Mounting device for installing sample separation unit

Abstract

The present invention relates to an installation device (100) for mounting a sample separation unit (30) for separating fluid samples in a sample separation apparatus (10). The installation device (100) includes: a first fluid port (102) for fluid connection to a first fluid connection point (104) of the sample separation unit (30); an actuation unit (110) operable by a user to move between an access position accessible by the user and a fluid coupling position of the fluidly coupled sample separation unit (30); and a fluid coupling mechanism (112) designed to form a force-loaded fluid coupling between the first fluid port (102) and the first fluid connection point (104) when the sample separation unit (30) is moved by the actuation unit (110) to the fluid coupling position.

Description

Technical Field

[0001] The present invention relates to an installation apparatus and method for installing a sample separation unit for separating fluid samples in a sample separation device, and to a sample separation device. Background Technology

[0002] In HPLC, a liquid (mobile phase) typically moves through a stationary phase (e.g., a column) at a very precisely controlled flow rate (e.g., in the range of microliters to milliliters per minute) and under high pressure (typically 20 to 1000 bar and more, currently up to 2000 bar) (where the compressibility of liquids is significant) to separate the individual components of the sample liquid introduced into the mobile phase from one another. Such an HPLC system is known, for example, from EP 0,309,596B1 of the same applicant, Agilent Technologies.

[0003] During the separation process, it may be necessary or desirable for the column to reach a desired temperature. For this purpose, the column is mounted in a column oven and heated there. Mounting the column in a column oven is typically inconvenient for the user. During such mounting, the user must handle small and sensitive components, such as capillaries and fittings, and couple them to the column. This constitutes a challenging activity that cannot be performed by a user without specialized knowledge.

[0004] GB 2564603, JP 07239325 and JP 03776446 disclose conventional systems for installing separation columns. Summary of the Invention

[0005] The object of this invention is to enable the sample separation unit to be installed quickly, error-proof, and conveniently by the user in a sample separation device, and to obtain accurate separation results after the sample separation unit is installed. This object is achieved by the independent claim. Other embodiments are shown in the dependent claims.

[0006] According to an exemplary embodiment of the present invention, an installation device is provided for installing a sample separation unit for separating fluid samples in a sample separation apparatus, wherein the installation device includes: a first fluid port for fluid connection to a first fluid connection point of the sample separation unit (optionally, a second fluid port for fluid connection to a second fluid connection point of the sample separation unit); an actuation unit capable of being actuated by a user to move between an access position where the user can approach the sample separation unit and a fluid coupling position where the sample separation unit is fluidly coupled; and a fluid coupling mechanism configured to form a force-loaded fluid coupling between the first fluid port and the first fluid connection point (and optionally between the second fluid port and the second fluid connection point) when the sample separation unit is moved by the actuation unit to the fluid coupling position.

[0007] According to another exemplary embodiment, a sample separation apparatus is provided for separating a fluid sample (e.g., in component form) from a mobile phase, wherein the sample separation apparatus includes: a fluid delivery unit for delivering a mobile phase and a fluid sample; a sample separation unit for separating the fluid sample (e.g., in component form) from the mobile phase delivered by the fluid delivery unit; and a mounting device having the above features, wherein the sample separation unit is fluidly mounted in the sample separation apparatus via the mounting device.

[0008] According to yet another exemplary embodiment, a method is provided for installing a sample separation unit for separating fluid samples in a sample separation apparatus, wherein the method includes: actuating an actuation unit of an installation device by a user to move the sample separation unit from an access position that allows the user to approach the sample separation unit to a fluid coupling position that fluidly couples the sample separation unit in the sample separation apparatus; and activating a fluid coupling mechanism by actuating only the actuation unit to move the sample separation unit to the fluid coupling position, thereby simultaneously forming a force-loaded fluid coupling of the sample separation unit in the sample separation apparatus.

[0009] In the context of this application, the term "sample separation apparatus" may specifically refer to a device capable of and configured to separate fluid samples (e.g., separate them into different components). For example, sample separation can be performed by chromatography or electrophoresis. For example, a sample separation apparatus may be a liquid chromatography sample separation apparatus.

[0010] In the context of this application, the term "fluid sample" specifically refers to a medium that includes fluids (particularly liquids and / or gases, optionally including solid particles) and contains a substance to be analyzed (e.g., biological samples, such as protein solutions, drug samples, etc.).

[0011] In the context of this application, the term "mobile phase" specifically refers to a fluid (particularly a liquid and / or gas, optionally including solid particles) used as a carrier medium for transporting a fluid sample from a fluid drive to a sample separation unit. For example, the mobile phase can be a solvent (e.g., organic and / or inorganic) or a solvent composition (e.g., water and ethanol).

[0012] In the context of this application, the term "sample separation unit" specifically refers to a component that can be inserted into a sample separation apparatus and, when the sample separation apparatus is in operation, causes the separation of a fluid sample into different components, either alone or in conjunction with other components. In particular, such a sample separation unit can be a chromatographic column or a component for electrophoretic separation. For example, a chromatographic column can include a container filled with a stationary phase, wherein the stationary phase can adsorb different components of the fluid sample as it flows between the inlet and outlet (i.e., between two fluid junctions). Furthermore, the stationary phase can be configured, particularly based on the mobile phase flowing through the sample separation unit in the form of a solvent composition, to desorb and / or release the adsorbed components in stages.

[0013] In the context of this application, the term "actuator" specifically refers to a device that can be manually operated by a user, and that can be moved to different positions by, for example, the user's mechanical actions. In particular, the actuator is configured such that when the user actuates the actuator, the actuator also causes the sample separation unit to receive the sample and to move the sample separation unit between different spatial positions / orientations within the mounting device.

[0014] In the context of this application, the term "fluid port" specifically refers to a component configured for fluid connection (particularly in a fluid-sealed manner, and further particularly in a high-pressure-sealed manner) to the inlet or outlet of a sample separation unit. In particular, such a fluid port may be configured as an accessory.

[0015] In this application, the term "fluid connection point" specifically refers to the inlet or outlet of a sample separation unit. Such a fluid connection point can be configured to fluidly connect to a fluid port of the mounting device.

[0016] In the context of this application, the term "access position" specifically refers to a defined operating position (distinct from a fluid coupling position) in which a user can access a receiving unit, such as a sample separation unit, to insert or remove the sample separation unit from the mounting device. In the access position, the sample separation unit received at the receiving unit may not be fluidly or even fluidly tightly coupled to the two fluid ports of the mounting device. Thus, in the access position, the user can insert or remove the sample separation unit from the mounting device without manually forming or releasing the fluid connection of the sample separation unit.

