Anti-inflammatory and antibacterial oral gel and its preparation method and application

Abstract

The present application relates to the field of oral gels, and specifically discloses an anti-inflammatory and antibacterial oral gel, its preparation method, and application. The anti-inflammatory and antibacterial oral gel of the present application is prepared from the following raw materials in parts by weight: 4-6 parts of chitosan / fucoidan / beclomethasone dipropionate microcapsules, 20-30 parts of sodium alginate, 20-30 parts of chitosan, 0.2-0.5 parts of photoinitiator, and 20,000 parts of water; wherein the chitosan / fucoidan / beclomethasone dipropionate microcapsules are prepared from chitosan, fucoidan, beclomethasone dipropionate, calcium chloride, glycerol, and water. The anti-inflammatory and antibacterial oral gel of the present application, while sterilizing and anti-inflammatory, further enables the sustained release of the drug efficacy in the chitosan / fucoidan / beclomethasone dipropionate microcapsules, thereby prolonging the sterilization and anti-inflammatory time.

Description

Technical Field

[0001] The present invention relates to the field of oral gels, and more particularly to an anti-inflammatory and antibacterial oral gel and a preparation method and application thereof. Background Art

[0002] Oral gel works by continuously adhering to the oral wound or swollen surface, blocking the erosion of oral saliva and oral flora on the oral wound or swollen surface, thereby repairing and promoting wound healing.

[0003] In related art, Chinese application document CN202211304330.0 discloses an oral care gel comprising the following raw materials in weight percentage: 2-5% Morinda officinalis polysaccharide extract, 0.5-1% gel, and 1-3% ornidazole. The preparation method is as follows: 1) Reflux extract Morinda officinalis with ethanol for 2-3 times, filter, and dry the residue for later use; 2) Hot water extraction: Reflux extract the residue obtained in step 1) with 80-90°C water for 2-3 times. After the extraction, filter and centrifuge to obtain the supernatant, which is the Morinda officinalis polysaccharide crude extract; 3) Concentrate the crude polysaccharide extract obtained in step 2) under reduced pressure to form a viscous extract, then add ethanol to adjust the alcohol content to 60-70%, mix well, precipitate at 4-5°C for 12-14 hours, centrifuge, collect the precipitate, and freeze-dry to obtain Morinda officinalis polysaccharide. The Morinda officinalis polysaccharide extract, gel, ornidazole, and water are uniformly mixed according to the ratio and packaged to obtain the oral care gel.

[0004] Regarding the above-mentioned related technologies, the above-mentioned oral care gel is prepared by directly mixing raw materials. The polysaccharide extract of Morinda officinalis and ornidazole will directly release the efficacy, so that the oral care gel has better efficacy in a short period of time; but the efficacy is maintained for a short time. Summary of the Invention

[0005] In order to prolong the time that the oral gel exerts its anti-inflammatory and repairing effects on the oral cavity, the present application provides an anti-inflammatory and antibacterial oral gel, a preparation method and application thereof.

[0006] In a first aspect, the present application provides an anti-inflammatory and antibacterial oral gel, which adopts the following technical solution:

[0007] An anti-inflammatory and antibacterial oral gel is prepared from the following raw materials in parts by weight: 4-6 parts of chitosan / fucoidan / beclomethasone dipropionate microcapsules, 20-30 parts of sodium alginate, 20-30 parts of chitosan, 0.2-0.5 parts of a photoinitiator and 20,000 parts of water.

[0008] By adopting the above technical solution, fucoidan has multiple immune activities, mainly including anti-complement activity, anti-inflammatory response and immunomodulatory effects. Beclomethasone dipropionate has anti-inflammatory, anti-allergic and antipruritic effects, a strong vasoconstrictive effect on the skin, strong lipophilicity and easy penetration. Due to the strong efficacy of beclomethasone dipropionate, chitosan / fucoidan / beclomethasone dipropionate microcapsules are prepared to achieve sustained release of beclomethasone dipropionate.

