A seedling medium

Abstract

The present invention belongs to the technical field of seedling substrates, and specifically belongs to a seedling substrate. A seedling substrate, in terms of mass percentage, the seedling substrate is mixed with the following components: 90-95% of a substrate component, 0.5-0.75% of a biological agent component, and the remainder of a fertilizer component; wherein the substrate component is mixed with vermiculite, perlite, peat soil, and coconut bran in a mass ratio of 4:1:1:2; the biological agent is mixed with Bacillus brevis and fluopyram; the fertilizer component is mixed with potassium dihydrogen phosphate, potassium sulfate, and magnesium sulfate in a mass ratio of 8:4:3. The seedling substrate of the present invention consists of three parts: a substrate component, a biological agent, and a fertilizer component. The substrate component can provide an excellent growth environment for crop seedlings, the fertilizer component can provide nutrients for crop seedlings, and the biological agent can effectively prevent the influence of southern root-knot nematodes on crop seedlings, thereby ensuring the survival and healthy growth of crop seedlings.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and in particular relates to a seedling culture medium. Background Art

[0002] Seedling substrates can be used to grow seedlings of crops such as cucumbers, but conventional seedlings of crops such as cucumbers are susceptible to damage by southern root-knot nematodes. Southern root-knot nematodes mainly destroy the root structure of plants by invading wounds and forming nodular root knots, affecting the normal metabolic activities of plants, and causing other pathogens to infect, causing the growth of plants to weaken or even die, thereby reducing crop yields or even causing crop failure.

[0003] However, many seedling substrates only provide nutrients and a normal environment for crop seedlings, and do not prevent the harm of southern root-knot nematodes. If an appropriate amount of pesticide components that kill southern root-knot nematodes are added to the seedling substrate, this will undoubtedly prevent and control southern root-knot nematodes to a certain extent, ensure the survival and normal growth of crop seedlings such as cucumbers, and then be conducive to the smooth development of seedling work. In the prior art, the medicaments for preventing and controlling southern root-knot nematodes include abamectin, emamectin benzoate, thiathiazolyl and carbosulfan, etc., but due to the accumulation of southern root-knot nematode resistance, the efficacy of a single medicament is limited under conventional dosage, therefore, it is necessary to use a compound or mixed agent for preventing and controlling root-knot nematodes instead. There is no related report on adding short stable bacillus and fluopyram mixed in the seedling substrate.

[0004] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0005] The purpose of the present invention is to provide a seedling raising matrix, which can effectively prevent the influence of southern root-knot nematodes on crop seedlings, and further can ensure the normal growth of crop seedlings.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A seedling raising matrix, wherein the seedling raising matrix is ​​mixed with the following components in percentage by mass: 90-95% of a matrix component, 0.5-0.75% of a biological agent component, and the remainder of a fertilizer component; wherein the matrix component is mixed with vermiculite, perlite, peat soil, and coconut bran in a mass ratio of 4:1:1:2;

[0008] The biological bacterial agent is prepared by mixing Bacillus brevis and fluopyram;

[0009] The fertilizer component is prepared by mixing potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate in a mass ratio of 8:4:3.

[0010] Preferably, the Bacillus brevis is a Bacillus brevis stock drug with 30 billion spores / g; and the fluopyram is a fluopyram suspension concentrate with a mass fraction of 41.7%.

[0011] Preferably, the mass ratio of Bacillus brevis to fluopyram is 1-7:19-1.

[0012] Preferably, the mass ratio of Bacillus brevis to fluopyram is 1:7.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] (1) The seedling culture medium of the present invention is composed of three parts: a matrix component, a biological agent and a fertilizer component. The matrix component can provide an excellent growth environment for crop seedlings, the fertilizer component can provide nutrients for crop seedlings, and the biological agent can effectively prevent the influence of southern root-knot nematodes on crop seedlings, thereby ensuring the survival and healthy growth of crop seedlings.

[0015] (2) The biological bacterial agent of the present invention is a mixture of Bacillus brevis and fluopyram. When the two are mixed within a certain mass ratio range, they have a synergistic effect on southern root-knot nematodes, thereby improving the control effect on southern root-knot nematodes. DETAILED DESCRIPTION

[0016] The following is a clear and complete description of the technical solution of the patent of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0017] In the following examples, vermiculite, perlite, peat soil and coconut husk are commercially available common seedling substrate raw materials, and there are no special requirements.

