causing waste (in order to completely cut the front boundary and the left boundary of the predetermined target area 49, the size of the target cutting area is slightly larger than the size of the predetermined target area 49 at this time). In addition, in order to quickly Fixed at a predetermined position on the L-shaped shearing knife 4, both the transverse support assembly and the longitudinal support assembly can be screwed with a fastening top screw; the sliding connection between the transverse support assembly, the longitudinal support assembly and the L-shaped shearing knife 4 can be achieved by the slider and guide rail structure in the linear guide rail in the existing known technology, or directly through the linear guide rail, or dovetail groove and dovetail slider, or T-slot and T-slide, etc. In addition, in order to make the L-shaped shearing knife 4 open to a certain angle, the transverse support assembly and the longitudinal support assembly can be achieved by setting a support column or a support wedge or a plunger or a card table, and the two can use the same structure or different structures.

[0033] During use, first adjust the positions of the horizontal support assembly and the vertical support assembly according to the size and shape of the predetermined target area 49, so that the L-shaped shear blade 4 opens at a certain angle, and then fix the relative positions between the two and the L-shaped shear blade 4; then lift the L-shaped shear blade 4, and then place the predetermined target area 49 to be cut on the PVDF film at the lower left corner on the upper side of the base 3, and align the lower left corner of the predetermined target area 49 with the corner position of the L-shaped cutting groove, and then loosen the upper handle 1 and the lower handle 2 (at this time, the left boundary and the front boundary of the target cutting area coincide with the left boundary and the front boundary of the predetermined target area 49, the right boundary of the target cutting area is to the right of the predetermined target area 49, and the rear boundary of the target cutting area is behind the predetermined target area 49, that is, the target cutting area is slightly larger than the predetermined target area 49). Press down the L-shaped shear blade 4, and the left boundary and the front boundary of the predetermined target area 49 can be quickly cut through the friction between the inner side of the L-shaped shear blade 4 and the inner side of the L-shaped shear groove. Then slide the first horizontal cutting assembly left and right and the first vertical cutting assembly up and down respectively, and the remaining two sides of the predetermined target area 49 can be quickly cut out. The structure of the present invention is reasonable and compact, and it is convenient to use. It can quickly adjust the opening angle of the L-shaped shear blade 4 through the horizontal support assembly and the vertical support assembly, so that the target cutting area is slightly larger than the predetermined target area 49, and the predetermined target area 49 can be simply, quickly and accurately cut out at one time on the PVDF film.

[0034] According to actual needs, the above-mentioned quick scissors for cutting PVDF film before and after Western blotting transfer can be further optimized and / or improved:

[0035] Example 2: As shown in Figure 1 , 2 , Figure 4, the vertical support assembly includes a vertical vertical shaft seat 13, a vertical fastening bolt 14, a vertical slider 15 and a vertical adjusting bolt 16. A vertical vertical shaft seat 13 with a convex cross-section is provided on the outer side of the front end of the vertical slide rod 11. A vertically penetrating fastening screw hole is provided in the middle of the upper side of the vertical vertical shaft seat 13. A vertical fastening bolt 14 with an inner end abutting against the outer side of the vertical slide rod 11 is provided in the vertically penetrating fastening screw hole. A U-shaped vertical slider 15 with a downward opening is provided between the lower side of the vertical vertical shaft seat 13 and the outer side of the upper part of the L-shaped shear blade 4. A vertical support table 17 is fixedly installed at the lower front part of the vertical slider 15. A vertically penetrating vertical adjusting screw hole is provided on the vertical support table 17. A vertical adjusting bolt 16 with a lower end abutting against the upper side of the base 3 is provided in the vertically penetrating vertical adjusting screw hole. A first chute 18 with a forward opening is provided on the front side of the L-shaped shear blade 4 corresponding to the lower part of the vertical slider 15. A second chute with a backward opening is provided on the rear side of the L-shaped shear blade 4 corresponding to the first chute 18. First guide blocks 20 with outer ends fixedly installed at corresponding positions on the inner side of the vertical slider 15 are provided in both the second chute and the first chute 18.

[0036] During use, the longitudinal slide bar 11 is first slid to the vicinity of the predetermined position according to the position and size of the predetermined target area 49, and then the longitudinal adjustment bolt 16 is screwed to adjust the opening angle between the base 3 and the L-shaped shearing knife 4 by adjusting the length thereof extending out from the lower side of the longitudinal support platform 17, so that the right front part of the L-shaped shearing knife 4 is separated from the notch of the first shearing groove 6, so that the shearing range of the L-shaped shearing knife 4 is just slightly larger than the predetermined target area 49, and the predetermined target area 49 can be completely cut without cutting too much of the PVDF membrane outside the predetermined target area 49, so as to ensure the integrity of the remaining PVDF membrane as much as possible, avoid the subsequent use affected by excessive fragmentation of the remaining PVDF membrane, and save experimental costs. The longitudinal adjustment bolt 16 can also limit the relative position between the longitudinal slider 15 and the L-shaped shearing knife 4, so as to prevent the longitudinal slider 15 from sliding toward the corner position of the L-shaped shearing knife 4 due to its own weight when the L-shaped shearing knife 4 is opened to a predetermined angle, thereby affecting the relative position of the longitudinal slide bar 11 and preventing the longitudinal cutting assembly from deviating from the predetermined cutting position; the longitudinal slider 15 and the L-shaped shearing knife 4 can form a linear guide rail in the prior art, thereby realizing the sliding installation between the longitudinal slide bar 11 and the L-shaped shearing knife 4; when the L-shaped shearing knife 4 is opened, the first slide groove 18 and the second slide groove cooperate with the first guide block 20 installed on the inner side of the longitudinal slider 15 to effectively prevent the longitudinal slider 15 from detaching from the L-shaped shearing knife 4. According to the needs, in order to facilitate the experimenter to quickly turn the longitudinal adjustment bolt 16 by hand, the longitudinal adjustment bolt 16 can be realized by a hand-tightening bolt or a butterfly bolt in the prior art; the structure of the longitudinal vertical shaft seat 13 is the prior art, which is mainly used to install and fix the shaft end.

