Mouthwash and preparation method thereof

Abstract

The present invention relates to a mouthwash with antiviral effect and a preparation method thereof, and belongs to the technical field of daily chemical products. The mouthwash components of the present invention include a compound Chinese medicine composition, ethanol, glycerol, salt, peppermint oil, and purified water, and the raw materials for preparing the compound Chinese medicine composition include honeysuckle, forsythia and scutellaria. The mouthwash with antiviral effect provided by the present invention is all-natural, safe and healthy through the combination of the above components, can maximize the antiviral function, and also has anti-inflammatory and antibacterial effects. The mouthwash preparation process of the present invention is simple, low in cost, safe and stable in effect, and the prepared product has no toxic side effects.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical products, and particularly relates to a mouthwash with antiviral effect and a preparation method thereof. Background Art

[0002] Due to the continuous spread of influenza virus and its unpredictability and resistance, many people have been infected with influenza virus. Influenza is highly contagious and difficult to cure, and it is difficult to prevent in daily life. Once infected with influenza virus, it will cause certain harm to the human body. Most influenza viruses enter the human body through the oropharyngeal cavity. Mouthwash is a new product of the technological era. Its main function is oral health care and prevention. It is easy to use, clean and hygienic, and is favored by people.

[0003] CN108186491A provides a mouthwash containing bamboo vinegar and a preparation method thereof. The mouthwash of the present invention comprises 8-12 parts of bamboo vinegar, 0.01-0.03 parts of disodium ethylenediaminetetraacetic acid, 0.01-0.03 parts of sodium pyrophosphate, 0.005-0.02 parts of sweetener, 8-12 parts of sorbitol, 68-72 parts of water, 0.6-1 parts of RH40 solubilizer, 0.01-0.03 parts of menthyl amide, 0.05-0.1 parts of preservative, 0.1-0.4 parts of flavor, 7-12 parts of edible alcohol and 1-3 parts of edible pigment. The mouthwash of the present invention can effectively resist bacteria, inhibit dental plaque, prevent and alleviate oral problems such as periodontal disease, has good cleaning and care of the oral cavity, makes the breath fresh, and has bright appearance and color, and a stable formula system.

[0004] CN109303707A provides a mouthwash for effectively inhibiting bad breath in the mouth and a preparation method thereof. The main raw materials are chlorine dioxide, cetylpyridinium chloride and zinc citrate, and the solvent is distilled water. The mouthwash provided by the invention has the effect of effectively inhibiting bad breath in the mouth for a long time, has less side effects, and has no obvious irritation to the oral mucosa.

[0005] However, the above products are only used for oral cleaning or antibacterial and anti-inflammatory problems, and their antiviral effects need to be improved. Therefore, it is necessary to develop a mouthwash that is safe, healthy, non-toxic and has no side effects and can maximize the role of preventing the spread of influenza viruses. Summary of the invention

[0006] In view of the deficiencies in the prior art, the object of the present invention is to provide a mouthwash with antiviral effect and a preparation method thereof.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a mouthwash with antiviral effect, wherein the components of the functional mouthwash include a compound Chinese medicine composition, ethanol, glycerin, salt, peppermint oil, and purified water;

[0009] The raw materials for preparing the compound Chinese medicine composition include honeysuckle, forsythia and scutellaria.

[0010] The compound Chinese medicine composition in the mouthwash provided by the invention has the effects of anti-virus and anti-inflammatory, and plays a key role in blocking the spread of influenza viruses entering the oral cavity; the ethanol aqueous solution can assist the compound honeysuckle to enhance the anti-virus, bactericidal and anti-inflammatory effects; glycerol can form a film in the oral cavity after people use the mouthwash, and enhance its effect of preventing spread. The mouthwash can not only protect the cleanliness of the oral cavity through the combination of the above components, but also has the effects of anti-bacterial, anti-inflammatory and anti-viral, and especially has a good inhibitory effect on influenza viruses entering the oral cavity.

[0011] Preferably, the components of the functional mouthwash include, by weight, 5-15 parts of the compound Chinese medicine composition, 1-5 parts of ethanol, 5-10 parts of glycerol, 0.1-1 parts of salt, 0.5-2 parts of peppermint oil, 0.01-1 parts of sodium benzoate, and purified water.