[0018] Example 1 :Indoor biological activity of a mixture of Bacillus brevis and fluopyram

[0019] 1. Test object: Southern root-knot nematode (Meloidogyne incognita)

[0020] 2. Test reagents

[0021] 30 billion spores / g of Bacillus brevis parent drug (Zhenjiang Runyu Biotechnology Development Co., Ltd., registration number: PD20130367)

[0022] 41.7% Fluopyram SC (Bayer AG, registration number: PD20121664)

[0023] Brevibacillus and fluopyram are prepared into 1000 mg / L (mass fraction of Brevibacillus stock drug is 100%) according to the effective ingredients, and then multiple groups of proportions are set, and each single agent and proportioned mixture is diluted with a 0.1% mass fraction Tween-80 aqueous solution into 5 mass concentration gradients for standby use.

[0024] 3. Test method: (Refer to "NT / Y 1833.1-2009 Guidelines for Indoor Bioassay Tests of Pesticides - Nematicides Part 1 - Tests for Inhibition of Plant Pathogenic Nematodes - Insect Immersion Method")

[0025] 3.1 Pick the root-knot nematode eggs from the cucumber roots, wash them with clean water, and place them on wet filter paper in a culture dish. Incubate them at 25°C to obtain second-instar larvae of the same age. Elute the cultured root-knot nematodes with water, filter, centrifuge at 1000r / min for 2min, discard the supernatant, add water, centrifuge again, and finally resuspend the root-knot nematodes with water to 200 heads / mL for later use.

[0026] 3.2 Use a pipette to pipette 3 mL of the drug solution into the test tube from low concentration to high concentration, and then pipette 3 mL of the prepared nematode suspension into the test tube to mix the drug solution and the nematode suspension in equal amounts. Use a pipette to transfer a certain volume of the mixed solution into the small holes of a 24-well biochemical test plate, cover it, and culture it at a constant temperature of 25°C for 24 hours. At the same time, set the treatment without drug as the blank control.

[0027] 3.3 Take 1 mL of the mixed solution from each treatment and observe the death of nematodes under a dissecting microscope. The number of nematodes observed in each repetition should be no less than 100. Record the total number of nematodes investigated and the number of dead nematodes (the criterion for the death of nematodes is: the nematodes are rigid and cannot bend or move when touched with a hairpin or bamboo needle). Calculate the mortality rate and adjusted mortality rate of each treatment based on the survey data.

[0028]

[0029] In the above formula: P--mortality rate, unit is %; K--number of dead insects; N--total number of insects treated.

[0030]

[0031] In the above formula: P 1 --Adjusted mortality rate, in %; P t --Treatment mortality rate, in %; P 0 --Blank control mortality rate, in %.

[0032] 4. Data analysis: DPS software was used to perform regression analysis on the logarithmic values ​​of the concentration of each treatment agent and the corrected mortality probability value of each treatment, and the LC of each treatment agent was calculated. 50 , and the co-toxicity coefficient (CTC) was calculated according to Sun Yunpei's method.

[0033] 5. Efficacy evaluation

[0034] The synergistic effect of the drugs was evaluated based on the calculated co-toxicity coefficient (CTC), CTC ≤ 80 for antagonistic effect, 80 < CTC < 120 for additive effect, and CTC ≥ 120 for synergistic effect. The results are shown in Table 1.

[0035] Table 1 Indoor biological activity of the mixture of Bacillus brevis and fluopyram against root-knot nematodes of southern root

[0036]

[0037] As shown in Table 1, after the active ingredients of Bacillus brevis and fluopyram were mixed, the co-toxicity coefficient against southern root-knot nematodes was greater than 120 at a mass ratio of 1:19-1, showing a synergistic effect, especially when the mass ratio was 1:7. In addition, when the mass ratio was 5-7:1, the co-toxicity coefficient against southern root-knot nematodes was greater than 80 but less than 120, showing an additive effect.

