A vaginal cleansing composition and method of vaginal cleansing

Abstract

The present invention relates to a vaginal cleaning composition comprising a bacteriostatic complex and a lactic acid bacteria ferment obtained by co-fermentation of a plurality of lactic acid bacteria strains in appropriate proportions, wherein the bacteriostatic complex can effectively inhibit vaginal inflammation pathogens, and the lactic acid bacteria ferment can promote the growth of probiotics in the vagina. Therefore, the above-mentioned vaginal cleaning composition can effectively inhibit vaginal inflammation pathogens, while avoiding the change of the acidic environment of the vagina caused by the inhibition of probiotics in the vagina by the bacteriostatic complex. A cleaning method for the vagina using the above-mentioned vaginal cleaning composition is also disclosed.

Description

Technical Field

[0001] This invention relates to the field of personal hygiene products, specifically to a vaginal cleansing composition and a vaginal cleansing method. Background Technology

[0002] Studies indicate that over 50% of modern women experience urinary tract or vaginal infections. The most common cause is vulvovaginal infection, which includes candidiasis, bacterial vaginosis, and trichomoniasis. Candidiasis and bacterial vaginosis are caused by microorganisms. Because the vagina is connected to the outside world and is close to the anus, which harbors many potential pathogens, it is easily invaded by pathogens. Therefore, inhibiting vaginitis pathogens is an important issue.

[0003] Products that inhibit vaginal pathogens have been developed; however, the antibacterial ingredients in these products not only inhibit vaginal pathogens but also suppress beneficial bacteria in the vagina. Acid-loving beneficial bacteria continuously produce acid to maintain a healthy acidic environment in the vagina. However, suppressing these beneficial bacteria may disrupt the vaginal acidic environment, causing an increase in vaginal pH, which could lead to the proliferation of pathogens and increase the risk of infection.

[0004] Therefore, developing products that can inhibit vaginal pathogens and promote the growth of probiotics is the current goal. Summary of the Invention

[0005] This invention provides a vaginal cleansing composition and a vaginal cleansing method to solve the technical problem that in the prior art, vaginal cleansing compositions inhibit probiotics, leading to changes in the vaginal environment and increasing the risk of infection.

[0006] To solve the above-mentioned technical problems, the technical solution provided by the present invention is as follows:

[0007] This invention relates to a vaginal cleansing composition comprising:

[0008] Lactic acid bacteria fermentation product, using a culture medium containing at least one of milk, milk powder, whey, casein, soy protein, and collagen, and containing isolated Streptococcus thermophilus (Streptococcus thermophilus). 嗜热链球菌 ), Lactobacillus salivarius ( 唾液乳杆菌 Lactobacillus rhamnosus ( 鼠李糖乳杆菌 ) and Bifidobacterium bifidum ( 两歧双歧杆菌 It is obtained by co-fermentation of Streptococcus thermophilus, Lactobacillus salivarius, Lactobacillus rhamnosus, and Bifidobacterium bifidum; the ratio of the amounts of Streptococcus thermophilus, Lactobacillus salivarius, Lactobacillus rhamnosus, and Bifidobacterium bifidum is (0.8-1.2):(1.4-1.7):(1.3-1.7):(0.8-1.2).

[0009] The antibacterial complex comprises wild soybean seed extract, houttuynia cordata extract, scutellaria baicalensis root extract, neem leaf extract, rehmannia glutinosa root extract, white willow bark extract, phellodendron bark extract, o-cymene-5-ol, lactobacillus fermentation filtrate of pear juice, glyceryl caprylate, ethylhexylglycerin, PEG-60 hydrogenated castor oil, glycolic acid, butylene glycol, and water;

[0010] Excipients, diluents, or carriers.

[0011] The content of lactic acid fermentation product is 0.4-10%, and preferably, the content of antibacterial complex is 5-15%.

[0012] The antibacterial complex in this invention is used to inhibit the growth of microorganisms, including vaginal pathogens such as Candida albicans, Staphylococcus aureus, and Escherichia coli. However, it also inhibits beneficial bacteria in the vagina, such as Lactobacillus and Bifidobacterium. The lactic acid bacteria ferment in this invention promotes the growth of beneficial bacteria such as Lactobacillus and Bifidobacterium. Therefore, the combination of the antibacterial complex and the lactic acid bacteria ferment ensures that the beneficial bacteria are not completely inhibited by the antibacterial complex, thus providing a certain degree of growth conditions and achieving the effect of inhibiting vaginal pathogens and promoting the growth of beneficial bacteria.