[0037] Embodiment 3: As attached Figure 1 , 2 As shown in Figure 5, the transverse support assembly may include a transverse vertical shaft seat 8, a transverse fastening bolt 50, a transverse slider 51 and a transverse adjustment bolt 52. A transverse vertical shaft seat 8 with a convex cross-section is provided on the outer side of the left end of the transverse slide bar 9. A transverse fastening screw hole that passes through the inside and outside is provided in the middle of the upper side of the transverse vertical shaft seat 8. A transverse fastening bolt 50 whose inner end abuts against the outer side of the transverse slide bar 9 is provided in the transverse fastening screw hole. A transverse slider 51 with a convex cross-section and opening downward is provided between the lower side of the transverse vertical shaft seat 8 and the outer side of the upper part of the L-shaped shearing knife 4. The left side of the transverse slider 51 A transverse support platform 53 is fixedly installed on the lower side, and a transverse adjustment screw hole is provided on the transverse support platform 53 which passes through from top to bottom. A transverse adjustment bolt 52 is provided in the transverse adjustment screw hole, and the lower end of the transverse adjustment bolt 52 abuts against the upper side of the base 3. A third slide groove opening to the left is provided on the left side of the L-shaped shearing knife 4 corresponding to the lower position of the transverse slider 51, and a fourth slide groove 19 opening to the right is provided on the right side of the L-shaped shearing knife 4 corresponding to the position of the third slide groove. A second guide block 54 is fixedly installed at the outer end at the corresponding position of the inner side of the transverse slider 51 in the fourth slide groove 19 and the third slide groove.

[0038] During use, the horizontal slide bar 9 is first slid to the vicinity of the predetermined position according to the position and size of the predetermined target area 49, and then the horizontal adjustment bolt 52 is screwed to adjust the opening angle between the base 3 and the L-shaped shearing knife 4 by adjusting the length thereof extending out from the lower side of the horizontal support platform 53, so that the upper left portion of the L-shaped shearing knife 4 is separated from the notch of the second shearing groove 7, so that the shearing range of the L-shaped shearing knife 4 is just slightly larger than the predetermined target area 49, and the predetermined target area 49 can be completely cut without cutting too much of the PVDF membrane outside the predetermined target area 49, so as to ensure the integrity of the remaining PVDF membrane as much as possible, avoid the subsequent use affected by excessive fragmentation of the remaining PVDF membrane, and save experimental time. Cost; the transverse adjusting bolt 52 can also limit the relative position between the transverse slider 51 and the L-shaped shearing knife 4, to prevent the transverse slider 51 from sliding toward the corner position of the L-shaped shearing knife 4 due to its own weight when the L-shaped shearing knife 4 is opened to a predetermined angle, thereby affecting the relative position of the transverse slide bar 9 and preventing the transverse cutting assembly from deviating from the predetermined cutting position; the transverse slider 51 and the L-shaped shearing knife 4 can form a linear guide rail in the prior art, thereby realizing the sliding installation between the transverse slide bar 9 and the L-shaped shearing knife 4; when the L-shaped shearing knife 4 is opened, the third slide groove and the fourth slide groove 19 cooperate with the second guide block 54 installed on the inner side of the transverse slider 51 to effectively prevent the transverse slider 51 from detaching from the L-shaped shearing knife 4. According to the needs, in order to facilitate the experimenters to quickly turn the transverse adjustment bolt 52 by hand, the transverse adjustment bolt 52 can be realized by a hand-tightened bolt or a butterfly bolt in the existing known technology; since the outer diameter of the transverse slide bar 9 is larger than the outer diameter of the longitudinal slide bar 11, the size of the transverse vertical shaft seat 8 in the transverse support assembly is larger than the size of the transverse vertical shaft seat 8 in the transverse support assembly. The structure of the transverse vertical shaft seat 8 is an existing known technology, which is mainly used for installing and fixing the shaft end.

