Anaerobic microbial metabolism enhancer and preparation method thereof

Abstract

This application relates to the application field of anaerobic microorganisms and specifically discloses an anaerobic microbial metabolism enhancer and its preparation method. The anaerobic microbial metabolism enhancer comprises the following components in percentage by weight: 2%-15% polyaluminium ferric chloride, 10%-50% magnesium salt, 0.1%-5% cobalt salt, 2%-25% potassium salt, 5%-30% buffer, 0.1%-4% trace elements, and 0-27% external additives. The anaerobic microbial metabolism enhancer disclosed herein has the advantage of improving the metabolic efficiency of anaerobic microorganisms.

Description

Technical Field

[0001] The present application relates to the field of anaerobic microbial applications, and more specifically, to an anaerobic microbial metabolism enhancer and a preparation method thereof. Background Art

[0002] The anaerobic biological treatment technology of wastewater is a process that decomposes various complex organic substances in wastewater into substances such as methane and carbon dioxide through the metabolism of anaerobic bacteria and facultative bacteria under anaerobic conditions. It is also called anaerobic digestion. Compared with aerobic biological treatment technology, anaerobic biological treatment technology requires less energy and can generate biogas for resource recycling. The main equipment of the anaerobic biological treatment system is the anaerobic reactor. Anaerobic sludge is the main component of anaerobic digestion in the anaerobic reactor. Anaerobic sludge mainly includes flocculent sludge and granular sludge. The volume load of the anaerobic reactor in the flocculent sludge stage is generally less than 2.5kgCOD / (m 3 d) During the continuous operation of the reactor, anaerobic microorganisms gradually gather together to form tightly structured anaerobic sludge particles, transforming flocculent sludge into granular sludge. Anaerobic granular sludge is a microbial system with self-balancing characteristics that can form physiological and biochemical conditions suitable for bacterial growth, providing a more stable living microenvironment for anaerobic microorganisms. The volume load of the anaerobic reactor in the granular sludge stage is significantly increased, reaching 10kgCOD / (m 3 d) or above.

[0003] However, the high cost of anaerobic reactors contributes to the high investment costs of anaerobic biological treatment technology. To increase the volumetric load of anaerobic reactors, thereby reducing equipment size and investment costs, technologies often enhance the stability and efficiency of anaerobic systems through reactor configuration or improved treatment processes, such as the introduction of microcurrents. However, due to operational limitations and cost constraints, many enhancement methods are difficult to implement in practice.

[0004] Regarding the above-mentioned related technologies, the inventors believe that although the number and activity of anaerobic microorganisms can be increased by adding anaerobic granular sludge, the cost of directly purchasing anaerobic granular sludge is high, and cultivating and debugging anaerobic granular sludge by oneself will have the risk of a long debugging cycle and unsuccessful debugging, so there is room for improvement. Summary of the Invention

[0005] In order to improve the activity and metabolic efficiency of anaerobic microorganisms, the present application provides an anaerobic microorganism metabolism enhancer and a preparation method thereof.

[0006] In the first aspect, the present application provides an anaerobic microbial metabolism enhancer, which adopts the following technical solution:

[0007] An anaerobic microbial metabolism enhancer, comprising the following components in 100% by weight:

[0008] Polyaluminium ferric chloride 2%-15%,

[0009] Magnesium salt 10%-50%,

[0010] Cobalt salt 0.1%-5%,

[0011] Potassium salt 2%-25%,

[0012] Buffer 5%-30%,

[0013] Trace elements 0.1%-4%,

[0014] Added additives 0-27%.

[0015] By adopting the above technical solution, magnesium salt reacts with CO2 generated by anaerobic reaction to generate solid particles MgCO3. The MgCO3 solid particle precipitation can serve as an inert core for forming granular sludge, promoting the formation of granular sludge. Polyaluminum ferric chloride can promote the rapid formation of extracellular polymers. The extracellular polymers gather anaerobic microorganisms together, wrap the inert core, promote the formation of anaerobic sludge particles, greatly shorten the formation time of anaerobic granular sludge, improve the stability of the anaerobic biological system, and help improve the metabolic efficiency of anaerobic microorganisms.

