A natural antibacterial composition and its application

Abstract

The present application discloses a natural antibacterial composition, comprising the following components in mass percentages: 0.2% - 2% of Berberis vulgaris root extract, 0.2% - 2% of Magnolia officinalis bark extract, 0.2% - 2% of Ferula assafoetida root extract, 0.2% - 2% of Eleutherococcus senticosus root extract, and the balance being pure water. The present application also discloses the application of the natural antibacterial composition and an essential oil composition emulsion containing the natural antibacterial composition. The components used in the natural antibacterial composition of the present application are mainly natural extracts. These extracts cooperate with each other synergistically, having antibacterial effects, antioxidant properties, and aromatic properties, and can be used as preservatives and antioxidants for daily chemical products including skin care products, washing and care products, aromatherapy, essential oils, etc., with high safety performance.

Description

Technical Field

[0001] The present invention relates to an antibacterial composition, and more particularly to a natural antibacterial composition and its application. Background Art

[0002] So-called preservatives, simply speaking, refer to a class of substances that can inhibit the growth of microorganisms and have a good inhibitory effect on microorganisms that metabolize spoilage substances. Preservatives play a key role in cosmetics, which is closely related to the quality of cosmetics and the safety of consumers during use. The value of preservative application is mainly reflected in reducing the pollution of cosmetics and inhibiting the growth of microorganisms, etc., and can also extend the service life of products. In recent years, the cosmetics market has also changed greatly. The development of the cosmetics market has also driven changes in the use of preservatives. In cosmetics, the role of preservatives is mainly to combine with microbial cells in cosmetics, completely adsorb on the cells, and penetrate into the cytoplasm to effectively inhibit the continuous division of cells, thereby controlling the reproduction rate of microorganisms, avoiding the contamination of cosmetics by bacteria, extending the service life of cosmetics, and ensuring the safety of cosmetics.

[0003] In order to ensure that the application of preservatives in cosmetics can play an effective role, certain requirements also need to be met during the addition of preservatives. The most important thing is to control the concentration and dosage of preservatives. Since preservatives themselves carry certain irritations, if the usage amount is unreasonable, it will not only affect the quality of cosmetics, but may even pose a threat to the skin of users and cause allergies and other situations. With the country putting forward relatively clear requirements for the application of preservatives in cosmetics and people paying more and more attention to health, the selection of cosmetics preservatives has become more and more cautious. Commonly used traditional preservatives in cosmetics include formaldehyde releasers, parabens, alcohols, organic acids, etc. These preservatives have obvious antibacterial effects, but are more irritating to the skin, prone to cause allergic and other adverse reactions, and have a single function and can only be used as antibacterial and preservative components. Summary of the Invention

[0004] In order to overcome the deficiencies of the prior art, the purpose of this application is to provide a natural antibacterial composition derived from plant extracts. Through the cooperation of various active ingredients, it can effectively inhibit bacteria while also having antioxidant and aromatic properties, and can be used as an additive in daily chemical products.

[0005] To solve the above problems, the technical solutions adopted in this application are as follows:

[0006] A natural antibacterial composition, comprising the following components in mass percentage:

[0007] 0.2% - 2% of Berberis vulgaris root extract, 0.2% - 2% of Magnolia officinalis bark extract, 0.2% - 2% of Ferula assafoetida root extract, 0.2% - 2% of Acanthopanax senticosus root extract, and the balance is pure water.

[0008] As a further embodiment, the natural antibacterial composition described in the present application further comprises the following components in mass percentage: 2% - 10% of Thymus vulgaris oil, 20% - 40% of Lavandula angustifolia oil, 2% - 10% of Pelargonium graveolens oil, and 20% - 40% of Citrus aurantium amara flower oil.

[0009] As a further embodiment, the natural antibacterial composition described in the present application further comprises 26% - 55.2% of caprylyl glycol in mass percentage.

[0010] The present application also provides the use of the natural antibacterial composition described in the present application in the preparation of an essential oil emulsion.