[0039] Embodiment 4: As attached Figure 1 , 26, the longitudinal slide bar 11 can be a cylindrical slide bar, and the longitudinal cutting assembly includes a longitudinal sliding sleeve 21, a longitudinal adjustment sleeve 22, a longitudinal adjustment rod 23, a longitudinal locking bolt 24, a longitudinal knife bar sleeve 25, a longitudinal knife bar 26, a longitudinal knife seat 27 and a longitudinal blade 28. The longitudinal sliding sleeve 21 is sleeved on the outer side of the longitudinal slide bar 11, and the longitudinal adjustment sleeve 22 is fixedly installed on the left side of the longitudinal sliding sleeve 21. The longitudinal adjustment rod 23 is sleeved inside the longitudinal adjustment sleeve 22. The upper side of the left end of the longitudinal adjustment sleeve 22 is provided with a longitudinal locking screw hole that passes through inside and outside. A longitudinal locking bolt 24 is provided in the locking screw hole, and the inner end of the longitudinal adjusting rod 23 is abutted against the upper side of the longitudinal adjusting rod 23. A longitudinal knife rod sleeve 25 is fixedly installed on the left end of the longitudinal adjusting rod 23, and the left end of the longitudinal knife rod sleeve 25 is located on the right side of the L-shaped shearing knife 4. A longitudinal guide knife rod hole is provided in the middle of the longitudinal knife rod sleeve 25, which passes through from top to bottom. A longitudinal knife rod 26 is sleeved in the longitudinal guide knife rod hole. A longitudinal knife seat 27 is fixedly installed on the lower end of the longitudinal knife rod 26. A longitudinal blade groove with an opening downward and passing through front and back is provided on the lower side of the longitudinal knife seat 27. A longitudinal blade 28 with a lower end abutting against the upper side of the base 3 is installed in the longitudinal blade groove.

[0040] By such arrangement, the longitudinal sliding sleeve 21 can slide and rotate along the outer side of the cylindrical longitudinal sliding rod 11, and the longitudinal sliding sleeve 21 can be rotated to make the longitudinal knife bar 26 perpendicular to the upper side of the base 3, and the relative position of the longitudinal knife bar 26 can be adjusted left and right by pushing and pulling the longitudinal adjustment rod 23, so that the blade of the longitudinal blade 28 is perpendicular to and coincides with the right boundary of the predetermined target area 49, which is convenient for accurately cutting the right boundary of the predetermined target area 49, and the longitudinal locking bolt 24 can quickly fix the relative position between the longitudinal adjustment rod 23 and the longitudinal adjustment sleeve 22, so as to prevent the longitudinal adjustment rod 23 from moving and affecting the cutting accuracy during the cutting of the PVDF membrane; the longitudinal knife bar sleeve 25 is used to support the longitudinal knife bar 26, and the longitudinal guide knife bar hole has a guiding function, which can effectively prevent the longitudinal blade 28 from deviating from the right boundary of the predetermined target area 49 due to the rotation of the longitudinal knife bar 26 during the cutting of the PVDF membrane, thereby affecting the cutting accuracy. According to the requirements, its cross-section can be drum-shaped, square, pentagonal or hexagonal, and the shape of the longitudinal knife bar 26 matches the longitudinal guide knife bar hole. In this embodiment, a drum-shaped longitudinal knife bar is used.

[0041] During use, the longitudinal knife rod 26 is adjusted according to the right boundary position of the predetermined target area 49, so that the blade of the longitudinal blade 28 is perpendicular to the PVDF membrane and completely overlaps with the right boundary of the predetermined target area 49 (that is, the target cutting area completely overlaps with the predetermined target area 49). The longitudinal knife rod 26 is pushed forward and backward to quickly and accurately cut the right boundary of the predetermined target area 49 according to the predetermined size requirements; to ensure the sharpness of the longitudinal blade 28, the longitudinal blade 28 can use a replaceable disposable longitudinal blade, so that the fixing method between the longitudinal blade 28 and the longitudinal adjustment sleeve 22 can be achieved by a threaded fastener, so as to quickly disassemble and replace the longitudinal blade 28; affected by the length of the longitudinal sliding sleeve 21, to ensure that the longitudinal blade 28 can completely cut the predetermined target area 49, the length of the longitudinal blade 28 usually needs to be greater than the length of the longitudinal sliding sleeve 21.

[0042] Embodiment 5: As attached Figure 1 , 2 As shown in Figures 7 and 8, the transverse slide bar 9 is a cylindrical slide bar. The transverse cutting assembly includes a transverse sliding sleeve 12, a transverse adjustment sleeve 55, a transverse adjustment rod 56, a transverse locking bolt 57, a transverse knife bar sleeve 58, a transverse knife bar 59, a transverse knife seat 60 and a transverse blade 61. The transverse sliding sleeve 12 is sleeved on the outer side of the transverse slide bar 9, and the transverse adjustment sleeve 55 is fixedly installed on the front side of the transverse sliding sleeve 12. The transverse adjustment rod 56 is sleeved inside the transverse adjustment sleeve 55. The upper side of the front end of the transverse adjustment sleeve 55 is provided with a transverse locking screw hole that passes through inside and outside. The transverse locking screw A transverse locking bolt 57 is provided in the hole, and the inner end of the transverse locking bolt 57 abuts against the upper side of the transverse adjustment rod 56. A transverse knife rod sleeve 58 is fixedly installed at the front end of the transverse adjustment rod 56, and the front end of the transverse knife rod sleeve 58 is located behind the L-shaped shearing knife 4. A guide transverse knife rod 59 hole that passes through the upper and lower parts is provided in the middle part of the transverse knife rod sleeve 58. The transverse knife rod 59 is sleeved in the guide transverse knife rod 59 hole. A transverse knife seat 60 is fixedly installed at the lower end of the transverse knife rod 59. A transverse blade groove that opens downward and passes through the left and right parts is provided on the lower side of the transverse knife seat 60. A transverse blade 61 whose lower end abuts against the upper side of the base is installed in the transverse blade groove.

