A seed treatment agent composition and its application

Abstract

The present invention belongs to the technical field of pesticides, and particularly relates to a seed treatment agent composition and its application. The effective active ingredients of the composition are indoleacyl hydrazide and fluxapyroxad, and the weight ratio of indoleacyl hydrazide to fluxapyroxad is 20:1 - 1:20. This composition can improve the germination rate and germination potential of seeds, reduce the harm of diseases, ensure the healthy growth of crops, effectively reduce the per-acre usage amount of the seed treatment agent, improve the utilization rate of the agent, increase the germination rate of seeds, reduce the invasion of pathogens, and increase crop yields; when the composition is used for seed dressing, it enables precise application of pesticides, reduces the usage amount of pesticides, and is beneficial to environmental protection.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a seed treatment agent composition and application thereof. Background Art

[0002] With the rapid growth of the world's population and the increasing demand for food production, as well as the green transformation of global agriculture, the influence of seed coating on food production is increasing. Effective seed treatment can prevent the spread of seed-borne and soil-borne diseases, and can also improve the overall vitality and resistance of crops. Generally, coated seeds do not need to be applied with pesticides for nearly 45 days during the germination and seedling stages, and the amount of pesticides used is only about 1 / 50 of that applied in the field.

[0003] Seed treatment agents have many advantages such as high efficiency, long-lasting effect, economy and environmental protection. However, seed treatment agents currently have the following problems: ① The combination of agents is generally mainly for disease and insect prevention, and cannot take into account the growth and stress resistance of seeds and crops; ② There is a serious phenomenon of similarity among seed treatment products on the market, and the seed mixing effect is poor due to large-scale repeated use.

[0004] Chlorovindole hydrazide is a new compound developed by Jingbo Agrochemical in cooperation with Nankai University based on tetrahydrocarboline hydrazide compounds. It can activate the antioxidant system of plants and the salicylic acid pathway of plants, and has a certain preventive effect on diseases such as powdery mildew, ergot, and downy mildew. However, there have been no reports on the use of chlororovindole hydrazide as a seed treatment agent.

[0005] Fluoxathiapiprolin is a new piperidine fungicide developed by DuPont. Its mechanism of action is an inhibitor of oxysterol-binding proteins. It has a good preventive effect on late blight, downy mildew, root rot, blight and other diseases, and can also be used as a seed treatment agent. However, fluoxathiapiprolin has a single site of action and has a medium to high level of resistance risk. Summary of the invention

[0006] Based on the current technical problems, the present invention provides a seed treatment agent composition and application. The effective active ingredients of the seed treatment agent composition are chloroindole hydrazide and fluthiazolinone, which can significantly reduce the occurrence of crop diseases, improve the germination rate of seeds, reduce pathogenic bacteria invasion, and increase crop yields. The two active components of chloroindole hydrazide and fluthiazolinone are compounded to reduce the dosage of the effective ingredients and delay the occurrence of resistance.

[0007] It is known in the prior art that chloroindole hydrazide has the effect of preventing and controlling diseases. Through continuous exploration, the inventor has found that chloroindole hydrazide can not only enhance the disease resistance of crops, but also have a certain effect on regulating plant growth, improve the germination rate of seeds, ensure the healthy growth of crops, and have a certain yield increase effect; because chloroindole hydrazide has partial same structure with the plant growth regulator indoleacetic acid, it can promote root growth of seeds, promote cell differentiation and division, and make the crop roots developed; in order to effectively delay the generation of drug resistance and extend the life cycle of the medicament. In the present invention, chloroindole hydrazide and flutriafol with different action mechanisms are compounded and used. The combination of chloroindole hydrazide and flutriafol is used as a seed treatment agent. The seeds treated have fast emergence, developed roots, healthier plants, and less dosage of the drug, and have a good synergistic effect.

[0008] The specific technical solution of the present invention is as follows:

[0009] A seed treatment agent composition, the effective active ingredients of which are chloroindole hydrazide and flutriafol, and the weight ratio of chloroindole hydrazide to flutriafol is 20:1 - 1:20.

