Device and method for multi-field enhanced biological treatment of organic phosphorus, organic chlorine and benzene contaminated soil

Abstract

The invention discloses a device for multi-external field enhanced biological treatment of soil contaminated by organophosphorus, organochlorine and benzene series, which comprises a crushing and mixing mechanism, an electromagnetic taming mechanism, an expansion culture mechanism and an inoculation and stirring mechanism. When the device is used, soil containing organophosphorus, organochlorine and benzene series pollutants is crushed, and the contaminated soil is tamed by electromagnetics to change the microbial population structure in the soil. Then, the tamed soil is inoculated into the crushed soil, and the tamed microorganisms are used to degrade the organophosphorus, organochlorine and benzene series organic pollutants in the contaminated soil, thereby achieving harmless treatment of the organically contaminated soil.

Description

Technical Field

[0001] The invention belongs to the technical field of harmless treatment of organic polluted soil, and specifically is a device for multi-field enhanced biological treatment of soil polluted by organic phosphorus, organic chlorine and benzene series organic matter. Background Art

[0002] Organic pollutants in soil have always been a major factor that troubles the effective use of soil. Although organic phosphorus is one of the important nutrients in soil, its large presence makes it easy to precipitate from the soil under the action of rainwater runoff and enter the surrounding water bodies, causing the emergence of eutrophication of water bodies, which has a serious impact on the water quality of surrounding water bodies. Organic chlorides and benzene series make it difficult for crops in the soil to survive due to their highly toxic characteristics. Even if the crops survive, they accumulate a large amount of toxicity due to the action of the food chain, making organic chlorides, benzene series and other toxic and harmful substances easily accumulate through the food chain and eventually enter the human body, seriously endangering human life, health and safety. Therefore, how to efficiently and conveniently treat soil contaminated by substances such as organic phosphorus, organic chlorine and benzene series has become one of the major problems that need to be solved in the field of environmental safety. In the past, common chemical methods were often inadequate in treating such substances, and although the use of a large number of chemical substances can reduce the environmental safety problems caused by such pollutants to a certain extent, chemical treatment methods are often prone to secondary pollution to the environment during use. Therefore, when faced with such problems, researchers have proposed using biological methods to degrade harmful pollutants in contaminated soil. However, the biggest disadvantage of biological treatment is its slow processing speed and low degradation efficiency, which greatly increases the treatment cost of such contaminated soil.

[0003] CN202121802201.5 discloses a system for electrokinetic enhanced microbial treatment of petroleum hydrocarbon contaminated soil. Although this invention uses a water curtain to improve the circulation effect of nutrients in the contaminated soil, its distribution uniformity still needs to be improved compared with the ex situ treatment with external stirring, and it only considers the effect of electrokinetic force on the improvement of microbial treatment effect, but does not consider the important role of magnetic field in promoting the improvement of microbial treatment effect. CN201910790382.5 discloses an experimental device and method for resistive heating coupled microbial remediation of organic contaminated soil. This method considers the effect of temperature field on microbial remediation of organic contaminated soil, but does not consider that other external fields such as electric field and magnetic field also have a good promoting effect on improving the degradation efficiency of microorganisms. It is difficult to achieve the trend of microbial population in contaminated soil to develop towards the dominance of functional microorganisms by simply using temperature field, and it is difficult to produce a good degradation effect of highly toxic organic pollutants. Summary of the invention

[0004] With the continuous improvement of industrial production capacity, a large number of toxic pollutants are generated in the production process. In the pharmaceutical, textile, printing and dyeing industries, organic pollution has become a huge challenge faced by such industries. Among them, organic phosphorus, organic chlorine and benzene pollution have become representatives of industrial pollution. The improper discharge of such organic pollutants has caused serious ecological safety problems in the soil around the factory. In order to solve the ecological restoration problem of soil contaminated by organic phosphorus, organic chlorine and benzene, the present invention provides a multi-field enhanced biological treatment device for organic phosphorus, organic chlorine and benzene organic pollutants in soil. The device of the present invention is to mix the interference solution with the contaminated soil and then perform electromagnetic taming. The soil part after taming enters the expansion culture mechanism for the expansion culture of functional microorganisms. After the expansion culture, the bacterial solution containing rich functional microorganisms enters the inoculation stirring device and is fully mixed with the contaminated soil and then returned to the land. The rich microorganisms are used to degrade the organic phosphorus, organic chlorine and benzene in the contaminated soil, greatly enhancing the degradation effect of the in-situ functional microorganisms on organic pollutants such as organic phosphorus, organic chlorine and benzene, and realizing the harmless treatment of the contaminated soil.

