A low-temperature disinfectant and its preparation method and application

Abstract

The present invention belongs to the technical field of low-temperature disinfectants, and specifically provides a low-temperature disinfectant and a preparation method and application thereof. The low-temperature disinfectant provided in this application includes calcium chloride, sodium hypochlorite, sodium benzoate, hydroxybenzotriazole and water. The low-temperature disinfectant is in a liquid state at ‑20°C to ‑40°C, and has a good killing effect on the new coronavirus, adenovirus, and HSV‑2. When using a low-temperature disinfectant on a refrigerator or cold storage, the low-temperature disinfectant provided by the present invention can protect the refrigerator, cold storage or other low-temperature devices from corrosion. The low-temperature disinfectant provided by the present invention is suitable for disinfection of low-temperature environments of ‑20°C to ‑40°C and the surface of the outer packaging of articles.

Description

Technical Field

[0001] The present invention relates to the technical field of low-temperature disinfectants, and in particular to a low-temperature disinfectant and a preparation method and application thereof. Background Art

[0002] Commonly used disinfectants on the market, such as 84 disinfectant (sodium hypochlorite), are used at room temperature. Conventional disinfectants cannot disinfect ice in low-temperature environments such as cold storage. In addition, conventional disinfectants will turn from liquid to solid in winter when the temperature is below 0°C, making it difficult to disinfect and the disinfection ability will decrease.

[0003] The existing 84 disinfectant is a chlorine-containing disinfectant, the main ingredient of which is sodium hypochlorite. It is usually used at room temperature. In addition, the current chlorine-containing disinfectant is corrosive and can easily corrode metal equipment, affecting the life of the machine and possibly causing the machine to be shut down.

[0004] Cold chain transportation or cold storage includes: using standard refrigerated transport vehicles to transport frozen foods, meat, ice cream and other goods; or using standard cold storage to store goods at -18℃ to -22℃.

[0005] In cold seasons and cold storage environments, the development of low-temperature disinfectants is crucial. According to the disinfection needs of the cold chain market, there is an urgent need for a low-temperature disinfectant that remains liquid and active in a sub-zero environment, which can meet the disinfection needs of cold storage and product packaging in the production, processing, storage, transportation, circulation, and trading of cold chain, as well as the needs of household cold freshness and winter disinfection. Summary of the invention

[0006] The present invention provides a low-temperature disinfectant capable of sterilizing at -20°C or below, and a preparation method and application thereof.

[0007] In a first aspect, the present invention provides a low-temperature disinfectant comprising calcium chloride, sodium hypochlorite, sodium benzoate, hydroxybenzotriazole and water.

[0008] In the low-temperature disinfectant formula of the present application, sodium hypochlorite is used as a bactericidal component, calcium chloride is used as an antifreeze substance, and sodium benzoate and hydroxybenzotriazole are used as preservatives.

[0009] In the prior art, chlorine-containing disinfectants can play a disinfecting role at room temperature. However, when the temperature is -20°C or below, the chlorine-containing disinfectant will solidify and cannot play a disinfecting role.

[0010] In the prior art, antifreeze is added to chlorine-containing disinfectants to maintain the liquid state of the disinfectants. However, inappropriate antifreeze will reduce the sterilization and disinfection effect of chlorine-containing disinfectants.

[0011] The present invention adopts calcium chloride and sodium hypochlorite in a specific ratio and combines them with a specific preservative to obtain a low-temperature disinfectant that can achieve good sterilization and disinfection effects at -20°C to -40°C.

[0012] In the low-temperature disinfectant provided by the present invention, the usage ratio of calcium chloride to sodium hypochlorite is: (150-200): (0.75-2).

[0013] In the low-temperature disinfectant provided by the present invention, the ratio of sodium hypochlorite to sodium benzoate and hydroxybenzotriazole in the low-temperature disinfectant is: (0.75-2): (0.5-1.5): (0.3-0.8) by mass ratio.

[0014] In the low-temperature disinfectant provided by the present invention, the ratio of calcium chloride, sodium hypochlorite, sodium benzoate, hydroxybenzotriazole and water in the low-temperature disinfectant is (150-200): (0.75-2): (0.5-1.5): (0.3-0.8): 1000 by mass ratio.

[0015] The preparation method of the low-temperature disinfectant provided by the present invention comprises: firstly mixing calcium chloride and sodium hypochlorite uniformly in water, then adding sodium benzoate and hydroxybenzotriazole into the uniformly mixed liquid, and further mixing uniformly.

[0016] In a second aspect, the present invention also provides a method for disinfecting an environment or an object at a temperature between 0°C and -40°C, wherein the above-mentioned low-temperature disinfectant is sprayed on the surface of the environment or the object.

