A large-scale cherry seedling rapid shaping growth cultivation method

Abstract

The application discloses a large-scale Chinese cherry seedling rapid shaping growth cultivation method, which comprises the following steps: S101, seedling selection and planting hole digging; S102, planting: single-hole double-planting V-shaped planting is carried out; S103, soil covering and pre-promotion management; S104, middle-promotion management I: when new shoots grow to 10 cm, amidoxydiphenylmethane is sprayed on leaves; S105, middle-promotion management II: amino acid water-soluble fertilizer is sprayed on leaves; and S106, daily management. The application has the beneficial effects that: the application realizes the dense planting cultivation for tourism development by means of single-hole double-planting V-shaped planting during the Chinese cherry seedling period, inoculation of Leptomitus fragilans agent through pre-promotion management during the seedling period, spraying of amidoxydiphenylmethane through middle-promotion management I and additional spraying of amino acid water-soluble fertilizer through middle-promotion management II, the seedling period of the Chinese cherry is shortened, and the requirement of rapid fruit setting is met.

Description

Technical Field

[0001] This invention relates to the field of agricultural planting technology, and in particular to a method for large-scale, rapid, and controlled growth cultivation of Chinese cherry seedlings. Background Technology

[0002] In recent years, with the development of the market economy and the improvement of people's consumption levels and changes in dietary structure, the structure of fruit production has also changed, with small fruits becoming increasingly popular, and the development of Chinese cherries experiencing a surge. However, "cherries are delicious, but the trees are difficult to cultivate." Current Chinese cherry cultivation faces two main problems: first, the orchard has a long juvenile period, requiring 4-5 years to reach full production; second, the trees have excessively large canopies after maturity, making harvesting and management difficult, severely restricting the development of the cherry industry. Meanwhile, with the development of the tourism economy, the spacious areas within orchards can be utilized for tourism, such as catering, rest, parking, and fruit picking, improving the utilization rate of the orchard's vertical space. However, due to existing cultivation problems, it is difficult to shape the trees for tourism development after they have reached maturity, making it difficult to form a large-scale, intensive planting model for tourism development. Furthermore, current Chinese cherry cultivation mostly relies on post-maturity fertilization and watering management, resulting in a long cycle from seedling to mature tree, often requiring 1-2 years to reach maturity. Chinese cherry seedlings grow relatively slowly. Summary of the Invention

[0003] The purpose of this invention is to overcome the shortcomings of the prior art and provide a method for large-scale rapid shaping and cultivation of Chinese cherry seedlings, which can complete intensive planting for tourism development when the Chinese cherry seedlings are still young, while shortening the juvenile stage of Chinese cherries and enabling them to bear fruit quickly.

[0004] The objective of this invention is achieved through the following technical solution: a method for large-scale rapid shaping and cultivation of Chinese cherry seedlings, comprising the following steps:

[0005] S101. Seedling selection and planting hole digging: Select one-year-old Chinese cherry seedlings with plump buds as planting seedlings. The seedlings should have intact taproots, no less than 3 lateral roots, and a height of 60cm or more. At the same time, ridges should be made in the planting area and planting holes for dense planting should be dug on the ridges.

[0006] S102. Planting: During the suitable planting period, plant two seedlings in a single hole in a "V" shape. Place the seedlings with a length difference of less than 5% in pairs in the planting hole. The roots of the two seedlings are 20-40 cm apart and arranged in a V shape. The main stems of the two seedlings face each other and are oriented towards the row to complete the seedling planting and shaping.

[0007] S103. Covering with soil and pre-planting management: Spread the roots of the seedling in the planting hole and straighten it. Then fill the planting hole with fine soil, lifting the seedling while filling the soil until the roots of the seedling are at 1 / 3 of the depth of the planting hole. Spread the brittle sessile fungicide evenly on the surface of the fine soil. Continue to fill the fine soil until the planting hole is 2 / 3 of the depth. Spread the brittle sessile fungicide evenly on the surface of the fine soil. Continue to fill the fine soil until the soil depth is level with the original soil mark at the root of the seedling. Water thoroughly to settle the roots. Cover the ridge with black mulch to retain moisture and ensure that the main stem leaves 2-3 buds.

[0008] S104, Mid-term Management I: After the seedlings sprout in spring, retain 5-8 buds from the top downwards and remove all the remaining buds at the bottom. When the new shoots grow to 10cm, spray the leaves with amino acid ester at a concentration of 20-40 mg / L.

[0009] S105, Medium-term management II: 3-5 days after spraying amino acid fresh oil, spray the leaves with an amino acid water-soluble fertilizer diluted 600-1500 times;

[0010] S106. Daily Management: When the primary branches of the seedlings reach 50-90 cm in length and the secondary and tertiary branches reach 30-40 cm in length, daily fertilizer and water management should be carried out to complete the shaping and cultivation of the seedlings and enter the management of mature seedlings.

[0011] To address the challenges of tall, uncontrollable canopies, vigorous vegetative growth, and late fruiting in Chinese cherry trees, production typically employs artificial control, chemical regulation, and root restriction methods. Currently, in southern regions, plant growth regulators are primarily used to suppress tree growth and achieve dwarfing. However, improper and excessive use leads to harmful residues and premature aging of the trees. Root restriction cultivation is beneficial for controlling the height and polar growth of fruit trees, thus promoting flower bud formation. Studies on root restriction cultivation in Chinese cherries have shown significant effects in controlling above-ground vegetative growth, increasing yield, and improving fruit quality. However, implementing root restriction cultivation requires laying root restrictors or lining the planting holes with non-woven fabric, increasing orchard establishment costs and creating obstacles for later water and fertilizer management, making large-scale promotion in production difficult.

[0012] Therefore, this application proposes a double-plant "V"-shaped cultivation model for Chinese cherry, achieving the effect of root-limited cultivation through root interactions. Orchard ecosystems are not isolated entities; they form populations or communities at different scales, making intraspecific and interspecific interactions inevitable. Intraspecific root interactions (interspecific interactions) manifest as the efficient resource acquisition by crop populations of different densities; interactions between roots in an interaction system can be categorized as competition and mutualism. The spatial distribution of root responses to neighboring plants in an interaction system is characterized by avoidance, tolerance, and invasion. Avoidance manifests as plants reducing root growth near neighboring plants, leading to spatial niche separation. Niche separation refers to the phenomenon where related species in the same domain choose niches to reduce competition for resources. Vertical root niche separation drives the efficient utilization of nutrient resources in different soil layers. Horizontal niche separation is an important mechanism for plants to avoid competition.

