A centrifugal liquid layer separation system and a separation method thereof

Abstract

The present application relates to the technical field of medical liquid products, and provides a centrifugal liquid layer separation system, which comprises a liquid flow module, a self-contrast recognition module for obtaining the layering condition of the liquid flowing through the liquid flow module, an automatic separation module connected with the liquid flow module and used for controlling the on-off state of the liquid flow, and a central processing module connected with the self-contrast recognition module and the automatic separation module respectively. The present application also provides a separation method of the centrifugal liquid layer separation system. The present application solves the problems that the separation process of the layered liquid is affected by manual operation and the separation process cannot have unified standards. The present application can automatically separate, reduce the error caused by manual identification, realize standardized separation, has high separation precision, and greatly improves the separation efficiency of the layered liquid.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical liquid products, and more particularly to a centrifugal liquid layer separation system and a separation method thereof. BACKGROUND

[0002] In clinical practice, it is often necessary to centrifugally separate liquid layers such as blood, cell suspension, and exosome-containing solution, and use a certain layer of the separated liquid layer for treatment or experiment. For example, blood-derived regenerative factors are usually formed by centrifuging peripheral blood or bone marrow blood to form a separated liquid layer, and then extracting platelet concentrate containing regenerative factors from the separated blood after centrifugation. Blood-derived regenerative factors can provide various growth factors, promote body repair, promote wound closure, stimulate tissue regeneration, and enhance local anti-infection ability. Numerous studies have shown that regenerative factors can accelerate the repair and regeneration of bone and soft tissue. Blood-derived regenerative factors can be extracted from a patient's own blood, are abundant in source, safe and effective, and are widely used in plastic and cosmetic, oral surgery, orthopedics, and other treatments. For example, exosome-containing solution can be used for in vitro diagnosis, detection, or concentrated treatment after centrifugal separation, which are common and important application scenarios in clinical practice.

[0003] Currently, the separation and preparation of such medical special cell liquid or exosome-containing solution is usually manually operated, and most of the extraction devices on the market are also manually operated devices. The technical personnel places the liquid into a centrifuge, which rotates at high speed to separate the liquid into layered liquid components in the bag, and then manually separates the layered liquid layer by layer. The current method, as described above, requires multiple places, multiple manual operations, multiple devices, and multiple auxiliary consumables. At the same time, the entire separation process is complicated and time-consuming, which will also affect the quality of the layered liquid components.

[0004] Since the separation process of blood, cell suspension, and exosome-containing solution is currently manually operated, there is no unified standard for the entire extraction process, which is greatly affected by human factors. The extraction concentration ratio of the same individual and batch varies greatly, which greatly affects the extraction quality of the layered liquid. Therefore, it is necessary to realize the automatic separation and standardized enrichment process of medical layered liquid (such as blood, cell suspension, and exosome-containing solution), and to improve the precision and efficiency of the medical layered liquid separation process. SUMMARY

[0005] The present application aims to solve the problem that medical layered liquid (such as blood, cell suspension, exosome-containing solution, etc.) is affected by manual operation during liquid layer separation and the separation process has no uniform standard, resulting in large fluctuations in the quality of the separated liquid layer, and provides a centrifugal liquid layer separation system and a separation method thereof. The present application can automatically separate the layered liquid, reduce the errors caused by manual identification and separation operation, and greatly improve the separation efficiency of the layered liquid.

[0006] To solve the above technical problems, the technical scheme adopted by the present application is:

[0007] A centrifugal liquid layer separation system, comprising a liquid flow module, a self-contrast identification module for obtaining the layering condition of the liquid flowing through the liquid flow module, an automatic separation module connected to the liquid flow module and controlling the on-off state of the liquid flow, and a central processing module connected to the self-contrast identification module and the automatic separation module, respectively.

[0008] In this way, the layered liquid is transported from the liquid flow module, the self-contrast identification module identifies the layering condition of the flowing liquid, and the identification information is transmitted to the central processing module in real time. When different liquid layer changes are identified, the central processing module sends instructions to the automatic separation module to control the on-off state of the liquid flow, so as to automatically and intelligently separate different liquid layers.

[0009] Further, the liquid flow module comprises a separation site for loading the layered liquid after centrifugation and a liquid receiving site for loading the liquid layer after separation, and a detachable separation tube is arranged on each of the separation site and the liquid receiving site. The separation tubes on the separation site and the liquid receiving site are connected to each other through an identification tube.

[0010] Further, the self-contrast identification module comprises a light source module for irradiating the flowing liquid, and a light identification module for identifying the light absorption value of the flowing liquid, and the light identification module is connected to the central processing module.

[0011] Further, the light source module is one of a constant light source or a combination light source.

[0012] It should be noted that the constant light source is a visible or invisible light with stable output of a specific wavelength, and the combination light source is a light source matrix of at least two specific wavelengths that emits light at the same time or according to a certain algorithm. The constant light source and the combination light source emitted can be absorbed by the layered liquid. The light identification module identifies the absorption value of the layered liquid to the light source, transmits the data to the central processor, and then judges the ratio or concentration of each component in the liquid, thereby realizing the accuracy of separation.