[0011] The present application also provides an essential oil composition emulsion, comprising an oil phase and an aqueous phase.

[0012] Wherein the oil phase consists of the following components in mass percentage:

[0013]

[0014]

[0015] The aqueous phase consists of the following components in mass percentage:

[0016]

[0017] The balance is pure water.

[0018] The present application also provides a preparation method of an essential oil composition emulsion, comprising the following steps:

[0019] Mix the components in the oil phase according to the ratio and heat to 70 - 80 °C, stir well to obtain mixture I; mix pure water, disodium EDTA, glycerol, propylene glycol, carbomer, and sodium hyaluronate in the aqueous phase and heat to 70 - 80 °C, stir well to obtain mixture II.

[0020] Mix the above mixture I and mixture II, stir and homogenize at 6000 - 7500 rpm / s for 3 - 5 minutes, and control the stirring process at 70 - 80 °C to obtain mixture III.

[0021] Cool the above mixture III to 58 - 62 °C, add triethanolamine according to the formula amount, stir evenly at low to medium speed, perform vacuum defoaming and then continue stirring to obtain mixture IV.

[0022] Cool down the above mixture Ⅳ to 40 - 47°C, add β-glucan according to the formula amount, and stir evenly at low to medium speed for 10 - 18 min to obtain mixture Ⅴ;

[0023] Cool down the above mixture Ⅴ to 37 - 42°C, add the natural antibacterial combination according to the formula amount, and stir evenly at low to medium speed for 12 - 18 min to obtain the essential oil emulsion.

[0024] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0025] 1. The components used in the natural antibacterial composition described in the present application are mainly natural extracts. These extracts cooperate with each other synergistically, having antibacterial effects, antioxidant properties, and aromatic properties. They can be used as preservatives and antioxidants for daily chemical products including skin care products, washing and care products, aromatherapy, essential oils, etc., with high safety performance.

[0026] 2. The natural antibacterial composition described in the present application has strong antibacterial effects on Escherichia coli and Staphylococcus aureus.

[0027] The following further elaborates on the present invention in conjunction with specific embodiments. Specific Embodiments

[0028] To make the objectives, technical solutions, and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of them. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present application.

[0029] The terms "including", "comprising", and other equivalent descriptive ways involved in the specification and claims of the present application are all intended to cover non-exclusive inclusion, that is, they include both the content clearly described in the specification and claims and can also include steps or units inherent in the product, method, or structure but not described in the specification and claims.

[0030] A natural antibacterial composition includes the following components in mass percentage:

[0031] European barberry root extract 0.2% - 2%, Magnolia officinalis bark extract 0.2% - 2%, Ferula root extract 0.2% - 2%, Acanthopanax senticosus root extract 0.2% - 2%, and the balance is pure water.

[0032] As a further solution, the natural antibacterial composition described in the present application further comprises the following components in mass percentage: 2% - 10% of thymol oil, 20% - 40% of lavender oil, 2% - 10% of geranium oil, and 20% - 40% of neroli oil

[0033] As a further solution, the natural antibacterial composition described in the present application further comprises 26% - 55.2% of caprylyl glycol in mass percentage.

[0034] In the present application, there is a synergistic effect among the components. Thymol, carvacrol, and p - cymene in thymol oil have inhibitory effects on Escherichia coli and Staphylococcus aureus. Neroli oil has strong inhibitory effects on Staphylococcus aureus and Candida albicans. Lavender essential oil contains linalool, limonene, caryophyllene, etc., and has strong inhibitory effects on Escherichia coli. Geranium oil contains citronellol, geraniol, vanillin formate, etc., and has strong antibacterial effects on Escherichia coli and Staphylococcus aureus. Plant extracts such as Berberis vulgaris root extract, Magnolia officinalis bark extract, Ferula assafoetida root extract, and Eleutherococcus senticosus root extract mainly contain anthraquinones and polyphenols, which interfere with the respiratory chain system related to microbial oxidation - reduction and have effective antibacterial and inhibitory effects.

