Method for biodegrading antibiotics from livestock and poultry breeding waste
The treatment of livestock and poultry breeding waste through microbial degradation methods, and the preparation of microspheres and flocculants using mixed bacterial strains has solved the problems of large investment in equipment and complex processes in the existing technology, and achieved efficient degradation of antibiotics and passivation of heavy metals, and prepared high-quality liquid fertilizers to reduce environmental pollution and health risks.
Patent Information
- Application Number
- CN202211472128.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-11-23
- Publication Date
- 2025-09-02
- Estimated Expiration
- 2042-11-23
AI Technical Summary
In the prior art, when dealing with heavy metals and antibiotics in livestock and poultry farming waste, the equipment investment is large, the process is complex, and the energy consumption is high, making it difficult to achieve large-scale treatment. In addition, organic fertilizers with excessive heavy metals and antibiotic content will cause land and water pollution, which will cause harm to human health through the accumulation of food chain.
Microbial degradation method is used to prepare microspheres and bioflocculants by mixing different strains, treat antibiotics in livestock and poultry manure and wastewater, and passivate heavy metals, and use the synergistic effects of strains and fermentation products to reduce harmful components, and prepare high-quality liquid fertilizers.
Effectively degrade antibiotics, passivate heavy metals, reduce plant absorption, reduce toxic and harmful components, prepare high-quality liquid fertilizers, reduce odor and poisoning, promote crop growth, and realize the reuse of livestock and poultry waste.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of livestock and poultry breeding waste treatment, and particularly relates to a method for biodegrading antibiotics in livestock and poultry breeding waste. Background Art
[0002] With the rapid development of the breeding industry, the amount of livestock and poultry breeding waste has increased sharply. The discharge of livestock and poultry manure can reach about 2 times the total discharge of industrial solid waste in the same year. At the same time, a large amount of wastewater is also generated during the livestock and poultry breeding process. Taking the pig farming industry as an example, according to the "Pollutant Emission Standard for Livestock and Poultry Breeding Industry (GB18596-2001)", if the maximum allowable discharge volume in winter (1.2m 3 Calculated at 100 pigs / day, pig farms nationwide discharge 2.04 billion tons of wastewater annually. Livestock and poultry waste, consisting of feces and wastewater, has become a significant source of pollution in rural areas. Its discharge volume is large, and its organic matter concentration is high. It contains large amounts of ammonia, nitrogen, phosphorus, and pathogenic microorganisms, and often contains excessive levels of heavy metals and antibiotics. If not effectively treated, it will cause significant pollution to the surrounding ecological environment and the living environment of residents.
[0003] Although existing research has found that composting livestock and poultry waste can produce organic fertilizer suitable for plant growth, the modern livestock and poultry industry, in an effort to improve economic efficiency, generally feeds livestock and poultry feeds fortified with heavy metals to promote growth and uses antibiotics to prevent and treat disease. As a result, only a small portion of the heavy metals and antibiotics ingested by livestock and poultry are absorbed and utilized, with the remainder excreted through feces and urine. Organic fertilizers made from livestock and poultry waste containing excessive levels of heavy metals and antibiotics can easily lead to excessive levels of heavy metals in soil and water. These heavy metals are then excessively absorbed by crops and ultimately accumulated in animals and humans through the food chain, posing health risks. At present, the treatment methods for heavy metals and antibiotics in livestock and poultry breeding waste mainly use physical and chemical treatment methods to passivate heavy metals in pig farm manure and transform them into forms that are more difficult for plants to absorb. These methods include flotation, low-temperature cracking, bioleaching, acidification and ultrasound; at the same time, chlorination, advanced oxidation, electrochemical treatment, adsorption, thin film, ultrasonic cavitation effect and other methods are used to remove antibiotics. However, these technologies require large investments in equipment, complex processes, and high energy consumption, and are not yet suitable for large-scale treatment. Summary of the Invention
[0004] In view of the shortcomings of the treatment methods of heavy metals and antibiotics in livestock and poultry breeding waste, such as large equipment investment, complex process and high energy consumption, the present invention discloses a method for biodegrading antibiotics in livestock and poultry breeding waste. The method uses microbial degradation to treat antibiotics in livestock and poultry breeding waste, adsorb and passivate heavy metals, reduce toxic and harmful components, and reduce plant absorption. At the same time, it plays a role in nitrogen fixation, phosphorus dissolution and potassium dissolution, which is conducive to the subsequent reuse of livestock and poultry breeding waste.
