A marine algae composition having soothing and repairing efficacy

Abstract

The present application provides a marine alga composition with soothing and repairing effects, which combines sea fennel extract and d. capillata extract, inhibits skin inflammation, promotes expression of skin protective proteins, solves skin inflammation, and has excellent soothing and moisturizing and repairing effects, fundamentally solves a series of problems of the skin, has high and efficient effects, and has a good application prospect.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, and in particular to a marine algae composition with soothing and repairing properties. Background Technology

[0002] Sea fennel, a naturally salt-tolerant plant, mostly grows on sea rocks and sandy beaches along the Mediterranean, Black Sea, and Atlantic coasts of Europe, demonstrating remarkable resilience. Its unique aroma is widely loved by consumers, and it is used as an edible vegetable or spice in many European countries. Sea fennel is rich in bioactive compounds, such as vitamin C, omega-3 and omega-6 fatty acids (e.g., alpha-linolenic acid, linoleic acid), minerals, and trace elements, showing great potential and application value in pharmaceutical and cosmetic raw materials.

[0003] Kappa longifolia belongs to the red algae and contains a variety of active ingredients, such as carrageenan, fatty acids, amino acids, various mineral elements, β-carotene and red algal bile proteins. It has good effects in anti-oxidation and anti-aging, and has both medicinal and edible value. It is an important tropical marine biological resource.

[0004] When skin is stimulated by external factors, it becomes uncomfortable, and epidermal cells produce corresponding inflammatory factors and mediators. Therefore, inhibiting these inflammatory factors and mediators helps soothe the skin. At the same time, repairing and maintaining a healthy skin barrier helps the skin better resist external environmental factors, maintain internal homeostasis, and prevent skin moisture loss.

[0005] There is limited research on the target mechanisms of action of sea fennel extract, Kappa longicornis extract, and their combinations in existing technologies, especially regarding multi-target effects (soothing and repair). Furthermore, most patents include other active ingredients, failing to highlight their true efficacy. In addition, there is almost no research on the soothing and repairing effects of Kappa longicornis extract; current patent discussions on this ingredient mainly focus on anti-aging. Summary of the Invention:

[0006] To address the above problems, the present invention provides a composition comprising sea fennel extract and long-hearted kappa algae extract.

[0007] Furthermore, the concentration of the sea fennel extract is 0.00001%-1%, and the concentration of the long-hearted Kappa algae extract is 0.00001%-1%.

[0008] This invention provides the use of a composition for preparing a skin care formulation with soothing and repairing effects.

[0009] Furthermore, the composition includes sea fennel extract and long-hearted kappa algae extract.

[0010] Furthermore, the composition achieves its soothing and repairing purpose by inhibiting inflammatory targets and / or promoting barrier protein expression.

[0011] Furthermore, the inflammatory target is PGE2.

[0012] Studies have shown that the composition has a significant synergistic effect in inhibiting PGE2 expression.

[0013] Furthermore, the barrier protein is FLG and / or LOR.

[0014] Filagrin (FLG) is a basic protein component of keratinocytes (clear granules) in the basal cells of mammalian skin. It can specifically bind to intermediate filaments. Basal cells are undifferentiated juvenile cells (stem cells) with active proliferative capacity. As newly formed cells migrate to the superficial layers, they gradually differentiate to form the cells of the remaining layers of the epidermis; therefore, the basal layer is also called the germinal layer. Electron microscopy reveals numerous rough endoplasmic reticulum and tonofilaments within the cytoplasm.

[0015] Loricrin (LOR) is a major component of the keratinized capsule of ultimately differentiated keratinocytes and a substrate for transglutaminase. It exists in a dual cross-linked form consisting of disulfide bonds and N-(γ-glutamine)-lysine isopeptide bonds. Human loricrin consists of 316 amino acid residues.

[0016] Studies have shown that the composition has a significant synergistic effect in promoting FLG and / or LOR expression.

