A scalp care composition with oil control and anti-dandruff effects and its application

Abstract

The present invention belongs to the technical field of cosmetics, and particularly relates to a scalp care composition with oil control and anti-dandruff effects and its application. The scalp care composition with oil control and anti-dandruff effects comprises the following components in percentage by mass: 1-5% of capryloyl glycine; 80-94% of green tea fermentation broth; and 5-15% of solubilizer. The scalp care composition prepared by the present invention has certain effects such as oil control and antioxidant. When used on hair, it can preferably reduce the secretion of scalp oil, thereby reducing seborrheic dermatitis and dandruff, and achieving the effects of oil control and anti-dandruff. At the same time, compared with traditional hair products, the scalp care composition of the present invention can effectively solve the problems that excessive use of surfactants will bring a tight feeling to the scalp, cause hair dryness, frizz and other problems.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a scalp care composition with oil control and anti-dandruff effects and its application. Background Art

[0002] The surface of the human scalp and its internal structure can form a complete ecosystem. The surface of the scalp contains colonized bacteria and fungi (such as Staphylococcus epidermidis, Propionibacterium acnes, Malassezia, etc.), sebum secretions, dirt, dust, etc. The sebaceous glands of the scalp are present in the dermis. The sebaceous glands can secrete sebum, which is then discharged to the scalp surface through ducts. Generally, there are a lot of lipids produced by the disintegration of keratinocytes on the scalp surface, and they are mixed to form the so-called "scalp oiliness". In some people, the sebum secretion of the scalp is too strong, which affects the beauty of the hair, and washing the hair too frequently is also likely to damage the hair and scalp. In addition, excessive sebum secretion is likely to cause excessive proliferation of microorganisms such as Malassezia and Propionibacterium acnes on the scalp. These microorganisms not only cause dandruff, but also decompose sebum to produce free fatty acids and other metabolites, causing an inflammatory reaction on the scalp, bringing a series of problems such as scalp itching, irritation, and sensitivity, resulting in damage to the scalp barrier function, and in severe cases, seborrheic dermatitis, hair loss, etc.

[0003] Currently, people usually use oil-control shampoos to slow down the oiliness problem of the scalp. Oil-control shampoos usually increase the addition amount of surfactants to enhance the degreasing power, thereby reducing the oiliness of the scalp. However, using too many surfactants will bring a sense of tightness to the scalp, cause the hair to be dry, frizzy, and other problems. Using too many surfactants can only reduce the oiliness problem of the scalp in a short time and cannot control oil effectively for a long time. After using the product for a period of time, a large amount of sebum is easily generated on the scalp, further exacerbating problems such as seborrheic dermatitis. Summary of the Invention

[0004] The present invention aims to provide a scalp care composition with oil control and anti-dandruff effects and its application. The scalp care composition with oil control and anti-dandruff effects prepared by the present invention has certain oil control, antioxidant and other effects. When used on the hair, it can preferably reduce the secretion of scalp oil, and then reduce the occurrence of seborrheic dermatitis and dandruff, achieving the effects of oil control and anti-dandruff. At the same time, compared with traditional hair products, the scalp care composition with oil control and anti-dandruff effects of the present invention can effectively solve the problems that using too many surfactants will bring a sense of tightness to the scalp, cause the hair to be dry, frizzy, and other problems.

[0005] In order to achieve the above object, the present invention adopts the following technical scheme: A scalp care composition with oil control and anti-dandruff effects, comprising the following components in percentage by mass: 1-5% of capryloyl glycine, 80-94% of green tea fermentation broth, and 5-15% of solubilizer.

[0006] Preferably, the scalp care composition with oil control and anti-dandruff effects comprises the following components by mass percentage: 3% of capryloyl glycine, 87% of green tea fermentation broth, and 10% of solubilizer.

[0007] Preferably, the preparation method of the green tea fermentation broth comprises the following steps: inoculating a lactic acid bacteria seed solution into a fermentation medium containing green tea powder, and obtaining the green tea fermentation broth through fermentation culture, sterilization, and filtration.

