A multi-channel magnetic attraction type colorimetric detection device and method

Abstract

This invention discloses a multi-channel magnetic colorimetric detection device and method. The device includes a light source, a dark chamber, a photographic device, a concentration gradient color chart, and one or more cuvettes. The method includes the following steps: 1) Using the photographic device, acquire images of the concentration gradient color chart and establish a standard curve of color information versus concentration. 2) Place the sample to be colorimetrically detected into the cuvette. Open the movable cover of the sample chamber, then place the cuvette into the slot, and close the movable cover to seal the sample chamber. 3) Attach the photographic device to the photographic panel, and align the camera of the photographic device with the camera hole. 4) Use the photographic device to acquire images of the sample inside the sample chamber, obtain the color information of the sample image, and determine the sample concentration based on the standard curve of color information versus concentration. This invention solves the problems of photographic device placement and color differences between different photographic devices.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of colorimetric detection, and particularly relates to a multi-channel magnetic colorimetric detection device and method. BACKGROUND

[0002] POCT is a detection method that uses portable analysis instruments and supporting reagents to obtain results quickly on the sampling site. With the progress of science and technology and the development of society, people's concern for their own health is increasing, which makes the traditional detection method unable to meet the demand of people for health monitoring in all aspects under the new situation. POCT is favored and widely used in clinical testing, major epidemic detection, food safety monitoring, and public health field due to its advantages of rapidity, portability, low cost, and no need for professional technicians, and has become a research hotspot in recent years. Smartphones can be used as portable analysis instruments in POCT, and various POCT technologies based on smartphones have been developed, such as smartphone-based fluorescence detection, smartphone-based electrochemical detection, and smartphone-based colorimetric detection.

[0003] Smartphone-based colorimetric detection uses the visualized results to take pictures of the sample to be tested using a smartphone, and analyzes the pictures using a color space model. With the powerful computing advantage of the smartphone, the relationship between color information and sample concentration can be given in real time, and the detection of unknown sample concentration can be realized. This method does not require additional equipment and complex operation process, so it has attracted widespread attention in recent years.

[0004] However, there are three key problems in the use of smartphone-based colorimetric detection. The first problem is that in order to ensure the repeatability of the detection, the smartphone needs to be fixed at a certain distance when taking pictures, and the operator needs to free his hands to take the sample to be tested. Therefore, some inventors have designed a smartphone card slot on the picture-taking dark box to fix the smartphone. However, due to the fixed shooting hole, even with the design of the card slot, it is difficult to adapt to different sizes of smartphones. The second problem is that the brightness of the light during the picture-taking will affect the color information of the picture, and thus greatly affect the repeatability and accuracy of the results. The design of the dark box can solve the problem of the influence of environmental light on the picture-taking, but how to ensure the stability of the light source in the dark box is still a problem to be solved. The third problem is that the color information of the pictures taken by different smartphones under the same light of the same sample is very different, and it is difficult to solve this problem through color calibration, which is also a key problem that makes smartphone-based colorimetric detection difficult to market. SUMMARY

[0005] The purpose of the present application is to provide a multi-channel magnetic colorimetric detection device, which comprises a light source device, a dark box, a picture-taking device, a concentration gradient color card, and one or more colorimetric cuvettes.

[0006] The light source device provides light for the dark box.

[0007] The dark box comprises a box body, a sample chamber and a photographing panel.

[0008] The sample chamber is a cavity with an open upper end and is located inside the box body.

[0009] One or more clamping grooves are arranged in the sample chamber. The clamping grooves are used for placing cuvettes.

[0010] A movable cover plate is arranged above the sample chamber.

[0011] The photographing panel is arranged on the side wall of the dark box and faces the sample chamber.

[0012] The photographing panel is provided with a camera hole.

[0013] The photographing panel is used for adsorbing a photographing device.

[0014] The cuvettes are used for containing samples to be detected.

[0015] The concentration gradient color card is attached to the inner wall of the sample chamber.

[0016] The concentration gradient color card can be removed.

[0017] The photographing device collects images of the concentration gradient color card and establishes a standard curve of color information and concentration.

