A feeding method for increasing the chondroitin sulfate content in chicken

Abstract

The present invention discloses a method for increasing the chondroitin sulfate content in chicken meat. By utilizing the metabolic and conversion effects of chondroitin sulfate in chickens, chondroitin sulfate is added to chicken feed at an addition amount of 0.25% - 2%, and the chickens are fed for 30 days. This can significantly increase the chondroitin sulfate content in chicken meat, obtain functional chicken meat rich in chondroitin sulfate, especially increase the chondroitin sulfate content in chicken breast and chicken leg meat, enhance the nutritional value of chicken meat, and also improve the economic value.

Description

Technical Field

[0001] The present invention belongs to the technical field of livestock and poultry product production in animal husbandry, and particularly relates to a method for increasing the content of chondroitin sulfate in chicken meat. Background Art

[0002] As an active polysaccharide in vivo, chondroitin sulfate participates in many important biological processes in body cells, such as regenerating cartilage, promoting the growth of osteoblasts, enabling bone injury to heal faster, and having an indirect protective effect on articular cartilage. Chondroitin sulfate also has various effects such as anti-inflammatory, anti-tumor, antioxidant, and improving the intestinal flora. The lack of chondroitin sulfate can cause arthritis-like diseases, such as hyperosteogeny and scapulohumeral periarthritis. Supplementing chondroitin sulfate can reduce the damage to articular cartilage.

[0003] Chicken meat is rich in nutrients such as protein and is a common meat for people to obtain nutrition. The content of chondroitin sulfate in chicken meat is relatively low, and the content of chondroitin sulfate in every kilogram of ordinary chicken meat is about 6 - 9 mg. In order to increase the additional nutrition in chicken meat, breeders can add some active ingredients beneficial to humans to the feed, and after metabolism and transformation in chickens, increase the enrichment of active substances in chicken meat.

[0004] At present, the main way for people to supplement chondroitin sulfate is to orally take chondroitin sulfate tablets or capsule products. Enriching chondroitin sulfate in chicken meat through biotransformation and supplementing chondroitin sulfate while eating chicken meat is more acceptable to people than taking tablets or capsules. There is no development of products with enriched chondroitin sulfate in animal-derived foods and no application of chondroitin sulfate in livestock and poultry breeding at home and abroad. In the early stage of the present invention, an acute toxicity test of chondroitin sulfate on chicks was carried out, and the results showed that the oral LD 50 > 30000 mg / (kg·bw) of chondroitin sulfate, which belongs to non-toxic substances.

[0005] The present invention relates to adding chondroitin sulfate to the diet to produce chicken meat rich in chondroitin sulfate. Summary of the Invention

[0006] The purpose of the present invention is to provide a method for increasing the content of chondroitin sulfate in chicken meat; this method can improve the nutritional value and added value of chicken meat, and is beneficial to improving the breeding economic benefits.

[0007] In order to achieve the above purpose, the present invention discloses the following technical solution:

[0008] A feeding method for increasing the content of chondroitin sulfate in chicken meat, characterized in that 0.25% - 2% of chondroitin sulfate is added to ordinary chicken feed.

[0009] The method for increasing the chondroitin sulfate content in chicken meat described above is: adding chondroitin sulfate to ordinary chicken feed. As a limitation: the addition amount of chondroitin sulfate in the ordinary chicken feed is 0.25% - 2% of the feed weight. As a further limitation: the feeding scheme is to feed caged chickens with ordinary feed containing 0.25% - 2% chondroitin sulfate for 30 days, with free access to food and water during the feeding process and conventional management.

[0010] The present invention further discloses the application of chicken meat obtained by the above feeding method in effectively increasing the chondroitin sulfate content in chicken meat. The experimental results show that: the main difference between the chicken meat rich in chondroitin sulfate obtained by the present invention and ordinary chicken meat is that its composition contains a higher chondroitin sulfate content. One of the experiments shows that: the chondroitin sulfate content in the chicken leg meat of the control group is 6.26 mg per kilogram, while the chondroitin sulfate content in the experimental group with 2% chondroitin sulfate added can reach 18.86 mg per kilogram, significantly higher than that of the control group (P < 0.05); the chondroitin sulfate content in the chicken breast meat of the control group is 8.96 mg per kilogram, while the chondroitin sulfate content in the experimental group with 2% chondroitin sulfate added can reach 34.25 mg per kilogram, significantly higher than that of the control group (P < 0.05).

[0011] The beneficial effect of the present invention in increasing the chondroitin sulfate content in chicken meat lies in: using the biological transformation effect of chondroitin sulfate in chickens to increase the chondroitin sulfate content in chicken meat, thereby improving the nutritional value and economic value of chicken meat.

[0012] The present invention focuses on investigating the promoting effect of chondroitin sulfate on the chondroitin sulfate content in chicken meat, mainly solving the method for increasing the chondroitin sulfate content in chicken meat. The difficulty of the invention lies in determining the specific application dosage of the feed additive that can increase the chondroitin sulfate content in chicken meat, which is the most important research content for increasing the chondroitin sulfate content in chicken meat. Detailed implementation mode

[0013] The present invention will be described below through specific implementation examples. Unless otherwise specified, the technical means used in the present invention are all methods well-known to those skilled in the art. In addition, the implementation examples should be understood as illustrative rather than limiting the scope of the present invention. The essence and scope of the present invention are only defined by the claims. For those skilled in the art, without departing from the essence and scope of the present invention, various changes or modifications to the material components and dosages in these implementation examples also fall within the protection scope of the present invention. The raw materials and reagents used in the present invention are all commercially available. For example, chondroitin sulfate is commercially available. The basal diet is composed of raw materials such as corn, soybean meal, peanut meal, soybean oil, calcium hydrogen phosphate, stone powder, sodium chloride, amino acids, vitamins, mineral elements, phytase, compound enzyme preparations, etc., and is commercially available.