[0010] Preferably, the total weight of chloroindole hydrazide and flutriafol accounts for 5 - 70% of the total weight of the compound composition.

[0011] Preferably, the total weight of chloroindole hydrazide and flutriafol accounts for 10 - 50% of the total weight of the compound composition.

[0012] Preferably, the weight ratio of chloroindole hydrazide to flutriafol is 1 - 10:10 - 1.

[0013] Preferably, the weight ratio of chloroindole hydrazide to flutriafol is 1 - 5:5 - 1, and further preferably, the weight ratio of chloroindole hydrazide to flutriafol is 2:1.

[0014] Preferably, the dosage form of the composition is a suspension seed coating agent and a seed treatment dispersible powder.

[0015] On the basis of the above technical solution, the auxiliary agents included in the composition are as follows: dispersant, wetting agent, thickener, filler, antifreeze, defoamer, film-forming agent, warning color.

[0016] Preferably, the dispersant is one or a combination of any proportions of phosphate ester dispersants, carboxylate dispersants, naphthalene sulfonate dispersants, and lignin dispersants.

[0017] Preferably, the wetting agent is at least one of modified alkylphenol polyoxyethylene ethers, alkyl sulfonate salts, and Nekal.

[0018] Preferably, the thickener is at least one of xanthan gum and silica white.

[0019] Preferably, the antifreeze is glycerol or ethylene glycol.

[0020] Preferably, the film-forming agent is one or a combination of any proportions of polyvinyl alcohol, carboxymethyl cellulose, gum arabic, sodium alginate, etc.

[0021] Preferably, the warning color is one or more of rhodamine, rose red, and scarlet.

[0022] Preferably, the filler is one or a combination of any proportions of kaolin, light calcium carbonate, bentonite, diatomaceous earth, sodium-based bentonite, etc.

[0023] Preferably, the defoaming agent mainly includes silicone, etc.

[0024] Further on the basis of the above technical solutions, the inventor provides the following specific technical solutions for the corresponding dosage forms:

[0025] Suspension seed coating agent: A flowable, stable and uniform suspension liquid made by wet grinding of chlorindoleacyl hydrazide, flutriafol, and appropriate wetting agent, dispersant, film-forming agent, warning color, antifreeze, thickening agent, defoaming agent, and water.

[0026] Specific steps of operation: 1) First, mix deionized water, dispersant, wetting agent, film-forming agent, warning color, and antifreeze, then add chlorindoleacyl hydrazide, flutriafol technical drug, thickening agent, and defoaming agent, and stir evenly; 2) Add the mixed liquid in step 1) to a sand mill for grinding until the particle size D95 = 5um, and control the temperature below 30°C during the grinding process; finally, filter and mix to obtain the chlorindoleacyl hydrazide · flutriafol suspension seed coating agent;

[0027] The weight percentages of each component are as follows:

[0028] Chloroindole hydrazide 5％-50％ Flutriafol 5％-50％ Wetting agent 1％-3％ Dispersant 1％-8％ Film-forming agent 0.2％-6％ Thickener 0.05％-2％ Antifreeze 3％-8％ Defoamer 0.01％-0.1％ Warning color 0.1％-15％ Water Balance

[0029] Seed treatment dispersible powder composition, made by mixing and coarsely pulverizing, and air-flow pulverizing of chlorindoleacyl hydrazide, flutriafol, and appropriate wetting agent, dispersant, and filler;

[0030] The weight percentages of each component are as follows:

[0031] Chloroindole hydrazide 5％-70％ Flutriafol 5％-70％ Wetting agent 1％-20％ Dispersant 1％-20％ Film-forming agent 0.1-6％ Warning color 0.1％-15％ Filler Balance

[0032] Application of the above seed treatment agent composition in the technical field of crop seed treatment, and the crops are soybeans, potatoes, wheat, peanuts, peppers, tomatoes, watermelons, rice and other crops.

[0033] A method for using a seed treatment agent composition. When using the composition, add a small amount of water and dilute it evenly (it is sufficient to be able to evenly coat the seeds), then coat the seeds and let them dry before use. The usage amount of the composition is 1 - 1.5 g of active ingredient per 10 kg of seeds.