[0013] Preferably, the lactic acid bacteria ferment broth is a fermentation broth containing inactivated bacterial cells or with bacterial cells removed, or a dried powder of the fermentation broth; Streptococcus thermophilus ( 嗜热链球菌 ), Lactobacillus salivarius ( 唾液乳杆菌 Lactobacillus rhamnosus ( 鼠李糖乳杆菌 ) and Bifidobacterium bifidum ( 两歧双歧杆菌 The ratio of the amounts of ) is 2:3:3:2.

[0014] Preferably, the antibacterial complex comprises 0.006%~0.008% of wild soybean seed extract, 0.014%~0.019% of houttuynia cordata extract, 0.016%~0.02% of scutellaria baicalensis root extract, 0.018%~0.022% of neem leaf extract, 0.038%~0.042% of rehmannia glutinosa root extract, 0.046%~0.052% of willow bark extract, and 0.123%~0. 0.127% of Phellodendron bark extract, 0.23%–0.27% of O-cymene-5-ol, 0.68%–0.72% of Lactobacillus fermentation filtrate of pear juice, 0.8%–1.2% of glyceryl caprylate, 0.8%–1.2% of ethylhexylglycerin, 3.6%–4.2% of PEG-60 hydrogenated castor oil, 5.3%–5.9% of glycolic acid, 38.3%–42.1% of butylene glycol, and water.

[0015] Preferably, it also includes a thickener, which is a combination of natural glucan gum, carbomer, or the above, and the thickener content is 0.2-5%.

[0016] Preferably, it further includes a first conditioning agent, which is a combination of sodium hyaluronate, an anti-allergy complex, allantoin, or the above, and the content of the first conditioning agent is 0.005-5%, wherein the anti-allergy complex contains deep sea water and zucchini seed extract.

[0017] Preferably, it further includes a second conditioning agent, which comprises a first complex, a second complex, menthol oil, tea tree oil, aloe vera extract, galangal extract, nano silver, or a combination thereof, in an amount of 0.1-8%, wherein the first complex comprises dipropylene glycol, hydroxyacetophenone, octyl ethylene glycol, and dipotassium glycyrrhizate, and the second complex comprises pepper fruit extract, Pulsatilla root extract, and moss extract.

[0018] The thickener, first conditioning agent, and second conditioning agent are all conventional thickeners and conditioning agents in the art. The specific amounts of each component can be set by those skilled in the art as needed, and will not be elaborated here. Preferably, the excipient, diluent, or carrier comprises sodium hydroxide, triethanolamine, glycerol, or a combination thereof, and water. The content of sodium hydroxide, triethanolamine, glycerol, or a combination thereof is 0.05-10%, with the remainder made up to 100% with water. The excipient, diluent, or carrier is conventional technology in the art, and the specific amounts of each component can be set by those skilled in the art as needed, and will not be elaborated here.

[0019] Preferably, it is a tablet, capsule, powder, cream, spray, gel, solution, powder, lotion, or lotion.

[0020] A method for cleaning the vagina using the vaginal cleaning composition of the present invention.

[0021] Compared with the prior art, the technical solution of this invention has the following advantages:

[0022] 1. This invention provides a vaginal cleansing composition comprising lactic acid bacteria ferment, which simultaneously inhibits pathogens and promotes the growth of beneficial bacteria. Furthermore, the ferment obtained by fermenting Streptococcus thermophilus, Lactobacillus salivarius, Lactobacillus rhamnosus, and Bifidobacterium bifidum in a ratio of 2:3:3:2 has the best effect of inhibiting pathogens and promoting the growth of beneficial bacteria, thus enabling this invention to simultaneously inhibit vaginitis pathogens and promote the growth of beneficial bacteria.

[0023] 2. This invention includes an antibacterial complex, which, when combined with lactic acid bacteria ferment, can strongly inhibit the growth of vaginal pathogens. Furthermore, the lactic acid bacteria ferment does not significantly affect probiotics, thus greatly enhancing the effectiveness of this invention in inhibiting vaginal pathogens. Attached Figure Description

[0024] Figure 1 The results show that lactic acid bacteria fermentation products inhibit vaginal pathogens and probiotics.

