Natural active compound with moisturizing and skin repairing effects and its application

Abstract

The present invention relates to the field of cosmetics, and discloses a natural active compound with moisturizing and skin repairing effects and its application. The present invention is the first to compound Balkan chayote leaf extract with ectoine to obtain a natural active compound and apply it to cosmetics, and it is found that Balkan chayote leaf extract and ectoine can have a synergistic effect, which can significantly improve the moisturizing effect and skin repairing effect.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to a natural active compound with moisturizing and skin repairing effects and application thereof. Background Art

[0002] On the one hand, in modern society, people generally face great work pressure or study pressure. When people are under great pressure for a long time, their skin will age faster. On the other hand, people's awareness of beauty in modern society is gradually increasing. Therefore, how to delay skin aging has become one of the research hotspots in the current skin care field. Human skin aging is usually manifested as rough, dull, loose skin, etc. Skin aging can be divided into endogenous aging and exogenous aging according to influencing factors. Among them, endogenous aging is mainly due to the natural increase of age, low mitochondrial function, reduced DNA methylation, shortened telomeres, and decreased ability to synthesize collagen, elastin and extracellular matrix. Exogenous aging is closely related to external factors such as mentality, lifestyle and environmental pollution.

[0003] There are a wide variety of skin care products on the market, many of which contain natural active ingredients from plants, animals or microorganisms. These different types of natural active ingredients have different functions such as moisturizing, anti-oxidation, and anti-wrinkle.

[0004] Balkan lettuce belongs to the Gesneriaceae family and comes from the Rhodope Mountains in Europe (Bulgaria and Greece). Balkan lettuce is able to downregulate its metabolism in severe drought conditions. After exposure to rain again, it will be reactivated, so it is also called the "resurrection grass". In biological studies on Balkan lettuce, Balkan lettuce leaf extract reactivated the expression of key genes and protein production. Using this biological activity, studies have found that applying it to the field of skin care can effectively delay skin aging and repair the skin. For example, patent CN202111508687.6 discloses an anti-wrinkle skin care product, the raw materials of which include: 1-5% hydroxypropyl tetrahydropyrantriol, 0.1-1.5% adenosine, 0.005-0.03% cyanocobalamin, 2-10% collagen peptide, 0.05-0.2% retinol retinoate, 0.1-1% amino acid zinc, 1-2% Balkan lettuce leaf extract, 0.01-0.05% hydroxydecyl ubiquinone, 0.1-2% solubilizer, and 78-90% other excipients. The document reports that Balkan lettuce leaf extract is compounded with retinol retinoate, amino acid zinc, Balkan lettuce leaf extract and hydroxydecyl ubiquinone to achieve an anti-wrinkle effect.

[0005] Ecdoin is a cyclic amino acid derivative extracted from non-halophilic bacteria such as halophilic bacteria and streptococci that can grow in high temperature, high salt and high ultraviolet light environments. Existing literature has reported that Ecdoin has the effects of delaying skin aging, UV protection, anti-oxidation, moisturizing, etc. For example, patent CN202211150330.X discloses a soothing and repairing composition after sun exposure, including 0.5 to 3 parts by weight of DNA sodium; 0.4 to 1.5 parts by weight of plankton extract; 0.1 to 0.5 parts by weight of resveratrol; 1 to 20 parts by weight of Ecdoin. In this invention, Ecdoin plays a role in sun protection and water retention for the skin. The composition obtained by combining Ecdoin with other components can not only soothe the skin and calm the skin, but also effectively hydrate the skin, effectively improve the symptoms of redness, stinging, itching, etc. after sun exposure, and can also promptly remove free radicals generated by ultraviolet radiation, inhibit the production of melanin, and help the skin recover health. Wang Yuling et al. also reported in a paper (Study on the anti-photoaging effect of a composition based on hyaluronic acid and ectoine, Daily Chemicals Science) that the anti-photoaging composition obtained by compounding ectoine with hyaluronic acid and ergothioneine is non-toxic to fibroblasts within a concentration range of 5% and can be used safely; at a concentration of 2%, the product has an inhibition rate of 17.55% on reactive oxygen species induced by blue light radiation, and has a defensive and antagonistic effect on reactive oxygen damage caused by blue light irradiation. After the damage occurs, it can inhibit the expression of MMP-1 with an inhibition rate of 36.33%, and significantly increase the secretion of Collagen I (the increase rate is 113.02%), thereby improving the photoaging problem caused by blue light damage.

[0006] In summary, although there are related studies in the prior art on the respective effects of Balkan lettuce leaf extract and ectoine or the combination of one of the two with other active ingredients, to date, no relevant reports have been found on the efficacy research of the combination of Balkan lettuce leaf extract and ectoine. Summary of the invention

[0007] In order to solve the above technical problems, the present invention provides a natural active compound with moisturizing and skin repairing effects and its application. The present invention firstly compounded Balkan chicory leaf extract with ectoine to obtain a natural active compound and applied it to cosmetics. It was found that Balkan chicory leaf extract and ectoine can have a synergistic effect, which can significantly improve the moisturizing effect and skin repairing effect.

[0008] The specific technical scheme of the present invention is:

[0009] In a first aspect, the present invention provides a natural active compound with moisturizing and skin repairing effects, comprising a Balkan chrysanthemum leaf extract and ectoine in a mass ratio of 1:5-100.

[0010] The present invention has previously attempted to compound the Balkan chicory leaf extract with Ecdoin to obtain a natural active complex and apply it to cosmetics. It was found that the two can have a synergistic effect, which can significantly improve the moisturizing effect, and at the same time have excellent antioxidant, anti-inflammatory, anti-photoaging, anti-wrinkle and skin repair effects. After research, the synergistic cooperation mechanism is analyzed as follows: the Balkan chicory leaf extract is rich in a large number of carbohydrates of different molecular weights. When biological molecules or cells lose the bound structural water, these carbohydrates can rely on the hydrogen bonds between the charged hydration groups to form a protective film, replacing water molecules to protect biological molecules or cells. At the same time, these carbohydrates can also have the ability to bind water molecules themselves, so they can play a role in locking water. Specifically, when a small part of the small molecule sugars that can enter and pass through the cell membrane enter the cell, a protective film can be formed on the surface of the mitochondria, proteins, etc. in the cell to prevent denaturation, thereby playing a protective role. Most of the remaining larger molecular carbohydrates that cannot pass through the cell membrane form a protective layer on the surface of the cell membrane to play a protective role.

[0011] Ecdoin is a highly hydrophilic substance. When it penetrates into the skin, its molecular structure contains negatively charged carboxyl groups and positively charged cyclic amino groups. The charge distribution on the molecular surface is dense, which can form a strong electrostatic potential. Therefore, it can effectively enrich the water molecules in the surrounding environment of the surrounding cells and combine with water through hydrogen bonds to form stable water complexes. These water complexes surround the cells to form a protective hydration shell, thereby preventing the cells from excessive dehydration and resisting ultraviolet radiation damage to the cells.

[0012] As can be seen from the above, the Balkan chicory leaf extract and Ecdoin in the natural active complex of the present invention can form a protective film or hydration shell inside and outside the cell, respectively, and play a "double" water-locking and moisturizing effect. At the same time, it can protect cells or intracellular substances and effectively resist external oxidative substances, ultraviolet light and other adverse factors. In addition, in addition to sugar substances, the Balkan chicory leaf extract also contains a small amount of Balkan chicory active ingredients and yeast extracts, etc., which also have excellent anti-inflammatory, antioxidant, anti-wrinkle and skin repair effects after compounding with Ecdoin.

