An acne composition and acne wash

Abstract

The application belongs to the field of new daily chemical materials, and discloses a kind of acne-removing composition, including sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia galanga extract, compound amino acid;The proportion of sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia galanga extract, compound amino acid is 1:0.1-1:0.01-0.2:0.01-0.1:0.1-0.5.The composition takes sodium lactate as the basis, makes full use of various extracts, and simultaneously compound compound amino acid, establishes the micro-balance of skin which has been imbalanced, so that the acne-removing effect is doubled.The application also provides a kind of cleansing milk.

Description

Technical Field

[0001] This invention relates to the field of new materials for daily chemical products, specifically to an acne-removing composition and an acne-removing facial cleanser. Background Technology

[0002] Currently, most acne treatments use either synthetic compounds or plant extracts.

[0003] With the increasing availability of extracts, plant extracts for acne treatment are becoming more and more popular in the market due to their safety and gentleness, and related products are constantly being launched.

[0004] Generally, to achieve better acne treatment results, it is recommended to use a combination of compound acne treatment products and plant extracts, such as a combination of Derma-Soothing, Piotinamide, and plant extracts. For details, see D1:CN113398028A, which discloses a toner that regulates the skin's microecological balance, including the following active ingredients: dipotassium glycyrrhizate, Piotinamide, niacinamide, menthol lactate, tea tree oil, Derma-Soothing, and frankincense extract; wherein the weight ratio of dipotassium glycyrrhizate, Piotinamide, niacinamide, menthol lactate, tea tree oil, Derma-Soothing, and frankincense extract is: 0.35-0.45 : 0.001-0.005 : 1 : 0.001-0.005 : 0.01-0.03 : 0.1-0.3 : 0.001-0.005.

[0005] A solution for pure plant extracts can be found in D2: CN115607495A, which discloses a whitening and acne-removing facial mask and its preparation method. The prepared whitening and acne-removing facial mask comprises the following raw materials in parts by weight: 2-5 parts chitosan, 2-3 parts wheat germ powder, 5-7 parts sodium alginate, 2-4 parts xanthan gum, 40-50 parts Scutellaria baicalensis fermented aqueous extract, 0.3-0.5 parts cumin-Ural licorice complex, 0.1-0.15 parts disodium EDTA, 0.05-0.1 parts hyaluronic acid, 0.1-0.2 parts sodium carboxymethyl cellulose, 0.1-0.2 parts salicylic acid, 0.1-0.2 parts vitamin C, 0.1-0.2 parts water-soluble azone, 0.2-0.3 parts preservative, 8-10 parts polyol, and 20-30 parts water.

[0006] The applicant conducted research on various plant extracts in the procurement market based on publicly available literature and found that different combinations of plant extracts have different acne-removing effects.

[0007] D3: CN108524395A discloses a set of moisturizing and whitening facial mask liquids and their preparation methods. The facial mask liquids include the following components in the indicated mass ratios: 2-6 parts glycerin, 0.1-0.5 parts SANGELOSE-60L, 0.1-0.5 parts PEG-14M, 0.05-0.2 parts sodium hyaluronate, 0.5-2 parts AA2G, 0.5-3 parts fish collagen, 0.2-0.5 parts PEG / PPG-17 / 6 copolymer, 3-8 parts 1,3-butanediol, 0.1-0.5 parts phenoxyethanol, 0.1-0.5 parts PEG-40 hydrogenated castor oil, 0.1-0.5 parts methylparaben, 0.1-0.5 parts dipotassium glycyrrhizate, 5-10 parts sodium citrate buffer, 0-1 parts plant extract, and deionized water to a total volume of 100 parts.

[0008] The product description discloses that the plant extract contains one or more of the following ingredients: grape seed extract, cucumber extract, tea tree extract, rose extract, green tea extract, milk thistle, ginseng extract, pine root extract, rhodiola rosea extract, witch hazel extract, perilla extract, apple extract, scutellaria baicalensis extract, turmeric extract, and ganoderma lucidum extract.