[0005] The present invention is achieved by adopting the following technical solutions:
[0006] A method for biodegrading antibiotics in livestock and poultry breeding waste, comprising the following steps:
[0007] (1) A mixture of round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus mucilaginosus, and lactic acid bacteria was dissolved in water, and then glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate were added and stirred to obtain bacterial agent A; N-isopropylacrylamide, chitosan, and ethylene glycol dimethacrylate were mixed and dissolved in water, and then ultrasonically dispersed for 5 to 10 minutes and stirred and heated to 50 to 60°C, and then ammonium persulfate was added and reacted at a constant temperature for 3 to 5 hours, and then cooled to room temperature, and then bacterial agent A was added and mixed at a speed of 300 to 500 r / min for 1 to 2 hours, and then filtered and dried to obtain microspheres containing microorganisms;
[0008] (2) Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus and Pseudomonas aeruginosa are mixed and added to an agar medium for cultivation to obtain a bacterial agent B; the mass ratio of the Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus, Pseudomonas aeruginosa and the agar medium is (5-8):(3-5):(1-2):(1-2):(5-8):100;
[0009] (3) Stirring and mixing livestock and poultry manure and livestock and poultry breeding wastewater, then adding the microspheres obtained in step (1) and the bacterial agent B obtained in step (2) and mixing them evenly, and then heating to 30-40° C. and treating at a constant temperature for 7-10 days to obtain a mixture A; the mass ratio of the livestock and poultry manure, livestock and poultry breeding wastewater, microspheres and bacterial agent B is 1: (3-5): (0.05-0.1): (0.05-1);
[0010] (4) After mixing zeolite, vinylsilane and ethanol, the mixture is ground at 40-50°C for 5-10 minutes to obtain a modified zeolite slurry, acrylic acid is added to the modified zeolite slurry, and the mixture is stirred and reacted at 40-50°C for 1-2 hours. After cooling naturally to room temperature, the microbial agent C is added and mixed evenly to obtain a bioflocculant; the mass ratio of the zeolite, vinylsilane and ethanol is 10:(0.3-0.5):(5-10); the mass ratio of the acrylic acid, microbial agent C and modified zeolite slurry is (2-5):(1-2):10; the microbial agent C is obtained by mixing yeast and rhodococcus in a mass ratio of 10:(1-2);
[0011] (5) Add the bioflocculant obtained in step (4) to the mixture A obtained in step (3), stir and react for 3 to 5 hours, then let it stand for 30 to 60 minutes and filter to obtain a mixture B free of antibiotics and heavy metals; the mass ratio of the mixture A to the bioflocculant is 10:(0.5 to 1).
[0012] The present invention uses microorganisms to degrade antibiotics in livestock and poultry manure and livestock and poultry breeding wastewater, while passivating heavy metals, and using microbial flocculation to remove the passivated heavy metals and various impurities, reducing toxic and harmful components and reducing plant absorption. In addition, the microorganisms also play a role in nitrogen fixation, phosphorus solubilization, and potassium solubilization, which is conducive to the subsequent reuse of livestock and poultry breeding waste. Among them, the present invention selects different types and different characteristics of bacterial strains for compounding based on the synergistic effect and antagonism of each strain, as well as the induction and inhibition of the metabolites produced by fermentation on the strains, maximizing the synergistic effect of each strain and reducing the antagonistic effect. In addition, N-isopropylacrylamide, chitosan, and ethylene glycol dimethacrylate are cross-linked under the action of ammonium persulfate initiator and a certain amount of bacterial strains are encapsulated to obtain microspheres containing microorganisms. The microspheres can slowly release the bacterial agent during the treatment process, further avoiding the antagonism between some bacterial strains in the early stage. At the same time, the metabolites of the early microbial fermentation are used to induce the later released bacterial strains, thereby improving the bacterial strain activity, thereby achieving better effect. The present invention also utilizes yeast and Rhodococcus to prepare a biological flocculant. At the same time, zeolite, vinylsilane and ethanol are mixed and then physically ground and modified. After the zeolite surface is modified by vinylsilane, acrylic acid is added to cross-link the modified zeolite to obtain a microbial carrier. The microbial carrier not only has better microbial adsorption performance, but also has good hydrophobicity. It can not only adsorb and fix microorganisms, but also improve the stability and activity of the microbial agent, and promote its flocculation with passivated heavy metals, thereby improving the flocculation effect of the microbial flocculant.
[0013] Furthermore, the mass ratio of the round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus mucilaginosus, lactic acid bacteria, glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, magnesium sulfate and water in step (1) is (3-5): (1-2): (1-2): (1-3): (5-8): (10-20): (0.5-1): (0.5-1): (0.2-0.5): 100. By controlling the dosage of different bacterial species, the synergistic effect between the bacterial species is improved, the nitrogen fixation, phosphorus solubilization and potassium solubilization of the microorganisms are improved, and at the same time, the components such as glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate and magnesium sulfate are quantitatively added to promote bacterial reproduction and improve bacterial activity.
[0014] Furthermore, the drying in step (1) is carried out at 40-50°C for 3-5 hours. The drying temperature and drying time are controlled to prevent the microorganisms in the microspheres from being exposed to excessively high temperatures for a long time and losing their vitality.
[0015] Furthermore, the livestock and poultry manure and livestock and poultry breeding wastewater described in step (3) are stirred and mixed, and then sterilized at 120-150° C., and then the microspheres and bacterial agent B are added for treatment. Treatment at 120-150° C. can effectively kill pathogens in livestock and poultry manure and livestock and poultry breeding wastewater.
[0016] Furthermore, in step (5), the bioflocculant obtained in step (4) is added to the mixture A obtained in step (3) and reacted for 3 to 5 hours under stirring conditions at a speed of 50 to 100 r / min. By controlling the stirring speed, on the one hand, the dispersion of the bioflocculant in the mixture A is promoted, and on the other hand, the binding and precipitation of the bioflocculant with the passivated heavy metals are enhanced, thereby improving the flocculation effect.