[0013] This application utilizes the niche separation caused by interspecific root interactions to achieve root zone restriction cultivation. Two Chinese cherry trees are planted in the same hole, 30 cm apart, with their main trunks facing each other towards the row, forming a V-shape at a 45° angle to the ground. This encourages root growth to extend outwards towards the row. Combined with annual trenching and root pruning and fertilization between the rows, and the use of mulch for water control, root growth can be effectively controlled, thereby controlling tree vigor and promoting earlier fruiting.

[0014] The seedlings mentioned in step S101 are grafted seedlings, and the thickness at 1 cm above the graft union is more than 0.6 cm.

[0015] The planting density in step S101 is a plant spacing of 2-2.5 meters and a row spacing of 3-3.5 meters.

[0016] In step S102, the seedlings that have completed planting and shaping are at a 45° angle to the ground.

[0017] Before transplanting, the process also includes root treatment, which involves pruning the root suckers, broken branches and roots of the seedlings, shortening roots longer than 30cm to no more than 30cm, soaking the seedlings in water for 12-16 hours before transplanting, and soaking the roots in a mud slurry of 600 times diluted carbendazim and 1000 times diluted ABT rooting powder for 10-12 minutes to promote root development.

[0018] The planting hole is 30cm in diameter and 30cm in depth.

[0019] Mycorrhizae are symbiotic relationships formed between plant roots and fungi, and are ubiquitous in nature. Among them, arbuscular mycorrhizal fungi (AMF) form the most widespread symbiotic relationships with plants and are a very important class of functional microorganisms in soil. AMF have multiple functions, including enhancing plant absorption of mineral elements from the soil and improving plant growth. The AMF strains used in this application were purchased from the "Arbuscular Mycorrhizal Fungi Germplasm Resource Bank" of the Institute of Plant Nutrition and Resources, Beijing Academy of Agricultural and Forestry Sciences, namely *Gastromycotomyces moses* (resource bank number BJ01), *Gastromycotomyces brittle* (resource bank number BJ02B), *Gastromycotomyces cryptotachys* (resource bank number BJ04B), and *Gastromycotomyces juvenile* (resource bank number XJ04B). Following existing methods, the fungi were propagated using clover, with the culture medium consisting of river sand and zeolite in a 6:4 ratio, and irrigated with phosphorus-free Hogrange nutrient solution. The propagated fungi were then dried and stored at 4°C. The clover culture medium was autoclaved at 0.11 MPa and 121°C for 2 hours before use. The cultured and propagated inoculum was dried and stored at 4°C. The dosage of the *Streptococcus brittleus* inoculum was 150-200 g per planting hole, with the same dosage applied at 1 / 3 and 2 / 3 depth of the planting hole.

[0020] The following is a comparative experiment conducted by the applicant to verify the effects of the sessile schizocarp fungicide on the growth of Chinese cherry seedlings:

[0021] In the first comparative experiment, sterilized soil was filled into plastic flowerpots 20 cm high and 21 cm in diameter. 50 g of inoculant was applied at 1 / 3 and 2 / 3 of the depth from the bottom of the pot, totaling 100 g per pot. Five treatments were administered: no inoculant (control), inoculated with *Gnaphalium mossense*, inoculated with *Gnaphalium brittleense*, inoculated with *Gnaphalium cryptogams*, and inoculated with *Gnaphalium juvenileense*. Each treatment was replicated three times, for a total of 15 pots. An equal amount of sterilized river sand and zeolite mixture was added to the control. Seedlings of uniform growth (approximately 10 cm tall) were then transplanted into the pots, with four seedlings planted per pot, distributed along the four sides of the pot's edge. The pots were placed randomly, with a 10 cm interval between pots, and the pots were periodically rotated during seedling growth to mitigate marginal effects. Watering was provided regularly during seedling growth to maintain soil field capacity above 80%. Seedlings were harvested two months after planting.

[0022] At seedling harvest, the fourth mature leaf from the top was used to determine the content of photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids); the fifth mature leaf from the top was used to determine the activities of antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as the content of soluble protein; and the sixth mature leaf from the top was used to determine the activities of selenium metabolism-related enzymes ATP sulfonase (ATPS), APS reductase (APR), serine acetyltransferase (SAT), and selenocysteine ​​methyltransferase (SMT). Photosynthetic pigment content was determined using the acetone-ethanol (1:1) extraction method. SOD activity, POD activity, CAT activity, and soluble protein content were determined using the nitroblue tetrazolium (NBT) method, guaiacol colorimetric method, ultraviolet spectrophotometry, and Coomassie Brilliant Blue G-250 method, respectively. Afterward, the entire plant was harvested, rinsed thoroughly with tap water, then repeatedly rinsed with distilled water, and dried with absorbent paper. The peach seedlings were separated into roots, stems, and leaves. They were blanched at 115 ℃ for 15 min, dried at 80 ℃ to constant weight, and their biomass (dry weight) was determined using a 0.1 g electronic balance. The seedlings were then pulverized and passed through a 100-mesh sieve for later use. Data were analyzed using SPSS 20.0 statistical software for variance analysis, and multiple comparisons were performed using Duncan's new multiple range method (P < 0.05).

[0023] The data on the impact of comparative experiment 1 on the biomass (dry weight) of Chinese cherry seedlings are shown in Table 1:

[0024] Table 1: Effects on seedling biomass

[0025]

[0026] Note: Different lowercase letters after the data in the same column in the table indicate that the differences between treatments reached a significant level (P < 0.05), and the same applies below.

[0027] As shown in Table 1, compared with no application, *Gastropoda fragilis* increased the biomass of seedling roots, leaves, and aboveground parts by 11.28% (P < 0.05), 12.00% (P < 0.05), and 9.18% (P < 0.05), respectively, but had no significant effect on seedling stem biomass (P > 0.05). Compared with no application, *Gastropoda moses*, *Gastropoda cryptotachys*, and *Gastropoda juvenilea* all reduced the biomass of seedling roots, stems, leaves, and aboveground parts to some extent.

