Application of aloe vera whole leaf powder or its active ingredients in preparing medicine for treating chronic atrophic gastritis

Abstract

The invention belongs to the field of traditional Chinese medicine, and discloses the application of aloe leaf powder in the preparation of a drug for treating chronic atrophic gastritis. Aloe leaf powder can significantly improve the phenotype of reduced number of stomach parietal cells, glandular dilatation, increased neck mucous cells and reduced chief cells in mice induced by tamoxifen, and has the efficacy of treating chronic atrophic gastritis. The invention discloses the application of at least one of aloe polysaccharide, aloe polyphenol or aloe flavonoid in the preparation of a drug for treating chronic atrophic gastritis. The polysaccharide, flavonoid and polyphenol in the aloe leaf powder all have the efficacy of treating chronic atrophic gastritis, and the ratio of polysaccharide>polyphenol>flavonoid. The invention discloses a composition for treating chronic atrophic gastritis, which is a composition of polysaccharide, polyphenol and flavonoid according to the mass ratio of (11.247-65.32):(0.373-4.551):(0.452-7.182), which can significantly improve the efficacy of chronic atrophic gastritis and has a synergistic effect.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine, and relates to application of aloe whole leaf powder or its active ingredients in preparing medicine for treating chronic atrophic gastritis, a composition of aloe polysaccharide, aloe polyphenol and aloe flavonoids prepared from aloe whole leaf powder, and application of the composition in preparing medicine for treating chronic atrophic gastritis. Technical Background

[0002] Chronic atrophic gastritis (CAG) is a common digestive disease in clinical practice. Its main clinical manifestations are decreased gastric mucosal parietal cells, wider gastric glandular lumen, and decreased gastric glandular chief cells. It is closely related to Helicobacter pylori infection, age, smoking, drinking and other factors. Its incidence and detection rate increase with age. Modern medicine treats CAG mainly by eradicating Helicobacter pylori (Hp) infection, strengthening barrier function, reducing bile reflux, promoting epithelial growth, and surgical treatment to improve and alleviate patient symptoms. Although the above treatment methods have certain effects, they are still not ideal and have a high recurrence rate. Chronic atrophic gastritis is also a common disease of gastric precancerous lesions. Therefore, the development of an effective drug for the treatment of chronic atrophic gastritis has important scientific research significance and application value.

[0003] Aloe vera is an evergreen fleshy herb of the genus Aloe in the Liliaceae family. It is a medicinal plant that integrates edible, medicinal, and beauty and health care. Among them, Aloe vera is the most widely used aloe variety. About 98.5% to 99.5% of the content is water, and the remaining ingredients are very complex, with about 200 chemical components, mainly including carbohydrates, amino acids, vitamins, minerals, organic acids, lignin, phenolic compounds, anthraquinones and phytosterols. Rich natural active ingredients enable it to improve intestinal absorption, promote wound healing, regulate the body's immunity, anti-cancer, anti-viral, anti-bacterial, anti-inflammatory, antioxidant, whitening and moisturizing and many other effects. At present, no research has shown that aloe vera has the effect of treating chronic atrophic gastritis, and there is no relevant report on its active substance group against chronic atrophic gastritis. Summary of the invention

[0004] The inventors used tamoxifen to construct a mouse chronic atrophic gastritis model, which showed a phenotype of disappearance of parietal cells, glandular expansion, increased neck mucous cells, and decreased chief cells, which is consistent with the pathological characteristics of clinical chronic atrophic gastritis patients.

[0005] The inventors used this model to screen the activity of aloe vera whole leaf powder in treating chronic atrophy. The results showed that aloe vera whole leaf powder can significantly improve the phenotype of tamoxifen-induced reduction in the number of gastric parietal cells, glandular dilatation, increased neck mucous cells, and decreased chief cells in mice, proving that aloe vera whole leaf powder has the efficacy of treating chronic atrophic gastritis. In order to further clarify the active ingredients in aloe vera whole leaf powder for treating chronic atrophic gastritis, the inventors used aloe vera whole leaf powder as raw material to obtain high-purity polysaccharides, flavonoids and polyphenols, and used the tamoxifen-induced mouse chronic atrophic gastritis model to test the activity of the above ingredients. The results showed that the polysaccharides, flavonoids and polyphenols in aloe vera whole leaf powder all have the efficacy of treating chronic atrophic gastritis, and polysaccharides>polyphenols>flavonoids.