[0018] During colorimetric detection, the photographing device is attached to the photographing panel in a magnetic manner, and the camera of the photographing device is aligned with the camera hole.

[0019] The photographing device collects images of the sample in the sample chamber, obtains color information of the sample images, and determines the concentration of the sample according to the standard curve of color information and concentration.

[0020] Further, the multi-channel magnetic colorimetric detection device has two working states.

[0021] The first working state is that the concentration gradient color card is attached to the inner wall of the sample chamber.

[0022] The second working state is that the concentration gradient color card is not attached to the inner wall of the sample chamber, but the cuvette contains a sample to be detected.

[0023] Further, the material of the soft light plate comprises a translucent material.

[0024] The concentration gradient color card comprises a translucent material.

[0025] The material of the dark box comprises an opaque material.

[0026] Furthermore, the light source device includes an LED array, a control unit, and a diffuser.

[0027] The control unit controls the LED array to emit light.

[0028] The light emitted by the LED array passes through the diffuser and then illuminates the sample chamber.

[0029] Furthermore, the light source device also includes a switch.

[0030] The switch is used to control the operation of the light source device.

[0031] Furthermore, the light source device also includes a Type-C connector.

[0032] An external power supply powers the LED array and control unit via a Type-C connector.

[0033] Furthermore, the camera device includes a mobile phone and a processing module mounted on the mobile phone.

[0034] The mobile phone uses its built-in camera to capture images of the concentration gradient color chart and samples inside the sample chamber.

[0035] The processing module establishes a standard curve of color information and concentration based on the image of the concentration gradient color chart.

[0036] The processing module acquires the color information of the sample image and determines the sample concentration based on the standard curve of color information versus concentration.

[0037] Furthermore, the mobile phone communicates with the control unit via Bluetooth.

[0038] The mobile phone detects the brightness of the LED array and transmits a control signal to the control unit, thereby controlling the brightness of the LED array.

[0039] Furthermore, the light source device and the dark box are connected by a magnetic material.

[0040] Furthermore, the material of the camera panel includes a magnetic material.

[0041] Furthermore, the concentration gradient color chart is a color image of the sample under test at different standard concentrations, with one concentration gradient color chart corresponding to one substance.

[0042] The concentration gradient color chart includes multiple color regions. Different color regions have different color information. Each color region is labeled with a corresponding sample concentration value.

[0043] The color information includes red, green, blue, hue, saturation, brightness, lightness, cyan, magenta, yellow, black, and algebraic combinations of the above color information.

[0044] A detection method for a multi-channel magnetic colorimetric detection device includes the following steps:

[0045] 1) Use a camera to capture images of the concentration gradient color chart and establish a standard curve of color information and concentration.

[0046] 2) Place the sample to be tested for colorimetry into the cuvette.

[0047] Open the movable cover of the sample chamber, then place the cuvette into the slot, and close the movable cover to seal the sample chamber.

[0048] 3) Attach the photographing device to the photographing panel and align the camera of the photographing device with the video hole.

[0049] 4) Use the photographing device to capture sample images in the sample chamber, obtain color information of the sample images, and determine the sample concentration based on the standard curve of color information and concentration.

[0050] The technical effects of this invention are undeniable. This invention solves the problem of light source brightness control by detecting and adjusting the light source brightness in real time through a mobile phone, solves the problem of mobile phone placement by setting up a magnetic panel to attach the mobile phone, and solves the problem of color differences in photos taken by different mobile phones by creating a solution standard color card.

[0051] The camera panel of this device is made of magnetic material, allowing mobile phones to be attached to it without the restriction of a SIM card slot. Different mobile phones can be freely attached to the shooting surface for shooting. The magnetic method allows the mobile phone to be tightly attached to the shooting hole, preventing light from entering from the side, while freeing up your hands and making testing more convenient and faster.

[0052] In this invention, the light source and the dark box are designed separately and connected by magnetic attraction to facilitate the installation and replacement of faulty LED chips and internal control circuits.