[0017] The application of the above-mentioned method for biodegrading antibiotics from livestock and poultry breeding waste is to use the obtained mixture B to prepare liquid fertilizer. Specifically, the mixture B, bagasse, water and fermentation agent are mixed and fermented for 20 to 30 days to obtain liquid fertilizer; the mass ratio of the mixture B, bagasse, water and composite fermentation agent is (10 to 15): (20 to 30): 50: (1 to 5).
[0018] Furthermore, the leavening agent comprises the following components in parts by weight: 10-15 parts of yeast, 10-15 parts of lactic acid bacteria, 3-5 parts of cellulase, and 3-5 parts of hemicellulase.
[0019] Compared with the existing technology, this technical solution has the following beneficial effects:
[0020] 1. The present invention compounds Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus and Pseudomonas, and utilizes the synergistic effect among the bacterial species to degrade antibiotics in livestock and poultry manure and livestock and poultry breeding wastewater, and passivate heavy metals. At the same time, the round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidal, and lactic acid bacteria are compounded and made into microspheres. By slowly releasing the bacterial agent in the middle and late stages of the fermentation process, the microspheres play the role of nitrogen fixation, phosphorus and potassium solubilization, and adsorb and convert harmful substances such as ammonia nitrogen, hydrogen sulfide and skatole, thereby reducing odor and toxicity, thereby facilitating the subsequent reuse of livestock and poultry breeding waste.
[0021] 2. The present invention uses microbial methods to treat livestock and poultry manure and livestock and poultry breeding wastewater, and then ferments them with bagasse, water, and a fermentation agent to prepare high-quality organic liquid fertilizer. The organic fertilizer can be widely applied to various crops, effectively promote crop growth, and increase crop yields. Moreover, the composite bacterial agent used can in situ convert harmful substances such as ammonia nitrogen, hydrogen sulfide, and skatole in livestock and poultry breeding manure, reduce odor and toxicity, and have no secondary pollutant emissions, thereby reducing the emission of harmful gases during the storage and transportation of biological organic fertilizer raw materials. DETAILED DESCRIPTION
[0022] The present invention is further illustrated by the following examples, which are not intended to limit the present invention. Specific experimental conditions and methods not specified in the following examples are conventional methods well known to those skilled in the art.
[0023] Example 1:
[0024] A method for biodegrading antibiotics in livestock and poultry breeding waste, comprising the following steps:
[0025] (1) A mixture of round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, and lactic acid bacteria was dissolved in water, and then glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate were added and stirred to obtain bacterial agent A; N-isopropylacrylamide, chitosan, and ethylene glycol dimethacrylate were mixed and dissolved in water, and then ultrasonically dispersed for 8 minutes, stirred and heated to 55°C, and then ammonium persulfate was added and reacted at a constant temperature for 4 hours, and then cooled to room temperature, and then bacterial agent A was added and mixed at a stirring speed of 400 r / min for 1.5 hours, and then filtered and dried at 45°C for 4 hours to obtain microspheres containing microorganisms; the mass ratio of the round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, lactic acid bacteria, glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, magnesium sulfate, and water was 4:1.5:1.5:2:6:15:0.8:0.8:0.3:100;
[0026] (2) Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus and Pseudomonas aeruginosa are mixed and added to an agar medium for cultivation to obtain a bacterial agent B; the mass ratio of the Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus, Pseudomonas aeruginosa and the agar medium is 6:4:1.5:1.5:6:100;
[0027] (3) The livestock and poultry manure and livestock and poultry breeding wastewater are stirred and mixed and sterilized at 130°C, and then the microspheres obtained in step (1) and the microbial agent B obtained in step (2) are added and mixed evenly, and then heated to 35°C and kept at a constant temperature for 8 days to obtain a mixture A; the mass ratio of the livestock and poultry manure, livestock and poultry breeding wastewater, microspheres and microbial agent B is 1:4:0.08:0.06;
[0028] (4) After mixing zeolite, vinylsilane and ethanol, the mixture was ground at 45°C for 6 minutes to obtain a modified zeolite slurry, acrylic acid was added to the modified zeolite slurry, and the mixture was stirred and reacted at 45°C for 1.5 hours. After cooling naturally to room temperature, the microbial agent C was added and mixed evenly to obtain a bioflocculant; the mass ratio of the zeolite, vinylsilane and ethanol was 10:0.3:6; the mass ratio of the acrylic acid, microbial agent C and modified zeolite slurry was 3:1.5:10; the microbial agent C was obtained by mixing yeast and Rhodococcus in a mass ratio of 10:1.5;
[0029] (5) The bioflocculant obtained in step (4) was added to the mixture A obtained in step (3) and reacted for 3 h under stirring conditions at a speed of 80 r / min, and then allowed to stand for 45 min and filtered to obtain a mixture B free of antibiotics and heavy metals; the mass ratio of the mixture A to the bioflocculant was 10:0.5.
[0030] The mixture B obtained in this example was used to prepare liquid fertilizer. Specifically, the mixture B, bagasse, water and fermentation agent were mixed and fermented for 20 days to obtain liquid fertilizer; the mass ratio of the mixture B, bagasse, water and composite fermentation agent was 12:25:50:3; the fermentation agent included the following components in parts by weight: 12 parts of yeast, 13 parts of lactic acid bacteria, 3 parts of cellulase, and 4 parts of hemicellulase.
