Artificial incubation device and method for fertilized eel eggs with water mold inhibition

Abstract

The invention discloses an artificial hatching device and method for fertilized eel eggs inhibiting water mold. The device comprises a water source treatment chamber, a water pipe is provided on the top of the water source treatment chamber, an ultraviolet disinfection lamp is provided in the water source treatment chamber, a water mold inhibitor feeding port is provided on the top of the water source treatment chamber, a water pipe is provided at the bottom of the water source treatment chamber, an atomizer is provided on the water pipe, an output end of the water pipe is connected to a mist collecting cover through a mist pipe, the mist collecting cover is provided on the top of a drain basket, the drain basket is provided on the top of a collection bucket, and fertilized eel eggs are placed at the bottom of the drain basket. The device can inhibit the occurrence of water mold disease, improve the hatching rate of fertilized eel eggs, realize the large-scale stable production of eel seedlings, and solve the problem of shortage of eel seedlings in breeding.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to an artificial hatching device and method for fertilized eel eggs capable of inhibiting water mold. Background Art

[0002] The yellow eel (Monopterus albus) is a specialty freshwater fish species that has seen rapid growth in aquaculture in my country in recent years. Its cultivation has spread throughout the country, making it a leading species in freshwater aquaculture in these regions. Currently, due to the lack of breakthroughs in large-scale propagation technology for yellow eel fry, large-scale artificial propagation is limited. The majority of released fry are still wild-caught, which cannot meet the fry demand of the growing yellow eel industry. Therefore, artificial mass production of yellow eel fry is urgently needed.

[0003] Currently, the main challenge facing the production of fertilized eel fry is the high incidence of Saprolegniasis during the incubation process, leading to widespread mortality. Existing technologies primarily employ artificial ecological incubation methods in rice field cages. This method relies on the protective function of the vesicles continuously expelled by parent eels during reproduction to reduce the risk of widespread mortality caused by Saprolegniasis during the incubation process. However, this method suffers from unstable production and low yields, failing to meet the growing demand for fry in the eel farming industry. Therefore, the development of an artificial incubation device and method for fertilized eel eggs that inhibits Saprolegniasis has become an urgent need. Summary of the Invention

[0004] The purpose of the present invention is to overcome the above-mentioned shortcomings and provide an artificial hatching device and method for fertilized eel eggs that inhibits water mold, so as to inhibit the occurrence of water mold disease, improve the hatching rate of fertilized eel eggs, realize the large-scale and stable production of eel seedlings, and solve the problem of shortage of eel breeding seedlings.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solution: an artificial hatching device for fertilized eels with the function of inhibiting water mold, comprising a water source treatment chamber, a water pipe is provided on the top of the water source treatment chamber, an ultraviolet disinfection lamp is provided in the water source treatment chamber, a water mold inhibitor feeding port is provided on the top of the water source treatment chamber, a water pipe is provided at the bottom of the water source treatment chamber, an atomizer is provided on the water pipe, the output end of the water pipe is connected to a mist collecting cover through a mist pipe, the mist collecting cover is provided on the top of a drain basket, the drain basket is provided on the top of a collection bucket, and fertilized eel eggs are placed at the bottom of the drain basket.

[0006] Preferably, a gate valve is provided on the water delivery pipe.

[0007] Preferably, a mist ball is provided at the output end of the water delivery pipe, and the mist ball is connected to a plurality of mist pipes.

[0008] Preferably, the mist collecting cover is a conical funnel structure, the drain basket is a truncated cone structure, the outer side wall of the bottom of the mist collecting cover contacts and cooperates with the inner side wall of the drain basket, and the outer side wall of the bottom of the drain basket contacts and cooperates with the inner side wall of the collection bucket.

[0009] Preferably, the top of the drain basket is open, and a plurality of sieve holes are evenly arranged on the bottom surface.

[0010] Preferably, the collection barrel is open at the top and provided with a water outlet pipe at the bottom.