[0043] By such arrangement, the transverse sliding sleeve 12 can slide and rotate along the outer side of the cylindrical transverse sliding rod 9. The transverse knife bar 59 can be made perpendicular to the upper side of the base 3 by rotating the transverse sliding sleeve 12. The relative position of the transverse knife bar 59 can be adjusted forward and backward by pushing and pulling the transverse adjustment rod 56, so that the blade of the transverse blade 61 is perpendicular to and coincides with the rear boundary of the predetermined target area 49, which is convenient for accurately cutting the rear boundary of the predetermined target area 49. The transverse locking bolt 57 can quickly fix the relative position between the transverse adjustment rod 56 and the transverse adjustment sleeve 55, so as to prevent the transverse adjustment rod 56 from moving and affecting the cutting accuracy during the cutting of the PVDF membrane. The transverse knife bar sleeve 58 is used to support the transverse knife bar 59. The transverse guide knife bar hole has a guiding function, which can effectively prevent the transverse blade 61 from deviating from the right boundary of the predetermined target area 49 due to the rotation of the transverse knife bar 59 during the cutting of the PVDF membrane, thereby affecting the cutting accuracy. According to the requirements, its cross-section can be drum-shaped, square, pentagonal or hexagonal. The shape of the transverse knife bar 59 matches the transverse guide knife bar hole. In this embodiment, a drum-shaped transverse knife bar 59 is used.

[0044] During use, the position of the transverse knife rod 59 of the transverse cutting assembly is adjusted according to the rear boundary position of the predetermined target area 49, so that the blade of the transverse blade 61 is both perpendicular to the PVDF film and completely overlaps with the rear boundary of the predetermined target area 49 (so that the target cutting area completely overlaps with the predetermined target area 49), and the rear boundary of the predetermined target area 49 can be quickly and accurately cut according to the predetermined size requirements by pushing the transverse knife rod 59 left and right; to ensure the sharpness of the transverse blade 61, the transverse blade 61 can use a replaceable disposable transverse blade 61, so the fixing method between the transverse blade 61 and the transverse adjustment sleeve 55 can be achieved by a threaded fastener, so as to quickly disassemble and replace the transverse blade 61; affected by the length of the transverse sliding sleeve 12, in order to ensure that the transverse blade 61 can completely cut the predetermined target area 49, the length of the transverse blade 61 usually needs to be greater than the length of the transverse sliding sleeve 12; because the outer diameter of the transverse sliding rod 9 is larger than the outer diameter of the transverse sliding rod 9, the inner and outer diameter dimensions of the transverse sliding sleeve 12 are larger than the inner and outer diameter dimensions of the longitudinal sliding sleeve 21.

[0045] Embodiment 6: As attached Figure 1 , 2As shown in , 6, the longitudinal cutting assembly also includes a first compression spring 29, a longitudinal anti-detachment block 47 and a second compression spring 31. The first compression spring 29 is provided on the outer side of the longitudinal knife rod 26 at a position between the lower side of the longitudinal knife rod sleeve 25 and the upper side of the longitudinal knife seat 27, and the longitudinal anti-detachment block 47 is detachably installed on the upper end of the longitudinal knife rod 26; a first waist-shaped groove 30 opening upward is provided on the longitudinal adjustment rod 23 corresponding to the position of the longitudinal locking bolt 24, and the lower end of the longitudinal locking bolt 24 is located in the first waist-shaped groove 30; a second compression spring 31 is provided in the longitudinal adjustment sleeve 22 corresponding to the right side position of the longitudinal adjustment rod 23; a first copper sleeve or a first linear bearing 32 is provided between the longitudinal sliding sleeve 21 and the longitudinal sliding rod 11.

[0046] During use, the first compression spring 29 can make the longitudinal blade 28 always abut against the upper side of the base 3 (or PVDF membrane), which is more conducive to smooth cutting; when the longitudinal locking bolt 24 is loosened, the second compression spring 31 can make the longitudinal adjustment rod 23 automatically pop out to the left, so that the experimenter can quickly make the blade of the longitudinal blade 28 coincide with the right boundary of the predetermined target area 49 when pushing the longitudinal adjustment rod 23 to the right, thereby improving the adjustment efficiency. At this time, the lower end of the longitudinal locking bolt 24 abuts against the bottom of the first waist-shaped groove 30 to prevent the longitudinal adjustment rod 23 from popping out to the left under the elastic force of the second compression spring 31, and the first The waist-shaped groove 30 can prevent the longitudinal adjustment sleeve 22 and the longitudinal adjustment rod 23 from separating and disengaging under the spring restoring force of the second compression spring 31; the first copper sleeve or the first linear bearing 32 can make the longitudinal sliding sleeve 21 slide along the outer side of the longitudinal sliding rod 11 smoother, more stable and more wear-resistant. In order to improve the lubrication effect, the first copper sleeve can use a graphite self-lubricating copper sleeve; the longitudinal anti-detachment block 47 can prevent the longitudinal tool rod 26 from separating from the longitudinal tool rod sleeve 25 under the spring restoring force of the first compression spring 29. The longitudinal anti-detachment block 47 can be installed on the longitudinal tool rod 26 through threads or threaded fasteners in the existing known technology.

