A method for preventing and controlling cucumber mosaic virus-induced disease in cut lily leaves

Abstract

The present invention provides a method for preventing and controlling cucumber mosaic virus-induced leaf disease in cut lilies, characterized by comprising the following steps: 1) germinating lily bulbs, 2) planting the lily bulbs, 3) watering the lily bulbs, 4) watering the lily bulbs, 5) irrigating the lily bulbs with a microbial fertilizer solution, and 6) irrigating the lily bulbs with the microbial fertilizer solution, thereby obtaining cut lily plants. This method maximizes the lily plants' resistance and immunity to cucumber mosaic virus, reducing the incidence of cucumber mosaic virus to below 5%. This method maximizes the commercial value of lily bulbs carrying cucumber mosaic virus, avoids the significant economic losses to producers caused by the destruction of such bulbs or plants, and reduces fertilizer usage by over 30%. The microbial fertilizer used is environmentally friendly, ensuring a green and environmentally friendly cultivation process and overall quality assurance for cut lilies.

Description

Technical Field

[0001] The invention relates to a flower virus prevention and control method, in particular to a method for preventing and controlling cucumber mosaic virus-induced cut lily leaf disease, and belongs to the technical field of plant virus prevention and control. Technical Background

[0002] Lily is one of my country's five major fresh cut flower varieties. Lily bulbs are produced through asexual propagation, a process that takes a long time to cultivate, typically three years for a seed bulb to grow into a flowering bulb with a circumference of 14 cm or larger. Because lily bulbs spend their final two years growing in open fields, they are not only susceptible to insects and pathogens in open-air environments, which can cause the bulbs to carry viruses. Furthermore, the large amount of viruses accumulated by the mother bulbs during long periods of asexual propagation can also be transferred to the seed bulbs, further contaminating the bulbs with viruses. Infected lily plants exhibit deformities such as dwarfing, twisted or flattened stems, and clumps, curling, yellowing, or mottled leaves. These conditions affect the quality of cut lilies, reducing their economic value and severely impacting the income of flower growers and growers. Currently, over ten lily viruses have been reported both domestically and internationally. As lily bulb cultivation expands worldwide, the number of lily viruses is increasing, and they are rapidly spreading across lily-growing regions. The cut lily 'Shine on', also known as 'Shine on' in English, is a cut flower variety of the OT lily hybrid line (a cross between Oriental lily (O) and Trumpet lily (T)). 'Shine on' is both cold-resistant and heat-resistant, but it has poor virus resistance and is susceptible to cucumber mosaic virus (CMV), which causes lesions on its leaves, affecting the ornamental value of its branches and preventing it from being sold as a commercial product. Furthermore, there is currently no effective pesticide to cure the symptoms of CMV in lilies.

[0003] At present, there is no specific drug that can cure the lily cucumber mosaic virus. The only way to prevent and control the spread of the virus is to spray pesticides regularly before the virus is infected during cultivation management, or to remove the diseased plants in time after they show symptoms. Plants that are infected with the virus on a large scale can only be destroyed, which is a huge loss for growers. Therefore, if the symptoms of the virus can be alleviated or eliminated, in simple terms, the virus-carrying plants will not become sick and the quality of the cut flowers will not be affected, then the losses for lily cut flower producers can be minimized. Summary of the Invention

[0004] The present invention aims to provide a method for preventing and controlling cucumber mosaic virus-induced cut lily leaf disease, which is characterized by comprising the following steps:

[0005] 1) Germination of lily bulbs: lily bulbs carrying cucumber mosaic virus are subjected to accelerated germination treatment for 2 weeks in a dark environment at a temperature of 18-20°C and an air humidity of 85% to obtain germinated bulbs;

[0006] 2) Planting lily bulbs: Plant the germinated bulbs from step 1) in a peat substrate with a particle size of 20-40 mm and a pH of 6.0. After planting, water the substrate with clean water at a pH of 7.2-8.2 until it is thoroughly saturated.

[0007] 3) Water the lily bulbs: Starting from the sixth day after planting, water them with a pH of 7.2-8.2 through drip irrigation, watering every two days.

[0008] 4) Water the lily bulbs: Starting from the 14th day after planting, water them with a pH of 6.0 through drip irrigation, watering them every three days until the lily cut flowers are harvested.