[0017] In the context of this application, the term "fluid-coupled position" specifically refers to a defined (and distinct from an access position) operating position in which a fluid coupling (or even a fluid seal and / or high-pressure seal) is formed between the sample separation unit and the fluid port of the mounting device. In the fluid-coupled position, for example by bringing the sample separation unit into the interior of the housing of the mounting device in the fluid-coupled position, it is possible to prevent the user from accessing the sample separation unit received in the mounting device.

[0018] In the context of this application, the term "fluid coupling mechanism" specifically refers to a mechanism (preferably purely mechanical) that can be configured from a configuration where the sample separation unit is fluidly decoupled from one or both fluid ports of the mounting device at the entry position, to a configuration where the sample separation unit is fluidly coupled to the fluid ports of the mounting device at the fluid coupling position, and vice versa. The actuation unit and the fluid coupling mechanism can cooperate such that actuating the actuation unit triggers activation or deactivation of the fluid coupling mechanism.

[0019] According to an exemplary embodiment, a mounting device is provided for user-friendly and reliable simultaneous installation and / or removal of a sample separation unit (e.g., configured as a chromatographic column). With this mounting device, after the user inserts the sample separation unit into the insertion position, actuation of the actuation unit alone is sufficient to move the sample separation unit from the insertion position to the operating position, where the sample separation unit is then ready for sample separation without further action. Here, the insertion position of the sample separation unit corresponds to the entry position, and the operating position of the fluid separation unit corresponds to the fluid coupling position. Advantageously, simply spatially moving the sample separation unit to the operating position can simultaneously result in fluid coupling between the sample separation unit and one or both fluid ports of the mounting device. Apart from inserting the sample separation unit and intuitively actuating the actuation unit (e.g., pushing a pull-out member into the housing of the mounting device), the user does not need to do anything else to achieve fluid coupling of the sample separation unit in the sample separation device. This allows even users without special expertise to reliably install the sample separation unit in the sample separation device while simultaneously forming fluid coupling, enabling the sample separation unit to operate for fluid separation via the fluid separation device. The handling of capillaries and the like is time-consuming, delicate, and error-prone. When using the mounting device according to an exemplary embodiment of the present invention, the connection between the capillaries and the sample separation unit is unnecessary. Therefore, the mounting device enables quick, error-proof, and user-friendly installation of the sample separation unit in the sample separation device. After the sample separation unit is installed, accurate separation results can be obtained because the sample separation units in the sample separation device reliably form fluid coupling.

[0020] Further embodiments of the installation apparatus, sample separation equipment, and method will be explained below.

[0021] According to an embodiment, the mounting device may include a second fluid port for fluid connection to a second fluid connection point of the sample separation unit, wherein the fluid coupling mechanism is configured to form a force-loaded fluid coupling between the first fluid port and the first fluid connection point, and between the second fluid port and the second fluid connection point, when the sample separation unit is moved to the fluid coupling position by the actuation unit. Therefore, two fluid connection positions of the column can be provided, which are coupled to two fluid ports when the mechanism is actuated.

[0022] Alternatively, a fluid connection can be automatically established using only one fluid port. For example, the column may already be fully fluidly coupled on one side, and a second fluid connection point of the column may also be fluidly coupled only when the actuation unit is activated (e.g., when the pull-out is moved to the closed position).

[0023] According to an embodiment, the actuation unit may include a pull-out element. The pull-out element is an actuation device that can be intuitively actuated by a user, moving only between two end positions in a mistake-proof manner to transfer the actuation unit between an inlet position and a fluid coupling position. Advantageously, the open state of the pull-out element may correspond to the inlet position, while the closed state of the pull-out element may correspond to the fluid coupling position.

[0024] According to an embodiment, the actuation unit can be configured to induce fluid coupling between a first fluid port and a first fluid connection point and / or between a second fluid port and a second fluid connection point through the movement of the pull-out member. In other words, by simply closing the pull-out member, it can act on the fluid coupling mechanism to achieve fluid coupling assisted by coupling force. In particular, the user can cause a fluid coupling position to be formed simply by closing the pull-out member.

[0025] According to an embodiment, the pull-out component can be configured to extend from the housing to adjust the entry position and retract into the housing to adjust the fluid coupling position. In the fluid coupling position, the pull-out component is preferably flush with the housing, and the user is not allowed to approach the operable sample separation unit. This improves operational safety because the user neither interferes with the ongoing separation process nor makes unintended contact with the sample separation unit, such as when it is heated during separation operation. Furthermore, the described actuation logic is highly intuitive for the user.

[0026] According to an embodiment, the pull-out element can be configured to increase the distance between the first and second fluid ports when moving from the fluid-coupled position to the inlet position, particularly when releasing a sample separation unit previously fluid-sealed between the first and second fluid ports. Conversely, when the actuation unit moves to its fluid-coupled position, the distance between the two fluid ports can be reduced to the length of the sample separation unit. The mechanical energy required to change the distance between the two fluid ports can be applied by the user when actuating the actuation unit to move between the inlet and fluid-coupled positions.

[0027] According to an embodiment, the mounting device may include at least one guide rail along which the pull-out member is guided. In this way, only longitudinal movement of the pull-out member is allowed between the two said end positions, thereby preventing erroneous operation.

[0028] According to an embodiment, the fluid coupling mechanism at the fluid coupling location can be configured to form a high-pressure seal (particularly a pressure of at least 100 bar, and more particularly at least 1000 bar) fluid coupling between the first fluid port and the first fluid connection point, and between the second fluid port and the second fluid connection point. Preferably, the fluid connection can withstand pressures of at least 1200 bar or even at least 1500 bar without leakage of the fluid sample or mobile phase between the respective fluid ports of the mounting device and the respective fluid connection points of the sample separation unit. For example, such pressures can be found in modern HPLC sample separation equipment.

[0029] According to an embodiment, the first fluid port may be a first fitting and / or the second fluid port may be a second fitting. Fluid ports configured as fittings can form a fluid connection between the sample separation unit and a fluid conduit or capillary adjacent to the corresponding fluid port, for example, through an inverted conical component, a collar, a housing component that can be threaded together, a sealing element, etc.

[0030] According to an embodiment, the first fluid port can be fixedly attached to the actuation unit. For example, the first fluid port can be rigidly mounted to a pull-out member that can form the actuation unit. When the sample separation unit is inserted into the mounting device, the user can place the first fluid connection point at the first fluid port, while in the inserted position, the second fluid connection point can be positioned at a certain distance from the second fluid port.