[0031] Example 2:
[0032] A method for biodegrading antibiotics in livestock and poultry breeding waste, comprising the following steps:
[0033] (1) A mixture of round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, and lactic acid bacteria was dissolved in water, and then glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate were added and stirred to obtain bacterial agent A; N-isopropylacrylamide, chitosan, and ethylene glycol dimethacrylate were mixed and dissolved in water, and then ultrasonically dispersed for 5 minutes and stirred and heated to 50°C, and then ammonium persulfate was added and reacted at a constant temperature for 3 hours, and then cooled to room temperature, and then bacterial agent A was added and mixed at a stirring speed of 300 r / min for 1 hour, and then filtered and dried at 40°C for 3 hours to obtain microspheres containing microorganisms; the mass ratio of the round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, lactic acid bacteria, glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, magnesium sulfate, and water was 3:1:1:1:5:10:0.5:0.5:0.2:100;
[0034] (2) Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus and Pseudomonas aeruginosa are mixed and added to an agar medium for cultivation to obtain a bacterial agent B; the mass ratio of the Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus, Pseudomonas aeruginosa and the agar medium is 5:3:1:1:5:100;
[0035] (3) The livestock and poultry manure and livestock and poultry breeding wastewater are stirred and mixed and sterilized at 120°C, and then the microspheres obtained in step (1) and the microbial agent B obtained in step (2) are added and mixed evenly, and then heated to 30°C and kept at a constant temperature for 7 days to obtain a mixture A; the mass ratio of the livestock and poultry manure, livestock and poultry breeding wastewater, microspheres and microbial agent B is 1:3:0.05:0.05;
[0036] (4) After mixing zeolite, vinylsilane and ethanol, the mixture was ground at 40°C for 5 minutes to obtain a modified zeolite slurry, acrylic acid was added to the modified zeolite slurry, and the mixture was stirred and reacted at 40°C for 1 hour. After cooling naturally to room temperature, the microbial agent C was added and mixed evenly to obtain a bioflocculant; the mass ratio of the zeolite, vinylsilane and ethanol was 10:0.4:5; the mass ratio of the acrylic acid, microbial agent C and modified zeolite slurry was 2:1:10; the microbial agent C was obtained by mixing yeast and Rhodococcus at a mass ratio of 10:1;
[0037] (5) The bioflocculant obtained in step (4) was added to the mixture A obtained in step (3) and reacted for 4 h under stirring conditions at a speed of 50 r / min, and then allowed to stand for 30 min and filtered to obtain a mixture B free of antibiotics and heavy metals; the mass ratio of the mixture A to the bioflocculant was 10:0.8.
[0038] The mixture B obtained in this example was used to prepare liquid fertilizer. Specifically, the mixture B, bagasse, water and fermentation agent were mixed and fermented for 25 days to obtain liquid fertilizer; the mass ratio of the mixture B, bagasse, water and composite fermentation agent was 10:20:50:1; the fermentation agent included the following components in parts by weight: 10 parts of yeast, 10 parts of lactic acid bacteria, 4 parts of cellulase, and 3 parts of hemicellulase.
[0039] Example 3:
[0040] A method for biodegrading antibiotics in livestock and poultry breeding waste, comprising the following steps:
[0041] (1) A mixture of round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, and lactic acid bacteria was dissolved in water, and then glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate were added and stirred to obtain bacterial agent A; N-isopropylacrylamide, chitosan, and ethylene glycol dimethacrylate were mixed and dissolved in water, and then ultrasonically dispersed for 10 minutes, stirred and heated to 60°C, and then ammonium persulfate was added and reacted at a constant temperature for 5 hours, and then cooled to room temperature, and then bacterial agent A was added and mixed at a stirring speed of 500 r / min for 2 hours, and then filtered and dried at 50°C for 5 hours to obtain microspheres containing microorganisms; the mass ratio of the round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, lactic acid bacteria, glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, magnesium sulfate, and water was 5:2:2:3:8:20:1:1:0.5:100;
[0042] (2) Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus and Pseudomonas aeruginosa are mixed and added to an agar medium for cultivation to obtain a bacterial agent B; the mass ratio of the Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus, Pseudomonas aeruginosa and the agar medium is 8:5:2:2:8:100;
[0043] (3) The livestock and poultry manure and livestock and poultry breeding wastewater are stirred and mixed and sterilized at 150°C, and then the microspheres obtained in step (1) and the microbial agent B obtained in step (2) are added and mixed evenly, and then heated to 40°C and kept at a constant temperature for 10 days to obtain a mixture A; the mass ratio of the livestock and poultry manure, livestock and poultry breeding wastewater, microspheres and microbial agent B is 1:5:0.1:0.1;
[0044] (4) After mixing zeolite, vinylsilane and ethanol, the mixture was ground at 50°C for 10 minutes to obtain a modified zeolite slurry, acrylic acid was added to the modified zeolite slurry, and the mixture was stirred and reacted at 50°C for 2 hours. After cooling naturally to room temperature, the microbial agent C was added and mixed evenly to obtain a bioflocculant; the mass ratio of the zeolite, vinylsilane and ethanol was 10:0.5:10; the mass ratio of the acrylic acid, microbial agent C and modified zeolite slurry was 5:2:10; the microbial agent C was obtained by mixing yeast and Rhodococcus in a mass ratio of 10:2;
[0045] (5) The bioflocculant obtained in step (4) was added to the mixture A obtained in step (3) and reacted for 5 h under stirring conditions at a speed of 100 r / min, and then allowed to stand for 60 min and filtered to obtain a mixture B free of antibiotics and heavy metals; the mass ratio of the mixture A to the bioflocculant was 10:1.