[0009] 5) Water the lily bulbs with microbial fertilizer solution. From the 15th day after planting, water the substrate with Bacillus subtilis microbial fertilizer solution. Water once every 10 days. The dosage each time is: 3-6g / m 2 Substrate, and use compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 16:6:30, fertilize twice a week, each time with a fertilizer amount of 1.71kg / mu, until the plant has flower buds with a length of more than 0.3cm;

[0010] 6) Water the lily bulbs with microbial fertilizer solution. When the lily plants have flower buds, stop applying Bacillus subtilis microbial fertilizer and use Trichoderma microbial fertilizer solution to irrigate the substrate. Water once every 5 days, and the dosage each time is: 0.3-1.0g / m 2 The substrate was prepared by adding compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 15:10:30. The fertilizer was applied once a week at a rate of 3.42 kg / mu. After the flower buds showed color, the application of Trichoderma microbial fertilizer and compound fertilizer was stopped to obtain lily cut flower plants.

[0011] During the entire growth period of step 2) to step 6), the temperature of the cultivation medium is controlled at 16-25°C, the moisture content is controlled at 65%-70%, the planting environment temperature is controlled at 15-25°C, and the light intensity is controlled at 15000-25000Lx.

[0012] When planting the germinated bulbs in step 2), the thickness of the substrate below the germinated bulbs is controlled to be 15 cm, and a peat substrate with a thickness of 3 times the height of the bulbs is covered above the germinated bulbs, so that a 1 cm long lily bud is exposed on the surface of the peat substrate.

[0013] The effective bacterial content of the Bacillus subtilis microbial fertilizer in step 5) is above 1 billion / gram.

[0014] The effective bacterial content of the Trichoderma microbial fertilizer in step 6) is above 100 million per gram.

[0015] The present invention addresses the existing problem of a lack of effective drugs to cure cucumber mosaic virus-induced leaf disease in cut lilies. The invention utilizes conventional cultivation management to control lily bulbs and growing lily plants carrying cucumber mosaic virus to reduce the probability of healthy plants being infected with the virus. In particular, for plants already infected with the virus, the plant's roots are able to sense changes in the pH value of the cultivation soil or substrate, thereby awakening the plant's own immunity and preventing or minimizing the disease. This can mitigate the significant economic losses caused by the removal or large-scale destruction of plants due to the virus. This is because plants themselves have a certain degree of innate immunity to viruses. When certain factors in the environment, such as pH, change, the plant's innate immune system is activated. Specifically, when the pH changes, the cell surface peptide receptor complex of the plant's root cap tissue, acting as a sensor of extracellular pH, promotes the alkali-dependent binding of plant-stimulating peptides to their receptors, thereby increasing the plant's immunity.

[0016] Furthermore, microbial fertilizers refer to new types of fertilizers containing a large number of viable beneficial microorganisms. These fertilizers not only activate nutrients in the growing medium and promote crop nutrient absorption, but also balance the medium's pH, improve its physical and chemical properties, promote the development of plant roots, and enhance disease resistance. Bacillus subtilis and Trichoderma are two particularly suitable beneficial bacterial species for biofertilizers. Bacillus subtilis produces antibiotics that inhibit the growth of bacteria, viruses, fungi, and pathogens. Once spore-forming Bacillus subtilis enters the soil or growing medium, it quickly reactivates from its dormant state and rapidly reproduces, producing a large number of active substances such as proteases, lipases, and amylases. These substances degrade complex organic matter in the medium, thereby promoting fertilizer absorption and enhancing plant immunity. Upon contact with plant roots, Trichoderma can induce a defensive response in the host plant, directly inhibiting the growth and reproduction of harmful microorganisms and inducing the crop's own defense system to confer disease resistance. There are many types of Trichoderma, and Trichoderma harzianum is a prominent performer in microbial fertilizers. The xylanase produced by its metabolism can trigger a significant defense response in plants, leading to the production of phenolic compounds and lignin associated with disease resistance. In the cultivation medium, it improves the root microenvironment and accelerates the decomposition of organic matter, particularly toxic and harmful substances produced by continuous cropping, thus preventing the problem of repeated cropping. Microbial fertilizers are natural, non-toxic, and pollution-free, making them safe and efficient to use, without residual pesticides or side effects. This reduces the use of pesticides and is therefore effective in mitigating viral damage in cut lily cultivation.