[0035] The present application also provides the use of the natural antibacterial composition described in the present application in the preparation of an essential oil emulsion.

[0036] The present application also provides an essential oil composition emulsion, which comprises an oil phase and an aqueous phase,

[0037] wherein the oil phase is composed of the following components in mass percentage:

[0038]

[0039] The aqueous phase is composed of the following components in mass percentage:

[0040]

[0041] The balance is pure water.

[0042] The present application also provides a preparation method of an essential oil composition emulsion, which comprises the following steps:

[0043] Mix the components in the oil phase according to the ratio and heat to 70 - 80°C, stir well to obtain mixture I; mix pure water, disodium EDTA, glycerol, propylene glycol, carbomer, and sodium hyaluronate in the aqueous phase and heat to 70 - 80°C, stir well to obtain mixture II;

[0044] Mix the above - mentioned mixture I and mixture II, stir and homogenize at 6000 - 7500 rpm / s for 3 - 5 minutes, and control the stirring process at 70 - 80°C to obtain mixture III;

[0045] The above mixture III is cooled to 58 - 62 °C, triethanolamine is added according to the formulation amount, and stirred evenly at low to medium speed. After vacuum degassing, stirring is continued to obtain mixture IV;

[0046] The above mixture IV is cooled to 40 - 47 °C, β-glucan is added according to the formulation amount, and stirred evenly at low to medium speed for 10 - 18 min to obtain mixture V;

[0047] The above mixture V is cooled to 37 - 42 °C, a natural antibacterial combination is added according to the formulation amount, and stirred evenly at low to medium speed for 12 - 18 min to obtain an essential oil emulsion.

[0048] In some embodiments of the present application, when preparing the essential oil composition emulsion, the speed of low-speed stirring is 20 - 35 rpm / min, and the speed of medium-speed stirring is 180 - 280 rpm / min.

[0049] The following are specific embodiments of the present application.

[0050] Examples 1 - 5

[0051] In Examples 1 - 5, a natural antibacterial composition is disclosed, and the specific formulation is shown in Table 1 below.

[0052] Table 1: Composition of Examples 1 - 5

[0053] Ingredient (%) Example 1 Example 2 Example 3 Example 4 Example 5 Thyme oil 0 10 10％ 5％ 2％ Lavender oil 0 40 20％ 30％ 20％ Geranium oil 0 10 2％ 5％ 2％ Neroli oil 0 40 20％ 30％ 20％ Berberis vulgaris root extract 1.0％ 0 2.0％ 0.2％ 0.2％ Magnolia officinalis bark extract 0.2％ 0 2.0％ 1.0％ 0.2％ Ferula assafoetida root extract 2.0％ 0 0.2％ 1.0％ 1.0％ Eleutherococcus senticosus root extract 1.0％ 0 0.2％ 0.2％ 0.5％ Caprylyl glycol 0 0 45％ 26％ 53％ Purified water Balance 0 Balance Balance Balance

[0054] Application Examples 1 - 3

[0055] In Application Examples 1 - 3, an essential oil composition emulsion is disclosed, and the components of the emulsion are shown in Table 2 below.

[0056] Table 2: Composition of the essential oil composition emulsion

[0057]

[0058]

[0059] The essential oil composition emulsions of Application Examples 1 - 3 are prepared according to the following method:

[0060] The components in the oil phase are mixed according to the ratio and heated to 75 °C, and stirred evenly to obtain mixture I; the pure water, disodium EDTA, glycerol, propylene glycol, carbomer, and sodium hyaluronate in the water phase are mixed and heated to 70 - 80 °C, and stirred evenly to obtain mixture II;

[0061] After mixing the above mixture I and mixture II, stir and homogenize at 6000 - 7500 rpm / s for 3 - 5 minutes, and control the stirring process at 75 °C to obtain mixture III;

[0062] The above mixture III is cooled to 60°C, triethanolamine is added according to the formula amount, and stirred evenly at low to medium speed. After vacuum degassing, stirring is continued to obtain mixture IV;

[0063] The above mixture IV is cooled to 45°C, β-glucan is added according to the formula amount, and stirred evenly at low to medium speed for 15 min to obtain mixture V;

[0064] The above mixture V is cooled to 40°C, a natural antibacterial combination is added according to the formula amount, and stirred evenly at low to medium speed for 15 min to obtain an essential oil emulsion.