[0009] Since chitosan itself has bactericidal and anti-inflammatory effects, a large amount of chitosan forms a hydrogel together with sodium alginate to wrap the chitosan / fucoidan / beclomethasone dipropionate microcapsules. While killing bacteria and fighting inflammation, it further enables the sustained release of the drug efficacy in the chitosan / fucoidan / beclomethasone dipropionate microcapsules, thereby prolonging the bactericidal and anti-inflammatory time.

[0010] Optionally, the chitosan / fucoidan / beclomethasone dipropionate microcapsules are prepared from chitosan, fucoidan, beclomethasone dipropionate, calcium chloride, glycerol and water.

[0011] By adopting the above technical solution, chitosan can be used as the coating material for fucoidan and beclomethasone dipropionate, so that the fucoidan and beclomethasone dipropionate that exert the main pharmacological effects are coated in the chitosan, and released layer by layer through the chitosan coating layer and the chitosan and sodium alginate outer coating, thereby achieving a sustained-release effect; calcium chloride can promote the formation of the chitosan coating material and accelerate the molding speed and molding effect of the chitosan / fucoidan / beclomethasone dipropionate microcapsules.

[0012] Optionally, the chitosan / fucoidan / beclomethasone dipropionate microcapsules are prepared from 2-4 parts of chitosan, 3-5 parts of fucoidan, 1-3 parts of beclomethasone dipropionate, 0.2-0.5 parts of calcium chloride, 5-8 parts of glycerol, and 5-10 parts of water. The above raw materials are based on the chitosan / fucoidan / beclomethasone dipropionate microcapsules.

[0013] By adopting the above technical solution, the chitosan / fucoidan / beclomethasone dipropionate microcapsules prepared from the above-mentioned weight portions of chitosan, fucoidan, beclomethasone dipropionate, calcium chloride, glycerol and water can not only achieve sustained release of fucoidan and beclomethasone dipropionate in the chitosan / fucoidan / beclomethasone dipropionate microcapsules, but also exert their medicinal effects throughout the entire sustained release process.

[0014] Optionally, the preparation steps of the chitosan / fucoidan / beclomethasone dipropionate microcapsules are as follows: mixing fucoidan, beclomethasone dipropionate and glycerol to obtain a sustained-release solution; mixing chitosan, calcium chloride and water to obtain a coating; adding the coating to the sustained-release solution and stirring to obtain chitosan / fucoidan / beclomethasone dipropionate microcapsules.

[0015] By adopting the above technical solution, in the above preparation method, the sustained-release solution and the coating are prepared separately, so that before the chitosan is mixed with the sustained-release solution, it is cross-linked into macromolecular chitosan under the action of calcium chloride, which is the coating in this application; when the coating is further mixed with the sustained-release solution, the coating has a better coating effect on the sustained-release solution and a faster coating speed.

[0016] Optionally, the coating is added to a sustained-release solution, stirred at a speed of 800 rpm-1000 rpm for 5-10 minutes, and kept at a constant temperature of 80-90° C. to form a mixed phase; the mixed phase is then pre-emulsified at a speed of 800 rpm-1000 rpm and an emulsification time of 30-40 minutes to form a mixed phase emulsion; the mixed phase emulsion is pressurized to 500-600 bar, and the cycle is repeated 3-5 times to obtain chitosan / fucoidan / beclomethasone dipropionate microcapsules.

[0017] By adopting the above technical solution, the high-temperature stirring process allows the coating and the sustained-release solution to be fully dispersed; the purpose of emulsification is to further mix the water-soluble coating and the oil-soluble sustained-release solution evenly to form an emulsion; under pressurized conditions, chitosan / fucoidan / beclomethasone dipropionate microcapsules are finally produced.

[0018] Optionally, the photoinitiator is isopropylthioxanthone.

[0019] By adopting the above technical solution, isopropylthioxanthone is non-toxic and odorless, and is harmless to human skin when in contact with human skin; in this application, it is used as a photoinitiator to promote the formation of a hydrogel cross-linked network between chitosan and sodium hydroxide; the anti-inflammatory and antibacterial oral gel finally obtained is highly safe.

