A supramolecular peony peptide composition and preparation method thereof

Abstract

The present invention discloses a supramolecular peony peptide composition and a preparation method thereof, and belongs to the field of daily chemical products. The preparation method of the supramolecular peony peptide composition of the present invention uses a supramolecular solvent to synergistically promote the penetration of key active substances, peony branch / flower / leaf extract and acetyl hexapeptide-8, so that the resulting product has nano-level active particles, thereby improving the rapid skin permeability of the overall product. The present invention also discloses a supramolecular peony peptide composition prepared by the method, which takes into account both hydrophilicity and lipophilicity, can quickly penetrate into the deep layer of the skin and fully demonstrate good wrinkle removal, anti-oxidation and soothing effects. The present invention also discloses cosmetics prepared by further application of the supramolecular peony peptide composition.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemical products, and in particular to a supramolecular peony peptide composition and a preparation method thereof. Background Art

[0002] The aging of the human body will show a slowing of the metabolic rate and a decrease in the moisturizing factors in the dermis, which will lead to poor skin moisturizing ability, reduced elasticity, and wrinkles. For this reason, people are focusing on the development of skin care products and cosmetics with anti-aging functions. In these products, peptides are widely used as key components due to their higher biological activity.

[0003] However, in the current skin care products and cosmetics containing peptides, it is difficult for peptides to fully exert their multi-effects (including anti-wrinkle, anti-oxidation and soothing effects) because peptides have poor penetration ability in the skin surface, strong hydrophilicity and weak lipophilicity in the body, and therefore the overall bioavailability is not high. Summary of the invention

[0004] Based on the defects of the prior art, the purpose of the present invention is to provide a method for preparing a supramolecular peony peptide composition, which uses a supramolecular solvent to synergistically promote the penetration of key active substances, peony branch / flower / leaf extract and acetyl hexapeptide-8, so that the resulting product has nano-scale active particles, thereby improving the rapid skin permeability of the overall product; the supramolecular peony peptide composition takes into account both hydrophilicity and lipophilicity, and can fully demonstrate good wrinkle removal, antioxidant and soothing effects.

[0005] A method for preparing a supramolecular peony peptide composition comprises the following steps:

[0006] (1) mixing and heating an ionic liquid solvent under a protective gas atmosphere and performing an ion exchange reaction, followed by primary impurity removal, homogenization, secondary impurity removal and purification to obtain a supramolecular solvent; the ionic liquid comprises at least two of betaine, malic acid, citric acid and alkyl glucoside;

[0007] (2) Under a protective gas atmosphere, the supramolecular solvent is heated, and then the peony (PAEONIA SUFFRUTICOSA) branch / flower / leaf extract and acetyl hexapeptide-8 are added under shear conditions, and the supramolecular peony peptide composition is obtained after high-pressure homogenization or microfluidization treatment.

[0008] Generally speaking, since the epidermis of the skin of an organism is hydrophilic, and the stratum corneum is lipophilic, the polypeptides in traditional polypeptide-containing products are difficult to penetrate quickly and deeply. Although the permeability of the overall product can be improved by relying on amphiphilic solvents, the polypeptides are easily separated from the solvent during the penetration process, resulting in loss of activity and waste of active substances. Therefore, in the technical solution of the present invention, the inventor uses a supramolecular solvent prepared by an ionic liquid to synergistically promote the penetration of key active substances, peony branch / flower / leaf extract and acetyl hexapeptide-8, to form supramolecular peptides. The surface electrostatic redistribution of the product is different from the raw materials. The positive potential and negative potential of the product surface are gathered separately, so that the molecules attract each other under the action of Coulomb force and maintain stability; at the same time, the positive potential end of the product is lipophilic, and the negative potential end is hydrophilic, so the supramolecular peptide composition can be smoothly entered by the affinity end based on the principle of similar dissolving when crossing the stratum corneum or epidermis, and then the non-affinity section is dragged in by the Coulomb force, so as to achieve the penetration effect in different skin layers.