[0025] Figure 2 These are the results of the detection of the survival rate of different antibacterial components against vaginal pathogens and probiotics.

[0026] In the figure, the symbol * indicates p>0.1, and the symbol ** indicates p>0.05, which means there is a statistically significant difference. Detailed Implementation

[0027] To further understand the content of this invention, the invention will be described in detail with reference to the embodiments. The following embodiments are used to illustrate the invention, but are not intended to limit the scope of the invention. Example 1

[0028] The vaginal cleansing composition in this embodiment includes the following components:

[0029] Lactic acid bacteria fermentation product, using a culture medium containing at least one of milk, milk powder, whey, casein, soy protein, and collagen, and containing isolated Streptococcus thermophilus (Streptococcus thermophilus). 嗜热链球菌 ), Lactobacillus salivarius ( 唾液乳杆菌 Lactobacillus rhamnosus ( 鼠李糖乳杆菌 ) and Bifidobacterium bifidum ( 两歧双歧杆菌 It is obtained through co-fermentation;

[0030] The antibacterial complex contains 0.006%–0.008% of wild soybean seed extract, 0.014%–0.019% of houttuynia cordata extract, 0.016%–0.02% of scutellaria baicalensis root extract, 0.018%–0.022% of neem leaf extract, 0.038%–0.042% of rehmannia glutinosa root extract, 0.046%–0.052% of willow bark extract, 0.123%–0.127% of phellodendron bark extract, 0.23%–0.27% of o-cymene-5-ol, 0.68%–0.72% of Lactobacillus fermentation filtrate from pear juice, 0.8%–1.2% of glyceryl caprylate, 0.8%–1.2% of ethylhexylglycerin, and 3.6%–4.2% of PEG-60. Hydrogenated castor oil, 5.3%–5.9% glycolic acid, 38.3%–42.1% butanediol, and water;

[0031] Excipients, diluents, or carriers.

[0032] The antibacterial complex in this invention is used to inhibit the growth of microorganisms, including vaginal pathogens such as Candida albicans, Staphylococcus aureus, and Escherichia coli. However, it also inhibits beneficial bacteria in the vagina, such as Lactobacillus and Bifidobacterium. The lactic acid bacteria ferment in this invention promotes the growth of beneficial bacteria such as Lactobacillus and Bifidobacterium. Therefore, the combination of the antibacterial complex and the lactic acid bacteria ferment ensures that the beneficial bacteria are not completely inhibited by the antibacterial complex, thus providing a certain degree of growth conditions and achieving the effect of inhibiting vaginal pathogens and promoting the growth of beneficial bacteria.

[0033] It should be noted that *Streptococcus thermophilus* is used as an auxiliary fermentation agent; *Lactobacillus salivarius*, *Lactobacillus rhamnosus*, and *Bifidobacterium bifidum* can be selected from strains known to inhibit the activity of vaginal pathogens, therefore the lactic acid bacteria fermentation product of the present invention is not limited to fermentation with specific strains of the above-mentioned species. The lactic acid bacteria fermentation product can be sterilized by heat to obtain a fermentation broth containing inactive bacterial cells, or obtained by centrifugation to remove bacterial cells, or the lactic acid bacteria fermentation product can be further dried into a dry powder of the fermentation broth.

[0034] Regarding the determination of the dosage ratio of each bacterial strain in the lactic acid bacteria fermentation product in this embodiment:

[0035] 1) A culture medium containing milk, milk powder, whey, casein, soy protein, collagen or a combination thereof as a base was used. The isolated Streptococcus thermophilus, Lactobacillus salivarius, Lactobacillus rhamnosus and Bifidobacterium bifidum were fermented in three ratios of 1:1:1:1, 2:1:1:2 and 2:3:3:2. Liquid culture medium containing 5% of the three groups of lactic acid bacteria fermentation products prepared above was quantified into 4.9 ml culture tubes as experimental groups. Liquid culture medium without added lactic acid bacteria fermentation products was added to culture tubes as control groups. Pure water was added to culture tubes as control groups.