[0065] Performance testing

[0066] 1. Antiseptic challenge test of adding the natural antibacterial composition to the emulsion

[0067] Refer to the microbial challenge experiment of the United States Pharmacopoeia (USP) (USP 40. <51> Antimicrobial Effectiveness Testing) to conduct an antiseptic challenge test on the essential oil composition emulsion described in Application Examples 1-3.

[0068] The specific experimental steps are as follows:

[0069] Initial inoculation bacterial amount 1×10 6 cfu / g(mL)

[0070] Bacteria (USP-51): Initial concentration is 10 6 ~10 7 cfu / ml; After 7 days, the bacterial quantity is reduced by 99.9% compared with the initial (reduced by 3 orders of magnitude); Compared with 7 days, the colonies do not increase after 14 days, and do not increase compared with 14 days after 28 days.

[0071] Initial inoculation fungal amount 1×10 5 cfu / g(mL)

[0072] Fungi (USP-51): Initial concentration is 10 5 ~10 6 cfu / ml; After 7 days, the fungal quantity is reduced by 90% compared with the initial (reduced by 1 order of magnitude); Compared with 7 days, the colonies do not increase after 14 days, and the colonies do not increase compared with 14 days after 28 days.

[0073] Bacterial mixed inoculation:

[0074] Staphylococcus aureus ATCC6538, Pseudomonas aeruginosa ATCC9027, Escherichia coli ATCC8739 Fungal mixed inoculation:

[0075] Candida albicans ATCC10231, Aspergillus niger ATCC16404.

[0076] The experimental results are shown in Table 3 below.

[0077] Table 3: Results of the anti-corrosion challenge test of the essential oil composition emulsion

[0078]

[0079] The experimental data shows that on the 7th day, the colony count was above 1000 cfu / g, and on the 28th day, the bacteria and fungi were less than 10 cfu / g, passing the anti-corrosion challenge of bacteria and fungi.

[0080] 2. Antibacterial test of different concentrations of natural antibacterial combinations

[0081] The antibacterial composition of Example 5 was added at 0.5% and 1% respectively in the emulsion, and the proportions of other components of the emulsion were the same as those in Application Example 3 above, and the anti-corrosion challenge test was carried out.

[0082] The test results are shown in Table 4 below.

[0083] Table 4: Antibacterial test results of different concentrations of natural antibacterial combinations

[0084]

[0085] The experimental data shows that when adding 0.5% or 1% of the antibacterial combination, the colony count was less than 1000 cfu / g on the 7th day. Both could pass the anti-corrosion challenge test on the 28th day.

[0086] 3. Antioxidant test of the natural antibacterial composition:

[0087] The scavenging ability of DPPH was quantitatively analyzed by spectrophotometry to determine the antioxidant effect of the antibacterial composition. Its antioxidant ability is expressed by the scavenging rate. The greater the scavenging rate, the stronger its antioxidant ability. The natural antibacterial combination of Example 5 was formulated into antibacterial combination ethanol solutions with concentrations of 16, 8, 6, 4, and 2 mg / mL. The antibacterial combination ethanol solution was mixed with 0.1 mmol / L DPPH ethanol solution, and after reacting in the dark for 1 h, the absorbance was measured at 517 nm using an ultraviolet spectrophotometer.