[0012] The amount of the compound Chinese medicine composition added to the components of the mouthwash with antiviral effect of the present invention can be 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts or 14 parts, etc.;

[0013] The amount of ethanol added can be 1 part, 2 parts, 3 parts, 4 parts or 5 parts, etc.;

[0014] The amount of glycerol added can be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts, etc.;

[0015] The amount of salt added can be 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts or 0.9 parts, etc.;

[0016] The added amount of the peppermint oil can be 0.6 parts, 0.8 parts, 1 parts, 1.2 parts, 1.5 parts, 1.7 parts or 1.9 parts, etc.

[0017] Preferably, the components of the mouthwash with antiviral effect include 8-12 parts of the compound Chinese medicine composition, 1-3 parts of ethanol, 7-9 parts of glycerol, 0.1-0.4 parts of salt, 0.8-1.2 parts of peppermint oil, and purified water by weight.

[0018] Preferably, the mass ratio of honeysuckle, forsythia and scutellaria is (4-5):(1-2):(1-2).

[0019] Wherein "4-5" can be 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8 or 4.9, etc.;

[0020] The first "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0021] The first "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.

[0022] In the compound Chinese medicine composition of the present invention, honeysuckle is praised as a good medicine for clearing away heat and detoxifying. It is sweet, cold and aromatic, and can clear away heat without hurting the stomach. The aroma is penetrating and can dispel evil. Honeysuckle can not only dissipate wind-heat, but also clear away blood poison. It is used for various febrile diseases, such as fever, rash, spots, heat-toxic sores, sore throat and other symptoms, and all have significant effects; Scutellaria baicalensis has the effect of clearing away heat and drying dampness. It cools blood and calms the fetus, treats fetal movement disorder in pregnant women, and cools blood and calms the fetus. It is mainly used to treat febrile diseases. The special characteristics of febrile diseases are rapid onset, rapid changes, and multiple changes. In addition to fever, most of them have high heat potential. Taking Scutellaria baicalensis can relieve the condition; Forsythia suspensa can inhibit Salmonella typhi, Salmonella paratyphi, Escherichia coli, Shigella dysenteriae, Corynebacterium diphtheriae, Vibrio cholerae, Staphylococcus aureus, Streptococcus, etc. The combination of the three in a specific mass ratio can have a good effect on eliminating the bacteria in the oral cavity, and has a good blocking effect on viruses entering the mouth, thereby making the compound Chinese medicine composition have more excellent antiviral and anti-inflammatory effects.

[0023] Preferably, the raw materials for preparing the compound Chinese medicine composition further include any one of Pueraria lobata, Ligusticum chuanxiong or Gastrodia elata, or a combination of at least two of them.

[0024] Preferably, the raw materials for preparing the compound Chinese medicine composition are a combination of honeysuckle, forsythia, scutellaria, gastrodia and ligusticum.

[0025] Preferably, the mass ratio of honeysuckle, forsythia, scutellaria, gastrodia and ligusticum chuanxiong is (4-5):(1-2):(1-2):(0.1-1):(0.1-1).

[0026] The two "0.1-1" can independently be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.

[0027] As a preferred technical solution of the present invention, when the compound Chinese medicine composition is a combination of honeysuckle, forsythia, scutellaria, gastrodia and chuanxiong, gastrodia and chuanxiong can better enhance the antibacterial and anti-influenza virus capabilities of the compound Chinese medicine composition.

[0028] Specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0029] Preferably, the compound Chinese medicine composition is prepared by a preparation method comprising the following steps:

[0030] (1) soaking, boiling, filtering and concentrating each Chinese medicine raw material in purified water to obtain each Chinese medicine concentrate;

[0031] (2) Mixing and stirring the concentrated Chinese medicine solutions to obtain the compound Chinese medicine composition.

[0032] Preferably, the soaking time in step (1) is independently 20-40 min, for example, 22 min, 24 min, 26 min, 28 min, 30 min, 32 min, 34 min, 36 min or 38 min, etc.