[0009] In addition, among the key active material raw materials used in the scheme of the present invention, acetyl hexapeptide-8, also known as hexapeptide, is a small molecule composed of six amino acids. Its mechanism of action is similar to that of botulinum toxin. During the aging of the skin, nerve endings release neurotransmitters (acetylcholine) to induce muscle relaxation, and acetyl hexapeptide-8 can participate in the competition for SNAP-25 at the site of the melt-vesicle complex, affecting the formation of the complex. When the complex is unstable, the vesicle cannot effectively release neurotransmitters, thereby weakening muscle contraction, relaxing the facial muscles caused by nervous tension, and preventing the formation of wrinkles. It has an excellent effect in diluting dynamic lines around the eyes. The peony branch / flower / leaf extract is extracted by fermenting the whole peony plant with plant lactobacillus, and is rich in flavonoids, polyphenols and other active ingredients. It can not only effectively remove free radicals in the initiation stage of the chain reaction, but also directly capture free radicals in the free radical reaction chain, block the free radical chain reaction, play a dual role of prevention and chain breaking, and has the effect of delaying aging. After being fully acted upon by the supramolecular solvent, these two raw materials will generate highly dispersible nano-scale small molecule peptide particles in the solvent. These particles have higher penetration efficiency. After entering deep into the skin, these active substances can fully exert their antioxidant and anti-wrinkle effects, while also having skin soothing and anti-inflammatory effects.

[0010] It should also be noted that the peony branch / flower / leaf extract described in the present invention can be a homemade product or a commercially purchased product. Those skilled in the art should know that the peony branch / flower / leaf extract obtained without a special process has similar initial properties.

[0011] Preferably, in the supramolecular peony peptide composition, the mass content of the peony branch / flower / leaf extract is 0.0001 to 10 wt %, and the mass content of acetyl hexapeptide-8 is 0.01 to 1 wt %.

[0012] More preferably, in the supramolecular peony peptide composition, the mass content of the peony branch / flower / leaf extract is 2.5-7.5wt%, and the mass content of acetyl hexapeptide-8 is 0.25-0.75wt%.

[0013] According to experiments conducted by the inventors, when one of the peony branch / flower / leaf extract and acetyl hexapeptide-8 is missing during the preparation of the product, the permeability of the product will be greatly reduced. However, with the introduction and increase of the peony branch / flower / leaf extract and acetyl hexapeptide-8, the permeability of the prepared supramolecular peony peptide composition will first be significantly improved, and then begin to decline after reaching a certain peak value. The comprehensive permeability of the product prepared within the above range is the best.

[0014] Preferably, the ionic liquid solvent is a mixture of betaine and malic acid, and the mass ratio of betaine to malic acid is 16:(0.5-1.5).

[0015] Malic acid is a fruit acid. When acting on the skin, it can accelerate the shedding of keratinocytes, soften the stratum corneum, increase skin collagen, and effectively improve the phenomenon of dry skin. At the same time, it is also a natural moisturizing ingredient that can remove wrinkles on the skin surface and achieve anti-aging effects. Betaine exists naturally in many plants. Modern research shows that betaine has the ability to quickly improve skin moisture retention, has unique moisturizing and cell membrane protection properties, and can increase the solubility of active ingredients in water and reduce the irritation of surfactants or fruit acids to the skin. When these two ionic liquids are mixed as supramolecular solvents to prepare products, they can also play their own skin care effects, further improving the skin care effect of the product.

[0016] Preferably, the temperature of the ion exchange reaction in step (1) is 20 to 100° C. and the time is 4 to 72 hours.

[0017] Preferably, the solvent used for the primary and secondary impurity removal in step (1) is ethanol, and the secondary impurity removal is performed under an ultrasonic environment.

[0018] Preferably, the purification in step (1) is carried out using a vacuum rotary evaporator.

[0019] Preferably, the speed under the shearing condition in step (2) is 200 to 1000 rpm.

[0020] Preferably, in step (2), the temperature of the supramolecular solvent after heating is ≤45°C.

[0021] Preferably, the conditions for high-pressure homogenization in step (2) are: homogenization pressure 400-1500 bar, homogenization times 2-10 times, homogenization temperature ≤80°C; the conditions for microfluidization treatment are: working pressure 400-500 bar, working temperature 25-35°C.

[0022] Another object of the present invention is to provide a supramolecular peony peptide composition prepared by the preparation method of the supramolecular peony peptide composition.

