Seed germination stimulants prepared from living nodularia sp. and uses thereof

Abstract

The present application relates to the application of living Arthrospira in the preparation of plant seed germination promoters, and seed germination promoters containing living Arthrospira. The present application uses Arthrospira to prepare germination promoters, improves the germination efficiency and seedling growth potential of plant or crop seeds under high salt conditions, thereby providing a new scheme for the management and ecological restoration of high-salt land, improving the utilization efficiency of high-salt land, shortening the restoration time, and reducing the labor cost.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of applying microalgae and crop planting, more particularly, to the application of living Arthrospira in preparing seed germination promoters of plants and the prepared seed germination promoters. BACKGROUND

[0002] High-salt land is difficult for plants to survive due to high-salt stress, and the sparseness of plants leads to the reduction of organic matter in the soil, deteriorates the soil environment, causes water and soil loss, and further increases the salt content in the soil. Therefore, the ecological restoration of high-salt land first involves vegetation restoration. There are two directions for the vegetation restoration of high-salt land: planting salt-tolerant plants or using soil conditioners to improve the soil environment so that plants that are not particularly salt-tolerant can germinate and survive in saline soil.

[0003] In order to improve the vegetation restoration effect of high-salt land, people often first improve the high-salt land by physical and chemical methods, and then plant salt-tolerant plants, but these methods are costly and have a long vegetation restoration period.

[0004] Therefore, there is a need for a new technology to improve the germination and survival of plants in high-salt environments. SUMMARY

[0005] During the research process, the present team found that applying living Arthrospira products to the environment of plant seeds can promote the germination of plant seeds and the survival of seedlings. Based on the above findings, the present application provides the application of living Arthrospira in preparing seed germination promoters of plants.

[0006] In one specific embodiment, the plant is selected from Arabidopsis thaliana and sweet sorghum.

[0007] The present application also provides a plant seed germination promoter containing living Arthrospira.

[0008] In one specific embodiment, the plant seed germination promoter is obtained by expanding the culture of the Arthrospira and then collecting the algal liquid.

[0009] In one specific embodiment, it is prepared by the following method:

[0010] S1: culturing the Arthrospira to the logarithmic phase to obtain an algal liquid;

[0011] S2: concentrating the algal liquid into algal mud, which is the plant seed germination promoter.

[0012] In one specific embodiment, the Arthrospira in S1 is cultured in Zarrouk medium.

[0013] In one specific embodiment, the algal liquid in S2 is concentrated into algal mud with a water content of 80-90%.

[0014] The present application also provides a method for promoting the germination of plant seeds in a high-salt environment, comprising the step of applying the plant seed germination promoter to the germination environment of the plant seeds.

[0015] In one specific embodiment, the concentration (wet weight / volume) of the living Arthrospira is 1-3.6 g / L.

[0016] The present application enables the promotion of the germination and seedling survival of plants or crops that are not salt-tolerant in a high-salt environment by using Arthrospira to prepare a germination promoter, thereby providing a new solution for the management and ecological restoration of high-salt land, improving the work efficiency of the management and ecological restoration of high-salt land, shortening the restoration time, and reducing labor costs. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 Photos of Arabidopsis seeds in the non-stress group and the high-salt stress group after 7 days of culture without algae or with the addition of 0.06 g / L (wet weight / volume) of fishweed Ana and Arthrospira AP.

[0018] Figure 2 Statistical curves of the germination rate of Arabidopsis seeds in the non-stress group and the high-salt stress group with the addition of 0.06 g / L (wet weight / volume) of fishweed Ana and Arthrospira AP over time and a statistical graph of the leaf greening rate after 7 days of culture.

[0019] Figure 3 Statistical graphs of the germination rate (A), germination index (B), and seedling vigor index (C) and photos of seedlings (D) of sweet sorghum seeds in the non-stress group and the high-salt stress group after incubation in an incubation solution without algae or with the addition of different concentrations of Arthrospira AP. DETAILED DESCRIPTION

[0020] The principles and features of the present application are described below in conjunction with examples, which are used only to explain the present application and are not intended to limit the scope of the present application.

[0021] 1. Cultivation and collection of living Arthrospira

[0022] Arthrospira platensis (AP) was inoculated into Zarrouk culture medium at a concentration of 1.2 g / L (wet weight / volume), and the culture was performed at a light intensity of 100 μmol m -2 s -1 30℃, and air was supplied to the culture until the logarithmic growth phase, the algal liquid was taken to a centrifuge tube and centrifuged,

[0023] 4000rpm centrifugation for 10 min, discard the supernatant, add sterile water after centrifugal washing, discard the supernatant, collect the algal cells for use.

[0024] 2. Arthrospira platensis improves the effective germination rate of Arabidopsis thaliana in high salt environment

[0025] The culture dishes were divided into CK group and high salt stress group according to the addition of 1 / 2MS medium or 1 / 2MS medium + 200Mm NaCl. In the non-stress group and high salt stress group, according to whether to add algae or not, the concentration (wet weight / volume) of 0.06g / L Lyngbya Ana and Arthrospira AP was added, which was divided into 1 / 2MS group, 1 / 2MS+Ana group and 1 / 2MS+AP group.