[0031] According to an embodiment, the second fluid port can be configured to be displaceable along the actuation unit. For example, in an embodiment, the second fluid port can be moved relative to a pull-out member that can form the actuation unit by user actuation. When the actuation unit is moved from the access position to the fluid coupling position, the displaceable or slidable second fluid port moves toward the fixed first fluid port. The two fluid ports then engage the sample separation unit on both sides in a fluid coupling force locking manner. In this way, the complex actions that the user must perform regarding the capillary and small connecting parts are unnecessary; instead, the fluid coupling is automatically performed by the mounting device. On the other hand, this fluid coupling is advantageously performed with the application of a defined coupling force, which is provided by the user during the defined movement of the actuation unit from the access position to the fluid coupling position, and this defined coupling force can be limited by a stop in the fluid coupling position. Advantageously, this avoids excessively low coupling forces, thereby ensuring a reliable fluid seal. However, at the same time, it is also impossible to generate excessive coupling forces that could damage the fluid ports and / or the sample separation unit. In other words, the coupling force is defined and pre-given by the construction of the mounting device and applied to the sample separation unit.

[0032] According to an embodiment, the actuation unit can be configured to change the position and / or orientation of the sample separation unit when shifting between an entry position and a fluid coupling position. Therefore, the processing position of the sample separation unit, which can be installed and / or removed by the user, can differ from the operating position of the sample separation unit fluidly coupled to the sample separation apparatus for sample separation. Thus, the shift between the entry position and the fluid coupling position is also accompanied by spatial displacement and / or reorientation of the sample separation unit. This advantageously allows the user to process the sample separation unit spatially (especially at ambient temperature) from external and / or from the often harsh conditions (e.g., high temperatures in a column furnace) at the operating position of the sample separation unit within the sample separation apparatus.

[0033] According to an embodiment, the fluid coupling mechanism can be configured to apply a pulling force to the second fluid port for force loading. Descriptively, the fluid coupling mechanism can pull the second fluid port in the direction of the first fluid port, thereby forming a fluid coupling between the fluid port and the sample separation unit disposed therebetween.

[0034] According to an embodiment, the fluid coupling mechanism can be configured to apply force via a tension element, particularly a tension rope, which is placed under tensile stress, especially a stretchable (German: spannbar), by transferring the sample separation unit to the fluid coupling position. The tension rope is a mechanical embodiment of the tension element, which operates in a simple and error-proof manner to induce force transmission to a movable fluid port and can also deflect this force, allowing the mounting device to be formed in a compact manner. Alternatively, however, force transmission can be performed by other mechanical mechanisms, such as by using gears.

[0035] According to an embodiment, a tension element configured as a tension cord can be fixedly attached to the actuation unit at one end and coupled to a second fluid port at the other end. Descriptively, when the actuation unit (specifically configured as a pull-out element) is moved from an access position to a fluid coupling position, the fixed end of the tension cord can follow the movement of the pull-out element, thereby applying a tension force along the tension cord to the movable fluid port, causing the movable fluid port to move toward the fixed fluid port. In this way, through an error-proof mechanism, the user's actuation force can be converted into a coupling force between the sample separation units of the fluid ports.

[0036] According to embodiments, the mounting device may include a tensioning unit, particularly a spring unit, for generating a tensioning force that promotes fluid coupling upon transfer to the fluid coupling position. For example, the mounting device may include a spring unit at and / or within a tension cord. This tensioning unit can generate a biasing force by which the sample separation unit in the fluid coupling position is fluid-tightly clamped between the two fluid ports of the mounting device. Advantageously, this tensioning unit may be configured as a mechanical spring (particularly in a tension cord) that is tensioned upon transfer to the fluid coupling position to generate the biasing force. For example, this mechanical spring may be a helical spring or a leaf spring, or it may be multiple individual mechanical springs (e.g., a leaf spring encapsulation). The biasing force of the tensioning unit can also be generated in another manner, for example, by magnetic elements that attract each other, which are separated from each other upon transfer to the fluid coupling position, thereby generating a restoring magnetic force as the biasing force.

[0037] According to an embodiment, the mounting device may include at least one deflection element, particularly at least one deflection roller, around which a tension element, particularly a tension rope, deflects. By providing one or more deflection rollers or other deflection elements, the mounting device can be implemented even in confined space conditions, because the tension rope deflected by the deflection roller can be arranged in almost any free space within the mounting device.

[0038] According to an embodiment, the mounting device may include a receiving unit, particularly a column bed, configured to align and receive a sample separation unit for fluid coupling between a first fluid port and a second fluid port. For example, a user may insert the sample separation unit into the receiving unit (e.g., horizontally), or place or insert it onto the receiving unit (e.g., vertically). The sample separation unit automatically positions and / or is correctly oriented at the receiving unit so that it can be transferred to the fluid coupling position solely by actuation of the actuating unit.

[0039] According to an embodiment, the mounting device can be configured to install and / or remove the sample separation unit without the need for user tools. This allows for user operation of the mounting device in a particularly simple manner, eliminating the need for complex activities such as screwing capillary tubes to fittings by the user.

[0040] In an embodiment, the mounting device may include a preheating unit (particularly directly upstream of the first fluid port) for preheating the fluid sample before it reaches the sample separation unit. Such a preheating unit can bring the fluid sample to an increased target temperature directly before separation in the sample separation unit, thereby creating defined or beneficial separation conditions. When such a preheating unit is arranged directly adjacent to the first fluid port, undesirable cooling effects between preheating and separation can be prevented or at least strongly suppressed.

[0041] According to an embodiment, the mounting device can be configured as a liquid chromatography column furnace. During separation, it may be necessary or desirable for the sample separation unit configured as a column to reach a desired temperature. For this purpose, the column is mounted in the column furnace and heated therein. Using the mounting device according to an exemplary embodiment of the invention, the user can mount the column in the column furnace in a comfortable manner because the sample separation unit is inserted outside the column furnace in the actuation unit entry position and is inserted into the column furnace only by transferring the actuation unit to the fluid coupling position. Therefore, the user can also handle the sample separation unit at ambient temperature rather than at the high temperatures that may exist in the column furnace (e.g., up to 100°C or even up to 150°C). This not only increases operational comfort but also increases operational safety.

[0042] According to an embodiment, the actuation unit and the fluid coupling mechanism can be configured to move in mutually parallel directions (compare) Figure 2 and Figure 3On the one hand, the actuation unit moves when shifting between the entry position and the fluid coupling position; on the other hand, relative movement occurs between the first fluid port and the second fluid port. In other words, the movement of the actuation unit, configured as a pull-out member, between the entry position and the fluid coupling position can occur in the same direction as the movement of the second fluid port toward the first fluid port (to form fluid coupling of the sample separation unit). This achieves a particularly simple mechanism. For example, the sample separation unit can be horizontally inserted into the receiving unit of the mounting device and can be fluidly connected (especially with a high-pressure seal) simply by closing the pull-out member.