[0023] The supramolecular peony peptide composition of the present invention has good hydrophilic and lipophilic amphiphilicity, and can effectively and quickly penetrate deep into the skin, thereby achieving good skin care function; at the same time, this effect is not comparable to any product prepared from any existing natural raw materials. The product of the present invention has a significant improvement in technical effect compared to the same type of products in the prior art.

[0024] Another object of the present invention is to provide a cosmetic comprising the supramolecular peony peptide composition of the present invention.

[0025] When the supramolecular peony peptide composition of the present invention is used as a cosmetic ingredient, it not only has strong permeability, but also can fully exert the skin care effects of the active ingredients peony branch / flower / leaf extract and acetyl hexapeptide-8. At the same time, the supramolecular solvent contained in it can also cooperate with the solvent components in the cosmetics to further improve the stability of the product and the absorption activity in the skin, so that the product exhibits excellent antioxidant, soothing, anti-inflammatory and anti-wrinkle effects.

[0026] Preferably, the dosage form of the cosmetic is any one of a liquid or a solid.

[0027] More preferably, the cosmetic is any one of an aqueous cosmetic, an emulsion cosmetic, or a paste cosmetic.

[0028] Preferably, in the cosmetic, the mass content of the supramolecular peony peptide composition is 1 to 10 wt%.

[0029] More preferably, the cosmetic components, calculated as 100 parts, include the following components by weight:

[0030] 4-6 parts of glycerin, 3-5 parts of butylene glycol, 0.1-0.5 parts of carbomer, 0.01-0.1 parts of xanthan gum, 0.1-1 parts of p-hydroxyacetophenone, 0.01-0.1 parts of sodium gluconate, 0.1-0.5 parts of olive oil emulsifying wax, 2-4 parts of dicaprylyl carbonate, 0.5-2 parts of behenyl alcohol, 0.1-1 parts of 1,2-hexanediol, 0.1-0.5 parts of arginine, 1-10 parts of supramolecular peony peptide composition and the balance of water.

[0031] The supramolecular peony peptide composition of the present invention has high solvent compatibility and component matching stability, and the cosmetics prepared with the components can give full play to the synergistic skin care effects of the polypeptide active substance of the supramolecular peony peptide composition and other active components, and the product performance is excellent.

[0032] Another object of the present invention is to provide a method for preparing the cosmetic, comprising the following steps:

[0033] (1) mixing glycerin, butylene glycol, carbomer, xanthan gum, p-hydroxyacetophenone, sodium gluconate and water, and then heating and stirring to obtain a mixture A, and keeping the mixture A warm;

[0034] (2) mixing olive oil emulsified wax, dicaprylyl carbonate, and behenyl alcohol and heating until the resulting solution turns yellow to obtain a mixture B;

[0035] (3) Mixing mixture A and mixture B homogenously and cooling to obtain mixture C;

[0036] (4) 1,2-hexanediol, arginine and the supramolecular peony peptide composition are sequentially added to the mixture C and mixed evenly, and sieved to obtain the cosmetic.

[0037] The beneficial effect of the present invention is that the present invention provides a method for preparing a supramolecular peony peptide composition, which uses a supramolecular solvent to synergistically promote the penetration of key active substances, peony branch / flower / leaf extract and acetyl hexapeptide-8, so that the resulting product has nano-level active particles, thereby improving the rapid skin permeability of the overall product. The present invention also provides a supramolecular peony peptide composition prepared by the method, which takes into account both hydrophilicity and lipophilicity, can quickly penetrate into the deep layer of the skin and fully demonstrate good wrinkle removal, anti-oxidation and soothing effects. The present invention also provides cosmetics prepared by further application of the supramolecular peony peptide composition. DETAILED DESCRIPTION

[0038] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments and comparative examples, the purpose of which is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present invention. The experimental reagents and instruments involved in the implementation of the present invention, unless otherwise specified, are commonly used common reagents and instruments. The peony branch / flower / leaf extract used in the embodiments of the present invention and the comparative examples is a commercially available product.