[0016] The metal cations provided by metal salts such as potassium and cobalt provide anaerobic microorganisms with the metal elements required for growth, and are beneficial to improving the electrical conductivity of anaerobic sludge and the electron transfer activity of anaerobic microorganisms, thereby promoting the metabolic efficiency of anaerobic microorganisms; trace elements provide anaerobic microorganisms with the elements necessary for survival, promote the growth and reproduction of anaerobic microorganisms, and are beneficial to further improving the metabolic efficiency of anaerobic microorganisms.

[0017] Preferably, the buffer is sodium bicarbonate.

[0018] Preferably, the trace element includes sodium tungstate.

[0019] By adopting the above technical solution, tungsten can activate the activity of propionate-degrading enzymes of anaerobic microorganisms by regulating the electron transfer of anaerobic microorganisms, promote the degradation of propionic acid in the wastewater treatment process, and thus help improve the wastewater treatment efficiency.

[0020] Preferably, the potency agent further comprises 1%-20% zinc chloride, calculated by mass percentage of the potency agent.

[0021] By adopting the above technical solution, Zn 2+ Can be combined with S in wastewater 2-, the reaction generates ZnS precipitation, inhibits the metabolism of sulfate-reducing bacteria, reduces the formation of sulfide, and reduces S 2- It has a negative impact on the anaerobic microbial system, reduces the generation of hydrogen sulfide gas, reduces the possibility of pH reduction caused by excessive organic load, is beneficial to increase the acid resistance of the anaerobic microbial system, and improves the survival stability of anaerobic microorganisms; at the same time, Zn + It can act as a cofactor, participate in the metabolic reactions of anaerobic microorganisms, and promote the metabolic efficiency of anaerobic microorganisms.

[0022] Preferably, the potency agent further comprises 1%-10% of a chelating agent, calculated based on the mass percentage of the potency agent.

[0023] Preferably, the chelating agent is selected from one or more combinations of EDTA, NTA, and 8-hydroxyquinoline.

[0024] By adopting the above technical solution, the chelating agent can couple with the metal ions released by metal salts such as potassium salts to provide organic matter containing metal elements that is more easily absorbed by anaerobic microorganisms, promote the absorption and utilization of metal ions by anaerobic microorganisms, promote the growth and electron transfer activity of anaerobic microorganisms, and then activate the activity of various enzymes, improve the metabolic efficiency of anaerobic microorganisms, and promote the anaerobic biological treatment rate.

[0025] Preferably, the trace element includes nickel chloride.

[0026] By adopting the above technical solution, cobalt and nickel are essential elements for the F420 coenzyme of anaerobic microorganism methanogens. The combined addition of nickel and cobalt can promote the growth of methanogens and increase the activity of F420 coenzyme. The methanogenesis stage is the rate-limiting stage of anaerobic biological treatment. The increase in the activity of methanogens is conducive to accelerating the methanogenesis rate and improving the efficiency of wastewater treatment.

[0027] Preferably, the potency agent further comprises 1%-20% iron powder and 1%-10% chelating agent in terms of mass percentage of the potency agent.

[0028] By adopting the above technical solution, the iron element provided by the iron powder plays a vital role in the electron transfer system of the F420 coenzyme and is an important component of the F420 coenzyme. The iron element works together with the cobalt element and the nickel element to activate the methanogens, promote the renewal of the methanogens, and promote the gradual transformation of the methanogenic fungi in the reactor into the dominant bacteria species Methanosarcina, greatly improving the reaction efficiency of the methanogenic stage. At the same time, it shortens the adaptation period of the methanogens to the substrate and the sludge acclimation period, which is conducive to increasing the volume load of the anaerobic reactor.

[0029] In addition, iron, cobalt, and nickel have a significant impact on the toxic substance ammonia nitrogen (NH 4+-N) has a significant antagonistic effect, and NH4 + The higher the -N concentration, the more obvious the antagonistic effect of iron, cobalt and nickel on its toxicity, which is beneficial to reduce NH 4+ -N toxic effects on anaerobic microorganisms;

[0030] At the same time, the iron powder participates in the anaerobic microbial reaction to generate Fe 2+ , which can also precipitate S in wastewater 2- , which reduces S 2- It can reduce the negative impact on the anaerobic system, reduce the possibility of pH drop caused by excessive organic load, and help increase the acid resistance of the anaerobic reactor;

[0031] Chelating agents couple with iron ions, cobalt ions, and nickel ions to provide organic matter containing metal elements that are more easily absorbed by anaerobic microorganisms, promote the absorption of iron, cobalt, and nickel by methanogens, and further promote the activation of methanogens.