[0020] In a second aspect, the present application provides a method for preparing an anti-inflammatory and antibacterial oral gel, which adopts the following technical scheme: a method for preparing an anti-inflammatory and antibacterial oral gel, comprising the following steps: dissolving chitosan and sodium alginate in water, stirring thoroughly to obtain a uniform chitosan aqueous solution, dialyzing, and freeze-drying; then adding chitosan / fucoidan / beclomethasone dipropionate microcapsules and a photoinitiator, stirring evenly to obtain a mixed solution, and irradiating the mixed solution under ultraviolet light to obtain the anti-inflammatory and antibacterial oral gel.

[0021] By adopting the above technical solution, the dialyzed chitosan and sodium alginate both exist in the form of small molecules in water. When mixed with the chitosan / fucoidan / beclomethasone dipropionate microcapsules, an oral antibacterial gel with uniform hardness is formed, and the chitosan / fucoidan / beclomethasone dipropionate microcapsules have a relatively good drug release effect. Under the irradiation of ultraviolet light, through the initiation effect of the photoinitiator, the chitosan and sodium alginate quickly and densely coat the chitosan / fucoidan / beclomethasone dipropionate microcapsules, forming an oral antibacterial gel with a good cross-linking effect.

[0022] Optionally, the chitosan and sodium alginate are dissolved in water and the pH is adjusted to 7-9.

[0023] By adopting the above technical solution, the pH is adjusted to 7-9, so that the aqueous solution of chitosan and sodium alginate becomes an alkaline aqueous solution. Since both chitosan and sodium alginate carry a large number of hydroxyl groups, they can exist stably in alkaline water. In the final oral antibacterial gel, the distribution of chitosan and sodium alginate is highly uniform, and the bactericidal and anti-inflammatory effects are good.

[0024] In a third aspect, the present application provides an application of an anti-inflammatory and antibacterial oral gel, which adopts the following technical solution:

[0025] The invention discloses an anti-inflammatory and antibacterial oral gel for use in treating oral gingival swelling and oral ulcers.

[0026] By adopting the above technical solution, the anti-inflammatory and antibacterial oral gel of the present application can be anti-inflammatory and bactericidal, and has good cell compatibility and is non-toxic; it is suitable for use on the gums after tooth extraction, swollen gums and ulcer surfaces in the oral cavity.

[0027] In summary, this application has the following beneficial effects:

[0028] 1. In the present application, beclomethasone dipropionate has anti-inflammatory, anti-allergic and antipruritic effects. However, due to the strong efficacy of beclomethasone dipropionate, chitosan / fucoidan / beclomethasone dipropionate microcapsules are prepared to achieve sustained release of beclomethasone dipropionate. A large amount of chitosan forms a hydrogel together with sodium alginate to encapsulate the chitosan / fucoidan / beclomethasone dipropionate microcapsules. While achieving bactericidal and anti-inflammatory effects, the chitosan / fucoidan / beclomethasone dipropionate microcapsules further achieve sustained release of the drug efficacy, thereby prolonging the bactericidal and anti-inflammatory time.

[0029] 2. In the preparation method, the sustained-release solution and the coating are prepared separately, so that before the chitosan is mixed with the sustained-release solution, it is cross-linked into a large molecular chitosan under the action of calcium chloride, which is the coating in this application; when the coating is further mixed with the sustained-release solution, the coating has a better coating effect on the sustained-release solution;

[0030] 3. In the preparation method of the oral antibacterial gel, under the irradiation of ultraviolet light, the chitosan and sodium alginate quickly coat the chitosan / fucoidan / beclomethasone dipropionate microcapsules through the initiation effect of the photoinitiator, and form an oral antibacterial gel with good cross-linking effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The purpose is to show the cumulative release of methamphetamine propionate over time of the anti-inflammatory and antibacterial oral gel prepared in Example 3 of the present application. DETAILED DESCRIPTION

[0032] The present application is further described in detail below with reference to the following examples and comparative examples.

[0033] The sources of raw materials for the following examples and comparative examples are provided: the raw materials for the examples and comparative examples are all commercially available, and 2-isopropylthioxanthone and isopropylthioxanthone (a mixture of 2- and 4-isomers) were purchased from Dafeng Tiansheng Pharmaceutical Co., Ltd.