[0011] In addition, the present invention also discloses a method for artificially hatching fertilized eel eggs to inhibit water mold, which comprises the following steps:

[0012] S1. Water source treatment: First, close the gate valve and use the water pipe to pass filtered tap water into the water source treatment chamber. Then start the ultraviolet disinfection lamp and irradiate the water source with ultraviolet light for a period of time and then turn it off to achieve the disinfection effect. Add water mold inhibitor to the water source treatment chamber through the feeding port;

[0013] S2. Hatching of fertilized eel eggs: Collect fertilized eel eggs that are naturally bred or artificially inseminated, spread them flat on the bottom of a drain basket, then place the drain basket on the top of a collection bucket, place a mist collecting cover on the top of the drain basket, open the gate valve and the atomizer, and allow the water source containing the water mold inhibitor to flow out through the water pipe at the bottom of the water source treatment chamber, and then flow to the mist collecting cover through the mist pipe after being atomized by the atomizer, and then evenly sprayed on the fertilized eel eggs at the bottom of the drain basket. Excess water enters the collection bucket through the sieve holes of the drain basket and is finally discharged from the outlet pipe;

[0014] S3. Treatment of dead individuals during the hatching process: Observe the survival of the fertilized eels in the drain basket and the fry in the collection bucket at regular intervals, and pick out the dead fertilized eels and fry;

[0015] S4. Collection of young eels: Pour out the hatched young eels in the collection bucket at regular intervals and place them in subsequent culture equipment until the fertilized eel eggs in the drain basket are completely hatched.

[0016] Furthermore, the preparation method of the Saprolegnia inhibitor in S1 is as follows:

[0017] S1.1: Prepare an adult yellow eel. After striking its head hard to stop it from struggling, secure its head with an awl. Use a scalpel to make a small incision in its throat. Slice backward to the tail, remove the head, and remove all internal organs and major blood vessels. Finally, wipe away any remaining blood from the eel's surface and body with absorbent paper.

[0018] S1.2: Cut the remaining dried eel into sections, place them in a container, add sterile water, and rub them. Repeat the rubbing for a period of time until the mucus on the eel surface is fully dissolved in the sterile water.

[0019] S1.3. Remove the eel segments, pour out and collect the solution in the container, add new sterile water to the container, and place the eel segments back into the container to soak for a period of time. After repeatedly rubbing and washing for a period of time, allow the mucus on the eel surface to fully dissolve in the sterile water.

[0020] S1.4 After repeating step S1.3 several times, the cut eels are fished out and the solution in the container is poured into a freeze dryer for freeze drying and concentration to obtain a water mold inhibitor.

[0021] Furthermore, the sterile water in steps S1.2 and S1.3 is low-temperature water.

[0022] Furthermore, the concentration of the Saprolegnia inhibitor obtained in S1.4 is 200 g / L.

[0023] Beneficial effects of the present invention:

[0024] 1. The present invention uses extracted eel mucus as a saprolegniasis inhibitor instead of previous antimicrobial peptide reagents, which reduces the incidence of saprolegniasis in eel fertilized eggs. Moreover, the saprolegniasis inhibitor treated with freeze-drying and concentration can perfectly maintain its antibacterial activity, overcomes the shortcomings of the artificial ecological breeding eel seedling hatching process, and improves the hatching rate of eel fertilized eggs.

[0025] 2. The ultraviolet disinfection lamp of the present invention can disinfect and sterilize the tap water introduced into the water source treatment room, and can provide good water quality conditions for the eels.

[0026] 3. The present invention utilizes a water mist spraying method to moisten the fertilized eel eggs, which can not only meet the water needs of the fertilized eel eggs, but also expose the fertilized eel eggs to the air and provide them with sufficient oxygen.

[0027] 4. The hatching method of the present invention is different from the previous method of placing the fertilized eel eggs directly in water. In the past, during hatching, the fertilized eel eggs and the eel seedlings after hatching in water coexisted in the same water area, which made it easier to cross-infect with water mold disease. The design of the present invention can separate the fertilized eel eggs and the eel seedlings from each other in two upper and lower areas, making cross infection less likely to occur.

[0028] 5. The present invention can inhibit the occurrence of Saprolegniasis by using the Saprolegniasis inhibitor, improve the hatching rate of fertilized eggs of the yellow eel, realize the large-scale stable production of yellow eel seedlings, and solve the problem of shortage of yellow eel breeding seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The schematic diagram of the structure of an artificial incubation device for fertilized eel eggs that inhibits water mold;

[0030] Figure 2 for Figure 1 Schematic diagram of the three-dimensional structure. DETAILED DESCRIPTION

[0031] The present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments.

[0032] like Figure 1 and 2 As shown, an artificial hatching device for fertilized eel eggs that inhibits water mold includes a water source treatment chamber 2, a water pipe 3 is provided on the top of the water source treatment chamber 2, an ultraviolet disinfection lamp 4 is provided in the water source treatment chamber 2, a water mold inhibitor feeding port 1 is provided on the top of the water source treatment chamber 2, a water pipe 6 is provided at the bottom of the water source treatment chamber 2, an atomizer 7 is provided on the water pipe 6, the output end of the water pipe 6 is connected to the mist collecting cover 10 through the mist pipe 9, the mist collecting cover 10 is provided on the top of the drain basket 11, the drain basket 11 is provided on the top of the collection bucket 12, and the fertilized eel eggs are placed at the bottom of the drain basket 11.

