A kind of enzymatic small molecule peptide fertilizer composition and preparation method thereof

Abstract

The invention discloses an enzymatically hydrolyzed small molecule peptide fertilizer composition and a preparation method thereof, and relates to the technical field of fertilizers. The fertilizer composition of the invention comprises an enzymatically hydrolyzed small molecule peptide composition, potassium dihydrogen phosphate, monoammonium phosphate, potassium sulfate fertilizer, potassium humate and boron zinc fertilizer. The fertilizer composition of the invention can replace urea and significantly reduce the usage of other fertilizers. Compared with conventional fertilization schemes, the cost is reduced, the benefit is increased, and the purpose of reducing weight and increasing efficiency is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizers, and in particular to an enzymatically hydrolyzed small molecule peptide fertilizer composition and a preparation method thereof. Background Art

[0002] Fertilizer plays a significant role in increasing crop yields and is an indispensable production factor in modern agriculture. Fertilizer plays an important role in increasing the output of agricultural products and ensuring human food supply.

[0003] Amino acids play an important ecological role in the soil. However, the content of amino acids in the soil is extremely small. The addition of amino acids from exogenous sources can make up for the lack of soil demand for these important organic nitrogen sources. Using enzymatic hydrolysis technology to hydrolyze waste materials to prepare amino acid liquid fertilizer, which is applied to agricultural cultivated soil, can not only supplement amino acids in the soil and improve soil quality, but also provide a new way for the development and utilization of waste fish feed and sustainable agricultural development. Amino acids exist in fertilizers as the smallest molecules that make up proteins. They are easily absorbed by crops. They also have the function of improving the disease resistance of fertilized objects and improving the quality of fertilized crops. Supplement essential amino acids for plants, stimulate and regulate rapid plant growth, promote plant growth and promote the absorption of nutrients. Enhance the metabolic function of plants, improve photosynthesis, promote the development of plant roots, and accelerate plant growth and reproduction.

[0004] In addition, the small molecule peptides obtained by enzymatic hydrolysis of animal proteins have extremely strong biological activity and diversity, and have a significant effect on improving the quality and yield of agricultural products. For example, Chinese patent application CN201610252537.6 discloses an enzymatically active small molecule peptide organic water-soluble fertilizer and a production method thereof, which uses enzymatically active small molecule peptides as carriers to compound nitrogen fertilizer, phosphate fertilizer, potash fertilizer, humate, and fulvic acid into the product to form an organic water-soluble fertilizer with strong stability and high effectiveness. The product of the present invention has the effects of regulating crop nutritional growth, enhancing crop resistance, increasing yield, and improving quality, and can improve the utilization rate of fertilizers and soil nutrients.

[0005] However, on the one hand, the current technology for preparing small molecule peptides using animal and plant raw materials is still immature, and the protein extraction and decomposition process is complex and not conducive to operation. On the other hand, there is little research on the promoting effect of the molecular weight distribution of small molecule peptides on crops. Therefore, further research and development is still needed in the field of preparing small molecule peptide crop fertilizers.

[0006] In view of this, in order to address the deficiencies of the prior art, the present invention provides an enzymatically hydrolyzed small molecule peptide fertilizer composition and a preparation method thereof. The small molecule peptide composition is produced from animal and plant raw materials and further prepared into a fertilizer composition, which can significantly promote crop growth. Compared with conventional fertilizers, it has technical effects such as improving efficiency and increasing yield. Summary of the invention

[0007] The purpose of the present invention is to provide an enzymatically hydrolyzed small molecule peptide fertilizer composition and a preparation method thereof, which can significantly improve crop quality, increase crop yield and save costs.