[0009] D4: CN115068391A discloses a whitening composition, a whitening essence containing the composition, and a whitening water. The whitening composition includes the following components: ginkgo biloba root extract; *Pseudolarix amabilis* extract; pomegranate peel extract; apple root extract; and a solvent. The whitening essence containing the whitening composition includes the following components: the whitening composition; arginine ferulic acid; 1-methylhydantoin-2-imide; allantoin; a moisturizer; a preservative; a thickener; a pH adjuster; a chelating agent; and water. The whitening water containing the whitening composition includes the following components: the whitening composition; allantoin; a moisturizer; a preservative; and water. The preparation method for all of them is as follows: mix all components evenly, filter, and age to obtain the corresponding product.

[0010] The product instructions state that the extracts of ginkgo biloba and lithospermum erythrorhizon root, along with the extract of *Hymenochloa glabra*, are beneficial in inhibiting tyrosinase activity, thus reducing melanin production. The addition of pomegranate peel extract further enhances melanin inhibition, affecting melanin transmission and achieving comprehensive whitening for a better effect. The synergistic combination of pomegranate peel extract, apple root extract, ginkgo biloba and lithospermum erythrorhizon root extract, and *Hymenochloa glabra* extract helps alleviate the damage to the skin barrier caused by these extracts during skin metabolism, accelerating barrier repair and reducing skin sensitivity. This enhances whitening effects while simultaneously promoting barrier reconstruction.

[0011] D5: CN115569080A discloses an acne-removing composition and its application, a curcumin acne-removing essence gel, and its preparation method; specifically, it relates to an acne-removing composition containing tetrahydrocurcumin and its application, as well as a curcumin acne-removing essence gel containing the tetrahydrocurcumin composition and its preparation method. The acne-removing composition is composed of tetrahydrocurcumin and potassium azeloyl diglycinate. Compared with existing technologies, it has multi-component synergistic and multi-target acne-removing and repairing effects, effectively inhibiting sebaceous gland secretion, inhibiting Propionibacterium acnes, anti-inflammatory, repairing skin damage, removing acne scars, and whitening, moisturizing, and soothing the skin.

[0012] According to its product information, tetrahydrocurcumin can enhance the antibacterial effect of components such as quaternary ammonium salt-73 that inhibit Propionibacterium acnes, thus controlling the development of acne; tetrahydrocurcumin can also enhance the wound-healing effect of asiaticoside, repairing skin damage and moisturizing and soothing the skin.

[0013] D6: CN112494408A discloses a whitening and anti-inflammatory facial mask liquid and its preparation method. The raw material weight ratio of the facial mask liquid is as follows: 5-25 parts of Trichosanthes kirilowii pulp extract, 3-6 parts of Trichosanthes kirilowii seed essential oil, 1-3 parts of grape seed oil, 5-10 parts of black garlic extract, 1-2 parts of tetrahydrocurcumin, 1-4 parts of glycerin, 30-40 parts of deionized water, 15-20 parts of sodium hyaluronate, 1-3 parts of antibacterial agent, 0.05-0.1 parts of polyacrylamide dimethyl taurate ammonium, 0.5-1 part of disodium EDTA, 1-2 parts of emulsifier, and 1-2 parts of thickener. Compared with the prior art, the facial mask liquid has no toxic side effects and has good anti-inflammatory, anti-acne, antioxidant, and whitening functions.

[0014] The product information states that tetrahydrocurcumin is a natural functional whitening ingredient with potent tyrosinase-inhibiting activity, and its whitening effect is superior to arbutin. It effectively inhibits the generation of free radicals and eliminates existing free radicals, exhibiting significant antioxidant effects. Furthermore, Trichosanthes kirilowii extract, black garlic extract, and tetrahydrocurcumin all demonstrate excellent inhibitory effects against Staphylococcus aureus, Escherichia coli, and Streptococcus viridans, especially showing significant inhibitory effects against Propionibacterium acnes.

[0015] According to existing technological records, the acne-fighting function of tetrahydrocurcumin can be confirmed.

[0016] The amount of tetrahydrocurcumin used in D5 and D6 is relatively large, reaching 0.05% in D6 and a concentration of no less than 2% in D7.