[0036] 2) Add 0.1 ml of activated vaginal pathogens (Candida albicans, Staphylococcus aureus, Escherichia coli) and probiotics (Lactobacillus, Bifidobacterium) to different culture tubes to achieve a bacterial concentration of approximately 10. 3 CFU / ml, cultured according to the corresponding culture conditions, and diluted with an appropriate sequence after 6 hours of culture;

[0037] 3) Take 1 ml of bacterial culture from each of the experimental, control, and control groups, and mix it with 15 ml of the corresponding culture medium for vaginal pathogens and probiotics, respectively. Incubate in an incubator under suitable conditions for 24 to 48 hours. After the corresponding incubation time, remove the culture trays and count the colonies of pathogens and probiotics to estimate the original bacterial count. Using the bacterial count of the control group as a baseline, calculate the survival rate of the control and experimental groups. The survival rate is calculated as follows:

[0038]

[0039] The results of the detection of lactic acid bacteria fermentation products in inhibiting vaginal pathogens and probiotics are shown in Table 1 and... Figure 1 As shown in Table 1, the results indicate that lactic acid bacteria fermentation products obtained from co-fermentation of different proportions of lactic acid bacteria strains exhibit varying antibacterial effects against vaginitis pathogens and probiotics. Among them, the lactic acid bacteria fermentation product obtained from co-fermentation of *Streptococcus thermophilus*, *Lactobacillus salivarius*, *Lactobacillus rhamnosus*, and *Bifidobacterium bifidum* in a 2:3:3:2 ratio showed the best antibacterial rate against vaginitis pathogens, meaning the survival rate of vaginitis pathogens was the lowest. It should be noted that the lactic acid bacteria fermentation product in the 2:3:3:2 experimental group showed the best growth-promoting effect on probiotics (lactobacters and bifidobacteria), meaning the survival rate of probiotics was greater than 200%. The above test results show that lactic acid bacteria fermentation products obtained from co-fermentation of multiple lactic acid bacteria strains in specific proportions have unexpected effects on inhibiting vaginitis pathogens and promoting the growth of probiotics.

[0040] Table 1: Survival rates of vaginal pathogens and probiotics in lactic acid bacteria fermentation products obtained from co-fermentation of multiple lactic acid bacteria strains in different proportions.

[0041]

[0042] Therefore, in this embodiment, Streptococcus thermophilus ( 嗜热链球菌 ), Lactobacillus salivarius ( 唾液乳杆菌 Lactobacillus rhamnosus ( 鼠李糖乳杆菌 ) and Bifidobacterium bifidum ( 两歧双歧杆菌 The ratio of the amounts of ) is 2:3:3:2.

[0043] In this embodiment, the content of lactic acid bacteria ferment is 5%, and the content of antibacterial complex is 10%.

[0044] This embodiment also includes a thickener, which is a combination of natural polyglucan, carbomer, or more, with a thickener content of 0.2-5%. This embodiment also includes a first conditioning agent, which is a combination of sodium hyaluronate, an anti-allergy complex, allantoin, or more, with a first conditioning agent content of 0.005-5%, wherein the anti-allergy complex comprises deep-sea water and zucchini seed extract. The first conditioning agent has anti-inflammatory and soothing effects. This embodiment also includes a second conditioning agent, which comprises a first complex, a second complex, menthol oil, tea tree oil, aloe vera extract, galangal extract, nano-silver, or more, with a content of 0.1-8%, wherein the first complex comprises dipropylene glycol, hydroxyacetophenone, octyl ethylene glycol, and dipotassium glycyrrhizate, and the second complex comprises pepper fruit extract, pulsatilla root extract, and moss extract. The second conditioning agent has antiseptic, anti-inflammatory, and soothing effects. In this embodiment, the excipient, diluent, or carrier comprises sodium hydroxide, triethanolamine, glycerol, or a combination thereof, and water. The content of sodium hydroxide, triethanolamine, glycerol, or a combination thereof is 0.05-10%, and the remainder is made up to 100% with water. The thickener, first conditioning agent, second conditioning agent, excipient, diluent, or carrier mentioned above are all conventional technologies in the art. The specific amounts of each component can be set by those skilled in the art according to their needs, and will not be elaborated here.