[0039] Example 1

[0040] An embodiment of the supramolecular peony peptide composition and the preparation method thereof of the present invention comprises the following steps:

[0041] (1) betaine and malic acid are mixed in a homogenizer at a mass ratio of 16:1 under an inert gas atmosphere, heated to 60° C., and subjected to ion exchange reaction for 24 hours, cooled to room temperature, and then washed with ethanol once to remove impurities, homogenized, and removed impurities with ethanol twice under an ultrasonic environment for 20 minutes, and then purified by a vacuum rotary evaporator to remove ethanol to obtain a supramolecular solvent;

[0042] (2) Under an inert gas atmosphere, the supramolecular solvent is heated, and then the peony branch / flower / leaf extract and acetyl hexapeptide-8 are added under a shearing condition of 500 rpm, and the supramolecular peony peptide composition is obtained after high-pressure homogenization; the conditions of the high-pressure homogenization are: homogenization pressure 800 bar, homogenization times 5 times, and homogenization temperature ≤80°C; in the supramolecular peony peptide composition, the mass content of the peony branch / flower / leaf extract is 0.0001wt%, and the mass content of acetyl hexapeptide-8 is 0.01wt%.

[0043] Example 2

[0044] An embodiment of the supramolecular peony peptide composition and the preparation method thereof of the present invention is different from Example 1 only in that, in the supramolecular peony peptide composition, the mass content of the peony branch / flower / leaf extract is 2.5wt%, and the mass content of acetyl hexapeptide-8 is 0.25wt%.

[0045] Example 3

[0046] An embodiment of the supramolecular peony peptide composition and preparation method thereof of the present invention is different from Example 1 only in that, in the supramolecular peony peptide composition, the mass content of peony branch / flower / leaf extract is 5wt%, and the mass content of acetyl hexapeptide-8 is 0.5wt%.

[0047] Example 4

[0048] An embodiment of the supramolecular peony peptide composition and the preparation method thereof of the present invention is different from Example 1 only in that, in the supramolecular peony peptide composition, the mass content of the peony branch / flower / leaf extract is 7.5wt%, and the mass content of acetyl hexapeptide-8 is 0.75wt%.

[0049] Example 5

[0050] An embodiment of the supramolecular peony peptide composition and the preparation method thereof of the present invention is different from Example 1 only in that, in the supramolecular peony peptide composition, the mass content of the peony branch / flower / leaf extract is 10wt%, and the mass content of acetyl hexapeptide-8 is 1wt%.

[0051] Example 6

[0052] An embodiment of the supramolecular peony peptide composition and preparation method thereof of the present invention is different from Embodiment 2 only in that malic acid is replaced by citric acid.

[0053] Comparative Example 1

[0054] A supramolecular peony peptide composition and a preparation method thereof, which differs from Example 5 only in that, in the supramolecular peony peptide composition, the mass content of peony branch / flower / leaf extract is 0wt%, and the mass content of acetyl hexapeptide-8 is 1wt%.

[0055] Comparative Example 2

[0056] A supramolecular peony peptide composition and a preparation method thereof, which differs from Example 5 only in that, in the supramolecular peony peptide composition, the mass content of peony branch / flower / leaf extract is 10wt%, and the mass content of acetyl hexapeptide-8 is 0wt%.

[0057] Comparative Example 3

[0058] A supramolecular white truffle peptide composition and a preparation method thereof, which are different from those of Example 5 only in that, in the supramolecular white truffle peptide composition, the peony branch / flower / leaf extract is replaced by a commercially available white truffle (TUBERMAGNATUM) extract.

[0059] Comparative Example 4

[0060] A peony peptide composition and a preparation method thereof, the preparation method comprising the following steps:

[0061] Under an inert gas atmosphere, betaine and malic acid are mixed in a homogenizer at a mass ratio of 16:1 and heated to 60° C., and then peony branch / flower / leaf extract and acetyl hexapeptide-8 are added under a shearing condition of 500 rpm, and the peony peptide composition is obtained after high-pressure homogenization; the conditions of the high-pressure homogenization are the same as those in Example 1; in the peony peptide composition, the mass content of the peony branch / flower / leaf extract is 0.0001wt%, and the mass content of acetyl hexapeptide-8 is 0.01wt%.

[0062] Comparative Example 5

[0063] A peony peptide composition and a preparation method thereof. The preparation method is the same as that of comparative example 4. In the peony peptide composition, the mass content of peony branch / flower / leaf extract is 2.5wt%, and the mass content of acetyl hexapeptide-8 is 0.25wt%.