[0008] More preferably, the preparation method of the green tea fermentation broth comprises the following steps:

[0009] S1. Preparation of the fermentation medium;

[0010] S2. Preparation of the lactic acid bacteria seed solution: separately preparing a Lactobacillus plantarum seed solution and a Lactobacillus johnsonii seed solution;

[0011] S3. Preparation of the green tea fermentation broth: inoculating the lactic acid bacteria seed solution into the fermentation medium, and obtaining the green tea fermentation broth through fermentation culture, sterilization, and filtration.

[0012] Preferably, the total inoculation amount of the lactic acid bacteria seed solution is 1-3%.

[0013] More preferably, the total inoculation amount of the lactic acid bacteria seed solution is 2%.

[0014] Preferably, the lactic acid bacteria seed solution is a Lactobacillus plantarum seed solution and a Lactobacillus johnsonii seed solution.

[0015] Preferably, the inoculation ratio of the Lactobacillus plantarum seed solution to the Lactobacillus johnsonii seed solution is (2-4):1. More preferably, the inoculation ratio of the Lactobacillus plantarum seed solution to the Lactobacillus johnsonii seed solution is 3:1.

[0016] Preferably, the concentration of the Lactobacillus plantarum seed solution is 1×10 7 ~1×10 9 CFU / mL, and the concentration of the Lactobacillus johnsonii seed solution is 1×10 7 ~1×10 9 CFU / mL.

[0017] More preferably, the concentration of the Lactobacillus plantarum seed solution is 1×10 8 CFU / mL, and the concentration of the Lactobacillus johnsonii seed solution is 1×10 8 CFU / mL.

[0018] Preferably, the preparation method of the Lactobacillus plantarum seed solution is:

[0019] Dissolve Lactobacillus plantarum in sterile water to make a bacterial suspension. Pipette 1 - 5 μL of the Lactobacillus plantarum suspension and streak it on the surface of a solid medium. Activate and culture it at 33 - 42 °C for 24 - 48 h. After the culture, the activated Lactobacillus plantarum is obtained. Inoculate the activated Lactobacillus plantarum into a liquid medium for subculture to obtain the Lactobacillus plantarum seed liquid.

[0020] Preferably, the preparation method of the Lactobacillus johnsonii seed liquid is as follows:

[0021] Dissolve Lactobacillus johnsonii in sterile water to make a bacterial suspension. Pipette 1 - 5 μL of the Lactobacillus johnsonii suspension and streak it on the surface of a solid medium. Activate and culture it at 33 - 42 °C for 24 - 48 h. After the culture, the activated Lactobacillus johnsonii is obtained. Inoculate the activated Lactobacillus johnsonii into a liquid medium for subculture to obtain the Lactobacillus johnsonii seed liquid.

[0022] More preferably, in the present invention, the solid medium selected in the preparation processes of the Lactobacillus plantarum seed liquid and the Lactobacillus johnsonii seed liquid is MRS solid medium, and the liquid medium selected is MRS liquid medium.

[0023] More preferably, in the preparation processes of the Lactobacillus plantarum seed liquid and the Lactobacillus johnsonii seed liquid, the conditions for subculture are a temperature of 33 - 42 °C, a rotation speed of 100 - 200 rpm, and a time of 24 - 48 h.

[0024] Preferably, the fermentation medium comprises the following components by mass percentage: green tea powder 0.5 - 2%, carbon source 0.5 - 3%, nitrogen source 0.5 - 2%, inorganic salts 0.05 - 0.5%, and the balance water.

[0025] More preferably, the fermentation medium comprises the following components by mass percentage: green tea powder 1.2%, carbon source 1%, nitrogen source 0.8%, inorganic salts 0.2%, and the balance water.

[0026] Preferably, the carbon source includes at least one of glucose, fructose syrup, and maltose.

[0027] More preferably, the carbon source is selected from fructose syrup.