[0017] The advantages and technical effects of the present invention are:

[0018] 1) Effectively control the symptoms of lily bulbs carrying cucumber mosaic virus, greatly reducing the number of lily plants showing symptoms of cucumber mosaic virus, ensuring that marketable lily cut flowers have excellent quality, maximizing the commercial value of lily bulbs carrying cucumber mosaic virus, and avoiding the huge economic losses to producers caused by the destruction of these bulbs or plants.

[0019] 2) Through the technical solution of the present invention, the resistance and immunity of lily plants to cucumber mosaic virus are stimulated to the greatest extent, lily cut flowers that do not show symptoms of cucumber mosaic virus are obtained, and the incidence rate of cucumber mosaic virus is reduced to below 5%.

[0020] 3) The application of microbial fertilizer can greatly reduce the amount of fertilizer used in formula fertilization, and the amount of fertilizer used can be saved by more than 30%.

[0021] 4) During the entire cultivation process, only clean water is used to regulate the pH value of the substrate. The microbial fertilizer used is harmless to the environment, and the cultivation process is green and environmentally friendly.

[0022] The present invention prevents lily cut flower plants carrying cucumber mosaic virus from showing symptoms of the disease by regulating the pH value of the substrate, applying different types of microbial fertilizers in steps, controlling the temperature and moisture content of the cultivation substrate during the growth period, and controlling the temperature and light intensity of the planting environment, thereby greatly reducing the number of lily plants showing symptoms of cucumber mosaic virus, ensuring the overall quality of the lily cut flowers, and retaining the commercial value of the lily cut flowers. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 These are leaves from lily plants showing symptoms of the disease, obtained by conventionally planting 'Shining' lily bulbs carrying cucumber mosaic virus.

[0024] Figure 2 is with Figure 1 The invention relates to leaves of lily plants without disease obtained by planting 'Shining' lily bulbs carrying cucumber mosaic virus from the same source using the method of the present invention. Implementation Method

[0025] The present invention will be further described below with reference to the embodiments. Example

[0026] This embodiment 1 is applied to the prevention and control of symptoms of cucumber mosaic virus in cut lily 'Shining', and includes the following steps:

[0027] 1) Lilium 'Shining' bulbs infected with cucumber mosaic virus were germinated for 2 weeks in a dark environment at 18°C and 85% humidity to obtain buds with a length of 5 cm.

[0028] 2) Plant the germinated bulbs from step 1) in a peat substrate with a particle size of 20-40 mm and a pH of 6.0, with the substrate below the bulbs 15 cm thick and covered with a peat substrate three times the height of the bulbs, leaving 1 cm of lily buds exposed on the surface of the peat substrate. After planting, water the substrate with clean water at a pH of 7.2 until it is thoroughly watered.

[0029] 3) Starting from the 6th day after planting, water the plants with a pH of 7.2 through drip irrigation, watering every 2 days;

[0030] 4) Starting from the 14th day after planting, water the lily plants with a pH of 6.0 by drip irrigation, watering them once every three days until the lily cut flowers are harvested;

[0031] 5) From the 15th day after planting, water the substrate with Bacillus subtilis microbial fertilizer solution, once every 10 days, and the amount of fertilizer used each time is: 3g / m 2 The substrate was prepared by applying compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 16:6:30 twice a week at a rate of 1.71 kg / mu until flower buds with a length of more than 0.3 cm appeared on the plants. The effective bacterial content of the Bacillus subtilis microbial fertilizer was 1 billion / gram.

[0032] 6) When the lily plants begin to flower bud, stop applying Bacillus subtilis microbial fertilizer and use Trichoderma microbial fertilizer solution to irrigate the substrate. Irrigate once every 5 days, and the amount of fertilizer used each time is: 0.3g / m 2 The substrate was prepared by adding compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 15:10:30. Fertilization was carried out once a week at a rate of 3.42 kg / mu. After the flower buds showed color, the application of Trichoderma microbial fertilizer and compound fertilizer was stopped. The 'Shining' lily cut flower plants with normal appearance were obtained. The effective bacterial content of the Trichoderma microbial fertilizer was 100 million / gram.

[0033] 7) During the entire growing period from step 2) to step 6), the temperature of the cultivation medium was controlled at 16°C, the moisture content was controlled at 65%, and the temperature of the planting environment was controlled at 15°C, and the light intensity was controlled at 25,000 Lx. Example

[0034] This Example 2 is applied to the prevention and control of symptoms of cucumber mosaic virus in cut lily 'Shining', and includes the following steps:

[0035] 1) Lilium 'Shining' bulbs infected with cucumber mosaic virus were germinated for 2 weeks in a dark environment at 20°C and 85% humidity to obtain buds with a length of 5 cm.