[0005] The device of the present invention comprises a crushing and mixing mechanism, an electromagnetic taming mechanism, an expansion culture mechanism, and an inoculation and stirring mechanism; wherein the crushing and mixing mechanism comprises a shell, a feed port is arranged at the top of the shell, a crushing wheel is arranged in the shell, and a toothed turntable I is arranged at the bottom of the shell; the crushing and mixing mechanism is connected with the inoculation and stirring mechanism through a spiral feeding trough; a stirring paddle driven by a stepper I is arranged in the inoculation and stirring mechanism, and the lower end of the stirring paddle is movably arranged on a stirring shaft bracket; a plurality of sprayers are arranged at the top of the inoculation and stirring mechanism, and the sprayers are connected with the expansion culture tank through a delivery pump I; an exhaust gas discharge port is opened at the top of the inoculation and stirring mechanism, and a material outlet I is arranged at the bottom of the inoculation and stirring mechanism; the expansion culture mechanism comprises a culture medium configuration tank, an expansion culture tank, and a culture medium configuration tank A solvent inlet I and a solute inlet I are provided on the top, the bottom outlet of the culture medium preparation tank is connected to the expansion culture tank through a delivery pump II, the top of the expansion culture tank is connected to the electromagnetic acclimation box through a delivery pump III, and both the culture medium preparation tank and the expansion culture tank are provided with a spiral agitator driven by a stepper motor; the electromagnetic acclimation mechanism includes an electromagnetic acclimation box and an interfering substance preparation tank, the interfering substance preparation tank is provided with a solvent inlet II and a solute inlet II, magnetic electrode plates are provided on both sides of the electromagnetic acclimation box, the magnetic electrode plates are respectively connected to the positive and negative poles of the power supply, an inclined toothed turntable II is provided at the bottom of the electromagnetic acclimation box, a material outlet II is provided at the bottom of the electromagnetic acclimation box and is located above the toothed turntable II, and the interfering substance preparation tank is connected to the electromagnetic acclimation box through a delivery pump IV.

[0006] The top of the inoculation stirring tank is provided with a bacterial liquid mixture sprayer with a spray angle of 15° to 180° and a spray flow rate of 0.2 to 15 L / min. The sprayed liquid culture solution is fully mixed with the material after being stirred by a three-stage stirring paddle;

[0007] The magnetic electrode plate is prepared by grinding a conductive material to a particle size of less than 300 meshes, placing the ground conductive material in an ethanol solution with a mass concentration of 15% to 60% to obtain a 0.1 to 0.4 g / mL conductive material-ethanol mixture, stirring and mixing at 400 to 1500 rpm to obtain a uniform mixture, adding a PANI adhesive, wherein the concentration of the PANI adhesive in the ethanol solution is 0.1 to 0.8 g / mL, stirring at 400 to 1500 rpm for 10 to 15 minutes, adding a magnetic material ground to a particle size of less than 300 meshes, wherein the concentration of the magnetic material in the ethanol solution is 0.1 to 0.4 g / mL, continuing to stir and mix at 40 to 80° C. for 40 to 80 minutes to obtain a mixed slurry, placing the obtained mixed slurry under a double-roller machine, and repeatedly rolling the mixed slurry into a sheet-like solid with a thickness of 0.1 to 10 cm, and drying the mixed slurry.

[0008] The conductive material is one of magnetic carbon, magnetic carbon silicon, graphene, carbon nanotubes, fullerene, silver, and copper;

[0009] The magnetic material is one of electrical steel, rare earth alloy, nickel-based alloy and iron oxide material;

[0010] The magnetic electrode plate is provided with holes, the hole diameter is 0.1-20 mm, the pore density is 1-50 ppi, and the applied electric field voltage is 0.2-35 V; the tilt angle of the tilted bottom plate of the electromagnetic taming box is 5°-35°.