[0008] To achieve the above-mentioned purpose of the invention, the technical solution of the present invention is as follows:

[0009] In one aspect, the present invention provides a fertilizer composition, comprising an enzymatically hydrolyzed small molecule peptide composition, potassium dihydrogen phosphate, monoammonium phosphate, potassium sulfate fertilizer, potassium humate and boron zinc fertilizer, wherein the enzymatically hydrolyzed small molecule peptide composition is prepared according to the following steps:

[0010] (1) crushing the plant raw material, adding water, mixing and boiling, cooling, adding cellulase for enzymolysis, inactivating the enzyme, filtering out the liquid to obtain protein material A; the plant raw material is wheat, sorghum, soybean and sunflower head;

[0011] (2) soaking the animal raw material in sodium bicarbonate solution, filtering, soaking in ethanol, filtering, mixing and beating, adding water, adding lipase for enzymolysis, and inactivating the enzyme to obtain protein material B; the animal raw material is shrimp shells and fish viscera;

[0012] (3) mixing protein material A, protein material B and wood ash uniformly, adding protease and auxiliary agent for enzymolysis, inactivating enzyme, filtering to obtain filtrate, freeze-drying, and pulverizing to obtain soluble small molecule peptide composition powder,

[0013] Wherein, the auxiliary agent is at least one of lignin sulfonate and humate.

[0014] Preferably, the fertilizer composition comprises 300-500 g / mu of enzymatic small molecule peptide composition, 1-3 kg / mu of potassium dihydrogen phosphate, 10-18 kg / mu of monoammonium phosphate, 10-15 kg / mu of potassium sulfate fertilizer, 0.1-1 kg / mu of potassium humate and 500-1000 g / mu of boron zinc fertilizer.

[0015] Further preferably, the fertilizer composition includes 410 g / mu of enzymatic small molecule peptide composition, 2 kg / mu of potassium dihydrogen phosphate, 15 kg / mu of monoammonium phosphate, 12 kg / mu of potassium sulfate fertilizer, 1 kg / mu of potassium humate and 800 g / mu of boron zinc fertilizer.

[0016] Preferably, in step (1), the mass ratio of the wheat, sorghum, soybean and sunflower disc is 1-10:1-10:1-10:1, and more preferably 2:2:2:1.

[0017] Preferably, in step (1), the boiling time is 20-60 min, more preferably 25-35 min.

[0018] Preferably, in step (1), the conditions for enzymolysis of the cellulase are: solid content of 1-5%, enzymolysis time of 30-120 min, enzyme dosage of 0.1-3%, and enzymolysis temperature of 40-60°C. Most preferably, the conditions for enzymolysis of the cellulase are: solid content of 3%, enzymolysis time of 90 min, enzyme dosage of 0.5%, and enzymolysis temperature of 50°C.

[0019] Preferably, in steps (1)-(3), the enzymatic hydrolysis is carried out under stirring.

[0020] Preferably, in step (2), the solid-to-liquid ratio (weight ratio) of the sodium bicarbonate solution is 1:5-20, more preferably 1:10; the soaking time is 2-8h, more preferably 3-4h.

[0021] Preferably, in step (2), the temperature of ethanol immersion is 40-80°C, more preferably 50°C; the solid-liquid ratio (weight ratio) of ethanol immersion is 1:5-20, more preferably 1:10; the time of ethanol immersion is 5-24h, more preferably 8-10h.

[0022] Preferably, in step (2), the conditions for lipase hydrolysis are: solid content 3-10%, hydrolysis time 30-60 min, hydrolysis temperature 25-40° C., enzyme dosage 0.1-5%. Most preferably, the conditions for lipase hydrolysis are: solid content 6%, hydrolysis time 50 min, hydrolysis temperature 30° C., enzyme dosage 0.3%.

[0023] Preferably, the weight ratio of the protein material A to the protein material B is 1:1-3, more preferably 1:1.5. Preferably, in step (3), the protease is alkaline protease and papain, more preferably, the weight ratio of the alkaline protease to papain is 3:1.

[0024] Preferably, in step (3), the conditions for protease hydrolysis are: hydrolysis time 30-120 min, hydrolysis temperature 40-60° C., enzyme dosage 0.1-5%. Most preferably, the conditions for lipase hydrolysis are: hydrolysis time 60 min, hydrolysis temperature 50° C., enzyme dosage 1%.

[0025] Preferably, in step (3), the amount of wood ash added is 1-2.5% of the total weight of protein material A and protein material B, and more preferably 2%.

[0026] Preferably, in step (3), the additive is added in an amount of 0.1-10%, more preferably 3%.

[0027] Preferably, in step (3), the auxiliary agents are lignin sulfonate and humate, and the mass ratio of the two is 1:1-10, and more preferably 1:5.