[0026] In each culture dish of the above experimental groups, 36 Arabidopsis thaliana seeds were scattered, the seed germination was observed daily, and the leaf greening rate was counted on the 7th day. The results are shown in Figure 1 and 2 In the CK group, the seeds of Arabidopsis thaliana under each condition all germinated on the third day, and the final leaf greening rate reached 100%. In the high salt stress group, the germination rate of Arabidopsis thaliana seeds under each condition was reduced, and the difference was not obvious; however, in terms of leaf greening rate, the leaf greening rate of Arabidopsis thaliana seeds after germination in the 1 / 2MS group was extremely low, less than 5%, the leaf greening rate of Arabidopsis thaliana seeds after germination in the 1 / 2MS+Ana group was significantly improved, but still less than 10%, the leaf greening rate of Arabidopsis thaliana seeds after germination in the 1 / 2MS+AP group reached about 25%, which was much higher than the group without adding microalgae and the group adding Lyngbya Ana.

[0027] In order to further observe the effect of Arthrospira AP on the germination of Arabidopsis thaliana seedlings, after 7 days of culture, the green seedlings with similar growth were transferred to the corresponding culture dishes for continuous culture, i.e. the seedlings in the 1 / 2MS group were transferred to the 1 / 2MS medium, the seedlings in the 1 / 2MS+Ana group were transferred to the 1 / 2MS medium with 0.06g / L Lyngbya Ana, and the seedlings in the 1 / 2MS+Ap group were transferred to the 1 / 2MS medium with 1.2mg / L Arthrospira Ap, and the sodium chloride concentration in the medium after transfer was the same as that in the medium before transfer. The results are shown in Figure 2 C, in the CK group, the leaf greening rate was still 100%. In the salt stress group, almost all the seedlings in the 1 / 2MS group turned white and could not survive; about 30% of the seedlings in the 1 / 2MS+Ana group continued to survive, and the rest turned white; about 50% of the seedlings in the 1 / 2MS+AP group continued to survive.

[0028] The above experiments show that adding live Arthrospira to the germination environment of Arabidopsis seeds can significantly improve the survival rate of Arabidopsis seedlings under high salt stress. In further experiments, we found that live Arthrospira not only promotes the germination and survival of Arabidopsis under high salt conditions, but also promotes the germination and survival of other plants such as sweet sorghum.

[0029] 3. Live *Arthrospira* species improve the effective germination rate of sweet sorghum.

[0030] Sweet sorghum seeds were placed in each petri dish and incubated with the incubation solution. The seeds were weighed every 24 hours, and water was added as needed. A certain amount of 0.2M NaCl aqueous solution or live *Syngonium argentea* solution was also added. The number of germinating seeds was observed after each 24-hour period until germination was complete. The germination rate, germination index, and seedling vigor index were calculated, and the seedling morphology was recorded.

[0031] The results are as follows Figure 3 As shown, under both no-stress and high-salt stress conditions, the addition of *Arthrophagus argyi* at a concentration (wet weight / volume) of 1.2 g / L to the incubation solution can increase the germination index of sweet sorghum seeds. Figure 3 A and B) and seedling vigor index ( Figure 3 C), and based on representative seedlings, seedlings of *Arthrospira spp.* with a concentration of 1.2 g / L exhibited healthier and more complete morphology under high salt stress.

[0032] Furthermore, the use of inactivated Spirulina or Spirulina extract in the above experiments did not produce this promoting effect.

[0033] The above experiments demonstrate that adding live *Arthrospira* to the environment during plant seed germination can effectively promote seed germination and seedling survival, especially under high salt stress. This provides a method for improving seed germination and survival of vegetation or crops in high-salt soils.

[0034] This invention exemplifies the use of *Arthrospira platensis* in the preparation of seed germination agents for illustrative purposes. In fact, our experiments have shown that other *Arthrospira* species can also promote plant seed germination, especially effectively promoting seed germination and seedling survival under high salt stress.

[0035] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. Use of a plant seed germination accelerator in promoting survival of Arabidopsis thaliana under high salt environment, characterized in that, The survival is specifically improving the leaf greening rate after seed germination, the plant seed germination promoter comprises living arthrospira platensis, and the concentration of the arthrospira platensis is 1.2 g / L (wet weight / volume).

2. Use of a plant seed germination accelerator in promoting the germination of sweet sorghum seeds under a high salt environment, characterized in that, The plant seed germination promoter comprises living arthrospira platensis, and the concentration of the arthrospira platensis is 1.2 g / L (wet weight / volume).

3. Use according to claim 1 or 2, characterized in that, The plant seed germination promoter is prepared by the following method: S1: the arthrospira platensis is cultured to the logarithmic phase to obtain algae liquid; S2: the algae liquid is concentrated into algae mud, that is, the plant seed germination promoter.

4. Use according to claim 3, characterized in that, The arthrospira platensis in S1 is cultured in Zarrouk culture medium.

5. Use according to claim 3, characterized in that, In S2, the algae liquid is concentrated into algae mud with a water content of 80-90%.

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