[0032] Preferably, the iron powder is zero-valent iron powder.

[0033] By adopting the above technical solution, zero-valent iron powder reduces the possibility of methanogens being inhibited, and at the same time can react with pollutants in the water, has strong adsorption properties, can adsorb heavy metals and toxic suspended pollutants in the water, and reduce the toxicity of wastewater.

[0034] Preferably, the external adjuvant comprises one or more combinations of yeast extract 1%-2%, vitamin B2%-3%, calcium salt 4%-8%, redox mediator 2%-4%, and hydrolase 5%-10%, calculated by mass percentage of the strengthening agent.

[0035] Preferably, the redox mediator is selected from one or more combinations of humic acid, lawson, graphene oxide, and anthraquinone.

[0036] By adopting the above technical solution, when the available nutrients in the wastewater are relatively poor, the addition of yeast extract, microorganism B, and calcium salts can provide a more comprehensive nutritional supplement for anaerobic microorganisms, thereby promoting the growth of anaerobic microorganisms; yeast extract can effectively increase the solubility of metal elements such as cobalt and potassium, thereby improving the bioavailability of metal elements; redox mediators can accelerate the transfer of electrons between electron donors and electron acceptors through the cyclic conversion of their own oxidized and reduced states, significantly increasing the rate of biological redox reactions, thereby promoting the anaerobic biotransformation of difficult-to-degrade organic pollutants; yeast extract and redox mediators work together to improve the conductivity of metal cations and the electron transfer activity of anaerobic microorganisms, thereby promoting the rapid degradation of toxic and difficult-to-degrade substances in wastewater;

[0037] When the content of macromolecular organic matter in wastewater is too high, hydrolases are added to assist anaerobic microorganisms in digesting the organic matter. Hydrolases convert macromolecular organic matter into small molecules that are more easily digested by anaerobic microorganisms, thereby promoting the metabolic efficiency of anaerobic microorganisms.

[0038] By adding different types of additional additives, the anaerobic microbial metabolism enhancer can be better applied to the treatment of different types of wastewater such as pharmaceutical wastewater and food wastewater, thereby expanding the scope of application of the anaerobic microbial metabolism enhancer and improving its effect.

[0039] Preferably, based on the mass percentage of the potency agent, the potency agent further comprises 10%-20% of excipients, 7%-14% of binders, and 3%-6% of disintegrants.

[0040] Preferably, the excipients include 5%-15% glucose and 2%-10% starch based on the mass percentage of the fortifying agent.

[0041] Preferably, the adhesive comprises, in terms of mass percentage of the strengthening agent, 5%-10% of polyvinyl alcohol resin and 2%-4% of starch slurry.

[0042] Preferably, the disintegrant comprises, based on the mass percentage of the strengthening agent, 2%-4% dry starch and 1%-2% sodium carboxymethyl starch.

[0043] Through the above technical solution, the preparation process of the strong-enemy agent into a solid-state strong-enemy agent tablet is participated in. The excipient can increase the volume of the strong-enemy agent, the binder binds the various components of the strong-enemy agent together, and the excipient and binder make the strong-enemy agent easy to shape; the disintegrant can make the strong-enemy agent absorb water and swell when entering the wastewater, thereby increasing the solubility of the strong-enemy agent, facilitating the disintegration and release of each component, and facilitating the absorption and utilization of anaerobic microorganisms.

[0044] In a second aspect, the present application provides a method for preparing an anaerobic microbial metabolism enhancer, which adopts the following technical solution:

[0045] A method for preparing an anaerobic microbial metabolism enhancer comprises the following steps:

[0046] According to the proportion, the buffer is dissolved in deionized water to prepare a 10-20 wt% buffer solution, and then other components are added and mixed evenly to obtain the anaerobic microbial metabolism enhancer.