[0028] Preferably, the above-mentioned enzymatically hydrolyzed small molecule peptide composition includes amino acids and small molecule peptides. Further preferably, the content of the amino acids is 10-15%, and the molecular weight distribution of the small molecule peptides is: below 430, 55-65%; greater than 430 and less than 1700, 29-32%; 1700-5800, not more than 10%.

[0029] On the other hand, the present invention provides a method for preparing the above-mentioned fertilizer composition, comprising the following steps: preparing an enzymatically hydrolyzed small molecule peptide composition according to the above steps, and then mixing the formulated amount of the enzymatically hydrolyzed small molecule peptide composition, potassium dihydrogen phosphate, monoammonium phosphate, potassium sulfate fertilizer, potassium humate and boron zinc fertilizer to obtain the fertilizer composition.

[0030] The beneficial effects of the present invention are:

[0031] (1) The present invention provides an enzymatically hydrolyzed small molecule peptide fertilizer composition, which includes an enzymatically hydrolyzed small molecule peptide composition. The fertilizer composition of the present invention can replace urea and significantly reduce the use of other fertilizers. Compared with conventional fertilization schemes, it reduces costs and increases benefits, thereby achieving the purpose of reducing weight and increasing efficiency.

[0032] (2) The preparation method of the enzymatically hydrolyzed small molecule peptide composition is simple and easy. The animal raw materials and the plant raw materials are processed separately, and then a certain amount of wood ash is added to the protein material for co-enzymatic hydrolysis, which can improve the enzymatic hydrolysis efficiency of the protease, save the amount of protease used, and improve the enzymatic hydrolysis effect.

[0033] (3) Compared with single enzymatic hydrolysis, the combined enzymatic hydrolysis of alkaline protease and papain improves the enzymatic hydrolysis effect, significantly increases the amino acid content, and improves the distribution of small molecule peptides.

[0034] (4) The present invention uses lignin sulfonate and humate as enzymatic hydrolysis aids, which, in addition to adjusting the pH, are also beneficial to increasing the amino acid content. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 The present invention is a process flow chart for preparing the small molecule peptide composition.

[0036] Figure 2 This is a diagram showing the effect of the small molecule peptide composition of the present invention on the development of corn seeds.

[0037] Figure 3 This is a diagram showing the effect of the small molecule peptide composition of the present invention on the development of the root system of cotton seedlings.

[0038] Figure 4 This is a graph showing the effects of different fertilization treatments on the number of true leaves, fruit branches, buds and bolls, and plant height at the cotton seedling stage.

[0039] Figure 5This is a diagram showing the effects of different fertilization treatments on agronomic traits of cotton at the boll stage (Korla).

[0040] Figure 6 This is a graph showing the effect of different fertilizations on cotton dry matter accumulation.

[0041] Figure 7 This is a graph showing the effects of different fertilization treatments on the photosynthetic performance of cotton. DETAILED DESCRIPTION

[0042] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of protection claimed in this application, and those skilled in the art can make various changes and modifications to the invention of this application based on the disclosed content, and they should also fall within the scope of protection claimed in this application.

[0043] The present invention is further described below in the form of specific embodiments. The various chemical reagents used in the embodiments of the present invention are obtained through conventional commercial channels unless otherwise specified. Unless otherwise specified, the contents (percentages) described below are all mass contents (mass percentages). Unless otherwise specified, it is understood that it is carried out at room temperature. Hereinafter, the term "solid content" refers to the mass percentage of the solid part in the whole mixture, and the amount of enzyme added below is the mass percentage of the weight of the enzyme to be enzymolyzed raw material, such as "0.3% lipase" represents that the mass of lipase accounts for 0.3% of the total mass of plant raw materials, and "1% composite protease" represents that the mass of composite protease accounts for 0.3% of the total mass of protein material A and protein material B. The amount of auxiliary agent added is calculated in the same way as the amount of enzyme added. In the following examples, the concentration of sodium bicarbonate solution is 1%.

[0044] In the embodiments of the present invention, the cellulase used is Xiasheng cellulase FFG-0665; the lipase used is Xiasheng lipase SDG-2426, the alkaline protease used is Xiasheng alkaline protease FDG-2202, and the papain used is Xiasheng papain FDG-2203, which does not limit the scope of protection of the present invention.