[0034] The seed treatment agent composition containing chlorindoleacyl hydrazide and flutriafol provided by the present invention can improve the germination rate and germination potential of seeds, reduce the harm of diseases, and ensure the healthy growth of crops; the composition of the present invention can effectively reduce the per-acre usage amount of the seed treatment agent, improve the utilization rate of the agent, increase the germination rate of seeds, reduce the invasion of germs, and increase crop yields; the use of this composition for coating seeds enables precise application of the medicine, reduces the usage amount of the medicine, and is beneficial to environmental protection; the present invention is a compound of two agents with different action mechanisms, namely chlorindoleacyl hydrazide and flutriafol, which can complement each other's advantages, effectively delay the problem of drug resistance caused by a single agent, is beneficial to extending the market life of the compound, and has great social significance. Detailed implementation manners

[0035] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0036] The experimental methods used in the following embodiments are all conventional methods unless otherwise specified.

[0037] The materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0038] DSC2006 used in the following embodiments is purchased from Beijing Hanmoke; RYD982 is purchased from Rongyida; GY-D07 is purchased from Guangyuan Yinuo; GY-D800 is purchased from Guangyuan Yinuo; D450 is purchased from AkzoNobel; 860P is purchased from Solvay; Sag 1522 is purchased from Momentive; other raw materials are all products that can be easily purchased on the market.

[0039] Example 1: 10% chlorindoleacyl hydrazide · flutriafol suspension seed coating agent (1:1)

[0040] Chlorindoleacyl hydrazide, 5%; flutriafol, 5%, dispersant DSC2006, 3%; wetting agent 860P, 1%; polyvinyl alcohol, 2%, rose bengal, 3%; defoaming agent Sag 1522, 0.3%; antifreeze glycerol, 4%; thickening agent xanthan gum, 0.2%; deionized water, 76.5%.

[0041] Example 2: 20% chlorindoleacyl hydrazide · flutriafol suspension seed coating agent (3:1)

[0042] Chloroindole hydrazide, 15%; Fluoxastrobin, 5%, Dispersant DSC2006, 4%; Wetting agent 860P, 1%; Polyvinyl alcohol, 3%, Rhodamine B, 5%; Defoamer Sag 1522, 0.3%; Antifreeze glycerol, 4%; Thickener xanthan gum, 0.15%; Deionized water, 62.55%.

[0043] Example 3: 30% Chloroindole hydrazide · Fluoxastrobin Suspension Seed Coating Agent (2:1)

[0044] Chloroindole hydrazide, 20%; Fluoxastrobin, 10%, Dispersant RYD982, 3%; GY-D07, 2%; Wetting agent 860P, 1%; Polyvinyl alcohol, 3%, Rhodamine B, 6%; Defoamer Sag 1522, 0.3%; Antifreeze glycerol, 4%; Thickener xanthan gum, 0.1%; Deionized water, 50.6%.

[0045] Example 4: 40% Chloroindole hydrazide · Fluoxastrobin Suspension Seed Coating Agent (1:9)

[0046] Chloroindole hydrazide, 4%; Fluoxastrobin, 36%, Dispersant GY-D07, 2%; DSC2006, 3%; Wetting agent 860P, 1%; Polyvinyl alcohol, 4%, Rhodamine B, 8%; Defoamer Sag 1522, 0.3%; Antifreeze glycerol, 4%; Thickener xanthan gum, 0.08%; Deionized water, 37.62%.

[0047] Example 5: 50% Chloroindole hydrazide · Fluoxastrobin Suspension Seed Coating Agent (2:3)

[0048] Chloroindole hydrazide, 20%; Fluoxastrobin, 30%, Dispersant GY-D07, 3%; DSC2006, 3%; Wetting agent 860P, 1%; Polyvinyl alcohol, 4%, Rhodamine B, 10%; Defoamer Sag 1522, 0.3%; Antifreeze glycerol, 3%; Thickener xanthan gum, 0.08%; Deionized water, 25.62%.