[0013] Thus, the light emitted by the light source module irradiates the flowing liquid, and the light recognition module of the receiving part recognizes itself by comparison with different liquid layers in the same tube. The recognition process does not contact the liquid, and different liquid layers have different light absorption values and frequencies. In this way, the light absorption value of the flowing liquid is recognized, and a real-time receiving and feedback recognition module is formed.

[0014] Further, the central processing module includes a signal processing module and a control module connected to the signal processing module. The signal processing module is connected to the light recognition module, and the control module is connected to the automatic separation module.

[0015] Further, the signal processing module removes individual differences of the flowing liquid by coupling algorithm processing to reduce recognition errors and recognize the layered changes of the liquid.

[0016] In this way, by coupling algorithm processing of light absorption value, the recognition error caused by different light absorption values of individual differences of the liquid is removed, and the purpose of accurately separating the target liquid layer is achieved.

[0017] Further, the centrifugal liquid layer separation system further comprises an identification chamber with an opening and closing door, a flow channel is formed in the identification chamber, and the two ends of the flow channel are respectively connected to the separation site and the liquid receiving site. The identification tube is located in the flow channel, and the light source module and the light recognition module are respectively arranged on the two sides of the flow channel.

[0018] The automatic separation module includes a guide member coaxially arranged with the separation tube on the separation site, a moving seat connected to the guide member, and a driving module for reciprocating movement of the moving seat on the guide member. The driving module is connected to the control module.

[0019] It should be noted that the identification chamber is provided with an opening and closing door. On the one hand, it is convenient for connecting and dismounting the separation tube on the separation site and the liquid receiving site. On the other hand, the identification chamber can be closed during work, so that the identification chamber is in airtight and darkroom state, ensuring that the light recognition module is not affected by external light, and improving the recognition accuracy. It should be further noted that the flow channel and the built-in identification hose can control the flux of the liquid discharged from the separation tube of the separation site, prolong the flow path, and ensure the recognizable area of the recognition module, so as to improve the recognition accuracy.

[0020] In this way, the automatic device is designed to replace manual operation for subsequent separation of the layered liquid, reduce errors caused by manual recognition, perform standardized separation operation, improve the quality stability of the separated liquid layer, and reduce the quality fluctuation of the same batch of products.

[0021] It should be further noted that all modules and structures of the centrifugal liquid layer separation system can be installed on a main body, so that they are integrated into an independent device.

[0022] Further, the separation tube comprises a carrier liquid tube with two open ends, a movable piston arranged in the open end of the carrier liquid tube, and a push rod detachably connected with the piston, and the push rod is detachably connected with the moving seat.

[0023] It should be noted that the detachable separation tube design can realize rapid liquid suction when the layered liquid is loaded by installing the push rod, and the layered liquid in the carrier liquid tube can be centrifuged separately to realize layering after the push rod is detached; when the layered liquid is separated, the carrier liquid tube with the detached push rod is more flexible and convenient to install and detach, and the connection between the detached push rod and the moving seat is also more flexible and convenient, which is convenient and suitable for the separation operation of layered liquid with different volumes in the carrier liquid tube.

[0024] Further, the central processing module further comprises a display and a control panel, and the display and the control panel are connected with the control module.

[0025] It should be noted that the start, stop, control factor concentration, and separation threshold of the separation process automatic separation module can be controlled through the control panel, and the state of the separation of the liquid layer during the separation process of the layered liquid can be directly and intuitively read in real time through the display, and the separation steps and separation parameters.

[0026] The application also provides a separation method of the centrifugal liquid layer separation system, which specifically comprises the following steps:

[0027] S1: The layered liquid is placed in the liquid flow module for transportation, and the liquid layers of the layered liquid are sequentially layered from top to bottom according to the mass from large to small, and the automatic separation module controls the liquid layers of the layered liquid to pass through the liquid flow module from top to bottom step by step;

[0028] S2: The self-contrast recognition module obtains the layering condition of the liquid flowing through the liquid flow module, and transmits the collected data to the central processing module in real time;

[0029] S3: When the central processing module processes the obtained data and identifies the layering boundary of the target liquid layer and the adjacent liquid layer, the central processing module sends an instruction to the automatic separation module to control the liquid flow of the liquid flow module to be interrupted;

[0030] If the target liquid layer is located at the upper end or the lower end, the separation of the target liquid layer and other liquid layers is completed, and the separation is ended;

[0031] When the target liquid layer is located in the middle liquid layer, the next step is performed;

[0032] S4: complete the separation of the mixed liquid layer containing the target liquid layer and other liquid layers, re-layer the mixed liquid layer containing the target liquid layer, and place the layered liquid in the liquid flow module again for transportation. The automatic separation module controls the liquid layers of the layered liquid to pass through the liquid flow module from top to bottom step by step, and repeats step 2.