[0064] Comparative Example 6

[0065] A peony peptide composition and a preparation method thereof. The preparation method is the same as that of comparative example 4. In the peony peptide composition, the mass content of peony branch / flower / leaf extract is 5wt%, and the mass content of acetyl hexapeptide-8 is 0.5wt%.

[0066] Comparative Example 7

[0067] A peony peptide composition and a preparation method thereof. The preparation method is the same as that of comparative example 4. In the peony peptide composition, the mass content of peony branch / flower / leaf extract is 7.5wt%, and the mass content of acetyl hexapeptide-8 is 0.75wt%.

[0068] Comparative Example 8

[0069] A peony peptide composition and a preparation method thereof. The preparation method is the same as that of comparative example 4. In the peony peptide composition, the mass content of peony branch / flower / leaf extract is 10wt%, and the mass content of acetyl hexapeptide-8 is 1wt%.

[0070] Comparative Example 9

[0071] A peony peptide composition and a preparation method thereof. The preparation method is the same as that of comparative example 4. In the peony peptide composition, the mass content of peony branch / flower / leaf extract is 0wt%, and the mass content of acetyl hexapeptide-8 is 1wt%.

[0072] Comparative Example 10

[0073] A peony peptide composition and a preparation method thereof. The preparation method is the same as that of comparative example 4. In the peony peptide composition, the mass content of peony branch / flower / leaf extract is 10wt%, and the mass content of acetyl hexapeptide-8 is 0wt%.

[0074] Comparative Example 11

[0075] A white truffle peptide composition and a preparation method thereof. The preparation method is the same as that of comparative example 4. In the white truffle peptide composition, the peony branch / flower / leaf extract is replaced with a commercially available white truffle extract.

[0076] Effect Example 1

[0077] In order to verify the skin penetration effect of the supramolecular peony peptide composition of the present invention, the following transdermal test experiment was performed:

[0078] The commercially available pig skin model was used to detect the transdermal permeation of the product at different times and evaluate the transdermal delivery efficiency of the main components in the sample. The in vitro transdermal diffusion experiment can predict the rate of transdermal absorption of the product. The skin is composed of the stratum corneum, epidermis, dermis, subcutaneous tissue, etc. After the product is placed on the skin surface, it penetrates into the skin and reaches the dermis through the epidermis. Since there are abundant capillaries in the dermis, the product can be quickly absorbed into the systemic circulation. Therefore, the concentration of the product on the inner surface of the skin is very low, which meets the so-called "leakage trough" condition, and the concentration of the product is close to 0. The skin is fixed on the Franz diffusion cell, with the stratum corneum facing the drug administration pool and the dermis facing the absorption chamber, and the product is placed in the drug administration pool. The product concentration in the medium in the receiving pool on the other side of the skin is measured at given time intervals to analyze the kinetics of drug permeation through the skin.

[0079] Specific steps:

[0080] (1) Microscopic examination: Select undamaged pig skin under a dissecting microscope, cut several pieces of skin of the same size, wash them once with physiological saline, and dry the surface moisture with filter paper;

[0081] (2) Fixing the skin: Fix the skin on the Franz diffusion cell with the stratum corneum facing the dosing chamber and the dermis facing the receiving chamber. Add 15 ml of normal saline into the receiving chamber and remove bubbles to ensure that there are no bubbles between the dermis and the receiving solution.

[0082] (3) Drug administration: Turn on the instrument in advance, adjust the water bath temperature to 37±1°C, add 1 mL of the products of different embodiments and comparative examples into the drug administration chamber, seal with sealing film, and add tin foil to prevent liquid evaporation, where the effective penetration area is 0.36πcm 2 ;

[0083] (4) Infiltration: set stirring at 300 rpm / min;

[0084] (5) Sampling: At specific time points (1 h, 3 h, 5 h, and 7 h), the skin was cut into pieces and extracted. After filtration using a 0.22 μm organic membrane, HPLC detection was performed and the percutaneous permeation amount was calculated.

[0085] The test results are shown in Table 1.