[0028] Preferably, the nitrogen source includes at least one of yeast extract, soy peptone, tryptone, and ammonium sulfate.

[0029] More preferably, the nitrogen source is selected from yeast extract.

[0030] In the present invention, the selection of the carbon source and the nitrogen source will both affect the fermentation process of the green tea fermentation broth, resulting in differences in the active substances of the prepared green tea fermentation broth, and ultimately affecting the oil control and anti-dandruff effects of the scalp care composition.

[0031] Preferably, the inorganic salt includes at least one of phosphate and sulfate.

[0032] More preferably, the inorganic salt is selected from sodium dihydrogen phosphate.

[0033] Preferably, the method for preparing the fermentation medium is as follows: mix green tea powder, carbon source, nitrogen source, inorganic salt and water evenly, keep warm at 120 - 125 °C for 30 - 50 min, and then cool to obtain the fermentation medium.

[0034] Preferably, the conditions for fermentation culture are: ferment and culture at a temperature of 33 - 42 °C and a rotation speed of 80 - 180 rpm for 36 - 60 h.

[0035] More preferably, the conditions for fermentation culture are: ferment and culture at a temperature of 37 °C and a rotation speed of 120 rpm for 48 h.

[0036] Preferably, the conditions for sterilization are: keep warm at 85 - 105 °C for 10 - 40 min to obtain the sterilized fermentation broth.

[0037] Preferably, the conditions for filtration are: filter the sterilized fermentation broth with diatomaceous earth to obtain the green tea fermentation broth.

[0038] Preferably, the solubilizer includes at least one of propylene glycol, glycerol, and hydrogenated castor oil CO - 40.

[0039] The green tea fermentation broth in the present invention has certain effects such as oil control and antioxidant; capryloyl glycine has the functions of oil control, bacteriostasis and promoting the absorption of other active substances. The applicant has found that combining capryloyl glycine with the green tea fermentation broth fermented under specific conditions can effectively promote the transdermal absorption of the active substances in the green tea fermentation broth, and when used on the hair, it can preferably reduce the secretion of scalp oil, thereby reducing seborrheic dermatitis and dandruff, achieving the effects of oil control and anti - dandruff.

[0040] The present invention also claims the use of the scalp care composition having the effects of oil control and anti - dandruff in the preparation of hair products.

[0041] Preferably, the dosage forms of the hair products include solutions, suspensions, emulsions, pastes, gels, creams, powders, soaps, sprays, oils.

[0042] The present invention also claims a hair product, comprising 0.1 - 20% by mass of the scalp care composition having the effects of oil control and anti - dandruff.

[0043] Compared with the prior art, the present invention has the following beneficial effects:

[0044] Through the interaction between the green tea fermentation broth prepared by fermentation under specific conditions and components such as capryloyl glycine, the present invention can effectively enhance the functions of oil control, antioxidant, etc. Applying it to hair products can better reduce the secretion of scalp oil, thereby reducing the occurrence of seborrheic dermatitis and dandruff, achieving the effects of oil control and anti-dandruff. At the same time, the preparation method of the present invention is simple, which is conducive to large-scale industrial production. Detailed implementation manners

[0045] The following will combine the embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0046] In the examples and comparative examples, unless otherwise specified, the experimental methods used are all conventional methods, and the materials, reagents, etc. used, unless otherwise specified, can all be obtained from commercial channels.

[0047] The resource number of the Lactobacillus plantarum selected in the examples and comparative examples of the present invention is BNCC194165, which is purchased from Beijing Beina Chuanglian Biotechnology Research Institute;

[0048] The resource number of the Lactobacillus johnsonii is BNCC135265, which is purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0049] The preparation method of the green tea powder selected in the examples of the present invention is as follows:

[0050] Select dry green tea leaves, grind them with a high-speed powder grinder, and sieve the obtained powder through a 60-100 mesh sieve. Take the lower-layer powder as the green tea powder for fermentation.