[0047] Embodiment 7: As attached Figure 1 , 2 As shown in , 7, the transverse cutting assembly may also include a third compression spring 62, a transverse anti-detachment block 63 and a fourth compression spring 64. A third compression spring 62 is provided on the outer side of the transverse knife rod 59 corresponding to the position between the lower side of the transverse knife rod sleeve 58 and the upper side of the transverse knife seat 60, and a transverse anti-detachment block 63 is detachably installed on the upper end of the transverse knife rod 59; a second waist-shaped groove 65 opening upward is provided on the transverse adjustment rod 56 corresponding to the position of the transverse locking bolt 57, and the lower end of the transverse locking bolt 57 is located in the second waist-shaped groove 65, and a fourth compression spring 64 is provided in the transverse adjustment sleeve 55 corresponding to the rear side position of the transverse adjustment rod 56, and a second copper sleeve or a second linear bearing 66 is provided between the inner side of the transverse sliding sleeve 12 and the outer side of the transverse sliding rod 9.

[0048] During use, the third compression spring 62 can make the transverse blade 61 always abut against the upper side of the base 3 (or PVDF membrane), which is more conducive to smooth cutting; when the transverse locking bolt 57 is loosened, the fourth compression spring 64 can make the transverse adjustment rod 56 automatically pop out forward, so that the experimenter can quickly make the blade of the transverse blade 61 coincide with the rear boundary of the predetermined target area when pushing the transverse adjustment rod 56 backward, thereby improving the adjustment efficiency. At this time, the lower end of the transverse locking bolt 57 abuts against the bottom of the second waist-shaped groove 65 to prevent the transverse adjustment rod 56 from popping out to the left under the elastic force of the fourth compression spring 64, and the second waist-shaped groove 65 can be prevented from being ejected. The groove 65 can prevent the lateral adjustment sleeve 55 and the lateral adjustment rod 56 from separating and disengaging under the spring restoring force of the fourth compression spring 64; the second copper sleeve or the second linear bearing 66 can make the lateral sliding sleeve 12 slide along the outer side of the lateral sliding rod 9 smoother, more stable and more wear-resistant. In order to improve the lubrication effect, the second copper sleeve can use a graphite self-lubricating copper sleeve; the lateral anti-detachment block 63 can prevent the lateral tool rod 59 from separating from the lateral tool rod sleeve 58 under the spring restoring force of the third compression spring 62. The lateral anti-detachment block 63 can be installed on the lateral tool rod 59 by means of threads or threaded fasteners in the existing known technology.

[0049] Embodiment 8: As attached Figure 1 , 2 As shown in Figure 3, a support assembly is slidably installed on the outer side of the right part of the transverse slide bar 9, and the support assembly includes a support sleeve 33, a fixing bolt 34, an inner sleeve rod 35, a fifth compression spring 36, an outer sleeve 37, a flexible foot seat 38 and a locking bolt 39. The outer side of the right part of the transverse slide bar 9 is sleeved with a support sleeve 33, and a fixing screw hole that passes through the inside and outside of the support sleeve 33 is provided on the upper side of the support sleeve 33. A fixing bolt 34 whose inner end abuts against the outer side of the transverse slide bar 9 is provided in the fixing screw hole; an inner sleeve is fixedly installed on the lower side of the support sleeve 33. Rod 35, an outer sleeve 37 is sleeved on the outer side of the inner sleeve rod 35, a flexible foot 38 whose lower end abuts against the upper side of the base 3 is fixedly installed on the lower side of the outer sleeve 37, and a fifth compression spring 36 is provided in the outer sleeve 37 corresponding to the position between the flexible foot 38 and the inner sleeve rod 35; a locking screw hole that passes through inside and outside is provided on the right side of the upper part of the outer sleeve 37, and an anti-slip groove 48 with an opening facing outward is provided on the inner sleeve rod 35 corresponding to the position of the locking screw hole, and a locking bolt 39 is provided in the locking screw hole with the inner end and the anti-slip groove 48.

[0050] By such arrangement, the inner end of the locking bolt 39 rests against the bottom of the anti-slip groove 48 to fix the relative position between the inner sleeve rod 35 and the outer sleeve 37; the inner sleeve rod 35, the outer sleeve 37 and the flexible foot 38 can temporarily fix the PVDF film when forming a support to prevent the PVDF film from moving during the cutting process, and can also prevent the transverse slide bar 9 or the longitudinal slide bar 11 from deforming during the sliding process of the transverse cutting component and the longitudinal cutting component to affect the cutting accuracy; the fifth compression spring 36 can make the flexible foot 38 automatically extend downward to improve the adjustment efficiency; in addition, when the base 3 is made of ferromagnetic material, the flexible foot 38 can also be realized by a magnet fixedly installed on the lower side of the outer sleeve 37 to form a stable support through magnetic force.