[0028] The data on the effect of comparative experiment 1 on the photosynthetic pigment content of Chinese cherry seedlings are shown in Table 2:

[0029] Table 2: Effects on photosynthetic pigment content in seedlings

[0030]

[0031] Table 2 shows that, compared with no application, *Gastromycota moses* and *Gastromycota cryptota* reduced the chlorophyll a content of seedlings, while *Gastromycota brittle* and *Gastromycota juvenile* had no significant effect on the chlorophyll a content of seedlings (P > 0.05). Regarding chlorophyll b content, only *Gastromycota brittle* and *Gastromycota cryptota* increased the chlorophyll b content of peach seedlings; the other treatments had no significant effect on the chlorophyll b content of seedlings (P > 0.05). The effects of the four arbuscular mycorrhizal fungi on the total chlorophyll content of seedlings were not significant (P > 0.05). Regarding carotenoid content, except for *Gastromycota cryptota* which reduced the carotenoid content of seedlings, the other treatments had no significant effect on the carotenoid content of peach seedlings (P > 0.05).

[0032] The data on the effects of comparative experiment 1 on the antioxidant enzyme activity and soluble protein content of Chinese cherry seedlings are shown in Table 3:

[0033] Table 3: Effects on antioxidant enzyme activity and soluble protein content in seedlings

[0034]

[0035] Table 3 shows that, compared with no application, *Aphelenchus brittleus* and *Cryptospira* reduced the SOD activity of seedlings, while *Cryptospira mosesi* and *Cryptospira juvenilea* had no significant effect on the SOD activity of seedlings (P>0.05). All four arbuscular mycorrhizal fungi increased the POD activity of seedlings, in the following order: *Aphelenchus brittleus* > *Cryptospira mosesi* > *Cryptospira* > *Cryptospira juvenilea* > no application. Compared with no application, *Aphelenchus brittleus*, *Cryptospira mosesi*, *Cryptospira*, and *Cryptospira juvenilea* increased the POD activity of seedlings by 91.17% (P<0.05), 40.30% (P<0.05), 28.53% (P<0.05), and 20.60% (P<0.05), respectively.

[0036] Regarding CAT activity, *Gastrodia brittleness*, *Gastrodia moses*, and *Gastrodia cryptota* all increased CAT activity in seedlings, by 85.55% (P < 0.05), 55.75% (P < 0.05), and 44.69% (P < 0.05), respectively, compared to no application. *Gastrodia juvenilee*, however, did not have a significant effect on CAT activity in seedlings (P > 0.05). *Gastrodia moses*, *Gastrodia brittleness*, and *Gastrodia juvenilee* all decreased the soluble protein content in seedlings, while *Gastrodia cryptota* did not have a significant effect on the soluble protein content in seedlings (P > 0.05).

[0037] As can be seen from the first comparative experiment, the schizocarpium brittleense agent increased the biomass (dry weight), photosynthetic pigment content and antioxidant enzyme activity of seedlings to a certain extent compared with other inoculants, which is beneficial to the rapid growth and shaping of Chinese cherry seedlings.

[0038] In step S104, the concentration of DA-6 was 30 mg / L. DA-6, chemically known as diethylaminoethanol hexanoate, is a widely used cytokinin-based plant growth regulator. DA-6 is characterized by enhancing crop stress and disease resistance, improving quality, being biodegradable, non-toxic, having no side effects, and being safe and highly effective. DA-6 can also promote the absorption of nutrients by cherry seedlings.

[0039] The following is a comparative experiment conducted by the applicant to verify the effects of amino acid esters on the growth of Chinese cherry seedlings:

[0040] In the second comparative experiment, in February 2021, cherry seeds were germinated for two weeks using moist perlite. Then, seedlings were raised in high-bottomed trays (50 cells) filled with perlite (2%-6% moisture content), with 1 L of Hoagland's nutrient solution poured into the trays. The nutrient solution was changed every 3 days. The soil was air-dried, crushed, and sieved through a 5 mm sieve. 3.0 kg of the crushed soil was placed in plastic pots (15 cm high, 18 cm in diameter) for later use. In March 2021, cherry seedlings with uniform growth (6-8 true leaves, approximately 6-9 cm tall) were transplanted into pots, with 4 seedlings per pot. Afterwards, the cherry seedlings were sprayed with different concentrations (0, 10, 20, 30, and 40 mg / L) of DA-6 solution, with each treatment repeated 3 times (3 pots). The spraying amount was just enough to cause dripping from the leaf tips. Spraying was done every 7 days for a total of 4 times.

[0041] Samples were collected one month after the first application to determine the content of photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids), antioxidant enzyme activities (SOD, POD, and CAT), soluble protein content, and biomass. Photosynthetic pigment content was determined using the ethanol-acetone extraction method; SOD activity was determined using the NBT photochemical reduction method; POD activity was determined using the guaiacol method; CAT activity was determined using the potassium permanganate titration method; and soluble protein content was determined using the Coomassie brilliant blue colorimetric method. Whole cherry seedlings were harvested, washed with tap water, and then rinsed three times with distilled water. The harvested cherry seedlings were separated into roots, stems, and leaves. They were blanched at 105℃ for 15 min, dried at 75℃ to constant weight, and the biomass (dry weight) of each part was weighed using an electronic balance. Aboveground biomass = stem biomass + leaf biomass. Data were analyzed using SPSS (Duncan's new multiple range method for multiple comparisons) and Pearson correlation analysis.

[0042] The data on the impact of comparative experiment 2 on the biomass (dry weight) of Chinese cherry seedlings are shown in Table 4:

[0043] Table 4: Effects of DA-6 on seedling biomass

[0044]

[0045] Table 4 shows that the biomass of cherry seedling roots, stems, leaves, and aboveground parts were all higher than the control after treatment with different concentrations of DA-6, and showed a trend of first increasing and then decreasing with increasing DA-6 concentration. The biomass of cherry seedling roots, leaves, and aboveground parts was significantly higher than the control at a DA-6 concentration of 30 mg / L, increasing by 24.34% (P<0.05), 18.95% (P<0.05), and 23.28% (P<0.05), respectively, while the other treatments showed no significant difference from the control (P≥0.05). The stem biomass treatments, ranked from highest to lowest, were: 30 mg / L > 40 mg / L > 20 mg / L > 10 mg / L > control, reaching its maximum at a DA-6 concentration of 30 mg / L, increasing by 30.35% compared to the control (P<0.05).

