A compound combination for repelling cotton aphids, cotton aphid repellent and its uses

Abstract

The present invention relates to the field of cotton aphid control, and in particular to a compound combination for repelling cotton aphids, a cotton aphid repellent and uses. In the compound combination for repelling cotton aphids, the effective components for repelling cotton aphids include diallyl disulfide and propyl disulfide. The present invention finds that diallyl disulfide and propyl disulfide both have obvious repellent effects on cotton aphids, and the combination of diallyl disulfide and propyl disulfide can be used as a cotton aphid repellent and a cotton aphid repellent, which provides a new way for environmentally friendly and efficient prevention and control of cotton aphids that harm crops such as cotton, and is of great significance for the prevention and control of cotton aphids in crop fields, and has broad economic, social and ecological benefits.

Description

Technical Field

[0001] The invention relates to the field of cotton aphid control, and in particular to a compound combination for repelling cotton aphid, a cotton aphid repellent and uses thereof. Background Art

[0002] Cotton is an important economic crop. Aphis gossypii is a worldwide cotton pest. It can also harm crops such as pomegranate, prickly ash, hibiscus, and melons. Aphids mainly suck plant sap and secrete honeydew, which affects the photosynthesis of leaves. Aphids can also act as vectors to spread a variety of viral diseases, resulting in reduced cotton yield and quality.

[0003] At present, the main means of controlling cotton aphids is spraying chemical pesticides. Due to the irrational use of chemical pesticides, cotton aphids have developed varying degrees of resistance to organophosphorus, organochlorine, carbamate, pyrethroid and neonicotinoid insecticides. In addition, the use of chemical pesticides will also lead to the resurgence of pests and cause huge pollution to the environment. Therefore, it is extremely urgent to reduce the dosage of chemical pesticides and choose alternative safe and effective methods to control cotton aphids.

[0004] Under the current concept of green agricultural development, plant-derived repellents are widely developed and applied because they are environmentally friendly and can alleviate the problem of resistance to chemical pesticides. In view of the plant volatiles that have a repellent effect on cotton aphids, using them as active ingredients to create cotton aphid repellents has important practical significance for the prevention and control of cotton aphids in cotton and other host crop fields. The present invention provides a plant volatile with better repellent effect on cotton aphids and a combination thereof, which is a key problem to be solved in the field. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a compound combination for repelling cotton aphids, a repellent and uses thereof, so as to achieve the effect of utilizing plant-derived volatiles to achieve an environmentally friendly and efficient effect of repelling cotton aphids.

[0006] In a first aspect, the present invention provides a compound combination for repelling cotton aphids, wherein the effective component for repelling cotton aphids comprises diallyl disulfide and propyl disulfide.

[0007] The invention finds that the combined use of diallyl disulfide and propyl disulfide has a significant repellent effect on cotton aphids. The combination of diallyl disulfide and propyl disulfide can be used as a repellent for cotton aphids, providing a new idea and method for the comprehensive prevention and control of cotton aphids.

[0008] Preferably, the volume ratio of diallyl disulfide to propyl disulfide is 1:0.3 to 1:2.

[0009] More preferably, the volume ratio of diallyl disulfide to propyl disulfide is 1:1 to 1:2.

[0010] In a second aspect, the cotton aphid repellent provided by the present invention comprises the compound combination for repelling cotton aphids.

[0011] Preferably, in the cotton aphid repellent, the effective component is a mixture of diallyl disulfide and propyl disulfide, and the volume ratio of diallyl disulfide to propyl disulfide is 1:0.3 to 1:2, preferably 1:1 to 1:2.

[0012] More preferably, the cotton aphid repellent is a solution containing the effective component, and the solvent of the solution is n-hexane, ether or acetone, preferably n-hexane.

[0013] More preferably, in the solution, the ratio of diallyl disulfide and propyl disulfide to the solvent is 5-15 μg:1 μL.

[0014] More preferably, the ratio of diallyl disulfide and propyl disulfide to n-hexane in the solution is 10 μg:1 μL.