[0036] 2) Plant the germinated bulbs from step 1) in a peat moss substrate with a particle size of 20-40 mm and a pH of 6.0, with the substrate below the bulbs 15 cm thick and covered with a peat substrate three times the height of the bulbs, leaving 1 cm of lily buds exposed on the surface of the peat substrate. After planting, water the substrate with clean water at a pH of 8.2 until it is thoroughly watered.

[0037] 3) Starting from the 6th day after planting, water the plants with a pH of 8.2 through drip irrigation, watering every 2 days;

[0038] 4) Starting from the 14th day after planting, water the lily plants with a pH of 6.0 by drip irrigation, watering them once every three days until the lily cut flowers are harvested;

[0039] 5) From the 15th day after planting, water the substrate with Bacillus subtilis microbial fertilizer solution, once every 10 days, with a dosage of 6g / m 2 The substrate was prepared by applying compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 16:6:30 twice a week at a rate of 1.71 kg / mu until flower buds with a length of more than 0.3 cm appeared on the plants. The effective bacterial content of the Bacillus subtilis microbial fertilizer was 1 billion / gram.

[0040] 6) When the lily plants begin to flower bud, stop applying Bacillus subtilis microbial fertilizer and use Trichoderma microbial fertilizer solution to irrigate the substrate. Irrigate once every 5 days, with a dosage of 1.0 g / m 2 The substrate was prepared by adding compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 15:10:30. Fertilization was carried out once a week at a rate of 3.42 kg / mu. After the flower buds showed color, the application of Trichoderma microbial fertilizer and compound fertilizer was stopped. The 'Shining' lily cut flower plants with normal appearance were obtained. The effective bacterial content of the Trichoderma microbial fertilizer was 100 million / gram.

[0041] 7) During the entire growing period from step 2) to step 6), the temperature of the cultivation medium was controlled at 25°C, the moisture content was controlled at 70%, and the temperature of the planting environment was controlled at 25°C, and the light intensity was controlled at 15,000 Lx. Example

[0042] This Example 3 is applied to the prevention and control of symptoms of cucumber mosaic virus in cut lily 'Shining', and includes the following steps:

[0043] 1) Lilium 'Shining' bulbs infected with cucumber mosaic virus were germinated for 2 weeks in a dark environment at 19°C and 85% humidity to obtain germinated bulbs with buds 5 cm long.

[0044] 2) Plant the germinated bulbs from step 1) in a peat substrate with a particle size of 20-40 mm and a pH of 6.0, with the substrate below the bulbs 15 cm thick and covered with a peat substrate three times the height of the bulbs, leaving 1 cm of lily buds exposed on the surface of the peat substrate. After planting, water the substrate with clean water at a pH of 7.8 until it is thoroughly watered.

[0045] 3) Starting from the 6th day after planting, water the plants with a pH of 7.8 through drip irrigation, watering every 2 days;

[0046] 4) Starting from the 14th day after planting, water the lily plants with a pH of 7 by drip irrigation, watering them once every three days until the lily cut flowers are harvested;

[0047] 5) From the 15th day after planting, water the substrate with Bacillus subtilis microbial fertilizer solution, once every 10 days, with a dosage of 4g / m 2 The substrate was prepared by applying compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 16:6:30 twice a week at a rate of 1.71 kg / mu until flower buds with a length of more than 0.3 cm appeared on the plants. The effective bacterial content of the Bacillus subtilis microbial fertilizer was 1 billion / gram.

[0048] 6) When the lily plants begin to flower bud, stop applying Bacillus subtilis microbial fertilizer and use Trichoderma microbial fertilizer solution to irrigate the substrate. Irrigate once every 5 days, and the dosage each time is: 0.5g / m 2 The substrate was prepared by adding compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 15:10:30. Fertilization was carried out once a week at a rate of 3.42 kg / mu. After the flower buds showed color, the application of Trichoderma microbial fertilizer and compound fertilizer was stopped. The 'Shining' lily cut flower plants with normal appearance were obtained. The effective bacterial content of the Trichoderma microbial fertilizer was 100 million / gram.