[0043] According to another embodiment, the actuation unit and the fluid coupling mechanism can be configured to move in mutually orthogonal directions of motion (see, for example, see below). Figure 4 On the one hand, the actuation unit moves during the transition between the entry position and the fluid coupling position; on the other hand, relative movement occurs between the first fluid port and the second fluid port. For example, it is preferable to insert the sample separation unit (e.g., a chromatographic column) into the mounting device in a vertical orientation, and then trigger the fluid coupling of the fluid separation unit with a vertical coupling force through the horizontal displacement movement of the actuation unit configured as a pull-out element. Here, the reorientation of the force direction of the fluid coupling mechanism can be performed, for example, using a tension rope combined with a deflection roller.

[0044] According to an embodiment, the first fluid port and the second fluid port can be configured to fluidly connect to a horizontally oriented sample separation unit. Alternatively, the first fluid port and the second fluid port can be configured to fluidly connect to a vertically oriented sample separation unit. When moving from the entry position to the fluid coupling position, or vice versa, the sample separation unit can remain horizontal or preferably vertically oriented, or the sample separation unit can be reoriented (e.g., using a pivoting mechanism).

[0045] According to an embodiment, the actuation unit and / or fluid coupling mechanism can be configured to output feedback, particularly tactile feedback, indicating this condition when the transfer to the fluid coupling position is complete. For example, the sample separation unit can be audibly or perceptibly latched when a sealed fluid connection is formed between the sample separation unit and the two fluid ports of the mounting device. The user receives explicit feedback that the mounting process has been successfully completed, and sample separation can now be performed in a fluid-sealed manner.

[0046] According to embodiments, the sample separation device can be configured as, for example, a microfluidic measurement device, a liquid chromatography device, or an HPLC device. Therefore, the sample separation device can particularly be configured as an HPLC device (high-performance liquid chromatography), a life science device, or an SFC device (supercritical fluid chromatography). However, many other applications are also possible.

[0047] According to an embodiment, the sample separation device can be configured in a pressure-sealed manner for operation at pressures up to about 100 bar, particularly for operation at pressures up to about 500 bar, and even more particularly for operation at pressures up to about 1000 bar.

[0048] According to embodiments, the sample separation device may include a separation column as a sample separation unit for separating different components of an injected fluid sample. This separation column may be filled with an adsorption medium, such as porous beads made of silica gel or activated carbon. Through chemical interaction with these porous beads, the fluid sample can be temporarily immobilized or absorbed at the separation column. For example, by adjusting the gradient of the solvent composition, individual components of the fluid sample can be individually released or desorbed and subsequently detected.

[0049] According to an embodiment, the analytical pump can be configured to typically deliver the injected fluid sample via a mobile phase. The mobile phase can be a solvent composition that can be constant over time or can be adjusted in a manner and is mixed with the fluid sample after it has been inserted into the sample separation path via an injection valve. The mixture of the mobile phase and the fluid sample can be pumped through the chromatographic separation path by the analytical pump, which is configured as a high-pressure pump. Therefore, the sample separation apparatus can include one or more pumps for delivering the injected fluid sample along with the mobile phase through at least a portion of the sample separation apparatus. For example, such a pump can be configured to pump the mobile phase through the system at high pressures, such as from approximately 100 bar to up to 1000 bar and higher.

[0050] According to an embodiment, the sample separation device may include a sample detector for detecting sample components separated in a fluid sample. This sample detector may be based on a detection principle that detects electromagnetic radiation (e.g., in the UV range or the visible range) originating from certain sample components of the fluid sample.

[0051] Alternatively or additionally, the measuring device may include a sample fractionator for separating sample components. For example, such a fractionator may direct different sample components into different liquid containers. However, the analyzed fluid sample may also be supplied to a waste container. Attached Figure Description

[0052] Other objects and numerous accompanying advantages of the embodiments of the present invention will be readily appreciated and better understood by referring to the following detailed description of the embodiments in conjunction with the accompanying drawings. The same reference numerals will be used for features that are substantially or functionally identical or similar.

[0053] Figure 1 An HPLC sample separation apparatus according to an exemplary embodiment of the present invention is shown.

[0054] Figure 2 An installation device according to an exemplary embodiment of the present invention is shown in an operable state, wherein a user can insert a sample separation unit into the installation device or remove the sample separation unit from the installation device.

[0055] Figure 3 Showing Figure 2 The installation device shown is in another operable state, in which the user has inserted the sample separation unit into the installation device and, by means of an actuated pull-out element, has transferred the sample separation unit into the installation device under the force applied to form a fluid connection between the sample separation unit and the installation device.

[0056] Figure 4 An installation device according to another embodiment of the present invention is shown in an operable state, wherein a user can insert a sample separation unit into the installation device or remove the sample separation unit from the installation device.

[0057] The illustrations in the attached diagram are schematic. Detailed Implementation

[0058] Before describing exemplary embodiments of the invention with reference to the accompanying drawings, some considerations on which exemplary embodiments of the invention were developed will be explained.

[0059] According to an exemplary embodiment of the present invention, an installation device for installing a sample separation unit (e.g., a chromatographic separation column) is provided, wherein the user inserts the sample separation unit only into the entry position of the actuation unit, and by simply actuating the actuation unit, spatial transfer of the sample separation unit to a target position inside the sample separation device and automatic formation of fluid coupling between the inserted sample separation unit and the fluid port of the installation device can be achieved.

[0060] Specifically, for this purpose, a pull-out system can be implemented, which serves both to receive the sample separation unit and to move the sample separation unit to a target position, as well as to simultaneously form fluid coupling between the sample separation unit and the fluid ports of the sample separation device. By pulling out the pull-out, the fluid ports can be moved forward, thereby allowing the user to access them. Simultaneously, pulling out increases the distance between the fluid ports, facilitating the insertion of the sample separation unit. After insertion, the user only needs to move the pull-out backward, whereby the fluid coupling mechanism of the mounting device brings the fluid ports closer together until they engage at the fluid connection points of the sample separation unit on both sides, thus achieving fluid coupling of the sample separation unit. The kinematics of the mounting device can be configured so that the user does not need to do anything else to adjust the sample separation unit for sample separation via the sample separation device except to insert the sample separation unit and pull out or push in the pull-out. Advantageously, in such a mounting device, the position where the user inserts the sample separation unit externally and the position where the sample separation unit is used internally within the sample separation device (e.g., in the heating zone of a column furnace) are different from each other. This allows users to insert the sample separation unit outside of harsh conditions (such as high temperatures) that may be prevalent at the point of use during sample separation.

[0061] One of the two fluid ports of the mounting device can be connected to a preheating unit for preheating the sample to be separated and / or a mobile phase for that purpose, to operate the sample separation unit and the fluid flowing through it at a desired temperature. In different embodiments of the mounting device, insertion of the sample separation unit can be performed by horizontal or vertical insertion. When transferring the sample separation unit between the entry position and the fluid coupling position, the sample separation unit can be linearly displaced or pivoted (e.g., via a pivot rod and / or pivoting mechanism).