[0033] It should be noted that after centrifugal separation of the original liquid, the distribution of the liquid layers is different according to the different densities, and the layering from bottom to top after centrifugation is different:

[0034] For example, after centrifugal layering of blood, the layering sequence from bottom to top is red blood cell liquid, platelet concentrate liquid, serum liquid, and the distribution of regenerative factors is in the platelet concentrate liquid. If the platelet concentrate liquid layer containing regenerative factors needs to be separated out, two-step separation operation is required: the first step needs to separate out the serum liquid and the platelet concentrate liquid layer in the upper layer, remove the red blood cell liquid layer, or separate out the serum liquid, separate out the platelet concentrate liquid and the red blood cell liquid layer, and obtain the mixed liquid of the platelet concentrate liquid and another liquid layer. After centrifugation again, clear layered liquid layers are obtained; the second step separates the centrifuged and layered liquid layers, which can accurately separate and achieve good liquid layer separation effect, and can obtain platelet concentrate liquid containing stable regenerative factors.

[0035] For example, after centrifugal layering of a cell suspension, it is divided into two layers, which are cell sediment layer and supernatant layer from bottom to top. When separating the liquid layers, only one step operation is required to separate the cell sediment layer and the supernatant layer, achieving the separation purpose.

[0036] For example, after centrifugal layering of a cell suspension, it is divided into two layers, which are cell sediment layer and supernatant layer from bottom to top. When separating the liquid layers, only one step operation is required to separate the cell sediment layer and the supernatant layer, achieving the separation purpose.

[0037] The separation method of the centrifugal liquid layer separation system of the present application can be performed according to different layered liquids, and the target liquid layer is located at different positions to achieve the separation purpose.

[0038] It should be noted that the method of centrifuging the liquid can be realized by using the existing technology, and the purpose is to separate the liquid layers with different mass or density in the liquid, and the specific centrifugal method does not affect the realization of the purpose of the present application.

[0039] Compared with the prior art, the present application has the following advantages:

[0040] (1) The present application identifies the stratification of the liquid flowing through the self-comparison recognition module, and transmits the recognition information to the central processing module in real time. When different liquid layer changes are identified, the central processing module sends instructions to the automatic separation module, so that the automatic separation module controls the on-off state of the liquid flow, and the purpose of automatically and intelligently separating different liquid layers is achieved.

[0041] (2) The present application applies the centrifugal liquid layer separation system, designs an automatic device to replace manual operation for subsequent separation operation of the stratified liquid, reduces the error caused by manual identification, standardizes the separation operation, improves the quality stability of the separated liquid layer, and reduces the quality fluctuation of the same batch of products. BRIEF DESCRIPTION OF DRAWINGS

[0042] Figure 1 It is a connection diagram of the centrifugal liquid layer separation system in the present application;

[0043] Figure 2 It is a structure diagram of the centrifugal liquid layer separation system in the present application;

[0044] Figure 3 It is Figure 2 the local enlarged view of A in the present application;

[0045] Figure 4 It is a flow chart of the separation method of the centrifugal liquid layer separation system in the present application.

[0046] The illustration marks are explained as follows:

[0047] 1-liquid flow module, 2-self-comparison recognition module, 21-light source module, 22-light recognition module, 3-automatic separation module, 31-guide, 32-moving seat, 4-central processing module, 41-signal processing module, 42-control module, 5-main body, 51-display, 52-control panel, 6-recognition chamber, 61-flow channel, 611-recognition tube, 62-separation tube, 621-liquid carrier tube, 622-piston, 624-push rod. DETAILED DESCRIPTION

[0048] The present application will be further described below in combination with specific embodiments. The drawings are only used for exemplary description, and the representation is only a schematic diagram, not a physical diagram, and cannot be understood as a limitation of the present patent; in order to better illustrate the embodiments of the present application, some components in the drawings may be omitted, enlarged or reduced, and do not represent the size of the actual product; for those skilled in the art, it is understandable that some known structures and their descriptions in the drawings may be omitted.

[0049] The same or similar reference numerals in the drawings of the embodiments of the present application correspond to the same or similar components; in the description of the present application, it is understood that if the orientations or positional relationships indicated by the terms "upper", "lower", "left", "right", etc. are based on the orientations or positional relationships shown in the drawings, they are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, therefore the terms describing the positional relationships in the drawings are only used for exemplary illustration and cannot be understood as a limitation on the present patent, for those skilled in the art, the specific meanings of the above terms can be understood according to the specific circumstances.

[0050] Embodiment 1

[0051] As shown in Figures 1 to 2 , a centrifugal liquid layer separation system, comprising a liquid flow module 1, a self-contrast recognition module 2 for obtaining the layering condition of the liquid flowing through the liquid flow module 1, an automatic separation module 3 connected to the liquid flow module 1 and controlling the on-off state of the liquid flow, and a central processing module 4 connected to the self-contrast recognition module 2 and the automatic separation module 3 respectively.