[0053] The control circuit of the light source in this invention establishes Bluetooth communication with the mobile phone. The brightness of the light source is detected by the mobile phone and adjusted in real time to keep it consistent, ensuring the uniformity of illumination between different mobile phones and different measurements, and solving the problem of the influence of different illumination on the test results. Attached Figure Description

[0054] Figure 1 The external structure of the multi-channel magnetic colorimetric detection device;

[0055] Figure 2 This describes the internal structure of a multi-channel magnetic colorimetric detection device.

[0056] Figure 3 Flowchart for the use of a multi-channel magnetic colorimetric detection device;

[0057] Figure 4The results are for colorimetric detection of liquid formaldehyde based on gold nanoparticles. (A) Color chart showing the change in color of gold nanoparticles with formaldehyde concentration; (B) Detection results from this device; (C) Detection results from a spectrophotometer.

[0058] In the diagram: 1. Light source device; 2. Dark box; 3. Concentration gradient color chart; 4. Control unit; 5. Soft light plate; 6. Switch; 7. Type-C connector; 8. Sample chamber; 9. Photo panel; 10. Card slot; 11. Cover plate; 12. Camera hole; 13. Cuvette. Detailed Implementation

[0059] The present invention will be further described below with reference to embodiments, but it should not be construed that the scope of the present invention is limited to the following embodiments. Various substitutions and modifications made based on ordinary technical knowledge and common practices in the art without departing from the above-described technical concept of the present invention should be included within the scope of protection of the present invention.

[0060] Example 1:

[0061] See Figures 1 to 4 A multi-channel magnetic colorimetric detection device includes a light source device 1, a dark box 2, a photographic device, a concentration gradient color card 3, and one or more cuvettes 13.

[0062] The light source device 1 provides illumination to the dark box 2.

[0063] The darkroom 2 includes a box body, a sample chamber 8, and a photographic panel 9.

[0064] The sample chamber 8 is arranged inside the box.

[0065] The sample chamber 8 is provided with one or more slots 10. The slots 10 are used to place cuvettes 13.

[0066] A movable cover plate 11 is provided above the sample chamber 8.

[0067] The photographing panel 9 is located on the side wall of the darkroom 2 and is opposite to the sample chamber 8.

[0068] The camera panel 9 has a camera hole 12.

[0069] The cuvette 13 contains the sample to be tested for colorimetry.

[0070] The imaging device captures images of the concentration gradient color chart 3 and establishes a standard curve of color information and concentration.

[0071] During colorimetric testing, the photographing device is magnetically attached to the photographing panel 9, and the camera of the photographing device is aligned with the camera hole 12.

[0072] The imaging device captures images of the samples in the sample chamber 8, obtains the color information of the sample images, and determines the sample concentration based on the standard curve of color information and concentration.

[0073] Furthermore, the multi-channel magnetic colorimetric detection device has two working states;

[0074] The first working state is: the concentration gradient color card 3 is attached to the inner wall of the sample chamber 8;

[0075] The second working state is: the concentration gradient color card 3 is not attached to the inner wall of the sample chamber 8, but the cuvette 13 contains the sample to be tested.

[0076] Furthermore, the material of the diffuser 5 includes a translucent material;

[0077] The concentration gradient color chart 3 includes a semi-transparent material;

[0078] The material of the dark box 2 includes opaque materials.

[0079] The light source device 1 includes an LED array, a control unit 4, and a diffuser 5.

[0080] The control unit 4 controls the LED array to emit light.

[0081] The light emitted by the LED array passes through the diffuser 5 and then illuminates the sample chamber 8.

[0082] The light source device 1 also includes a switch 6.

[0083] The switch 6 is used to control the operation of the light source device 1.

[0084] The light source device 1 also includes a Type-C connector 7.

[0085] An external power supply powers the LED array and control unit 4 via Type-C connector 7.

[0086] The camera device includes a mobile phone and a processing module mounted on the mobile phone.

[0087] The mobile phone uses its built-in camera to capture images of the concentration gradient color chart 3 and the sample images in the sample chamber 8.

[0088] The processing module establishes a standard curve of color information and concentration based on the image of the concentration gradient color chart 3.