[0049] 7) During the entire growing period from step 2) to step 6), the temperature of the cultivation medium was controlled at 20°C, the moisture content was controlled at 68%, and the temperature of the planting environment was controlled at 20°C, and the light intensity was controlled at 22,000 Lx. Example

[0050] This Example 4 is applied to the prevention and control of symptoms of cucumber mosaic virus in cut lily 'Shining', and includes the following steps:

[0051] 1) Lilium 'Shining' bulbs infected with cucumber mosaic virus were germinated for 2 weeks in a dark environment at 19°C and 85% humidity to obtain germinated bulbs with buds 5 cm long.

[0052] 2) Plant the germinated bulbs from step 1) in a peat substrate with a particle size of 20-40 mm and a pH of 6.0, with the substrate below the bulbs 15 cm thick and covered with a peat substrate three times the height of the bulbs, leaving 1 cm of lily buds exposed on the surface of the peat substrate. After planting, water the substrate with clean water at a pH of 8.0 until it is thoroughly watered.

[0053] 3) Starting from the 6th day after planting, water the plants with a pH of 8.0 by drip irrigation, once every 2 days;

[0054] 4) Starting from the 14th day after planting, water the lily plants with a pH of 6.0 by drip irrigation, watering them once every three days until the lily cut flowers are harvested;

[0055] 5) From the 15th day after planting, water the substrate with Bacillus subtilis microbial fertilizer solution, once every 10 days, with a dosage of 4g / m 2 The substrate was prepared by applying compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 16:6:30 twice a week at a rate of 1.71 kg / mu until flower buds with a length of more than 0.3 cm appeared on the plants. The effective bacterial content of the Bacillus subtilis microbial fertilizer was 1 billion / gram.

[0056] 6) When the lily plants begin to flower bud, stop applying Bacillus subtilis microbial fertilizer and use Trichoderma microbial fertilizer solution to irrigate the substrate. Irrigate once every 5 days, with a dosage of 0.8g / m 2 The substrate was prepared by adding compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 15:10:30. Fertilization was carried out once a week at a rate of 3.42 kg / mu. After the flower buds showed color, the application of Trichoderma microbial fertilizer and compound fertilizer was stopped. The 'Shining' lily cut flower plants with normal appearance were obtained. The effective bacterial content of the Trichoderma microbial fertilizer was 100 million / gram.

[0057] 7) During the entire growing period from step 2) to step 6), the temperature of the cultivation medium was controlled at 20°C, the moisture content was controlled at 66%, and the temperature of the planting environment was controlled at 20°C, and the light intensity was controlled at 18,000 Lx. Example

[0058] This embodiment is applied to the prevention and control of symptoms of cucumber mosaic virus in cut lily 'Shining', and includes the following steps:

[0059] 1) Lilium 'Shining' bulbs infected with cucumber mosaic virus were germinated for 2 weeks in a dark environment at 20°C and 85% humidity to obtain buds with a length of 5 cm.

[0060] 2) Plant the germinated bulbs from step 1) in a peat substrate with a particle size of 20-40 mm and a pH of 6.0, with the substrate below the bulbs 15 cm thick and covered with a peat substrate three times the height of the bulbs, leaving 1 cm of lily buds exposed on the surface of the peat substrate. After planting, water the substrate with clean water at a pH of 8.0 until it is thoroughly watered.

[0061] 3) Starting from the 6th day after planting, water the plants with a pH of 8.0 by drip irrigation, once every 2 days;

[0062] 4) Starting from the 14th day after planting, water the lily plants with a pH of 6 by drip irrigation, watering them once every three days until the lily cut flowers are harvested;

[0063] 5) From the 15th day after planting, water the substrate with Bacillus subtilis microbial fertilizer solution, once every 10 days, with a dosage of 5g / m 2 The substrate was prepared by applying compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 16:6:30 twice a week at a rate of 1.71 kg / mu until flower buds with a length of more than 0.3 cm appeared on the plants. The effective bacterial content of the Bacillus subtilis microbial fertilizer was 1 billion / gram.

[0064] 6) When the lily plants begin to flower bud, stop applying Bacillus subtilis microbial fertilizer and use Trichoderma microbial fertilizer solution to irrigate the substrate. Irrigate once every 5 days, with a dosage of 0.6g / m 2 The substrate was prepared by adding compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 15:10:30. Fertilization was carried out once a week at a rate of 3.42 kg / mu. After the flower buds showed color, the application of Trichoderma microbial fertilizer and compound fertilizer was stopped. The 'Shining' lily cut flower plants with normal appearance were obtained. The effective bacterial content of the Trichoderma microbial fertilizer was 100 million / gram.