[0062] According to exemplary embodiments of the invention, the cooperation of the actuation unit and the fluid coupling mechanism of the mounting device can advantageously include transferring the sample separation unit from the insertion position to the operating position and forming a fluid coupling between the sample separation unit and the sample separation device, the fluid coupling accompanying the transfer without further action. According to a preferred embodiment, the fluid coupling can be a high-pressure seal, particularly a fluid seal at pressures up to at least 100 bar or preferably up to at least 1500 bar. Therefore, exemplary embodiments of the invention connect the sample separation unit to the sample separation device in a quick and safe manner without requiring special qualifications or skills from the user. Preferably, the actuation unit and / or the fluid coupling mechanism can be configured such that the user receives feedback when the fluid connection of the sample separation unit is correctly completed. For example, such feedback can be tactile, acoustic, and / or optical feedback. For example, locking the sample separation unit at the target position when a fluid connection is formed between the sample separation unit and the sample separation device during fluid coupling can be audible and / or noticeable, or a separate signal can be output.

[0063] For example, for chromatographic sample separation, it may be necessary or desirable to heat the sample separation unit to high temperatures, such as up to 100°C or even up to 150°C. Sample separation can also be performed at lower temperatures, up to 4°C. In this case, it is preferable that the fluid coupling mechanism is isolated from, for example, temperatures above or below ambient temperature and maintained at ambient temperature. This, for example, avoids the clamping force of the fluid coupling mechanism's spring mechanism from deviating from the target force due to thermal expansion.

[0064] According to an exemplary embodiment, the actuation unit configured as a pull-out member can be combined with a fluid coupling mechanism that performs fluid coupling of the sample separation unit triggered by the movement of the pull-out member. This enables tool-less column connection. In other words, column connection can be performed using an installation device that operates based on the pull-out member principle. This tool-less column connector is advantageous for simple, tool-free column installation and for high operational safety in forming a reliable fluid connection. The advantage of this embodiment is that the user does not need to use any tools and can still ensure that the installed sample separation unit is installed in a fluid-tight manner.

[0065] Therefore, according to embodiments of the invention, a mounting device for a liquid chromatography housing including a column compartment can be provided. Furthermore, a pull-out member can be provided, sliding on a track and attached to a spring-loaded displacement system. A column bed is provided in the pull-out member to ensure proper column alignment. The pull-out member may include a fixed fitting at the outermost end and a movable fitting at the proximal end. When the pull-out member is closed, a spring-loaded connection mechanism can be activated. Closing the pull-out member advantageously results in tensile stress at the column for a fluidly tight connection with the two fittings.

[0066] Figure 1 The main structure of an HPLC system is shown, as an example of a sample separation device 10, which can be used, for example, in liquid chromatography. A fluid delivery unit 20, configured as an analytical pump, drives the mobile phase through a separation device (e.g., a chromatographic column) serving as a sample separation unit 30. This mobile phase is supplied by at least one solvent container 25 and can be degassed by a degasser 27. The sample separation unit contains a stationary phase. A sample insertion unit, also referred to as a sample injector or simply an injector 40, is arranged between the fluid delivery unit 20 and the sample separation unit 30 to introduce a fluid sample into the mobile phase via a fluid switch (which can be considered part of the injector 40). The stationary phase of the sample separation unit 30 is used, for example, to separate the sample components of the sample liquid according to the principles of liquid chromatography. A detector 50 detects the separated sample components of the fluid sample, and a fractionation device 60 is provided to output the separated sample components of the sample liquid (e.g., in a container or discharge device provided for this purpose). A control unit 70 controls the components of the sample separation device 10.

[0067] When the fluid path between the fluid delivery unit 20 and the sample separation unit 30 is normally under high pressure, the sample liquid is first introduced at normal pressure into the area separated from the liquid path, namely the so-called sample loop (as a sample receiving space) of the sample injector 40, which then introduces the sample liquid into the high-pressure fluid path. When the sample liquid, which is initially in the sample loop at normal pressure, is introduced into the high-pressure liquid path, the contents of the sample loop suddenly (typically within milliseconds) reach the system pressure of the sample separation device 10.

[0068] from Figure 1 It can also be seen that the sample separation unit 30 is arranged in a temperature control chamber 92, which typically has a preheating unit 90, and can be heated there by a heating source 80. In this way, the sample separation unit 30 can be made to reach the desired or pre-given target temperature of the sample separation method.

[0069] also, Figure 1An installation apparatus 100 according to an exemplary embodiment of the present invention is schematically shown for installing and removing a sample separation unit 30 in a sample separation apparatus 10. (Refer to...) Figures 2 to 4 The preferred embodiment of this mounting device 100 is described below.

[0070] Figure 2 and Figure 3 An installation apparatus 100 according to an exemplary embodiment of the present invention is shown in different operating states for installing a sample separation unit 30 for separating fluid samples in a sample separation device 10, wherein the sample separation device 10 is configured herein to perform liquid chromatography. More specifically... Figure 2 An installation device 100 in an operational state is shown, wherein a user can insert a sample separation unit 30 configured as a chromatographic separation column into the installation device 100, or remove the sample separation unit 30 from the installation device 100 (e.g., for column replacement). Conversely, Figure 3 The mounting device 100 is shown in another operational state, wherein the user has inserted the sample separation unit 30 into the mounting device 100, and the sample separation unit 30 has been transferred into the interior of the mounting device 100 under the force of forming a fluidly coupled sample separation unit 30 by means of an actuated pull member 114. Figure 2 and Figure 3 The mounting device 100 is used for processing sample separation unit 30 at and within the column furnace (with) Figure 1 (Compared to reference numeral 92 in the attached figure).

[0071] Figure 2 and Figure 3 The mounting device 100 shown includes a first fluid port 102 for fluid connection to a first fluid connection point 104 of the sample separation unit 30. Here, the first fluid port 102 may be a high-pressure fitting that is fluidly connected to the first fluid connection point 104 of the sample separation unit 30, which is configured as a column inlet. The first fluid port 102 is rigidly attached to the pull-out member 114, and therefore moves uniformly together with the pull-out member 114 when moved by the user. Thus, the first fluid port 102 is fixedly attached to the actuation unit 110.