[0045] The composition in this embodiment is in the form of tablets, but it can also be in the form of capsules, powders, creams, sprays, gels, solutions, powders, creams, or lotions. Example 2

[0046] The vaginal cleansing composition in this embodiment includes the following components:

[0047] Lactic acid bacteria fermentation product, using a culture medium containing at least one of milk, milk powder, whey, casein, soy protein, and collagen, and containing isolated Streptococcus thermophilus (Streptococcus thermophilus). 嗜热链球菌 ), Lactobacillus salivarius ( 唾液乳杆菌 Lactobacillus rhamnosus ( 鼠李糖乳杆菌) and Bifidobacterium bifidum ( 两歧双歧杆菌 It is obtained through co-fermentation;

[0048] The antibacterial complex contains 0.006%–0.008% of wild soybean seed extract, 0.014%–0.019% of houttuynia cordata extract, 0.016%–0.02% of scutellaria baicalensis root extract, 0.018%–0.022% of neem leaf extract, 0.038%–0.042% of rehmannia glutinosa root extract, 0.046%–0.052% of willow bark extract, 0.123%–0.127% of phellodendron bark extract, 0.23%–0.27% of o-cymene-5-ol, 0.68%–0.72% of Lactobacillus fermentation filtrate from pear juice, 0.8%–1.2% of glyceryl caprylate, 0.8%–1.2% of ethylhexylglycerin, and 3.6%–4.2% of PEG-60. Hydrogenated castor oil, 5.3%–5.9% glycolic acid, 38.3%–42.1% butanediol, and water;

[0049] Excipients, diluents, or carriers.

[0050] The antibacterial complex in this invention is used to inhibit the growth of microorganisms, including vaginal pathogens such as Candida albicans, Staphylococcus aureus, and Escherichia coli. However, it also inhibits beneficial bacteria in the vagina, such as Lactobacillus and Bifidobacterium. The lactic acid bacteria ferment in this invention promotes the growth of beneficial bacteria such as Lactobacillus and Bifidobacterium. Therefore, the combination of the antibacterial complex and the lactic acid bacteria ferment ensures that the beneficial bacteria are not completely inhibited by the antibacterial complex, thus providing a certain degree of growth conditions and achieving the effect of inhibiting vaginal pathogens and promoting the growth of beneficial bacteria.

[0051] It should be noted that *Streptococcus thermophilus* is used as an auxiliary fermentation agent; *Lactobacillus salivarius*, *Lactobacillus rhamnosus*, and *Bifidobacterium bifidum* can be selected from strains known to inhibit the activity of vaginal pathogens, therefore the lactic acid bacteria fermentation product of the present invention is not limited to fermentation with specific strains of the above-mentioned species. The lactic acid bacteria fermentation product can be sterilized by heat to obtain a fermentation broth containing inactive cells or obtained by centrifugation to remove the cells, or the lactic acid bacteria fermentation product can be further dried into a dry powder of the fermentation broth.

[0052] In this embodiment, Streptococcus thermophilus ( 嗜热链球菌 ), Lactobacillus salivarius ( 唾液乳杆菌 Lactobacillus rhamnosus ( 鼠李糖乳杆菌 ) and Bifidobacterium bifidum ( 两歧双歧杆菌 The ratio of the amounts of ) is 2:3:3:2.

[0053] In this embodiment, the content of lactic acid bacteria ferment is 1%, and the content of antibacterial complex is 14%.

[0054] This embodiment also includes a thickener, which is a combination of natural polyglucan, carbomer, or more, with a thickener content of 0.2-5%. This embodiment also includes a first conditioning agent, which is a combination of sodium hyaluronate, an anti-allergy complex, allantoin, or more, with a conditioning agent content of 0.005-5%, wherein the anti-allergy complex contains deep-sea water and zucchini seed extract. It has anti-inflammatory and soothing effects. This embodiment also includes a second conditioning agent, which includes the first complex, the second complex, menthol oil, tea tree oil, aloe vera extract, galangal extract, nano-silver, or more, with a content of 0.1-8%, wherein the first complex contains dipropylene glycol, hydroxyacetophenone, octyl ethylene glycol, and dipotassium glycyrrhizate, and the second complex contains zanthoxylum bungeanum fruit extract, Pulsatilla chinensis root extract, and moss extract. It has antiseptic, anti-inflammatory, and soothing effects. In this embodiment, the excipient, diluent, or carrier comprises a combination of sodium hydroxide, triethanolamine, and glycerol, and water. The content of the combination of sodium hydroxide, triethanolamine, and glycerol is 0.05-10%, and the remainder is made up to 100% with water. The thickener, first conditioning agent, second conditioning agent, excipient, diluent, or carrier mentioned above are all conventional technologies in the art. The specific amounts of each component can be set by those skilled in the art according to their needs, and will not be elaborated here.