[0046] The data on the effect of comparative experiment 2 on the photosynthetic pigment content of Chinese cherry seedlings are shown in Table 5:

[0047] Table 5: Effects of DA-6 on photosynthetic pigment content in cherry seedlings

[0048]

[0049] Table 5 shows that, after treatment with different concentrations of DA-6, except for carotenoid content, the contents of chlorophyll a, chlorophyll b, and total chlorophyll all showed a trend of first increasing and then decreasing with increasing DA-6 concentration. The contents of chlorophyll a, chlorophyll b, and total chlorophyll were highest at a DA-6 concentration of 30 mg / L, increasing by 12.61% (P<0.05), 32.32% (P<0.05), and 17.75% (P<0.05) respectively compared to the control. The changes in chlorophyll a and total chlorophyll contents were consistent, with the treatments ranked from highest to lowest as follows: 30 mg / L > 40 mg / L > 20 mg / L > 10 mg / L > control. The chlorophyll b content, ranked from highest to lowest, was: 30 mg / L > 20 mg / L > 40 mg / L > 10 mg / L > control. There was no obvious pattern in the changes of carotenoid content.

[0050] The data on the effects of comparative experiment 2 on the antioxidant enzyme activity and soluble protein content of Chinese cherry seedlings are shown in Table 6:

[0051] Table 6: Effects of DA-6 on antioxidant enzyme activity and soluble protein content in cherry seedlings

[0052]

[0053] Table 6 shows that the activities of the three antioxidant enzymes in cherry seedlings changed similarly after spraying with different concentrations of DA-6. The activities first increased and then decreased with increasing DA-6 concentration, and all activities were higher than the control. At a DA-6 concentration of 30 mg / L, the activities of SOD, POD, and CAT were the highest, increasing by 9.46% (P<0.05), 41.53% (P<0.05), and 214.77% (P<0.05) respectively compared to the control. The activities were second highest at a DA-6 concentration of 40 mg / L, increasing by 5.73% (P<0.05), 38.81% (P<0.05), and 138.59% (P<0.05) respectively compared to the control. The soluble protein content of cherry seedlings was higher than that of the control. It showed a trend of first increasing and then decreasing with the increase of DA-6 concentration. The maximum value was reached when the DA-6 concentration was 30 mg / L, which was 10.75% higher than the control (P<0.05). The next highest value was reached when the DA-6 concentration was 40 mg / L, which was 6.61% higher than the control (P<0.05). There were no significant differences among the other treatments (P≥0.05).

[0054] Meanwhile, in order to verify the effect of amino acid esters on nutrient absorption in Chinese cherry seedlings, the applicant conducted a comparative experiment three:

[0055] Using the same experimental materials and procedures as in Comparative Experiment 2, samples were collected one month after the first spraying. Whole plants were harvested, rinsed thoroughly with tap water, and then rinsed three times repeatedly with deionized water, before being dried with absorbent paper. Cherry seedlings were separated into roots, stems, and leaves. These were blanched in an oven at 105℃ for 15 min, then dried at 75℃ to constant weight, pulverized, and passed through a 0.149 mm sieve. 0.2 g each of the dried, pulverized, and sieved cherry seedling roots, stems, and leaves were weighed into an Erlenmeyer flask, 5 mL of H2SO4 was added, shaken well, covered with a film, and left overnight. The mixture was then digested with sulfuric acid-hydrogen peroxide on a hot plate until colorless and clear. The solution was filtered and diluted to 50 mL for the determination of total nitrogen, total phosphorus, and total potassium content. Total nitrogen content was determined using the Kjeldahl method, total phosphorus content using the molybdenum-antimony colorimetric method, and total potassium content using a flame photometer. The data were analyzed using SPSS 16.0 software (Duncan's new multiple range method was used for multiple comparisons).

[0056] The data on the impact of comparative experiment 3 on the total nitrogen content of Chinese cherry seedlings are shown in Table 7:

[0057] Table 7: Effects of DA-6 on total nitrogen content in cherry seedlings

[0058]

[0059] As shown in Table 7, after spraying different concentrations of DA-6, the total nitrogen content of cherry seedling roots, stems, leaves and aboveground parts increased significantly compared with the control. Moreover, the total nitrogen content increased first and then decreased with the increase of DA-6 concentration. The treatments were ordered from highest to lowest as follows: 30 mg / L > 40 mg / L > 20 mg / L > 10 mg / L > 0 mg / L. The total nitrogen content in the roots, stems, leaves, and aboveground parts of cherry seedlings reached its maximum at a DA-6 concentration of 30 mg / L, increasing by 77.36% (P<0.05), 220.52% (P<0.05), 48.14% (P<0.05), and 77.49% (P<0.05) respectively compared to the control. The next highest concentration was at DA-6 concentration of 40 mg / L, increasing by 65.42% (P<0.05), 191.18% (P<0.05), 36.05% (P<0.05), and 62.27% (P<0.05) respectively compared to the control.

[0060] The data on the impact of comparative experiment 3 on the total nitrogen content of Chinese cherry seedlings are shown in Table 8:

[0061] Table 8: Effects of DA-6 on total phosphorus content in cherry seedlings

[0062]

[0063] Table 8 shows that the total phosphorus content in different organs of cherry seedlings varied after spraying with different concentrations of DA-6. Within the DA-6 treatment concentration range of 10-40 mg / L, the total phosphorus content in the roots showed a trend of first increasing and then decreasing. Compared with the control, the total phosphorus content in the roots was lower at a DA-6 treatment concentration of 10 mg / L, and higher at the other treatment concentrations. The total phosphorus content in the roots reached its maximum at a DA-6 treatment concentration of 30 mg / L, increasing by 19.94% compared to the control (P<0.05). The total phosphorus content in the stems, leaves, and aboveground parts showed the same trend, all showing a trend of first increasing and then decreasing with increasing DA-6 treatment concentration. The treatments, ranked from highest to lowest, were: 30 mg / L > 40 mg / L > 20 mg / L > 10 mg / L > 0 mg / L. The total phosphorus content in stems, leaves, and aboveground parts reached its maximum when the DA-6 concentration was 30 mg / L, increasing by 49.84% (P<0.05), 18.40% (P<0.05), and 23.85% (P<0.05) respectively compared to the control. The next highest concentration was 40 mg / L, increasing by 43.27% (P<0.05), 16.29% (P<0.05), and 20.46% (P<0.05) respectively compared to the control.