[0047] By adopting the above technical solution, a liquid anaerobic microbial growth enhancer is prepared.

[0048] In a third aspect, the present application provides a method for preparing an anaerobic microbial metabolism enhancer, which adopts the following technical solution:

[0049] An anaerobic microbial metabolism enhancer comprises the following steps:

[0050] The components are mixed evenly according to the proportions and pressed into sheets to obtain the anaerobic microbial metabolism enhancer.

[0051] By adopting the above technical solution, solid anaerobic microbial growth enhancer tablets are prepared.

[0052] In summary, this application has the following beneficial effects:

[0053] 1. The magnesium salt in this application works together with polyaluminium ferric chloride. The magnesium carbonate generated by the reaction of magnesium salt and carbon dioxide serves as the inert core of anaerobic sludge. The flocculant promotes the formation of extracellular polymers. The extracellular polymers gather anaerobic microorganisms together, wrap the inert core, and promote the formation of anaerobic sludge particles, greatly improving the stability of the anaerobic biological system and improving the metabolic efficiency of anaerobic microorganisms. The metal cations provided by metal salts such as potassium and cobalt provide the metal elements required for the growth of anaerobic microorganisms, and are beneficial to improving the conductive properties of anaerobic sludge and the electron transfer activity of anaerobic microorganisms, thereby promoting the metabolic efficiency of anaerobic microorganisms. The trace elements provide the elements necessary for the survival of anaerobic microorganisms, promote the growth and reproduction of anaerobic microorganisms, and are beneficial to further improving the metabolic efficiency of anaerobic microorganisms.

[0054] 2. The enhancer prepared according to the preparation method disclosed in this application can effectively promote the metabolic efficiency of anaerobic microorganisms to achieve the effect of increasing the removal of chemical oxygen demand (COD), which is beneficial to accelerate the treatment efficiency of wastewater by the anaerobic reactor and improve the volume load of the anaerobic reactor. DETAILED DESCRIPTION

[0055] The present application is further described in detail below with reference to the embodiments.

[0056] Example

[0057] Example 1

[0058] The anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 3.0 kg buffer, and 0.3 kg trace elements. In this embodiment, the buffer is sodium bicarbonate and the trace element is copper chloride.

[0059] The preparation method thereof comprises:

[0060] The above-mentioned mass of buffer is dissolved in water to prepare a 10 wt% buffer solution, and the above-mentioned mass of each component is added in sequence and mixed evenly to obtain the anaerobic microbial growth enhancer.

[0061] Example 2

[0062] The only difference between this embodiment and embodiment 1 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 0.2 kg polyaluminum ferric chloride, 5.0 kg magnesium chloride, 0.4 kg cobalt chloride, 1.8 kg potassium chloride, 2.5 kg buffer, and 0.1 kg trace elements.

[0063] Example 3

[0064] The only difference between this embodiment and embodiment 1 is that the trace element is sodium selenate.

[0065] Example 4

[0066] The only difference between this embodiment and embodiment 1 is that the trace element is sodium tungstate.

[0067] Example 5

[0068] The only difference between this embodiment and embodiment 4 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 2.95 kg buffer, 0.3 kg sodium tungstate, and 0.05 kg zinc chloride.

[0069] Example 6

[0070] The only difference between this embodiment and embodiment 5 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 2.0 kg buffer, 0.3 kg sodium tungstate, and 1.0 kg zinc chloride.

[0071] Example 7

[0072] The only difference between this embodiment and embodiment 5 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 0.5 kg buffer, 0.3 kg sodium tungstate, and 2.5 kg zinc chloride.

[0073] Example 8

[0074] The only difference between this embodiment and embodiment 4 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 2.9 kg buffer, 0.3 kg sodium tungstate, and 0.1 kg chelating agent.

[0075] In this embodiment, the chelating agent is NTA.

[0076] Example 9

[0077] The only difference between this embodiment and embodiment 8 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 2.5 kg buffer, 0.3 kg sodium tungstate, and 0.5 kg NTA.

[0078] Example 10

[0079] The only difference between this embodiment and embodiment 8 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 2.0 kg buffer, 0.3 kg sodium tungstate, and 1.0 kg NTA.