[0033] Preferably, the number of decoctions in step (1) is independently 1-3 times, for example, 1 time, 2 times or 3 times.

[0034] Preferably, the decoction time is independently 1.5-3 h, for example, 1.8 h, 2 h, 2.2 h, 2.4 h, 2.6 h or 2.8 h.

[0035] Preferably, the density of each Chinese medicine concentrate concentrated in step (2') is independently 1.05-1.2 g / mL, for example, 1.07 g / mL, 1.08 g / mL, 1.1 g / mL, 1.12 g / mL, 1.14 g / mL, 1.16 g / mL or 1.18 g / mL.

[0036] The preparation method of the compound Chinese medicine composition of the present invention is simple and easy to operate, easy to industrialize and mass produce, and has excellent practicality. Through multiple decoctions, water can better enter the Chinese medicine components, so that the medicinal ingredients are better dissolved in the water, thereby improving the utilization rate of the Chinese medicine components.

[0037] The mixing and stirring temperature in step (2) is 80-90°C, for example, it can be 81°C, 82°C, 83°C, 84°C, 85°C, 86°C, 87°C, 88°C or 89°C.

[0038] Preferably, the mixing and stirring time in step (2) is 10-30 min, for example, it can be 12 min, 15 min, 18 min, 20 min, 22 min, 25 min or 28 min.

[0039] Preferably, the components of the mouthwash with antiviral effect also include a pH regulator and / or a preservative.

[0040] Preferably, the pH adjuster comprises citric acid.

[0041] Preferably, the preservative comprises sodium benzoate.

[0042] Specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0043] In a second aspect, the present invention provides a method for preparing a mouthwash with antiviral effect as described in the first aspect, the preparation method comprising: mixing and stirring the compound Chinese medicine composition, ethanol, glycerol, salt, and peppermint oil in purified water, and allowing to stand to obtain the mouthwash with antiviral effect.

[0044] Preferably, the mixing further comprises adding a pH regulator and / or a preservative.

[0045] Preferably, the mixing is carried out at a temperature of 22-25°C, for example, the temperature may be 22°C, 23°C, 24°C or 25°C.

[0046] Preferably, the stirring time is 0.5-1.5 h, for example, 0.6 h, 0.8 h, 1 h, 1.2 h or 1.4 h.

[0047] Preferably, the standing time is 8-12 h, for example, it can be 8.5 h, 9 h, 9.5 h, 10 h, 10.5 h, 11 h or 11.5 h.

[0048] Specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0049] Compared with the prior art, the present invention has the following beneficial effects:

[0050] 1. The compound honeysuckle in the mouthwash provided by the present invention has the effects of antiviral and anti-inflammatory, has a good effect of eliminating the bacteria in the oral cavity, protects the cleanliness of the oral cavity, and has a certain blocking effect on the bacteria entering the oral cavity, and plays a key role in the spread of influenza virus; the ethanol aqueous solution can assist the compound honeysuckle to enhance the antiviral, bactericidal and anti-inflammatory effects; glycerol can form a film in the oral cavity after people use the mouthwash, and enhance its role in preventing the spread. The mouthwash can not only protect the cleanliness of the oral cavity through the combination of the above components, but also has the effects of antibacterial, anti-inflammatory and antiviral, especially has a good inhibitory effect on influenza virus entering the oral cavity.

[0051] 2. The compound Chinese medicine composition of the present invention can effectively eliminate the bacteria in the oral cavity by using honeysuckle, forsythia and scutellaria, and has a good blocking effect on viruses entering the oral cavity, thereby making the compound Chinese medicine composition have better antiviral and anti-inflammatory effects.

[0052] 3. The preparation method of the mouthwash with antiviral effect provided by the present invention is simple and easy to operate, easy to industrialize and mass produce, and has excellent practicality. DETAILED DESCRIPTION

[0053] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0054] The peppermint oil described in the present invention was purchased from Jilin Huanglong Food Co., Ltd.