[0017] D7: CN115414308A discloses an acne-removing composition, its preparation method, and its application. The acne-removing composition includes narcissus bulb extract, purslane fermented extract, tea tree oil, and edelweiss extract. When applied to the treatment of acne and pimples, these four components primarily work in three ways: softening the stratum corneum to unclog pores, controlling oil production and clearing excess sebum, and inhibiting bacterial growth. The four components work synergistically, resulting in a composition with stronger antibacterial and antioxidant effects. Furthermore, formulations containing these four components exhibit superior acne-removing and swelling-reducing effects.

[0018] Because plant extracts are relatively expensive, we believe that combining various active ingredients can not only improve the effects of acne treatment and whitening, but also significantly reduce product costs and enhance product competitiveness.

[0019] D8: CN115368980A discloses a novel microemulsified plant-based transparent amino acid facial soap and its preparation method. The facial soap comprises an amino acid surfactant, a co-surfactant, a neutralizing agent, a microemulsion, plant herbal extracts, prebiotics, small molecule active peptides, a moisturizer, additives, and a solvent, and is prepared using a hot-melt casting method. The microemulsion is an isotropic thermodynamically stable system formed by water, oil, surfactant, and co-surfactant in appropriate proportions. Through a microemulsification process, a transparent microemulsion is prepared, dispersing moisturizing oils such as GTCC, EHP, squalane, and silk oil into the soap body to achieve a dual effect of cleansing and conditioning.

[0020] The adjuvant is one or more of the following: magnolol, sodium L-lactic acid, and dipotassium glycyrrhizate.

[0021] D9: CN115192488A discloses an acne-removing and skin-renewing essence containing betaine salicylate and its preparation method. This application utilizes a certain amount of betaine salicylate, a quaternary ammonium salt compound solution, and peony extract in combination to effectively control sebum secretion and achieve anti-inflammatory effects, fading acne scars and pigmentation. Furthermore, it is gentle, with minimal irritation, few side effects, and low damage to the skin barrier, thereby reducing the occurrence of sensitive skin.

[0022] The instruction manual states that the pH adjuster is one or more of the following: sodium lactate, citric acid, potassium hydroxide, aminomethylpropanol, sodium hydroxide, aminomethylpropanol, arginine, and triethanolamine.

[0023] It is evident that sodium lactate is used only as an adjuvant in general acne treatment products and does not participate in the functional effects of acne treatment.

[0024] D10: CN113081939A discloses a skincare composition containing stem cell extracts for acne scar removal, comprising the following ingredients in parts by weight: snail secretion filtrate 0.3-1 parts, chlorella extract 0.1-1 parts, glycerin 3-5 parts, water 20-40 parts, ethylhexylglycerin 0.3-0.8 parts, caprylyl glycol 0.35-0.6 parts, 1,2-pentanediol 1-2 parts, 1,2-hexanediol 0.35-0.6 parts, lavender flower water 5-10 parts, caviar stem cells 0.3-1.5 parts, and sunflower stem cells 0.1-1 parts.

[0025] The product manual states: Snail secretion filtrate is an external secretion of snails. As a primitive defense mechanism, it helps repair damaged areas of the body. Extracted from snail mucus, the dried snail mucus residue forms a shimmering trail. Snail mucus can effectively prevent skin aging. It contains natural collagen, high molecular weight protein, low molecular weight protein, mucopolysaccharides, etc., which strengthen connective tissue, promote elastin production, prevent premature wrinkles, effectively repair scars, promote cell migration, stimulate cell regeneration, accelerate wound healing, reduce wrinkles and scars, remove dull skin tone, lighten pigmentation, and resist free radical damage to the skin. In addition, snail mucus is also a natural antibiotic that can kill certain bacteria on the skin and eliminate acne and acne scars.

[0026] D11: CN111317703A discloses an acne-removing skincare preparation comprising the following components in parts by weight: 10-100 parts of kaempferol root extract; 0.1-1 parts of terpineol. Using kaempferol root extract and terpineol as active ingredients, an acne-removing skincare preparation was prepared. In vitro antibacterial, in vivo anti-inflammatory, and human efficacy tests confirmed its good acne-removing effect.

[0027] The product information states that a decoction of kaempferia rhizome has varying degrees of inhibitory effect on more than 10 common pathogenic bacteria in vitro. The main active ingredient of kaempferia is kaempferol, a flavonoid, which has anti-inflammatory effects and vitamin P-like activity in animals.