[0013] Preferably, the mass ratio of the Balkan choria leaf extract to ectoine is 1:5-50.

[0014] In a second aspect, the present invention provides the use of the above-mentioned natural active compound in the preparation of cosmetics with moisturizing and skin repairing effects.

[0015] Preferably, the cosmetics include but are not limited to facial cream, facial mask liquid, lotion, essence, eye cream, spray, sunscreen, cleansing cream, makeup remover cream, essence oil, liquid foundation, loose powder or lipstick, etc.

[0016] In a third aspect, the present invention provides a facial cream with moisturizing and skin repairing effects, which comprises the following components based on 100 parts by total mass: 20-30 parts of a nanoemulsion containing Balkan moss leaf extract and ectoine, 4-6 parts of cetearyl alcohol, 0.4-0.6 parts of stearic acid glyceryl, 1-2 parts of caprylic capric triglyceride, 4-6 parts of glycerol, 0.1-0.3 parts of carbomer, and 60-75 parts of water.

[0017] In a fourth aspect, the present invention provides a method for preparing a facial cream having moisturizing and skin repairing effects, which comprises the following steps, measured by weight:

[0018] (1) Add 1-5 parts of Balkan lettuce leaf extract to 100 parts of water and stir to dissolve, then heat to 85-95° C., add 0.4-0.6 parts of fatty acid soap, and stir to obtain a mixed solution; then add 1-3 parts of saturated fatty acid methyl ester with a carbon chain length of 7-11, stir to emulsify, then add 0.05-0.15 parts of an alkali catalyst, heat to 110-120° C. and react under reduced pressure for 5-15 hours, dehydrate, cool to 75-85° C., add 80-120 parts of ethyl acetate, heat to reflux, filter, rotary evaporate the obtained filtrate, and vacuum dry to obtain a modified Balkan lettuce leaf extract.

[0019] As mentioned above, the Balkan lettuce leaf extract is rich in a large number of carbohydrates of different molecular weights, but only a small part of the small molecule carbohydrates can pass through the cell membrane into the cell, and most of the larger molecular carbohydrates can only form a protective film outside the cell. Therefore, if the "double" water-locking, moisturizing and protective effects inside and outside the cell are to be further enhanced, more carbohydrates are needed to pass through the cell membrane to protect the biological molecules in the cell. To this end, the present invention modifies the Balkan lettuce leaf extract. The specific principle is: the present invention first dissolves the Balkan lettuce leaf extract in water and then emulsifies it with fatty acid soap, and then adds saturated fatty acid methyl esters with a carbon chain length of 7-11, and utilizes the characteristics of the Balkan lettuce leaf extract that is rich in alcohol hydroxyl groups, and undergoes an ester exchange reaction with saturated fatty acid methyl esters under the action of an alkali catalyst, and the modified Balkan lettuce leaf extract is obtained after separation. After the above-mentioned modification, the surface of the carbohydrate molecules of the Balkan lettuce leaf extract is grafted with saturated fatty acid chains. The saturated fatty acid chains are lipid substances and appear as hydrophobic segments, while the cell membrane is composed of a phospholipid bilayer, with one end of the phospholipid molecule being hydrophilic and the other end being hydrophobic. According to the principle of like dissolves like, the carbohydrates in the unmodified Balkan lettuce leaf extract are mainly hydrophilic substances, so it is difficult for them to pass through the cell membrane (except for some small molecule sugars), while saturated fatty acid chains are more likely to freely enter and exit the cell membrane. Therefore, after modification, the ability of carbohydrates in the Balkan lettuce leaf extract to penetrate the cell membrane has been significantly improved, thereby further improving the ability to protect biological molecules in the cell.

[0020] (2) Add 0.5 parts of modified Balkan lettuce leaf extract and 25-35 parts of caprylic / capric triglyceride to 15-25 parts of polyglycerol-6 caprylate, heat and stir evenly, and then cool to room temperature to obtain an oil phase liquid; dissolve 2.5-5 parts of ectoine in 25-35 parts of water to obtain an aqueous phase liquid; add the aqueous phase liquid dropwise to the oil phase liquid under stirring conditions, and continue stirring until a clear oil-in-water nanoemulsion is formed. The nanoemulsion particles in the oil-in-water nanoemulsion have a core-shell structure, the aqueous phase inner core contains ectoine, and the oil phase outer layer contains the modified Balkan lettuce leaf extract.

[0021] In step (2), the nanoemulsion particles in the water-in-oil nanoemulsion obtained by the present invention have a core-shell structure, the inner core of the aqueous phase contains ectoine, and the outer layer of the oil phase contains a modified Balkan lettuce leaf extract. The advantage of this setting is that it can better enhance the "double" water-locking, moisturizing and protective effects inside and outside the cell. As mentioned above, ectoine is mainly responsible for forming a hydration shell outside the cell, while the modified Balkan lettuce leaf extract is mainly responsible for penetrating into the cell and protecting the biological molecules in the cell. Therefore, the present invention deliberately arranges the modified Balkan lettuce leaf extract in the shell layer of the core-shell structure, and arranges ectoine in the core layer. When the nanoemulsion penetrates into the skin, the modified Balkan lettuce leaf extract in the shell layer is first absorbed and penetrates into the cell. When the shell layer is fully absorbed, the ectoine in the core layer will be released and form a hydration shell outside the cell. If ectoine is placed in the shell layer, it is easy for ectoine to form a hydrated shell before the cell surface and hinder the modified Balkan lettuce leaf extract from entering the cell, thus failing to achieve the aforementioned "double" water-locking, moisturizing and protective effects inside and outside the cell.

[0022] (3) Mix 60-75 parts of water, 4-6 parts of glycerin, and 0.1-0.3 parts of carbomer, and heat and stir to obtain a water phase; mix 1-2 parts of caprylic capric triglyceride, 4-6 parts of cetearyl alcohol, and 0.4-0.6 parts of stearic acid glyceryl, and heat and stir to obtain an oil phase; under heating and stirring conditions, mix the oil phase and the water phase evenly, and cool to room temperature to obtain a cream; mix the obtained cream with 20-30 parts of oil-in-water nanoemulsion, and stir to obtain a facial cream with moisturizing and skin repairing effects.

[0023] Preferably, in step (1), the saturated fatty acid methyl ester with a carbon chain length of 7-11 is methyl octanoate.

[0024] The present invention further discovered through research that the carbon chain length and unsaturation of fatty acid methyl esters have a significant effect on the ability of sugars in the modified Balkan chicory leaf extract to pass through the cell membrane. Finally, we found through experiments that, on the one hand, saturated fatty acid methyl esters have a better modification effect than unsaturated fatty acid methyl esters; on the other hand, fatty acid methyl esters with a carbon chain length of 7-11 and saturation have the highest modification effect. If the carbon chain is too short, it will still not be able to effectively pass through the cell membrane due to the weak hydrophobicity of the modified substance; if the carbon chain is too long, it will significantly increase the molecular volume and hinder the passage of molecules, and may interfere with its ability to distribute to the membrane due to its easier formation of aggregates, resulting in low bioavailability.