[0086] Table 1

[0087]

[0088]

[0089] As can be seen from Table 1, the supramolecular peony peptide composition prepared by the method of the present invention has excellent skin permeability. This effect comes from the product obtained by the supramolecular solvent synergistically promoting the penetration of two key raw materials. When one of the two key raw materials is missing or replaced with other raw materials with similar effects, as shown in Comparative Examples 1 to 3, it is difficult to exert the expected penetration effect; and the two raw materials are treated by ordinary processes. Even if the types and addition amounts of each additive do not change, the prepared product does not have obvious amphiphilicity. As shown in Comparative Examples 4 to 11, even if the addition amounts of the two key raw materials are adjusted, or the types of key raw materials are replaced, the permeation amount of the product after 7 hours is only about one-fifteenth to one-twentieth of the products of each embodiment. In the comparison of the products of each embodiment, it can be found that when the mass content of the peony branch / flower / leaf extract in the supramolecular peony peptide composition is 2.5-7.5wt%, and the mass content of acetyl hexapeptide-8 is 0.24-0.75wt%, the skin permeation amount of the product is the highest and the permeation effect is the best.

[0090] Effect Example 1

[0091] In order to verify the actual use effect of the supramolecular peony peptide composition of the present invention, the products of Example 3 and Comparative Example 6 were used to prepare cosmetics according to the formula described in Table 2 using the following steps:

[0092] (1) Glycerin, butylene glycol, carbomer, xanthan gum, p-hydroxyacetophenone, sodium gluconate and water are put into an emulsifying pot and mixed and homogenized until there are no large particles, and then heated to 80-85° C. and stirred until the material body has a translucent effect of a certain consistency to obtain a mixture A, and the mixture A is kept warm for 30 minutes;

[0093] (2) Mix olive oil emulsified wax, dicaprylyl carbonate, and behenyl alcohol in an oil pan and heat at 80-85° C. until the resulting solution turns yellow to obtain a mixture B;

[0094] (3) Mixing mixture A and mixture B for 5 to 10 minutes to form a white paste, and cooling to 40 to 45° C. to obtain mixture C;

[0095] (4) 1,2-hexanediol, arginine and the supramolecular peony peptide composition (or peony peptide composition) are sequentially added to the mixture C and mixed evenly, and the mixture is sieved through a 200-mesh filter cloth to obtain the cosmetic.

[0096] Table 2

[0097]

[0098] Each product was subjected to (1) soothing test; (2) anti-wrinkle test; and (3) antioxidant test.

[0099] The test steps of the relief test are:

[0100] Use a 96-well blackboard to set up control wells and sample wells, and add samples and solutions (unit: μL) in sequence according to Table 3. After adding the sample to be tested, mix well and incubate at 37°C for 10 minutes. After adding 5 microliters of COX-2Probe to each well, quickly add 5 μL of COX-2Substrate working solution to each well, mix well, incubate at 37°C in the dark for 30 minutes, and then perform fluorescence test and calculate according to the following formula.

[0101] Inhibition rate %=(RFU(100% enzyme activity control)-RFU(sample)) / (RFU(100% enzyme activity control)-RFU(blank control))*100%.

[0102] Table 3

[0103] Blank control 100% enzyme activity control Positive inhibitor control sample COX-2Assay Buffer 80 75 75 75 COX-2 Cofactor working fluid 5 5 5 5 COX-2 working solution - 5 5 5 Sample solvent 5 5 - - Celecoxib Solution - - 5 - Samples to be tested - - - 5

[0104] Test principle:

[0105] Cyclooxygenase (COX) is an enzyme that can produce signals that cause pain and inflammation. PGs are an important class of inflammatory mediators. In the inflammatory model, COX-2 mRNA and protein are significantly increased, increasing the production of PGs. During the inflammatory process, COX-2 is induced and expressed in large quantities, causing the amplification and enhancement of the inflammatory response, resulting in damage to the body. In the presence of a cofactor, COX-2 uses its cyclooxygenase (COX) activity to epoxidize substrates such as arachidonic acid to produce intermediates such as PGG2. Then, COX-2 uses its peroxidase activity to catalyze intermediates such as PGG2 to produce final products such as PGH2. At the same time, it catalyzes the COX-2 probe (Probe) with almost no fluorescence to produce a probe (Ex560 / Em590) with strong fluorescence. In this way, the enzyme activity of COX-2 can be detected very sensitively through fluorescence detection. If a COX-2 inhibitor (Inhibitor) is added to the reaction, the generation of fluorescence will be inhibited, and the fluorescence intensity is inversely proportional to the inhibitory effect of the inhibitor, thereby detecting the inhibitory effect of the inhibitor.