[0033] Preferably, a gate valve 5 is provided on the water delivery pipe 6. In this embodiment, the flow state of the water delivery pipe 6 can be controlled by opening and closing the gate valve 5.

[0034] Preferably, a capsule mist ball 8 is provided at the output end of the water delivery pipe 6, and the capsule mist ball 8 is connected to multiple mist pipes 9. In this embodiment, the capsule mist ball 8 is equivalent to a distributor, which evenly distributes the atomized solution to the corresponding mist pipes 9.

[0035] Preferably, the mist collecting cover 10 is a conical funnel structure, the drain basket 11 is a truncated cone structure, the bottom outer wall of the mist collecting cover 10 contacts and cooperates with the inner wall of the drain basket 11, and the bottom outer wall of the drain basket 11 contacts and cooperates with the inner wall of the collection bucket 12. Figure 1 As shown, since the mist collecting cover 10 is a conical funnel structure and the drain basket 11 is a truncated cone structure, the mist collecting cover 10 can be directly placed on the top of the drain basket 11, and the drain basket 11 can be placed on the top of the collection bucket 12, so that the entire equipment can be assembled. When it is necessary to take the hatched young eels in the collection bucket 12, the equipment can also be quickly separated. It is easy to disassemble and assemble, which greatly improves the hatching rate of fertilized eel eggs and realizes the large-scale and stable production of eel seedlings.

[0036] Preferably, the top of the drain basket 11 is open, and a plurality of sieve holes are evenly arranged on the bottom. In this embodiment, the particle size of the fertilized eel eggs is larger than the aperture of the sieve holes, so they can be placed on the bottom of the drain basket 11. When the fertilized eel eggs are hatched into eel seedlings, the diameter of the eel seedlings will be smaller than the aperture of the sieve holes, so they can enter the collection bucket 12 below through the sieve holes. This hatching method is different from the previous method of placing the fertilized eel eggs directly in water. In the past, when hatching, the eel seedlings and other unhatched eels after the fertilized eel eggs are hatched in water are separated. Fertilized eggs coexist in the same water area, which makes it easier for them to be cross-infected with water mold disease. The design of the present invention can separate the fertilized eel eggs and the eel seedlings from each other in two upper and lower areas. The fertilized eel eggs located at the upper area are not immersed in water, but the present invention uses a water mist spraying method to moisten the fertilized eel eggs, which can not only meet the water needs of the fertilized eel eggs, but also expose the fertilized eel eggs to the air, with sufficient oxygen, thereby greatly improving the hatching rate.

[0037] Preferably, the collection barrel 12 has an open top and a water outlet pipe 13 at its bottom.

[0038] In addition, the present invention also discloses a method for artificially hatching fertilized eel eggs to inhibit water mold, which comprises the following steps:

[0039] S1. Water source treatment: First, close the gate valve 5 and use the water pipe 3 to pass filtered tap water into the water source treatment chamber 2. Then, start the ultraviolet disinfection lamp 4, irradiate the water source with ultraviolet light for a period of time, and then turn it off to achieve the disinfection effect; add water mold inhibitor to the water source treatment chamber 2 through the feeding port 1;

[0040] S2, hatching of fertilized eel eggs: collect fertilized eel eggs that are naturally bred or artificially inseminated, spread them on the bottom of the drain basket 11, then put the drain basket 11 on the top of the collection bucket 12, put the mist collecting cover 10 on the top of the drain basket 11, open the gate valve 5 and the atomizer 7, let the water containing the water mold inhibitor flow out through the water pipe 6 at the bottom of the water source treatment chamber 2, and then flow to the mist collecting cover 10 through the mist pipe 9 after being atomized by the atomizer 7, and then evenly sprayed on the fertilized eel eggs at the bottom of the drain basket 11, and the excess water enters the collection bucket 12 through the sieve holes of the drain basket 11, and is finally discharged from the outlet pipe 13;

[0041] S3. Processing of dead individuals during the hatching process: Observe the survival of the fertilized eggs of the swamp eel in the drain basket 11 and the juvenile eels in the collection bucket 12 at regular intervals, and pick out the dead fertilized eggs of the swamp eel and the juvenile eels;

[0042] S4. Collection of young eels: pour out the hatched young eels in the collection bucket 12 at regular intervals and place them in subsequent culture equipment until the fertilized eel eggs in the drain basket 11 are completely hatched.