[0011] The electromagnetically acclimated soil enters the expansion culture tank through the delivery pump III, the bacterial liquid after the expansion culture enters the bacterial liquid mixture sprayer through the delivery pump, and the liquid culture medium auxiliary ingredients enter the expansion culture tank through the culture medium configuration tank;

[0012] The temperature of the expansion culture tank is 20-40° C., and the culture medium ingredients are composed of one or more of glucose, bean cake powder, corn powder, yeast powder, potassium dihydrogen phosphate, calcium carbonate, magnesium sulfate, and vitamins;

[0013] The method of using the above device is to allow water containing added interference substances to enter the electromagnetic acclimation box from the interference substance preparation tank through the delivery pump IV, and fully mix it with soil containing organophosphorus, organochlorine and benzene series polluted for more than one year under the action of the toothed turntable II, and obtain soil containing functional microorganisms after electromagnetic acclimation. The soil enters the expansion culture tank through the delivery pump III for expansion culture; the soil contaminated with organophosphorus, organochlorine and benzene series is added to the crushing and mixing mechanism from the feed inlet, and the soil is crushed by the crushing wheel. After the expansion culture, the material enters the inoculation stirring mechanism through the delivery pump I and is fully mixed with the soil contaminated with organophosphorus, organochlorine and benzene series, and the material is discharged through the material outlet I;

[0014] The added interfering substance is selected from iron powder, KH2PO4, carbon tetrachloride, C 14H9Cl5, hexachlorocyclohexane, benzene, toluene, ethylbenzene, and xylene, their concentrations in water are 0.05~0.75mol / L, and the pH range is 4.5~8.5.

[0015] The technical effects of the present invention are as follows:

[0016] 1. The electromagnetic taming of microorganisms not only increases the degradation rate of microorganisms, but also changes the population structure of microorganisms in the soil, making functional microorganisms that are effective in degrading organic phosphorus, organic chlorine and benzene pollutants dominate in the soil;

[0017] 2. The in-situ microbial domestication and cultivation method eliminates the need to purchase external bacterial agents, greatly reducing the production cost of the contaminated soil treatment process;

[0018] 3. Significantly improve the degradation efficiency of microorganisms on organic matter in contaminated soil by expanding the cultivation of microbial inoculation;

[0019] 4. Use interference solutions containing organophosphorus, organochlorine and benzene derivatives to coordinate electromagnetic regulation to adjust the microbial population structure in contaminated soil, so that microorganisms with high tolerance and degradation performance to organophosphorus, organochlorine and benzene derivatives dominate the population structure. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the structure of the device of the present invention;

[0021] Figure 2 The pollutant content of the contaminated soil before and after treatment in Example 2;

[0022] Figure 3 The pollutant content of the contaminated soil before and after treatment in Example 3;

[0023] Figure 4 The pollutant content of the contaminated soil before and after treatment in Example 4;

[0024] In the figure: 1-feeding port; 2-crushing and mixing mechanism; 3-crushing wheel; 4-toothed turntable I; 5-screw feeding trough; 6-stepping machine; 7-sprinkler; 8-inoculation stirring mechanism; 9-stirring paddle; 10-stirring shaft bracket; 11-waste gas outlet; 12-delivery pump I; 13-material outlet I; 14-expansion culture tank; 15-culture medium preparation tank; 16-delivery pump III; 17-delivery pump II; 18-solvent inlet I; 19-electromagnetic acclimation box; 20-magnetic electrode plate; 21-delivery pump IV; 22-interference substance preparation tank; 23-solvent inlet II; 24-solute inlet II; 25-solute inlet I; 26-toothed turntable II; 27-material outlet II; 28-power supply. DETAILED DESCRIPTION

[0025] The present invention is further described in detail below by way of examples, but the protection scope of the present invention is not limited to the contents described above. Example 1