[0006] Further based on the theory of Chinese medicine compatibility, it is believed that different medicinal materials can fully exert synergistic effects and improve the efficacy according to the ratio of "monarch, minister, assistant and messenger". The theory of component Chinese medicine believes that the major categories of components in Chinese medicinal materials can be synergistically improved according to the theory of "monarch, minister, assistant and messenger". The inventors combined the above components and screened out an active composition with significant efficacy in improving chronic atrophic gastritis, and the activity was significantly higher than that of a single component, showing synergistic effects.

[0007] The invention aims to provide application of aloe vera whole leaf powder in preparing medicine for treating chronic atrophic gastritis. The aloe vera whole leaf powder is solid powder obtained by dehydrating aloe vera whole leaves.

[0008] The aloe vera whole leaf powder contains polysaccharides, flavonoids and polyphenols.

[0009] Another object of the present invention is to provide the use of at least one of aloe polysaccharide, aloe polyphenol or aloe flavonoid in the preparation of a drug for treating chronic atrophic gastritis.

[0010] The aloe polysaccharide is prepared by the following method: according to the mass volume ratio of aloe whole leaf powder to 80% ethanol of 1:8kg / L, aloe whole leaf powder is added to 80% ethanol, standing at 4°C overnight, centrifuging, discarding the supernatant, and precipitating and vacuum drying to obtain crude polysaccharide I; adding distilled water to dissolve the crude polysaccharide I, adding 0.1-0.2% papain by mass of the crude polysaccharide I, heating to 60°C and stirring for 6h, centrifuging, taking the supernatant, and obtaining crude polysaccharide II after protein removal; D101 macroporous adsorption resin is wet-loaded, crude polysaccharide II is prepared into a sample solution with a mass fraction of 20% by using distilled water, and the crude polysaccharide II is loaded on a D101 macroporous adsorption resin column, and eluted with 50% ethanol of the same volume as the sample solution, recovering ethanol from the eluate until there is no alcohol taste, concentrating in a water bath, and vacuum drying to obtain aloe polysaccharide.

[0011] The aloe polyphenol is prepared by the following method: according to the mass volume ratio of aloe whole leaf powder to 80% ethanol of 1:20kg / L, the aloe whole leaf powder is dissolved in 60% ethanol, ultrasonicated at 60°C for 45 minutes, filtered to obtain an extract, and the extract is concentrated into a solid powder; according to the mass volume ratio of solid powder to 60% ethanol of 1:2kg / L, the solid powder is dissolved in 60% ethanol, an appropriate amount of CaCl2 solution is added until no precipitation is precipitated, the precipitate is collected, hydrochloric acid is added until the precipitate is completely dissolved, ethyl acetate is extracted, the organic phase is collected, and concentrated to obtain the aloe polyphenol.

[0012] The aloe flavonoids are prepared by the following method: according to the mass volume ratio of aloe whole leaf powder to 80% ethanol of 1:8kg / L, the aloe whole leaf powder is added to 80% ethanol, centrifuged, the supernatant is taken, and the sample is added to a D101 macroporous adsorption resin column, distilled water with a volume twice the volume of the sample liquid is used for elution and impurity removal, and then 80% ethanol is used for elution until no flavonoid reaction occurs, the 80% ethanol eluate is collected, ethanol is recovered from the 80% ethanol eluate until there is no alcohol taste, concentrated in a water bath, and vacuum dried to obtain total flavonoids.