[0051] The preparation method of the MRS solid medium selected in the examples and comparative examples of the present invention is (taking the preparation of 1L as an example):

[0052] Mix 10 g of peptone, 5 g of beef powder, 4 g of yeast powder, 2 g of glucose, 1 mL of Tween 80, 2 g of dipotassium hydrogen phosphate, 5 g of sodium acetate, 2 g of ammonium citrate, 0.2 g of magnesium sulfate, 0.05 g of manganese sulfate, and 15 g of agar powder, add distilled water to 1L, sterilize at 120 °C for 20 min, and store for later use.

[0053] The preparation method of the MRS liquid medium selected in the examples and comparative examples of the present invention is (taking the preparation of 1L as an example):

[0054] Mix 10 g of peptone, 5 g of beef powder, 4 g of yeast powder, 2 g of glucose, 1 mL of Tween 80, 2 g of dipotassium hydrogen phosphate, 5 g of sodium acetate, 2 g of ammonium citrate, 0.2 g of magnesium sulfate, and 0.05 g of manganese sulfate, and add distilled water to make up to 1 L. Sterilize at 120 °C for 20 min and store for later use.

[0055] Example 1

[0056] Formula of the scalp care composition with oil control and anti-dandruff effects: by mass percentage, 3% of octanoyl glycine, 87% of green tea fermentation broth, and 10% of glycerol.

[0057] The preparation method of the green tea fermentation broth is as follows:

[0058] S1. Preparation of the fermentation medium: by mass percentage, mix 1.2% of green tea powder, 1% of fructose syrup, 0.8% of yeast extract, 0.2% of sodium dihydrogen phosphate, and the balance of water evenly, keep warm at 121 °C for 30 min, and cool to obtain the fermentation medium;

[0059] S2. Preparation of the lactic acid bacteria seed liquid: Prepare the Lactobacillus plantarum seed liquid and the Lactobacillus johnsonii seed liquid respectively;

[0060] The preparation method of the Lactobacillus plantarum seed liquid: Dissolve Lactobacillus plantarum in sterile water to obtain a bacterial suspension. Pipette 3 μL of the Lactobacillus plantarum suspension onto the MRS solid medium and activate and culture at 35 °C for 36 h to obtain activated Lactobacillus plantarum; Inoculate the activated Lactobacillus plantarum into the MRS liquid medium and expand the culture at 35 °C and a rotation speed of 150 rpm for 36 h to obtain a Lactobacillus plantarum seed liquid with a bacterial concentration of about 1×10 8 CFU / mL;

[0061] The preparation method of the Lactobacillus johnsonii seed liquid: Dissolve Lactobacillus johnsonii in sterile water to obtain a bacterial suspension. Pipette 3 μL of the Lactobacillus johnsonii suspension onto the MRS solid medium and activate and culture at 35 °C for 36 h to obtain activated Lactobacillus johnsonii; Inoculate the activated Lactobacillus johnsonii into the MRS liquid medium and expand the culture at 35 °C and a rotation speed of 150 rpm for 36 h to obtain a Lactobacillus johnsonii seed liquid with a bacterial concentration of about 1×10 8 CFU / mL;

[0062] S3. Preparation of the green tea fermentation broth:

[0063] Inoculate the Lactobacillus plantarum seed solution (inoculation amount 1.5%) and the Lactobacillus johnsonii seed solution (inoculation amount 0.5%) into the fermentation medium, and ferment and culture at 37°C and a rotation speed of 120 rpm for 48 h to obtain a fermentation broth; keep the fermentation broth at 90°C for 20 min to obtain the sterilized fermentation broth; filter the sterilized fermentation broth with a diatomite filter to obtain a green tea fermentation broth.

[0064] Example 2

[0065] Formulation of a scalp care composition with oil control and anti-dandruff effects: By mass percentage, 5% octanoyl glycine, 85% green tea fermentation broth, and 10% glycerol.