[0026] like Figure 1 As shown, the device used in this embodiment includes a crushing and mixing mechanism 2, an electromagnetic taming mechanism, an expansion culture mechanism, and an inoculation stirring mechanism 8; the crushing and mixing mechanism comprises a shell, a feed port 1 is provided at the top of the shell, a crushing wheel 3 is installed in the shell, and a toothed turntable Ⅰ4 is provided at the bottom of the shell; the crushing and mixing mechanism is connected to the inoculation stirring mechanism 8 through a spiral feeding trough 5; a stirring paddle 9 driven by a stepper 6 is provided in the inoculation stirring mechanism 8, and the lower end of the stirring paddle 9 is movably arranged on the stirring shaft bracket 10, and a plurality of sprayers 7 are arranged at the top of the inoculation stirring mechanism 8, the spray angle is 150°, and the spray flow rate is 10L / min. The sprayer 7 is connected to the expansion culture tank 14 through a delivery pump Ⅰ12, and an exhaust gas outlet 11 is provided at the top of the inoculation stirring mechanism 8, and a material outlet Ⅰ13 is provided at the bottom of the inoculation stirring mechanism; the expansion culture mechanism includes a culture medium configuration tank 15 and an expansion culture tank 14, and the culture medium configuration tank 15 is provided with a solvent inlet Ⅰ18 and a solute inlet Port Ⅰ25, the bottom outlet of the culture medium configuration tank is connected to the expansion culture tank 14 through the delivery pump Ⅱ17, and the top of the expansion culture tank 14 is connected to the electromagnetic acclimation box 19 through the delivery pump Ⅲ16. The culture medium configuration tank 15 and the expansion culture tank 14 are both provided with a spiral stirrer driven by a stepper motor; the electromagnetic acclimation mechanism includes an electromagnetic acclimation box 19 and an interfering substance configuration tank 22. The interfering substance configuration tank 22 is provided with a solvent inlet Ⅱ23 and a solute inlet Ⅱ24 on the top. Magnetic electrode plates 20 are provided on both sides of the electromagnetic acclimation box. The aperture of the holes on the magnetic electrode plates is 1mm, and the pore density is 10ppi. The magnetic electrode plates are respectively connected to the positive and negative poles of the power supply 28. An inclined toothed turntable Ⅱ26 is provided at the bottom of the electromagnetic acclimation box, and the inclination angle of the inclined bottom plate is 20°. A material outlet Ⅱ27 is provided at the bottom of the electromagnetic acclimation box and is located above the toothed turntable Ⅱ. The interfering substance preparation tank 22 is connected to the electromagnetic acclimation box 19 through the delivery pump Ⅳ21;

[0027] The magnetic electrode plate is prepared by grinding magnetic carbon to a particle size of less than 300 meshes, placing the crushed magnetic carbon in an ethanol solution with a mass concentration of 10% to prepare a 0.1 g / mL magnetic carbon-ethanol mixture, stirring and mixing at 500 rpm, adding a PANI adhesive, the concentration of the PANI adhesive in the ethanol solution is 0.6 g / mL, stirring at 500 rpm for 15 minutes, adding electrical steel ground to less than 300 meshes, the concentration of the electrical steel in the ethanol solution is 0.1 g / mL, continuing to stir and mix at 60°C for 50 minutes to obtain a mixed slurry, placing the obtained mixed slurry under a double-roller machine, repeatedly rolling it into a sheet-like solid with a thickness of 1 cm, and drying it to obtain;

[0028] The above device was used to treat soil contaminated by wastewater from the printing and dyeing industry. The organic phosphorus content in the soil was 953 mg / L, the organic chloride content was 23.4 mg / L, and the toluene content was 13.44 mg / L. The added interference substances included iron powder 0.1 g / mL, KH2PO4 0.05 mol / L, carbon tetrachloride 0.05 mol / L, and toluene 0.05 mol / L, and the pH was 5.0. The expansion culture medium was an aqueous solution containing 3% glucose, 2% bean cake powder, 1% corn powder, 0.5% yeast powder, 0.1% potassium dihydrogen phosphate, 0.2% calcium carbonate, 0.05% magnesium sulfate, and 0.05% vitamins, and the pH was 5.0.

[0029] The water containing the added interference substance enters the electromagnetic acclimation box 19 from the interference substance preparation tank 22 through the delivery pump IV 21, and is fully mixed with the soil polluted by the wastewater of the printing and dyeing industry for 2 years under the action of the toothed turntable II 26. The electric field voltage of 5V is applied to acclimate the soil microorganisms under the electromagnetic action. After acclimation for 48 hours, the soil capable of treating organic pollutant microorganisms is obtained. The soil enters the expansion culture tank 14 through the delivery pump III 16 for expansion culture at 30°C; at the same time, the soil polluted by the wastewater of the printing and dyeing industry is added to the crushing and mixing mechanism 2 through the feed port 1, and the soil is crushed by the crushing wheel 3. The expanded culture enters the inoculation stirring mechanism 8 through the delivery pump I 12 and is mixed with the crushed soil. After the stirring paddle stirs and mixes, it is discharged through the material outlet I 13 and left to stand for 72 hours; the degradation rate of organic phosphorus, organic chloride and toluene in the discharged soil reaches 98.33%, 99.12% and 99.34% respectively. Figure 2 ).