[0013] Another object of the present invention is to provide a composition for treating chronic atrophic gastritis, wherein the composition has a mass ratio of polysaccharide, flavonoids and polyphenols of (11.247-65.32):(0.373-4.551):(0.452-7.182).

[0014] Preferably, the mass ratios of polysaccharides, flavonoids and polyphenols are 11.247:0.373:0.452 and 65.32:4.551:7.182.

[0015] Another object of the present invention is to provide the use of the composition in preparing a drug for treating chronic atrophic gastritis.

[0016] Another object of the present invention is to provide a medicine for treating chronic atrophic gastritis, wherein the medicine is prepared from the composition as a main active ingredient and a pharmaceutically acceptable carrier.

[0017] The dosage forms are tablets and capsules. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The results of Aloe vera whole leaf powder on tamoxifen-induced chronic atrophic gastritis in mice.

[0019] Figure 2 The results of the polysaccharides, polyphenols and flavonoids isolated from aloe vera whole leaf powder on tamoxifen-induced chronic atrophic gastritis in mice were presented.

[0020] Figure 3The results of different proportions of polysaccharides, flavonoids and polyphenols isolated from aloe whole leaf powder on the treatment of tamoxifen-induced chronic atrophic gastritis in mice.

[0021] Figure 4 The mathematical model predicts the results of treating atrophic gastritis with different proportions of polysaccharides, polyphenols and flavonoids; Figure A is the contour map of the mathematical model, and Figure B is the 3D response surface of the mathematical model. DETAILED DESCRIPTION

[0022] The following examples are given to further illustrate the technical solution of the present invention. The examples are illustrative and should not be construed as limiting the present invention.

[0023] The aloe vera whole leaf powder used in the embodiment is decolorized aloe vera whole leaf powder (Hainan Zhongchen Bioengineering Co., Ltd.), which is a solid powder of dehydrated aloe vera whole leaf.

[0024] Example 1

[0025] Aloe vera whole leaf powder was administered by gavage to a tamoxifen-induced chronic atrophic gastritis mouse model, and the changes in the number of parietal cells, chief cells and glands in gastric tissue pathological sections were observed to determine whether aloe vera whole leaf powder has therapeutic activity for chronic atrophic gastritis.

[0026] Experimental design: After 7 days of adaptive feeding, C57 / BL mice were randomly placed in cages and divided into 3 groups: Control group, Model group, and whole leaf powder group, with 2 cages in each group and 4 mice in each cage. The mice in the Model group and the whole leaf powder group were gavaged with 250 mg / kg tamoxifen (prepared with a mixed solvent of anhydrous ethanol and sunflower oil in a volume ratio of 1:9, the same below) for the first 3 days. From the 4th day, the mice in the Model group and the whole leaf powder group were gavaged with 62.5 mg / kg (1 / 4 of the initial dose) of tamoxifen. At the same time, the mice in the whole leaf powder group were given aloe whole leaf powder (3.9 g / kg of raw medicinal materials, prepared with a mixed solvent of anhydrous ethanol and sunflower oil in a volume ratio of 1:9). The time interval between drug administration and modeling should not be less than 4 hours, and it lasted for 15 days; the mice in the Control group were gavaged with blank solution (a mixed solvent of anhydrous ethanol and sunflower oil in a volume ratio of 1:9) every day. During the experiment, the body weight and food intake of the mice were recorded every day. After the administration, the stomach tissues were collected for paraffin sectioning and H&E staining.

[0027] Experimental results: Pathological results are shown in Figure 1, showing that compared with the Control group, the gastric mucosal surface of the mice in the Model group and the whole leaf powder group was rough and uneven, with granular appearance, and the parietal cells disappeared. At the same time, the gastric mucosal parietal cells were significantly reduced, and the glands were dilated. Compared with the Model group, after the administration of aloe vera whole leaf powder, the gastric mucosal parietal cells of the mice were significantly restored, and the atrophic glands in the field of vision were significantly reduced. Although the lumen of the mucosal glands was wide and the gland atrophy did not improve significantly, the aloe vera whole leaf powder had the effect of improving chronic atrophic gastritis in general.