[0080] Example 11

[0081] The only difference between this embodiment and embodiment 8 is that the chelating agent is EDTA.

[0082] Example 12

[0083] The only difference between this embodiment and embodiment 1 is that the trace element is nickel chloride.

[0084] Example 13

[0085] The only difference between this embodiment and embodiment 12 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 2.4 kg buffer, 0.3 kg nickel chloride, 0.5 kg chelating agent, and 0.1 kg iron powder.

[0086] In this embodiment, the chelating agent is NTA, and the iron powder is zero-valent iron powder.

[0087] Example 14

[0088] The only difference between this embodiment and embodiment 13 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 1.5 kg buffer, 0.3 kg nickel chloride, 0.5 kg NTA, and 1.0 kg zero-valent iron powder.

[0089] Example 15

[0090] The only difference between this embodiment and embodiment 13 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 0.5 kg buffer, 0.3 kg nickel chloride, 0.5 kg NTA, and 2.0 kg zero-valent iron powder.

[0091] Example 16

[0092] The only difference between this embodiment and embodiment 13 is that the iron powder is magnetite powder.

[0093] Example 17

[0094] The only difference between this embodiment and embodiment 13 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 1.9 kg buffer, 0.3 kg nickel chloride, 0.1 kg NTA, and 1.0 kg zero-valent iron powder.

[0095] Example 18

[0096] The only difference between this embodiment and embodiment 13 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 1.0 kg buffer, 0.3 kg nickel chloride, 1.0 kg NTA, and 1.0 kg zero-valent iron powder.

[0097] Example 19

[0098] The only difference between this embodiment and embodiment 1 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg of polyaluminum ferric chloride, 2.8 kg of magnesium chloride, 0.2 kg of cobalt chloride, 2.5 kg of potassium chloride, 1.9 kg of buffer, 0.3 kg of copper chloride, 0.1 kg of yeast extract powder, 0.2 kg of vitamin B, and 0.8 kg of calcium chloride.

[0099] Example 20

[0100] The only difference between this embodiment and embodiment 19 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg of polyaluminum ferric chloride, 2.8 kg of magnesium chloride, 0.2 kg of cobalt chloride, 2.5 kg of potassium chloride, 2.1 kg of buffer, 0.3 kg of copper chloride, 0.2 kg of yeast extract powder, 0.3 kg of vitamin B, and 0.4 kg of calcium chloride.

[0101] Example 21

[0102] The only difference between this embodiment and embodiment 1 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 2.8 kg buffer, 0.3 kg copper chloride, and 0.2 kg redox mediator.

[0103] In this embodiment, the redox mediator is graphene oxide.

[0104] Example 22

[0105] The only difference between this embodiment and embodiment 21 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 2.6 kg buffer, 0.3 kg copper chloride, and 0.4 kg redox mediator.

[0106] Example 23

[0107] The only difference between this example and Example 21 is that the redox mediator is anthraquinone.

[0108] Example 24

[0109] The only difference between this embodiment and embodiment 19 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg of polyaluminum ferric chloride, 2.8 kg of magnesium chloride, 0.2 kg of cobalt chloride, 2.5 kg of potassium chloride, 1.7 kg of buffer, 0.3 kg of copper chloride, 0.1 kg of yeast extract powder, 0.2 kg of vitamin B, 0.8 kg of calcium chloride, and 0.2 kg of redox mediator.

[0110] Example 25

[0111] The only difference between this embodiment and embodiment 1 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 2.5 kg buffer, 0.3 kg copper chloride, and 0.5 kg hydrolase.

[0112] In this embodiment, the hydrolase is a mixture of equal amounts of protease and amylase.

[0113] Example 26

[0114] The only difference between this embodiment and embodiment 25 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 2.0 kg buffer, 0.3 kg copper chloride, and 1.0 kg hydrolase.

[0115] Example 27

[0116] The only difference between this embodiment and embodiment 1 is that the anaerobic microbial metabolism enhancer includes the following components:

[0117] 1.2kg polyaluminium ferric chloride, 2.8kg magnesium chloride, 0.2kg cobalt chloride, 2.5kg potassium chloride, 0.5kg buffer, 0.3kg copper chloride, 1kg excipient, 1kg binder, 0.5kg disintegrant.