[0089] The processing module acquires the color information of the sample image and determines the sample concentration based on the standard curve of color information versus concentration.

[0090] The mobile phone communicates with the control unit 4 via Bluetooth.

[0091] The mobile phone detects the brightness of the LED array and transmits a control signal to the control unit 4, thereby controlling the brightness of the LED array.

[0092] The light source device 1 and the dark box 2 are connected by magnetic material.

[0093] The material of the camera panel includes magnetic material.

[0094] The color information includes red, green, blue, hue, saturation, brightness, lightness, cyan, magenta, yellow, black, and algebraic combinations of the above color information.

[0095] A detection method for a multi-channel magnetic colorimetric detection device includes the following steps:

[0096] 1) Use a camera to capture images of the concentration gradient color chart 3 and establish a standard curve of color information and concentration.

[0097] 2) Place the sample to be tested for colorimetry into cuvette 13.

[0098] Open the movable cover 11 of the sample chamber 8, then place the cuvette 13 into the slot 10, and close the movable cover 11 to seal the sample chamber 8.

[0099] 3) Attach the photographing device to the photographing panel 9 and align the camera of the photographing device with the camera hole 12.

[0100] 4) Use the photographing device to capture sample images in sample chamber 8, obtain color information of the sample images, and determine the sample concentration based on the standard curve of color information and concentration.

[0101] Example 2:

[0102] A multi-channel magnetic colorimetric detection device includes a light source, a dark chamber, a photographing device, and a concentration gradient color chart. The light source provides stable illumination for mobile phone photography and consists of an LED array and control unit, a diffuser, a switch, and a Type-C connector. The control unit establishes Bluetooth communication with the mobile phone, allowing the phone to program and control the brightness of the LED array via its camera. The dark chamber includes a sample chamber and a photographing panel. The sample chamber has three slots and a cover. The photographing panel has a camera hole in the center and is made of magnetic material, allowing the mobile phone to be firmly attached to it. The light source and dark chamber are connected by magnetic material, facilitating the installation and maintenance of the light source. In use, the mobile phone photographs the sample in the cuvette to extract color information, establishes a functional relationship between color information and sample concentration, and finally determines the concentration based on the color. This invention, combining a concentration gradient color chart and precise control of illumination, solves the problem of imaging differences between different mobile phones and promotes the widespread adoption of smartphone-based colorimetric detection methods.

[0103] This device's camera panel is made of magnetic material, allowing phones to adhere to it without the limitations of a SIM card slot. Different phones can be freely attached to the camera surface for taking pictures. The magnetic attachment ensures the phone stays firmly against the camera aperture, preventing light from entering from the side, while also freeing up your hands for more convenient and faster testing. The camera aperture on the panel has a diameter of 1 cm, larger than the size of a typical phone camera, making it compatible with various phones.

[0104] The light source and the dark box are designed separately and connected by magnetic attraction to facilitate the installation and replacement of faulty LED chips and internal control circuits.

[0105] The darkroom sample chamber is designed with three slots, which can hold three cuvettes at the same time, enabling simultaneous detection of three samples and improving detection speed.

[0106] The control circuit of the light source establishes Bluetooth communication with the mobile phone. The brightness of the light source is detected by the mobile phone and adjusted in real time to keep it consistent, ensuring the uniformity of illumination between different mobile phones and different measurements, and solving the problem of the influence of different illumination on the test results.

[0107] To create a concentration gradient color chart for different test solutions, users need to first photograph the color chart to form their own standard curve before testing the sample. This method can solve the problem of color differences when taking photos with different mobile phones.

[0108] This embodiment solves the problems of mobile phone placement, light source brightness control, and imaging differences between different mobile phones.