[0015] In a third aspect, the present invention provides use of diallyl disulfide and propyl disulfide in preparing a cotton aphid repellent product.

[0016] Preferably, the cotton aphid repellent product is a cotton aphid repellent or a cotton aphid repellent device.

[0017] In the present invention, the structural formula of diallyl disulfide is as follows:

[0018]

[0019] The structural formula of propyl disulfide is as follows:

[0020]

[0021] The beneficial effect of the present invention is at least that: the present invention finds that both diallyl disulfide and propyl disulfide have obvious repellent effects on cotton aphids, and the use of the combination of diallyl disulfide and propyl disulfide as a cotton aphid repellent and the cotton aphid repellent provide a new way for environmentally friendly, better and more efficient prevention and control of cotton aphids that harm crops such as cotton, which is of great significance for the prevention and control of cotton aphids in crop fields and has broad economic, social and ecological benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention and the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 GC-EAD record of cotton aphid for diallyl disulfide.

[0024] Figure 2 GC-EAD record for cotton aphid p-propyl disulfide.

[0025] Figure 3 This is a statistical chart showing the behavioral responses of cotton aphids to diallyl disulfide and propyl disulfide.

[0026] Figure 4 This is a statistical chart showing the repellent effect of diallyl disulfide on cotton aphids.

[0027] Figure 5 This is a statistical chart showing the results of the repellent effect of propyl disulfide on cotton aphids. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the embodiments of the invention clearer, the technical solution in the embodiments of the invention is clearly and completely described below in conjunction with the drawings in the embodiments of the invention. Obviously, the described embodiments are part of the embodiments of the invention, not all of the embodiments. Based on the embodiments of the invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the invention.

[0029] Unless otherwise specified, the raw materials and reagents used in the following examples are all commercially available products or can be prepared by known methods. If no specific techniques or conditions are specified in the examples, they are all conventional methods or the techniques or conditions described in the literature in this field, or according to the product instructions. If the manufacturers of the reagents and instruments are not specified, they are all conventional products that can be purchased through regular channels.

[0030] The present invention provides the use of diallyl disulfide and propyl disulfide as components of a cotton aphid repellent, wherein the effective components of the cotton aphid repellent contain diallyl disulfide and propyl disulfide. The effective component refers to a component that exerts a repellent effect, which is relative to a solvent without a repellent effect. All components of the repellent are conventional commercial products. The uses include: the use of diallyl disulfide and propyl disulfide for preparing a cotton aphid repellent product, which can be a repellent or a repellent device; the use of diallyl disulfide and propyl disulfide for repelling cotton aphids, which can be directly utilizing a single volatile substance of diallyl disulfide or propyl disulfide to exert its effect, or can be utilized in the form of a composition. In short, as long as various direct or indirect uses that utilize the function of diallyl disulfide or propyl disulfide to repel cotton aphids belong to the technical solution to be protected by the present invention.

[0031] The following examples are provided to further illustrate the present invention, rather than to limit the present invention.

[0032] Example 1: Collection of garlic and onion volatiles and screening of active components in garlic and onion volatiles that react to cotton aphids by gas chromatography-electroantennantaneous diffusion (GC-EAD)