[0106] The test results are shown in Table 4:

[0107] Table 4

[0108]

[0109] It can be seen that the cosmetics prepared by the supramolecular peony peptide composition of the present invention have significantly improved soothing effect compared with the cosmetics prepared by the conventional peony peptide composition. As the content of the supramolecular peony peptide composition increases, the COX-2 activity inhibition rate can reach up to 75.12%, which is almost twice that of Comparative Example 14 prepared under the same ratio; and according to Comparative Example 15, except for the supramolecular peony peptide composition, the other components in the cosmetics do not have soothing and anti-inflammatory effects.

[0110] The test steps of the wrinkle removal test in this effect example are:

[0111] 70 volunteers aged 35-50 were recruited and randomly divided into 7 groups, 10 people in each group. Each group used the cosmetics of Examples 7-9 and Comparative Examples 12-15 for experiments, and used them once in the morning and evening every day. The area around the eyes of the volunteers was used as the test area. After cleansing, in a room with a room temperature of 22°C ± 2°C and a relative humidity of 40 ± 2%, wait for 15 minutes and then take a facial photo of the volunteer with a VISIA facial image analyzer. The analysis area should try to avoid interference from reflective areas and hair, record the initial value of the number of wrinkles A0, and take a photo to record the number of wrinkles A1 after one month of continuous use. Wrinkle number reduction rate = (A1-A0) / (A1+AO)*100%, take the average value of the eye wrinkle number reduction rate of each group of volunteers, and the results are shown in Table 5.

[0112] Table 5

[0113]

[0114]

[0115] As can be seen from Table 5, the cosmetics prepared from the supramolecular peony peptide composition of the present invention have an obvious wrinkle-removing effect, and as the content of the supramolecular peony peptide composition increases, the wrinkle-removing effect is significantly improved, and the effect is also better than similar products prepared from ordinary peony peptide compositions. However, it can be seen from Comparative Example 15 that in the cosmetics of the present invention, other ingredients except the supramolecular peony peptide composition have no obvious wrinkle-removing effect.

[0116] The test steps of the antioxidant test in this effect example are:

[0117] Set up a sample tube (T), sample background (T0), DPPH tube (C) and solvent background (C0). For each sample of Examples 7 to 9 and Comparative Examples 12 to 15, set up 3 parallel tubes for the sample tube (T). Add 1 mL of sample solution of the same concentration to each of the sample tubes (T) and sample background (T0). Make up to 2 mL in a test tube and mix well. Add 1 mL of DPPH ethanol solution to the sample tube (T) and DPPH tube (C), shake gently, let stand at room temperature for 5 minutes, transfer each reaction solution into a 1 cm cuvette, and measure the absorbance at 517 nm.

[0118] DPPH clearance rate (%) X = (1-(T-T0) / (C-C0))*100%

[0119] Test principle:

[0120] DPPH is a stable, long-lived free radical. Its ethanol solution is dark purple and has strong absorption near 517nm. When there is a free radical scavenger, the light absorption of the DPPH ethanol solution is weakened due to pairing with its single electron. The degree of fading of the DPPH ethanol solution is linearly related to the number of electrons it accepts, which can be used to evaluate the ability of the test sample to scavenge free radicals.

[0121] The test results are shown in Table 6.

[0122] Table 6

[0123] Group DPPH removal rate% Example 7 18.35 Example 8 24.12 Example 9 36.70 Comparative Example 12 -1.55 Comparative Example 13 -0.8 Comparative Example 14 2.17 Comparative Example 15 -1.43

[0124] It can be seen that the cosmetics prepared by the supramolecular peony peptide composition of the present invention have obvious antioxidant effect, and the DPPH scavenging rate can reach more than 35% as the content of the supramolecular peony peptide composition increases, while the cosmetics prepared by the ordinary peony peptide composition do not have this effect, and as the content of the peony peptide composition increases, the product does not show obvious changes in antioxidant effect.