[0028] Example 2

[0029] High-purity aloe polysaccharides, aloe flavonoids and aloe polyphenols were extracted using aloe whole leaf powder as raw material. The polysaccharides, flavonoids and polyphenols were applied to the tamoxifen-induced chronic atrophic gastritis mouse model, and the changes in the number of parietal cells, chief cells and glands in the gastric tissue pathological sections were observed to determine whether the polysaccharides, flavonoids and polyphenols in aloe whole leaf powder have the efficacy in treating chronic atrophic gastritis.

[0030] Preparation of aloe flavonoids: according to the mass volume ratio of aloe whole leaf powder and 80% ethanol of 1:8kg / L, the aloe whole leaf powder is added to 80% ethanol, centrifuged at 1350r / min for 10min, the supernatant is taken, and the sample is loaded onto a D101 macroporous adsorption resin column, and the impurities are eluted with distilled water with a volume twice the volume of the loading solution (i.e., the supernatant), and then eluted with 80% ethanol until there is no flavonoid reaction, the 80% ethanol eluate is collected, and the ethanol is recovered from the 80% ethanol eluate until there is no alcohol taste, concentrated in a water bath, and vacuum dried to obtain total flavonoids.

[0031] Determination of aloe flavonoids content:

[0032] Accurately weigh 1.0 g of total flavonoids (dried to constant weight at 120 °C) and add methanol to prepare a 1 g / mL test sample stock solution.

[0033] Preparation of colorimetric reagent: Accurately weigh 1.34 g of AlCl3, dilute to 100 mL with methanol, and prepare a 0.1 mol / L AlCl3 solution. Accurately weigh 2.72 g of CH3COONa·3H2O, dissolve to 100 mL with double distilled water, and prepare a 0.2 mol / L sodium acetate solution. Accurately measure 1.15 mL of glacial acetic acid, dilute to 100 mL with double distilled water, and prepare a 0.2 mol / L acetic acid solution test solution.

[0034] Accurately measure 1mL of the mother solution of the test sample, place it in a 10mL volumetric flask, add 2mL of 0.1mol / L AlCl3 solution, dilute with methanol, fully color for 20min, and record the absorbance value at 270nm. Prepare a standard solution with rutin as the standard substance (the standard solution is 0.025g rutin dissolved in methanol and dilute to 50mL), absorb different amounts of the standard solution, determine the absorbance value through the optimized experimental steps, and use the least squares method for linear fitting to obtain the relationship between rutin concentration C and absorbance value A; the absorbance value shows a good linear relationship in the range of 0.1 to 0.7A. The aloe flavonoid content was measured to be 90.2%.

[0035] Preparation of aloe polysaccharide:

[0036] According to the mass volume ratio of aloe leaf powder and 80% ethanol of 1:8kg / L, aloe leaf powder was added to 80% ethanol, left to stand overnight at 4°C, centrifuged at 1350r / min for 10min, the supernatant was discarded, the precipitate was vacuum dried to obtain crude polysaccharide I; crude polysaccharide I was completely dissolved by adding distilled water, 0.1% papain by mass of crude polysaccharide I was added, heated to 60°C and stirred for 6h, centrifuged, and the supernatant was taken to obtain crude polysaccharide after protein removal, which was recorded as crude polysaccharide II. D101 macroporous adsorption resin was wet-loaded, crude polysaccharide II was weighed, and a sample solution of 20% by mass was prepared with distilled water, which was added to the D101 macroporous adsorption resin column, and eluted with 50% ethanol of the same volume as the sample solution, with a flow rate of 1 / 2 of the sample solution volume / hour, and the eluent recovered ethanol until there was no alcohol taste, concentrated in a water bath, and vacuum dried to obtain aloe polysaccharide.