[0033] Test method: Put garlic seedlings / onion seedlings in a glass jar. The lower port of the glass jar is connected to the adsorbent and activated carbon through a silicone tube to filter the air and ensure that the air enters the glass tube in a pure manner. The upper port of the glass jar is connected to the adsorption column, and 40 mg of adsorbent is placed in the adsorption column. The silicone tube of the adsorbent at the upper port of the glass jar is connected to the air inlet on the atmospheric sampler. The air enters the glass jar after being filtered by activated carbon and adsorbent, and then the gas with volatiles of garlic seedlings / onion seedlings is pushed into the adsorption column, and the remaining air flow enters the air inlet of the atmospheric sampler. The collection time is 12h, and the gas flow rate is set to 1L / min. After the collection is completed, the adsorption column is first eluted with 500μL of chromatographically pure n-hexane, stored in a 1.5mL sample bottle, and stored in a -20℃ refrigerator. GC-EAD is used to screen the active ingredients in garlic and onion volatiles that react to cotton aphids. It is carried out in a laboratory with a temperature of 23±1℃ and a humidity of 60%±5%. Turn on GC-EAD before the experiment and call the running method. While waiting for the program to heat up, use a small brush to take a test insect, use a blade to quickly cut along the head of the cotton aphid under a microscope, cut off one antenna, cut off the tip of the remaining antenna, and gently attach one end of the head with a capillary filled with 0.9% sodium chloride injection. On the micro-operation table, connect the head to the reference electrode, and connect the end of the antenna to the recording electrode equipped with a capillary by adjusting the knob of the micro-operation table. The reference electrode and recording electrode connected by silver wire are connected to the GC-EAD system. Move the micro-operation table to the sample outlet of the gas chromatograph, start recording the antenna potential signal, and after testing the antenna potential signal to be stable, click the single injection analysis program, insert the SPME device of the volatile sample collected in advance into the gas chromatograph, push out the extraction wire, and start the single injection analysis program. At the same time, start the V4.6.1 software of GC-EAD to record and analyze the FID and EAD data. After recording and analyzing for 10 minutes, pull out the SPME device and the program continues to run. The winged aphids of cotton aphids were repeated more than 3 times. After the EAD peak appeared 3 times, it was determined that it was a substance with electrophysiological activity to cotton aphids. The active substances were analyzed by gas chromatography-mass spectrometry. Program temperature rise: initial temperature 50℃, hold for 1min; then increase to 180℃ at 5℃ / min, hold for 2min; continue to increase to 230℃ at 10℃ / min, hold for 2min. The MS injection port temperature was 230℃, the ion source temperature was 230℃, the ionization current was 70eV; the scanning range was m / z 35-300. The analysis and identification of the active components of volatiles were retrieved by NIST14 spectral library, and compared with the standard mass spectrum and its retention time to determine the main components of the volatiles.

[0034] Test results: Figure 1-2 As shown, diallyl disulfide in garlic volatiles caused antennal responses, and propyl disulfide in onion volatiles caused antennal responses.

[0035] Example 2: Cage test to verify the effect of diallyl disulfide and propyl disulfide on the behavior of cotton aphid

[0036] Test method: Place a pot of cotton at the diagonal of a cage with a length, width and height of 2m, 1m and 1.5m respectively, dilute diallyl disulfide or propyl disulfide with n-hexane to 10μg / μL, drip it on the filter paper, insert a toothpick through the filter paper into the soil of one pot of cotton, and insert the filter paper with the same volume of n-hexane dripped into the other pot of cotton. Release 20 winged cotton aphids from the middle of the cage, and observe the number of aphids on the two pots of cotton after 24 hours.

[0037] Test results, such as Figure 3 As shown, relative to n-hexane, diallyl disulfide (χ 2 =25.941, P<0.05) and propyl disulfide (χ 2 =3.959, P<0.05) had a significant repellent effect on cotton aphids.

[0038] Example 3: Verification of the repellent effect of diallyl disulfide and propyl disulfide on cotton aphids by half-leaf method

[0039] Test method: Place a piece of cotton leaf in a petri dish, dilute diallyl disulfide or propyl disulfide with n-hexane to 1 μg / μL, and drip 20 μL of diallyl disulfide or propyl disulfide on one side and 20 μL of n-hexane on the other side, using the main vein of the cotton leaf as the dividing line. Place 20 winged cotton aphids on the main vein of the cotton and allow them to choose freely. After 30 minutes, 1 hour, 2 hours, 4 hours, and 8 hours, the number of aphids on both sides of the leaf is recorded.