[0109] Example 3:

[0110] A multi-channel magnetic colorimetric detection device includes a light source, a dark chamber, a photographing device, and a concentration gradient color chart. The light source consists of an LED array and control unit, a diffuser, a switch, and a Type-C connector. The dark chamber includes a sample chamber and a photographing panel. The sample chamber has three slots and a cover plate. The photographing panel has a camera hole in the center. The photographing device includes a mobile phone and an app. When using the device, the user first takes a picture of the concentration gradient color chart with the mobile phone to establish a standard curve of color information versus concentration. Then, the cuvette containing the sample is placed in the slot, the cover plate is closed, and the mobile phone camera is positioned against the camera hole on the photographing panel to take a picture of the sample in the sample chamber. The mobile phone first uses a photosensor to determine if the light source brightness needs adjustment. After the brightness is adjusted, the mobile phone captures an image of the sample. The app then substitutes the color information from the image into the previously calculated standard curve to determine and display the sample concentration.

[0111] The light source and the dark box are separate, but they are connected by magnetic material, which facilitates the installation and maintenance of the light source.

[0112] The light emitted by the LED array in the light source is transformed into a uniform surface light source by a diffuser, which can be a semi-transparent material such as PMMA.

[0113] The LED array's brightness is adjusted by a control unit. This control unit has a Bluetooth transmitter module that establishes Bluetooth communication with a mobile phone. The phone's camera uses its own photosensor to identify the light source's brightness and sends commands to the control unit. The control unit then adjusts the LED's power supply voltage to change the light source's brightness. Adjustable brightness ensures consistent lighting conditions across different mobile phones and measurements, mitigating the impact of varying lighting conditions on test results. This method solves the problem of light source brightness control.

[0114] The Type-C connector connects to the USB power cable, serving as the power source for the light source.

[0115] The sample chamber is equipped with slots suitable for ordinary cuvettes. The slots ensure that the cuvettes placed inside do not shake. Three slots can hold three cuvettes at the same time, enabling simultaneous detection of multiple samples and improving the detection rate.

[0116] The camera panel is made of magnetic material, allowing phones to adhere to it without the restriction of a SIM card slot. Different phones can be freely attached to the camera surface for shooting. The magnetic attachment ensures the phone stays firmly against the camera hole, preventing light from entering from the side, while also freeing up your hands. This method is more convenient and faster during testing, solving the problem of phone placement.

[0117] The camera aperture on the camera panel is 1 cm in diameter, which is larger than the size of a typical mobile phone camera, making it suitable for taking photos with different mobile phones.

[0118] A concentration gradient color chart is a standard color chart created by taking images of test solutions of different concentrations. When using this device, users need to take a picture of the color chart to form their own standard curve before testing the sample. This method can solve the problem of color differences when taking pictures with different mobile phones.

[0119] The mobile app enables functions such as focusing on taking photos, controlling light source brightness, automatically capturing areas of interest, extracting color information, drawing calibration curves, and displaying reagent concentrations.

[0120] The color information mentioned above includes R (red), G (green), B (blue), H (hue), S (saturation), V (brightness), L (luminance), C (cyan), M (magenta), Y (yellow), K (black) and their algebraic combinations.

[0121] Example 4:

[0122] like Figure 1As shown, a multi-channel magnetic colorimetric detection device includes a light source 1, a dark chamber 2, a photographing device, and a concentration gradient color chart 3. The light source 1 consists of an LED array and control unit 4, a diffuser 5, a switch 6, and a Type-C connector 7. The dark chamber includes a sample chamber 8 and a photographing panel 9. The sample chamber 8 has three slots 10 and a cover 11. The photographing panel 9 has a camera hole 12 in the center. The photographing device includes a mobile phone and an app. In use, the mobile phone is first used to photograph the concentration gradient color chart 3 to establish a standard curve of color information and concentration. Then, a cuvette 13 containing the sample is placed in the slots 10, the cover 11 is closed, and the mobile phone camera is positioned against the camera hole 12 on the photographing panel 9 to photograph the sample in the sample chamber 8. The mobile phone first uses a photosensor to determine whether the brightness of the light source needs adjustment. After the brightness is adjusted, the mobile phone captures an image of the sample, and the app substitutes the color information of the image into the previous standard curve to calculate and display the sample concentration.

[0123] In this embodiment, the entire device is made of opaque black material with dimensions of 167*62*72mm. The light source 1 has dimensions of 42*62*72mm, the dark box 2 has dimensions of 125*62*72mm, and the cuvette 13 is a commercially available semi-micro 1.5 mL cuvette with dimensions of 12*12*45 mm.