[0045] Example 1

[0046] (1) Wheat, sorghum, soybean and sunflower disc are mixed and crushed, mixed in a mass ratio of 2:2:2:1, water is added to adjust the solid content to 3%, mixed, boiled, cooled after 30 minutes, cooled to 50° C., maintained at a constant temperature, 0.5% cellulase is added while stirring and enzymolysis is performed for 90 minutes, the temperature is raised to inactivate the enzyme, and the liquid is filtered to obtain protein material A;

[0047] (2) Shrimp shells and fish viscera were mixed in a mass ratio of 1:1, and sodium bicarbonate solution was added and soaked at room temperature for 3 hours, with a solid-liquid ratio (weight ratio) of 1:10. After soaking, the mixture was filtered, washed with water for 3 times, and an equal weight of 75% ethanol was added again, and the mixture was soaked at 50° C. for 10 hours, filtered, mixed and slurried, and water was added to adjust the solid content to 6%. The temperature was maintained at 30° C., 0.3% lipase was added for enzymatic hydrolysis for 50 minutes, and the enzyme was inactivated by heating to obtain protein material B;

[0048] (3) Protein material A and protein material B are mixed in a weight ratio of 1:1.5, 2% wood ash is added and mixed evenly, the temperature is maintained at 50°C, 1% composite protease (0.75% alkaline protease + 0.25% papain), 0.5% potassium lignin sulfonate, and 2.5% potassium humate are added, stirred evenly and enzymolyzed for 60 minutes under stirring, the temperature is raised to inactivate the enzyme, filtered, the filtrate is collected, freeze-dried, and crushed to obtain a soluble small molecule peptide composition powder.

[0049] Example 2

[0050] (1) Wheat, sorghum, soybean and sunflower disc are mixed and crushed, mixed in a mass ratio of 2:2:2:1, water is added to adjust the solid content to 5%, mixed, boiled, cooled after 30 minutes, cooled to 50° C., maintained at a constant temperature, 0.5% cellulase is added while stirring and enzymolysis is performed for 70 minutes, the temperature is raised to inactivate the enzyme, and the liquid is filtered to obtain protein material A;

[0051] (2) Shrimp shells and fish viscera were mixed in a mass ratio of 1:1, and sodium bicarbonate solution was added and soaked at room temperature for 4 hours, with a solid-liquid ratio (weight ratio) of 1:15. After soaking, the mixture was filtered, washed with water for 3 times, and an equal weight of 75% ethanol was added again, and the mixture was soaked at 50° C. for 8 hours, filtered, mixed and slurried, and water was added to adjust the solid content to 3%. The temperature was maintained at 30° C., 1% lipase was added for enzymatic hydrolysis for 50 minutes, and the enzyme was inactivated by heating to obtain protein material B;

[0052] (3) Protein material A and protein material B are mixed in a weight ratio of 1:3, 1% wood ash is added and mixed evenly, the temperature is maintained at 50°C, 1% composite protease (0.75% alkaline protease + 0.25% papain), 1.0% potassium lignin sulfonate, and 2.0% potassium humate are added, stirred evenly and enzymolyzed under stirring for 80 minutes, the temperature is raised to inactivate the enzyme, filtered, the filtrate is collected, freeze-dried, and crushed to obtain a soluble small molecule peptide composition powder.

[0053] The amino acid content was tested according to NY 1429-2010, and the results are as follows:

[0054]

[0055]

[0056] It can be seen that the free amino acid content in the compositions prepared in Example 1 and Example 2 is both above 10%.

[0057] The molecular weight distribution of peptides was detected by liquid chromatography according to Appendix A of GB / T 22492-2008. Standard substances included cytochrome C, insulin, bacitracin, tetrapeptide glycine-glycine-tyrosine-arginine, and glycine-glycine-glycine. The chromatographic column was TSKgelG2000SWXL (7.8 mm × 30 cm). The mobile phase was acetonitrile: water: trifluoroacetic acid (20:80:0.1). The flow rate was 0.5 ml / min, the column temperature was 35 °C, and the injection volume was 10 μl.