[0017] In the disinfection method provided by the present invention, the ratio of the amount of the low-temperature disinfectant to the area of ​​the environment or the article is: 1L: (5-15m 2 ).

[0018] The beneficial effects of this application are:

[0019] (1) The low-temperature disinfectant obtained in the present application is in a liquid state at -20°C to -40°C, has a good killing effect on viruses, especially the new coronavirus (SARS-CoV-2), and has no toxic effect on cells.

[0020] (2) The present invention has experimentally confirmed the killing effect of low-temperature disinfectant on the new coronavirus, adenovirus, and HSV-2 under the conditions of -20°C and -40°C in the refrigerator, and confirmed that the low-temperature disinfectant provided by the present application can have a good killing effect on the new coronavirus, adenovirus, and HSV-2 under the conditions of -20°C and -40°C.

[0021] (3) The present invention can protect refrigerators or other low-temperature devices from corrosion by adding preservatives when low-temperature disinfectants are used in refrigerators and cold storages. It is suitable for disinfection of low-temperature environments of -40°C or above and the surface of outer packaging of items. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example 1

[0024] The present embodiment provides a low-temperature disinfectant, the formula and preparation method of which are as follows: prepare a calcium chloride concentration of 175 g / L and a sodium hypochlorite concentration of 2 g / L, first mix the calcium chloride and sodium hypochlorite evenly, then add 1 g of sodium benzoate and 0.5 g of hydroxybenzotriazole to the evenly mixed liquid, and further mix until evenly mixed.

[0025] Example 2

[0026] The present embodiment provides a low-temperature disinfectant, the formula and preparation method of which are as follows: prepare a calcium chloride concentration of 150 g / L and a sodium hypochlorite concentration of 2 g / L, first mix the calcium chloride and sodium hypochlorite evenly, then add 0.5 g of sodium benzoate and 0.3 g of hydroxybenzotriazole to the evenly mixed liquid, and further mix until evenly mixed.

[0027] Example 3

[0028] The present embodiment provides a low-temperature disinfectant, the formula and preparation method of which are as follows: prepare a calcium chloride concentration of 200 g / L and a sodium hypochlorite concentration of 2 g / L, first mix the calcium chloride and sodium hypochlorite evenly, then add 1.5 g of sodium benzoate and 0.8 g of hydroxybenzotriazole to the evenly mixed liquid, and further mix evenly.

[0029] Experimental Example 1 Optimal Temperature Detection of Low-Temperature Disinfectant

[0030] This experimental example tested the optimal disinfection temperature of the low-temperature disinfectants obtained in Examples 1-3. Specifically, in this experimental example, the coagulation degree of each disinfectant at different temperatures and the sterilization and disinfection effect of the disinfectant were specifically tested, see Table 1 and Table 2.

[0031] Table 1 Coagulation degree of low temperature disinfectant of Examples 1-3

[0032] Degree of solidification Example 1 Example 2 Example 3 normal temperature No solidification No solidification No solidification -4℃ No solidification No solidification No solidification -20℃ No solidification No solidification No solidification -40℃ No solidification No solidification No solidification -60℃ solidification solidification solidification -80℃ solidification solidification solidification

[0033] Table 2 Sterilization effect of low temperature disinfectant of Examples 1-3

[0034] Sterilization Example 1 Example 2 Example 3 normal temperature efficient efficient efficient -4℃ efficient efficient efficient -20℃ efficient efficient efficient -40℃ efficient efficient efficient -60℃ invalid invalid invalid -80℃ invalid invalid invalid

[0035] The above results show that the disinfectant obtained in the embodiment of the present invention also has the effect of inactivating viruses at room temperature and -4°C.

[0036] Experimental Example 2: Killing effect of low temperature disinfectant on different viruses

[0037] This experimental example evaluates the killing effect of the antiseptic low-temperature disinfectant obtained in Example 1 on different viruses at -20°C and -40°C. Three viruses were selected for the experiment, namely, the new coronavirus (SARS-CoV-2), adenovirus (ADV), and herpes simplex virus type 2 (HSV-2). The sensitive cells used for the above viruses were Vero, Hep-2, and Vero cells. The results are shown in Tables 3, 4, 5, 6, 7, and 8. In the tables, "+" represents lesions and "-" represents normal.