[0043] Furthermore, the preparation method of the Saprolegnia inhibitor in S1 is as follows:

[0044] S1.1: Prepare an adult yellow eel. After striking its head hard to stop it from struggling, secure its head with an awl. Use a scalpel to make a small incision in its throat. Slice backward to the tail, remove the head, and remove all internal organs and major blood vessels. Finally, wipe away any remaining blood from the eel's surface and body with absorbent paper.

[0045] S1.2: Cut the remaining dried eel into sections, place them in a container, add sterile water, and rub them. Repeat the rubbing for a period of time until the mucus on the eel surface is fully dissolved in the sterile water.

[0046] S1.3. Remove the eel segments, pour out and collect the solution in the container, add new sterile water to the container, and place the eel segments back into the container to soak for a period of time. After repeatedly rubbing and washing for a period of time, allow the mucus on the eel surface to fully dissolve in the sterile water.

[0047] S1.4 After repeating step S1.3 multiple times, remove the segmented eels and pour the solution in the container into a freeze dryer for freeze-drying and concentration to obtain a Saprolegnia inhibitor. The eel mucus extracted in this example is an antibacterial glycoprotein that can be used as a Saprolegnia inhibitor, replacing conventional antimicrobial peptide reagents, to reduce the incidence of disease in eel fertilized eggs.

[0048] Furthermore, the sterile water in steps S1.2 and S1.3 is low-temperature water. In this embodiment, the low-temperature water is 4°C sterile water.

[0049] Furthermore, the concentration of the Saprolegnia inhibitor obtained in S1.4 is 200 g / L.

[0050] Example 1:

[0051] ① Water source treatment: First, close the gate valve and use the water pipe to introduce filtered tap water into the water source treatment chamber to fill 9 / 10 of the entire water source treatment chamber. Start the ultraviolet disinfection lamp and irradiate the water source with ultraviolet light for 30 seconds and then turn it off to achieve the disinfection effect; add 500mL of 200g / L of the above-mentioned water mold inhibitor into the water source treatment chamber through the feed port and mix well.

[0052] ② Hatching of fertilized eel eggs: Collect fertilized eel eggs, either naturally bred or artificially inseminated, and spread them flat on the bottom of a drain basket with a 2mm pore size (the drain basket is 12cm in diameter). Place approximately 1,000 fertilized eel eggs in each basin, for a total of 3,000 eggs in three basins. Open the gate valve and atomizer, allowing water containing saprolegniasis inhibitors to flow through the water pipe at the bottom of the water treatment chamber. After being atomized by the atomizer, the water flows out through the mist pipe and is evenly sprayed on the fertilized eel eggs. Excess water is discharged through a collection bucket and outlet pipe.

[0053] ③Handling of dead individuals during the hatching process: Observe the survival of the fertilized eel eggs in the drain basket and the eel fry in the collection bucket every 6 hours. The dead fertilized eel eggs and eel fry need to be picked out manually using tools such as droppers.

[0054] ④ Collection of young eels: Every three days, hatched young eels were removed from the collection bucket and placed in subsequent culture equipment until all fertilized eggs in the drain baskets were hatched. A total of 2,745 young eels were hatched. See Table 1 below for details:

[0055] Table 1 Example 1 Eel fertilized egg hatching results

[0056] Number of fish eggs Number of fry hatching rate 3000 2745 91.5％

[0057] In this embodiment, the water source treatment chamber in the device is 1m long, 0.5m wide and 0.5m high; a water pipe with a diameter of 10cm is connected to the right side of the water source treatment chamber, and an ultraviolet disinfection lamp with a length of 0.5m is installed inside. There is a feeding port with a diameter of 5cm on the upper side, and a water pipe with a diameter of 5cm is installed on the lower side to be connected to the atomizer, with a gate valve in the middle; the atomizer is 20cm long, wide and high, and 20 mist pipes with a diameter of 2cm are connected to the drain basket on the lower side; a mist collecting cover with a lower diameter of 14cm is installed at the end of the mist pipe; the upper diameter of the drain basket is 16cm and the lower diameter is 12cm. It is placed on a collection bucket with a diameter of 14cm and a height of 40cm. The collection bucket is connected to a drain pipe with a diameter of 2cm at 2cm from the bottom.