[0058] The calculation formula is:

[0059] X=(A i / A 总 )×100%

[0060] X: Relative percentage of each molecular weight segment

[0061] Ai: area of ​​each molecular weight segment

[0062] A 总 : Total area of ​​total molecular weight segment

[0063] The results of Example 1 are as follows:

[0064]

[0065] The results of Example 2 are as follows:

[0066]

[0067] Comparative Example 1

[0068] (1) wheat, sorghum, soybeans and sunflower heads are mixed and crushed in a mass ratio of 2:2:2:1, water is added to adjust the solid content to 3%, mixed evenly, boiled, cooled after 30 minutes, filtered, and the liquid is removed to obtain a mixture A;

[0069] (2) Shrimp shells and fish viscera were mixed in a mass ratio of 1:1, and soaked in sodium bicarbonate solution at room temperature for 3 hours, with a solid-liquid ratio (weight ratio) of 1:10. After soaking, the mixture was filtered, washed with water for 3 times, and an equal weight of 75% ethanol was added again, soaked at 50° C. for 10 hours, filtered, mixed and beaten to obtain a mixture B;

[0070] (3) Mix mixture A and mixture B in a weight ratio of 1:1.5, add 2% wood ash and mix evenly, maintain the temperature at 50°C, add 1% composite protease (0.75% alkaline protease + 0.25% papain), 0.5% potassium lignin sulfonate, and 2.5% potassium humate, stir evenly and perform enzymolysis for 60 minutes under stirring, heat up to inactivate the enzyme, filter, collect the filtrate, freeze-dry, and crush to obtain a soluble small molecule peptide composition powder.

[0071] Comparative Example 2

[0072] The difference from Example 1 is that only 1% alkaline protease is added during the enzymatic hydrolysis in step (3), and the conditions of the other steps are the same.

[0073] Comparative Example 3

[0074] The difference from Example 1 is that in step (3), potassium lignin sulfonate is not added as an auxiliary agent, but only 2.5% potassium humate is added, and the conditions of the other steps are the same.

[0075] Comparative Example 4

[0076] The difference from Example 1 is that no wood ash is added in step (3), and the conditions of the remaining steps are the same.

[0077] The amino acid content and peptide molecular weight distribution detection methods are the same as before, and the results are as follows:

[0078] Project name (content) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Threonine, % 0.18 0.29 0.45 0.31 Serine, % 0.71 1.26 1.01 0.96 Glutamic acid, % 0.96 1.05 1.03 0.93 Glycine, % 0.81 0.97 0.86 0.99 Alanine, % 0.43 0.56 0.50 0.64 Cysteine, % - - 0.02 0.01 Valine, % 0.43 0.26 0.52 0.55 Methionine, % 0.04 0.19 0.08 0.07 Isoleucine, % 0.28 0.25 0.33 0.31 Leucine, % 0.37 0.40 0.53 0.43 Tyrosine, % 0.08 0.05 0.07 0.12 Phenylalanine, % 0.66 0.41 0.82 0.76 Histidine, % 0.17 0.47 0.36 0.31 Lysine, % 0.43 0.52 0.35 0.67 Arginine, % 0.49 0.65 0.67 0.70 Proline, % 0.53 0.36 0.77 0.83 Total free amino acids, % 6.59 7.70 8.38 8.61

[0079] As can be seen from the table above, the amino acid content of Comparative Examples 1-4 did not reach 10%.

[0080] The molecular weight distribution of the peptide of Comparative Example 1 is as follows:

[0081]

[0082] The molecular weight distribution of the peptide of Comparative Example 2 is as follows:

[0083]

[0084]

[0085] The molecular weight distribution of the peptide of Comparative Example 3 is as follows:

[0086]

[0087] The molecular weight distribution of the peptide of Comparative Example 4 is as follows:

[0088]

[0089] Experimental Example 1

[0090] The root development of corn seeds and cotton seedlings was cultivated and the results are shown in the attached figure. Figure 2 , 3 Test group: The composition of Example 1 of the present invention was prepared into a 0.1% aqueous solution to soak corn seeds; the composition of Example 1 was dripped on cotton seedlings;

[0091] Control group: corn seeds were soaked in water only; cotton seedlings were not subjected to the composition solution, and other culture conditions were the same as those of the test group.

[0092] From the attached Figure 2 , 3 It can be seen that, compared with the control group, the composition of the present invention can significantly increase the germination rate of corn seeds and promote the development of the root system of cotton seedlings.