[0064] The data on the impact of comparative experiment 3 on the total nitrogen content of Chinese cherry seedlings are shown in Table 9:

[0065] Table 9: Effects of DA-6 on total potassium content in cherry seedlings

[0066]

[0067] Table 9 shows that the changes in total potassium content in various organs of cherry seedlings after spraying with different concentrations of DA-6 were consistent, showing a trend of first increasing and then decreasing with increasing DA-6 concentration, and all were significantly higher than the control. The total potassium content in roots, stems, leaves, and aboveground parts reached its maximum at a DA-6 concentration of 30 mg / L, increasing by 20.85% (P<0.05), 15.99% (P<0.05), 41.77% (P<0.05), and 34.18% (P<0.05) compared to the control, respectively; the concentration of 40 mg / L was the second highest, increasing by 18.46% (P<0.05), 14.43% (P<0.05), 27.42% (P<0.05), and 22.15% (P<0.05) compared to the control, respectively.

[0068] Chlorophyll is the fundamental substance for photosynthesis in leaves, and its content determines the strength of photosynthesis. Treatment with DA-6 significantly increased the content of chlorophyll a, chlorophyll b, and total chlorophyll in plant leaves. Chlorophyll biosynthesis is a series of complex reactions; changes in any step can alter chlorophyll content. DA-6 can promote the conversion of δ-aminolevulinic acid (ALA) to bile pigmentogen (PBG) and PBG to urogen III during chlorophyll biosynthesis, and significantly enhance the activities of key enzymes promoting these two steps: δ-aminolevulinic acid dehydratase (ALAD) and urogen III synthase (UROS), thereby increasing chlorophyll content. Cytokinin-based plant growth regulators can increase the root surface area of ​​plants, promote root nutrient absorption, and thus regulate plant growth and development. DA-6 is a cytokinin-based plant growth regulator. Studies have shown that DA-6 can promote root growth and increase the biomass of various organs. Comparative experiments two and three showed that spraying different concentrations of DA-6 significantly increased the biomass of all organs in cherry seedlings. Except for carotenoid content, the contents of chlorophyll a, chlorophyll b, and total chlorophyll all increased with increasing DA-6 concentration. The activities of the three antioxidant enzymes also increased with increasing DA-6 concentration (most significantly at 30 mg / L). Simultaneously, DA-6 promoted root growth in cherry seedlings, allowing for better root contact with the soil and thus enabling the absorption of more nutrients. The contents of total nitrogen, phosphorus, and potassium all increased (most significantly at 30 mg / L), and the activity of nitrogen metabolism-related enzymes was enhanced, thereby strengthening the nitrogen metabolism capacity of cherry seedlings.

[0069] The applicant conducted a comparative experiment from April to June 2020 to verify the effect of amino acid water-soluble fertilizer on the growth of Chinese cherry seedlings. The experiment mainly consisted of the following five processes: (1) Seedling cultivation. Perlite was placed in 50-hole high-bottom trays and Hoagland nutrient solution for seedling cultivation (the seeds were moistened with perlite for two weeks in the early stage). The perlite used for seedling cultivation was pumice-like grayish-white perlite with a water content of 2% to 6%. 1 L of nutrient solution was poured into the trays and the nutrient solution was changed every 3 days. The seedlings were cultivated day and night in an artificial climate chamber at 21 ℃ to 23 ℃. The emergence of the radicle from the seed coat was considered germination. After the seeds germinated and emerged, nutrient solution was poured in at the appropriate time. (2) Soil potting. The uncontaminated soil was dried, crushed, and sieved through a 5 mm sieve. 3.0 kg of the soil was weighed and placed in plastic pots of 15 cm × 18 cm (height × diameter). The soil was kept moist and left to stand naturally for 2 weeks for later use. (3) Transplanting of seedlings. When the seedlings have grown to 6-8 true leaves (6-9 cm tall), select seedlings with uniform growth and transplant them into seedling pots. Plant 4 seedlings in each pot (distributed in four directions), for a total of 15 pots. Place the plastic pots (spaced about 15 cm apart) randomly and change their positions randomly from time to time to reduce the influence of edge effect. (4) Seedling spraying. Dilute the amino acid water-soluble fertilizer to 600 times, 900 times, 1200 times and 1500 times respectively, and then spray the seedling leaves (the leaves are covered with water droplets of uniform size). Spray 50 mL per pot, and spray distilled water for CK. There are 5 treatments in total, and each treatment is repeated 3 times (3 pots). Spray once every 15 days, for a total of 2 sprays. When spraying, pay attention to spraying separately to avoid the interaction of concentration. (5) Sampling and harvesting. After 30 days of cultivation, select mature leaves to measure the photosynthetic and resistance physiological indicators of the seedlings, and then harvest the whole plant. Rinse it clean with tap water, and then rinse the roots repeatedly with distilled water. Wipe it dry with absorbent paper. The plant was divided into stems and leaves. Both were blanched at 105 ℃ and dried at 75 ℃ to constant weight. The biomass of each part of the seedling was then weighed. Specifically, the third or fourth mature leaf (0.1 g) from the top of the seedling was collected, and the content of photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoids) was determined. The determination method was an acetone-ethanol (1:1) 10 mL mixed extraction method, with colorimetric measurements at wavelengths of 663 nm, 645 nm, and 470 nm. The contents of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids were calculated, following the method described by Xiong Qing'e. The total chlorophyll content was calculated as the sum of chlorophyll a and chlorophyll b. Simultaneously, 0.2 g of the third or fourth mature leaf (from the top) of a peach seedling was collected and ground in a mortar under ice bath conditions. 2 mL of phosphate buffer (pH 7.8) was added, and the mixture was ground until homogenized. The homogenate was then transferred to a 10 mL centrifuge tube. The mortar was rinsed three times with buffer solution, and the liquid was transferred to the centrifuge tube to a total volume of 6 mL. The tube was centrifuged at 4 ℃ and 6000 rpm for 15 min. The supernatant was the crude enzyme extract and stored at -20 ℃ for later use. The crude enzyme solution was used to determine superoxide dismutase (SOD) activity using the NBT photochemical reduction method, peroxidase (POD) activity using the guaiacol method, catalase (CAT) activity using the potassium permanganate titration method, and soluble protein content using the Coomassie brilliant blue colorimetric method. Data processing was performed using Excel 2010 software, and analysis of variance (Duncan's method) and correlation analysis were conducted using SPSS 26.0 software.