[0066] The preparation method of the green tea fermentation broth is the same as that in Example 1.

[0067] Example 3

[0068] Formulation of a scalp care composition with oil control and anti-dandruff effects: By mass percentage, 1% octanoyl glycine, 89% green tea fermentation broth, and 10% glycerol.

[0069] The preparation method of the green tea fermentation broth is the same as that in Example 1.

[0070] Example 4

[0071] Formulation of a scalp care composition with oil control and anti-dandruff effects: By mass percentage, 3% octanoyl glycine, 87% green tea fermentation broth, and 10% glycerol.

[0072] Among them, the preparation steps of the green tea fermentation broth are similar to those in Example 1, except that an equal amount of glucose is selected to replace fructose syrup, and glucose is used as the carbon source of the fermentation substrate.

[0073] Example 5

[0074] Formulation of a scalp care composition with oil control and anti-dandruff effects: By mass percentage, 3% octanoyl glycine, 87% green tea fermentation broth, and 10% glycerol.

[0075] Among them, the preparation steps of the green tea fermentation broth are similar to those in Example 1, except that an equal amount of soy peptone is selected to replace yeast extract, and yeast extract is used as the nitrogen source of the fermentation substrate.

[0076] Example 6

[0077] Formulation of a scalp care composition with oil control and anti-dandruff effects: By mass percentage, 3% octanoyl glycine, 87% green tea fermentation broth, and 10% glycerol.

[0078] Among them, the preparation steps of the green tea fermentation broth are similar to those of Example 1, except that the inoculation amount of Lactobacillus plantarum is 1.6% and the inoculation amount of Lactobacillus johnsonii is 0.4%.

[0079] Example 7

[0080] Formulation of a scalp care composition with oil control and anti-dandruff effects: by mass percentage, 1% octanoyl glycine, 94% green tea fermentation broth, and 5% propylene glycol.

[0081] Among them, the preparation method of the green tea fermentation broth is as follows:

[0082] S1. Preparation of the fermentation medium: By mass percentage, 2.0% green tea powder, 0.8% maltose, 2% ammonium sulfate, 0.1% magnesium sulfate, and the balance of water are mixed evenly, kept at 121 °C for 30 min, and cooled to obtain the fermentation medium;

[0083] S2. Preparation of the lactic acid bacteria seed liquid: Prepare the Lactobacillus plantarum seed liquid and the Lactobacillus johnsonii seed liquid respectively;

[0084] Dissolve Lactobacillus plantarum in sterile water to obtain a bacterial suspension. Pipette 5 μL of the Lactobacillus plantarum suspension onto an MRS solid medium and activate it at 38 °C for 48 h to obtain activated Lactobacillus plantarum; inoculate the activated Lactobacillus plantarum into an MRS liquid medium and culture it at 38 °C and a rotation speed of 100 rpm for 48 h to obtain a Lactobacillus plantarum seed liquid with a bacterial concentration of about 1×10 9 CFU / mL;

[0085] Dissolve Lactobacillus johnsonii in sterile water to obtain a bacterial suspension. Pipette 5 μL of the Lactobacillus johnsonii suspension onto an MRS solid medium and activate it at 38 °C for 48 h to obtain activated Lactobacillus johnsonii; inoculate the activated Lactobacillus johnsonii into an MRS liquid medium and culture it at 38 °C and a rotation speed of 100 rpm for 48 h to obtain a Lactobacillus johnsonii seed liquid with a bacterial concentration of about 1×10 9 CFU / mL;

[0086] S3. Preparation of the green tea fermentation broth:

[0087] Inoculate the Lactobacillus plantarum seed liquid (inoculation amount 2%) and the Lactobacillus johnsonii seed liquid (inoculation amount 1%) into the fermentation medium, and ferment and culture it at 35 °C and a rotation speed of 180 rpm for 36 h to obtain a fermentation broth; keep the fermentation broth at 105 °C for 10 min to obtain a sterilized fermentation broth; filter the sterilized fermentation broth with a diatomaceous earth filter to obtain the green tea fermentation broth.