[0072] The mounting device 100 also includes a second fluid port 106 for fluid connection to a second fluid connection point 108 of the sample separation unit 30, the second fluid connection point 108 being configured as a column outlet. During operation of the sample separation device 10, a fluid sample or mobile phase from the injector 40 flows through the first fluid port 102 in the first fluid connection point 104 and through the sample separation unit 30, exiting through the second fluid port 106 in the second fluid connection point 108 of the sample separation unit 30, and subsequently... Figure 1 The fluid flows in the direction of the detector 50. The second fluid port 106 can be another high-pressure fitting. Both fluid ports 102 and 106 and the sample separation unit 30 can be configured to cooperate with the actuation unit 110 and the fluid coupling mechanism 112, which is described in detail below, to form a high-pressure sealed fluid connection that reliably prevents liquid leakage even at pressures of 1200 bar and higher. Compared to the first fluid port 102, the second fluid port 106 is configured to be displaceable along the actuation unit 110. In other words, relative movement occurs between the second fluid port 106 and the pull-out member 114 when the pull-out member 114 extends out of or retracts into the housing 116.

[0073] The mounting device 100 also includes the previously mentioned actuation unit 110, which can be actuated by the user to bring the sample separation unit 30 into an access position accessible to the user (compare). Figure 2 The fluid coupling position of the fluidly coupled sample separation unit 30 (see...) Figure 3 Transfer installation device 100 between ( ). For example, Figure 2 and Figure 3 As shown in the comparison, the actuation unit 110 is configured to, when shifting between the entry position and the fluid coupling position, according to... Figure 2 The insertion or removal position is based on Figure 3 The position of the sample separation unit 30 is changed between the fluid coupling position and the fluid separation position. Based on... Figure 2 In the access position, the user can insert the sample separation unit 30 into the receiving unit 126 configured as a column bed, or remove the sample separation unit 30 from the receiving unit 126 (e.g., for column replacement). Therefore, the receiving unit 126 configured as a column bed is used for aligning and receiving the sample separation unit 30 to achieve fluid coupling between the first fluid port 102 and the second fluid port 106. Thus, in the access position, the sample separation unit 30 is accessible to the user but not fluidly connected between the fluid ports 102, 106. According to... Figure 3In the fluid coupling position, the receiving unit 126 and the sample separation unit 30 are housed inside a user-inaccessible housing 116, preventing the user from accessing them. However, in contrast to the access position, the sample separation unit 30 in the fluid coupling position is fluidly connected within the sample separation device 10 by forming a first fluid seal connection between the first fluid port 102 and the first fluid connection point 104 and a second fluid connection between the second fluid port 106 and the second fluid connection point 108.

[0074] In order to achieve such fluid coupling of the sample separation unit 30 when the mounting device 100 transitions to the fluid coupling position, the mounting device 100 is equipped with a fluid coupling mechanism 112, which is configured to form a force-loaded fluid coupling between the first fluid port 102 and the first fluid connection point 104 and between the second fluid port 106 and the second fluid connection point 108 when the sample separation unit 30 is transferred to the fluid coupling position by the actuation unit 110.

[0075] The design of the actuation unit 110 and the fluid coupling mechanism 112 will be described in more detail below.

[0076] As described above, the actuation unit 110 includes a pull-out member 114 capable of horizontal movement. The corresponding direction of movement is... Figure 2 As shown by arrow 170. The actuation unit 110 is configured to form fluid coupling between the first fluid port 102 and the first fluid connection point 104 and between the second fluid port 106 and the second fluid connection point 118 via the movement of the pull member 114 along the displacement direction according to arrow 170. Furthermore, the pull member 114 is configured to, for the movement of the pull member 114 along the displacement direction according to arrow 170, form fluid coupling between the first fluid port 102 and the first fluid connection point 104 and between the second fluid port 106 and the second fluid connection point 118. Figure 2 The entry position extends from the housing 116, and for according to Figure 3 The fluid coupling position is retracted in the housing 116. Apart from inserting and / or removing the sample separation unit 30 and actuating the pull-out member 114, the user does not need to do anything further to enable the sample separation unit 30 to operate in the sample separation device 10 to perform sample separation tasks.

[0077] More specifically, the pull-out member 114 is configured to increase the distance between the first fluid port 102 and the second fluid port 106 when transferring from the fluid coupling position to the entry position, from according to Figure 3 The smaller distance value d2 begins to increase according to Figure 2 The larger distance value d1 > d2. As according to Figure 3The increased distance between the two fluid ports 102, 106 of the sample separation unit 30, which are engaged by force locking, releases the sample separation unit 30, which was previously fluid-tightly mounted between the first fluid port 102 and the second fluid port 106. This allows the sample separation unit 30 to be removed after the pull-out member 114 is pulled out of the housing 116. The increased distance between the fluid ports 102, 106 during the transition to the entry position also facilitates the insertion of a new sample separation unit 30 into the receiving unit 126.

[0078] Figure 2 and Figure 3 The mounting device 100 is shown to include a horizontally arranged guide rail 118 along which a pull-out member 114 is guided. Therefore, user error with the mounting device 100 is virtually eliminated, as the user must simply move the pull-out member 114 between two opposing end positions to install or remove the inserted sample separation unit 30.

[0079] pass Figure 2 and Figure 3 As can be seen, the fluid coupling mechanism 112 is configured to apply a sealing pull force to the second fluid port 106 for applying force loading to the sample separation unit 30 in the fluid coupling state. In the embodiment of the fluid coupling mechanism 112 shown, this is achieved by force loading caused by a tension cord 120. The tension cord 120 is tensioned by moving the sample separation unit 30 from the access position to the fluid coupling position. For this purpose, the tension cord 120 is fixedly attached at one end 130 to the actuation unit 110 configured as a pull-out member 114. Therefore, the end 130 moves uniformly together with the pull-out member 114. Conversely, at its opposite end 132, the tension cord 120 is coupled to the second fluid port 106. When the user closes the pull-out member 114 by movement in the direction of arrow 170, the first fluid port 102, the sample separation unit 30, and the end 130 are displaced in the housing 116 in a rigidly coupled manner and follow the movement of the pull-out member 114. Figure 2 The end 130 thus moves to the left, generating tension on the tension rope 120 at the end 130. This tension pulls the opposite end 132 of the tension rope 120 and the second fluid port 106 connected thereto toward the left. Figure 2 Pulling to the left. The corresponding direction of movement is indicated by arrow 172 and can be transmitted between end 132 and second fluid port 106 via optional force transmitter 174. Due to the described kinematics, second fluid port 106 approaches and ultimately engages second fluid connection point 108 with force lock. Thus, high-pressure fluid coupling is established between sample separation unit 30 and fluid ports 102, 106.

[0080] The spring unit 134 (or more generally, a biasing unit) in the tension rope 120 (constructed as a helical spring in the illustrated embodiment) is offset (stretched in more detail) during the process, thereby generating a restoring tension force at the end 132. This tension force tends to bias the second fluid port 106 against the second fluid connection point 108 of the sample separation unit 30. As a result, the close fluid coupling of the sample separation unit 30 is strengthened on both sides.