[0025] Preferably, in step (1), the base catalyst is sodium ethoxide or potassium carbonate.

[0026] Preferably, in step (1), the fatty acid soap is potassium stearate.

[0027] Preferably, in step (2), during the preparation of the oil phase, the heating and stirring conditions are: temperature 35-45°C, stirring rate 400-600 rpm, and time 10-20 min.

[0028] Preferably, in step (2), during the dropwise addition of the aqueous phase, the stirring condition is a stirring rate of 100-300 rpm.

[0029] Preferably, in step (3), the heating and stirring conditions for the multiple times are: temperature 65-75°C, stirring rate 200-400 rpm, and time 20-40 min.

[0030] Compared with the prior art, the present invention has the following technical effects:

[0031] (1) The present invention is the first to compound Balkan chrysanthemum leaf extract with ectoine to obtain a natural active complex and apply it to cosmetics. It is found that the two can produce a synergistic effect and can form a protective film or hydration shell inside and outside the cells respectively, achieving a "double" water-locking and moisturizing effect, while also having excellent antioxidant, anti-inflammatory, anti-photoaging, anti-wrinkle and skin repair effects.

[0032] (2) The present invention grafts saturated fatty acid chains of specific chain lengths onto the molecular surfaces of carbohydrate substances in the Balkan chicory leaf extract through modification treatment, thereby significantly improving their ability to penetrate cell membranes, thereby further improving their ability to protect intracellular biological molecules.

[0033] (3) The present invention prepares an oil-in-water nanoemulsion containing core-shell structure nanoemulsion particles, wherein ectoine is placed in the inner core of the water phase, and the modified Balkan lettuce leaf extract is placed in the outer layer of the oil phase. This is conducive to the modified Balkan lettuce leaf extract being first absorbed and permeated into the cells. When the shell layer is fully absorbed, the ectoine in the core layer will be released and form a hydrated shell outside the cell, thereby obtaining a better "double" water-locking, moisturizing and protective effect inside and outside the cell. DETAILED DESCRIPTION

[0034] The present invention will be further described below in conjunction with the embodiments.

[0035] Overall embodiment

[0036] A natural active compound with moisturizing and skin repairing effects comprises a Balkan moss leaf extract and ectoine in a mass ratio of 1:5-100 (more preferably 1:5-50).

[0037] The above-mentioned natural active complex can be used to prepare cosmetics with moisturizing and skin repairing effects, including but not limited to face cream, facial mask liquid, lotion, essence, eye cream, spray, sunscreen, cleansing cream, makeup remover cream, essence oil, foundation liquid, loose powder or lipstick, etc.

[0038] A facial cream with moisturizing and skin repairing effects comprises the following components based on 100 parts by total weight: 20-30 parts of a nanoemulsion containing a Balkan chamomile leaf extract and ectoine, 4-6 parts of cetearyl alcohol, 0.4-0.6 parts of stearic acid glyceryl, 1-2 parts of caprylic capric triglyceride, 4-6 parts of glycerol, 0.1-0.3 parts of carbomer, and 60-75 parts of water.

[0039] Preferably, the nanoemulsion is a water-in-oil nanoemulsion, in which the aqueous core of the nanoemulsion particles contains ectoine, and the oily outer layer contains the modified Balkan moss leaf extract.

[0040] A method for preparing a facial cream with moisturizing and skin repairing effects, comprising the following steps, calculated by weight:

[0041] (1) Add 1-5 parts of Balkan moss leaf extract to 100 parts of water and stir to dissolve, then heat to 85-95° C., add 0.4-0.6 parts of fatty acid soap (preferably potassium stearate), and stir to obtain a mixed solution; then add 1-3 parts of saturated fatty acid methyl ester with a carbon chain length of 7-11 (preferably methyl octanoate), stir to emulsify, then add 0.05-0.15 parts of an alkali catalyst (preferably sodium ethanol or potassium carbonate), heat to 110-120° C. and reduce pressure to react for 5-15 hours, dehydrate, cool to 75-85° C., add 80-120 parts of ethyl acetate, heat to reflux, filter with suction, rotary evaporate the obtained filtrate, and vacuum dry to obtain a modified Balkan moss leaf extract.

[0042] (2) Add 0.5 parts of modified Balkan chayote leaf extract and 25-35 parts of caprylic / capric triglyceride to 15-25 parts of polyglycerol-6 caprylate, heat at 35-45°C and stir at 400-600 rpm for 10-20 min, cool to room temperature to obtain an oil phase liquid; dissolve 2.5-5 parts of ectoine in 25-35 parts of water to obtain an aqueous phase liquid; add the aqueous phase liquid dropwise to the oil phase liquid under stirring at 100-300 rpm, and continue stirring until a clear oil-in-water nanoemulsion is formed.

[0043] (3) 60-75 parts of water, 4-6 parts of glycerin and 0.1-0.3 parts of carbomer are mixed, heated to 65-75°C, and stirred at 200-400 rpm for 20-40 minutes to obtain an aqueous phase; 1-2 parts of caprylic capric triglyceride, 4-6 parts of cetearyl alcohol and 0.4-0.6 parts of stearic acid glyceryl are mixed, heated to 65-75°C, and stirred at 200-400 rpm for 20-40 minutes to obtain an oil phase; the oil phase and the aqueous phase are mixed evenly under heating conditions at 65-75°C, stirred at 200-400 rpm for 20-40 minutes, and cooled to room temperature to obtain a cream; the obtained cream is mixed with 20-30 parts of oil-in-water nanoemulsion and stirred evenly to obtain a facial cream with moisturizing and skin repairing effects.

[0044] Examples 1-3 and Comparative Examples 1-2

[0045] In order to study the compounding effect of "Balkan moss leaf extract + ectoine", active substance solutions (using deionized water as solvent) were prepared according to the formulas of Examples 1-3 and Comparative Examples 1-2 in the following table. The source of Balkan moss leaf extract is (UNISURRECTION S-61 / Givaudan), and the source of ectoine is ( Zhongke Xinyang).

[0046] Test Group A: Concentration of Balkan Herbaceous Plant Extract B: Ecdoin concentration Mass ratio (A / B) Example 1 0.500wt% 2.500wt% 1∶5 Example 2 0.333wt% 2.667wt% 1∶8 Example 3 0.273wt% 2.727wt% 1∶10 Comparative Example 1 3.00wt% / / Comparative Example 2 / 3.00wt% /

[0047] Effect test

[0048] The above-prepared active substance solutions of Examples 1-3 and Comparative Examples 1-2 were diluted 30 times and then tested for their effects, as follows: (1) Skin irritation test: About 24 hours before the test, the hair on both sides of the back spine of SD rats (8 weeks old, male) was trimmed without damaging the epidermis. The hair removal range was about 3 cm×3 cm on the left and right. About 0.5 mL of the active substance solution of Examples 1-3 and Comparative Examples 1-2 was applied directly to the skin, and then covered with two layers of gauze (2.5 cm×2.5 cm) and a layer of cellophane or the like, and then fixed with non-irritating adhesive tape and bandage. The skin on the other side was used as a control. A closed test was used with an application time of 4 hours. After the test, the residual test substance was removed with warm water. Observe the skin reaction at the application site 1h, 24h, 48h and 72h after removing the test substance, and score the skin reaction according to the table below. Make a comprehensive evaluation based on the average score of the test animals. Determine the intensity of skin irritation based on the average of the highest scores at each observation time point of 24h, 48h and 72h.