[0028] During the research and development process, this project discovered that by combining different ingredients, the amount of various extracts used can be significantly reduced, thereby lowering production costs.

[0029] The technical problem to be solved in this case is: how to improve the acne-removing efficacy of the composition in order to reduce product costs. Summary of the Invention

[0030] The purpose of this invention is to provide an acne-removing composition that is based on sodium lactate, makes full use of various extracts, and combines with compound amino acids to establish a micro-balance in the skin that has become unbalanced, thereby making the acne-removing effect twice as effective.

[0031] In addition, the present invention also provides a facial cleanser.

[0032] To achieve the above objectives, the present invention provides the following technical solution:

[0033] An acne-removing composition includes sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferol root extract, and complex amino acids;

[0034] The ratio of sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia root extract, and compound amino acids is 1:0.1-1:0.01-0.2:0.01-0.1:0.1-0.5.

[0035] The acne-removing composition described above also includes one or more of the following: perilla seed extract, Wenzhou mandarin orange peel extract, ginseng root extract, purslane extract, white willow bark extract, houttuynia cordata extract, forsythia fruit extract, tremella extract, peppermint extract, and atractylodes rhizome extract.

[0036] In the above-mentioned acne-removing composition, the ratio of sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia root extract, and compound amino acids is 1:0.1-0.3:0.01-0.1:0.01-0.08:0.1-0.3.

[0037] In the above-mentioned acne-removing composition, the compound amino acid is a combination of at least two of the following: serine, glycine, alanine, isoleucine, valine, proline, leucine, arginine, aspartic acid, threonine, lysine, histidine, citric acid, glutamic acid, tryptophan, tyrosine, cystine, and methionine.

[0038] The above-mentioned acne-removing composition includes sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia root extract, complex amino acids, perilla seed extract, Satsuma mandarin peel extract, ginseng root extract, and purslane extract.

[0039] The amount of each of the following extracts, including perilla seed extract, Wenzhou mandarin orange peel extract, ginseng root extract, and purslane extract, shall not exceed 20 wt% of the amount of sodium lactate.

[0040] The above-mentioned acne-removing composition includes sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia root extract, complex amino acids, perilla seed extract, Satsuma mandarin peel extract, ginseng root extract, purslane extract, white willow bark extract, houttuynia cordata extract, forsythia fruit extract, tremella extract, peppermint extract, and atractylodes rhizome extract.

[0041] The extracts of perilla seed, Wenzhou mandarin orange peel, ginseng root, purslane, white willow bark, houttuynia cordata, forsythia fruit, tremella fuciformis, peppermint, and atractylodes macrocephala rhizome each shall not exceed 10 wt% of the amount of sodium lactate.

[0042] Meanwhile, the present invention also discloses an acne-removing facial cleanser containing any of the above-mentioned acne-removing compositions, wherein the amount of the acne-removing composition does not exceed 3% of the total amount of the acne-removing facial cleanser.

[0043] In the above-mentioned acne-removing facial cleanser, the amount of the acne-removing composition accounts for 0.5-2% of the total amount of the acne-removing facial cleanser.

[0044] The aforementioned acne-removing facial cleanser also includes one or more of the following: glycerin, palmitoyl glycine, sodium cocoyl glycinate, sodium chloride, sodium methyl cocoyl taurate, glyceryl stearate SE, PEG-120 methyl glucodioleate, cocamidopropyl betaine, 1,2-hexanediol, hydroxypropyl starch, ethylhexylglycerin, polyethylene glycol-14M, butylene glycol, silica, butylated hydroxytoluene, disodium EDTA, capryloyl hydroxamic acid, and sodium benzoate, as well as the balance being water.

[0045] Compared with the prior art, the beneficial effects of the present invention are:

[0046] The present invention uses sodium lactate as the base ingredient, which is combined with snail secretion filtrate, tetrahydrocurcumin, kaempferol root extract and compound amino acids to achieve good acne removal effect within a limited dosage range.

[0047] From the perspective of the individual effects of each substance:

[0048] Sodium lactate is the sodium salt of lactic acid, produced by neutralizing the lactic acid generated during the fermentation of sugar-containing plants (such as corn and beets). Sodium lactate is a natural moisturizing factor; it constitutes one-quarter of the composition of the stratum corneum of human skin. Sodium lactate is very effective in treating skin disorders, such as extreme dryness caused by xerosis (dry skin). Lactic acid and lactate salts have antimicrobial properties and are used in anti-acne products.