[0014] Examples 1 - 4

[0015] Method for increasing chondroitin sulfate content in chicken

[0016] Examples 1 - 4 are respectively methods for increasing chondroitin sulfate content in chicken. The method is to add chondroitin sulfate to ordinary chicken feed, and the difference lies in the addition amount of chondroitin sulfate in each example (see Table 1).

[0017] Table 1 Addition amounts of chondroitin sulfate in feeds of Examples 1 - 4

[0018]

[0019] Example 5

[0020] Verification test on method for increasing chondroitin sulfate content in chicken

[0021] A cage - layer chicken breeding base in Tianjin

[0022] (1) Experimental animals and grouping

[0023] Select 210 healthy North China Chai chickens at 70 weeks of age, randomly divided into 1 control group and 4 experimental groups, with 3 replicates in each group and 14 laying hens in each replicate.

[0024] (2) Diet and feeding management

[0025] The control group was fed a basal diet (Table 2); experimental groups 1, 2, 3, and 4 were respectively added with 0.25%, 0.5%, 1%, and 2% of chondroitin sulfate to the basal diet; during the experiment, chickens in each group were allowed to eat and drink freely; the experimental period was 30 days.

[0026] Table 2 Composition of basal diet

[0027]

[0028] Composition of raw materials of basal diet: corn, soybean meal, peanut meal, soybean oil, calcium hydrogen phosphate, stone powder, sodium chloride, amino acids, vitamins, mineral elements, phytase, compound enzyme preparation, etc.

[0029] (3) Sampling and index determination of samples

[0030] On the 30th day of the experiment, 10 chickens in each group were dissected, and leg meat and breast meat were collected for detecting the chondroitin sulfate content in the meat. The determination of chondroitin sulfate content in chicken was carried out by high - performance liquid chromatography (HPLC) method.

[0031] The sample extraction method (cetylpyridinium chloride method) is as follows: Defat the chicken breast (leg) minced meat twice with ethanol; Dry the defatted meat at 60 °C and grind it into powder; Weigh 10 g of the meat powder, add 50 mmol / L sodium carbonate solution according to the solid-liquid ratio of 1:4, react at 55 °C for 4 h, and stir gently every half hour; Inactivate the enzyme at 100 °C for 10 min and cool to 4 °C; Centrifuge at 8000 r / min for 20 min and collect the supernatant; Add 5% trichloroacetic acid according to the mass of the filtrate and let it stand for 3 h, centrifuge at 5000 r / min for 5 min and collect the supernatant; Precipitate with 3-fold ethanol at 4 °C overnight; Centrifuge to collect the precipitate, dissolve the precipitate in 20 mmol / L sodium sulfate solution, centrifuge at 5000 r / min for 5 min, and collect the supernatant; Slowly add a solution of 6% cetylpyridinium chloride by mass concentration to the supernatant until no more precipitate forms; Centrifuge to collect the precipitate, redissolve the precipitate in 2M sodium chloride-ethanol (100:15) aqueous solution, precipitate with 3-fold ethanol at 4 °C overnight; Centrifuge to obtain the precipitate, dissolve it in distilled water, dialyze for 24 h, concentrate, and freeze-dry to obtain solid chondroitin sulfate.

[0032] The sample pretreatment method is as follows: Dissolve the solid chondroitin sulfate sufficiently with 900 μL of Tris-acetic acid buffer, add 100 μL of chondroitin ABC enzyme solution, mix well, place it in a 37 °C water bath for 1 h, inactivate the enzyme in a 100 °C water bath for 5 min, cool to room temperature, centrifuge at 10000 r / min for 20 min, take the supernatant, and filter it through a 0.22 μm filter membrane.

[0033] The chromatographic conditions for sample determination are as follows: Liquid chromatography column: Agilent ZORBAX SAX; Mobile phase: Mobile phase A: water, Mobile phase B: 2 mol / L sodium chloride solution; Flow rate: 1.0 mL / min; Detector: Ultraviolet-visible light detector; Column temperature: 40 °C; Detection wavelength: 232 nm; Injection volume: 20 μL; Elution method: Gradient elution is carried out according to Table 3.

[0034] Table 3 Gradient elution table for enzymatic hydrolysis liquid phase method

[0035]

[0036] (4)Test results

[0037] The test results are shown in Table 4. The test results show that the content of chondroitin sulfate in the chicken breast of each test group increased significantly compared with the control group (P<0.05), and the test group 4 was 2.1 times that of the control group; The content of chondroitin sulfate in the chicken leg of each test group increased significantly compared with the control group (P<0.05), and the test group 4 was 5.47 times that of the control group; As the addition dose of chondroitin sulfate in the diet increased, the content of chondroitin sulfate in the chicken breast and chicken leg of the test group increased.

[0038] Table 4 Content of chondroitin sulfate in chicken (mg / kg)

[0039]

[0040] Note: The same superscript letters indicate no significant difference (P > 0.05), while different superscript letters indicate significant difference (P ≤ 0.05).

[0041] Conclusion: The present invention can effectively increase the content of chondroitin sulfate in chicken and produce functional chicken rich in chondroitin sulfate.

Claims

1. A feeding method for increasing the chondroitin sulfate content in chicken meat, characterized in that Add chondroitin sulfate feed at 0.25% - 2% to the ordinary feed, and the feeding time is 30 days; the chicken meat mentioned refers to: chicken breast meat and chicken leg meat.

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