[0049] Skin irritation intensity classification

[0050] Integral mean strength 0～＜0.5 Non-irritating 0.5～＜2.0 Mild irritation 2.0～＜6.0 Moderate irritation 6.0～8.0 Strong irritation

[0051] Results of the test substance's skin irritation test on SD rats

[0052]

[0053] Conclusion: The active substance solutions of Examples 1-3 and Comparative Examples 1-2 have no acute skin irritation to SD rats.

[0054] (2) Moisturizing effect test:

[0055] (2.1) Cell experiment: Immunofluorescence assay to determine the effect of promoting AQP3 protein expression: Aquaporin 3 (AQP3) can promote the transport of endogenous glycerol and triglycerides in the sebaceous glands into the epidermis, affecting the moisturizing effect of the skin. Therefore, upregulating AQP3 can achieve moisturizing effect. The moisturizing effect of the sample to be tested can be evaluated by testing the changes in AQP3 protein expression after the sample is exposed to keratinocytes.

[0056] (a) Test system: Human keratinocytes were subcultured and seeded into multi-well plates and incubated overnight in an incubator (37° C., 5% CO 2 ).

[0057] (b) Solution preparation: Prepare the working solution of the test substance according to the following table:

[0058]

[0059]

[0060] (c) Drug administration: According to the specific experimental design in the above table, when the cell plating rate in the multi-well plate reaches 30-50%, group drug administration is carried out, 1 mL of sample is added to each well, and 3 replicate wells are set for each group. After drug administration, the well plate is placed in an incubator (37°C, 5% CO2) for 24 hours.

[0061] (d) Treatment: After the incubation, discard the old solution, wash 3 times with 1 mL / well PBS, add 1 mL 4% paraformaldehyde to each well to fix the slides at room temperature for 15 min. After fixation, store the multi-well plate at 4°C for later use. Block with sheep serum, incubate with primary and secondary antibodies, and add Hochest33342 for staining.

[0062] (e) Seal the slide for observation and photography: Use a needle to pick out the crawling slice, add a drop of antifade agent on the slide and place the crawling slice upside down on the slide. Take photos under a fluorescence microscope within 24 hours and analyze the test results. The results are shown in the following table:

[0063] Grouping Relative IOD / average cell number Blank control (BC) 1.000 Positive Control (PC) 1.532 Example 1 2.268 Example 2 2.757 Example 3 2.522 Comparative Example 1 1.932 Comparative Example 2 1.738

[0064] From the data in the above table, it can be seen that Examples 1-3 and Comparative Examples 1-2 can significantly increase the expression of AQP3 protein and have excellent moisturizing effects. Among them, the effect of compounding Balkan chicory leaf extract and ectoine in Examples 1-3 is better than that of pure Balkan chicory leaf extract and pure ectoine, indicating that the combination of the two has a synergistic effect, and the effect is best under the ratio of Example 2.

[0065] (3) Antioxidant capacity test:

[0066] (3.1) DPPH free radical scavenging:

[0067] (a) Weigh 20 mg of DPPH and dilute to 250 mL with anhydrous ethanol as a DPPH standard reagent;

[0068] (b) preparing a plurality of active substance anhydrous ethanol solutions with different concentration gradients according to the active substance ratios of Examples 1-3 and Comparative Examples 1-2 to obtain corresponding samples to be tested;

[0069] (c) Set up a blank control group (2 mL DPPH standard reagent + 1 mL anhydrous ethanol) and a test group (2 mL DPPH standard reagent + 1 mL of the sample to be tested).

[0070] (d) After reacting at room temperature for 10 min, the absorbance at 517 nm was measured and the clearance rate was calculated according to the following formula: Clearance rate (%) = (1-A / A0) × 100%.

[0071] Wherein, A: absorbance value of blank control group at 517nm; A: absorbance value of test sample at 517nm.

[0072] The concentration of active substances that scavenges 50% of DPPH free radicals (IC 50 ) were used to characterize the free radical scavenging effects of the active substances in Examples 1-3 and Comparative Examples 1-2. The specific test results are shown in the following table.

[0073] Grouping <![CDATA[IC 50 (μg / mL)]]> Example 1 43.7 Example 2 35.9 Example 3 40.5 Comparative Example 1 56.4 Comparative Example 2 66.3

[0074] It can be seen from the data in the above table that Examples 1-3 and Comparative Examples 1-2 all have excellent free radical scavenging effects. Among them, the effect of compounding the Balkan lettuce leaf extract and Ecdoin in Examples 1-3 is better than that of pure Balkan lettuce leaf extract and pure Ecdoin, indicating that the combination of the two has a synergistic effect, and the effect of compounding the Balkan lettuce leaf extract and Ecdoin at the ratio of Example 2 is the best.

[0075] (4) Anti-inflammatory ability test:

[0076] (4.1) Test of the ability to inhibit inflammatory factors: RAW264.7 cells in the logarithmic growth phase were taken and the cell density was adjusted to 5×10 5 Cells were inoculated into multi-well plates. The cells were divided into 7 groups, as shown in the following table:

[0077] Grouping Processing Normal control group Add 2 mL of cell culture medium LPS intervention group Treatment with 1 μg / mL LPS without active substance intervention Example 1 After pretreatment with the active substance solution of Example 1 for 2 h, 1 μg / mL LPS was added for intervention for 24 h. Example 2 After pretreatment with the active substance solution of Example 2 for 2 h, 1 μg / mL LPS was added for intervention for 24 h. Example 3 After pretreatment with the active substance solution of Example 3 for 2 h, 1 μg / mL LPS was added for intervention for 24 h. Comparative Example 1 After pretreatment with the active substance solution of Comparative Example 1 for 2 h, 1 μg / mL LPS was added for intervention for 24 h. Comparative Example 2 After pretreatment with the active substance solution of Comparative Example 2 for 2 hours, 1 μg / mL LPS was added for intervention for 24 hours.

[0078] After incubation for 24 h, the contents of NO, TNF-α, and IL-6 in the supernatant were detected by flow cytometry. The results are shown in the following table.

[0079]

[0080]

[0081] As can be seen from the data in the above table, the secretion of NO, TNF-α, and IL-6 in the normal control group is at a low level. When LPS is stimulated in the LPS intervention group, the secretion of NO, IL-6, and TNF-α in the cells increases significantly, with significant differences. In Examples 1-3 and Comparative Examples 1-2, after being treated with active substances compounded in different proportions before LPS intervention, the secretion of NO, IL-6, and TNF-α in the cells is significantly lower than that in the LPS intervention group. The results show that the active substances in Examples 1-3 and Comparative Examples 1-2 can inhibit the expression level of proinflammatory factors and have significant anti-inflammatory effects.

[0082] (5) Light damage protection effect test:

[0083] (5.1) Cell experiment: Apoptosis rate test of keratinocytes after ultraviolet (UVB) irradiation: A blank control group was set up, and keratinocytes were cultured with the active substance solutions of Examples 1-3 and Comparative Examples 1-2 for 24 hours, and then irradiated with ultraviolet light (UVB dose of 0-200 mJ / (cm 2 )), the apoptosis rate of keratinocytes induced by ultraviolet light was evaluated by histological staining (H&E), and the results are shown in the following table:

[0084]

[0085] From the data in the table above, we can see that the survival rate of keratinocytes in the blank control group gradually decreased with the gradual increase of UVB dose. 2 )) dose, almost all of the cells were apoptotic, while after treatment with Examples 1-3 and Comparative Examples 1-2, the survival rate of keratinocytes was improved, indicating that the Balkan choria leaf extract alone basically has no anti-ultraviolet light aging effect, while the single ectoine and the combination of the two can effectively protect skin cells from UVB-induced damage.