[0038] Table 3 The killing effect of low-temperature disinfectant on the new coronavirus at -20℃ in the refrigerator

[0039]

[0040] Table 4 The killing effect of low-temperature disinfectant on the new coronavirus in a refrigerator at -40℃

[0041]

[0042]

[0043] Table 5 The killing effect of low-temperature disinfectant on adenovirus in a refrigerator at -20℃

[0044]

[0045] Table 6 The killing effect of low-temperature disinfectant on adenovirus in a refrigerator at -40°C

[0046]

[0047] Table 7 The killing effect of low-temperature disinfectant on HSV-2 in a refrigerator at -20°C

[0048]

[0049]

[0050] Table 8 The killing effect of low-temperature disinfectant on HSV-2 in a refrigerator at -40°C

[0051]

[0052] Comparative Example 1 Different ratios of calcium chloride and sodium hypochlorite

[0053] The low-temperature disinfectant provided in this comparative example is different from that in Example 2 in that the ratio of calcium chloride to sodium hypochlorite in the low-temperature disinfectants A and C in this comparative example is 100:2 or 150:2 (the same as in Example 2).

[0054] The same method as in Experimental Example 2 was used to test the killing effects of three low-temperature disinfectants on the new coronavirus at -20°C in a refrigerator, see Table 9.

[0055] Table 9 The killing effect of low-temperature disinfectant on the new coronavirus in a refrigerator at -20℃

[0056]

[0057] In the table, + represents cell pathology; - represents normal cell growth

[0058] The results of this comparative example confirm that calcium chloride and sodium hypochlorite can only play a synergistic effect in an appropriate ratio.

[0059] Comparative Example 2 Different antifreeze

[0060] This comparative example provides a low-temperature disinfectant. The difference between the low-temperature disinfectant D in this comparative example and Example 2 is that in this comparative example, calcium chloride + magnesium chloride are used as antifreeze agents.

[0061] The low-temperature disinfectant obtained in Example 2 was taken as C, and the same method as in Experimental Example 2 was used to test the killing effects of the three low-temperature disinfectants on the new coronavirus under the condition of -20°C in the refrigerator, as shown in Table 10.

[0062] Table 10 The killing effect of low-temperature disinfectant on the new coronavirus at -20℃ in the refrigerator

[0063]

[0064] The results of this comparative example confirm that only by using a suitable antifreeze agent can a synergistic effect be achieved with calcium chloride and sodium hypochlorite.

[0065] Comparative Example 3 Different preservatives

[0066] This comparative example provides a low-temperature disinfectant. The difference between the low-temperature disinfectants F and G in this comparative example and those in Example 2 is that in this comparative example, propyl parahydroxybenzoate and potassium sorbate are used as preservatives.

[0067] The low-temperature disinfectant obtained in Example 2 was taken as C. The same method as in Experimental Example 2 was used to test the killing effects of the three low-temperature disinfectants on the new coronavirus under the condition of -20°C in the refrigerator, as shown in Table 11.

[0068] Table 11 The killing effect of low-temperature disinfectant on the new coronavirus in a refrigerator at -20℃

[0069]

[0070] The results of this comparative example confirm that although propyl paraben or potassium sorbate can replace sodium benzoate and hydroxybenzotriazole, good results can also be achieved; however, considering that the physical and chemical properties of sodium benzoate and hydroxybenzotriazole are relatively stable in liquid environments and are not easy to react with other substances, only by using appropriate preservatives can they play a stable synergistic role with calcium chloride and sodium hypochlorite.

[0071] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A low-temperature disinfectant, characterized in that: It includes calcium chloride, sodium hypochlorite, sodium benzoate, hydroxybenzotriazole and water; in terms of mass, the ratio of sodium hypochlorite to sodium benzoate and hydroxybenzotriazole in the low-temperature disinfectant is: (0.75-2): (0.5-1.5): (0.3-0.8); In terms of mass, the ratio of calcium chloride to sodium hypochlorite in the low-temperature disinfectant is: (150-200): (0.75-2).

2. The low-temperature disinfectant according to claim 1, characterized in that: In terms of mass, the ratio of calcium chloride, sodium hypochlorite, sodium benzoate, hydroxybenzotriazole and water in the low-temperature disinfectant is (150-200): (0.75-2): (0.5-1.5): (0.3-0.8): 1000.

3. The method for preparing the low-temperature disinfectant according to claim 1 or 2, characterized in that: include: First, calcium chloride and sodium hypochlorite are uniformly mixed in water, and then sodium benzoate and hydroxybenzotriazole are added to the uniformly mixed liquid, and the mixture is further uniformly mixed.

4. A method for disinfecting an environment or article below -20°C, characterized in that: Spray the low-temperature disinfectant according to any one of claims 1 to 3 onto the environment or the surface of an object.

5. The disinfection method according to claim 4, characterized in that: The ratio of the amount of the low-temperature disinfectant to the area of ​​the environment or the item is: 1L: (5-15m 2 ).

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