[0088] Example 8

[0089] Formulation of a scalp care composition with oil control and anti-dandruff effects: By mass percentage, 5% capryloyl glycine, 80% green tea fermentation broth, and 15% hydrogenated castor oil - CO - 40.

[0090] Among them, the preparation method of the green tea fermentation broth is as follows:

[0091] S1. Preparation of the fermentation medium: By mass percentage, mix 0.5% green tea powder, 3% fructose syrup, 0.5% tryptone, 0.5% dipotassium hydrogen phosphate, and the balance of water evenly, keep it at 121 °C for 30 min, and obtain the fermentation medium after cooling;

[0092] S2. Preparation of the lactic acid bacteria seed liquid: Prepare the Lactobacillus plantarum seed liquid and the Lactobacillus johnsonii seed liquid respectively;

[0093] Dissolve Lactobacillus plantarum in sterile water to obtain a bacterial suspension. Pipette 1 μL of the Lactobacillus plantarum suspension onto an MRS solid medium, and activate and culture it at 33 °C for 24 h to obtain activated Lactobacillus plantarum; inoculate the activated Lactobacillus plantarum into an MRS liquid medium, and expand the culture at 33 °C and a rotation speed of 200 rpm for 24 h to obtain a Lactobacillus plantarum seed liquid with a bacterial concentration of about 1×10 7 CFU / mL;

[0094] Dissolve Lactobacillus johnsonii in sterile water to obtain a bacterial suspension. Pipette 1 μL of the Lactobacillus johnsonii suspension onto an MRS solid medium, and activate and culture it at 33 °C for 24 h to obtain activated Lactobacillus johnsonii; inoculate the activated Lactobacillus johnsonii into an MRS liquid medium, and expand the culture at 33 °C and a rotation speed of 200 rpm for 24 h to obtain a Lactobacillus johnsonii seed liquid with a bacterial concentration of about 1×10 7 CFU / mL;

[0095] S3. Preparation of the green tea fermentation broth:

[0096] Inoculate the Lactobacillus plantarum seed liquid (inoculation amount 0.75%) and the Lactobacillus johnsonii seed liquid (inoculation amount 0.25%) into the fermentation medium, and ferment and culture it at 37 °C and a rotation speed of 80 rpm for 60 h to obtain a fermentation broth; keep the fermentation broth at 85 °C for 40 min to obtain a sterilized fermentation broth; filter the sterilized fermentation broth with a diatomaceous earth filter to obtain the green tea fermentation broth.

[0097] Comparative Example 1

[0098] Formulation of the scalp care composition: By mass percentage, 90% green tea fermentation broth and 10% glycerol.

[0099] Compared with Example 1, the difference in this comparative example is only that capryloyl glycine is not added.

[0100] The preparation method of the green tea fermentation broth is the same as that in Example 1.

[0101] Comparative Example 2

[0102] The formulation of the scalp care composition: by mass percentage, 3% of capryloyl glycine, 87% of deionized water, and 10% of glycerol.

[0103] Compared with Example 1, the difference in this comparative example is only that the green tea fermentation broth is replaced with an equal amount of deionized water.

[0104] Comparative Example 3

[0105] The formulation of the scalp care composition: by mass percentage, 3% of capryloyl glycine, 87% of green tea extract, and 10% of glycerol.

[0106] Among them, the preparation method of the green tea extract is as follows:

[0107] By mass percentage, 1.2% of green tea powder is mixed evenly with the remaining amount of water, and extracted for 48 h under the stirring conditions of 80 °C and 120 rpm to obtain an extract, and the extract is filtered with a diatomite filter to obtain the green tea extract.

[0108] Comparative Example 4

[0109] The formulation of the scalp care composition: by mass percentage, 0.5% of capryloyl glycine, 89.5% of green tea fermentation broth, and 10% of glycerol.