[0086] (6) Skin repair and anti-wrinkle effect test:

[0087] (5.1) Test on the effect of promoting proliferation of human skin fibroblasts: 6 experimental groups (blank control group, Examples 1-3 and Comparative Examples 1-2) were set up, and the proliferated cells (normal human skin fibroblasts) were dispersed in Dulbecco's MEM medium containing FBS and inoculated into a multi-well culture plate. Then, the cells were cultured in a CO2 incubator (37°C). On the second day after sowing, the culture medium was replaced with Dulbecco's MEM medium containing FBS added with the active solution of Examples 1-3 and Comparative Examples 1-2 (the added amount was 50wt%) and the Dulbecco's MEM medium containing FBS of the blank group, and then cultured in a CO2 incubator. On the last day of culture, the culture medium was discarded, and an MTT solution was prepared in Dulbecco's MEM containing FBS, added and cultured in a CO2 incubator. Afterwards, it was dissolved in the extract and microparticles, and the absorbance was measured with a rate reader and used as a numerical value representing the number of cells. The measurement results were the control, converted to 100, and the results are shown in the following table.

[0088] Grouping The percentage of cells relative to the blank control group Blank control group 100％ Example 1 123.5％ Example 2 119.3％ Example 3 115.2％ Comparative Example 1 126.4％ Comparative Example 2 110.2％

[0089] As can be seen from the data in the above table, the results show that the active substance solutions of Examples 1-3 and Comparative Examples 1-2 have a proliferation-promoting effect on normal human skin fibroblasts, and the trend shows that as the proportion of Balkan moss leaf extract gradually increases, the amount of promotion of human skin fibroblast proliferation also gradually increases.

[0090] (5.2) Test on the effect of promoting the production of type I collagen: 6 experimental groups (blank control group, Examples 1-3 and Comparative Examples 1-2) were set up, and the proliferated cells (normal human skin fibroblasts) were dispersed in Dulbecco's MEM medium containing FBS and inoculated in a multi-well culture plate. Then, the cells were cultured in a CO2 incubator (37°C). On the second day after inoculation, the culture medium was replaced with Dulbecco's MEM medium containing FBS added with active solution of Examples 1-3 and Comparative Examples 1-2 (the added amount was 50wt%) and the blank group, and then cultured in a CO2 incubator. Afterwards, the supernatant was collected and the amount of type I collagen was determined by ELISA. After collecting the supernatant, the amount of type I collagen per unit protein was calculated by lysing the cells, and the results were deduced and evaluated. The results are shown in the following table.

[0091] Grouping Type I collagen content (μg / mL) Blank control group 17.88 Example 1 33.21 Example 2 38.33 Example 3 36.70 Comparative Example 1 35.21 Comparative Example 2 25.72

[0092] As can be seen from the data in the above table, the results show that compared with the blank control group, the active substance solutions of Examples 1-3 and Comparative Examples 1-2 can promote the production of type I collagen, and the effects of Examples 1-3 and Comparative Example 1 are better than those of Comparative Example 2. Type I collagen has a significant relationship with the elasticity and strength of the skin, so the active substance solution of the present invention has the effect of improving skin elasticity and wrinkles.

[0093] Example 4 (Modified Balkan Herb Leaf Extract + Ecdoin)

[0094] A facial cream with modified Balkan chamomile leaf extract and ectoine as active ingredients comprises the following components based on 100 parts by total mass: 25 parts of water-in-oil nanoemulsion containing modified Balkan chamomile leaf extract and ectoine, 5 parts of cetearyl alcohol, 0.5 parts of glyceryl stearate, 1.5 parts of caprylic capric triglyceride, 5 parts of glycerol, 0.2 parts of carbomer and 62.8 parts of deionized water.

[0095] The preparation of the above-mentioned facial cream comprises the following steps:

[0096] (1) 3 g of Balkan lettuce leaf extract (UNISURRECTION S-61 / Givaudan) was added to a three-necked flask containing 100 g of deionized water and stirred until the Balkan lettuce leaf extract was dissolved, then heated to 90° C. in an oil bath, 0.5 g of potassium stearate was added, and the mixture was stirred for 15 min to obtain a mixed solution; 2 g of methyl octanoate was added to the mixed solution, stirred and emulsified, and then 0.1 g of sodium ethoxide was added, the mixture was heated to 115° C. and reacted under reduced pressure for 10 h, dehydrated, cooled to 80° C., 100 g of ethyl acetate was slowly added, heated to reflux, and filtered, the obtained filtrate was concentrated by rotary evaporation of the solvent, and the concentrate was placed in a vacuum drying oven and vacuum dried for 4 h, and taken out to obtain a modified Balkan lettuce leaf extract.

[0097] (2) 0.5 g of modified Balkan chayote leaf extract and 30 g of caprylic / capric triglyceride were added to a beaker containing 20 g of polyglycerol-6 caprylate, heated at 40°C and stirred at 500 rpm for 15 min, and cooled to room temperature to obtain an oil phase liquid. 4 g of ectoin ( Zhongke Xinyang) was dissolved in a beaker containing 30g of deionized water to obtain an aqueous phase liquid. The aqueous phase liquid was added dropwise to the oil phase liquid under stirring at 200rpm, and stirring was continued until a clear water-in-oil nanoemulsion was formed.

[0098] (3) 62.8 g of deionized water, 5 g of glycerol, and 0.2 g of carbomer were mixed in a beaker, heated to 70°C in a water bath, and stirred at 300 rpm for 30 min to obtain an aqueous phase. 1.5 g of caprylic capric triglyceride, 5 g of cetearyl alcohol, and 0.5 g of stearic acid glyceryl were mixed in a beaker, heated to 70°C, and stirred at 300 rpm for 30 min to obtain an oil phase. The oil phase and the aqueous phase were mixed in a large beaker under heating conditions of 70°C, stirred at 300 rpm for 30 min, and cooled to room temperature to obtain a cream; the obtained cream was mixed with 25 g of oil-in-water nanoemulsion and stirred evenly to obtain a facial cream.

[0099] Comparative Example 3 (Trehalose+Ectoin, the difference from Example 4 is that an equal amount of trehalose is used to replace the modified Balkan moss leaf extract)

[0100] A facial cream with trehalose and ectoine as active substances comprises the following components based on 100 parts by total weight: 25 parts of water-in-oil nanoemulsion containing trehalose and ectoine, 5 parts of cetearyl alcohol, 0.5 parts of glyceryl stearate, 1.5 parts of caprylic capric triglyceride, 5 parts of glycerol, 0.2 parts of carbomer and 62.8 parts of deionized water.