[0058] The specific method for preparing the Saprolegnia inhibitor in Example 1 is as follows:

[0059] S1.1: Prepare an adult yellow eel. After striking its head hard to stop it from struggling, secure its head with an awl. Use a scalpel to make a small incision in its throat. Slice backward to the tail, remove the head, and remove all internal organs and major blood vessels. Finally, wipe away any remaining blood from the eel's surface and body with absorbent paper.

[0060] S1.2: Cut the remaining eel into approximately 4 cm segments, place them in a container, add 500 mL of 4°C sterile water, and rub them. Repeat this process for 2 minutes to allow the mucus on the eel's surface to fully dissolve in the sterile water.

[0061] S1.3. Remove the eel segments, pour out and collect the solution in the container, add fresh 4°C sterile water to the container, and place the eel segments back in the container to soak for 3 minutes. Knead and wash the eel again for 2 minutes to allow the mucus on the eel surface to fully dissolve in the sterile water.

[0062] S1.4 After repeating step S1.3 4-5 times, remove the segmented eels and pour the solution in the container into a freeze dryer for freeze-drying and concentration to obtain 200 g / L of saprolegnia inhibitor.

[0063] Example 2 (traditional ecological breeding eel hatching process):

[0064] ① Water source treatment: First close the gate valve, use the water pipe to introduce filtered tap water into the water source treatment room, so that it fills 9 / 10 of the entire water source treatment room, start the ultraviolet disinfection lamp, irradiate the water source with ultraviolet light for 30 seconds and then turn it off to achieve the disinfection effect.

[0065] ② Hatching of Fertilized Eel Eggs: Collect fertilized eel eggs, either naturally bred or artificially inseminated, and spread them flat on the bottom of a drain basket with a 2mm pore size (12cm in diameter). Place 1,000 fertilized eel eggs per basin. For a total of 3,000 eggs across 3 basins, turn on the atomizer and allow water to flow through the water pipe at the bottom of the water treatment chamber. After being atomized by the atomizer, the water flows out through the mist pipe and is evenly sprayed on the fertilized eel eggs. Excess water is discharged through a collection bucket and drain pipe.

[0066] ③Handling of dead individuals during the hatching process: Observe the survival of the fertilized eel eggs in the drain basket and the eel fry in the collection bucket every 6 hours. The dead fertilized eel eggs and eel fry need to be picked out manually using tools such as droppers.

[0067] ④ Collection of juvenile eels: Every three days, hatched juvenile eels were removed from the collection bucket and placed in subsequent culture equipment until all fertilized eggs in the drain baskets had hatched. A total of 1,349 juvenile eels were hatched.

[0068] Table 2 Example 2 Eel fertilized egg hatching results

[0069] Number of fish eggs Number of fry hatching rate 3000 1349 45.0％

[0070] The sizes of the various parts of the device in this embodiment 2 are the same as those in embodiment 1.

[0071] Result analysis:

[0072] From the above comparison, it can be seen that Example 1 uses a water mold inhibitor to treat the water source of the fertilized eel eggs, and supplemented by ultraviolet lamp disinfection method, so that the hatching rate of the fertilized eel eggs is increased from about 45% in Example 2 to more than 90%, overcoming the shortcomings of the traditional ecological breeding eel seedling hatching process and solving the current demand for eel seedlings in eel farming.

[0073] The above embodiments are merely preferred technical solutions of the present invention and should not be construed as limiting the present invention. The embodiments and features in the embodiments of this application may be arbitrarily combined with each other unless they conflict. The scope of protection of the present invention shall be the technical solutions described in the claims, including equivalent alternatives to the technical features of the technical solutions described in the claims. Equivalent alternatives and improvements within this scope are also within the scope of protection of the present invention.

Claims

1. An artificial incubation device for fertilized eel eggs that inhibits water mold, comprising a water source treatment chamber (2), a water pipe (3) provided on the top of the water source treatment chamber (2), and an ultraviolet disinfection lamp (4) provided inside the water source treatment chamber (2), characterized in that: The water source treatment chamber (2) is provided with a saprolegniasis inhibitor feeding port (1) at the top, a water delivery pipe (6) is provided at the bottom of the water source treatment chamber (2), an atomizer (7) is provided on the water delivery pipe (6), an output end of the water delivery pipe (6) is connected to a mist collecting cover (10) through a mist pipe (9), the mist collecting cover (10) is provided on the top of a drain basket (11), the drain basket (11) is provided on the top of a collecting bucket (12), and fertilized eel eggs are placed at the bottom of the drain basket (11); the mist collecting The cover (10) is a conical funnel structure, the drain basket (11) is a truncated cone structure, the outer side wall of the bottom of the mist collecting cover (10) contacts and cooperates with the inner side wall of the drain basket (11), and the outer side wall of the bottom of the drain basket (11) contacts and cooperates with the inner side wall of the collecting bucket (12); the top of the drain basket (11) is open, and a plurality of sieve holes are evenly opened on the bottom surface; the aperture of the sieve holes is larger than the diameter of the eel seedling and smaller than the particle size of the eel fertilized egg; the water mold inhibitor is mucus on the surface of the eel.