[0046] The mixture B obtained in this example is used to prepare liquid fertilizer. Specifically, the mixture B, bagasse, water and fermentation agent are mixed and fermented for 30 days to obtain liquid fertilizer; the mass ratio of the mixture B, bagasse, water and composite fermentation agent is 15:30:50:5; the fermentation agent includes the following components in parts by weight: 15 parts of yeast, 15 parts of lactic acid bacteria, 5 parts of cellulase, and 5 parts of hemicellulase.
[0047] Example 4:
[0048] A method for biodegrading antibiotics in livestock and poultry breeding waste, comprising the following steps:
[0049] (1) A mixture of round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, and lactic acid bacteria was dissolved in water, and then glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate were added and stirred to obtain bacterial agent A; N-isopropylacrylamide, chitosan, and ethylene glycol dimethacrylate were mixed and dissolved in water, and then ultrasonically dispersed for 8 minutes, stirred and heated to 52°C, and then ammonium persulfate was added and reacted at a constant temperature for 4 hours, and then cooled to room temperature, and then bacterial agent A was added and mixed at a stirring speed of 400 r / min for 1.5 hours, and then filtered and dried at 42°C for 4 hours to obtain microspheres containing microorganisms; the mass ratio of the round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, lactic acid bacteria, glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, magnesium sulfate, and water was 4:1.2:1.2:1:7:12:0.6:0.8:0.4:100;
[0050] (2) Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus and Pseudomonas aeruginosa are mixed and added to an agar medium for cultivation to obtain a bacterial agent B; the mass ratio of the Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus, Pseudomonas aeruginosa and the agar medium is 7:4:1.2:1.4:7:100;
[0051] (3) The livestock and poultry manure and livestock and poultry breeding wastewater are stirred and mixed and sterilized at 140°C, and then the microspheres obtained in step (1) and the microbial agent B obtained in step (2) are added and mixed evenly, and then heated to 35°C and kept at a constant temperature for 9 days to obtain a mixture A; the mass ratio of the livestock and poultry manure, livestock and poultry breeding wastewater, microspheres and microbial agent B is 1:4:0.08:0.05;
[0052] (4) After mixing zeolite, vinylsilane and ethanol, the mixture was ground at 45°C for 6 minutes to obtain a modified zeolite slurry, acrylic acid was added to the modified zeolite slurry, and the mixture was stirred and reacted at 45°C for 1.5 hours. After cooling naturally to room temperature, the microbial agent C was added and mixed evenly to obtain a bioflocculant; the mass ratio of the zeolite, vinylsilane and ethanol was 10:0.35:6; the mass ratio of the acrylic acid, microbial agent C and modified zeolite slurry was 3:1.5:10; the microbial agent C was obtained by mixing yeast and Rhodococcus at a mass ratio of 10:1.5;
[0053] (5) The bioflocculant obtained in step (4) was added to the mixture A obtained in step (3) and reacted for 3 h under stirring conditions at a speed of 60 r / min, and then allowed to stand for 40 min and filtered to obtain a mixture B free of antibiotics and heavy metals; the mass ratio of the mixture A to the bioflocculant was 10:0.7.
[0054] The mixture B obtained in this example was used to prepare liquid fertilizer. Specifically, the mixture B, bagasse, water and fermentation agent were mixed and fermented for 22 days to obtain liquid fertilizer; the mass ratio of the mixture B, bagasse, water and composite fermentation agent was 13:22:50:4; the fermentation agent included the following components in parts by weight: 13 parts of yeast, 14 parts of lactic acid bacteria, 3 parts of cellulase, and 5 parts of hemicellulase.