[0055] The composition in this embodiment is in the form of tablets, but it can also be in the form of capsules, powders, creams, sprays, gels, solutions, powders, creams, or lotions. Example 3

[0056] The vaginal cleansing composition in this embodiment includes the following components:

[0057] Lactic acid bacteria fermentation product, using a culture medium containing at least one of milk, milk powder, whey, casein, soy protein, and collagen, and containing isolated Streptococcus thermophilus (Streptococcus thermophilus). 嗜热链球菌 ), Lactobacillus salivarius ( 唾液乳杆菌 Lactobacillus rhamnosus ( 鼠李糖乳杆菌 ) and Bifidobacterium bifidum ( 两歧双歧杆菌 It is obtained through co-fermentation;

[0058] The antibacterial complex contains 0.006%–0.008% of wild soybean seed extract, 0.014%–0.019% of houttuynia cordata extract, 0.016%–0.02% of scutellaria baicalensis root extract, 0.018%–0.022% of neem leaf extract, 0.038%–0.042% of rehmannia glutinosa root extract, 0.046%–0.052% of willow bark extract, 0.123%–0.127% of phellodendron bark extract, 0.23%–0.27% of o-cymene-5-ol, 0.68%–0.72% of Lactobacillus fermentation filtrate from pear juice, 0.8%–1.2% of glyceryl caprylate, 0.8%–1.2% of ethylhexylglycerin, and 3.6%–4.2% of PEG-60. Hydrogenated castor oil, 5.3%–5.9% glycolic acid, 38.3%–42.1% butanediol, and water;

[0059] Excipients, diluents, or carriers.

[0060] The antibacterial complex in this invention is used to inhibit the growth of microorganisms, including vaginal pathogens such as Candida albicans, Staphylococcus aureus, and Escherichia coli. However, it also inhibits beneficial bacteria in the vagina, such as Lactobacillus and Bifidobacterium. The lactic acid bacteria ferment in this invention promotes the growth of beneficial bacteria such as Lactobacillus and Bifidobacterium. Therefore, the combination of the antibacterial complex and the lactic acid bacteria ferment ensures that the beneficial bacteria are not completely inhibited by the antibacterial complex, thus providing a certain degree of growth conditions and achieving the effect of inhibiting vaginal pathogens and promoting the growth of beneficial bacteria.

[0061] It should be noted that *Streptococcus thermophilus* is used as an auxiliary fermentation agent; *Lactobacillus salivarius*, *Lactobacillus rhamnosus*, and *Bifidobacterium bifidum* can be selected from strains known to inhibit the activity of vaginal pathogens, therefore the lactic acid bacteria fermentation product of the present invention is not limited to fermentation with specific strains of the above-mentioned species. The lactic acid bacteria fermentation product can be sterilized by heat to obtain a fermentation broth containing inactive cells or obtained by centrifugation to remove the cells, or the lactic acid bacteria fermentation product can be further dried into a dry powder of the fermentation broth.

[0062] In this embodiment, Streptococcus thermophilus ( 嗜热链球菌 ), Lactobacillus salivarius ( 唾液乳杆菌 Lactobacillus rhamnosus ( 鼠李糖乳杆菌 ) and Bifidobacterium bifidum ( 两歧双歧杆菌 The ratio of the amounts of ) is 2:3:3:2.

[0063] In this embodiment, the content of lactic acid bacteria fermentation product is 9.8%, and the content of antibacterial complex is 6%.