[0034] Preparation Example of Chitosan / Fucoidan / Beclomethasone Dipropionate Microcapsules

[0035] Preparation Example 1

[0036] 5 g of fucoidan, 1 g of beclomethasone dipropionate and 5 g of glycerol were mixed at 800 rpm for 5 min to obtain a sustained-release solution; 2 g of chitosan, 0.5 g of calcium chloride and 10 g of water were mixed at 1000 rpm for 10 min to obtain a coating;

[0037] The coated material was added to the sustained-release solution, stirred at 800 rpm for 8 minutes, and kept at a constant temperature of 85°C to form a mixed phase; the mixed phase was pre-emulsified using a homogenizer at a speed of 1000 rpm and an emulsification time of 35 minutes to form a mixed phase emulsion; the mixed phase emulsion was pressurized to 550 bar and recycled four times to obtain chitosan / fucoidan / beclomethasone dipropionate microcapsules.

[0038] Preparation Example 2

[0039] 3 g of fucoidan, 3 g of beclomethasone dipropionate and 8 g of glycerol were mixed at 800 rpm for 5 min to obtain a sustained-release solution; 4 g of chitosan, 0.2 g of calcium chloride and 5 g of water were mixed at 1000 rpm for 10 min to obtain a coating;

[0040] The coated material was added to the sustained-release solution, stirred at 800 rpm for 8 minutes, and kept at a constant temperature of 85°C to form a mixed phase; the mixed phase was pre-emulsified using a homogenizer at a speed of 1000 rpm and an emulsification time of 35 minutes to form a mixed phase emulsion; the mixed phase emulsion was pressurized to 550 bar and recycled four times to obtain chitosan / fucoidan / beclomethasone dipropionate microcapsules.

[0041] Preparation Example 3

[0042] 4 g of fucoidan, 2 g of beclomethasone dipropionate and 6 g of glycerol were mixed at 800 rpm for 5 min to obtain a sustained-release solution; 3 g of chitosan, 0.3 g of calcium chloride and 8 g of water were mixed at 1000 rpm for 10 min to obtain a coating;

[0043] The coated material was added to the sustained-release solution, stirred at 800 rpm for 8 minutes, and kept at a constant temperature of 85°C to form a mixed phase; the mixed phase was pre-emulsified using a homogenizer at a speed of 1000 rpm and an emulsification time of 35 minutes to form a mixed phase emulsion; the mixed phase emulsion was pressurized to 550 bar and recycled four times to obtain chitosan / fucoidan / beclomethasone dipropionate microcapsules.

[0044] Comparative Preparation Example 1

[0045] The difference from Preparation Example 3 is that beclomethasone dipropionate was not added.

[0046] Comparative Preparation Example 2

[0047] The difference from Preparation Example 3 is that no fucoidan was added.

[0048] Comparative Preparation Example 3

[0049] The difference from Preparation Example 3 is that beclomethasone dipropionate and fucoidan are not added.

[0050] Examples of anti-inflammatory and antibacterial oral gels

[0051] Example 1

[0052] An anti-inflammatory and antibacterial oral gel, the preparation steps of which are:

[0053] 20 g of chitosan and 30 g of sodium alginate were dissolved in 20,000 g of water and adjusted to pH 8 with phosphate buffer solution. The mixture was stirred at 600 rpm for 5 min to obtain a uniform chitosan aqueous solution, which was dialyzed using an 8KD dialysis bag for 24 h and freeze-dried to obtain a gel solution. 4 g of the chitosan / fucoidan / beclomethasone propionate microcapsules prepared in Preparation Example 1 and 0.5 g of the photoinitiator 2-isopropylthioxanthone were then added to the gel solution and stirred to obtain a mixed solution. The mixed solution was then placed under a 100 W ultraviolet lamp (ultraviolet intensity 4800 μW / cm 2 ) for 5 min to obtain the anti-inflammatory and antibacterial oral gel.