[0055] Example 5:
[0056] A method for biodegrading antibiotics in livestock and poultry breeding waste, comprising the following steps:
[0057] (1) A mixture of round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, and lactic acid bacteria was dissolved in water, and then glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate were added and stirred to obtain bacterial agent A; N-isopropylacrylamide, chitosan, and ethylene glycol dimethacrylate were mixed and dissolved in water, and then ultrasonically dispersed for 5 minutes and stirred and heated to 54°C, and then ammonium persulfate was added and reacted at a constant temperature for 3 hours, and then cooled to room temperature, and then bacterial agent A was added and mixed at a stirring speed of 350 r / min for 1 hour, and then filtered and dried at 48°C for 3 hours to obtain microspheres containing microorganisms; the mass ratio of the round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, lactic acid bacteria, glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, magnesium sulfate, and water was 3:1.4:1:1:5:18:0.5:0.5:0.2:100;
[0058] (2) Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus and Pseudomonas aeruginosa are mixed and added to an agar medium for cultivation to obtain a bacterial agent B; the mass ratio of the Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus, Pseudomonas aeruginosa and the agar medium is 5:3:1.4:1.2:5:100;
[0059] (3) The livestock and poultry manure and livestock and poultry breeding wastewater are stirred and mixed and sterilized at 135°C, and then the microspheres obtained in step (1) and the microbial agent B obtained in step (2) are added and mixed evenly, and then heated to 40°C and kept at a constant temperature for 7 days to obtain a mixture A; the mass ratio of the livestock and poultry manure, livestock and poultry breeding wastewater, microspheres and microbial agent B is 1:3:0.07:0.06;
[0060] (4) After mixing zeolite, vinylsilane and ethanol, the mixture was ground at 40°C for 8 minutes to obtain a modified zeolite slurry, acrylic acid was added to the modified zeolite slurry, and the mixture was stirred and reacted at 40°C for 1 hour. After cooling naturally to room temperature, the microbial agent C was added and mixed evenly to obtain a bioflocculant; the mass ratio of the zeolite, vinylsilane and ethanol was 10:0.45:8; the mass ratio of the acrylic acid, microbial agent C and modified zeolite slurry was 2:1:10; the microbial agent C was obtained by mixing yeast and Rhodococcus at a mass ratio of 10:1;
[0061] (5) The bioflocculant obtained in step (4) was added to the mixture A obtained in step (3) and reacted for 4 h under stirring conditions at a speed of 70 r / min, and then allowed to stand for 50 min and filtered to obtain a mixture B free of antibiotics and heavy metals; the mass ratio of the mixture A to the bioflocculant was 10:0.6.
[0062] The mixture B obtained in this example is used to prepare liquid fertilizer. Specifically, the mixture B, bagasse, water and fermentation agent are mixed and fermented for 28 days to obtain liquid fertilizer; the mass ratio of the mixture B, bagasse, water and composite fermentation agent is 14:28:50:2; the fermentation agent includes the following components in parts by weight: 14 parts of yeast, 10 parts of lactic acid bacteria, 4 parts of cellulase, and 3 parts of hemicellulase.
[0063] Example 6:
[0064] A method for biodegrading antibiotics in livestock and poultry breeding waste, comprising the following steps:
[0065] (1) A mixture of round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, and lactic acid bacteria was dissolved in water, and then glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate were added and stirred to obtain bacterial agent A; N-isopropylacrylamide, chitosan, and ethylene glycol dimethacrylate were mixed and dissolved in water, and then ultrasonically dispersed for 10 minutes and stirred and heated to 58°C, and then ammonium persulfate was added and reacted at a constant temperature for 5 hours, and then cooled to room temperature, and then bacterial agent A was added and mixed at a stirring speed of 450 r / min for 2 hours, and then filtered and dried at 50°C for 5 hours to obtain microspheres containing microorganisms; the mass ratio of the round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, lactic acid bacteria, glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, magnesium sulfate, and water was 5:1:2:3:8:20:1:1:0.5:100;
[0066] (2) Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus and Pseudomonas aeruginosa are mixed and added to an agar medium for cultivation to obtain a bacterial agent B; the mass ratio of the Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus, Pseudomonas aeruginosa and the agar medium is 8:5:2:1:8:100;
[0067] (3) The livestock and poultry manure and livestock and poultry breeding wastewater are stirred and mixed and sterilized at 125°C, and then the microspheres obtained in step (1) and the microbial agent B obtained in step (2) are added and mixed evenly, and then heated to 30°C and kept at a constant temperature for 10 days to obtain a mixture A; the mass ratio of the livestock and poultry manure, livestock and poultry breeding wastewater, microspheres and microbial agent B is 1:5:0.1:0.05;
[0068] (4) After mixing zeolite, vinylsilane and ethanol, the mixture was ground at 50°C for 9 minutes to obtain a modified zeolite slurry, acrylic acid was added to the modified zeolite slurry, and the mixture was stirred and reacted at 50°C for 2 hours. After cooling naturally to room temperature, the microbial agent C was added and mixed evenly to obtain a bioflocculant; the mass ratio of the zeolite, vinylsilane and ethanol was 10:0.5:9; the mass ratio of the acrylic acid, microbial agent C and modified zeolite slurry was 5:2:10; the microbial agent C was obtained by mixing yeast and Rhodococcus in a mass ratio of 10:2;
[0069] (5) The bioflocculant obtained in step (4) was added to the mixture A obtained in step (3) and reacted for 5 h under stirring conditions at a speed of 100 r / min, and then allowed to stand for 55 min and filtered to obtain a mixture B free of antibiotics and heavy metals; the mass ratio of the mixture A to the bioflocculant was 10:1.
[0070] The mixture B obtained in this example was used to prepare liquid fertilizer. Specifically, the mixture B, bagasse, water and fermentation agent were mixed and fermented for 30 days to obtain liquid fertilizer; the mass ratio of the mixture B, bagasse, water and composite fermentation agent was 10:30:50:4; the fermentation agent included the following components in parts by weight: 15 parts of yeast, 15 parts of lactic acid bacteria, 5 parts of cellulase, and 4 parts of hemicellulase.