[0055] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0056] Example 1

[0057] The present embodiment provides a mouthwash with antiviral effect, wherein the components of the functional mouthwash include, by weight, 10 parts of a compound Chinese medicine composition, 2 parts of 75% ethanol, 8 parts of glycerol, 0.05 parts of citric acid, 0.2 parts of salt, 1 part of peppermint oil, 0.04 parts of sodium benzoate, and purified water supplemented to 100 parts;

[0058] The compound Chinese medicine composition is a product prepared from honeysuckle, scutellaria baicalensis, forsythia suspensa, gastrodia elata and ligusticum chuanxiong in a mass ratio of 3:1:1:0.5:0.3.

[0059] The preparation method of the mouthwash with antiviral effect is:

[0060] (1) adding 9 times the amount of purified water to soak for 30 minutes, heating and then boiling for the first time for 2 hours, filtering and collecting the first filtrate and the filter residue, adding the filter residue to 7 times the amount of purified water, heating and then boiling for the second time for 2 hours, filtering and then collecting the second filtrate, combining the first filtrate and the second filtrate, and concentrating to obtain a honeysuckle concentrate, wherein the density of the honeysuckle concentrate is 1.1 g / mL;

[0061] (2) The preparation method of the scutellaria baicalensis concentrated solution, the forsythia suspensa concentrated solution, the gastrodia elata concentrated solution or the chuanxiong concentrated solution is the same as step (1), and the density of the scutellaria baicalensis concentrated solution, the forsythia suspensa concentrated solution, the gastrodia elata concentrated solution and the chuanxiong concentrated solution is independently 1.1 g / mL;

[0062] (3) mixing and stirring the honeysuckle concentrate, scutellaria baicalensis concentrate, forsythia suspensa concentrate, gastrodia elata concentrate and ligusticum chuanxiong concentrate at 85° C. for 20 min to obtain the composite Chinese medicine composition after mixing and homogenizing;

[0063] (4) The compound Chinese medicine composition obtained in step (3) is mixed with ethanol, glycerol, citric acid, salt, peppermint oil and sodium benzoate at 23° C. according to the formula amount, and purified water is added and stirred for 1 hour. After standing for 10 hours, the mixture is filtered to obtain the mouthwash with antiviral effect.

[0064] Example 2

[0065] The present embodiment provides a mouthwash with antiviral effect, wherein the components of the functional mouthwash include, by weight, 12 parts of a compound Chinese medicine composition, 1 part of 75% ethanol, 7 parts of glycerol, 0.06 parts of citric acid, 0.4 parts of salt, 1.2 parts of peppermint oil, 0.01 parts of sodium benzoate, and purified water supplemented to 100 parts;

[0066] The compound Chinese medicine composition is a product prepared from honeysuckle, scutellaria baicalensis, forsythia suspensa, gastrodia elata and ligusticum chuanxiong in a mass ratio of 4:1:2:0.1:1.

[0067] The preparation method of the mouthwash with antiviral effect is:

[0068] (1) adding 10 times the amount of purified water to soak for 20 minutes, heating and then boiling for the first time for 3 hours, filtering and collecting the first filtrate and the filter residue, adding the filter residue to 8 times the amount of purified water, heating and then boiling for the second time for 1.5 hours, filtering and then collecting the second filtrate, combining the first filtrate and the second filtrate, and concentrating to obtain a honeysuckle concentrate, wherein the density of the honeysuckle concentrate is 1.2 g / mL;

[0069] (2) The preparation method of the scutellaria baicalensis concentrated solution, the forsythia suspensa concentrated solution, the gastrodia elata concentrated solution or the chuanxiong concentrated solution is the same as step (1), and the density of the scutellaria baicalensis concentrated solution, the forsythia suspensa concentrated solution, the gastrodia elata concentrated solution and the chuanxiong concentrated solution is independently 1.2 g / mL;

[0070] (3) mixing and stirring the honeysuckle concentrate, scutellaria baicalensis concentrate, forsythia suspensa concentrate, gastrodia elata concentrate and ligusticum chuanxiong concentrate at 90° C. for 15 minutes to obtain the composite Chinese medicine composition after mixing and homogenizing;

[0071] (4) The compound Chinese medicine composition obtained in step (3) is mixed with ethanol, glycerol, citric acid, salt, peppermint oil and sodium benzoate at 22° C. according to the formula amount, and purified water is added and stirred for 1.5 hours. After standing for 8 hours, the mouthwash with antiviral effect is filtered.

