Use of a formulation containing plant extracts for plant growth regulating activity

Abstract

The application provides the field of agriculture, and in particular relates to the application of a formula combination containing plant extracts in plant growth regulation activity. The active components of the formula combination are active ingredient A and active ingredient B. The active ingredient A is persimmon tannin, and the active ingredient B is chitooligosaccharide. Compared with single agents, the plant growth regulation formula combination of the application has more remarkable effects, can enhance the stress resistance of crops, promote crop growth, increase crop yield, improve the fruit quality of crops, and can be used as a detasseling agent.

Description

Technical Field

[0001] This invention relates to the agricultural field, and more specifically to the application of a formulation containing plant extracts in regulating plant growth activity. Background Technology

[0002] Plant growth regulators are a class of hormones widely used in agricultural production to regulate plant growth and development. They effectively regulate plant growth and development, enhance stress resistance, increase yield, and improve quality. They are characterized by low dosage, rapid effect, low residue, and strong targeting. Based on their effects on growth, they can be divided into plant growth promoters, plant growth inhibitors, and plant growth retardants, with the choice depending on the plant variety and application purpose. Common growth promoters include indolepropionic acid, naphthaleneacetic acid, and 6-benzyladenine; common growth inhibitors include triiodobenzoic acid and chlormequat chloride; and common growth retardants include uniconazole, paclobutrazol, and chlormequat chloride. Studies have found that the combined or sequential use of two or more plant growth regulators often produces a greater effect than single application, better leveraging their regulatory functions. However, the combined use of two or more plant growth regulators can also inhibit crop growth, leading to lower yields or yield increases that are less than those achieved by single application. Therefore, the rational use of plant growth regulators is crucial.

[0003] Preparing high-yield hybrid seeds using growth regulators is an important method for increasing grain yield. A key issue in growth regulator breeding is finding excellent regulators that achieve high emasculation rates without affecting female organs. Emasculation agents are an important agricultural application of growth regulators. Currently, there are few types of emasculation agents, and most are chemically synthesized products, such as maleic hydrazine. The market lacks green chemical emasculation agents, making this a major direction of current research.

[0004] In recent years, plant growth regulators have received increasing attention. They can not only reduce agricultural costs, but also improve crop growth and yield, enhance fruit quality and fertilizer utilization efficiency, protect the soil environment, and reduce the occurrence of diseases. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide an application of a formulation containing plant extracts in regulating plant growth activity.

[0006] Technical approach 1: Persimmon tannin extracted from green persimmons is used as an active ingredient and added to chitosan oligosaccharide in a certain proportion to form a new plant growth regulator that can promote plant growth and development, increase plant yield, improve crop resistance, and improve crop quality.

[0007] Technical approach two: Persimmon tannin extracted from green persimmons is used as an active ingredient and added with chitosan oligosaccharide in a certain proportion to form a new green chemical plant emasculator, which can be used for high-yield hybrid seed breeding.

[0008] The technical solution adopted in this invention is as follows:

[0009] A formulation for regulating plant growth activity, characterized in that the formulation comprises active ingredient A and active ingredient B, wherein active ingredient A is persimmon tannin, active ingredient B is chitosan oligosaccharide, and the weight ratio of active ingredient A to active ingredient B is 1-20:20-200.

[0010] In the above technical solution, the formula is used to promote plant growth, and the weight ratio of active ingredient A to active ingredient B is 1-2.5:75-100.

[0011] Furthermore, the concentration range of active ingredient A is 0.1-0.25 mg / L, and the concentration range of active ingredient B is 7.5-10 mg / L.

[0012] In the above technical solution, the formula is used as a plant emasculator, and the weight ratio of active ingredient A to active ingredient B is 1-3:1-10.

[0013] Furthermore, the concentration range of active ingredient A is 200mg-300mg / L, and the concentration range of active ingredient B is 500mg-1000mg / L.

[0014] Furthermore, the concentration range of active ingredient A is 200 mg / L, and the concentration range of active ingredient B is 500 mg / L.

[0015] In the above technical solution, the active ingredient A is derived from persimmon tannin extracted from green persimmons.

[0016] Technical approach three: Apply the plant growth regulator composition prepared above to chili peppers and other crops such as peanuts, wheat, rice and potatoes.