[0051] Embodiment 9: As attached Figure 1 , 2 As shown, the lower right rear side of the upper handle 1 and the upper right rear side of the lower handle 2 are hinged together through a hinge shaft 5, and a torsion spring 40 is provided on the outer side of the hinge shaft 5 corresponding to the position between the upper handle 1 and the lower handle 2. The rear end and the right end of the L-shaped shearing knife 4 can be detachably installed with a first baffle 41, and the right end of the transverse slide bar 9 can be detachably installed with a second baffle 42. Through such an arrangement, the L-shaped shearing knife 4 can be automatically closed and a downward shearing force can be applied to the L-shaped shearing knife 4, so as to automatically cut the PVDF film, improve the cutting efficiency, and save more effort; the torsion spring 40 can also make the lower end of the transverse support assembly and the lower end of the longitudinal support assembly always abut against the upper side of the base 3 to prevent the L-shaped shearing knife 4 from shaking; the first baffle 41 is used to prevent the longitudinal slide bar 15 from detaching from the L-shaped shearing knife 4, and the second baffle 42 is used to prevent the support assembly from detaching from the transverse slide bar 9. The detachable installation of the first baffle 41 and the second baffle 42 can be achieved by threaded fasteners.

[0052] Embodiment 10: As attached Figure 1 , 2 As shown, a coordinate grid 43 is provided on the upper side of the base 3 corresponding to the position between the first shearing groove 6 and the second shearing groove 7, a longitudinal scale 44 is provided on the upper side of the longitudinal slide bar 11 corresponding to the position of the coordinate grid 43, a transverse scale 45 is provided on the upper side of the transverse slide bar 9 corresponding to the position of the coordinate grid 43, and two mutually perpendicular shearing scales 42 are provided on the upper side of the L-shaped shearing knife 4, and the zero points of the two shearing scales 42 are located at the corner positions of the L-shaped shearing knife 4.

[0053] Through such an arrangement, the coordinate grid 43 is more convenient for positioning the predetermined target area 49, and is easy to quickly adjust the relative position between the transverse cutting component and the longitudinal cutting component and the predetermined target area 49; the size of the target cutting area can be quickly and accurately adjusted through the longitudinal scale 44, the transverse scale 45 and the shear scale 42.

[0054] The above technical features constitute the embodiments of the present invention, which have strong adaptability and implementation effect. Non-essential technical features can be added or reduced according to actual needs to meet the requirements of different situations.

Claims

1. A quick pair of scissors for cutting PVDF membrane before and after Western blotting, characterized in that It includes an upper handle and a lower handle which are arranged at an upper and lower interval, a base, and an L-shaped cutting knife. The lower side of the right rear part of the upper handle and the upper side of the right rear part of the lower handle are hinged together. The right rear end of the lower handle is fixedly installed on the outer side of the lower left corner of the base. An upward-opening first cutting groove is provided on the upper side of the front end of the base, and a second cutting groove which is upward-opening and whose front end communicates with the left end of the first cutting groove is provided on the upper side of the left end of the base. The right rear end of the upper handle is fixedly installed on the outer side of the corner of the L-shaped cutting knife. The lower end of the front part of the L-shaped cutting knife is located in the first cutting groove, and the lower end of the left part of the L-shaped cutting knife is located in the second cutting groove. A transverse support assembly which can make the L-shaped cutting knife open at a certain angle is slidably installed on the L-shaped cutting knife corresponding to the position above the second cutting groove. A transverse sliding rod is detachably installed on the upper part of the transverse support assembly. A through guide chute is provided on the transverse sliding rod corresponding to the inner side of the left part of the L-shaped cutting knife. A longitudinal support assembly which can make the L-shaped cutting knife open at a certain angle is slidably installed on the L-shaped cutting knife corresponding to the position above the first cutting groove. A longitudinal sliding rod whose rear part is located in the guide chute is detachably installed on the upper part of the longitudinal support assembly. A first longitudinal cutting assembly which can cut the PVDF film by sliding back and forth along the longitudinal sliding rod is sleeved on the outer side of the longitudinal sliding rod corresponding to the position between the transverse sliding rod and the front part of the L-shaped cutting knife. A first transverse cutting assembly which can cut the PVDF film by sliding left and right along the transverse sliding rod is sleeved on the outer side of the transverse sliding rod corresponding to the position between the longitudinal sliding rod and the left part of the L-shaped cutting knife. The cutting path of the L-shaped cutting knife, the cutting path of the first longitudinal cutting assembly, and the cutting path of the first transverse cutting assembly form a target cutting area which can cut the predetermined target area on the PVDF film at one time.

2. The quick scissors for cutting PVDF membrane before and after Western blotting according to claim 1, characterized in that The longitudinal support assembly includes a longitudinal vertical shaft seat, a longitudinal fastening bolt, a longitudinal slider, and a longitudinal adjusting bolt. A longitudinally convex-shaped longitudinal vertical shaft seat is provided on the outer side of the front end of the longitudinal sliding rod. A longitudinally through fastening screw hole is provided in the middle of the upper side of the longitudinal vertical shaft seat. A longitudinal fastening bolt whose inner end abuts against the outer side of the longitudinal sliding rod is provided in the longitudinally fastening screw hole. A U-shaped longitudinal slider with a downward opening is provided between the lower side of the longitudinal vertical shaft seat and the outer side of the upper part of the L-shaped cutting knife. A longitudinal support platform is fixedly installed on the lower part of the front side of the longitudinal slider. A longitudinally through adjusting screw hole is provided on the longitudinal support platform. A longitudinal adjusting bolt whose lower end abuts against the upper side of the base is provided in the longitudinally adjusting screw hole. A first chute with a forward opening is provided on the front side of the L-shaped cutting knife corresponding to the lower part of the longitudinal slider. A second chute with a backward opening is provided on the rear side of the L-shaped cutting knife corresponding to the position of the first chute. First guide blocks whose outer ends are fixedly installed at corresponding positions on the inner side of the longitudinal slider are provided in both the second chute and the first chute. The cam is provided with a plurality of holes for holding the guide rails together so that the guide rails can be fixedly mounted on the support frame, and the holes can be adjusted accordingly.