[0101] The preparation of the above-mentioned facial cream comprises the following steps:

[0102] (1) 30 g of caprylic / capric triglyceride was added to a beaker containing 20 g of polyglycerol-6 caprylate, heated at 40°C and stirred at 500 rpm for 15 min, and cooled to room temperature to obtain an oil phase liquid. 0.5 g of trehalose (TREHA, Hayashibara Co., Ltd.) and 4 g of ectoin ( Zhongke Xinyang) was dissolved in a beaker containing 30g of deionized water to obtain an aqueous phase liquid. The aqueous phase liquid was added dropwise to the oil phase liquid under stirring at 200rpm, and stirring was continued until a clear water-in-oil nanoemulsion was formed (trehalose and ectoine were both located in the aqueous phase core).

[0103] (2) Same as Example 4.

[0104] Comparative Example 4 (Balkan chamomile leaf extract + Ecdoin, the difference from Example 4 is that the Balkan chamomile leaf extract has not been modified)

[0105] A facial cream with Balkan lettuce leaf extract and ectoine as active ingredients comprises the following components based on 100 parts by total mass: 25 parts of water-in-oil nanoemulsion containing Balkan lettuce leaf extract and ectoine, 5 parts of cetearyl alcohol, 0.5 parts of glyceryl stearate, 1.5 parts of caprylic capric triglyceride, 5 parts of glycerol, 0.2 parts of carbomer and 62.8 parts of deionized water.

[0106] The preparation of the above-mentioned facial cream comprises the following steps:

[0107] (1) 30 g of caprylic / capric triglyceride was added to a beaker containing 20 g of polyglycerol-6 caprylate, heated at 40°C and stirred at 500 rpm for 15 min, and cooled to room temperature to obtain an oil phase. 0.5 g of Balkan moss leaf extract (UNISURRECTION S-61 / Givaudan) and 4 g of ectoin ( Zhongke Xinyang) was dissolved in a beaker containing 30g of deionized water to obtain an aqueous phase liquid. The aqueous phase liquid was added dropwise to the oil phase liquid under stirring at 200rpm, and stirring was continued until a clear water-in-oil nanoemulsion was formed (because the unmodified Balkan lettuce leaf extract is a hydrophilic substance, it and Ecdoin are both located in the aqueous phase core).

[0108] (2) Same as Example 4.

[0109] Comparative Example 5 (modified Balkan chamomile leaf extract + ectoine, the difference from Example 4 is that the Balkan chamomile leaf extract is modified by using methyl butyrate with a shorter carbon chain)

[0110] A facial cream with modified Balkan chamomile leaf extract and ectoine as active ingredients comprises the following components based on 100 parts by total mass: 25 parts of water-in-oil nanoemulsion containing modified Balkan chamomile leaf extract and ectoine, 5 parts of cetearyl alcohol, 0.5 parts of glyceryl stearate, 1.5 parts of caprylic capric triglyceride, 5 parts of glycerol, 0.2 parts of carbomer and 62.8 parts of deionized water.

[0111] The preparation of the above-mentioned facial cream comprises the following steps:

[0112] (1) 3 g of Balkan lettuce leaf extract (UNISURRECTION S-61 / Givaudan) was added to a three-necked flask containing 100 g of deionized water and stirred until the Balkan lettuce leaf extract was dissolved, then heated to 90° C. in an oil bath, 0.5 g of potassium stearate was added, and the mixture was stirred for 15 min to obtain a mixed solution; 2 g of methyl butyrate was added to the mixed solution, stirred and emulsified, and then 0.1 g of sodium ethoxide was added, the mixture was heated to 115° C. and reacted under reduced pressure for 10 h, dehydrated, cooled to 80° C., 100 g of ethyl acetate was slowly added, heated to reflux, and filtered, the obtained filtrate was concentrated by rotary evaporation of the solvent, and the concentrate was placed in a vacuum drying oven and vacuum dried for 4 h, and taken out to obtain a modified Balkan lettuce leaf extract.

[0113] (2) Same as Example 4.

[0114] (3) Same as Example 4.

[0115] Comparative Example 6 (modified Balkan lettuce leaf extract + ectoine, the difference from Example 4 is that the Balkan lettuce leaf extract is modified by using animal oleic acid methyl ester with a longer carbon chain)

[0116] A facial cream with modified Balkan chamomile leaf extract and ectoine as active ingredients comprises the following components based on 100 parts by total mass: 25 parts of water-in-oil nanoemulsion containing modified Balkan chamomile leaf extract and ectoine, 5 parts of cetearyl alcohol, 0.5 parts of glyceryl stearate, 1.5 parts of caprylic capric triglyceride, 5 parts of glycerol, 0.2 parts of carbomer and 62.8 parts of deionized water.

[0117] The preparation of the above-mentioned facial cream comprises the following steps:

[0118] (1) 3 g of Balkan lettuce leaf extract (UNISURRECTION S-61 / Givaudan) was added to a three-necked flask containing 100 g of deionized water and stirred until the Balkan lettuce leaf extract was dissolved, then heated to 90°C in an oil bath, 0.5 g of potassium stearate was added, and the mixture was stirred for 15 min to obtain a mixed solution; 2 g of animal oleic acid methyl ester was added to the mixed solution, stirred and emulsified, and then 0.1 g of sodium ethanol was added, the temperature was raised to 115°C and the reaction was carried out under reduced pressure for 10 h, dehydrated, cooled to 80°C, 100 g of ethyl acetate was slowly added, heated to reflux, and filtered, the obtained filtrate was concentrated by rotary evaporation of the solvent, and the concentrate was placed in a vacuum drying oven for vacuum drying for 4 h, and taken out to obtain a modified Balkan lettuce leaf extract.

[0119] (2) Same as Example 4.

[0120] (3) Same as Example 4.

[0121] Comparative Example 7 (modified Balkan chamomile leaf extract + ectoine, the difference from Example 4 is that the nanoemulsion is an oil-in-water type)

[0122] A facial cream with modified Balkan chamomile leaf extract and ectoine as active ingredients comprises the following components based on 100 parts by total mass: 25 parts of water-in-oil nanoemulsion containing modified Balkan chamomile leaf extract and ectoine, 5 parts of cetearyl alcohol, 0.5 parts of glyceryl stearate, 1.5 parts of caprylic capric triglyceride, 5 parts of glycerol, 0.2 parts of carbomer and 62.8 parts of deionized water.

[0123] The preparation of the above-mentioned facial cream comprises the following steps:

[0124] (1) Same as Example 4.

[0125] (2) 0.5 g of modified Balkan chayote leaf extract and 30 g of caprylic / capric triglyceride were added to a beaker containing 20 g of polyglycerol-6 caprylate, heated at 40°C and stirred at 500 rpm for 15 min, and cooled to room temperature to obtain an oil phase liquid. 4 g of ectoin ( Zhongke Xinyang) was dissolved in a beaker containing 30g of deionized water to obtain an aqueous phase. The oil phase was added dropwise to the aqueous phase under stirring at 200rpm, and stirring was continued until a clear oil-in-water nanoemulsion was formed.

[0126] (3) Same as Example 4.