[0110] The preparation method of the green tea fermentation broth is the same as that in Example 1.

[0111] Comparative Example 5

[0112] The formulation of the scalp care composition: by mass percentage, 6% of capryloyl glycine, 84% of green tea fermentation broth, and 10% of glycerol.

[0113] The preparation method of the green tea fermentation broth is the same as that in Example 1.

[0114] Comparative Example 6

[0115] The formulation of the scalp care composition: by mass percentage, 3% of capryloyl glycine, 87% of green tea fermentation broth, and 10% of glycerol.

[0116] The preparation method of the green tea fermentation broth:

[0117] S1. Preparation of the fermentation medium: By mass percentage, 1.2% of green tea powder, 1% of fructose syrup, 0.8% of yeast extract, 0.2% of sodium dihydrogen phosphate, and the remaining amount of water are mixed evenly, kept at 121 °C for 30 min, and cooled to obtain the fermentation medium;

[0118] S2. Preparation of lactic acid bacteria seed liquid: Prepare Lactobacillus plantarum seed liquid, and the preparation method is the same as that in Example 1;

[0119] S3. Preparation of green tea fermentation broth:

[0120] Inoculate the Lactobacillus plantarum seed liquid (inoculation amount 2.0%) into the fermentation medium, and ferment and culture at 37°C and a rotation speed of 120 rpm for 48 h to obtain a fermentation broth; keep the fermentation broth at 90°C for 20 min to obtain a sterilized fermentation broth; filter the sterilized fermentation broth with a diatomaceous earth filter to obtain green tea fermentation broth.

[0121] Compared with Example 1, the difference in this comparative example is only that the green tea fermentation broth is obtained only by fermentation with a single Lactobacillus plantarum during the preparation process.

[0122] Comparative Example 7

[0123] Formulation of the scalp care composition: By mass percentage, 3% octanoyl glycine, 87% green tea fermentation broth, and 10% glycerol.

[0124] Preparation method of green tea fermentation broth:

[0125] S1. Preparation of fermentation medium: By mass percentage, mix 1.2% green tea powder, 1% fructose syrup, 0.8% yeast extract, 0.2% sodium dihydrogen phosphate and the remaining water evenly, keep at 121°C for 30 min, and cool to obtain the fermentation medium;

[0126] S2. Preparation of lactic acid bacteria seed liquid: Prepare Lactobacillus johnsonii seed liquid, and the preparation method is the same as that in Example 1;

[0127] S3. Preparation of green tea fermentation broth:

[0128] Inoculate the Lactobacillus johnsonii seed liquid (inoculation amount 2.0%) into the fermentation medium, and ferment and culture at 37°C and a rotation speed of 120 rpm for 48 h to obtain a fermentation broth; keep the fermentation broth at 90°C for 20 min to obtain a sterilized fermentation broth; filter the sterilized fermentation broth with a diatomaceous earth filter to obtain green tea fermentation broth.

[0129] Compared with Example 1, the difference in this comparative example is only that the green tea fermentation broth is obtained only by fermentation with a single Lactobacillus johnsonii seed liquid during the preparation process.

[0130] Comparative Example 8

[0131] Formulation of the scalp care composition: By mass percentage, 3% octanoyl glycine, 87% green tea fermentation broth, and 10% glycerol.

[0132] Compared with Example 1, the difference in this comparative example is only that the inoculation amount of Lactobacillus plantarum seed liquid is 1% and the inoculation amount of Lactobacillus johnsonii seed liquid is 1% during the preparation of green tea fermentation broth.

[0133] Comparative Example 9

[0134] Formulation of the scalp care composition: 3% octanoyl glycine, 87% green tea fermentation broth, and 10% glycerol by mass percentage.

[0135] Among them, the preparation steps of the green tea fermentation broth are similar to those in Example 1, and the difference is only that an equal amount of dextrin is used to replace fructose syrup, and dextrin is used as the carbon source of the fermentation substrate.