[0052] In this way, the layered liquid is transported from the liquid flow module 1, the self-contrast recognition module 2 identifies the layering condition of the flowing liquid, and transmits the identification information to the central processing module 4 in real time, when different liquid layer changes are identified, the central processing module 4 sends instructions to the automatic separation module 3, so that the automatic separation module 3 controls the on-off state of the liquid flow, achieving the purpose of automatically and intelligently separating different liquid layers.

[0053] As shown in Figure 2 , the liquid flow module 1 comprises a separation site for loading the layered liquid after centrifugation and a liquid receiving site for loading the liquid layer after separation, and a detachable separation tube 62 is arranged on the separation site and the liquid receiving site respectively, and the separation tubes 62 on the separation site and the liquid receiving site are connected to each other through an identification tube 611.

[0054] In this embodiment, the self-contrast recognition module 2 comprises a light source module 21 for irradiating the flowing liquid, and a light recognition module 22 for recognizing the light absorption value of the flowing liquid, and the light recognition module 22 is connected to the central processing module 4.

[0055] In this embodiment, the light source module 21 is a constant visible light.

[0056] Thus, the constant visible light irradiation emitted by the light source module 21 passes through the liquid, the visible light source does not cause damage to the platelets, the light recognition module 22 of the receiving part recognizes itself and is compared with the serum and red blood cells in the same tube, does not contact the liquid, and different liquid layers have different light absorption values and frequencies. In this way, the light absorption value of the passing liquid is recognized, and a real-time receiving and feedback recognition module is formed.

[0057] In this embodiment, the central processing module 4 includes a signal processing module 41 and a control module 42 connected to the signal processing module 41, the signal processing module 41 is connected to the light recognition module 22, and the control module 42 is connected to the automatic separation module 3.

[0058] In this embodiment, the signal processing module 41 removes the individual differences of the passing liquid by coupling algorithm processing, reduces the recognition error, and recognizes the stratification change of the liquid.

[0059] In this way, the recognition error caused by the different light absorption values of the individual differences of the liquid is removed by coupling algorithm processing of the light absorption value, and the purpose of accurately separating the liquid layer where the regenerative factor is located is achieved.

[0060] Embodiment 2

[0061] As shown in Figures 1 to 3 A centrifugal liquid layer separation system includes a liquid flow module 1, a self-comparison recognition module 2 for obtaining the stratification of the passing liquid in the liquid flow module 1, an automatic separation module 3 connected to the liquid flow module 1 and controlling the on-off state of the liquid flow, and a central processing module 4 connected to the self-comparison recognition module 2 and the automatic separation module 3, respectively.

[0062] In this way, the stratified liquid is transported from the liquid flow module 1, the self-comparison recognition module 2 recognizes the stratification of the passing liquid, and the recognition information is transmitted to the central processing module 4 in real time. When the different liquid layer changes are recognized, the central processing module 4 sends instructions to the automatic separation module 3, so that the automatic separation module 3 controls the on-off state of the liquid flow, and the purpose of automatically and intelligently separating different liquid layers is achieved.

[0063] In this embodiment, the liquid flow module 1 includes a separation site for loading the stratified liquid after centrifugation and a liquid receiving site for loading the liquid layer after separation. The separation site and the liquid receiving site are respectively provided with a detachable separation tube 62. The separation tubes 62 on the separation site and the liquid receiving site are connected to each other through an identification tube 611.

[0064] In this embodiment, the self-comparison recognition module 2 includes a light source module 21 for irradiating the passing liquid and a light recognition module 22 for recognizing the light absorption value of the passing liquid. The light recognition module 22 is connected to the central processing module 4.

[0065] In this embodiment, the light source module 21 is a constant visible light.

[0066] In this way, the constant light emitted by the light source module 21 irradiates the flowing liquid, and the visible light source does not cause damage to the platelets. The light recognition module 22 of the receiving part recognizes itself by contrast, and performs contrast with the serum and red blood cells in the same tube. The liquid does not come into contact with the liquid, and different liquid layers have different light absorption values and frequencies. In this way, the light absorption value of the flowing liquid is recognized, and a real-time receiving and feedback recognition module is formed.

[0067] In this embodiment, the central processing module 4 includes a signal processing module 41 and a control module 42 connected to the signal processing module 41. The signal processing module 41 is connected to the light recognition module 22, and the control module 42 is connected to the automatic separation module 3.

[0068] In this embodiment, the signal processing module 41 removes the individual differences of the flowing liquid by coupling algorithm processing, reduces the recognition error, and recognizes the stratified changes of the liquid.

[0069] In this way, the recognition error caused by the different light absorption values of the individual differences of the liquid is removed by coupling algorithm processing of the light absorption value, so as to accurately separate the liquid layer where the regenerative factor is located.