[0049] The above examples are operated according to the following steps: 1) First, mix deionized water, dispersant, wetting agent, film-forming agent, warning color, and antifreeze, then add the original drugs of chloroindole hydrazide and fluoxastrobin, thickener and defoamer, and stir evenly; 2) Add the mixed solution in step (1) to a sand mill for grinding until the particle size D95 = 5um, and control the temperature below 30°C during the grinding process; finally, filter and mix evenly to obtain the chloroindole hydrazide · fluoxastrobin suspension seed coating agent.

[0050] Example 6: 30% Chloroindole hydrazide · Fluoxastrobin Seed Treatment Dispersible Powder (2:1)

[0051] Chloroindole hydrazide, 20%; Fluoxastrobin, 10%, Dispersant GY-D800, 2%; D450, 2%; Nekal BX, 1%; Diatomite, 10%; Sodium carboxymethyl cellulose, 0.2%; Scarlet red, 3%; Kaolin, 51.8%.

[0052] Example 7: 40% Chloroindole hydrazide·Fluoxastrobin Seed Treatment Dispersible Powder (1:3)

[0053] Chloroindole hydrazide, 10%; Fluoxastrobin, 30%, Dispersant GY-D800, 3%; D450, 2%; Nekal BX, 1%; Organobentonite, 5%; Sodium carboxymethyl cellulose, 0.3%, Scarlet red, 4%; Kaolin, 44.7%.

[0054] Example 8: 50% Chloroindole hydrazide·Fluoxastrobin Seed Treatment Dispersible Powder (1:4)

[0055] Chloroindole hydrazide, 10%; Fluoxastrobin, 40%, Dispersant GY-D800, 3%; D450, 4%; Sodium dodecyl sulfate, 1%; Light calcium carbonate, 4%; Sodium carboxymethyl cellulose, 0.4%, Scarlet red, 5%; Kaolin, 32.6%.

[0056] Example 9: 60% Chloroindole hydrazide·Fluoxastrobin Seed Treatment Dispersible Powder (9:1)

[0057] Chloroindole hydrazide, 54%; Fluoxastrobin, 6%, Dispersant GY-D800, 4%; D450, 4%; Sodium dodecyl sulfate, 1%; Light calcium carbonate, 5%; Sodium carboxymethyl cellulose, 0.5%; Scarlet red, 6%; Kaolin, 19.5%.

[0058] Example 10: 70% Chloroindole hydrazide·Fluoxastrobin Seed Treatment Dispersible Powder (4:3)

[0059] Chloroindole hydrazide, 40%; Fluoxastrobin, 30%, Dispersant GY-D800, 4%; D450, 5%; Sodium dodecyl sulfate, 2%; Light calcium carbonate, 5%; Sodium carboxymethyl cellulose, 0.6%; Scarlet red, 7%; Kaolin, 6.4%.

[0060] The formulation of the above seed treatment dispersible powder examples is operated according to the following steps: 1) First, mix the raw materials of chloroindole hydrazide, fluoxastrobin, dispersant, wetting agent, film-forming agent, warning color and filler evenly; 2) Crush the mixed materials in step (1) by a jet mill until the passing rate of the material particles through a 75um sieve is ≥98%, and finally mix evenly to obtain different chloroindole hydrazide·fluoxastrobin seed treatment dispersible powders.

[0061] Comparative Example 1: 10% Chlorindoleacyl Hydrazide Suspension Seed Coating Agent

[0062] Chlorindoleacyl hydrazide, 10%; Dispersant DSC2006, 3%; Wetting agent 860P, 1%; Polyvinyl alcohol, 2%, Rhodamine, 3%; Defoaming agent Sag 1522, 0.3%; Antifreezing agent glycerol, 4%; Thickening agent xanthan gum, 0.2%; Deionized water, 76.5%.

[0063] Comparative Example 2: 10% Flutriafol Pyrifluquinazon Suspension Seed Coating Agent

[0064] Flutriafol pyrifluquinazon, 10%; Dispersant DSC2006, 3%; Wetting agent 860P, 1%; Polyvinyl alcohol, 2%, Rhodamine, 3%; Defoaming agent Sag 1522, 0.3%; Antifreezing agent glycerol, 4%; Thickening agent xanthan gum, 0.2%; Deionized water, 76.5%.