[0071] Comparative Example 1:
[0072] The method for biodegrading antibiotics from livestock and poultry waste described in this comparative example is different from the method described in Example 1 only in that, in step (1), no microspheres are prepared, but a mixture of round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, and lactic acid bacteria is dissolved in water, and then glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate are added and stirred to obtain bacterial agent A; the round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus colloidus, lactic acid bacteria, glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate are mixed and stirred to obtain bacterial agent A; The mass ratio of potassium monohydrogen, magnesium sulfate and water is 4:1.5:1.5:2:6:15:0.8:0.8:0.3:100; in step (3), livestock and poultry manure and livestock and poultry breeding wastewater are stirred and mixed and sterilized at 130°C, then the microspheres obtained in step (1) and the microbial agent B obtained in step (2) are added and mixed evenly, and then heated to 35°C and kept at a constant temperature for 8 days to obtain a mixture A; the mass ratio of livestock and poultry manure, livestock and poultry breeding wastewater, microbial agent A and microbial agent B is 1:4:0.08:0.06.
[0073] Comparative Example 2:
[0074] The method for biodegrading antibiotics from livestock and poultry waste described in this comparative example is different from the method described in Example 1 only in that, in step (1), a mixture of round-brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus cereus, Pseudomonas, and lactic acid bacteria is dissolved in water, and then glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate are added and stirred evenly to obtain bacterial agent A; the mass ratio of the round-brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus cereus, Pseudomonas, lactic acid bacteria, glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, magnesium sulfate, and water is 4:1.5:1.5:2:6:15:0.8:0.8:0.3:100.
[0075] Comparative Example 3:
[0076] The method for biodegrading antibiotics from livestock and poultry waste described in this comparative example differs from the method described in Example 1 only in that, in step (1), a mixture of round-brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing bacteria, and lactic acid bacteria is dissolved in water, and then glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate are added and stirred evenly to obtain bacterial agent A; the mass ratio of the round-brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing bacteria, lactic acid bacteria, glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, magnesium sulfate, and water is 4:1.5:6:15:0.8:0.8:0.3:100.
[0077] Comparative Example 4:
[0078] The method for biodegrading antibiotics from livestock and poultry waste described in this comparative example differs from the method described in Example 1 only in that step (4) is omitted. In step (5), bacterial agent C is added to the mixture A obtained in step (3) and reacted for 3 hours under stirring conditions at a speed of 80 r / min, and then allowed to stand for 45 minutes and filtered to obtain a mixture B free of antibiotics and heavy metals; the mass ratio of the mixture A to the bacterial agent C is 10:0.5; and the bacterial agent C is obtained by mixing yeast and rhodococcus in a mass ratio of 10:1.5.
[0079] Comparative Example 5:
[0080] The method for biodegrading antibiotics from livestock and poultry waste described in this comparative example is different from the method described in Example 1 only in that, in step (4), the bacterial agent C is obtained by mixing yeast and Bacillus subtilis in a mass ratio of 10:1.5.
[0081] Comparative Example 6:
[0082] The method for biodegrading antibiotics from livestock and poultry waste described in this comparative example is different from the method described in Example 1 only in that, in step (4), the bacterial agent C is obtained by mixing yeast, Bacillus subtilis and Pseudomonas in a mass ratio of 10:1.5:1.5.
[0083] Experimental Example 1:
[0084] Pig manure and livestock and poultry breeding wastewater were selected for the experiment. A mixture B free of antibiotics and heavy metals was prepared according to the methods described in Examples 1 to 6 and Comparative Examples 1 to 6. The content of antibiotics and heavy metals in the mixture B was tested. At the same time, the content of antibiotics and heavy metals in the mixture obtained by mixing livestock and poultry manure and livestock and poultry breeding wastewater was tested as a control example. The specific results are shown in Table 1.
[0085] Table 1 Test results of different methods for treating the mixture of livestock and poultry manure and livestock and poultry breeding wastewater
[0086]
[0087] It can be seen from the above data that the content of antibiotics and heavy metals in the mixture B obtained according to the method described in the present application is the lowest, while the antibiotic degradation effect obtained in Comparative Examples 2 and 3 using different bacterial agent formulas and Comparative Example 1 not using sustained-release microspheres is significantly reduced, and the heavy metal removal effect obtained in Comparative Example 4 using no modified zeolite to prepare the bioflocculant and Comparative Example 5 using different bacterial agent formulas to prepare the flocculant is significantly reduced.
[0088] Experimental Example 2:
[0089] Pig manure and aquaculture wastewater from a pig farm were selected for the experiment. Liquid fertilizer was prepared according to the methods described in Examples 1 to 6 and Comparative Examples 1 to 6, and the organic matter and free amino acid contents of the obtained liquid fertilizer were detected. The specific results are shown in Table 2.
[0090] Table 2 Test results of the obtained liquid fertilizer
[0091]
[0092] It can be seen from the above data that the liquid fertilizer prepared according to the method of the present invention contains more organic matter and free amino acids, which can better provide nutrition for plants and promote plant growth.