[0093] Experimental Example 2

[0094] 2.1 Test location:

[0095] Huyanghe Experiment Station of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences (44°44′N, 84°48′E, temperate continental arid climate, average annual precipitation of 182.1 mm, medium soil fertility, and clay loam) and Korla Experiment Station (41°10′N, 85°14′E, temperate continental arid climate, average annual precipitation of 58.6 mm, medium soil fertility, slightly saline-alkali, and clay loam).

[0096] Experimental varieties: China Cotton Research Institute 109 and China Cotton Research Institute 99, the fiber quality of both are above "double 30".

[0097] 2.2 Test methods:

[0098] (1) Huyanghe Experimental Station

[0099] The fertilizer test was designed in a randomized block, with two treatments, namely CK control (conventional field fertilization) and T1 treatment (using the composition of Example 1 of the present invention to replace urea). The "Kuan Zaoyou" mode (1 film, 3 rows, equal row spacing) was adopted, and the planting density was 12,000 plants / mu. The fertilization measures for the CK treatment were carried out in accordance with the experimental field management. The nitrogen fertilizers were urea and monoammonium, the phosphate fertilizer was monoammonium phosphate, and the potash fertilizer was potassium sulfate. Urea was not applied in the T1 treatment, and the composition of Example 1 was used instead, and drip application was carried out according to the fertilization plan. Other field management measures such as weeding, pest control, and topping were carried out in accordance with the management of the experimental station. Each treatment was set up with 3 replications, with a total of 6 plots, and the area of ​​each plot was 57m×6.5m=370.5m 2 , with a total area of ​​3.3 acres, and sowing was carried out on April 24, 2020.

[0100] (2) Korla Experimental Station

[0101] A large-area demonstration comparison was adopted, and 67 mu of test land was selected. There were two water outlets (independent irrigation systems) for fertilization dripping, of which 37 mu was for field treatment (CK), and 30 mu was treated with the composition of Example 1 instead of urea (T1) according to the fertilization plan. Other field management measures such as weeding, pest control, topping, etc. were carried out according to the management of the experimental station.

[0102] Fertilization plan:

[0103] Huyanghe CK group: 42 kg / mu of urea + 26 kg / mu of monoammonium + 3 kg / mu of potassium dihydrogen phosphate + 20 kg / mu of potassium sulfate + 20 kg / mu of monoammonium phosphate + 1 kg / mu of potassium humate + 800 g / mu of boron zinc fertilizer;

[0104] Korla CK group: 42 kg / mu of urea + 20 kg / mu of monoammonium + 3 kg / mu of potassium dihydrogen phosphate + 20 kg / mu of potassium sulfate + 14.5 kg / mu of monoammonium phosphate + 2 kg / mu of potassium humate + 800 g / mu of boron zinc fertilizer + 9.3 kg / mu of organic fertilizer;

[0105] Group T1: 410 g / mu of the composition of Example 1 + 2 kg / mu of potassium dihydrogen phosphate + 15 kg / mu of monoammonium phosphate + 12 kg / mu of potassium sulfate fertilizer + 1 kg / mu of potassium humate + 800 g / mu of boron zinc fertilizer.

[0106] 2.3 Survey indicators:

[0107] (1) Records of cotton disease resistance and cultivation practices

[0108] Record in detail the field management such as fertilization time and amount, dripping time and amount of water, spraying time and dosage, and calculate the output-input ratio.

[0109] (2) Investigation of agronomic traits of cotton

[0110] During the cotton growth period, the survey was conducted six times (seedling stage, bud stage, initial flowering stage, full flowering stage, boll stage, and boll opening stage) to survey 10 fixed-plant cotton plants in each plot. The recorded contents mainly included: plant height, number of fruit branches, and the number of buds, flowers, bolls, rotten bolls, and boll opening on each fruit branch.

[0111] (3) Determination of cotton photosynthetic performance and light energy utilization

[0112] During the growth period of cotton, photosynthetic efficiency and light energy utilization rate of different treatments were measured using photosynthetic meter Li-6800 and light meter Li-300.

[0113] (4) Cotton yield, composition, and fiber quality

[0114] The theoretical and actual yields were measured during the boll opening period. 2The yield was measured in the plots for 3 times, and the fiber quality was tested at the same time. The economic benefits of seed cotton in the T1 group were compared.