[0030] Example 2: The device of this example is the same as that of Example 1, except that the magnetic electrode plate is made by grinding the magnetic carbon silicon to a particle size of less than 300 meshes, placing the crushed magnetic carbon in an ethanol solution with a mass concentration of 20% to prepare a 0.2 g / mL magnetic carbon silicon-ethanol mixture, stirring and mixing at 600 rpm, adding a PANI adhesive, the concentration of the PANI adhesive in the ethanol solution is 0.2 g / mL, stirring at 600 rpm for 10 minutes, and then adding a rare earth alloy ground to less than 300 meshes. The concentration of rare earth alloy in ethanol solution is 0.2g / mL, and the mixture is stirred and mixed for 60min at 50℃ to obtain a mixed slurry, which is placed under a roller mill and repeatedly rolled into a sheet solid with a thickness of 3cm, and then dried to obtain; the pore size of the magnetic electrode plate is 3mm, and the pore density is 15ppi; the inclination angle of the inclined bottom plate is 15°; the expansion culture medium is an aqueous solution containing 3% glucose, 2% bean cake powder, 1% corn powder, 0.5% yeast powder, 0.1% potassium dihydrogen phosphate, 0.2% calcium carbonate, 0.05% magnesium sulfate, and 0.05% vitamins, with a pH of 5.0; the added interferences include iron powder 0.15g / mL, KH2PO4 0.15mol / L, C14 H9Cl5 0.15 mol / L, benzene 0.15 mol / L, pH 6.0; the spray angle of the sprayer is 150°, and the spray flow rate is 11 L / min;

[0031] The above device was used to treat soil contaminated by textile industry wastewater. The organic phosphorus content in the soil was 1344 mg / L, the organic chloride content was 34.78 mg / L, and the toluene content was 17.98 mg / L.

[0032] The water containing the added interference substance enters the electromagnetic acclimation box 19 from the interference substance preparation tank 22 through the delivery pump IV 21, and is fully mixed with the soil polluted by textile industry wastewater for 2 years under the action of the toothed turntable II 26. The electric field voltage is 10V, and the soil microorganisms are acclimated under the electromagnetic action. After acclimation for 50 hours, the soil capable of treating organic pollutant microorganisms is obtained. The soil enters the expansion culture tank 14 through the delivery pump III 16 for expansion culture at 35°C; at the same time, the soil polluted by the wastewater of the printing and dyeing industry is added to the crushing and mixing mechanism 2 through the feed port 1, and the soil is crushed by the crushing wheel 3. The expanded culture enters the inoculation stirring mechanism 8 through the delivery pump I 12 and is mixed with the crushed soil. After the stirring paddle stirs and mixes, it is discharged through the material outlet I 13 and left to stand for 72 hours; after being treated with electromagnetic acclimation microorganisms for 72 hours, the organic phosphorus degradation rate reaches 99.38%, the organic chloride degradation rate reaches 99.47%, and the toluene degradation rate reaches 99.29% ( Figure 3 ).