[0088] The DPPH radical scavenging rate was calculated according to the following formula:

[0089] DPPH radical scavenging rate (%) = [1 - (A i - A j ) / A0] × 100%

[0090] Where: A0 is the absorbance value of the mixture of ethanol and DPPH solution at 517 nm; Ai is the absorbance value of the mixed solution of the sample solution and the DPPH ethanol solution at 517 nm; A j is the absorbance value of the mixed solution of the sample solution and ethanol at 517 nm. The DPPH radical scavenging rates of the natural antibacterial composition at different concentrations are shown in Table 5.

[0091] Table 5: DPPH radical scavenging rates of the natural antibacterial composition at different concentrations

[0092] Concentration mg / mL 2 4 6 8 16 Clearance rate 10.2％ 16.8％ 26.4％ 38.2％ 68.4％

[0093] The above embodiments are only the preferred embodiments of the present invention, and the scope of protection of the present invention cannot be limited thereby. Any non-substantial changes and substitutions made by those skilled in the art on the basis of the present invention belong to the scope of protection required by the present invention.

Claims

1. A natural antibacterial composition, characterized in that, Comprising the following components by mass percentage: Berberis vulgaris root extract 0.2% - 2%, Magnolia officinalis bark extract 0.2% - 2%, Ferula assafoetida root extract 0.2% - 2%, Acanthopanax senticosus root extract 0.2% - 2%, Thymus vulgaris oil 2% - 10%, Lavandula angustifolia oil 20% - 40%, Pelargonium graveolens oil 2% - 10%, Citrus aurantium amara flower oil 20% - 40%; the balance is pure water.

2. The natural antibacterial composition according to claim 1, wherein It also includes caprylyl glycol 26% - 55.2% by mass percentage.

3. Use of the natural antibacterial composition according to claim 1 or 2 in the preparation of an essential oil emulsion.

4. An essential oil composition emulsion, characterized in that, Comprising an oil phase and a water phase, wherein the oil phase consists of the following components by mass percentage: Cetearyl alcohol 1.2% - 1.6%; Cetearyl glucoside 0.3% - 0.4%; Glyceryl stearate 0.25% - 0.5%; PEG - 100 stearate 0.25% - 0.5%; Triglyceride (ethylhexanoate) 1.0% - 5.0%; Isononyl isononanoate 1.0% - 5%; Polydimethylsiloxane 1.0% - 2.0%; Olive oil 1.0% - 3.0%; Tocopheryl acetate 0.2% - 0.8%; The water phase consists of the following components by mass percentage: Disodium EDTA 0.03% - 0.05%; Glycerol 1.0% - 5.0%; Propylene glycol 1.0% - 5.0%; Carbomer 0.1% - 0.2%; Sodium hyaluronate 0.03% - 0.05%; Triethanolamine 0.08% - 0.15%; β - Glucan 1.0% - 3.0%; The natural antibacterial composition according to claim 1 or 2 0.5% - 1.2%; Pure water in the balance.

5. A method for preparing an essential oil composition emulsion as described in claim 4, characterized in that, Comprising the following steps: Mix the components in the oil phase according to the ratio and heat to 70 - 80°C, stir well to obtain mixture I; mix pure water, disodium EDTA, glycerol, propylene glycol, carbomer, and sodium hyaluronate in the water phase and gradually heat to 70 - 80°C, stir well to obtain mixture II; After mixing the above mixture I and mixture II, stir and homogenize at 6000 - 7500 rpm / s for 3 - 5 minutes, and control the stirring process at 70 - 80°C to obtain mixture III; Cool the above mixture III to 58 - 62°C, add triethanolamine according to the formula amount, stir evenly from low speed to medium speed, and continue to stir after vacuum degassing to obtain mixture IV; Cool the above mixture IV to 40 - 47°C, add β - glucan according to the formula amount, stir evenly from low speed to medium speed for 10 - 18 min to obtain mixture V; Cool the above mixture V to 37 - 42°C, add the natural antibacterial combination according to the formula amount, stir evenly from low speed to medium speed for 12 - 18 min to obtain the essential oil emulsion.

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