[0070] Data on the impact of comparative experiment four on the biomass (dry weight) of Chinese cherry seedlings are shown in [link to experimental data]. Figure 1 and Figure 2 As shown, from Figure 1 and Figure 2 It was observed that the stem biomass of seedlings treated with amino acid fertilizer at different dilutions was significantly higher than that of the control group. The leaf biomass of seedlings treated with amino acid fertilizer at dilutions of 900, 1200, and 1500 times was significantly different from that of the control group. The 1500-fold dilution of amino acid fertilizer resulted in the highest stem and leaf biomass of seedlings, increasing by 51.28% (P < 0.05) and 35.74% (P < 0.05), respectively. The stem and leaf biomass of peach seedlings increased with increasing dilution, with the following order from lowest to highest: CK < 600 < 900 < 1200 < 1500.

[0071] The data on the effects of comparative experiment four on the photosynthetic pigment content of Chinese cherry seedlings are shown in Table 10:

[0072] Table 10: Effects of Amino Acid Water-Soluble Fertilizer on Photosynthetic Pigment Content in Seedlings

[0073]

[0074] As shown in Table 10, under different dilution ratios of amino acid water-soluble fertilizer, the 1500-fold dilution significantly promoted the total chlorophyll content and relative carotenoid content in seedling leaves, increasing them by 10.94% (P < 0.05) and 8.49% (P < 0.05) respectively compared to the control group. The chlorophyll a, chlorophyll b, and carotenoid contents in other treatment groups did not differ significantly from the control group.

[0075] The data on the effects of comparative experiment four on the antioxidant enzyme activity of Chinese cherry seedlings are shown in Table 11:

[0076] Table 11: Effects of Amino Acid Water-Soluble Fertilizers on Antioxidant Enzyme Activity

[0077]

[0078] Table 11 shows that different dilutions of the amino acid water-soluble fertilizer all increased the activity of antioxidant enzymes in seedlings. Except for the 600-fold dilution, where the SOD activity was not significantly different from the control group, the activities of SOD, POD, and CAT were significantly different from the control group in all other treatments. At a 1500-fold dilution, the activities of the three antioxidant enzymes increased by 32.81% (P < 0.05), 26.93% (P < 0.05), and 60.89% (P < 0.05), respectively. Higher dilutions of the amino acid water-soluble fertilizer resulted in higher antioxidant enzyme activity, with POD activity (CK) < 600 < 900 < 1200 < 1500. The soluble protein content at 600, 900, and 1200-fold dilutions was not significantly different from the control group, but at a 1500-fold dilution, the soluble protein content was significantly lower than the control group.

[0079] As can be seen from the fourth comparative experiment, the application of amino acid water-soluble fertilizer at different dilution ratios increased the biomass of peach seedling stems and leaves, increased the chlorophyll content of seedling leaves, and improved the activity of antioxidant enzymes. This indicates that amino acid water-soluble fertilizer can promote the growth and development of peach seedlings, with the best effect achieved when diluted 1500 times.

[0080] Based on comparative experiments one through four, the applicant, in this application, uses the effects of *Trichoderma brittleness* fungicide on Chinese cherry seedlings as a factor influencing pre-promoting management. This promotes biomass growth, photosynthetic pigment content, and antioxidant enzyme activity in the seedling stage, facilitating rapid growth and shaping of Chinese cherry seedlings. Following pre-promoting management, intermediate-promoting management I is implemented. DA-6 further promotes biomass growth, photosynthetic pigment content, and antioxidant enzyme activity in the seedlings. Furthermore, DA-6 promotes nutrient absorption, which facilitates the absorption of amino acid water-soluble fertilizer by the Chinese cherry seedlings in intermediate-promoting management II, further promoting biomass growth, photosynthetic pigment content, and antioxidant enzyme activity. Through these treatments, the juvenile stage of Chinese cherries can be significantly shortened, achieving rapid fruit setting.

[0081] The soil pH value of the planting area should be 6.0-7.5. The planting area should be far from pollution sources and have open terrain. A slope of less than 15° is suitable for planting in a north-south direction on flat and gentle slopes; for mountainous and hilly areas with a slope greater than 15°, planting in contour lines is suitable. Furthermore, planting should be avoided in windy areas, valleys where cold air accumulates, and low-lying areas. Continuous cropping is not recommended. Ideally, the planting area should have an average annual temperature above 12℃, an extreme minimum temperature in early March not lower than -2℃, an average annual rainfall of 600mm-1400mm, and 1800-2800 hours of sunshine per year. In addition, the planting area should have an irrigation water source that does not accumulate waterlogged. Irrigation conditions should comply with NY / T5010-2016 (Environmental Conditions for Pollution-Free Agricultural Product Cultivation).

[0082] Meanwhile, to verify the effectiveness of the "V" planting method of two plants per hole, the applicant conducted a comparative experiment five. Starting in 2016, the applicant conducted a comparative experiment of two plants per hole and one plant per hole (simultaneously selecting 50 planting holes, with the average number of branches). The data on the changes in branch quantity over three consecutive years and its impact on flowering of Chinese cherry trees were recorded, as detailed in Tables XII and XIII.

[0083] Table 12: Effects of single-hole two-plant cultivation on the number of Chinese cherry branches

[0084]

[0085] Table 13: The effect of single-hole two-plant cultivation on flowering of Chinese cherry

[0086]

[0087] It is evident that single-hole double-plant cultivation results in higher branch density and more uniform plant growth compared to conventional planting methods. The branches required for high yields develop earlier, with trial fruiting occurring in the second year after planting, and yields achieved in the third year. Once mature, the plant can maintain a high branch density. In contrast, single-hole single-plant cultivation requires four years after planting to begin achieving yields.

[0088] Five years after planting, the yields of Chinese cherries cultivated using the two methods differed significantly. The yield per tree in the "two trees per hole" method was 6.14 kg, lower than the 10.73 kg yield per tree in the "one tree per hole" method. However, due to the higher planting density (222 trees per mu), the yield per mu reached 1423.02 kg. The standing harvest rate also differed significantly between the two methods. Because the "two trees per hole" method allowed for slanted growth, the tree height could be controlled to around 2 meters, resulting in a standing harvest rate of 83.77%, effectively reducing labor. Currently, this technology also reduces labor input and pesticide costs for integrated pest management, saving over 500 yuan in labor and over 120 yuan in pesticide costs per mu (see Table 14 for details).