[0064] This embodiment also includes a thickener, which is a combination of natural polyglucan, carbomer, or more, with a thickener content of 0.2-5%. This embodiment also includes a first conditioning agent, which is a combination of sodium hyaluronate, an anti-allergy complex, allantoin, or more, with a conditioning agent content of 0.005-5%, wherein the anti-allergy complex contains deep-sea water and zucchini seed extract. It has anti-inflammatory and soothing effects. This embodiment also includes a second conditioning agent, which includes the first complex, the second complex, menthol oil, tea tree oil, aloe vera extract, galangal extract, nano-silver, or more, with a content of 0.1-8%, wherein the first complex contains dipropylene glycol, hydroxyacetophenone, octyl ethylene glycol, and dipotassium glycyrrhizate, and the second complex contains zanthoxylum bungeanum fruit extract, Pulsatilla chinensis root extract, and moss extract. It has antiseptic, anti-inflammatory, and soothing effects. In this embodiment, the excipient, diluent, or carrier comprises a combination of sodium hydroxide, triethanolamine, and glycerol, and water. The content of the combination of sodium hydroxide, triethanolamine, and glycerol is 0.05-10%, and the remainder is made up to 100% with water. The thickener, first conditioning agent, second conditioning agent, excipient, diluent, or carrier mentioned above are all conventional technologies in the art. The specific amounts of each component can be set by those skilled in the art according to their needs, and will not be elaborated here.

[0065] The composition in this embodiment is in the form of tablets, but it can also be in the form of capsules, powders, creams, sprays, gels, solutions, powders, creams, or lotions.

[0066] Comparative test of the efficacy of the vaginal cleansing composition in this invention:

[0067] 1) Examples 1-3 were used as the experimental group. Liquid culture medium containing the composition of Examples 1-3 was quantified into 4.9 ml culture tubes. Liquid culture medium that did not contain the antibacterial complex and lactic acid fermentation product but had the same composition as Example 1 was quantified into 4.9 ml culture tubes as the control group.

[0068] 2) Add 0.1 ml of activated vaginal pathogens (Candida albicans, Staphylococcus aureus, Escherichia coli) and probiotics (Lactobacillus, Bifidobacterium) to different culture tubes to achieve a bacterial concentration of approximately 10. 3 CFU / ml, cultured according to the corresponding culture conditions, and diluted with an appropriate sequence after 6 hours of culture;

[0069] 3) Take 1 ml of bacterial culture from each of the experimental and control groups, mix it with 15 ml of the corresponding culture medium for vaginal pathogens and probiotics, respectively, and incubate them upside down in an incubator under suitable conditions for 24 to 48 hours. After the corresponding incubation time is reached, remove the culture trays and count the colonies of pathogens and probiotics to estimate the original bacterial count. Use the bacterial count of the control group as a baseline to calculate the survival rate of the experimental group. The survival rate is calculated as follows:

[0070]

[0071] The lactic acid bacteria ferment prepared in a ratio of 2:3:3:2 showed good growth-promoting effects on probiotics, but its inhibitory effect on vaginal pathogens needs to be strengthened (see reference). Figure 1 (and Table 1). And from Figure 2 As shown in Table 2, the antibacterial composition of the present invention includes an antibacterial complex and a lactic acid bacteria ferment obtained by co-fermentation of Streptococcus thermophilus, Lactobacillus salivarius, Lactobacillus rhamnosus and Bifidobacterium bifidum in a ratio of 2:3:3:2. It has a good antibacterial effect on vaginal pathogens and also promotes the growth of probiotics.

[0072] The test results above show that the vaginal cleansing composition of the present invention contains an antibacterial complex and lactic acid bacteria ferment obtained by co-fermentation of multiple lactic acid bacteria strains in a specific ratio. It not only has a good antibacterial effect against vaginal pathogens but also promotes the growth of beneficial bacteria in the vagina, thus maintaining a healthy acidic environment in the user's vagina. It should also be noted that by adding lactic acid bacteria ferment to the vaginal cleansing composition of the present invention, the amount of antibacterial complex can be significantly reduced, for example, from 20% to 10%. Since the unit cost of the antibacterial complex is much higher than the unit cost of the lactic acid bacteria ferment, the vaginal cleansing composition of the present invention has the advantage of lower cost.

[0073] Table 2: Survival rates of different antibacterial components against vaginal pathogens and probiotics

[0074]

[0075] In summary, the vaginal cleansing composition of the present invention comprises an antibacterial complex and a fermentation product obtained by fermenting Streptococcus thermophilus, Lactobacillus salivarius, Lactobacillus rhamnosus, and Bifidobacterium bifidum in a 2:3:3:2 ratio to inhibit vaginitis pathogens and promote the growth of probiotics. Therefore, the vaginal cleansing composition of the present invention can not only inhibit vaginitis pathogens through the antibacterial complex, but also promote the growth of probiotics in the vagina through the lactic acid bacteria fermentation product, thereby avoiding the alteration of the vaginal acidic environment caused by the inhibition of probiotics by the antibacterial complex.