[0081] also, Figure 2 and Figure 3 Multiple deflection rollers 124 are shown, around which tension ropes 120 deflect. The deflection rollers 124 cooperate with the tension ropes 120 to serve as force converters, and the force-loaded fluid coupling described in the sample separation unit 30 can be achieved even under space-constrained conditions, and thus the force-loaded fluid coupling described is achieved compactly.

[0082] Advantageous, referencing Figure 2 and Figure 3 The operating mechanism of the described mounting device 100 allows the user to install and remove the sample separation unit 30 without tools. Therefore, special user expertise is unnecessary. Advantageously, the user is no longer required to handle miniaturized capillaries and fittings carefully.

[0083] By including a preheating unit 90 directly upstream of the first fluid port 102 in the mounting device 100, it is possible to preheat the fluid sample directly before it reaches the sample separation unit 30. Therefore, the simple-to-operate mounting device 100 can also achieve precise sample separation under defined predetermined separation conditions.

[0084] Parallel arrows 170 and 172 indicate that, according to Figure 2 and Figure 3 The actuation unit 110 and the fluid coupling mechanism 112 are configured to move in a horizontal direction parallel to each other in the illustrated embodiment, shifting between an entry position and a fluid coupling position on the one hand, and moving relative to each other between a first fluid port 102 and a second fluid port 106 on the other hand. Accordingly, according to Figure 2 and Figure 3 The first fluid port 102 and the second fluid port 106 are configured to fluidly connect to the horizontally oriented sample separation unit 30.

[0085] Advantageously, the actuation unit 110 and the fluid coupling mechanism 112 can be configured to output tactile feedback when the transfer to the fluid coupling position is completed. For example, a noticeable and audible latching can occur when the sample separation unit 30 is fluidly coupled to the fluid ports 102, 106.

[0086] Advantageously, according to Figure 2 and Figure 3 The configuration is compatible with the use of sample separation units 30 of different lengths. Sample separation units 30, which can be freely selected within a wider length range, are preferably placed on the receiving unit 126 in a left-aligned manner (i.e., adjacent to the first fluid port 102). Coupling of the second fluid port 106 is achieved by closing the pull-out member 114 according to... Figure 2 and Figure 3 The movement of the second fluid port 106 toward the left is performed until the second fluid port 106 abuts against the second fluid connection point 108 of the sample separation unit 30. When the pull-out member 114 is pushed in the housing 116, the fluid connection becomes tight. Therefore, the user can freely use sample separation units 30 of different sizes using the same mounting device 100.

[0087] Figure 4 An installation device 100 according to another exemplary embodiment of the present invention is shown, which is in an operable state in which a user can insert the sample separation unit 30 into the installation device 100 or remove the sample separation unit 30 from the installation device 100.

[0088] according to Figure 4 Implementation examples and according to Figure 2 and Figure 3 The main difference in the embodiments is that, according to Figure 4 The sample separation unit 30 is inserted vertically (instead of horizontally) into the mounting device 100, and the movement of the pull-out member 114 is performed according to the direction of arrow 170, which is perpendicular (and not parallel) to the movement of the second fluid port 106 toward the first fluid port 102 (see arrow 176).

[0089] Therefore, according to Figure 4 The actuation unit 110 and the fluid coupling mechanism 112 are configured such that, on one hand, the mounting device 100 is installed in a direction of motion that is orthogonal to each other (see reference numerals 170, 176). Figure 4 The transfer between the entry position shown and the fluid coupling position (not shown) is carried out, while relative movement between the first fluid port 102 and the second fluid port 106 is performed. Furthermore, the first fluid port 102 and the second fluid port 106 are configured for fluid connection to the vertically oriented sample separation unit 30.

[0090] exist Figure 4 In the middle, the first deflection roller 124a is attached to the pull-out member 114, while the second deflection roller 124b is rigidly mounted on the housing 116.

[0091] When the pull-out component 114 is according to Figure 4As the entry position begins to move within the housing 116 in the direction of movement indicated by arrow 170, the tension cord 120 is rigidly attached to the end 130 of the pull-out member 114. Figure 4 Move to the left, thereby applying tension to the tension cord 120. This tension is transmitted along the tension cord 120 to the opposite end 132, whereby the second fluid port 106 moves downward in the direction of arrow 176 and engages in a sealing manner with the second fluid connection point 108 of the sample separation unit 30.

[0092] It should be noted that the term "comprising" does not exclude other elements, and "a" does not exclude multiple elements. Elements described in conjunction with different embodiments can also be combined. It should also be noted that the reference numerals in the claims should not be construed as limiting the scope of the claims.

Claims

1. Mounting device (100) for mounting a sample separation unit (30) for separating a fluid sample in a sample separation apparatus (10), wherein The mounting device (100) includes: The first fluid port (102) is used for fluid connection to the first fluid connection point (104) of the sample separation unit (30). Actuation unit (110), which can be actuated by a user to move between an entry position that allows the user to approach the sample separation unit (30) and a fluid coupling position that fluidly couples the sample separation unit (30); and A fluid coupling mechanism (112) is configured to form a force-loaded fluid coupling between the first fluid port (102) and the first fluid connection point (104) when the sample separation unit (30) is transferred from the actuation unit (110) to the fluid coupling position. The actuation unit (110) includes a pull-out member (114) and is configured to induce fluid coupling between the first fluid port (102) and the first fluid connection point (104) by movement of the pull-out member (114).

2. The installation device (100) according to claim 1. Includes a second fluid port (106), which is used for fluid connection to the second fluid connection point (108) of the sample separation unit (30); wherein The fluid coupling mechanism (112) is configured to form a force-loaded fluid coupling between the first fluid port (102) and the first fluid connection point (104) and between the second fluid port (106) and the second fluid connection point (108) when the sample separation unit (30) is transferred from the actuation unit (110) to the fluid coupling position.

3. The mounting device (100) according to claim 2, wherein The actuation unit (110) is configured to induce fluid coupling between the second fluid port (106) and the second fluid connection point (108) by the movement of the pull member (114).

4. The mounting device (100) according to claim 3, wherein The pull-out member (114) is configured to extend from the housing (116) to the entry position and retract into the housing (116) to the fluid coupling position.

5. The mounting device (100) according to claim 3 or 4, wherein The pull-out member (114) is configured to increase the distance (d1, d2) between the first fluid port (102) and the second fluid port (106) when the sample separation unit (30) installed between the first fluid port (102) and the second fluid port (106) is released when the sample separation unit (30) is moved from the fluid coupling position to the entry position.

6. The mounting device (100) according to claim 2, wherein The first fluid port (102) is a first fitting and / or the second fluid port (106) is a second fitting.