[0072] Example 3

[0073] The present embodiment provides a mouthwash with antiviral effect, wherein the components of the functional mouthwash include, by weight, 8 parts of a compound Chinese medicine composition, 3 parts of 75% ethanol, 9 parts of glycerol, 0.03 parts of citric acid, 0.1 parts of salt, 0.8 parts of peppermint oil, 0.03 parts of sodium benzoate, and purified water supplemented to 100 parts;

[0074] The compound Chinese medicine composition is a product prepared from honeysuckle, scutellaria baicalensis, forsythia suspensa, gastrodia elata and ligusticum chuanxiong in a mass ratio of 5:2:1:1:0.2.

[0075] The preparation method of the mouthwash with antiviral effect is:

[0076] (1) adding 8 times the amount of purified water to soak for 40 minutes, heating and then boiling for the first time for 1.5 hours, filtering and collecting the first filtrate and the filter residue, adding the filter residue to 6 times the amount of purified water, heating and then boiling for the second time for 2.5 hours, filtering and then collecting the second filtrate, combining the first filtrate and the second filtrate, and concentrating to obtain a honeysuckle concentrate, wherein the density of the honeysuckle concentrate is 1.05 g / mL;

[0077] (2) The preparation method of the scutellaria baicalensis concentrated solution, the forsythia suspensa concentrated solution, the gastrodia elata concentrated solution or the chuanxiong concentrated solution is the same as step (1), and the density of the scutellaria baicalensis concentrated solution, the forsythia suspensa concentrated solution, the gastrodia elata concentrated solution and the chuanxiong concentrated solution is independently 1.05 g / mL;

[0078] (3) mixing and stirring the honeysuckle concentrate, scutellaria baicalensis concentrate, forsythia suspensa concentrate, gastrodia elata concentrate and ligusticum chuanxiong concentrate at 80° C. for 25 min to obtain the composite Chinese medicine composition after mixing and homogenizing;

[0079] (4) The compound Chinese medicine composition obtained in step (3) is mixed with ethanol, glycerol, citric acid, salt, peppermint oil and sodium benzoate at 25° C. according to the formula amount, and purified water is added and stirred for 0.8 h. After standing for 12 h, the mouthwash with antiviral effect is filtered.

[0080] Example 4

[0081] This embodiment provides a mouthwash with antiviral effect. The only difference between the mouthwash and Example 1 is that the raw materials of the compound Chinese medicine composition do not include Gastrodia elata and Chuanxiong, and the reduced amounts of Gastrodia elata and Chuanxiong are added to honeysuckle, scutellaria baicalensis and forsythia suspensa in proportion. The other components are consistent with Example 1, and the preparation method refers to Example 1.

[0082] Example 5

[0083] This embodiment provides a mouthwash with antiviral effect. The only difference between the mouthwash and Example 1 is that the raw materials of the compound Chinese medicine composition do not include Gastrodia elata, and a reduced amount of Gastrodia elata is added to Chuanxiong. The other components are consistent with Example 1, and the preparation method refers to Example 1.

[0084] Example 6

[0085] This embodiment provides a mouthwash with antiviral effect. The difference between the mouthwash and Example 1 is that the raw materials of the compound Chinese medicine composition do not include Chuanxiong, and a reduced amount of Chuanxiong is added to Gastrodia elata. The other components are consistent with Example 1, and the preparation method refers to Example 1.

[0086] Example 7

[0087] The present embodiment provides a mouthwash with antiviral effect, wherein the components of the functional mouthwash include, by weight, 10 parts of a compound Chinese medicine composition, 2 parts of an ethanol aqueous solution, 8 parts of glycerol, 0.05 parts of citric acid, 0.2 parts of salt, 1 part of peppermint oil, 0.04 parts of sodium benzoate, and purified water supplemented to 100 parts;

[0088] The compound Chinese medicine composition is a product prepared from honeysuckle, scutellaria baicalensis, forsythia suspensa, gastrodia elata and ligusticum chuanxiong in a mass ratio of 3:1:1:0.5:0.3.