[0037] Determination of aloe polysaccharide content:

[0038] Accurately weigh 50 mg of glucose (dried to constant weight at 105°C) and place it in a 500-mL volumetric flask. Use distilled water to prepare glucose standard solutions with concentrations of 0.02, 0.04, 0.06, 0.08, and 0.1 mg / mL. Take 1 mL of distilled water and 1 mL of glucose standard solutions of different concentrations, respectively, add 1 mL of 5% phenol solution, and quickly add 5 mL of concentrated sulfuric acid. Let stand for 10 min, shake well, and place at 30°C for 30 min. Then measure the OD value at 490 nm. Use the glucose content as the horizontal axis and the OD value as the vertical axis to prepare a standard curve.

[0039] Take aloe polysaccharide, use distilled water to prepare a sample solution with a concentration of 0.1 mg / mL, draw 1.0 mL of the sample solution, measure OD 490nm according to the above steps, and substitute into the standard curve to calculate the total sugar content of the sample. The aloe polysaccharide content is measured to be 86.4%.

[0040] Extraction of Aloe Polyphenols:

[0041] According to the mass volume ratio of aloe leaf powder to 80% ethanol of 1:20kg / L, the aloe leaf powder is dissolved in 60% ethanol, ultrasonicated at 60°C for 45 minutes, filtered to obtain an extract, and the extract is concentrated into a solid powder; according to the mass volume ratio of solid powder to 60% ethanol of 1:2kg / L, the solid powder is dissolved in 60% ethanol, an appropriate amount of CaCl2 solution is added until no precipitate is precipitated, the precipitate is collected, and 1M hydrochloric acid is slowly added until the precipitate is completely dissolved, extracted with ethyl acetate, the organic phase is collected, and concentrated to obtain aloe polyphenols.

[0042] Determination of the content of aloe polyphenols:

[0043] Accurately weigh 5.00 mg of gallic acid standard, place it in a 50 mL volumetric flask, dilute to the mark with 60% ethanol, shake well, and obtain a gallic acid standard solution with a concentration of 0.1 mg / mL. Accurately pipette 0 mL, 0.1 mL, 0.2 mL, 0.3 mL, 0.4 mL, 0.5 mL, 0.6 mL, and 0.7 mL of gallic acid standard solution into a 10 mL volumetric flask, add 0.5 mL of Folin phenol reagent, and then add 1.5 mL of 1 M Na2CO3 solution, dilute to volume with distilled water, heat in a 75 ° C water bath for 10 minutes, cool naturally, and perform a full wavelength scan. The maximum absorption is at 760 nm. Draw a standard curve with gallic acid concentration (c) as the horizontal axis and absorbance (A) at 760 nm as the vertical axis, and fit the linear regression equation: A = 121c-0.0562, R 2 =0.9996. The content of aloe polyphenols was measured to be 89.9%.

[0044] Experimental design: After 7 days of adaptive feeding, C57 / BL mice were randomly placed in cages and divided into 5 groups, namely: Control group, Model group, polysaccharide group (3.9g / kg raw medicinal material dosage), flavonoid group (3.9g / kg raw medicinal material dosage), polyphenol group (3.9g / kg raw medicinal material dosage), 2 cages in each group, 4 mice in each cage. The Model group, polysaccharide group, flavonoid group, and polyphenol group were gavaged with 250mg / kg tamoxifen for the first 3 days. From the 4th day, the Model group and the three drug-administered groups were gavaged with 62.5mg / kg (1 / 4 of the initial dose) of tamoxifen. At the same time, the three drug-administered groups were given corresponding extraction parts (all prepared with a mixed solvent of anhydrous ethanol and sunflower seed oil in a volume ratio of 1:9), and the time interval between drug administration and modeling should not be less than 4h, which lasted for 15 days; the Control group was gavaged with blank solution (a mixed solvent of anhydrous ethanol and sunflower seed oil in a volume ratio of 1:9) every day. During the experiment, the weight and food intake of mice were recorded every day. After the administration, the stomach tissue was taken for paraffin section and H&E staining.