[0070] As shown in Figure 2 and Figure 3 The centrifugal liquid layer separation system also includes an identification chamber 6 with an opening and closing door. A flow channel 61 is formed in the identification chamber 6. The two ends of the flow channel 61 are respectively connected to the separation position and the liquid receiving position. An identification tube 611 is located in the flow channel 61. The light source module 21 and the light recognition module 22 are respectively arranged on the two sides of the flow channel 61.

[0071] In this embodiment, the flow channel 61 is an arc-shaped flow channel.

[0072] The automatic separation module 3 includes a guide 31 coaxially arranged with the separation tube 62 on the separation position, a moving seat 32 connected to the guide 31, and a driving module for reciprocating the moving seat 32 on the guide 31. The driving module is connected to the control module 42.

[0073] The identification chamber 6 is provided with an opening and closing type. On the one hand, it is convenient for the connection and disassembly of the separation tube 62 on the separation position and the liquid receiving position. On the other hand, the identification chamber 6 can be closed during work, so that the identification chamber 6 is in airtight and darkroom state, so as to ensure that the self-contrast recognition module 2 using light recognition is not affected by external light, and improve the recognition accuracy. The flow channel 61 and the built-in identification hose 611 can control the flux of the liquid discharged from the separation tube 62 on the separation position, prolong the flow path, and ensure the recognizable area of the recognition module 2, so as to improve the recognition accuracy.

[0074] AsFigure 2 As shown, the separation tube 62 comprises a liquid carrying tube 621 with two open ends, a piston 622 movably arranged in one open end of the liquid carrying tube 621, and a push rod 624 detachably coupled with the piston 622, and the push rod 624 is detachably connected with the moving seat 32.

[0075] The detachable design of the separation tube 62 can realize fast liquid suction when the push rod 624 is installed during liquid loading, and can realize separate centrifugal operation of the liquid in the liquid carrying tube 621 to achieve layering when the push rod 624 is detached. When the separation operation of the layered liquid is performed, the liquid carrying tube 621 with the detached push rod 624 is more flexible and convenient to install and detach, and the connection between the push rod 624 and the moving seat 32 is also more flexible and convenient, which is convenient for the separation operation of different volumes of liquid in the liquid carrying tube 621.

[0076] As shown in Figure 3 The center processing module 4 further comprises a display 51 and a control panel 52, and the display 51 and the control panel 52 are respectively connected with the control module 42.

[0077] The start, stop, control factor concentration, and separation threshold value of the separation process automatic separation module 3 can be controlled through the control panel 52, and the state, separation steps, and separation parameters of the liquid layer during the liquid separation process can be directly and intuitively read through the display 51.

[0078] In this embodiment, the liquid flow module 1, the light source module 21 and the light recognition module 22, the liquid flow module 1, the center processing module 4, the recognition chamber 6, the flow channel 61, the separation tube 62, the display 51 and the control panel 52, the guide 31, the moving seat 32, and the driving module are all packaged on the main body 5, so that the centrifugal liquid layer separation system in this embodiment becomes an independent device as a whole.

[0079] In this way, the subsequent separation operation of the layered liquid is replaced by the designed automatic device, the error caused by manual recognition is reduced, the operation of standardizing the separation and regeneration of the factor concentrate is improved, the quality stability of the separated liquid layer is improved, and the quality fluctuation of the products in the same batch is reduced.

[0080] Embodiment 3

[0081] As shown in Figure 1 and Figure 4 A separation method of a centrifugal liquid layer separation system:

[0082] In this embodiment, the layered liquid is layered blood, and the target liquid layer is the platelet concentrate layer. After centrifugal separation of the original blood, the liquid layers are distributed according to the different densities, and the order of the layers from the bottom to the top is the red blood cell liquid, the platelet concentrate, the serum liquid. The regeneration factor is distributed in the platelet concentrate. The platelet concentrate in the layer where the regeneration factor is located is separated out, and the specific steps include the following steps.

[0083] S1: The layered liquid is placed in the liquid flow module 1 for transportation. The liquid layers of the layered liquid are sequentially layered from the bottom to the top according to the different masses, and the automatic separation module 3 controls the liquid layers of the layered liquid to pass through the liquid flow module 1 from the top to the bottom step by step.

[0084] S2: The self-control recognition module 2 acquires the layering condition of the liquid flowing through the liquid flow module 1, and transmits the collected data to the central processing module 4 in real time.

[0085] S3: When the central processing module 4 processes the data and identifies that the platelet concentrate layer where the regeneration factor is located has passed through the liquid flow module 1, the central processing module 4 sends an instruction to the automatic separation module to control the liquid flow module 1 to interrupt the flow, and completes the separation of the mixed liquid of the platelet concentrate and the serum liquid from the red blood cell liquid.

[0086] S4: The mixed liquid of the platelet concentrate layer and the serum liquid layer that has passed through is layered again, and the layered liquid is placed in the liquid flow module 1 for transportation again. The liquid layers of the layered liquid are distributed according to the different masses, and the order of the layers from the bottom to the top is the platelet concentrate and the serum liquid. The automatic separation module 3 controls the liquid layers of the layered liquid to pass through the liquid flow module 1 from the top to the bottom step by step, and step 2 is repeated.