[0125] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A method for preparing a supramolecular peony peptide composition, characterized in that: The following steps are involved: (1) mixing and heating an ionic liquid solvent under a protective gas atmosphere and performing an ion exchange reaction, followed by primary impurity removal, homogenization, secondary impurity removal and purification to obtain a supramolecular solvent; the ionic liquid comprises at least two of betaine, malic acid and citric acid; the solvent used for the primary impurity removal and the secondary impurity removal is ethanol, and the secondary impurity removal is performed under an ultrasonic environment; (2) Under a protective gas atmosphere, the supramolecular solvent is heated, and then the peony branch / flower / leaf extract and acetyl hexapeptide-8 are added under shear conditions, and the supramolecular peony peptide composition is obtained after high-pressure homogenization or microfluidization treatment.

2. The method for preparing the supramolecular peony peptide composition according to claim 1, characterized in that: The ionic liquid solvent is a mixture of betaine and malic acid, and the mass ratio of betaine to malic acid is 16:(0.5-1.5).

3. The method for preparing the supramolecular peony peptide composition according to claim 1, characterized in that: Include at least one of the following (a) to (e): (a) The temperature during the ion exchange reaction in step (1) is 20 to 100° C. and the time is 4 to 72 hours; (b) the purification in step (1) is carried out using a vacuum rotary evaporator; (c) the speed under the shearing condition in step (2) is 200 to 1000 rpm; (d) the temperature of the supramolecular solvent after heating in step (2) is ≤ 45°C; (e) The conditions for high pressure homogenization in step (2) are: homogenization pressure 400-1500 bar, homogenization times 2-10 times, homogenization temperature ≤80°C; the conditions for microfluidization treatment are: working pressure 400-500 bar, working temperature 25-35°C.

4. The supramolecular peony peptide composition prepared by the method for preparing the supramolecular peony peptide composition according to any one of claims 1 to 3.

5. The supramolecular peony peptide composition according to claim 4, characterized in that: In the supramolecular peony peptide composition, the mass content of the peony branch / flower / leaf extract is 0.0001-10wt%, and the mass content of acetyl hexapeptide-8 is 0.01-1wt%.

6. The supramolecular peony peptide composition according to claim 5, characterized in that: In the supramolecular peony peptide composition, the mass content of the peony branch / flower / leaf extract is 2.5-7.5wt%, and the mass content of acetyl hexapeptide-8 is 0.25-0.75wt%.

7. A cosmetic, characterized in that: The components include the supramolecular peony peptide composition according to any one of claims 4 to 6.

8. The cosmetic according to claim 7, characterized in that: In the cosmetic, the mass content of the supramolecular peony peptide composition is 1 to 10 wt %.

9. The cosmetic according to claim 8, characterized in that: The cosmetic composition, calculated as 100 parts, includes the following components by weight: 4-6 parts of glycerin, 3-5 parts of butylene glycol, 0.1-0.5 parts of carbomer, 0.01-0.1 parts of xanthan gum, 0.1-1 parts of p-hydroxyacetophenone, 0.01-0.1 parts of sodium gluconate, 0.1-0.5 parts of olive oil emulsifying wax, 2-4 parts of dicaprylyl carbonate, 0.5-2 parts of behenyl alcohol, 0.1-1 parts of 1,2-hexanediol, 0.1-0.5 parts of arginine, 1-10 parts of supramolecular peony peptide composition and the balance of water.

10. The method for preparing a cosmetic according to any one of claims 7 to 9, characterized in that: The following steps are involved: (1) mixing glycerin, butylene glycol, carbomer, xanthan gum, p-hydroxyacetophenone, sodium gluconate and water, and then heating and stirring to obtain a mixture A, and keeping the mixture A warm; (2) mixing olive oil emulsified wax, dicaprylyl carbonate, and behenyl alcohol and heating until the resulting solution turns yellow to obtain a mixture B; (3) Mixing mixture A and mixture B homogenously and cooling to obtain mixture C; (4) 1,2-hexanediol, arginine and the supramolecular peony peptide composition are sequentially added to the mixture C and mixed evenly, and sieved to obtain the cosmetic.

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