[0049] Snail secretion filtrate can effectively prevent skin aging. It contains natural collagen, high molecular weight protein, low molecular weight protein, mucopolysaccharides, etc., which can strengthen connective tissue, promote elastin production, prevent wrinkles from appearing prematurely, effectively repair scars, promote cell migration, stimulate cell regeneration, accelerate wound healing, reduce wrinkles and scars, remove dull skin tone, lighten spots and resist free radical damage to the skin. In addition, snail mucus is also a natural antibiotic that can kill certain bacteria on the skin and eliminate acne and acne scars.

[0050] Tetrahydrocurcumin is a natural functional skin-whitening ingredient with potent tyrosinase-inhibiting activity, exhibiting a skin-whitening effect superior to arbutin. It effectively inhibits the generation of free radicals and eliminates existing free radicals, demonstrating significant antioxidant activity. Furthermore, Trichosanthes kirilowii extract, black garlic extract, and tetrahydrocurcumin all show excellent inhibitory effects against Staphylococcus aureus, Escherichia coli, and Streptococcus viridans, especially against Propionibacterium acnes.

[0051] Kaempferia galanga root extract has varying degrees of inhibitory effects on more than 10 common pathogenic bacteria; the main active ingredient of kaempferia galanga is kaempferol, a flavonoid, which has anti-inflammatory effects and vitamin P-like activity in animals.

[0052] Complex amino acids, different amino acids have different skin effects. When multiple amino acids are combined, they mostly have moisturizing, antibacterial and conditioning effects.

[0053] From the perspective of the synergy of various substances:

[0054] Sodium lactate, as the main component, can improve skin function disorders. Based on this, combined with the antibacterial functions of snail secretion filtrate, tetrahydrocurcumin, and kaempferia root extract, it establishes and maintains the skin's surface microecological balance. Sodium lactate can form a moisturizing film, while snail secretion filtrate and complex amino acids have both skin conditioning and moisturizing effects. Sodium lactate, snail secretion filtrate, and complex amino acids can establish a protective barrier during the skin repair process. When these five components are combined, each contributes independently to the entire process of basic skin conditioning, sterilization, establishing microecological balance, barrier establishment, and skin repair, with a more pronounced synergistic effect.

[0055] Experiments have shown that its acne-removing effect is comparable to that of Piazone and Deminshu, while also being extremely gentle on the skin. Detailed Implementation

[0056] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0057] Before describing the embodiments and comparative examples of the present invention, the specifications and origins of the raw materials used in the present invention are introduced. Reagents not listed are those commonly used in the art, as detailed in Table 1 below:

[0058] Table 1 Raw Material Information Table

[0059]

[0060]

[0061]

[0062] Examples 1-7

[0063] An acne treatment composition, the specific ingredients of which are shown in Table 2 below:

[0064] Table 2 Formulation Table (parts by weight)

[0065] Sodium lactate snail secretion filtrate Tetrahydrocurcumin Kaempferia galanga root extract Complex amino acids Example 1 1 0.5 0.1 0.05 0.35 Example 2 1 0.1 0.2 0.01 0.5 Example 3 1 1 0.01 0.1 0.1 Example 4 1 0.2 0.15 0.03 0.35 Example 5 1 0.8 0.05 0.08 0.2 Example 6 1 0.1 0.01 0.01 0.1 Example 7 1 1 0.2 0.1 0.5

[0066] Example 8

[0067] An acne-removing composition comprising sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferol root extract, complex amino acids, Curerex-AIC, and Curerex-Portulaca;

[0068] The weight ratio of sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia root extract, complex amino acids, Curerex-AIC, and Curerex-Portulaca is 1:0.5:0.1:0.05:0.35:0.2:0.15.

[0069] Example 9

[0070] An acne-removing composition comprising sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia galanga root extract, complex amino acids, Currex-AIC, Currex-Portulaca, white willow bark extract, houttuynia cordata extract, forsythia fruit extract, tremella extract, peppermint extract, and atractylodes macrocephala rhizome extract.