[0017] Based on the above technical concept, the technical solution adopted by this invention is as follows:

[0018] A formulation of a plant growth-regulating activity for use in agriculture to promote or regulate crop growth or in breeding demasters.

[0019] In the above technical solution, the composition is applied to wheat, rice, peanuts, potatoes, or chili peppers.

[0020] The beneficial effects of this invention are:

[0021] 1. The active ingredients of the regulator of this invention are persimmon tannin and chitosan oligosaccharide. When compounded in a weight ratio of 1-20:20-200, it has a significant synergistic effect in regulating or promoting plant growth. The effect is best when the concentration of chitosan oligosaccharide is 7.5 mg / L and the concentration of persimmon tannin is 0.25 mg / L. It can improve crop stress resistance and crop yield, improve crop quality, and improve economic and social benefits.

[0022] 2. The active ingredients of the regulator of this invention are persimmon tannin and chitosan oligosaccharide, which have a significant synergistic effect in inducing emasculation in plants. At the concentrations of persimmon tannin + chitosan oligosaccharide of 200 mg / L + 500 mg / L, the optimal emasculation rate and cross-pollination seed setting rate of wheat are achieved, which can greatly improve breeding efficiency and promote agricultural production to increase benefits.

[0023] 3. At the same time, the frequency and amount of use of the plant growth regulator of the present invention can be adjusted according to the crop, growth environment and climate temperature, and the production purpose can be achieved by using appropriate dosage.

[0024] 4. The plant growth regulators of the present invention are all natural products, which meet the needs of green and environmentally friendly industries. They can be rapidly and naturally degraded in plants or soil, and are not likely to leave residues in plants or soil.

[0025] 5. The plant growth regulator of the present invention can promote the increase of chlorophyll content in plants, improve photosynthetic efficiency, facilitate the accumulation of dry matter in plants, and further promote the increase of yield. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below.

[0027] Example 1

[0028] Active ingredients A and B were dissolved in water and compounded according to different concentrations (Table 1).

[0029] Table 1: Combination of persimmon tannins and chitosan oligosaccharides

[0030]

[0031] Application example: Indoor potted plant experiment verification

[0032] Test subject: Gala chili peppers (provided by Harbin Jinlong Agricultural Co., Ltd.)

[0033] Experimental Method: The experiment was conducted in a glass greenhouse. Peat moss and sand were mixed evenly at a 1:1 mass ratio and placed into 54cm*27cm*6cm seedling trays. Seeds were evenly sown into the trays and covered with approximately 0.5cm of soil. Watering was done from the bottom up. Seed germination and seedling cultivation were carried out under ample sunlight and at 24-28℃. Once the pepper plants had two true leaves, seedlings of uniform growth were selected and transplanted into flowerpots. Each pot of seedlings was kept under consistent growing conditions. After the seedlings had recovered, base fertilizer was applied. The base fertilizer was a conventional compound fertilizer (15-15-15) at a rate of 600kg / hm². 2 Application. The first spray was applied 15 days after transplanting, and the second spray was applied 30 days later. Pepper yield was investigated during the fruiting period. Each treatment was replicated 5 times. The results are shown in Table 2.

[0034] The reagents included experimental group reagents, control group reagents, and blank reagents; the experimental group consisted of different combinations of chitosan oligosaccharide and persimmon tannin; the control group consisted of different concentrations of chitosan oligosaccharide or persimmon tannin; and the blank reagent was water.

[0035] Table 2: Data from the potted chili pepper experiment

[0036]

[0037]

[0038]

[0039] Table 2 shows that the pot experiment results indicate that foliar spraying of chitosan oligosaccharide and persimmon tannin both promoted the increase of chili pepper yield to varying degrees, with the compound combination in this study showing a more significant effect. The data in the table show that the most significant effects were observed when the concentration of chitosan oligosaccharide was 7.5-10 mg / L and the concentration of persimmon tannin was 0.1-0.25 mg / L. The most significant yield increase was observed when the concentration of chitosan oligosaccharide was 7.5 mg / L and the concentration of persimmon tannin was 0.25 mg / L, exceeding the yield increase of any single agent or other compound treatments, demonstrating a significant synergistic effect. This indicates that this plant growth regulator can significantly promote the accumulation and translocation of dry matter in plants, thereby increasing chili pepper yield.