[0118] In this embodiment, the excipients include 0.8 kg glucose and 0.2 kg starch, the binder includes 0.7 kg polyvinyl alcohol resin and 0.3 kg starch slurry, and the disintegrant includes 0.3 kg dry starch and 0.2 kg carboxymethyl starch.

[0119] The preparation method of the anaerobic microbial metabolism enhancer comprises:

[0120] The components of the above mass are mixed evenly, and the mixed powder is put into a tablet press to obtain 2.5 g / tablet anaerobic microbial metabolism enhancer tablets.

[0121] Example 28

[0122] The only difference between this embodiment and embodiment 27 is that the anaerobic microbial metabolism enhancer comprises the following components:

[0123] 1.5 kg polyaluminium ferric chloride, 1.5 kg magnesium chloride, 0.01 kg cobalt chloride, 0.2 kg potassium chloride, 2.5 kg buffer, 0.29 kg copper chloride, 2 kg excipients, 1.4 kg binder, 0.6 kg disintegrant;

[0124] In this embodiment, the excipients include 1.2 kg of glucose and 0.8 kg of starch, the binder includes 1.0 kg of polyvinyl alcohol resin and 0.4 kg of starch slurry, and the disintegrant includes 0.4 kg of dry starch and 0.2 kg of carboxymethyl starch.

[0125] Example 29

[0126] The only difference between this embodiment and embodiment 27 is that the anaerobic microbial metabolism enhancer comprises the following components:

[0127] 1.4 kg polyaluminium ferric chloride, 1.0 kg magnesium chloride, 0.19 kg cobalt chloride, 2.0 kg potassium chloride, 2.9 kg buffer, 0.01 kg copper chloride, 1.5 kg excipients, 0.7 kg binder, 0.3 kg disintegrant;

[0128] In this embodiment, the excipients include 0.5 kg of glucose and 1.0 kg of starch, the binder includes 0.5 kg of polyvinyl alcohol resin and 0.2 kg of starch slurry, and the disintegrant includes 0.2 kg of dry starch and 0.1 kg of carboxymethyl starch.

[0129] Comparative Example

[0130] Comparative Example 1

[0131] The only difference between this comparative example and Example 1 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 2.8 kg magnesium chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 3.0 kg buffer, and 0.3 kg copper chloride.

[0132] Comparative Example 2

[0133] The only difference between this comparative example and Example 1 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 1.2 kg polyaluminum ferric chloride, 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 5.8 kg buffer, and 0.3 kg copper chloride.

[0134] Comparative Example 3

[0135] The only difference between this comparative example and Example 1 is that the anaerobic microbial metabolism enhancer includes the following components by mass: 0.2 kg cobalt chloride, 2.5 kg potassium chloride, 7.0 kg buffer, and 0.3 kg copper chloride.

[0136] Performance test test 1: According to the mass ratio of anaerobic sludge: anaerobic sludge = 3:100, the anaerobic sludge agent or anaerobic sludge agent tablets prepared in Examples 1-29 and Comparative Examples 1-3 were injected or placed into an IC reactor with flocculent sludge, and pharmaceutical wastewater was injected into the pharmaceutical wastewater from the water inlet, so that the reactor performed anaerobic biological treatment on the pharmaceutical wastewater, and the volume load of each reactor was monitored; the pharmaceutical wastewater was directly treated in the IC reactor with flocculent sludge without adding the anaerobic sludge agent, which was recorded as a blank control, and the volume load of the blank control reactor was tested;

[0137] Test 2: According to the mass ratio of anaerobic sludge: anaerobic sludge = 3:100, the anaerobic sludge agent or anaerobic sludge agent tablets prepared in Examples 1-29 and Comparative Examples 1-3 were injected or placed into an IC reactor with flocculent sludge. Food wastewater was then injected from the water inlet to allow the reactor to perform anaerobic biological treatment on the food wastewater, and the volumetric load of each reactor was monitored; the IC reactor with flocculent sludge was used to directly treat food wastewater without adding the anaerobic sludge agent, which was recorded as a blank control, and the volumetric load of the reactor in the blank control group was tested.