[0071] The weight ratios of sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia galanga root extract, complex amino acids, Currex-AIC, Currex-Portulaca, white willow bark extract, houttuynia cordata extract, forsythia fruit extract, tremella extract, peppermint extract, and atractylodes macrocephala rhizome extract are as follows:

[0072] 1:0.5:0.1:0.05:0.35:0.2:0.15:0.02:0.02:0.05:0.02:0.02:0.02.

[0073] Comparative Examples 1-6

[0074] A composition, the specific components of which are shown in Table 3 below:

[0075] Table 3 Formulation Table (parts by weight)

[0076]

[0077] Application Formulation Reference Table 4

[0078] Table 4. Cleanser Ingredients (Unit: Percentage by Weight)

[0079] Product Name Dosage Glycerin 12 Sodium cocoyl glycinate OULI-114a 24.5 Sodium cocoyl methyl taurate OULI-112 7 Ucefactant CAB 3 OULI-PG95 7.2 OULI-601 3 DOE-120 1 Sodium chloride 2.5 Hydroxypropyl starch 0.1 1,2-Hexanediol 0.8 Ethylhexylglycerin 0.1 Composition 1 water TO 100

[0080] The following facial cleanser samples 1-8 were prepared using the formulation table shown in Table 4. The preparation method can be any of the commonly used facial cleanser formulation methods in this field.

[0081] Cleanser Sample 1: The composition is as described in Example 1;

[0082] Cleanser Sample 2: The composition is Comparative Example 1;

[0083] Cleanser Sample 3: The composition is Comparative Example 2;

[0084] Cleanser Sample 4: The composition is Comparative Example 3;

[0085] Cleanser Sample 5: The composition is Comparative Example 4;

[0086] Cleanser Sample 6: The composition is Comparative Example 5;

[0087] Cleanser Sample 7: The composition is Comparative Example 6;

[0088] Cleanser Sample 8: The formula is largely the same as in Table 4, except that it does not contain any composition and contains 0.01% of Desmodium and 0.005% of Piocin.

[0089] Performance testing

[0090] 1. In vitro antibacterial test

[0091] On the culture medium inoculated with the test strain, apply 0.1 ml of quantitative sample (the examples and comparative examples in Table 5 were diluted to 1% to obtain samples); each sample was tested in triplicate, and the final result was the average of the three parallel values. The inhibition zone results of the in vitro antibacterial experiment are shown in Table 5.

[0092] Table 5 Antibacterial Results

[0093] Example 1 Example 6 Example 7 Example 8 Example 9 Staphylococcus aureus 16.7 11.4 18.3 17.1 16.0 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 5 Comparative Example 6 Staphylococcus aureus 17.5 16.9 16.8 22.4 15.6

[0094] In vitro antibacterial experiments show that:

[0095] Sodium lactate has no significant antibacterial effect; snail secretion filtrate and tetrahydrocurcumin have more obvious antibacterial effects.

[0096] 2. Evaluation of Free Radical Scavenging Effect

[0097] Test methods

[0098] (1) Processing of control materials and test samples

[0099] Sample: Dilute with pure water to a concentration of 0.1%;

[0100] Positive control (vitamin E, purity ≥96%): diluted with 95% ethanol to a positive control concentration of 0.1%;

[0101] Negative control: pure water.

[0102] (2) Experimental Operation Procedures

[0103] Set up sample tubes, sample background tubes, DPPH tubes, and solvent background tubes. Each group should have 3 parallel tubes. Add different reagent solutions to each of the four groups, shake gently, and let stand at room temperature for 5 minutes. Transfer the reaction solutions of each group into a 1 cm cuvette and measure the absorbance at 517 nm.

[0104] (3) Calculation formula

[0105] DPPH free radical scavenging rate (%) = (1 - (T - T0) / (C - CO)) * 100%

[0106] Where: T—absorbance of the sample tube, i.e., absorbance of the solution after the sample reacts with DPPH;

[0107] T0—Sample background absorbance;

[0108] The average of three absorbance values ​​of the C-DPPH tube, i.e. the absorbance of the DPPH solution without the addition of sample;

[0109] C0 — Solvent background absorbance.