[0033] Example 3: The device of this example is the same as that of Example 1, except that the magnetic electrode plate is used to grind graphene to a particle size of less than 300 meshes, and the crushed magnetic carbon is placed in an ethanol solution with a mass concentration of 15% to prepare a 0.3 g / mL graphene-ethanol mixture. After stirring and mixing at 1000 rpm, a PANI adhesive is added, and the concentration of the PANI adhesive in the ethanol solution is 0.7 g / mL. After stirring at 1000 rpm for 10 minutes, a nickel-based alloy ground to less than 300 meshes is added. The concentration of the nickel-based alloy in the ethanol solution is 0.4 g / mL, and the mixture is stirred and mixed for 60 min at 70°C to obtain a mixed slurry, which is placed under a roller mill and repeatedly rolled into a sheet solid with a thickness of 3 cm, and then dried to obtain the obtained product; the pore size of the magnetic electrode plate is 8 mm, and the pore density is 20 ppi; the inclination angle of the inclined bottom plate is 25°; the expansion culture medium is an aqueous solution containing 3% glucose, 2% bean cake powder, 1% corn powder, 0.5% yeast powder, 0.1% potassium dihydrogen phosphate, 0.2% calcium carbonate, 0.05% magnesium sulfate, and 0.05% vitamins, with a pH of 5.0; the added interferences include iron powder 0.2 g / mL, KH2PO4 0.5 mol / L, hexachlorocyclohexane 0.5 mol / L, ethylbenzene 0.5 mol / L, and the pH is 6.0; the spray angle of the sprayer is 120°, and the spray flow rate is 5 L / min;

[0034] The raw material in this embodiment is soil contaminated by wastewater from the pharmaceutical industry, and the organic phosphorus content in the soil is 1450 mg / L, the organic chloride content is 34.8 mg / L, and the toluene content is 27.85 mg / L.

[0035] The water containing the added interference substance enters the electromagnetic acclimation box 19 from the interference substance preparation tank 22 through the delivery pump IV 21, and is fully mixed with the soil contaminated by textile industry wastewater for 2 years under the action of the toothed turntable II 26. An external electric field voltage of 20V is applied to acclimate the soil microorganisms under the electromagnetic action. After acclimation for 55 hours, the soil capable of treating organic pollutant microorganisms is obtained. The soil enters the expansion culture tank 14 through the delivery pump III 16 for expansion culture at 40°C; at the same time, the soil contaminated by the wastewater of the printing and dyeing industry is added to the crushing and mixing mechanism 2 through the feed port 1, and the soil is crushed by the crushing wheel 3. The expanded culture enters the inoculation stirring mechanism 8 through the delivery pump I 12 and is mixed with the crushed soil. After the stirring paddle stirs and mixes, it is discharged through the material outlet I 13 and left to stand for 72 hours. After being treated with electromagnetic acclimated microorganisms for 72 hours, the organic phosphorus degradation rate reaches 98.92%, the organic chloride degradation rate reaches 99.17%, and the toluene degradation rate reaches 99.79%.

Claims

1. A device for multi-field enhanced biological treatment of soil contaminated by organophosphorus, organochlorine and benzene series, characterized by: It includes a crushing and mixing mechanism (2), an electromagnetic taming mechanism, an expansion culture mechanism, and an inoculation and stirring mechanism (8); The crushing and mixing mechanism comprises a shell, the top of which is provided with a feed inlet (1), a crushing wheel (3) is installed in the shell, and a toothed turntable I (4) is provided at the bottom of the shell; the crushing and mixing mechanism is connected to the inoculation and stirring mechanism (8) through a spiral feeding trough (5); the inoculation and stirring mechanism (8) is provided with a stirring paddle (9) driven by a stepper (6), the lower end of the stirring paddle (9) is movably arranged on a stirring shaft bracket (10), a plurality of sprayers (7) are arranged at the top of the inoculation and stirring mechanism (8), the sprayers (7) are connected to the expansion culture tank (14) through a delivery pump I (12), the top of the inoculation and stirring mechanism (8) is provided with an exhaust gas outlet (11), and the bottom of the inoculation and stirring mechanism is provided with a material outlet I (13); the expansion culture mechanism comprises a culture medium configuration tank (15) and an expansion culture tank (14), the culture medium configuration tank (15) is provided with a solvent inlet I (18) and a solute inlet I (25), and the bottom of the culture medium configuration tank is provided with a material outlet I (13). The outlet is connected to the expansion culture tank (14) through a delivery pump II (17), the top of the expansion culture tank (14) is connected to the electromagnetic acclimation box (19) through a delivery pump III (16), and the culture medium configuration tank (15) and the expansion culture tank (14) are both provided with a spiral stirrer driven by a stepping motor; the electromagnetic acclimation mechanism comprises an electromagnetic acclimation box (19) and an interfering substance configuration tank (22), the interfering substance configuration tank (22) is provided with a solvent inlet II (23) and a solute inlet II (24), magnetic electrode plates (20) are provided on both sides of the electromagnetic acclimation box, and the magnetic electrode plates are respectively connected to the positive and negative poles of the power supply (28), an inclined toothed turntable II (26) is provided at the bottom of the electromagnetic acclimation box, a material outlet II (27) is opened at the bottom of the electromagnetic acclimation box, the toothed turntable II (26) is adjacent to the material outlet II (27), and the interfering substance configuration tank (22) is connected to the electromagnetic acclimation box (19) through a delivery pump IV (21).