[0089] The preparation method of the mouthwash with antiviral effect is:

[0090] (1) adding honeysuckle, scutellaria, forsythia, gastrodia and ligusticum chuanxiong in a mass ratio of 3:1:1:0.5:0.3 to 9 times the amount of purified water and soaking for 30 minutes, heating and then boiling for the first time for 2 hours, filtering and collecting the first filtrate and the residue respectively, adding the residue to 7 times the amount of purified water, heating and then boiling for the second time for 2 hours, filtering and then collecting the second filtrate, combining the first filtrate and the second filtrate, and concentrating to obtain a compound Chinese medicine composition, wherein the density of the compound Chinese medicine composition is 1.1 g / mL;

[0091] (2) The compound Chinese medicine composition obtained in step (1) is mixed with ethanol aqueous solution, glycerol, citric acid, salt, peppermint oil and sodium benzoate at 23° C. according to the formula amount, and purified water is added and stirred for 1 hour. After standing for 10 hours, the mixture is filtered to obtain the mouthwash with antiviral effect.

[0092] Comparative Example 1

[0093] This comparative example provides a mouthwash with antiviral effect. The difference between the mouthwash and Example 1 is that the raw materials of the compound Chinese medicine composition do not include Scutellaria baicalensis, and the reduced amount of Scutellaria baicalensis is proportionally distributed to Honeysuckle and Forsythia suspensa. The remaining components are consistent with Example 1, and the preparation method refers to Example 1.

[0094] Comparative Example 2

[0095] This comparative example provides a mouthwash with antiviral effect. The difference between the mouthwash and Example 1 is that the raw materials of the compound Chinese medicine composition do not include Forsythia suspensa, and the reduced amount of Forsythia suspensa is proportionally distributed to honeysuckle and scutellaria baicalensis, and the other components are consistent with Example 1. The preparation method refers to Example 1.

[0096] Comparative Example 3

[0097] This comparative example provides a mouthwash with antiviral effect. The difference between the mouthwash and Example 1 is that the raw materials of the compound Chinese medicine composition do not include honeysuckle, and the reduced amount of honeysuckle is proportionally distributed to Scutellaria baicalensis and Forsythia suspensa. The remaining components are consistent with Example 1, and the preparation method refers to Example 1.

[0098] Test Example 1 Antibacterial Effect Test

[0099] Preparation of bacterial liquid: (1) For Streptococcus mutans, use MSB solid medium. Take 2 inoculated loops of bacteria and place them in 2 ml of MS liquid medium. Culture them at 37°C for 48 hours. (2) For Staphylococcus aureus, use BP solid medium. Take 2 inoculated loops of bacteria and place them in 2 ml of BP liquid medium. Culture them at 37°C for 48 hours. (3) For Escherichia coli, use BP solid medium. Take 2 inoculated loops of bacteria and place them in 2 ml of BP liquid medium. Culture them at 37°C for 24 hours. (4) For Helicobacter pylori, use BHIA solid medium containing blood brain heart. Take 2 inoculated loops of bacteria and place them in 2 ml of BHI liquid medium. Culture them at 37°C for 72 hours.

[0100] Antibacterial test: The bacterial solution was respectively coated on the surface of the corresponding solid culture medium, and 5 Oxford cups (inner diameter 6 mm, outer diameter 8 mm, height 10 mm) were evenly placed, and 100 μl of the mouthwash and purified water (control) of Examples 1-7 and Comparative Examples 1-3 were added respectively, and cultured under the corresponding culture conditions of each strain, and the diameter of the antibacterial ring around the Oxford cup was measured with a vernier caliper (the diameter was less than 10 mm for the negative control), and the maximum and minimum diameters of the antibacterial ring were measured and the average was taken. Each group of antibacterial tests was repeated 3 times and the average was taken.

[0101] The antibacterial effects of the products prepared in Examples 1-8 and Comparative Examples 1-4 and the control group on different strains are shown in Table 1.