[0017] This invention provides the use of a composition for preparing an agent that inhibits the expression of inflammatory targets.

[0018] Furthermore, the composition includes sea fennel extract and long-hearted kappa algae extract.

[0019] Furthermore, the inflammatory target is PGE2.

[0020] This invention provides the use of a composition for preparing a formulation that promotes the expression of barrier proteins.

[0021] Furthermore, the composition includes sea fennel extract and long-hearted kappa algae extract.

[0022] Furthermore, the barrier protein is FLG and / or LOR.

[0023] Significant advantages of this invention:

[0024] 1. This invention demonstrates, using an SLS-3D epidermal model, the pathways and targets of action of sea fennel extract, long-hearted kappa algae extract, and their combinations in inhibiting skin inflammation and thus providing a soothing effect.

[0025] 2. This invention demonstrates, using an SLS-3D epidermal model, the pathways and targets of action of sea fennel extract, long-hearted kappa algae extract, and their combinations in enhancing skin barrier proteins and thus providing repair effects.

[0026] 3. This invention provides a combination of sea fennel extract and long-heart kappa algae extract, which has excellent soothing, moisturizing and repairing effects. Attached image description:

[0027] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0028] Figure 1 The effects of sea fennel extract, long-hearted Kappa algae extract, and the combination in Example 2 on the expression of inflammatory mediators.

[0029] Figure 2 The effects of sea fennel extract, long-hearted Kappa algae extract, and their combination on loricrin (LOR).

[0030] Figure 3 The effects of sea fennel extract, long-hearted Kappa algae extract, and their combination on filaggrin (FLG).

[0031] Specific Implementation Cases

[0032] The preferred embodiments of the present invention will be described in further detail below with reference to the accompanying drawings. It should be noted that the following embodiments are intended to facilitate the understanding of the present invention and are not intended to limit it in any way. All features disclosed in the embodiments of the present invention, or all steps in all methods or processes disclosed, except for mutually exclusive features and / or steps, can be combined in any way.

[0033] The sea fennel extract in the following examples, whose cosmetic INCI name is Crithamum maritimum extract, is an oil-soluble extract prepared from the sea fennel plant. This extract contains various active ingredients, such as vitamin C, omega-3 and omega-6 fatty acids (e.g., alpha-linolenic acid, linoleic acid), minerals, and trace elements. Suitable sea fennel extracts may include Samphira Oil Bioactive (purchased from CODIF).

[0034] The *Kappa sylvatella* extract in the following examples, whose cosmetic INCI name is *Kappa sylvatella* extract, is a water-soluble extract obtained from the *Kappa sylvatella* plant. This extract contains various active ingredients such as carrageenan, fatty acids, amino acids, various minerals, β-carotene, and phycobiliproteins. Suitable *Kappa sylvatella* extracts may include Kiribus BG (purchased from Katakura Coupa Guri Co., Ltd.). Example 1: A marine algae composition with soothing and repairing effects.

[0035] The composition described in this invention is obtained by mixing 0.00001%-1% of sea fennel extract with 0.00001%-1% of long-hearted Kappa algae extract. This composition is then used to conduct experiments on inhibiting inflammation and promoting protein expression. The content of the inflammatory mediator PGE2 is measured and recorded by ELISA, and the content of Loricrin (LOR) and Filaggrin (FLG) is measured and recorded by immunofluorescence.

[0036] Implementation Case 2: Inhibitory effects of sea fennel extract, long-hearted kappa algae extract, and their combination on the expression of inflammatory mediators.

[0037] Using the method described in Implementation Case 1, a 3D epidermal skin model stimulated by the anionic surfactant sodium lauryl sulfate (SLS) was selected. The SLS-3D epidermal model (hereinafter referred to as the SLS-3D epidermal model) was used to test the inhibitory effect of 0.03% sea fennel extract, 0.5% Kappa longifolia extract, and a combination thereof on the inflammatory mediator PGE2. The PGE2 content was determined using ELISA. The experimental results are shown in Table 1 and the accompanying instructions. Figure 1 As shown.