[0054] Example 2

[0055] An anti-inflammatory and antibacterial oral gel, the preparation steps of which are:

[0056] 30 g of chitosan and 20 g of sodium alginate were dissolved in 20,000 g of water and adjusted to pH 8 with phosphate buffer solution. The mixture was stirred at 600 rpm for 5 min to obtain a uniform chitosan aqueous solution, which was dialyzed using an 8KD dialysis bag for 24 h and freeze-dried to obtain a gel solution. 6 g of the chitosan / fucoidan / beclomethasone propionate microcapsules prepared in Preparation Example 2 and 0.2 g of the photoinitiator 2-isopropylthioxanthone were then added to the gel solution and stirred to obtain a mixed solution. The mixed solution was then placed under a 100 W ultraviolet lamp (ultraviolet intensity 4800 μW / cm 2 ) for 5 min to obtain the anti-inflammatory and antibacterial oral gel.

[0057] Example 3

[0058] An anti-inflammatory and antibacterial oral gel, the preparation steps of which are:

[0059] 25 g of chitosan and 25 g of sodium alginate were dissolved in 20,000 g of water and adjusted to pH 8 with phosphate buffer solution. The mixture was stirred at 600 rpm for 5 min to obtain a uniform chitosan aqueous solution, which was dialyzed using an 8KD dialysis bag for 24 h and freeze-dried to obtain a gel solution. 5 g of the chitosan / fucoidan / beclomethasone propionate microcapsules prepared in Preparation Example 3 and 0.35 g of the photoinitiator 2-isopropylthioxanthone were then added to the gel solution and stirred to obtain a mixed solution. The mixed solution was then placed under a 100 W ultraviolet lamp (ultraviolet intensity 4800 μW / cm 2 ) for 5 min to obtain the anti-inflammatory and antibacterial oral gel.

[0060] Example 4

[0061] The difference from Example 3 is that the initiator 2-isopropylthioxanthone is replaced by an equal weight of isopropylthioxanthone (a mixture of 2- and 4-isomers).

[0062] Comparative Example 1

[0063] The difference from Example 3 is that the chitosan / fucoidan / beclomethasone dipropionate microcapsules prepared in Comparative Preparation Example 1 were used.

[0064] Comparative Example 2

[0065] The difference from Example 3 is that the chitosan / fucoidan / beclomethasone dipropionate microcapsules prepared in Comparative Preparation Example 2 were used.

[0066] Comparative Example 3

[0067] The difference from Example 3 is that the chitosan / fucoidan / beclomethasone dipropionate microcapsules prepared in Comparative Preparation Example 3 were used.

[0068] Comparative Example 4

[0069] The difference from Example 3 is that the raw materials for preparing chitosan / fucoidan / beclomethasone dipropionate microcapsules are directly mixed; an anti-inflammatory and antibacterial oral gel, the specific preparation steps of which are:

[0070] 25g of chitosan and 25g of sodium alginate were dissolved in 20,000g of water and adjusted to pH 8 with phosphate buffer solution. The mixture was stirred at 600rpm for 5min to obtain a uniform chitosan aqueous solution, which was dialyzed using an 8KD dialysis bag for 24h and freeze-dried to obtain a gel solution. 0.35g of the photoinitiator 2-isopropylthioxanthone and 5g of a raw material mixture of chitosan / fucoidan / beclomethasone dipropionate microcapsules were then added to the gel solution, wherein the weight ratio of chitosan, fucoidan, beclomethasone dipropionate, calcium chloride, glycerol to water was 3:4:2:0.3:6:8. The mixture was stirred evenly to obtain a mixed solution. The mixed solution was then irradiated under a 100W ultraviolet lamp (ultraviolet intensity 4800μW / cm 2 ) for 5 min to obtain the anti-inflammatory and antibacterial oral gel.

[0071] Comparative Example 5

[0072] The difference from Example 3 is that 5 g of the chitosan / fucoidan / beclomethasone dipropionate microcapsules prepared in Preparation Example 3 in Example 3 are replaced by 10 g of the chitosan / fucoidan / beclomethasone dipropionate microcapsules prepared in Preparation Example 3.

[0073] Comparative Example 6

[0074] The difference from Example 3 is that the photoinitiator 2-isopropylthioxanthone is not added.