[0045] Experimental results: Pathological results are shown in Figure 2After 15 days of administration, compared with the Model group, the gastric mucosal parietal cells of the mice in the polysaccharide group and the polyphenol group recovered more completely, and there was no glandular atrophy in the visual field. The gastric mucosal parietal cells of the mice in the flavonoid group recovered completely. Although there was still glandular atrophy in the visual field, it had been significantly improved. This shows that the polysaccharides, flavonoids and polyphenols prepared with aloe vera whole leaf powder as raw materials can significantly improve tamoxifen-induced chronic atrophic gastritis in mice, and the efficacy of polysaccharides>polyphenols>flavonoids.

[0046] Example 3

[0047] The effect of the combination of aloe polysaccharides, aloe flavonoids and aloe polyphenols prepared in Example 2 (compatibility ratio 1, the mass ratio of polysaccharides, flavonoids and polyphenols = 11.247:0.373:0.452, i.e. 93.17:3.090:3.744; compatibility ratio 2, the mass ratio of polysaccharides, flavonoids and polyphenols = 65.32:4.551:7.182, i.e. 84.77:5.906:9.321) on the tamoxifen-induced chronic atrophic gastritis mouse model was investigated.

[0048] Experimental design: After 7 days of adaptive feeding, C57 / BL mice were randomly placed in cages and divided into 4 groups, namely: Control group, Model group, compatibility ratio group 1, and compatibility ratio group 2, with 2 cages in each group and 4 mice in each cage. The Model group, compatibility ratio group 1, and compatibility ratio group 2 mice were gavaged with 250 mg / kg tamoxifen for the first 3 days. From the 4th day, the Model group and the two dosing groups were gavaged with 62.5 mg / kg (initial dose 1 / 4) of tamoxifen. At the same time, the two dosing groups were given the corresponding composition (the total amount of the composition was consistent with the dosage of aloe vera whole leaf powder in Example 1, and both were prepared with a mixed solvent of anhydrous ethanol and sunflower seed oil in a volume ratio of 1:9), and the time interval between dosing and modeling should not be less than 4h, and it lasted for 15 days. The Control group was gavaged with a blank solution (a mixed solvent of anhydrous ethanol and sunflower seed oil in a volume ratio of 1:9) every day. During the experiment, the weight and food intake of mice were recorded every day. After the administration, the stomach tissues were collected for paraffin sectioning and H&E staining.

[0049] Experimental results: Pathological results are shown in Figure 3 After 15 days of administration, compared with the Model group, the pathological section results of the combination ratio group 1 and the combination ratio group 2 showed that the parietal cells were basically restored, and the atrophic glands within the field of view basically disappeared. Both combinations showed a strong effect in improving chronic atrophic gastritis, and the activity was significantly higher than that of a single component.

[0050] Example 4

[0051] The aloe polysaccharide, aloe flavonoids and aloe polyphenols prepared in Example 2 were used as compatibility units, and the polysaccharide, flavonoids and polyphenols were blended into a corresponding mass ratio, and the polysaccharide, flavonoids and polyphenols were applied to a tamoxifen-induced mouse model, and only the number of changes in gastric mucosal parietal cells was used as an indicator for detection. This example uses the D-optimal design method, and the response surface curve method is used to construct a 3D model while keeping the total amount consistent to obtain the optimal drug efficacy compatibility ratio.

[0052] Experimental design: After 7 days of adaptive feeding, C57 / BL mice were randomly placed in cages and divided into 13 groups (see Table 1), with 2 cages in each group and 4 mice in each cage. The 13 groups were named Control group, Model group, and compatibility ratio group 3 to compatibility ratio group 13. The Model group and compatibility ratio groups 1 to 11 were gavaged with 250 mg / kg tamoxifen for the first 3 days. From the 4th day, the Model group and the 11 drug-administered groups were gavaged with 62.5 mg / kg (1 / 4 of the initial dose). At the same time, the 11 drug-administered groups were given the corresponding composition (the total amount of the composition was consistent with the dosage of aloe vera whole leaf powder in Example 1, and all were prepared with a mixed solvent of anhydrous ethanol and sunflower seed oil in a volume ratio of 1:9). The time interval between administration and modeling must not be less than 4 hours, and it lasts for 15 days. The Control group was gavaged with a blank solution (a mixed solvent of anhydrous ethanol and sunflower seed oil in a volume ratio of 1:9) every day. During the experiment, the weight and food intake of the mice were recorded every day. After the administration, the stomach tissues were collected for paraffin sectioning and H&E staining.