2. The artificial incubation device for fertilized eel eggs for inhibiting water mold according to claim 1, wherein: A gate valve (5) is provided on the water delivery pipe (6).

3. The artificial incubation device for fertilized eel eggs for inhibiting water mold according to claim 1, characterized in that: A mist ball (8) is provided at the output end of the water delivery pipe (6), and the mist ball (8) is connected to a plurality of mist pipes (9).

4. The artificial incubation device for fertilized eel eggs for inhibiting water mold according to claim 1, characterized in that: The collecting barrel (12) is open at the top and provided with a water outlet pipe (13) at the bottom.

5. A method for hatching fertilized eel eggs artificially hatched with the Saprolegnia albus device according to claim 4, characterized in that: It includes the following steps: S1. Water source treatment: First, close the gate valve (5), and use the water pipe (3) to pass filtered tap water into the water source treatment chamber (2). Then, start the ultraviolet disinfection lamp (4), irradiate the water source with ultraviolet light for a period of time, and then turn it off to achieve the disinfection effect; add water mold inhibitor to the water source treatment chamber (2) through the feeding port (1); S2. Hatching of fertilized eel eggs: Collect fertilized eel eggs that are naturally bred or artificially fertilized, and spread them flat on the bottom of the drain basket (11), then place the drain basket (11) on the top of the collection bucket (12), place the mist collecting cover (10) on the top of the drain basket (11), open the gate valve (5) and the atomizer (7), and let the water source containing the water mold inhibitor flow out through the water pipe (6) at the bottom of the water source treatment chamber (2), and then flow from the mist pipe (9) to the mist collecting cover (10) after being atomized by the atomizer (7), and then evenly sprayed on the fertilized eel eggs at the bottom of the drain basket (11). The excess water source enters the collection bucket (12) through the sieve holes of the drain basket (11) and is finally discharged from the outlet pipe (13); S3. Treatment of dead individuals during the hatching process: observe the survival of the fertilized eel eggs in the drain basket (11) and the eel fry in the collection bucket (12) at regular intervals, and pick out the dead fertilized eel eggs and eel fry; S4. Collection of young eels: Pour out the hatched young eels in the collection bucket (12) at regular intervals and place them in subsequent culture equipment until the fertilized eel eggs in the drain basket (11) are completely hatched.

6. The method for hatching the fertilized eel eggs artificially hatched by the water mold inhibition device according to claim 5, characterized in that: The preparation method of the Saprolegnia inhibitor in S1 is as follows: S1.1: Prepare an adult yellow eel. After striking its head hard to stop it from struggling, secure its head with an awl. Use a scalpel to make a small incision in its throat. Slice backward to the tail, remove the head, and remove all internal organs and major blood vessels. Finally, wipe away any remaining blood from the eel's surface and body with absorbent paper. S1.2: Cut the remaining dried eel into sections, place them in a container, add sterile water, and rub them. Repeat the rubbing for a period of time until the mucus on the eel surface is fully dissolved in the sterile water. S1.

3. Remove the eel segments, pour out and collect the solution in the container, add new sterile water to the container, and place the eel segments back into the container to soak for a period of time. After repeatedly rubbing and washing for a period of time, allow the mucus on the eel surface to fully dissolve in the sterile water. S1.4 After repeating step S1.3 several times, the cut eels are fished out and the solution in the container is poured into a freeze dryer for freeze drying and concentration to obtain a water mold inhibitor.

7. The hatching method of the artificial hatching equipment for fertilized eel eggs that inhibits water mold according to claim 6, wherein: The sterile water in steps S1.2 and S1.3 is low-temperature water.

8. The hatching method of the artificial hatching equipment for fertilized eel eggs that inhibits water mold according to claim 6, characterized in that: The concentration of the Saprolegnia inhibitor obtained in S1.4 is 200 g / L.

Images