[0038] Table 1: Inhibitory effects of sea fennel extract, Kappa longifolia extract, and their combination on the expression of inflammatory mediators.

[0039] Sample group Composition Average concentration Sample 1 BC blank control 107.89 Sample 2 NC negative control 319.78 Sample 3 PC positive control 141.88 Sample 4 Crithmum maritimum extract 255.28 Sample 5 Kappaphycus alvarezii extract 310.03 Sample 6 Crithmum maritimum extract + Kappaphycus alvarezii extract 226.67

[0040] Data from Table 1 and the instruction manual Figure 1It was found that the extract of *Kappa longifolia* could not significantly inhibit the content of the inflammatory mediator PGE2, while the extract of *Flammulina salsa* and its combination could significantly inhibit the content of the inflammatory mediator PEG2. Moreover, the combination showed the best inhibition and was significantly better than the single-ingredient sample group. This indicates that the extract of *Flammulina salsa* has an inhibitory effect on the expression of PGE2, and the extract of *Kappa longifolia* can help the extract of *Flammulina salsa* to better inhibit the expression of PGE2. Therefore, the combination of the extract of *Flammulina salsa* and the extract of *Kappa longifolia* has a synergistic effect and has a more significant inhibitory effect on the expression of PGE2.

[0041] Within the range of 0.00001%-1% sea fennel extract and 0.00001%-1% long-heart kappa extract, we found that the inhibitory effect of the combination was significantly better than that of the single sample. Analysis of variance showed that the effect of the combination was significantly different from that of individual samples 4 or 5 (P < 0.05). Detailed analysis and data are omitted.

[0042] Case Study 3: The Promoting Effects of Sea Fennel Extract, Kappa longifolia Extract, and their Combinations on the Expression of Loricrin (LOR)

[0043] Using the method described in Implementation Case 1, an SLS-3D epidermal model was selected as the test model. 0.03% sea fennel extract, 0.5% *Kappa longifolia* extract, and a combination thereof were chosen. Immunofluorescence was used to test the content of loricrin (LOR). The experimental results are shown in Table 2 and the appendix to the specification. Figure 2 As shown.

[0044] Table 2: The promoting effects of sea fennel extract, long-hearted kappa algae extract, and their combination on the expression of loricrin (LOR).

[0045]

[0046]

[0047] The experimental data in Table 2 and the instruction manual are attached. Figure 2 It can be seen that sea fennel extract, Kappa longicornis extract, and the combination can all promote the content of loricrin (LOR). The combination has the best promoting effect and is significantly better than the single raw material sample group. This indicates that sea fennel extract, Kappa longicornis extract, and the combination have a promoting effect on the expression of loricrin (LOR), and the combination has a synergistic effect, with a more obvious promoting effect on the expression of loricrin (LOR).

[0048] Within the range of 0.00001%-1% sea fennel extract and 0.00001%-1% long-heart kappa algae extract, we found that the enhancement effect of the combination was significantly better than that of using either alone. Analysis of variance showed that the effect of the combination was significantly different from that of individual samples 4 or 5 (P < 0.05). Detailed analysis and data are omitted.

[0049] Implementation Case 4: The Promoting Effects of Sea Fennel Extract, Kappa longifolia Extract, and their Combinations on the Expression of Filagrin (FLG)

[0050] Using the method described in Implementation Case 1, an SLS-3D epidermal model was selected as the test model. 0.03% sea fennel extract, 0.5% *Kappa longifolia* extract, and a combination thereof were chosen. Immunofluorescence was used to test the content of filaggrin (FLG). The experimental results are shown in Table 3 and the appendix to the specification. Figure 3 As shown.

[0051] Table 3: The promoting effects of sea fennel extract, Kappa longifolia extract, and their combination on FLG protein expression.