[0075] Performance testing

[0076] The anti-inflammatory and antibacterial oral gels prepared in Examples 1 to 4 and Comparative Examples 1 to 6 were used to perform performance tests to detect adhesion performance, cumulative release of methadone propionate, antibacterial properties and cytocompatibility. The test results are shown in Table 1. The anti-inflammatory and antibacterial oral gel of Example 3 was tested for cumulative release percentages of methadone propionate at 1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 7 h and 8 h. The test results are shown in the accompanying drawings of the specification. Figure 1 As shown;

[0077] Adhesion performance test: The anti-inflammatory and antibacterial oral gel was adhered between two smooth glass surfaces. A 40-50g weight was placed vertically on one of the smooth glass surfaces to test the load that the anti-inflammatory and antibacterial oral gel could withstand.

[0078] Cumulative release of methadone propionate: Place the anti-inflammatory and antibacterial oral gel in an 8KD dialysis bag, place the dialysis bag in water, and dialyze for 8 hours. Detect the content of methadone propionate in the water.

[0079] Antibacterial activity test: After 8 hours of testing, the killing rate of the anti-inflammatory and antibacterial oral gel against Escherichia coli and Staphylococcus aureus;

[0080] Cytocompatibility testing: Cell viability was determined using cell fluorescence staining and CCK8 assays. Double-labeling fluorescence staining of actin cytoskeleton (red) and cell nucleus (blue) was used to study the cell viability of cells on the anti-inflammatory and antibacterial oral gel after 5 days.

[0081] Table 1

[0082]

[0083] Combining Examples 1, 2, and 3, it can be seen that the amounts of raw materials used in preparing the anti-inflammatory and antibacterial oral gels in Examples 1, 2, and 3 are different, but the adhesion performance, 8-hour cumulative release of methotrexate, sterilization rate, and cell viability of the anti-inflammatory and anti-inflammatory oral gels prepared can all meet the requirements for bactericidal and anti-inflammatory effects on oral skin.

[0084] In combination with Examples 3 and 4, it can be seen that in Example 4, 2-isopropylthioxanthone is replaced with isopropylthioxanthone (mixture of 2 and 4 isomers), and the adhesion performance, 8-h cumulative release of methamphetamine propionate, sterilization rate and cell viability of the anti-inflammatory and antibacterial oral gel prepared can meet the bactericidal and anti-inflammatory requirements of the oral skin; it is proved that 2-isopropylthioxanthone and isopropylthioxanthone (mixture of 2 and 4 isomers) are not harmful to human skin.

[0085] Combining Example 3 with Comparative Examples 1, 2, and 3, it can be seen that the difference from Example 3 is that Comparative Example 1 does not add beclomethasone dipropionate; Comparative Example 2 does not add fucoidan; Comparative Example 3 does not add fucoidan and beclomethasone dipropionate; Comparative Examples 1 and 3 do not have an 8-hour cumulative release of beclomethasone dipropionate, while the 8-hour cumulative release of beclomethasone dipropionate in Comparative Example 2 reaches 57%, and the release rate is too fast. After long-term use, it is easy to cause an impact on the human body; therefore, the anti-inflammatory and antibacterial oral gels prepared in Comparative Examples 1, 2, and 3 are not suitable for long-term use by the human body.

[0086] Combining Example 3 and Comparative Example 4, it can be seen that in Comparative Example 4, the raw materials of chitosan / fucoidan / beclomethasone dipropionate microcapsules were not made into microcapsules but were directly mixed. As a result, the microcapsules of beclomethasone dipropionate were released too quickly, and the cumulative release of beclomethasone dipropionate in 8 hours reached 83%. After long-term use, it is easy to cause effects on the human body. The anti-inflammatory and antibacterial oral gel prepared in Comparative Example 4 is not suitable for long-term use by the human body.

[0087] Combining Example 3 and Comparative Example 5, it can be seen that the amount of chitosan / fucoidan / beclomethasone dipropionate microcapsules in Comparative Example 5 is large, exceeding the scope of protection of this application, and the cumulative release of beclomethasone dipropionate in 8 hours reaches 68%. After long-term use, it is easy to cause effects on the human body. The anti-inflammatory and antibacterial oral gel prepared in Comparative Example 5 is not suitable for long-term use by the human body.