[0127] Effect test

[0128] The effects of the creams prepared in Example 4 and Comparative Examples 3-7 were tested as follows:

[0129] (1) Skin irritation test: About 24 hours before the test, the hair on both sides of the back spine of SD rats (8 weeks old, male) was cut off without damaging the epidermis. The hair removal range was about 3 cm×3 cm on the left and right sides. About 0.5 mL of the cream of Example 4 and Comparative Examples 3-7 was applied directly on the skin, and then covered with two layers of gauze (2.5 cm×2.5 cm) and a layer of cellophane or the like, and then fixed with non-irritating adhesive tape and bandage. The skin on the other side was used as a control. A closed test was used, and the application time was 4 hours. After the test, the residual test substance was removed with warm water. The skin reaction of the application site was observed 1 hour, 24 hours, 48 ​​hours and 72 hours after the removal of the test substance, and the skin reaction score was calculated according to the following table. The average score of the test animal was used for comprehensive evaluation. The skin irritation intensity was determined based on the average of the highest score at each observation time point of 24 hours, 48 ​​hours and 72 hours.

[0130] Skin irritation intensity classification

[0131] Integral mean strength 0～＜0.5 Non-irritating 0.5～＜2.0 Mild irritation 2.0～＜6.0 Moderate irritation 6.0～8.0 Strong irritation

[0132] Results of the test substance's skin irritation test on SD rats

[0133]

[0134] Conclusion: The creams of Example 4 and Comparative Examples 3-7 have no acute skin irritation to SD rats.

[0135] (2) Human testing:

[0136] 70 healthy Chinese female subjects who considered themselves to be sensitive skin and had dry and rough facial skin, poor barrier function, loose and inelastic facial skin, and obvious fine lines on the face were selected. The age range was 40-50 years old and they were divided into 7 groups on average. Each group used the cream of Example 4 and Comparative Examples 3-7 and the blank control method to test the moisture content of the stratum corneum of the test part of the subject before use, 2 hours, 4 hours, and 8 hours after use. The method of before and after control was used to test the moisture content of the stratum corneum of the test part of the subject, the transepidermal water loss value of the skin, the roughness of the skin, and the elasticity of the skin before and after using the cream for 14 days and 28 days (Note: the ambient temperature during the test was 20.2-21.9°C, and the relative humidity was 47-58%RH: the skin roughness and skin elasticity items were only tested in Example 4).

[0137]

[0138]

[0139] The test results are as follows:

[0140] (2.1) Skin stratum corneum moisture content test (short-term):

[0141] Grouping Improvement rate after 2 hours of use Improvement rate 4 hours after use Improvement rate after 8 hours of use Blank control -0.23％ -0.56％ -3.23％ Example 4 72.92％ 62.87％ 47.22％ Comparative Example 3 57.23％ 40.32％ 26.45％ Comparative Example 4 62.38％ 49.77％ 31.21％ Comparative Example 5 68.38％ 56.58％ 38.56％ Comparative Example 6 67.52％ 56.88％ 36.97％ Comparative Example 7 61.21％ 47.12％ 28.44％

[0142] (2.2) Skin stratum corneum moisture content test (long-term):

[0143]

[0144]

[0145] (2.3) Skin Transepidermal Water Loss Test:

[0146] Grouping Improvement rate after 14 days of use Improvement rate after 28 days of use Example 4 28.44％ 30.12％ Comparative Example 3 18.78％ 19.57％ Comparative Example 4 21.12％ 20.98％ Comparative Example 5 24.56％ 25.12％ Comparative Example 6 23.87％ 25.01％ Comparative Example 7 20.59％ 21.21％

[0147] It can be seen from the data in the above table that, whether in the short-term / long-term or skin transepidermal water loss test, compared with the blank control group, Example 4 and Comparative Examples 3-7 can significantly improve the skin's moisturizing ability. Among them, Example 4 has the best effect. Compared with Example 4, since Comparative Example 3 replaces the modified Balkan chicory leaf extract with trehalose, Comparative Example 4 does not modify the Balkan chicory leaf extract, Comparative Examples 5 / 6 are modified with shorter / longer carbon chains, respectively, and Comparative Example 7 does not use oil-in-water nanoemulsion, the moisturizing effect is affected to a certain extent.

[0148] (2.4) Skin roughness SEr test:

[0149] Grouping Base value (D0) Use 14 day value Improvement rate after 14 days of use Use 28 day value Improvement rate after 28 days of use Example 4 3.52 4.18 18.75％ 4.68 32.95％

[0150] (2.5) Skin elasticity R2 test:

[0151] Grouping Base value (D0) Use 14 day value Improvement rate after 14 days of use Use 28 day value Improvement rate after 28 days of use Example 4 0.56 0.62 10.71％ 0.64 14.29％

[0152] (2.6) Skin elasticity R5 test:

[0153] Grouping Base value (D0) Use 14 day value Improvement rate after 14 days of use Use 28 day value Improvement rate after 28 days of use Example 4 0.46 0.50 8.70％ 0.54 17.39％

[0154] It can be seen from the data in the above table that the facial cream of Example 4 can significantly improve skin roughness and skin elasticity, and thus has good anti-wrinkle and skin repair capabilities, can reduce the fine lines of the user, and make the skin firmer and more elastic.

[0155] Example 5

[0156] A facial cream with modified Balkan chamomile leaf extract and ectoine as active ingredients comprises the following components based on 100 parts by total weight: 20 parts of a nanoemulsion containing modified Balkan chamomile leaf extract and ectoine, 4 parts of cetearyl alcohol, 0.4 parts of glyceryl stearate, 1 part of caprylic capric triglyceride, 4 parts of glycerol, 0.1 parts of carbomer and 70.5 parts of deionized water.

[0157] The method for preparing the above-mentioned facial cream with moisturizing and skin repairing effects comprises the following steps:

[0158] (1) 2 g of Balkan lettuce leaf extract (UNISURRECTION S-61 / Givaudan) was added to a three-necked flask containing 100 g of deionized water and stirred until the Balkan lettuce leaf extract was dissolved, then heated to 90° C. in an oil bath, 0.4 g of potassium stearate was added, and the mixture was stirred for 15 min to obtain a mixed solution; 1.5 g of methyl octanoate was added to the mixed solution, stirred and emulsified, and then 0.05 g of sodium ethoxide was added, the temperature was raised to 110° C. and the reaction was carried out under reduced pressure for 15 h, dehydrated, cooled to 80° C., 80 g of ethyl acetate was slowly added, heated to reflux, and filtered, and the obtained filtrate was concentrated by rotary evaporation of the solvent, and then the concentrate was placed in a vacuum drying oven and vacuum dried for 4 h, and taken out to obtain a modified Balkan lettuce leaf extract.

[0159] (2) 0.5 g of modified Balkan chayote leaf extract and 25 g of caprylic / capric triglyceride were added to a beaker containing 15 g of polyglycerol-6 caprylate, heated at 45°C and stirred at 400 rpm for 20 min, and cooled to room temperature to obtain an oil phase liquid. 2.5 g of ectoine ( Zhongke Xinyang) was dissolved in a beaker containing 25g of deionized water to obtain an aqueous phase liquid. The aqueous phase liquid was added dropwise to the oil phase liquid under stirring at 100rpm, and stirring was continued until a clear water-in-oil nanoemulsion was formed.

[0160] (3) 70.5 g of deionized water, 4 g of glycerol, and 0.1 g of carbomer were mixed in a beaker, heated to 65° C. in a water bath, and stirred at 200 rpm for 40 min to obtain an aqueous phase. 1 g of caprylic capric triglyceride, 4 g of cetearyl alcohol, and 0.4 g of stearic acid glyceryl were mixed in a beaker, heated to 65° C., and stirred at 200 rpm for 40 min to obtain an oil phase. The oil phase and the aqueous phase were mixed in a large beaker under heating conditions of 65° C., stirred at 200 rpm for 40 min, and cooled to room temperature to obtain a cream; the obtained cream was mixed with 20 g of oil-in-water nanoemulsion and stirred evenly to obtain a facial cream.