[0102] Table 1

[0103]

[0104]

[0105] It can be seen from the experimental results in Table 1 that the mouthwash provided by Examples 1-3 of the present invention has a more obvious inhibitory effect on oral bacteria; when any one or two of Gastrodia elata or Chuanxiong are missing in Examples 4-6, the antibacterial inhibitory effect of the mouthwash on Streptococcus mutans, Staphylococcus aureus, Helicobacter pylori and Escherichia coli is weakened, indicating that the synergistic cooperation of Gastrodia elata and Chuanxiong in the compound Chinese medicine composition of the present invention has a beneficial effect on the antibacterial effect of the mouthwash; and in Example 7, when honeysuckle, scutellaria, forsythia, Gastrodia elata and Chuanxiong are mixed and decocted during the preparation of the compound Chinese medicine composition, there will be an inhibitory effect between the Chinese medicine components, which will cause the final antibacterial effect of the mouthwash to be poor; in Comparative Examples 1-3, when any one of honeysuckle, forsythia or scutellaria is missing in the composite Chinese medicine composition, the antibacterial effect of the mouthwash is significantly poor, indicating that honeysuckle, scutellaria and forsythia in the compound Chinese medicine composition of the present invention have a synergistic effect in the antibacterial effect of the mouthwash.

[0106] Test Example 2: Anti-virus capability test

[0107] This test example uses avian asymptomatic (weak strain) influenza virus A genus A / swan / Shimane / 499 / 83 (H5N3) of the family Orthomyxovirus as the influenza virus, which is purified by sucrose density gradient centrifugation and dialyzed against PBS to prepare a virus solution for use. The cell line of canine kidney cells, namely MDCK (+) cells, is used for virus proliferation and activity determination. The virus is a weak strain of avian influenza, and the virus solution is purified by sucrose density gradient centrifugation and dialyzed against PBS, and it is believed that there is little mixing of impurities.

[0108] The mouthwash prepared in Examples 1-7 and Comparative Examples 1-3 was diluted to a concentration of 3%, and then mixed with the virus solution at a ratio of 9:1 (10 μL virus solution + 90 μL mouthwash), and reacted at room temperature for 3 minutes. The mixed virus solution was used to prepare a 10-fold stage dilution series with DMEM (the mouthwash concentrations were 0.6%, 0.2%, 0.06%, 0.02%, 0.006%, 0.002%, 0.0006%, 0.0002%, 0.00006%, 0%), and inoculated into the monolayer culture cells of a 96-well plate (50 μL / well) for 1 hour of virus adsorption. The virus inoculum was then removed by aspiration, and DMEM (200 μL / well) and 20 μg / ml trypsin (100 μL / well) were added. After 5 days, the CPE was fixed and stained, and the 50% infection dose was calculated using the Behrens-Kaerber method. The results are shown in Table 2.

[0109] Table 2

[0110]

[0111]

[0112] As shown in Table 2, it can be seen that after the mouthwash prepared in Examples 1-3 of the present invention is diluted with DMEM, the inactivation of viruses is effective at a residual infection titer of 0.002% (20ppm) or more. When the composite Chinese medicine composition described in Examples 4-6 and Comparative Examples 1-3 is not the combination of honeysuckle, scutellaria, forsythia, gastrodia and ligusticum chuanxiong specified in the present invention, the inactivation effect of the mouthwash on viruses is weakened, indicating that the components of the composite Chinese medicine composition in the mouthwash of the present invention cooperate synergistically and have a gain effect; while in Example 7, when all the Chinese medicines are mixed and decocted during the preparation of the composite Chinese medicine composition, there may be an inhibitory effect between the Chinese medicine components, which results in a poor antiviral effect of the final mouthwash.

[0113] Test Example 3: Acute toxicity test in mice

[0114] The mouthwash with antiviral effect prepared in Example 1 was subjected to an acute toxicity test in mice.

[0115] 1. Preparation of test substances

[0116] The mouthwash prepared in Example 1.

[0117] 2. Experimental Animals

[0118] SPF grade Kunming mice, clean grade, weighing 18-22g, half male and half female, a total of 50 mice.