[0089] Table 14: Yield and Standing Harvest Quantity of 5-Year-Old Chinese Cherry under Different Growing Patterns

[0090]

[0091] The beneficial effects of this invention are as follows: This application achieves intensive planting for tourism development of Chinese cherry seedlings by planting two plants in a single hole in a "V" shape during the seedling stage, and simultaneously inoculating the seedlings with a sclerotium brittleii fungicide during the pre-promoting management stage, spraying 20-40 mg / L of amino acid esters during the mid-promoting management stage I, and adding amino acid water-soluble fertilizer diluted 600-1500 times during the mid-promoting management stage II, thus achieving the goal of rapid fruit setting.

[0092] Instruction manual illustrations

[0093] Figure 1 This is a schematic diagram comparing the stem biomass of Chinese cherry seedlings treated with amino acid water-soluble fertilizer in Comparative Example 4 of this invention.

[0094] Figure 2 This is a schematic diagram comparing the biomass of stems and leaves of Chinese cherry seedlings treated with amino acid water-soluble fertilizer in Comparative Example 4 of this invention.

[0095] Figure 3 This illustrates the "V"-shaped planting configuration of two plants per hole according to the present invention. Detailed Implementation

[0096] The technical solution of the present invention will be further described in detail below with reference to the embodiments, but the scope of protection of the present invention is not limited to the following description.

[0097] Example 1

[0098] A method for rapid and standardized cultivation of Chinese cherry seedlings, comprising the following steps:

[0099] S101. Seedling selection and planting hole digging: Select one-year-old Chinese cherry seedlings with plump buds as planting seedlings. The seedlings should have intact taproots, no less than 3 lateral roots, and a height of 60cm or more. At the same time, ridges should be made in the planting area and planting holes for dense planting should be dug on the ridges.

[0100] S102. Planting: During the suitable planting period, plant two seedlings in a single hole in a "V" shape. Place the seedlings with a length difference of less than 5% in pairs in the planting hole. The roots of the two seedlings are 30 cm apart and arranged in a V shape. The main stems of the two seedlings face each other and are oriented towards the row to complete the seedling planting and shaping.

[0101] S103. Covering with soil and pre-planting management: Spread the roots of the seedling in the planting hole and straighten it. Then fill the planting hole with fine soil, lifting the seedling while filling the soil until the roots of the seedling are at 1 / 3 of the depth of the planting hole. Spread the brittle sessile fungicide evenly on the surface of the fine soil. Continue to fill the fine soil until the planting hole is 2 / 3 of the depth. Spread the brittle sessile fungicide evenly on the surface of the fine soil. Continue to fill the fine soil until the soil depth is level with the original soil mark at the root of the seedling. Water thoroughly to settle the roots. Cover the ridge with black mulch to retain moisture and ensure that the main stem leaves 2-3 buds.

[0102] S104, Mid-term Management I: After the seedlings sprout in spring, retain 5-8 buds from the top downwards and remove all the remaining buds at the bottom. When the new shoots grow to 10cm, spray the leaves with 30 mg / L of amino acid oil.

[0103] S105, Mid-term Management II: Four days after spraying amine fresh oil, spray the leaves with an amino acid water-soluble fertilizer diluted 1500 times;

[0104] S106. Daily Management: When the primary branches of the seedlings reach 50-90 cm in length and the secondary and tertiary branches reach 30-40 cm in length, daily fertilizer and water management should be carried out to complete the shaping and cultivation of the seedlings and enter the management of mature seedlings.

[0105] Example 2

[0106] A method for rapid and standardized cultivation of Chinese cherry seedlings, comprising the following steps:

[0107] S101. Seedling selection and planting hole digging: Select one-year-old Chinese cherry seedlings with plump buds as planting seedlings. The seedlings should have intact taproots, no less than 3 lateral roots, and a height of 60cm or more. At the same time, ridges should be made in the planting area and planting holes for dense planting should be dug on the ridges.

[0108] S102. Planting: During the suitable planting period, plant two seedlings in a single hole in a "V" shape. Place the seedlings with a length difference of less than 5% in pairs in the planting hole. The roots of the two seedlings are 20 cm apart and arranged in a V shape. The main stems of the two seedlings face each other and are oriented towards the row to complete the seedling planting and shaping.

[0109] S103. Covering with soil and pre-planting management: Spread the roots of the seedling in the planting hole and straighten it. Then fill the planting hole with fine soil, lifting the seedling while filling the soil until the roots of the seedling are at 1 / 3 of the depth of the planting hole. Spread the brittle sessile fungicide evenly on the surface of the fine soil. Continue to fill the fine soil until the planting hole is 2 / 3 of the depth. Spread the brittle sessile fungicide evenly on the surface of the fine soil. Continue to fill the fine soil until the soil depth is level with the original soil mark at the root of the seedling. Water thoroughly to settle the roots. Cover the ridge with black mulch to retain moisture and ensure that the main stem leaves 2-3 buds.

[0110] S104, Mid-term Management I: After the seedlings sprout in spring, retain 5-8 buds from the top downwards and remove all the remaining buds at the bottom. When the new shoots grow to 10cm, spray the leaves with 20 mg / L of amino acid oil.

[0111] S105, Mid-term Management II: Four days after spraying amine fresh oil, spray the leaves with an amino acid water-soluble fertilizer diluted 600 times;

[0112] S106. Daily Management: When the primary branches of the seedlings reach 50-90 cm in length and the secondary and tertiary branches reach 30-40 cm in length, daily fertilizer and water management should be carried out to complete the shaping and cultivation of the seedlings and enter the management of mature seedlings.

[0113] Example 3

[0114] A method for rapid and standardized cultivation of Chinese cherry seedlings, comprising the following steps:

[0115] S101. Seedling selection and planting hole digging: Select one-year-old Chinese cherry seedlings with plump buds as planting seedlings. The seedlings should have intact taproots, no less than 3 lateral roots, and a height of 60cm or more. At the same time, ridges should be made in the planting area and planting holes for dense planting should be dug on the ridges.

[0116] S102. Planting: During the suitable planting period, plant two seedlings in a single hole in a "V" shape. Place the seedlings with a length difference of less than 5% in pairs in the planting hole. The roots of the two seedlings are 40 cm apart and arranged in a V shape. The main stems of the two seedlings face each other and are oriented towards the row to complete the seedling planting and shaping.