[0115] 2.4 Results

[0116] (1) Effects of different fertilization treatments on agronomic shape of cotton

[0117] Dynamic changes of cotton agronomic traits under different fertilization treatments Figure 4 , Figure 5 As shown, in the Huyanghe Experimental Station, there was no significant difference in plant height between the two treatments in the early growth stage. As the growth process progressed, the T1 treatment played a significant role in promoting the growth of plant height. At 60d (bud stage) and 90d (boll stage) after emergence, the plant height of the T1 treatment was 10% and 12% higher than that of CK. 20-90d after emergence, the plant height growth of CK and T1 treatments was 62cm and 72cm respectively, indicating that T1 promoted the nutritional growth of cotton. For the number of true leaves in the seedling stage, T1 was higher than CK. 70D after sowing, T1 treatment was 22.2% higher than CK, and the number of fruit branches was 3.8% higher. 101d after sowing (full boll stage), the number of bolls per plant under T1 treatment was 2.3 higher than CK, and the number of fruit branches was 1.8 higher. Similarly, at the Korla Experimental Station, the above shapes all showed T1>CK. The test results of the two places showed that the composition of the present invention played a good role in promoting the growth and development of cotton.

[0118] (2) Effects of different fertilization treatments on cotton dry matter weight

[0119] The accumulation rules of cotton dry matter under different fertilization treatments are as follows Figure 6 As shown, the distribution of dry matter and nitrogen in the early stage is centered on the vegetative organs, and after the flowering and bolling stage, the distribution of dry matter and nitrogen gradually shifts to the reproductive organs, and finally the proportion of dry matter in the reproductive organs is relatively high. In the cotton seedling stage, the proportion of photosynthetic products distributed to the stems is the highest. From the budding to the initial flowering stage, only 6-9% is distributed to the buds and bolls, and it gradually increases in the later stage. After entering the boll opening stage, most of the dry matter (61-63%) is distributed to the bolls. During the entire growth period, the weight of leaves, stems, reproductive organs and total dry matter showed that T1>CK, especially in the full boll stage (August 9), the weight of reproductive organs and total dry matter treated with T1 was 43.5% and 54.3% higher than that of CK, indicating that the application of the composition of the present invention can effectively increase the proportion of photosynthetic products converted to reproductive organs and accelerate the construction of cotton dry matter.

[0120] (3) Effects of different fertilization treatments on cotton photosynthetic performance

[0121] The photosynthetic performance of cotton under different fertilization treatments was analyzed ( Figure 7), the results showed that the T1 treatment had a significant effect on the net photosynthetic rate, transpiration rate, intercellular carbon dioxide concentration and stomatal conductance of cotton at each growth stage, all of which were higher than those of the CK treatment. Especially in the full-blooming stage, the net photosynthetic rate, transpiration rate, intercellular carbon dioxide concentration and stomatal conductance of the T1 treatment were 3.5%, 13.9%, 5.6% and 21.6% higher than those of the CK treatment, respectively.

[0122] (4) Effects of different fertilization treatments on cotton light energy utilization

[0123] The results are as follows:

[0124]

[0125] As can be seen from the table above, the light absorption rate under T1 treatment was significantly higher than that under CK, and the transmittance was significantly lower than that under CK. This indicates that the T1 group can form a reasonable canopy structure, promote the absorption and utilization of light energy by spiders, and enhance the photosynthetic efficiency of cotton populations.

[0126] (5) Effects of different fertilization treatments on cotton yield and fiber quality

[0127] Impact on production:

[0128]

[0129] Impact on fiber quality:

[0130]

[0131] As can be seen from the table above, there is no significant difference between the two treatments for fiber quality, indicating that the composition of the present invention does not affect fiber quality. Compared with CK, T1 treatment significantly increases the number of bolls per plant. Huyanghe T1 increases yield by 5.6% over CK, and Korla T1 increases yield by 4.7% over CK. Based on the seed cotton purchase price of 6.5 yuan / kg, the yield per mu increases by about 130 yuan.