[0065] 7) During the entire growing period from step 2) to step 6), the temperature of the cultivation medium was controlled at 18°C, the moisture content was controlled at 65%, and the temperature of the planting environment was controlled at 18°C, and the light intensity was controlled at 22,000 Lx.

[0066] To demonstrate the technical effect of the present invention, conventional methods and the present invention were used to plant 'Shining' lily bulbs infected with cucumber mosaic virus from the same source. The results are shown in the following table:

[0067] Comparison of the effects of conventional planting and the planting method of the present invention

[0068] ;

[0069] Note: The same type of fertilizer was used in the conventional method and the method of the present invention; the incidence rate was calculated as the ratio of diseased plants with lesion symptoms on leaves to the total number of infected bulbs planted.

[0070] Comparison of the effects of conventional planting and the present invention planting: Using the same source of 'Shining' lily bulbs infected with cucumber mosaic virus as materials, the conventional method and the present invention were used to plant respectively. The results showed that the incidence of cucumber mosaic virus on the leaves of the plants obtained from the infected bulbs planted by the conventional method was 100%, and the incidence of cucumber mosaic virus on the leaves of the plants obtained from the infected bulbs was 100%. Figure 1 It is obvious that the lesions on the leaves are large and very obvious; while the incidence of cucumber mosaic virus on the leaves of the plants obtained from the infected bulbs grown by the method of the present invention is only 0-4.6%, and the leaves with lesions are very few, which does not affect sales. Figure 2 .

[0071] The above shows and describes the differences between the method of the present invention and conventional methods, as well as the effects of the method of the present invention which are much superior to those of conventional methods.

[0072] Those skilled in the art will appreciate that the present invention is not limited to the foregoing embodiments. The foregoing embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preventing and controlling cucumber mosaic virus-induced disease in cut lily leaves, characterized in that The following steps are involved: 1) Germination of lily bulbs: lily bulbs carrying cucumber mosaic virus are subjected to accelerated germination treatment for 2 weeks in a dark environment at a temperature of 18-20°C and an air humidity of 85% to obtain germinated bulbs; 2) Planting lily bulbs: Plant the germinated bulbs from step 1) in a peat substrate with a particle size of 20-40 mm and a pH of 6.

0. After planting, water the substrate with clean water at a pH of 7.2-8.2 until it is thoroughly saturated. The substrate thickness below the germinating bulbs was controlled to be 15 cm, and a peat substrate with a thickness of 3 times the height of the bulbs was covered above the germinating bulbs, so that a 1 cm long lily bud was exposed on the surface of the peat substrate; 3) Water the lily bulbs: Starting from the sixth day after planting, water them with a pH of 7.2-8.2 through drip irrigation, watering every two days. 4) Water the lily bulbs: Starting from the 14th day after planting, water them with a pH of 6.0 through drip irrigation, watering every 3 days until the lily cut flowers are harvested. 5) Water the lily bulbs with microbial fertilizer solution. From the 15th day after planting, water the substrate with Bacillus subtilis microbial fertilizer solution. Water once every 10 days. The dosage each time is: 3-6g / m 2 Substrate, and use compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 16:6:30, fertilize twice a week, each time with a fertilizer amount of 1.71kg / mu, until the plant has flower buds with a length of more than 0.3cm; The effective bacterial content of Bacillus subtilis microbial fertilizer is more than 1 billion / gram; 6) Water the lily bulbs with microbial fertilizer solution. When the lily plants have flower buds, stop applying Bacillus subtilis microbial fertilizer and use Trichoderma microbial fertilizer solution to irrigate the substrate. Water once every 5 days, and the dosage each time is: 0.3-1.0g / m 2 substrate, and compound fertilizer with a nitrogen, phosphorus and potassium mass ratio of 15:10:30, fertilizing once a week, each fertilizer amount is: 3.42kg / mu, until the flower buds show color, stop applying Trichoderma microbial fertilizer and compound fertilizer, and obtain lily cut flower plants; The effective bacterial content of Trichoderma microbial fertilizer is more than 100 million per gram.

2. The method for preventing and controlling cucumber mosaic virus-induced cut lily leaf disease according to claim 1, wherein: During the entire growth period of step 2) to step 6), the temperature of the cultivation medium is controlled at 16-25°C, the moisture content is controlled at 65%-70%, the planting environment temperature is controlled at 15-25°C, and the light intensity is controlled at 15000-25000Lx.

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