[0117] S103. Covering with soil and pre-planting management: Spread the roots of the seedling in the planting hole and straighten it. Then fill the planting hole with fine soil, lifting the seedling while filling the soil until the roots of the seedling are at 1 / 3 of the depth of the planting hole. Spread the brittle sessile fungicide evenly on the surface of the fine soil. Continue to fill the fine soil until the planting hole is 2 / 3 of the depth. Spread the brittle sessile fungicide evenly on the surface of the fine soil. Continue to fill the fine soil until the soil depth is level with the original soil mark at the root of the seedling. Water thoroughly to settle the roots. Cover the ridge with black mulch to retain moisture and ensure that the main stem leaves 2-3 buds.

[0118] S104, Mid-term Management I: After the seedlings sprout in spring, retain 5-8 buds from the top downwards and remove all the remaining buds at the bottom. When the new shoots grow to 10cm, spray the leaves with 40 mg / L of amino acid oil.

[0119] S105, Mid-term Management II: Three days after spraying amine fresh oil, spray the leaves with an amino acid water-soluble fertilizer diluted 900 times;

[0120] S106. Daily Management: When the primary branches of the seedlings reach 50-90 cm in length and the secondary and tertiary branches reach 30-40 cm in length, daily fertilizer and water management should be carried out to complete the shaping and cultivation of the seedlings and enter the management of mature seedlings.

[0121] In the fifth comparative experiment, 20 planting holes (average value) were selected in the same planting area for each example. Simultaneously, existing methods for cultivating Chinese cherry seedlings (i.e., single-hole, single-plant, un-shaped seedling fertilization treatment) were used as Comparative Example 1, and single-hole, double-plant, un-shaped seedling fertilization treatment was used as Comparative Example 2. Sampling was conducted simultaneously one month after the fertilization treatment. Specific sampling procedures can be found in Comparative Experiments 1 to 4. The comparison results are as follows:

[0122] The data on the impact of comparative experiment 5 on the biomass (dry weight) of Chinese cherry seedlings are shown in Table 15:

[0123] Table 15: Effects on seedling biomass

[0124]

[0125] As can be seen from Table 15, the biomass of Chinese cherry seedlings in Examples 1-3 showed a more significant increase compared to Comparative Examples 1 and 2.

[0126] The above description is merely a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the forms disclosed herein and should not be construed as excluding other embodiments. It can be used in various other combinations, modifications, and environments, and can be altered within the scope of the concept described herein through the above teachings or related technologies or knowledge. Modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention should be within the protection scope of the appended claims.

Claims

1. A method for large-scale, rapid, and standardized cultivation of Chinese cherry seedlings, characterized in that, It includes the following steps: S101. Seedling selection and planting hole digging: Select one-year-old Chinese cherry seedlings with plump buds as planting seedlings. The seedlings should have intact taproots, no less than 3 lateral roots, and a height of 60cm or more. At the same time, ridges should be made in the planting area and planting holes for dense planting should be dug on the ridges. S102. Planting: During the suitable planting period, plant two seedlings in a single hole in a "V" shape. Place the seedlings with a length difference of less than 5% in pairs in the planting hole. The roots of the two seedlings are 20-40 cm apart and arranged in a V shape. The main stems of the two seedlings face each other towards the row to complete the seedling planting and shaping. S103. Covering with soil and pre-planting management: Spread out the seedling roots in the planting hole and straighten the seedling. Then fill the planting hole with fine soil while lifting the seedling until the seedling roots are at 1 / 3 of the depth of the planting hole. Spread the brittle sessile fungicide evenly on the surface of the fine soil. Continue to fill the fine soil until the planting hole is 2 / 3 of the depth. Spread the brittle sessile fungicide evenly on the surface of the fine soil. Continue to fill the fine soil until the soil depth is level with the original soil mark at the root of the seedling. Water thoroughly to settle the roots. Cover the ridge with black mulch to retain moisture. Ensure that the main stem retains 2-3 buds. The dosage of the brittle sessile fungicide is 150-200g per hole, with the same dosage at 1 / 3 and 2 / 3 of the depth of the planting hole. S104, Mid-term Management I: After the seedlings sprout in spring, retain 5-8 buds from the top downwards and remove all the remaining buds at the bottom. When the new shoots grow to 10cm, spray the leaves with 30mg / L of amino acid oil. S105, Medium-term management II: 3-5 days after spraying amino acid fresh oil, spray the leaves with an amino acid water-soluble fertilizer diluted 600-1500 times; S106. Daily Management: When the primary branches of the seedlings reach 50-90 cm in length and the secondary and tertiary branches reach 30-40 cm in length, daily fertilizer and water management should be carried out to complete the shaping and cultivation of the seedlings and enter the management of mature seedlings.

2. The method for rapid shaping and cultivation of Chinese cherry seedlings on a large scale according to claim 1, characterized in that, The seedlings mentioned in step S101 are grafted seedlings, and the thickness at 1 cm above the graft union is more than 0.6 cm.

3. The method for rapid shaping and cultivation of Chinese cherry seedlings on a large scale according to claim 1, characterized in that, The planting density in step S101 is a plant spacing of 2-2.5 meters and a row spacing of 3-3.5 meters.

4. The method for rapid shaping and cultivation of Chinese cherry seedlings on a large scale according to claim 1, characterized in that, In step S102, the seedlings that have completed planting and shaping are at a 45° angle to the ground.

5. The method for rapid shaping and cultivation of Chinese cherry seedlings on a large scale according to claim 1, characterized in that, Before transplanting, the process also includes root treatment, which involves pruning the root suckers, broken branches, and broken roots of the seedlings. Roots longer than 30cm are shortened to no more than 30cm. Before transplanting, the seedlings are soaked in water for 12-16 hours and then soaked in a mud slurry of 600 times diluted carbendazim and 1000 times diluted ABT rooting powder for 10-12 minutes to promote root development.

6. A method for rapid shaping and cultivation of Chinese cherry seedlings on a large scale according to any one of claims 1 or 3, characterized in that, The planting hole is 30cm in diameter and 30cm in depth.

7. The method for rapid shaping and cultivation of Chinese cherry seedlings on a large scale according to claim 1, characterized in that, The soil pH value in the planting area is 6.0-7.5.

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