[0087] S5: When the central processing module 4 processes the data and identifies that the platelet concentrate layer where the regeneration factor is located starts to pass through the liquid flow module 1, the central processing module 4 sends an instruction to the automatic separation module 3 to control the liquid flow module 1 to interrupt the liquid flow, and completes the separation of the platelet concentrate from the serum liquid.

[0088] In this embodiment, since the target liquid layer is located in the middle liquid layer, two separation operations are required: in the first step, the serum liquid and platelet concentrate liquid layers located in the upper layer are separated out, the red blood cell liquid layer is removed, and a mixed liquid of serum liquid and platelet concentrate liquid is obtained. Since the serum liquid and platelet concentrate liquid are easier to separate after separation, the clear layered liquid layers are obtained after centrifugation operation is performed again; in the second step, the platelet concentrate liquid and serum liquid that have been centrifuged and layered are separated. The upper serum liquid passes through the blood circulation module first, and when the serum liquid layer is identified by the self-contrast recognition module 2 to pass through completely and the platelet concentrate liquid starts to pass through the blood circulation module 1, the continuous circulation of the platelet concentrate liquid in the blood circulation module 1 is blocked, and the platelet concentrate liquid that has passed a distance can also be guided back by the automatic separation module 3, so that the separation can be more accurate, the liquid layer separation effect is good, and the platelet concentrate liquid in which the regenerative factor is stably concentrated can be obtained.

[0089] Embodiment 4

[0090] As shown in FIG. 1, a separation method of a centrifugal liquid layer separation system includes the following steps: Figures 1 to 4

[0091] In this embodiment, the layered liquid is layered blood, and the target liquid layer is a platelet concentrate liquid layer. After the original blood is centrifuged and separated, the liquid layers are distributed according to the density difference, and the layered order from the bottom to the top after centrifugation is red blood cell liquid, platelet concentrate liquid, serum liquid, and the regenerative factor is distributed in the platelet concentrate liquid. The platelet concentrate liquid in which the regenerative factor is located needs to be separated out, and the specific steps include the following steps:

[0092] S1: The liquid is transferred into the liquid loading pipe 621 in a safe and non-polluted manner, and the liquid loading pipe 621 containing the liquid is subjected to centrifugation treatment, so that the liquid in the liquid loading pipe 621 is layered and reaches the separation standard;

[0093] S2: The liquid loading pipe 621 containing the liquid is placed in the separation position, the empty liquid loading pipe 621 is placed in the liquid receiving position, the two liquid loading pipes 621 are connected through the identification pipe 611, the push rod 624 of the liquid loading pipe 621 in the separation position is installed on the moving seat 32, and the identification chamber 6 is closed;

[0094] S3: The driving module is started through the control panel 52, the driving module drives the moving seat 32 to move along the guide piece 31, the push rod 624 installed on the moving seat 32 gradually approaches the piston 622 inside the liquid loading pipe 621 in the separation position, the push rod 624 and the piston 622 are buckled after the push rod 624 contacts the piston 622, the piston 622 is gradually pushed by the push rod 624 to extrude the layered liquid in the liquid loading pipe 622, and the liquid is gradually guided to the liquid loading pipe 621 in the liquid receiving position through the identification pipe 611;

[0095] ​S3: The liquid in the identification tube 611 is irradiated by the light source module 21, and the light recognition module 22 obtains the light absorption value of the flowing liquid. The signal processing module 41 continuously receives the data collected by the light recognition module 22 and continuously records, and according to the change amplitude and frequency of the absorption value, removes the individual difference of the liquid, identifies the stratification change of the liquid and continuously transmits the processing information to the control module 42;

[0096] S5: When the light recognition module 22 identifies that the liquid layer containing the regenerative factor has entered the liquid receiving pipe 622, the control module 42 sends a command to stop the movement of the moving seat 32.

[0097] S6: The liquid receiving pipe 622 is removed, and the liquid receiving pipe 622 is centrifuged again to separate the liquid layer in the liquid receiving pipe 622 to the separation standard, and the liquid receiving pipe 622 meeting the separation standard is installed in the separation position. Another empty liquid receiving pipe 622 is installed in the liquid receiving position, and steps 2 to 4 are repeated.

[0098] S7: When the light recognition module 22 identifies that the liquid layer containing the regenerative factor enters the identification tube 611, the control module 42 sends a command to move the moving seat 32 in the opposite direction, so that the liquid layer containing the regenerative factor returns to the liquid receiving pipe 622 in the separation position, achieving the purpose of separating the liquid layer containing the regenerative factor.