[0088] Combining Example 3 and Comparative Example 6, it can be seen that when the anti-inflammatory and antibacterial oral gel is prepared in Comparative Example 6, no photoinitiator is added. The anti-inflammatory and antibacterial oral gel obtained has poor adhesion performance, low cell viability, and a large cumulative release of methotrexate in 8 hours. When used in the oral cavity, it is not easy to adhere firmly, and after long-term use, it is easy to cause effects on the human body. The anti-inflammatory and antibacterial oral gel prepared in Comparative Example 5 is not suitable for long-term use by the human body.

[0089] From the instruction manual Figure 1 As can be seen from the table, over time, the anti-inflammatory and antibacterial oral gel prepared in Example 3 releases methadone propionate at a slow and uniform rate, achieving the long-term maintenance of drug efficacy required by the present application; and as can be seen from Table 1, after 8 hours, the sterilization rate of the anti-inflammatory and antibacterial oral gel is also relatively good; this proves that the anti-inflammatory and antibacterial oral gel of the present application can be successfully applied to the human oral cavity and can achieve long-term bactericidal and anti-inflammatory effects.

[0090] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. An anti-inflammatory and antibacterial oral gel, characterized in that: The invention is prepared from the following raw materials in parts by weight: 4-6 parts of chitosan / fucoidan / beclomethasone dipropionate microcapsules, 20-30 parts of sodium alginate, 20-30 parts of chitosan, 0.2-0.5 parts of a photoinitiator, and 18,000-20,000 parts of water. The chitosan / fucoidan / beclomethasone dipropionate microcapsules are prepared from chitosan, fucoidan, beclomethasone dipropionate, calcium chloride, glycerol, and water. The preparation steps of the chitosan / fucoidan / beclomethasone dipropionate microcapsules are as follows: mixing fucoidan, beclomethasone dipropionate, and glycerol to obtain a sustained-release solution; mixing chitosan, calcium chloride, and water to obtain a coating; adding the coating to the sustained-release solution and stirring to obtain the chitosan / fucoidan / beclomethasone dipropionate microcapsules; and the photoinitiator is isopropylthioxanthone.

2. The anti-inflammatory and antibacterial oral gel according to claim 1, characterized in that: The chitosan / fucoidan / beclomethasone dipropionate microcapsules are prepared from 2-4 parts of chitosan, 3-5 parts of fucoidan, 1-3 parts of beclomethasone dipropionate, 0.2-0.5 parts of calcium chloride, 5-8 parts of glycerol and 5-10 parts of water; the above raw materials are based on the chitosan / fucoidan / beclomethasone dipropionate microcapsules.

3. The anti-inflammatory and antibacterial oral gel according to claim 1, characterized in that: The coating is added to the sustained-release solution, stirred at a speed of 800 rpm-1000 rpm for 5-10 minutes, and kept at a constant temperature of 80-90° C. to form a mixed phase; the mixed phase is then pre-emulsified at a speed of 800 rpm-1000 rpm and an emulsification time of 30-40 minutes to form a mixed phase emulsion; the mixed phase emulsion is pressurized to 500-600 bar, and the cycle is repeated 3-5 times to obtain chitosan / fucoidan / beclomethasone dipropionate microcapsules.

4. A method for preparing the anti-inflammatory and antibacterial oral gel according to any one of claims 1 to 3, characterized in that: The following steps are involved: Chitosan and sodium alginate are dissolved in water, stirred thoroughly to obtain a uniform chitosan aqueous solution, dialyzed, and freeze-dried; chitosan / fucoidan / beclomethasone dipropionate microcapsules and a photoinitiator are then added and stirred uniformly to obtain a mixed solution. The mixed solution is irradiated under ultraviolet light to obtain an anti-inflammatory and antibacterial oral gel.

5. The method for preparing an anti-inflammatory and antibacterial oral gel according to claim 4, characterized in that: After the chitosan and sodium alginate are dissolved in water, the method further comprises the step of adjusting the pH to 7-9.

6. Use of the anti-inflammatory and antibacterial oral gel according to claim 1 in preparing medicines for oral gingival swelling and oral ulcers.

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