[0124] In this embodiment, the LED array consists of flexible white-emitting patch LEDs bonded together in a 3x3 array, and the diffuser is made of PMMA material with a thickness of 1.5mm. The diffuser should not be too close to the LEDs, as this will result in uneven light spots. In this embodiment, the diffuser is 3cm away from the LEDs.

[0125] In this embodiment, the mobile phone establishes communication with the LED control unit via Bluetooth. Before each photo of the sample, the mobile phone first detects the brightness information of the light source. If the brightness information changes, it sends a command to the LED control unit to adjust the power supply voltage of the LED until the brightness information detected by the mobile phone reaches a predetermined value. The mobile phone is the core component of the colorimetric detection device, replacing the function of the spectrophotometer in traditional colorimetric detection. It takes a photo of the sample through the camera, processes the photo, and finally provides the concentration information of the sample. In this embodiment, the design flow of the developed mobile APP program is as follows: Figure 3 As shown, clicking the app icon on your phone's home screen will take you to the main interface. There are two function options: calibration and testing. Calibration involves photographing standard samples (or color charts) of different concentrations, extracting color information, and establishing a functional relationship between each color parameter and concentration. The testing interface is for testing samples of unknown concentrations. On this interface, you first need to select the optimal standard curve or manually input a standard curve; after selection, the concentration results will be automatically displayed.

[0126] To verify the effectiveness of this device, this embodiment is used for colorimetric detection of liquid formaldehyde based on gold nanoparticles. The principle of this colorimetric detection is that formaldehyde in the solution effectively triggers the reaction between the amino group of 4-ATP and the phenolic group of 4-MTP through the Mannich reaction, forming a compound with two thiol groups. The unreacted thiol groups react with the gold nanoparticles to form Au-S covalent bonds, thereby binding with the gold nanoparticles. The formation of a large number of Au-S covalent bonds induces the aggregation of gold nanoparticles, and the solution color changes from red to purple or even blue. Figure 4 A). The degree of aggregation of gold nanoparticles depends on the concentration of formaldehyde, so the concentration of formaldehyde can be determined by analyzing the color of the solution.

[0127] The standard curve obtained by measuring formaldehyde concentrations ranging from 0.1 to 1.0 μmol / L using this device is shown below. Figure 4 As shown in B, the corresponding G / B value decreases linearly with formaldehyde concentration within this range, and the linear equation is y = -0.038x + 0.927, RB 2 The value is 0.997. The standard curve obtained using a spectrophotometer is as follows: Figure 4 C, the absorbance ratio of gold nanoparticles, A600 / A520, increases with increasing formaldehyde concentration, and the linear equation is: y = 0.131x + 0.148, R 2 =0.973. Therefore, this device can replace a spectrophotometer for routine colorimetric detection, and it offers high accuracy.

[0128] To verify that the colorimetric chart method designed in this device can solve the problem of imaging differences between different mobile phones, this embodiment selects three different mobile phones—HUAWEI P30 Pro, Xiaomi 11 Pro, and MIX 2S—to detect the same concentration of formaldehyde (5 μmol / L). First, the developed APP was installed on the three mobile phones, and then... Figure 4 The formaldehyde concentration color chart in A was photographed, and a calibration formula adapted to each mobile phone was fitted. Then, the formaldehyde concentration to be tested was detected, and the actual test results are shown in Table 1.

[0129]

[0130] The deviation rate of the test results for the three different mobile phone models was around 5%, proving that this method can be used to solve the problem of different mobile phone photo effects.