[0076] The present invention has been described in detail above with reference to the embodiments, but the content described is only a preferred embodiment of the present invention and should not be considered as limiting the scope of the present invention. All equivalent changes and improvements made in accordance with the scope of the present invention should still fall within the patent coverage of the present invention.

Claims

1. A vaginal cleansing composition, characterized in that... It includes: Lactic acid bacteria fermentation product is obtained by co-fermentation of at least one of the following: milk, milk powder, whey, casein, soy protein, and collagen, using isolated Streptococcus thermophilus, Lactobacillus salivarius, Lactobacillus rhamnosus, and Bifidobacterium bifidum, with the ratio of Streptococcus thermophilus, Lactobacillus salivarius, Lactobacillus rhamnosus, and Bifidobacterium bifidum being 2:3:3:

2. The antibacterial complex comprises wild soybean seed extract, houttuynia cordata extract, scutellaria baicalensis root extract, neem leaf extract, rehmannia glutinosa root extract, white willow bark extract, phellodendron bark extract, o-cymene-5-ol, pear juice lactobacillus fermentation filtrate, glyceryl caprylate, ethylhexylglycerin, PEG-60 hydrogenated castor oil, glycolic acid, butylene glycol, and water; excipient; The content of lactic acid bacteria fermentation product is 0.4-10%, and the content of antibacterial complex is 5-15%.

2. The vaginal cleansing composition according to claim 1, characterized in that: The antibacterial complex contains 0.006%~0.008% of wild soybean seed extract, 0.014%~0.019% of houttuynia cordata extract, 0.016%~0.02% of scutellaria baicalensis root extract, 0.018%~0.022% of neem leaf extract, 0.038%~0.042% of rehmannia glutinosa root extract, 0.046%~0.052% of willow bark extract, and 0.123%~0. 127% of Phellodendron bark extract, 0.23%–0.27% of O-cymene-5-ol, 0.68%–0.72% of Lactobacillus pear juice fermentation filtrate, 0.8%–1.2% of glyceryl caprylate, 0.8%–1.2% of ethylhexylglycerin, 3.6%–4.2% of PEG-60 hydrogenated castor oil, 5.3%–5.9% of glycolic acid, 38.3%–42.1% of butylene glycol, and water.

3. The vaginal cleansing composition according to claim 1, characterized in that: Lactic acid bacteria fermentation products are fermentation broths containing inactive bacteria or bacteria removed, or dried powders of the fermentation broth.

4. The vaginal cleansing composition according to claim 1, characterized in that: It also includes a thickener, which is a combination of natural glucan gum, carbomer or more, with a thickener content of 0.2-5%.

5. The vaginal cleansing composition according to claim 1, characterized in that: It also includes a first conditioning agent, which is a combination of sodium hyaluronate, an anti-allergy complex, allantoin, or more thereof, and the content of the first conditioning agent is 0.005-5%, wherein the anti-allergy complex contains deep sea water and zucchini seed extract.

6. The vaginal cleansing composition according to claim 1, characterized in that: It also includes a second conditioning agent, which comprises a first complex, a second complex, menthol oil, tea tree oil, aloe vera extract, senna extract, nano silver or a combination thereof, in a content of 0.1-8%, wherein the first complex comprises dipropylene glycol, hydroxyacetophenone, octyl ethylene glycol and dipotassium glycyrrhizate, and the second complex comprises pepper fruit extract, Pulsatilla root extract and moss extract.

7. The vaginal cleansing composition according to claim 1, characterized in that: The excipient contains sodium hydroxide, triethanolamine, glycerol or a combination thereof and water, wherein the content of sodium hydroxide, triethanolamine, glycerol or a combination thereof is 0.05-10%, and the remainder is made up to 100% with water.

8. The vaginal cleansing composition according to claim 1, characterized in that: It can be in the form of tablets, capsules, powders, creams, sprays, gels, solutions, ointments, or lotions.

9. Use of the vaginal cleansing composition according to any one of claims 1 to 8 in the preparation of a composition for inhibiting vaginitis pathogens.

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