[0110] (4) Data Analysis

[0111] The statistical analysis software used was SPSS. The comparison of DPPH free radical scavenging rates among the test samples, positive control, and negative control was performed using independent samples test.

[0112] (5) Results Analysis

[0113] Results are shown in Table 6.

[0114] Table 6. DPPH free radical scavenging rate (%)

[0115]

[0116] 3. Evaluation of acne treatment effects on volunteers

[0117] This experiment referenced the evaluation method of T / TDCA 004—2021 "Test Method for Acne-Removing Efficacy of Cosmetics" to conduct a human efficacy evaluation experiment on the anti-wrinkle effect of the samples. A total of 80 subjects participated in this experiment, with 0 withdrawing and 80 valid subjects.

[0118] After 80 subjects used the sample continuously for 14 days, the difference between the skin a* value of the sample area and the baseline value (14 days ago) was used as an evaluation index for acne treatment effect.

[0119] 1. Subject Information

[0120] Gender of participants: 50 women and 30 men.

[0121] Subjects' ages: 25–60 years.

[0122] Subject skin requirements: The subject's facial skin must have a certain number of pimples and acne, but not nodules or cysts that are difficult to improve with clinical preparations.

[0123] This test included 80 healthy participants aged 25 to 60 years, and the final valid data was 80 participants.

[0124] 2. Sample Usage Instructions

[0125] Application area: Face.

[0126] Frequency of use: Use in the morning and evening.

[0127] Usage period: 2 weeks.

[0128] How to use: Apply by dotting, selecting the acne or pimple area as the sample area. Apply twice a day;

[0129] Sample information: Facial cleanser samples 1-8;

[0130] 3. Testing Methods

[0131] The skin color probe was used to test the skin redness value;

[0132] Before the test: When the subjects arrive, they are visually assessed by trained professional testers, and those who meet the inclusion criteria are enrolled in the group;

[0133] Cleanse face and wait: Subjects cleanse their face with facial cleanser and dry their skin with a dry facial tissue. They then sit quietly for 20 minutes in a laboratory at a temperature of 21±1℃ and 50±5%RH.

[0134] Baseline skin data collection for test subjects: Laboratory technicians used a skin color probe (Colorimeter CL400) and a facial image analyzer (VISIA7) to measure relevant baseline indicators of the test sites on the subjects;

[0135] Laboratory technicians guided subjects on how to use the product according to the product usage requirements and application sites, and provided written test precautions and product usage instructions;

[0136] The subjects received their samples and left the laboratory.

[0137] After the test: Subjects were followed up, cleaned their faces and waited: their faces were cleaned with facial cleanser and their skin was dried with a dry facial tissue. They sat quietly for 20 minutes in a laboratory at a temperature of 21±1℃ and 50±5%RH.

[0138] D28 data collection of test subjects' skin: Laboratory technicians used a skin color probe (Colorimeter CL400) and a facial image analyzer (VISIA7) to measure relevant indicators of the test sites of the subjects;

[0139] Staff checked the users' usage records and weighed the product;

[0140] The testing process has ended.

[0141] 4. Test Results

[0142] Before the test, the mean a* value of the skin of the 8 groups of subjects was within the range of 12.7±0.3, indicating that the skin condition of the 8 groups of subjects was evenly distributed and the results of the 8 groups of subjects were of reference value.

[0143] Calculation formula

[0144] Skin a* value decrease rate % = (YX)*100% / Y;

[0145] X represents the average skin a* value of each group of subjects after 14 days;

[0146] Y represents the average skin a* value of each group of subjects 14 days prior.

[0147] The test results are shown in Table 7 below:

[0148] Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Experimental results 14.55 8.35 6.27 8.04 9.29 10.93 7.31 13.26

[0149] Results analysis:

[0150] 1. As shown in Table 5, sodium lactate has no significant antibacterial effect; snail secretion filtrate and tetrahydrocurcumin have relatively obvious antibacterial effects; as shown in Table 6, amino acids and sodium lactate do not contribute much to the free radical scavenging effect; as shown in Tables 5-7, antibacterial properties and free radical scavenging effects can be used as evaluation indicators for acne treatment, but in this invention, they are not the only reference indicators for determining the acne treatment effect, and there is a certain difference between the acne treatment effect and the antibacterial and free radical scavenging effects.