[0138] The results are summarized in Table 1:

[0139] Table 1

[0140]

[0141]

[0142] Combining Examples 1-2, Examples 27-29 and the blank group with Table 1, it can be seen that when the anaerobic microbial metabolism enhancer is added to the anaerobic reactor, the volume load of the anaerobic reactor is significantly increased, and both the liquid enhancer medicament and the solid enhancer tablet can function; this indicates that the enhancer prepared according to the formula disclosed in this application can improve the metabolic efficiency of anaerobic microorganisms and promote the increase in the volume load of the anaerobic reactor. This may be because magnesium salts can react with carbon dioxide to form magnesium carbonate precipitates, which can serve as the inert core of anaerobic sludge particles and promote the formation of anaerobic sludge particles; polyaluminum ferric chloride can promote the formation of extracellular polymers, further promote the formation of anaerobic sludge particles, improve the stability of the anaerobic microbial system, and improve the metabolic efficiency of anaerobic microorganisms; metal cations provided by metal salts such as potassium and cobalt provide anaerobic microorganisms with the metal elements required for growth, and are beneficial to improving the electrical conductivity of anaerobic sludge and the electron transfer activity of anaerobic microorganisms, thereby promoting the metabolic efficiency of anaerobic microorganisms; trace elements provide anaerobic microorganisms with the elements necessary for survival, promote the growth and reproduction of anaerobic microorganisms, and further improve the metabolic efficiency of anaerobic microorganisms.

[0143] In combination with Example 1, Comparative Examples 1-3 and Table 1, it can be seen that magnesium salts and polyaluminum ferric chloride have a synergistic effect. Polyaluminum ferric chloride promotes the formation of extracellular polymers. Extracellular polymers gather anaerobic microorganisms together and gradually wrap the inert core of magnesium carbonate, significantly shortening the time for anaerobic sludge particle formation, improving the stability of the anaerobic microbial system, and thus improving metabolic efficiency.

[0144] Combining Example 1, Example 3, Example 4 and Table 1, it can be seen that after adding sodium tungstate, the volume load of the anaerobic reactor is better. This may be because tungstate ions can activate the activity of propionate degrading enzymes of anaerobic microorganisms by regulating the electron transfer of anaerobic microorganisms, thereby promoting the degradation of propionic acid in the wastewater treatment process, thereby helping to improve the wastewater treatment efficiency.

[0145] Combining Example 4, Example 5-7 and Table 1, it can be seen that after adding zinc, the volume load of the anaerobic reactor is further improved. This may be because zinc ions can act as a cofactor and work together with tungstate ions to promote the improvement of the enzyme activity of anaerobic microorganisms, thereby improving the metabolic efficiency of anaerobic microorganisms. At the same time, zinc ions can reduce the free S in wastewater. 2-, inhibiting the metabolism of sulfate-reducing bacteria, reducing the generation of sulfide, and reducing the possibility of pH reduction caused by excessive organic load, which is beneficial to increase the acid resistance of the anaerobic microbial system, improve the survival stability of anaerobic microorganisms, and then promote the production and reproduction of anaerobic microorganisms, and further improve the wastewater treatment efficiency.

[0146] In conjunction with Examples 4, 8-10, and Table 1, it can be seen that the addition of the chelating agent NTA improves the volumetric load of the anaerobic reactor. This may be because NTA can complex with metal ions, promote the absorption and utilization of metal ions by anaerobic microorganisms, promote the growth and electron transfer activity of anaerobic microorganisms, and thereby activate the activity of various enzymes. It works together with tungstate ions to promote the degradation of propionic acid, improve the metabolic efficiency of anaerobic microorganisms, and promote the anaerobic biological treatment rate. In conjunction with Example 11, it is shown that the use of EDTA as a chelating agent can also increase the volumetric load of the anaerobic reactor.

[0147] Combining Example 1, Example 3 and Example 12 with Table 1, it can be seen that the increase in the volumetric load of the anaerobic reactor obtained by selecting nickel chloride is more obvious. This may be because the nickel element and the cobalt element cooperate to promote the activity of the F420 coenzyme of the methanogen, increase the methanogen production rate of the methanogen, and thus improve the wastewater treatment efficiency.