[0093] Experimental Example 3:
[0094] A field experiment was conducted on 10 mu of farmland in the suburbs of Nanning, Guangxi. Tomatoes and sweet potatoes were planted using the liquid fertilizer prepared according to the methods described in Examples 1 to 3 and Comparative Examples 1 to 2. A commonly used compound fertilizer (15-15-15) was used as a control. The yield, yield increase rate, and yield increase of tomatoes and sweet potatoes were calculated at the same application rate. The specific results are shown in Table 3.
[0095] Table 3 Results of tomato and sweet potato planting experiments
[0096]
[0097] It can be seen from the above data that the liquid fertilizer prepared by the method of the present invention has good fertilizer effect and can significantly increase crop yield.
[0098] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.
Claims
1. A method for biodegrading antibiotics from livestock and poultry breeding waste, characterized by: The following steps are involved: (1) A mixture of round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus mucilaginosus, and lactic acid bacteria was dissolved in water, and then glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, and magnesium sulfate were added and stirred to obtain bacterial agent A; N-isopropylacrylamide, chitosan, and ethylene glycol dimethacrylate were mixed and dissolved in water, and then ultrasonically dispersed for 5 to 10 minutes and stirred and heated to 50 to 60°C, and then ammonium persulfate was added and reacted at a constant temperature for 3 to 5 hours, and then cooled to room temperature, and then bacterial agent A was added and mixed at a speed of 300 to 500 r / min for 1 to 2 hours, and then filtered and dried to obtain microspheres containing microorganisms; (2) Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus and Pseudomonas aeruginosa are mixed and added to an agar medium for cultivation to obtain a bacterial agent B; the mass ratio of the Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus cereus, Pseudomonas aeruginosa and the agar medium is (5-8):(3-5):(1-2):(1-2):(5-8):100; (3) Stirring and mixing livestock and poultry manure and livestock and poultry breeding wastewater, then adding the microspheres obtained in step (1) and the bacterial agent B obtained in step (2) and mixing them evenly, and then heating to 30-40° C. and treating at a constant temperature for 7-10 days to obtain a mixture A; the mass ratio of the livestock and poultry manure, livestock and poultry breeding wastewater, microspheres and bacterial agent B is 1: (3-5): (0.05-0.1): (0.05-1); (4) After mixing zeolite, vinylsilane and ethanol, the mixture is ground at 40-50°C for 5-10 minutes to obtain a modified zeolite slurry, acrylic acid is added to the modified zeolite slurry, and the mixture is stirred and reacted at 40-50°C for 1-2 hours. After cooling naturally to room temperature, the microbial agent C is added and mixed evenly to obtain a bioflocculant; the mass ratio of the zeolite, vinylsilane and ethanol is 10:(0.3-0.5):(5-10); the mass ratio of the acrylic acid, microbial agent C and modified zeolite slurry is (2-5):(1-2):10; the microbial agent C is obtained by mixing yeast and rhodococcus in a mass ratio of 10:(1-2); (5) Add the bioflocculant obtained in step (4) to the mixture A obtained in step (3), stir and react for 3 to 5 hours, then let it stand for 30 to 60 minutes and filter to obtain a mixture B free of antibiotics and heavy metals; the mass ratio of the mixture A to the bioflocculant is 10:(0.5 to 1).
2. The method for biodegrading antibiotics from livestock and poultry breeding waste according to claim 1, characterized in that: The mass ratio of the round brown nitrogen-fixing bacteria, Bacillus phosphate-solubilizing, Bacillus subtilis, Bacillus mucilaginosus, lactic acid bacteria, glucose, potassium dihydrogen phosphate, potassium monohydrogen phosphate, magnesium sulfate and water described in step (1) is (3-5): (1-2): (1-2): (1-3): (5-8): (10-20): (0.5-1): (0.5-1): (0.2-0.5):
100.
3. The method for biodegrading antibiotics from livestock and poultry breeding waste according to claim 1, characterized in that: The drying in step (1) is carried out at 40-50° C. for 3-5 hours.
4. The method for biodegrading antibiotics from livestock and poultry breeding waste according to claim 1, characterized in that: The livestock and poultry manure and livestock and poultry breeding wastewater described in step (3) are stirred and mixed, and then sterilized at 120-150° C., and then the microspheres and bacterial agent B are added for treatment.
5. The method for biodegrading antibiotics from livestock and poultry breeding waste according to claim 1, characterized in that: In step (5), the bioflocculant obtained in step (4) is added to the mixed material A obtained in step (3) and the mixture is stirred at a speed of 50 to 100 r / min for 3 to 5 hours.
6. Use of the method for biodegrading antibiotics from livestock and poultry breeding waste according to any one of claims 1 to 5, characterized in that: The obtained mixture B is used to prepare liquid fertilizer. Specifically, the mixture B, bagasse, water and fermentation agent are mixed and fermented for 20 to 30 days to obtain liquid fertilizer; the mass ratio of the mixture B, bagasse, water and composite fermentation agent is (10 to 15): (20 to 30): 50: (1 to 5).
7. The use according to claim 6, characterized in that: The leavening agent comprises the following components in parts by weight: 10-15 parts of yeast, 10-15 parts of lactic acid bacteria, 3-5 parts of cellulase and 3-5 parts of hemicellulase.
Citation Information
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