[0065] Comparative Example 3: 10% Chlorindoleacyl Hydrazide · Metalaxyl Suspension Seed Coating Agent (1:1)

[0066] Chlorindoleacyl hydrazide, 5%; Metalaxyl, 5%, Dispersant DSC2006, 3%; Wetting agent 860P, 1%; Polyvinyl alcohol, 2%, Rhodamine, 3%; Defoaming agent Sag 1522, 0.3%; Antifreezing agent glycerol, 4%; Thickening agent xanthan gum, 0.2%; Deionized water, 76.5%.

[0067] Comparative Example 4: 10% Flutriafol Pyrifluquinazon · Fludioxonil Suspension Seed Coating Agent (1:1)

[0068] Flutriafol pyrifluquinazon, 5%; Fludioxonil, 5%, Dispersant DSC2006, 3%; Wetting agent 860P, 1%; Polyvinyl alcohol, 2%, Rhodamine, 3%; Defoaming agent Sag 1522, 0.3%; Antifreezing agent glycerol, 4%; Thickening agent xanthan gum, 0.2%; Deionized water, 76.5%.

[0069] Biological Test

[0070] Wheat Seed Dressing Experiment

[0071] The efficacy test of the composition of the present invention for wheat seed dressing was carried out at an experimental base in Pangjia Town, Boxing County, Binzhou City, Shandong Province. The test soil had a weight ratio of soil to substrate of 2:1. After sowing, the seedling pots were placed in a plant cultivation room for cultivation (temperature: 22 ± 3°C, humidity: 55 ± 5%, light: 4000 lx for 14 hours during the day, 0 lx for 10 hours at night), and no other agents were used during the test period. Experimental design: There were 13 treatments, with 10 pots for each treatment. After seed dressing with the agent (first add a small amount of water to the seed treatment agent and mix the seeds evenly, then stir evenly and dilute and mix with the seeds again), dry them indoors. Before sowing, water 300 ml into each pot and sow 30 wheat seeds (90 mm double-color seedling pots) as shown in Table 1 below. The experimental agents were the samples of Examples 1 - 10, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4. Investigation method: A total of two investigations were carried out after sowing to investigate the germination rate of the seeds and to statistically analyze the germination potential and germination rate growth indicators of different agent treatments.

[0072] The calculations of germination potential and germination rate are shown in Formulas (1) and (2) respectively.

[0073] Germination potential (Gv) = M1 / M × 100% ………………………………… (1)

[0074] Germination rate (Gp) = M2 / M × 100% ………………………………… (2)

[0075] Among them, M1 is the number of normally germinated seeds within the germination potential days (5 days); M2 is the number of all normally germinated seeds (10 days); M is the number of tested seeds.

[0076] Table 1 Experimental design and results

[0077]

[0078]

[0079] As can be seen from Table 1, after seed dressing, for Examples 1 - 10, the germination potential of wheat seeds was better than that of the comparative examples, and the germination rate of wheat seeds in the examples was higher than that of the comparative examples. Among them, the germination test and germination rate of wheat seeds in Examples 3 and 6 were the highest.

[0080] Potato seed dressing experiment

[0081] The experiment was carried out in a plot with relatively severe early blight of potatoes in Dianzi Town, Boxing County, Shandong Province. The texture was loam, the irrigation water was groundwater, and the drip irrigation method was used. 667 m 2Apply 20 kg of diammonium phosphate and 10 kg of potassium sulfate as base fertilizers. Sow by means of mechanical furrowing and manual dibbling. Water by drip irrigation throughout the growth period. Do not use any other pesticides during the experiment. Experimental design: The experiment has 13 treatments. There are 100 potato tubers in total for each treatment. After seed dressing with pesticides (first add a small amount of water to the seed treatment agent and mix evenly with the seeds), dry them indoors. A total of 100 potato tubers in each treatment are used for the experiment. The experimental pesticides are the samples of Examples 1 - 10 and Comparative Examples 1 - 4. Investigation method: Randomly select samples in the experimental plots, investigate the incidence of early blight in the middle and late stages, record the number of diseased plants, and calculate the incidence rate. See Table 2 for details.