3. The quick scissors for cutting PVDF membrane before and after Western blotting according to claim 1 or 2, characterized in that The longitudinal slide bar is a cylindrical slide bar, and the longitudinal cutting assembly includes a longitudinal sliding sleeve, a longitudinal adjustment sleeve, a longitudinal adjustment rod, a longitudinal locking bolt, a longitudinal knife rod sleeve, a longitudinal knife rod, a longitudinal knife seat and a longitudinal blade. The longitudinal sliding sleeve is sleeved on the outer side of the longitudinal slide bar, and the longitudinal adjustment sleeve is fixedly installed on the left side of the longitudinal sliding sleeve, and the longitudinal adjustment rod is sleeved in the longitudinal adjustment sleeve. The upper side of the left end of the longitudinal adjustment sleeve is provided with a longitudinal locking screw hole that passes through inside and outside, and the longitudinal locking screw hole is provided with a longitudinal locking bolt whose inner end abuts against the upper side of the longitudinal adjustment rod. The left end of the longitudinal adjustment rod is fixedly installed with a longitudinal knife rod sleeve whose left end is located on the right side of the L-shaped shearing knife, and the middle part of the longitudinal knife rod sleeve is provided with a longitudinal guide knife rod hole that passes through from top to bottom, and the longitudinal guide knife rod hole is sleeved with the longitudinal knife rod, and the lower end of the longitudinal knife rod is fixedly installed with the longitudinal knife seat, and the lower side of the longitudinal knife seat is provided with a longitudinal blade groove that opens downward and passes through front and back, and the longitudinal blade whose lower end abuts against the upper side of the base is installed in the longitudinal blade groove; Or / and, the transverse sliding rod is a cylindrical sliding rod, the transverse cutting assembly includes a transverse sliding sleeve, a transverse adjustment sleeve, a transverse adjustment rod, a transverse locking bolt, a transverse tool rod sleeve, a transverse tool rod, a transverse tool seat and a transverse blade. A transverse sliding sleeve is mounted on the outer side of the transverse sliding rod, and a transverse adjustment sleeve is fixedly installed on the front side of the transverse sliding sleeve, and a transverse adjustment rod is mounted inside the transverse adjustment sleeve. A transverse locking screw hole that passes through inside and outside is provided on the upper side of the transverse adjustment sleeve, and a transverse locking bolt whose inner end abuts against the upper side of the transverse adjustment rod is provided in the transverse locking screw hole. A transverse tool rod sleeve whose front end is located behind the L-shaped shearing knife is fixedly installed on the front end of the transverse adjustment rod, a guide transverse tool rod hole that passes through up and down is provided in the middle part of the transverse tool rod sleeve, and a transverse tool rod is mounted in the guide transverse tool rod hole, and a transverse tool seat is fixedly installed on the lower end of the transverse tool rod, and a transverse blade groove that opens downward and passes through left and right is provided on the lower side of the transverse tool seat, and a transverse blade groove whose lower end abuts against the upper side of the base is installed.

4. The quick scissors for cutting PVDF membrane before and after Western blotting according to claim 3, characterized in that The longitudinal cutting assembly also includes a first compression spring, a longitudinal anti-slip block and a second compression spring. The first compression spring is provided on the outer side of the longitudinal knife rod corresponding to the position between the lower side of the longitudinal knife rod sleeve and the upper side of the longitudinal knife seat, and the longitudinal anti-slip block is detachably installed on the upper end of the longitudinal knife rod; a first waist-shaped groove opening upward is provided on the longitudinal adjustment rod corresponding to the position of the longitudinal locking bolt, and the lower end of the longitudinal locking bolt is located in the first waist-shaped groove, and a second compression spring is provided in the longitudinal adjustment sleeve corresponding to the right side position of the longitudinal adjustment rod, and a first copper sleeve or a first linear bearing is provided between the longitudinal sliding sleeve and the longitudinal sliding rod; Or / and, the transverse cutting assembly also includes a third compression spring, a transverse anti-slip block and a fourth compression spring. A third compression spring is provided on the outer side of the transverse knife rod corresponding to the position between the lower side of the transverse knife rod sleeve and the upper side of the transverse knife seat, and a transverse anti-slip block is detachably installed on the upper end of the transverse knife rod; a second waist-shaped groove opening upward is provided on the transverse adjustment rod corresponding to the position of the transverse locking bolt, and the lower end of the transverse locking bolt is located in the second waist-shaped groove, and a fourth compression spring is provided in the transverse adjustment sleeve corresponding to the rear side of the transverse adjustment rod, and a second copper sleeve or a second linear bearing is provided between the inner side of the transverse sliding sleeve and the outer side of the transverse sliding rod.