[0053] Table 1. Different mass ratios of polysaccharides, flavonoids and polyphenols

[0054]

[0055] Experimental results: See the results Figure 4 Different compatibility ratios had different effects on the number of gastric parietal cells in tamoxifen-induced mice. The constructed model found that when the mass ratio of polysaccharides, flavonoids and polyphenols was 11.247-65.32:0.373-4.551:0.452-7.182, there was a synergistic effect.

Claims

1. A composition for treating chronic atrophic gastritis, characterized in that: The composition is a composition of aloe polysaccharide, aloe flavonoids and aloe polyphenols in a mass ratio of (11.247-65.32):(0.373-4.551):(0.452-7.182); The aloe polysaccharide is prepared by the following method: according to the mass volume ratio of aloe whole leaf powder to 80% ethanol of 1:8kg / L, aloe whole leaf powder is added to 80% ethanol, standing at 4°C overnight, centrifuging, and precipitating in vacuum drying to obtain crude polysaccharide I; crude polysaccharide I is dissolved in distilled water, 0.1-0.2% papain by mass of crude polysaccharide I is added, heated to 60°C and stirred for 6h, centrifuged, and supernatant is taken to obtain crude polysaccharide II; D101 macroporous adsorption resin is wet-loaded, crude polysaccharide II is prepared into a sample solution with a mass fraction of 20% by distilled water, applied to a D101 macroporous adsorption resin column, eluted with 50% ethanol of the same volume as the sample solution, ethanol is recovered from the eluate until there is no alcohol taste, concentrated in a water bath, and vacuum dried to obtain aloe polysaccharide; The aloe polyphenol is prepared by the following method: according to the mass volume ratio of aloe whole leaf powder to 80% ethanol of 1:20kg / L, the aloe whole leaf powder is dissolved in 60% ethanol, ultrasonicated at 60°C for 45 minutes, filtered to obtain an extract, and the extract is concentrated into a solid powder; according to the mass volume ratio of solid powder to 60% ethanol of 1:2kg / L, the solid powder is dissolved in 60% ethanol, an appropriate amount of CaCl2 solution is added until no precipitation is precipitated, the precipitate is collected, hydrochloric acid is added until the precipitate is completely dissolved, extracted with ethyl acetate, the organic phase is collected, and concentrated to obtain aloe polyphenol; The aloe flavonoids are prepared by the following method: according to the mass volume ratio of aloe whole leaf powder to 80% ethanol of 1:8 kg / L, the aloe whole leaf powder is added to 80% ethanol, centrifuged, the supernatant is taken, and the sample is added to a D101 macroporous adsorption resin column, distilled water with a volume twice the volume of the sample liquid is used for elution and impurity removal, and then 80% ethanol is used for elution until no flavonoid reaction occurs, the 80% ethanol eluate is collected, ethanol is recovered from the 80% ethanol eluate until there is no alcohol taste, concentrated in a water bath, and vacuum dried to obtain total flavonoids.

2. The composition according to claim 1, characterized in that: The mass ratios of aloe polysaccharides, aloe flavonoids and aloe polyphenols are 11.247:0.373:0.452 and 65.32:4.551:7.

182.

3. Use of the composition according to claim 1 in the preparation of a medicament for treating chronic atrophic gastritis.

4. A medicine for treating chronic atrophic gastritis, characterized in that: The medicine is prepared by using the composition according to claim 1 as an active ingredient and a pharmaceutically acceptable carrier.

5. The drug according to claim 4, characterized in that: The dosage forms are tablets and capsules.

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