[0148] In combination with Example 12, Examples 13-15, Examples 17-18 and Table 1, it can be seen that after adding zero-valent iron powder and chelating agent NTA, the volume load of the anaerobic reactor is further improved. This may be because the iron element can work together with cobalt and nickel elements to activate and promote the renewal and iteration of methanogens, prompting the methanogenic fungi in the reactor to gradually transform into the dominant bacteria species Methanosarcina, greatly improving the reaction efficiency of the methanogenic stage, while shortening the adaptation period of methanogens to the substrate and shortening the sludge acclimation period, which is beneficial to the increase of the volume load of the anaerobic reactor, and NTA is beneficial to promoting the absorption of iron, cobalt and nickel by methanogens, which is further beneficial to the activation of methanogens; at the same time, iron, cobalt and nickel elements have a great effect on the toxic substance ammonia nitrogen (NH4) in the wastewater. 4+ -N) has a significant antagonistic effect, which is beneficial to reducing the toxic effect of NH4+-N on anaerobic microorganisms.

[0149] Combining Example 13 and Example 16 with Table 1, it can be seen that compared with magnetite powder, zero-valent iron is better for improving volumetric load. This may be because magnetite powder (Fe3O4) is an electron acceptor, which can compete with methanogens for electrons, thereby inhibiting methanogenesis and failing to fully exert the role of the Fe element.

[0150] Combining Examples 19-20, Examples 25-26, and Table 1, it can be seen that the enhancing agent added with yeast extract powder, vitamin B, and calcium salt is more suitable for treating pharmaceutical wastewater, while the enhancing agent added with hydrolase is more suitable for treating food wastewater. This may be due to the different organic matter contents in the wastewater, which also affect the growth and metabolism of anaerobic microorganisms. By adjusting the type and amount of the added additive according to the type of wastewater, the enhancing agent can be adapted to treat different types of wastewater.

[0151] Combining Examples 21-22 with Table 1, it can be seen that adding graphene oxide as a redox mediator can also further increase the volumetric loading of the anaerobic reactor. This is likely due to the redox mediator's ability to mediate and accelerate electron transfer, thereby promoting the redox reaction efficiency of anaerobic microorganisms. In conjunction with Example 23, the volumetric loading of anthraquinone as the redox mediator is superior.

[0152] In combination with Example 19, Example 21, and Example 24 and Table 1, yeast extract can effectively increase the solubility of metal elements and improve the effectiveness of metal elements. The redox mediator cooperates to improve the electrical conductivity of anaerobic sludge, further accelerate the redox reaction, and improve the metabolic efficiency of anaerobic microorganisms.

[0153] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. An anaerobic microbial metabolism enhancer, characterized in that: The invention comprises the following components in percentage by weight: 2%-15% of polyaluminium ferric chloride, 10%-50% of magnesium salt, 0.1%-5% of cobalt salt, 2%-25% of potassium salt, 5%-30% of buffer, 0.1%-4% of trace elements, 0-27% of external additives, 1%-20% of zinc chloride, 1%-10% of chelating agent and 1%-20% of iron powder, wherein the iron powder is zero-valent iron powder, the trace elements include sodium tungstate and nickel chloride, and the external additives include one or more of yeast extract powder 1%-2%, vitamin B 2%-3%, calcium salt 4%-8%, redox mediator 2%-4% and hydrolase 5%-10%.

2. The anaerobic microbial metabolism enhancer according to claim 1, characterized in that: Calculated by weight percentage of the potency agent, the potency agent further includes 10%-20% of an excipient, 7%-14% of a binder, and 3%-6% of a disintegrant.

3. A method for preparing the anaerobic microbial metabolism enhancer according to claim 1, characterized in that: According to the proportion, the buffer is dissolved in deionized water to prepare a 10-20wt% buffer solution, and then other components are added and mixed evenly to obtain the anaerobic microbial metabolism enhancer.

4. A method for preparing the anaerobic microbial metabolism enhancer according to any one of claims 1 to 2, characterized in that: The components are mixed evenly according to the proportions and pressed into sheets to obtain the anaerobic microbial metabolism enhancer.

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