[0099] In this embodiment, since the target liquid layer is located in the middle liquid layer, two separation operations are required: in the first step, the upper serum liquid and platelet concentrate liquid layer are separated into the liquid receiving pipe 622 in the separation position to obtain the mixed liquid of serum and platelet concentrate. Since the serum and platelet concentrate are easier to separate after separation, clear stratified liquid layer can be obtained after centrifugation. In the second step, the separation pipe 62 is placed in the separation position for separation operation, and the upper serum liquid is introduced into the liquid receiving position from the separation position, so that the platelet concentrate containing the regenerative factor with good separation effect and stable concentration multiple can be obtained.

[0100] Embodiment 5

[0101] As shown in Figure 1 and Figure 4 , a separation method of a centrifugal liquid layer separation system:

[0102] The embodiment is similar to embodiment 3, and the difference is that in the embodiment, the first step is to remove the upper serum liquid first to obtain a mixed liquid of red blood cell liquid and platelet concentrate liquid, and then the centrifugal operation is performed again to obtain a clear layered liquid layer; the second step is to separate the platelet concentrate liquid and the red blood cell liquid which have been centrifugally layered, the platelet concentrate liquid passes through the blood flow module first, and the self-contrast recognition module 2 recognizes that the platelet concentrate liquid layer has passed completely, that is, the blood flow in the blood flow module 1 is blocked, so that the separation can be more accurate, the liquid layer separation effect is good, and the platelet concentrate liquid in which the regenerative factor is stable in concentration can be obtained.

[0103] The embodiment specifically includes the following steps:

[0104] S1: The layered liquid is placed in the liquid flow module 1 for transportation, and the liquid layers of the layered liquid are sequentially layered from top to bottom according to the quality from large to small, and the automatic separation module 3 controls the liquid layers of the layered liquid to pass through the liquid flow module 1 from top to bottom step by step;

[0105] S2: The self-contrast recognition module 2 acquires the layered condition of the liquid flowing through the liquid flow module 1, and transmits the collected data to the central processing module 4 in real time;

[0106] S3: When the central processing module 4 processes the data and recognizes that the platelet concentrate liquid layer in which the regenerative factor is located has passed through the liquid flow module 1, the central processing module 4 sends an instruction to the automatic separation module to interrupt the flow of the liquid flow module 1, and completes the separation of the mixed liquid of serum liquid and platelet concentrate liquid and red blood cell liquid;

[0107] S4: The mixed liquid of platelet concentrate liquid and red blood cell liquid which does not pass through is layered again, and the layered liquid is placed in the liquid flow module 1 for transportation again, the liquid layers of the layered liquid are distributed according to the quality, and the layered sequence from bottom to top is red blood cell liquid, platelet concentrate liquid, the automatic separation module 3 controls the liquid layers of the layered liquid to pass through the liquid flow module 1 from top to bottom step by step, and step 2 is repeated;

[0108] S5: When the central processing module 4 processes the data and recognizes that the platelet concentrate liquid layer in which the regenerative factor is located has passed through the liquid flow module 1, the central processing module 4 sends an instruction to the automatic separation module 3 to interrupt the flow of the liquid flow module 1, and completes the separation of the red blood cell liquid and the platelet concentrate liquid.

[0109] Embodiment 6

[0110] As shown in Figure 1 and Figure 4 Figure 1 , a separation method of a centrifugal liquid layer separation system,

[0111] The embodiment is similar to embodiment 3, and the difference is that, in the embodiment, the layered liquid is an exosome-containing solution, the exosome-containing solution is divided into two layers after centrifugation, and the exosome precipitate layer and the supernatant layer are from bottom to top, respectively, and the target liquid layer of the embodiment is the exosome precipitate layer.

[0112] The embodiment specifically comprises the following steps:

[0113] S1: The layered liquid is placed in the liquid flow module 1 for transportation, and the liquid layers of the layered liquid are sequentially layered from bottom to top according to the mass from large to small, and the automatic separation module 3 controls the liquid layers of the layered liquid to pass through the liquid flow module 1 from top to bottom step by step;

[0114] S2: The self-control recognition module 2 acquires the layering condition of the liquid flowing through the liquid flow module 1, and transmits the collected data to the central processing module 4 in real time;

[0115] S3: When the central processing module 4 processes the data and identifies that the supernatant layer has passed through the liquid flow module 1, the central processing module 4 sends an instruction to the automatic separation module to control the flow interruption of the liquid flow module 1, and completes the separation of the supernatant layer and the exosome precipitate layer.

[0116] Obviously, the above embodiments of the present application are only examples for clearly illustrating the present application, and are not intended to limit the embodiments of the present application. For those skilled in the art, on the basis of the above description, other different forms of changes or variations can also be made. Here, it is not necessary and impossible to exhaust all the embodiments. Any modification, equivalent replacement and improvement made within the spirit and principle of the present application should be included in the protection scope of the claims of the present application.