[0161] Example 6

[0162] A facial cream with modified Balkan chamomile leaf extract and ectoine as active ingredients comprises the following components based on 100 parts by total mass: 29 parts of a nanoemulsion containing modified Balkan chamomile leaf extract and ectoine, 4 parts of cetearyl alcohol, 0.5 parts of glyceryl stearate, 2 parts of caprylic capric triglyceride, 4 parts of glycerol, 0.2 parts of carbomer and 60.3 parts of deionized water.

[0163] The method for preparing the above-mentioned facial cream with moisturizing and skin repairing effects comprises the following steps:

[0164] (1) 5 g of Balkan lettuce leaf extract (UNISURRECTION S-61 / Givaudan) was added to a three-necked flask containing 100 g of deionized water and stirred until the Balkan lettuce leaf extract was dissolved, then heated to 95° C. in an oil bath, 0.6 g of potassium stearate was added, and the mixture was stirred for 15 min to obtain a mixed solution; 3 g of methyl octanoate was added to the mixed solution, stirred and emulsified, and then 0.15 g of sodium ethoxide was added, the temperature was raised to 115° C. and the reaction was carried out under reduced pressure for 10 h, dehydrated, cooled to 80° C., 120 g of ethyl acetate was slowly added, heated to reflux, and filtered, and the obtained filtrate was concentrated by rotary evaporation of the solvent, and then the concentrate was placed in a vacuum drying oven and vacuum dried for 4 h, and taken out to obtain a modified Balkan lettuce leaf extract.

[0165] (2) 0.5 g of modified Balkan chayote leaf extract and 35 g of caprylic / capric triglyceride were added to a beaker containing 25 g of polyglycerol-6 caprylate, heated at 45°C and stirred at 600 rpm for 10 min, and cooled to room temperature to obtain an oil phase liquid. 5 g of ectoin ( Zhongke Xinyang) was dissolved in a beaker containing 35g of deionized water to obtain an aqueous phase liquid. The aqueous phase liquid was added dropwise to the oil phase liquid under stirring at 300rpm, and stirring was continued until a clear water-in-oil nanoemulsion was formed.

[0166] (3) 60.3 g of deionized water, 4 g of glycerol, and 0.2 g of carbomer were mixed in a beaker, heated to 75°C in a water bath, and stirred at 400 rpm for 20 min to obtain an aqueous phase. 2 g of caprylic capric triglyceride, 4 g of cetearyl alcohol, and 0.5 g of stearic acid glyceryl were mixed in a beaker, heated to 75°C, and stirred at 400 rpm for 20 min to obtain an oil phase. The oil phase and the aqueous phase were mixed in a large beaker under heating conditions of 75°C, stirred at 400 rpm for 20 min, and cooled to room temperature to obtain a cream; the obtained cream was mixed with 29 g of oil-in-water nanoemulsion and stirred evenly to obtain a facial cream.

[0167] The raw materials and equipment used in the present invention, unless otherwise specified, are all commonly used raw materials and equipment in the art; the methods used in the present invention, unless otherwise specified, are all conventional methods in the art.

[0168] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent transformation made to the above embodiment based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A facial cream with moisturizing and skin repairing effects, characterized in that: The composition comprises the following components based on 100 parts by total weight: 20-30 parts of water-in-oil nanoemulsion, 4-6 parts of cetearyl alcohol, 0.4-0.6 parts of stearic acid glyceryl, 1-2 parts of caprylic acid capric acid triglyceride, 4-6 parts of glycerol, 0.1-0.3 parts of carbomer, and 60-75 parts of water; The nanoemulsion particles in the water-in-oil nanoemulsion present a core-shell structure, wherein the water phase core contains ectoine, and the oil phase outer layer contains modified Balkan lettuce leaf extract; the surface of the carbohydrate molecules in the modified Balkan lettuce leaf extract is grafted with saturated fatty acid methyl esters with a carbon chain length of 7-11, and the Balkan lettuce leaf extract is dissolved in water, emulsified with fatty acid soap, and subjected to ester exchange reaction with saturated fatty acid methyl esters under the action of an alkali catalyst and separated.

2. The facial cream according to claim 1, characterized in that: The mass ratio of the Balkan chorion leaf extract to ectoine is 1:5-100.

3. The facial cream according to claim 2, characterized in that: The mass ratio of the Balkan chorion leaf extract to ectoine is 1:5-50.

4. A method for preparing the facial cream as claimed in claim 1, characterized in that: By weight, the method comprises the following steps: (1) Add 1-5 parts of Balkan moss leaf extract to 100 parts of water and stir to dissolve, then heat to 85-95°C, add 0.4-0.6 parts of fatty acid soap, stir to obtain a mixed solution; add 1-3 parts of saturated fatty acid methyl ester with a carbon chain length of 7-11, stir to emulsify, then add an alkali catalyst, heat to 110-120°C and react under reduced pressure, dehydrate, cool to 75-85°C, add ethyl acetate, heat to reflux, filter, rotary evaporate the obtained filtrate, and vacuum dry to obtain a modified Balkan moss leaf extract; (2) adding 0.5 parts of modified Balkan chayote leaf extract and 25-35 parts of caprylic / capric triglyceride to 15-25 parts of polyglycerol-6 caprylate, heating and stirring evenly, and then cooling to room temperature to obtain an oil phase liquid; dissolving 2.5-5 parts of ectoine in 25-35 parts of water to obtain an aqueous phase liquid; adding the aqueous phase liquid dropwise to the oil phase liquid under stirring conditions, and continuing stirring until a clear water-in-oil nanoemulsion is formed; (3) Mix 60-75 parts of water, 4-6 parts of glycerin and 0.1-0.3 parts of carbomer, heat and stir to obtain a water phase; mix 1-2 parts of caprylic capric triglyceride, 4-6 parts of cetearyl alcohol and 0.4-0.6 parts of stearic acid glyceryl, heat and stir to obtain an oil phase; under heating and stirring conditions, mix the oil phase and the water phase evenly, and cool to room temperature to obtain a cream; mix the obtained cream with 20-30 parts of oil-in-water nanoemulsion and stir to obtain a facial cream with moisturizing and skin repairing effects.

5. The method for preparing the facial cream according to claim 4, characterized in that: In step (1), the saturated fatty acid methyl ester with a carbon chain length of 7-11 is methyl octanoate.

6. The method for preparing the facial cream according to claim 4, characterized in that: In step (1), the base catalyst is sodium ethoxide or potassium carbonate.

7. The method for preparing the facial cream according to claim 4, characterized in that: In step (1), in step (1), the fatty acid soap is potassium stearate.

8. The method for preparing the facial cream according to claim 4, characterized in that: In step (2), During the preparation of the oil phase, the heating and stirring conditions are: temperature 35-45°C, stirring rate 400-600 rpm, time 10-20 min; During the dropwise addition of the aqueous phase, the stirring condition is a stirring rate of 100-300 rpm.

9. The method for preparing the facial cream according to claim 4, characterized in that: In step (3), the heating and stirring conditions for the several times are: temperature 65-75°C, stirring rate 200-400 rpm, and time 20-40 min.

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