[0082] Table 2 Investigation on the incidence of early blight in potatoes under different treatments

[0083]

[0084] As can be seen from Table 2, after seed dressing treatment, compared with the control treatment with clear water, the incidence rate is the highest, exceeding 50%. After most of the Example treatments, the incidence rate of potatoes is less than 20%, which is better than that when the two pesticides are used alone and other seed treatment agents are used; among them, the incidence rates of Example 3 and Example 6 are relatively low, and the incidence rate of potatoes is less than 10%.

[0085] Soybean seed dressing experiment

[0086] The experiment is carried out in a greenhouse in Pangjia Town, Boxing County, Binzhou City, Shandong Province. The experimental soil is the field soil with serious root rot. The ratio of soil to substrate in the experimental soil is 1:1. Keep the soil moist during the experiment. The experimental temperature is 22 ± 3°C. Do not use any other pesticides during the experiment. Before sowing, dress the seeds with the designed dosage of pesticides (first add a small amount of water to the seed treatment agent, mix evenly, and dry for later use); Experimental design: The experiment adopts the randomized block method. The experiment has 13 treatments. There are 20 pots for each treatment. Sow 10 seeds in each pot. Use the treatment of seeds with clear water as the control. Take the field soil samples with serious root rot, mix them evenly and put them into flower pots of 30 cm * 20 cm. A total of 200 seeds are planted for each treatment. There are 20 pots for each treatment. Sow 10 seeds in each pot.

[0087] Determination of disease index and control effect: 25 days after emergence, randomly pull out some plants, investigate the disease conditions of roots and stems, record the total number of plants and the number of diseased plants, and calculate the incidence rate. See Table 3 for details.

[0088] Table 3 Disease index and control effect of each treatment on soybeans

[0089]

[0090] As can be seen from Table 3, after seed dressing treatment, compared with the control treated with clear water, all soybeans were diseased; the incidence rates of soybeans treated with the two pesticides alone were 50% and 46%; the incidence rate of soybeans after seed dressing with the combination of the two pesticides was less than 21%; the incidence rates of Comparative Example 3 and Comparative Example 4 were 36% and 31%; the incidence rate of soybeans after seed dressing was significantly lower than that of the two pesticides used alone and that of other seed dressing combinations. For the seeds treated in Example 3 and Example 6 of the composition of the present invention, the incidence rates of soybeans were 4% and 6% respectively.

[0091] In summary, the seed treatment agent composition containing indoleacyl hydrazide and fluxapyroxad provided by the present invention can not only improve the germination rate and germination potential of seeds, but also reduce the harm of plant diseases and ensure the healthy growth of crops; the seed dressing application of the indoleacyl hydrazide and fluxapyroxad seed treatment agent composition enables precise pesticide application, reduces the dosage of pesticides, and is beneficial to environmental protection; the compounding of two pesticides with different action mechanisms, indoleacyl hydrazide and fluxapyroxad, can complement each other's advantages and effectively delay the resistance problem caused by a single pesticide; it is beneficial to extend the market life of the compound and has great social significance.

Claims

1. A seed treatment agent composition, characterized in that, its active ingredients are indoleacyl hydrazide and fluxapyroxad, and the weight ratio of indoleacyl hydrazide to fluxapyroxad is 1-10:10-1; the total weight of indoleacyl hydrazide and fluxapyroxad accounts for 5-70% of the total weight of the compound composition; the dosage forms that can be prepared from the composition are suspension seed coating agents and seed treatment dispersible powders.

2. The seed treatment agent composition according to claim 1, characterized in that, the total weight of indoleacyl hydrazide and fluxapyroxad accounts for 10-50% of the total weight of the compound composition.

3. The seed treatment agent composition according to claim 1, characterized in that, the weight ratio of indoleacyl hydrazide to fluxapyroxad is 1-5:5-1.

4. The seed treatment agent composition according to claim 1, characterized in that, the weight ratio of indoleacyl hydrazide to fluxapyroxad is 2:

1.

5. Use of the seed treatment agent composition according to any one of claims 1-4 in the technical field of crop seed treatment.

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