Claims

1. A centrifugal liquid layer separation system, characterized by, The application relates to a centrifugal liquid layer separation system, which comprises a liquid flow module (1), a self-contrast recognition module (2) for acquiring the stratification of liquid flowing through the liquid flow module (1), an automatic separation module (3) connected with the liquid flow module (1) and used for controlling the on-off state of liquid flow, and a central processing module (4) connected with the self-contrast recognition module (2) and the automatic separation module (3) respectively; the self-contrast recognition module (2) comprises a light source module (21) used for irradiating the flowing liquid and a light recognition module (22) used for recognizing the light absorption value of the flowing liquid, the light recognition module (22) is connected with the central processing module (4); the central processing module (4) comprises a signal processing module (41) and a control module (42) connected with the signal processing module (41), the signal processing module (41) is connected with the light recognition module (22), and the control module (42) is connected with the automatic separation module (3); the signal processing module (41) removes the individual difference of the flowing liquid through a coupling algorithm, reduces the recognition error, and recognizes the stratification change of the liquid.

2. The centrifugal liquid layer separation system of claim 1, wherein, The liquid flow module (1) comprises a separation position used for loading the stratified liquid after centrifugation and a liquid receiving position used for loading the liquid layer after separation, and detachable separation tubes (62) are arranged on the separation position and the liquid receiving position respectively; the separation tubes (62) on the separation position and the liquid receiving position are communicated with each other through a recognition tube (611).

3. The centrifugal liquid layer separation system of claim 1, wherein, The light source module (21) is one of a constant light source or a combined light source.

4. The centrifugal liquid layer separation system of claim 2, wherein, The centrifugal liquid layer separation system further comprises a recognition chamber (6) provided with an opening and closing door, a flow channel (61) is arranged in the recognition chamber (6), the two ends of the flow channel (61) are communicated with the separation position and the liquid receiving position respectively, the recognition tube (611) is arranged in the flow channel (61), and the light source module (21) and the light recognition module (22) are arranged on the two sides of the flow channel (61) respectively; the automatic separation module (3) comprises a guide part (31) coaxially arranged on the separation tube (62) on the separation position, a moving base (32) connected with the guide part (31), and a driving module used for reciprocating the moving base (32) on the guide part (31), and the driving module is connected with the control module (42).

5. The centrifugal liquid layer separation system of claim 4, wherein, The separation tube (62) comprises a liquid loading tube (621) with two open ends, a piston (622) movably arranged in one end of the liquid loading tube (621), and a push rod (624) detachably buckled with the piston (622), the push rod (624) is detachably connected with the moving base (32), and the two ends of the recognition tube (611) are communicated with the liquid loading tubes (621) of the separation position and the liquid receiving position respectively.

6. The centrifugal fluid layer separation system of claim 1, wherein, The central processing module (4) further comprises a display (51) and a control panel (52), and the display (51) and the control panel (52) are connected with the control module (42) respectively.

7. A separation method of a centrifugal liquid layer separation system, characterized by, The application further discloses a centrifugal liquid layer separation method. The application relates to a centrifugal liquid layer separation system, which comprises a liquid flow module (1), a self-contrast recognition module (2) for acquiring the stratification of liquid flowing through the liquid flow module (1), an automatic separation module (3) connected with the liquid flow module (1) and used for controlling the on-off state of liquid flow, and a central processing module (4) connected with the self-contrast recognition module (2) and the automatic separation module (3) respectively; the self-contrast recognition module (2) comprises a light source module (21) used for irradiating the flowing liquid and a light recognition module (22) used for recognizing the light absorption value of the flowing liquid, the light recognition module (22) is connected with the central processing module (4); the central processing module (4) comprises a signal processing module (41) and a control module (42) connected with the signal processing module (41), the signal processing module (41) is connected with the light recognition module (22), and the control module (42) is connected with the automatic separation module (3); the signal processing module (41) removes the individual difference of the flowing liquid through a coupling algorithm, reduces the recognition error, and recognizes the stratification change of the liquid. The application further discloses a centrifugal liquid layer separation method. S1: the layered liquid is placed in the liquid flow module (1) for transportation, the liquid layers of the layered liquid are sequentially layered from top to bottom according to the mass from large to small, and the automatic separation module (3) controls the liquid layers of the layered liquid to pass through the liquid flow module (1) from top to bottom step by step; S2: the self-contrast recognition module (2) acquires the layering condition of the liquid flowing through the liquid flow module (1), and transmits the collected data to the central processing module (4) in real time; S3: when the central processing module (4) processes the acquired data and identifies the layering boundary of the target liquid layer and the adjacent liquid layer, the central processing module (4) sends an instruction to the automatic separation module (3) to control the liquid flow in the liquid flow module (1) to be interrupted; if the target liquid layer is located at the upper end or the lower end liquid layer, the separation of the target liquid layer and other liquid layers is completed, and the separation is ended; if the target liquid layer is located at the middle liquid layer, the next step is executed; S4: the separation of the mixed liquid layer containing the target liquid layer and other liquid layers is completed, the mixed liquid layer containing the target liquid layer is layered again, the layered liquid is placed in the liquid flow module (1) for transportation again, the automatic separation module (3) controls the liquid layers of the layered liquid to pass through the liquid flow module (1) from top to bottom step by step, and step 2 is repeated.

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