[0119] 3. Dosage grouping: According to Hom's method, four dose groups were set up for female and male mice, including 0 (blank group), 1000, 2150, 4640 and 10000 mg / kg body weight, with 5 animals in each group. The drug was administered orally once, and the exposure dose was calculated as 0.2 ml / 10 g body weight.

[0120] 4. Observation indicators: After exposure, observe the general condition, poisoning symptoms and death of the animals for two weeks. If there is no death within two weeks, the LD can be determined. 50 Greater than 5000 mg / kg body weight; if there is death, the dead mice are dissected to preliminarily determine the cause of death. The experimental results are shown in Table 3.

[0121] Table 3

[0122]

[0123]

[0124] As shown in Table 3, no obvious poisoning reaction was found in any group of animals, and no death was found, indicating that the median lethal dose (acute toxicity) of the antiviral mouthwash of the present invention for female and male mice was LD 50 >5000mg / kg. According to the acute toxicity classification, the mouthwash of the present invention should be a practically non-toxic substance.

[0125] The above test was also carried out on the mouthwashes prepared in Examples 2-7 of the present invention, and similar results were obtained.

[0126] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a mouthwash with antiviral effect and a preparation method thereof, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0127] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0128] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A mouthwash, characterized in that: The components of the mouthwash include, by weight, 5-15 parts of the compound Chinese medicine composition, 1-5 parts of ethanol, 5-10 parts of glycerol, 0.1-1 parts of salt, 0.5-2 parts of peppermint oil, and purified water; The raw materials for preparing the compound Chinese medicine composition are a combination of honeysuckle, forsythia, scutellaria, gastrodia and ligusticum chuanxiong; The mass ratio of honeysuckle, forsythia, scutellaria, gastrodia and ligusticum chuanxiong is (4-5):(1-2):(1-2):(0.1-1):(0.1-1); The compound Chinese medicine composition is prepared by a preparation method comprising the following steps: (1) soaking, boiling, filtering and concentrating each Chinese medicine raw material in purified water to obtain each Chinese medicine concentrate; (2) Mixing and stirring the concentrated Chinese medicine solutions to obtain the compound Chinese medicine composition.

2. The mouthwash according to claim 1, characterized in that The components of the mouthwash include 8-12 parts of the compound Chinese medicine composition, 1-3 parts of ethanol, 7-9 parts of glycerol, 0.1-0.4 parts of salt, 0.8-1.2 parts of peppermint oil and purified water by weight.

3. The mouthwash according to claim 1, characterized in that The soaking time in step (1) is independently 20-40 minutes.

4. The mouthwash according to claim 1, characterized in that The number of decoctions in step (1) is independently 1 to 3 times.

5. The mouthwash according to claim 1, characterized in that The decoction time is independently 1.5-3h.

6. The mouthwash according to claim 1, characterized in that The density of each Chinese medicine concentrated solution concentrated in step (1) is independently 1.05-1.2 g / mL.

7. The mouthwash according to claim 1, characterized in that The mixing and stirring temperature in step (2) is 80-90°C.

8. The mouthwash according to claim 1, characterized in that The mixing and stirring time in step (2) is 10-30 minutes.

9. The mouthwash according to claim 1, characterized in that The components of the mouthwash also include a pH adjuster and / or a preservative.

10. The mouthwash according to claim 9, characterized in that The pH adjuster includes citric acid.

11. The mouthwash according to claim 9, characterized in that The preservatives include sodium benzoate.

12. The method for preparing a mouthwash according to any one of claims 1 to 8, characterized in that: The preparation method comprises: mixing and stirring the compound Chinese medicine composition, ethanol, glycerol, salt and peppermint oil in purified water, and then standing to obtain the mouthwash.

13. The preparation method according to claim 12, characterized in that: The mixing may also include adding a pH adjuster and / or a preservative.

14. The preparation method according to claim 12, characterized in that: The mixing is carried out at a temperature of 22-25°C.

15. The preparation method according to claim 12, characterized in that: The stirring time is 0.5-1.5h.

16. The preparation method according to claim 12, characterized in that: The standing time is 8-12h.

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