5. The quick scissors for cutting the PVDF membrane before and after Western blotting according to claim 1, 2 or 4, characterized in that A support assembly is slidably installed on the outer side of the right part of the transverse slide bar, and the support assembly includes a support sleeve, a tightening bolt, an inner sleeve rod, a fifth compression spring, an outer sleeve, a flexible foot and a locking bolt. A support sleeve is installed on the outer side of the right part of the transverse slide bar, and a tightening screw hole that passes through inside and outside is provided on the upper side of the support sleeve, and a tightening bolt whose inner end abuts against the outer side of the transverse slide bar is provided in the tightening screw hole; an inner sleeve rod is fixedly installed on the lower side of the support sleeve, and an outer sleeve is installed on the outer side of the inner sleeve rod, and a flexible foot whose lower end abuts against the upper side of the base is fixedly installed on the lower side of the outer sleeve, and a fifth compression spring is provided in the outer sleeve corresponding to the position between the flexible foot and the inner sleeve rod; a locking screw hole that passes through inside and outside is provided on the right side of the upper part of the outer sleeve, and an anti-slip groove that opens outward and is waist-shaped is provided on the inner sleeve rod corresponding to the position of the locking screw hole, and a locking bolt with the inner end and the anti-slip groove is provided in the locking screw hole.

6. The quick scissors for cutting PVDF membrane before and after Western blotting according to claim 3, characterized in that A support assembly is slidably installed on the outer side of the right part of the transverse slide bar, and the support assembly includes a support sleeve, a tightening bolt, an inner sleeve rod, a fifth compression spring, an outer sleeve, a flexible foot and a locking bolt. A support sleeve is installed on the outer side of the right part of the transverse slide bar, and a tightening screw hole that passes through inside and outside is provided on the upper side of the support sleeve, and a tightening bolt whose inner end abuts against the outer side of the transverse slide bar is provided in the tightening screw hole; an inner sleeve rod is fixedly installed on the lower side of the support sleeve, and an outer sleeve is installed on the outer side of the inner sleeve rod, and a flexible foot whose lower end abuts against the upper side of the base is fixedly installed on the lower side of the outer sleeve, and a fifth compression spring is provided in the outer sleeve corresponding to the position between the flexible foot and the inner sleeve rod; a locking screw hole that passes through inside and outside is provided on the right side of the upper part of the outer sleeve, and an anti-slip groove that opens outward and is waist-shaped is provided on the inner sleeve rod corresponding to the position of the locking screw hole, and a locking bolt with the inner end and the anti-slip groove is provided in the locking screw hole.

7. The quick scissors for cutting the PVDF membrane before and after Western blotting according to claim 1, 2, 4 or 6, characterized in that The lower side of the right rear portion of the upper handle and the upper side of the right rear portion of the lower handle are hinged together by a hinge shaft, a torsion spring is provided on the outer side of the hinge shaft corresponding to the position between the upper handle and the lower handle, the rear end and the right end of the L-shaped shearing knife are both detachably installed with a first baffle, and the right end of the transverse sliding rod is detachably installed with a second baffle; or / and, a coordinate grid is provided on the upper side of the base corresponding to the position between the first shearing groove and the second shearing groove, a longitudinal scale is provided on the upper side of the longitudinal sliding rod corresponding to the position of the coordinate grid, a transverse scale is provided on the upper side of the transverse sliding rod corresponding to the position of the coordinate grid, and two mutually perpendicular shearing scales are provided on the upper side of the L-shaped shearing knife, and the zero points of the two shearing scales are located at the corner positions of the L-shaped shearing knife.

8. The quick scissors for cutting PVDF membrane before and after Western blotting according to claim 3, characterized in that The lower side of the right rear portion of the upper handle and the upper side of the right rear portion of the lower handle are hinged together by a hinge shaft, a torsion spring is provided on the outer side of the hinge shaft corresponding to the position between the upper handle and the lower handle, the rear end and the right end of the L-shaped shearing knife are both detachably installed with a first baffle, and the right end of the transverse sliding rod is detachably installed with a second baffle; or / and, a coordinate grid is provided on the upper side of the base corresponding to the position between the first shearing groove and the second shearing groove, a longitudinal scale is provided on the upper side of the longitudinal sliding rod corresponding to the position of the coordinate grid, a transverse scale is provided on the upper side of the transverse sliding rod corresponding to the position of the coordinate grid, and two mutually perpendicular shearing scales are provided on the upper side of the L-shaped shearing knife, and the zero points of the two shearing scales are located at the corner positions of the L-shaped shearing knife.

9. The quick scissors for cutting PVDF membrane before and after Western blotting according to claim 5, characterized in that The lower side of the right rear portion of the upper handle and the upper side of the right rear portion of the lower handle are hinged together by a hinge shaft, a torsion spring is provided on the outer side of the hinge shaft corresponding to the position between the upper handle and the lower handle, the rear end and the right end of the L-shaped shearing knife are both detachably installed with a first baffle, and the right end of the transverse sliding rod is detachably installed with a second baffle; or / and, a coordinate grid is provided on the upper side of the base corresponding to the position between the first shearing groove and the second shearing groove, a longitudinal scale is provided on the upper side of the longitudinal sliding rod corresponding to the position of the coordinate grid, a transverse scale is provided on the upper side of the transverse sliding rod corresponding to the position of the coordinate grid, and two mutually perpendicular shearing scales are provided on the upper side of the L-shaped shearing knife, and the zero points of the two shearing scales are located at the corner positions of the L-shaped shearing knife.

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