[0132] (6) Effects of different fertilization treatments on economic benefits

[0133] Through statistics of fertilizer input and output value, the output-input ratio was calculated. The output-input ratio of the Populus euphratica CK group was 10.5, that of the T1 group was 12.5, that of the Korla CK group was 15, and that of the T1 group was 16.2. By using the composition of the present invention, the fertilizer input cost was reduced by 6-22 yuan / mu, and the net income was increased by about 139 yuan / mu.

[0134] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A fertilizer composition, characterized in that The invention comprises an enzymatically hydrolyzed small molecule peptide composition, potassium dihydrogen phosphate, monoammonium phosphate, potassium sulfate fertilizer, potassium humate and boron zinc fertilizer, wherein the enzymatically hydrolyzed small molecule peptide composition is prepared according to the following steps: (1) After the plant raw materials are crushed, water is added to mix and boiled, the mixture is cooled, cellulase is added to hydrolyze, the enzyme is inactivated, and the liquid is filtered to obtain protein material A; the plant raw materials are wheat, sorghum, soybean and sunflower heads; (2) Soak the animal raw materials in sodium bicarbonate solution, filter, soak in ethanol, filter, Mix and beat, add water, add lipase for enzymolysis, and inactivate the enzyme to obtain protein material B; the animal raw materials are shrimp shells and fish viscera; (3) Mixing protein material A, protein material B and wood ash uniformly, adding protease and auxiliary agent for enzymolysis, inactivating enzyme, filtering to obtain filtrate, freeze-drying, and pulverizing to obtain soluble small molecule peptide composition powder, in, In step (1), the conditions for cellulase hydrolysis are: solid content of 1-5%, hydrolysis time of 30-120 min, enzyme dosage of 0.1-3%, and hydrolysis temperature of 40-60°C; in step (2), the conditions for lipase hydrolysis are: solid content of 3-10%, hydrolysis time of 30-60 min, hydrolysis temperature of 25-40°C, and enzyme dosage of 0.1-5%; in step (3), the conditions for protease hydrolysis are: hydrolysis time of 30-120 min, hydrolysis temperature of 40-60°C, and enzyme dosage of 0.1-5%; In step (3), the weight ratio of the protein material A to the protein material B is 1:1-3, the protease is alkaline protease and papain, and the weight ratio of alkaline protease to papain is 3:1; The amount of the plant ash added is 1-2.5% of the total weight of the protein material A and the protein material B, the amount of the auxiliary agent added is 0.1-10%, and the auxiliary agents are lignin sulfonate and humate, and the mass ratio of the two is 1:1-10.

2. The fertilizer composition according to claim 1, characterized in that In step (1), the mass ratio of the wheat, sorghum, soybean and sunflower disc is 1-10:1-10:1-10:

1.

3. The fertilizer composition according to claim 1, characterized in that In step (1), the boiling time is 20-60 minutes.

4. The fertilizer composition according to claim 1, characterized in that In step (2), the solid-liquid ratio of the sodium bicarbonate solution is 1:5-20, the soaking time is 2-8 hours, the ethanol soaking temperature is 40-80°C, the solid-liquid ratio of the ethanol soaking is 1:5-20, and the ethanol soaking time is 5-24 hours.

5. The fertilizer composition according to claim 1, characterized in that The invention comprises 300-500 g / mu of enzymatic small molecule peptide composition, 1-3 kg / mu of potassium dihydrogen phosphate, 10-18 kg / mu of monoammonium phosphate, 10-15 kg / mu of potassium sulfate fertilizer, 0.1-1 kg / mu of potassium humate and 500-1000 g / mu of boron zinc fertilizer.

6. The fertilizer composition according to claim 5, characterized in that It includes 410g / mu of enzymatic small molecule peptide composition, 2kg / mu of potassium dihydrogen phosphate, 15kg / mu of monoammonium phosphate, 12kg / mu of potassium sulfate fertilizer, 1kg / mu of potassium humate and 800g / mu of boron zinc fertilizer.

7. The method for preparing the fertilizer composition according to any one of claims 1 to 6, characterized in that: The following steps are involved: The enzymatically hydrolyzed small molecule peptide composition is prepared, and then the enzymatically hydrolyzed small molecule peptide composition, potassium dihydrogen phosphate, monoammonium phosphate, potassium sulfate fertilizer, potassium humate and boron zinc fertilizer in a prescribed amount are mixed to obtain the product.

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