[0040] Test results: Figure 4 As shown, the cotton leaves dripped with diallyl disulfide were exposed to the air for 30 min (χ 2 =24.009,P<0.05), 1h(χ 2 =52.531,P<0.05), 2h(χ 2 =51.882,P<0.05), 4h(χ 2 =54.226, P<0.05) and 8h(χ 2 =42.667, P<0.05) had a significant repellent effect on cotton aphids. Figure 5 As shown, the cotton leaves with propyl disulfide were exposed to the sun for 30 min (χ 2 =37.902, P<0.05), 1h(χ 2 =60.466,P<0.05), 2h(χ 2 =47.207,P<0.05), 4h(χ 2 =40.333, P<0.05) and 8h(χ2 =34.776, P<0.05) had a significant repellent effect on cotton aphids.

[0041] Example 4: Repellent effect of different ratios of diallyl disulfide and propyl disulfide on cotton aphids

[0042] Experimental method: Place a piece of cotton leaf in a culture dish, dilute diallyl disulfide or propyl disulfide with n-hexane to 1 μg / μL, and drop the diluted diallyl disulfide and propyl disulfide into both sides of the main vein of the cotton leaf in a volume ratio of 1:0, 0:1, 1:1, 2:1, 1:2, 3:1, and 1:3, respectively. Place 20 winged cotton aphids on the main vein of the cotton and allow them to choose freely. Record the number of aphids on both sides of the leaf after 30 minutes, 1 hour, 2 hours, 4 hours, and 8 hours.

[0043] Test results: As shown in Table 1, all ratios have a repellent effect on cotton aphids. The repellent effect is more significant when the ratio of diallyl disulfide to propyl disulfide is 1:1 and 1:2, reaching 57.4% and 59.8%.

[0044] Table 1 Repellent effects of allyl disulfide and propyl disulfide in different ratios on cotton aphids

[0045]

[0046] The above test results show that the diallyl disulfide and propyl disulfide provided by the present invention as cotton aphid repellent components have good repellent effects. The present invention has important application value in the green control of cotton aphids and has broad economic, ecological and social benefits.

[0047] Although the present invention is verified by taking the prevention and control of cotton aphid damage as an example, it is easy to understand that the present invention can also be applied to the prevention and control of other damages of cotton aphid.

[0048] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A compound combination for repelling cotton aphids, characterized in that: In the compound combination, the effective components for repelling cotton aphids are diallyl disulfide and propyl disulfide.

2. The compound combination for repelling cotton aphid according to claim 1, characterized in that: The volume ratio of diallyl disulfide to propyl disulfide is 1:0.3 to 1:

2.

3. The compound combination for repelling cotton aphid according to claim 2, characterized in that: The volume ratio of diallyl disulfide to propyl disulfide is 1:1 to 1:

2.

4. A cotton aphid repellent, characterized in that A compound combination for repelling cotton aphid comprising any one of claims 1 to 3.

5. The cotton aphid repellent according to claim 4, characterized in that In the cotton aphid repellent, the effective component is a mixture of diallyl disulfide and propyl disulfide, and the volume ratio of diallyl disulfide to propyl disulfide is 1:0.3-1:

2.

6. The cotton aphid repellent according to claim 5, characterized in that In the cotton aphid repellent, the effective component is a mixture of diallyl disulfide and propyl disulfide, and the volume ratio of diallyl disulfide to propyl disulfide is 1:1-1:

2.

7. The cotton aphid repellent according to any one of claims 4 to 6, characterized in that: The cotton aphid repellent is a solution containing the effective components, and the solvent of the solution is n-hexane, ether or acetone.

8. The cotton aphid repellent according to claim 7, characterized in that The solvent of the solution is n-hexane.

9. The cotton aphid repellent according to claim 7, characterized in that In the solution, the usage ratio of diallyl disulfide and propyl disulfide to the solvent is 5-15 μg:1 μL.

10. The cotton aphid repellent according to claim 9, characterized in that The dosage ratio of diallyl disulfide and propyl disulfide to n-hexane in the solution is 10 μg:1 μL.

11. Use of a mixture of diallyl disulfide and propyl disulfide in preparing a cotton aphid repellent product.

12. The use according to claim 11, characterized in that The cotton aphid repellent product is a cotton aphid repellent or a cotton aphid repellent device.

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