[0038] Example 2

[0039] A seedling raising matrix, wherein the seedling raising matrix is ​​mixed with the following components in percentage by mass: 94% of a matrix component, 0.5% of a biological agent component, and the remainder of a fertilizer component; wherein the matrix component is mixed with vermiculite, perlite, peat soil, and coconut bran in a mass ratio of 4:1:1:2;

[0040] The biological bacterial agent is mixed with Bacillus brevis and fluopyram; the Bacillus brevis is a Bacillus brevis stock drug with 30 billion spores / g, and the fluopyram is a fluopyram suspension with a mass fraction of 41.7%; the mass ratio of the Bacillus brevis to fluopyram is 1:7;

[0041] The fertilizer component is prepared by mixing potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate in a mass ratio of 8:4:3.

[0042] Comparative Example 1

[0043] A seedling culture matrix, which is mixed by the following components in percentage by mass: 94% of a matrix component, 0.5% of Bacillus brevis and the remainder of a fertilizer component; wherein the matrix component is mixed by vermiculite, perlite, peat soil and coconut bran in a mass ratio of 4:1:1:2; the Bacillus brevis is a Bacillus brevis mother drug with 30 billion spores / gram; and the fertilizer component is mixed by potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate in a mass ratio of 8:4:3.

[0044] Comparative Example 2

[0045] A seedling raising matrix, which is mixed by the following components in percentage by mass: 94% of a matrix component, 0.5% of fluopyram and the remainder of a fertilizer component; wherein the matrix component is mixed by vermiculite, perlite, peat soil and coconut bran in a mass ratio of 4:1:1:2; the fluopyram is a fluopyram suspension concentrate with a mass fraction of 41.7%; and the fertilizer component is mixed by potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate in a mass ratio of 8:4:3.

[0046] Effects of different seedling medium on cucumber root-knot nematode disease

[0047] 1. Test seedlings

[0048] "Zhongnong No. 6" cucumber seedlings, 2-3 true leaves stage.

[0049] 2. Test seedling medium

[0050] The seedling growing substrates of Example 2, Comparative Example 1 and Comparative Example 2.

[0051] 3. Test methods

[0052] 3.1 Inoculate the southern root-knot nematodes in the test seedling medium so that the number of southern root-knot nematodes per 100g of seedling medium is 300-350

[0053] 3.2 Place the test seedling medium into plastic pots (7 cm × 7 cm × 7 cm), 200 g / pot, and then transplant the test seedlings into the plastic pots, 1 plant per pot. Set up 15 replicates for each test seedling medium.

[0054] 3.3 After 30 days of management and cultivation in the conventional way, measure the number of root nodes and calculate the number of root nodes per plant.

[0055] 4. Determination of southern root-knot nematodes in soil

[0056] Mix the soil sample thoroughly, take 100g and put it in a plastic bucket, add 1000mL of water, stir vigorously to disperse the soil lumps, let the upper liquid pass through 160-mesh to 400-mesh sieves after 1 minute, rinse with water continuously, collect the residue on the 400-mesh sieve, wash with clean water and make the volume to 50mL, draw 1mL into a petri dish and observe under a microscope. Calculate the number of nematodes per 100 soil.

[0057] 5. Test results

[0058] The results are shown in Table 2.

[0059] Table 2 Effects of different seedling medium on cucumber root knot nematode disease

[0060] Test seedling medium Number of nematodes in the seedling substrate before transplanting (nematodes / 100g soil) Number of root knots per plant after 30 days Example 2 341 37.67 Comparative Example 1 329 91.00 Comparative Example 2 334 72.40

[0061] As shown in Table 2, compared with the use of fluopyram or Bacillus brevis alone, the seedling culture medium of the present invention can effectively prevent the influence of southern root-knot nematodes on crop seedlings.

[0062] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A seedling culture medium, characterized in that: The seedling culture medium is mixed with the following components in percentage by mass: 90-95% of a matrix component, 0.5-0.75% of a biological agent component, and the remainder of a fertilizer component; wherein the matrix component is mixed with vermiculite, perlite, peat soil, and coconut bran in a mass ratio of 4:1:1:2; The biological bacterial agent is prepared by mixing Bacillus brevis and fluopyram; The fertilizer component is composed of potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate mixed in a mass ratio of 8:4:3; wherein the short-stable Bacillus is a short-stable Bacillus mother drug with 30 billion spores / gram; the fluopyram is a fluopyram suspension with a mass fraction of 41.7%; the mass ratio of the short-stable Bacillus to fluopyram is 1-7:19-1.

2. The seedling culture medium according to claim 1, characterized in that The mass ratio of Bacillus brevis to fluopyram is 1:7.

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