Claims

1. A multi-channel magnetic colorimetric detection device, characterized in that: Includes a light source device (1), a dark box (2), a photographic device, a concentration gradient color chart (3), and one or more cuvettes (13); The light source device (1) provides illumination to the dark box (2); The dark box (2) includes a box body, a sample chamber (8), and a photographic panel (9); The sample chamber (8) is an open cavity at the top and is located inside the box; The sample chamber (8) is provided with one or more slots (10); the slots (10) are used to place cuvettes (13); A movable cover plate (11) is provided above the sample chamber (8); The photographing panel (9) is located on the side wall of the dark box (2) and faces the sample chamber (8). The camera panel (9) is provided with a camera hole (12); The photographing panel (9) is used to attach the photographing device; The cuvette (13) is used to hold the sample to be tested; The concentration gradient color card (3) is affixed to the inner wall of the sample chamber (8); The concentration gradient color chart (3) can be removed; The photographing device acquires images of the concentration gradient color card (3) and establishes a standard curve of color information and concentration; During colorimetric testing, the photographing device is magnetically attached to the photographing panel (9), and the camera of the photographing device is aligned with the camera hole (12). The photographing device captures images of the samples in the sample chamber (8), obtains color information of the sample images, and determines the sample concentration based on the standard curve of color information and concentration. The light source device (1) includes an LED array and a control unit (4). The control unit (4) controls the LED array to emit light; The light emitted by the LED array passes through the diffuser (5) and then illuminates the sample chamber (8); The camera device includes a mobile phone and a processing module mounted on the mobile phone; The mobile phone communicates with the control unit (4) via Bluetooth; The mobile phone detects the brightness of the LED array and transmits a control signal to the control unit (4) to control the brightness of the LED array.

2. The multi-channel magnetic colorimetric detection device according to claim 1, characterized in that: The multi-channel magnetic colorimetric detection device has two working states; The first working state is: the concentration gradient color card (3) is attached to the inner wall of the sample chamber (8); The second working state is: the concentration gradient color card (3) is not attached to the inner wall of the sample chamber (8), but the cuvette (13) contains the sample to be tested.

3. The multi-channel magnetic colorimetric detection device according to claim 1, characterized in that: The materials used in the diffuser (5) include translucent materials; The concentration gradient color chart (3) includes a semi-transparent material; The material of the dark box (2) includes opaque materials.

4. The multi-channel magnetic colorimetric detection device according to claim 1, characterized in that: The light source device (1) also includes a diffuser (5), a switch (6), and a Type-C connector (7); The switch (6) is used to control the operation of the light source device (1); An external power supply powers the LED array and control unit (4) via a Type-C connector (7).

5. The multi-channel magnetic colorimetric detection device according to claim 1, characterized in that: The mobile phone uses its built-in camera to capture images of the concentration gradient color chart (3) and the sample images in the sample chamber (8); The processing module establishes a standard curve of color information and concentration based on the image of the concentration gradient color chart (3); The processing module acquires the color information of the sample image and determines the sample concentration based on the standard curve of color information versus concentration.

6. The multi-channel magnetic colorimetric detection device according to claim 1, characterized in that: The light source device (1) and the dark box (2) are connected by magnetic material.

7. The multi-channel magnetic colorimetric detection device according to claim 1, characterized in that: The material of the camera panel (9) includes magnetic material.

8. The multi-channel magnetic colorimetric detection device according to claim 1, characterized in that: The concentration gradient color chart (3) is a color image of the sample to be tested at different standard concentrations. One concentration gradient color chart corresponds to one substance. The concentration gradient color chart (3) includes multiple color regions; different color regions have different color information; each color region is labeled with a corresponding sample concentration value; The color information includes red, green, blue, hue, saturation, brightness, lightness, cyan, magenta, yellow, black, and algebraic combinations of the above color information.

9. A detection method for a multi-channel magnetic colorimetric detection device, characterized in that, Includes the following steps: Step 1) Use a camera to capture images of the concentration gradient color chart (3) and establish a standard curve of color information versus concentration; Step 2) Place the sample to be tested for colorimetry into the cuvette (13); Open the movable cover (11) of the sample chamber (8), then put the cuvette (13) into the slot (10), close the movable cover (11) to seal the sample chamber (8); Step 3) Attach the camera device to the camera panel (9) and align the camera of the camera device with the camera hole (12). Step 4) Use the photographing device to capture the sample image in the sample chamber (8), obtain the color information of the sample image, and determine the sample concentration according to the standard curve of color information and concentration.

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