7. The mounting device (100) according to claim 2, comprising at least one of the following features: wherein The first fluid port (102) is fixed to the actuation unit (110). The second fluid port (106) is configured to be able to move along the actuation unit (110) by moving the actuation unit (110).

8. The mounting device (100) according to claim 1, wherein The actuation unit (110) is configured to change the position and / or orientation of the sample separation unit (30) when it moves between the entry position and the fluid coupling position.

9. The mounting device (100) according to claim 2, wherein The fluid coupling mechanism (112) is configured to apply a pulling force to the second fluid port (106) for force loading.

10. The mounting device (100) according to claim 2, wherein The fluid coupling mechanism (112) is configured to apply the force by means of a tension element that can be transferred to the fluid coupling position by the sample separation unit (30) and is subjected to tensile stress.

11. The installation device (100) according to claim 10, wherein the tension element is a tension rope (120).

12. The mounting device (100) according to claim 10, wherein the tension element is stretchable.

13. The mounting device (100) according to any one of claims 10-12, comprising at least one of the following features: wherein, The tension element is fixed to the actuation unit (110) at one end (130) and coupled to the second fluid port (106) at the other end (132); It includes at least one deflection element, and the tension element deflects around the deflection element.

14. The mounting device (100) according to claim 13, wherein the at least one deflecting element is at least one deflecting roller (124).

15. The mounting device (100) according to claim 1, comprising a tensioning unit for generating a tensioning force that promotes fluid coupling when moved to the fluid coupling position.

16. The mounting device (100) according to claim 15, wherein the tensioning unit is a spring unit (134).

17. The mounting apparatus (100) according to claim 2, comprising a receiving unit (126) configured to align and receive the sample separation unit (30) for fluid coupling between the first fluid port (102) and the second fluid port (106).

18. The installation device (100) according to claim 17, wherein the receiving unit (126) is a column bed.

19. The mounting device (100) according to claim 1, wherein The fluid coupling mechanism (112) is configured at the fluid coupling position to form a high-pressure sealed fluid coupling between the first fluid port (102) and the first fluid connection point (104).

20. The mounting device (100) according to claim 2, wherein The fluid coupling mechanism (112) is configured to form a high-pressure sealed fluid coupling between the first fluid port (102) and the first fluid connection point (104) and between the second fluid port (106) and the second fluid connection point (108).

21. The mounting device (100) according to claim 2, comprising at least one of the following features: wherein The actuation unit (110) and the fluid coupling mechanism (112) are configured to move the actuation unit (110) on the one hand when transferring between the entry position and the fluid coupling position in mutually parallel motion directions (170, 172), and on the other hand, to move relative to each other between the first fluid port (102) and the second fluid port (106); The actuation unit (110) and the fluid coupling mechanism (112) are configured to move the actuation unit (110) in mutually orthogonal motion directions (170, 176) on the one hand when transferring the installation device (100) between the entry position and the fluid coupling position, and on the other hand, to move relative to each other between the first fluid port (102) and the second fluid port (106).

22. The mounting device (100) according to claim 2, comprising at least one of the following features: wherein The first fluid port (102) and the second fluid port (106) are configured to fluidly connect to a horizontally oriented sample separation unit (30). The first fluid port (102) and the second fluid port (106) are configured to fluidly connect to a vertically oriented sample separation unit (30).

23. The mounting device (100) according to claim 1, wherein The actuation unit (110) and / or the fluid coupling mechanism (112) are configured to output feedback when the transfer is completed.

24. The mounting device (100) according to claim 23, wherein the feedback is tactile feedback.

25. A sample separation device (10) for separating a fluid sample in a mobile phase, wherein, The sample separation device (10) includes: A fluid delivery unit (20) is used to deliver the mobile phase and the fluid sample; Sample separation unit (30), which is used to separate the fluid sample in the mobile phase; and According to any one of the installation devices (100) from 1 to 24, the sample separation unit (30) is fluidly installed in the sample separation device (10) via the installation device.

26. The sample separation apparatus (10) according to claim 25, further comprising at least one of the following features: The sample separation unit (30) is configured as a chromatographic separation unit; The sample separation device (10) is configured to analyze at least one physical, chemical and / or biological parameter of at least one component of the fluid sample; The sample separation device (10) includes at least one of the following: apparatus for chemical, biological and / or pharmaceutical analysis, liquid chromatography apparatus, gas chromatography apparatus and HPLC apparatus; The fluid delivery unit (20) is configured to drive the fluid phase at high pressure; The fluid delivery unit (20) is configured to drive the flow phase at a pressure of at least 100 bar; The sample separation device (10) is configured as a microfluidic device; The sample separation device (10) is configured as a nanofluid device; The sample separation device (10) includes a detector (50) for detecting the separated sample. The sample separation device (10) includes an injector (40) for injecting the fluid sample into the mobile phase. The sample separation device (10) includes a sample fractionator (60) for fractionating the separated sample.

27. The sample separation device (10) according to claim 26, wherein the chromatographic separation unit (30) is a chromatographic separation column.

28. The sample separation apparatus (10) according to claim 26, wherein the fluid delivery unit (20) is configured to drive the mobile phase at a pressure of at least 500 bar.

29. The sample separation apparatus (10) according to claim 26, wherein the fluid delivery unit (20) is configured to drive the mobile phase at a pressure of at least 1000 bar.

30. A method for installing a sample separation unit (30) for separating a fluid sample in a sample separation apparatus (10), wherein, The method includes: The sample separation unit (30) is moved from an access position, allowing the user to approach the sample separation unit (30), to a fluid coupling position, whereby the sample separation unit (30) is fluidly coupled within the sample separation device (10), by the actuation unit (110) of the user-actuated installation device (100); and The fluid coupling mechanism (112) is activated by actuating the actuation unit (110) to move the sample separation unit (30) to the fluid coupling position, thereby simultaneously forming a force-loaded fluid coupling of the sample separation unit (30) in the sample separation device (10). The actuation unit (110) includes a pull-out member (114), and the method further includes forming a fluid coupling of the sample separation unit (30) in the sample separation device (10) by the movement of the pull-out member (114).

31. An installation device (100) for installing a sample separation unit (30) for separating fluid samples in a sample separation device (10), wherein, The mounting device (100) includes: A housing (116) including an interior; The first fluid port (102) is used for fluid connection to the first fluid connection point (104) of the sample separation unit (30). An actuation unit (110) capable of being actuated by a user to move between an access position that allows the user to approach the sample separation unit (30) and a fluidly coupled position inside the sample separation unit (30) that prevents the user from approaching it; and A fluid coupling mechanism (112) is configured to form a force-loaded fluid coupling between the first fluid port (102) and the first fluid connection point (104) when the sample separation unit (30) is transferred from the actuation unit (110) to the fluid coupling position.

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