[0040] Example 2

[0041] Active ingredients A and B are dissolved in water and compounded according to different concentrations (Table 3).

[0042] Table 3: Combination of persimmon tannins and chitosan oligosaccharides

[0043]

[0044] Application example: Wheat emasculation activity test

[0045] Spray the wheat field with pesticides at the concentrations shown in Table 3, 3-5 days before heading (when the main ear of wheat is 1.0-1.2 cm long). Spray 0.2 m of each concentration sample. 2 The spraying volume was 100 ml. The sterility rate was determined using a bagging isolation method. Ten ears from each plot of the female parent were bagged (B); another 10 ears that had undergone emasculation treatment but were not bagged were taken, and the number of grains after threshing was W; the control plot had an equal number of untreated ears, and the number of grains after threshing was K. The relative sterility rate (RMSR) was calculated as (KB) / K × 100%, and the relative cross-pollination seed setting rate (CFR) was calculated as (WB) / K × 100%.

[0046] Table 4: Data on relative sterility rate (RMSR) of wheat

[0047]

[0048] The data above shows that the combination of persimmon tannin and chitosan oligosaccharide has a better demasking effect on wheat than when used alone. The optimal effect is achieved at a concentration of 200 mg / L + 500 mg / L for persimmon tannin and chitosan oligosaccharide. Below this concentration, the demasking rate decreases; above this concentration, the demasking rate remains the same.

[0049] Table 5: Data on CFR (Comparative Fertility Rate) of Wheat Relative Crosscrossing

[0050]

[0051]

[0052] The data above shows that the combination of persimmon tannin and chitosan oligosaccharide has a better effect on wheat cross-pollination rate compared to using them alone. The optimal effect is achieved at concentrations of 200 mg / L + 500 mg / L for persimmon tannin and chitosan oligosaccharide. At concentrations lower than these values, the cross-pollination rate is comparable, but the emasculation rate decreases; at concentrations higher than these values, the emasculation rate is comparable, but the cross-pollination rate decreases.

[0053] The above experiments show that the optimal wheat demasing rate and cross-pollination rate are achieved when the concentration of persimmon tannin + chitosan oligosaccharide is 200 mg / L + 500 mg / L. Since persimmon tannin and chitosan oligosaccharide are green chemicals, they are expected to be used as green chemical demasing agents in the agricultural field.

[0054] The above-mentioned aqueous solution can also be applied to other crops, exhibiting a significant synergistic effect in regulating or promoting plant growth, thereby increasing crop yield and improving crop quality; it can also enhance the effectiveness of chemical hybridization breeding, greatly improving breeding efficiency. This aqueous solution can improve economic and social benefits by promoting agricultural production.

[0055] The specific embodiments of the present invention described above do not constitute a limitation on the scope of protection of the present invention. Any other corresponding changes and modifications made in accordance with the technical concept of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A formulation for regulating the growth activity of chili peppers, characterized in that, The formulation consists of active ingredient A and active ingredient B, wherein active ingredient A is persimmon tannin and active ingredient B is chitosan oligosaccharide. The concentration range of active ingredient A is 0.1-0.25 mg / L and the concentration range of active ingredient B is 7.5-10 mg / L. The active ingredient A is derived from persimmon tannin extracted from green persimmons.

2. A formulation for regulating wheat growth activity, characterized in that, The formulation consists of active ingredient A and active ingredient B, wherein active ingredient A is persimmon tannin and active ingredient B is chitosan oligosaccharide. The concentration range of active ingredient A is 200-300 mg / L and the concentration range of active ingredient B is 500-1000 mg / L. The active ingredient A is derived from persimmon tannin extracted from green persimmons.

3. The formulation combination according to claim 2, characterized in that: The concentration range of active ingredient A is 200 mg / L, and the concentration range of active ingredient B is 500 mg / L.

4. The use of the formulation combination according to claim 1 in promoting or regulating chili pepper growth in the agricultural field.

5. The use of the formulation combination according to claim 2 in wheat breeding demasking agents in the agricultural field.

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