[0151] 2. As can be seen from samples 1 and 6 in Table 7, both sodium lactate and sodium hyaluronate are effective ingredients for moisturizing and film formation. However, since sodium hyaluronate is not as effective as sodium lactate in regulating skin dysfunction, sterilization, and stratum corneum repair, there is a significant difference in their acne-removing effects.

[0152] 3. As can be seen from Sample 1 and Samples 2-5 in Table 7, tetrahydrocurcumin has a decisive influence on the acne-removing function, while the influence of compound amino acids and kaempferia root extract is basically equal; snail secretion filtrate and tetrahydrocurcumin have similar influence on the acne-removing function.

[0153] 4. As can be seen from Sample 1 and Sample 6 in Table 7, the actual dosage of snail secretion filtrate, tetrahydrocurcumin, kaempferia root extract and compound amino acids in Sample 6 is significantly increased. However, there is still a 24% difference compared to Sample 1. This also indicates that sodium lactate plays a basic role in skin function, moisturizing and film formation in this formula, and is the basis for the functional expression of other active ingredients.

[0154] 5. As can be seen from Sample 1 and Sample 8 in Table 7, this solution has a relatively obvious effect on acne treatment.

[0155] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

Claims

1. An acne-removing composition, characterized in that, Including sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia root extract, complex amino acids, perilla seed extract, Satsuma mandarin peel extract, ginseng root extract, and purslane extract; The total amount of the perilla seed extract, Wenzhou mandarin orange peel extract, and ginseng root extract shall not exceed 20 wt% of the amount of sodium lactate. The purslane extract shall not exceed 15 wt% of the amount of sodium lactate used; The weight ratio of sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia root extract, and complex amino acids is 1:

1. 0.5：0.1：0.05:0.35； The complex amino acid is a combination of serine, glycine, alanine, isoleucine, valine, leucine, proline, arginine, threonine, aspartic acid, lysine, glutamic acid, histidine, methionine, tryptophan, cystine, and tyrosine.

2. The acne-removing composition according to claim 1, characterized in that, Including sodium lactate, snail secretion filtrate, tetrahydrocurcumin, kaempferia root extract, complex amino acids, perilla seed extract, Satsuma mandarin peel extract, ginseng root extract, purslane extract, white willow bark extract, houttuynia cordata extract, forsythia fruit extract, tremella extract, peppermint extract, and atractylodes rhizome extract. The total amount of the perilla seed extract, Wenzhou mandarin orange peel extract, and ginseng root extract shall not exceed 20 wt% of the amount of sodium lactate. The purslane extract shall not exceed 15 wt% of the amount of sodium lactate used; The amounts of each of the following extracts—white willow bark extract, houttuynia cordata extract, tremella extract, peppermint extract, and atractylodes macrocephala rhizome extract—were 2 wt% of the amount of sodium lactate used. The amount of Forsythia fruit extract used is 5 wt% of the amount of sodium lactate used; The complex amino acid is a combination of serine, glycine, alanine, isoleucine, valine, leucine, proline, arginine, threonine, aspartic acid, lysine, glutamic acid, histidine, methionine, tryptophan, cystine, and tyrosine.

3. An acne-removing facial cleanser, characterized in that, The product contains the acne-removing composition as described in claim 1 or 2, wherein the amount of the acne-removing composition does not exceed 3 wt% of the total amount of the acne-removing facial cleanser.

4. The acne-removing facial cleanser according to claim 3, characterized in that, The amount of the acne-removing composition is 0.5-2 wt% of the total amount of the acne-removing facial cleanser.

5. The acne-removing facial cleanser according to claim 3, characterized in that, It also includes one or more of the following: glycerol, palmitoyl glycine, sodium cocoyl glycinate, sodium chloride, sodium methyl cocoyl taurate, glyceryl stearate SE, PEG-120 methyl glucoside dioleate, cocamidopropyl betaine, 1,2-hexanediol, hydroxypropyl starch, ethylhexylglycerin, polyethylene glycol-14M, butanediol, silica, butylated hydroxytoluene, disodium EDTA, capryloyl hydroxamic acid, and sodium benzoate, and the balance being water.

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