[0052] Sample group Composition Relative IOD average Boost rate Sample 1 BC blank control 1 / Sample 2 NC negative control 0.44 / Sample 3 PC positive control 2.11 / Sample 4 Crithmum maritimum extract 0.67 52.27％ Sample 5 Kappaphycus alvarezii extract 0.56 27.27％ Sample 6 Crithmum maritimum extract + Kappaphycus alvarezii extract 0.84 90.90％

[0053] The experimental data in Table 3 and the instruction manual are attached. Figure 3 It can be seen that sea fennel extract, long-hearted Kappa algae extract, and the combination can all promote the content of filaggrin (FLG). The combination has the best promoting effect and is significantly better than the single raw material sample group. This indicates that sea fennel extract, long-hearted Kappa algae extract, and the combination have a promoting effect on the expression of filaggrin, and the combination has a synergistic effect, with a more obvious promoting effect on the expression of filaggrin.

[0054] Within the range of 0.00001%-1% sea fennel extract and 0.00001%-1% long-heart kappa algae extract, we found that the enhancement effect of the combination was significantly better than that of using either alone. Analysis of variance showed that the effect of the combination was significantly different from that of individual samples 4 or 5 (P < 0.05). Detailed analysis and data are omitted.

[0055] Implementation Case 5: A cosmetic formulation containing this combination of active ingredients

[0056] This composition can be formulated with different systems and can be used in various products such as toners, serums, lotions, creams, body oils, and shampoos. This example briefly describes a serum containing this composition; the relevant formula can be found in Table 4.

[0057] Table 4: A cosmetic formulation containing this combination of active ingredients

[0058] Ingredient Content (%) Water The rest is made up with this raw material Caprylic / capric triglyceride 1-10 Isododecane 1-10 Glycerin 1-10 Butylene glycol 1-10 Glyceryl stearate 1-10 Cetearyl alcohol 1-10 Butyrospermum parkii (shea) butter 1-10 Behenyl alcohol 1-10 Dimethicone 1-10 Hydrogenated lecithin 1-10 Glycosyl trehalose 0.1-1 Hydrogenated starch hydrolysate 0.1-1 p-Hydroxyacetophenone 0.1-1 Cetearyl glucoside 0.1-1 PEG- 100 stearate 0.1-1 1,2-Hexanediol 0.1-1 Simmondsia chinensis (jojoba) seed oil 0.1-1 Xanthan gum 0.1-1 Bisabalol 0.1-1 Squalane 0.1-1 Disodium EDTA 0.1-1 Potassium hydroxide 0.1-1 Kappaphycus alvarezii extract 0.00001-1 Crithmum maritimum extract 0.00001-1 Zostera marina extract Trace amount Hydrolyzed red algae extract Trace amount Seawater Trace amount 1,2-Pentanediol Trace amount Phenylethyl alcohol Trace amount Tocopherol (vitamin E) Trace amount

[0059] The above-described embodiments illustrate the technical solution of the present invention. Further research will be conducted on the process, formulation, and application. It should be understood that the above descriptions are only specific embodiments of the present invention and are not intended to limit the present invention. Any modifications, additions, or similar substitutions made within the scope of the principles of the present invention should be included within the protection scope of the present invention.

[0060] While the present invention has been disclosed above, it is not limited thereto. Any person skilled in the art can make various modifications and alterations without departing from the spirit and scope of the invention; therefore, the scope of protection of the present invention should be determined by the scope defined in the claims.

Claims

1. Use of a composition for the manufacture of a preparation for achieving a soothing, repairing efficacy, characterized in that, The composition is sea fennel extract and capa longifolia extract; the concentration of the sea fennel extract is 0.00001%-1%, and the concentration of the capa longifolia extract is 0.00001%-1%; the composition has a synergistic effect on inhibiting PGE2 expression; and the composition has a synergistic effect on promoting FLG and LOR expression.

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