2. The device for multi-field enhanced biological treatment of soil contaminated by organophosphorus, organochlorine and benzene series substances according to claim 1, characterized in that: The spray angle of the sprayer (7) in the inoculation stirring mechanism (8) is 15° to 180°, and the spray flow rate is 0.2 to 15 L / min. The sprayed liquid culture solution is fully mixed with the material after being stirred by the stirring paddle (9).

3. The device for multi-field enhanced biological treatment of soil contaminated by organophosphorus, organochlorine and benzene series substances according to claim 1, characterized in that: The magnetic electrode plate is prepared by grinding a conductive material to a particle size of less than 300 meshes, placing the crushed conductive material in an ethanol solution with a mass concentration of 15% to 60% to obtain a 0.1 to 0.4 g / mL conductive material-ethanol mixture, stirring and mixing at 400 to 1500 rpm, adding a PANI adhesive, the concentration of the PANI adhesive in the ethanol solution is 0.1 to 0.8 g / mL, stirring at 400 to 1500 rpm for 10 to 15 minutes, adding a magnetic material ground to less than 300 meshes, the concentration of the magnetic material in the ethanol solution is 0.1 to 0.4 g / mL, continuing to stir and mix at 40 to 80° C. for 40 to 80 minutes to obtain a mixed slurry, placing the obtained mixed slurry under a double-roller machine, repeatedly rolling it into a flaky solid with a thickness of 0.1 to 10 cm, and drying it to obtain the magnetic electrode plate.

4. The device for multi-field enhanced biological treatment of soil contaminated by organophosphorus, organochlorine and benzene series substances according to claim 3 is characterized by: The conductive material is one of magnetic carbon, magnetic carbon silicon, graphene, carbon nanotube, fullerene, silver and copper.

5. The device for multi-field enhanced biological treatment of soil contaminated by organophosphorus, organochlorine and benzene series substances according to claim 3, characterized in that: The magnetic material is one of electrical steel, rare earth alloy, nickel-based alloy and iron oxide material.

6. The device for multi-field enhanced biological treatment of soil contaminated by organophosphorus, organochlorine and benzene series substances according to claim 3, characterized in that: The magnetic electrode plate is provided with holes, the hole diameter is 0.1~20mm, the pore density is 1~50ppi, and the applied electric field voltage is 0.2~35V.

7. The device for multi-field enhanced biological treatment of soil contaminated by organophosphorus, organochlorine and benzene series substances according to claim 3, characterized in that: The inclination angle of the inclined bottom plate of the electromagnetic taming box (19) is 5° to 35°.

8. The method for using the multi-field enhanced biological treatment device for soil contaminated by organophosphorus, organochlorine and benzene series substances as claimed in claim 1, characterized in that: Water containing added interference substances enters the electromagnetic acclimation box (19) from the interference substance preparation tank through the delivery pump IV (21), and is fully mixed with soil containing organophosphorus, organochlorine and benzene series that has been contaminated for more than one year under the action of the toothed turntable II (26). After electromagnetic acclimation, soil containing functional microorganisms is obtained. The soil enters the expansion culture tank (14) through the delivery pump III (16) for expansion culture; the soil contaminated with organophosphorus, organochlorine and benzene series is added to the crushing and mixing mechanism from the feed inlet, and the soil is crushed by the crushing wheel. The expanded culture enters the inoculation stirring mechanism (8) through the delivery pump I (12) and is fully mixed with the soil contaminated with organophosphorus, organochlorine and benzene series, and the mixed material is discharged through the material outlet I (13).

9. The method of use according to claim 8, characterized in that: The added interference substances are selected from iron powder, KH2PO4, carbon tetrachloride, C 14 H9Cl5, hexachlorocyclohexane, benzene, toluene, ethylbenzene, and xylene, their concentrations in water are 0.05~0.75mol / L, and the pH range is 4.5~8.5.

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