[0136] Test Example 1. Scalp oil control and anti-dandruff effect test

[0137] I. Test samples: Shampoo prepared with the scalp care compositions prepared in Examples 1-8 and Comparative Examples 1-9 as active substances. The formulation components of the shampoo are shown in Table 1.

[0138] Table 1

[0139]

[0140] Preparation method of the shampoo:

[0141] After mixing the materials in Phase A evenly until transparent, add the materials in Phase B, mix evenly, heat to 65 °C, and keep warm for standby. Under the condition of 65 °C, add the materials in Phase C, mix evenly, heat to 80 °C, and keep warm for standby. Under the condition of 80 °C, add the materials in Phase D, mix evenly, keep warm for 15 min, and defoam; cool down to 45 °C, add the materials in Phase E, mix evenly; add the materials in Phase F, mix evenly; add the materials in Phase G, mix evenly to prepare the shampoo.

[0142] The formulation components of the matrix shampoo are similar to those of the shampoo, and the difference is only that an equal amount of deionized water is used to replace the scalp care composition.

[0143] The preparation method is the same as that of the shampoo.

[0144] II. Test method

[0145] 180 volunteers with oily scalps and dandruff were selected and divided into 18 groups. Each group of 10 volunteers used a shampoo. Before the volunteers used the test samples, a 2-week washout period was set to avoid the influence of their original hair products deposited on the hair and scalp. Based on the consistency of the washout products, the non-conditioning Johnson's Baby Shampoo & Body Wash was used as the washout agent, used once every 2 or 3 days. After the 2-week washout period ended, the volunteers used the test samples provided by the present invention according to their personal habits, with a usage amount of at least the size of a one-yuan coin each time. Follow-up visits were conducted at the beginning of use and 4 weeks after use.

[0146] Test indicators:

[0147] Oil control effect indicator: Before using the test samples and 4 weeks after using the test samples, the sebum secretion amount on the top of the volunteers' scalps was measured. The oil control effect of the product was judged by the change rate of the sebum secretion amount. If the change rate of the sebum secretion amount is negative 4 weeks after using the test product, it indicates that the sebum secretion amount is less, and the test product has an oil control effect. The larger the negative value of the change rate of the sebum secretion amount, the better the oil control effect of the test product.

[0148] Change rate of sebum secretion amount (%) = (Sebum secretion amount 4 weeks after use - Sebum secretion amount before use) / Sebum secretion amount before use.

[0149] Test instrument: Skin sebum tester (Sebumeter SM815).

[0150] Anti-dandruff effect indicator:

[0151] Before using the test samples and 4 weeks after using the test samples, photos of the top of the volunteers' heads were taken with a camera, and professional testers evaluated whether the dandruff situation of the volunteers improved, so as to judge the anti-dandruff effect of the shampoo.

[0152] Dandruff evaluation method: Before using the test samples, 3 areas with the most severe dandruff on the top of the volunteers' heads were selected, and the dandruff indices of the 3 areas were evaluated. The scoring of the dandruff index is as follows in the table. Each area was scored 2 - 3 times, and the average value of the scoring results of the three areas was taken to obtain the pre-use dandruff index score value. 4 weeks after using the test samples, the dandruff index of the volunteers was scored in the same way, and the average value of the scoring results of the three areas was taken to obtain the post-use dandruff index score value. The change rate of the dandruff index score values before and after using the test samples was compared to judge the anti-dandruff effect of the shampoo.

[0153] Table 2 Anti-dandruff effect judgment criteria

[0154] Degree None Very Slight Slight Moderate Severe Very Severe Score 0 1 2 3 4 5

[0155] Test instruments: PhotoMax PRO lens, Nikon D500 camera.

[0156] Rate of change of dandruff index score (%) = (dandruff index score after 4 weeks of use - dandruff index score before use) / dandruff index score before use * 100%.

[0157] A negative rate of change of the dandruff index score (%) indicates that the dandruff condition of the volunteers has improved. Moreover, the larger the negative value, the better the dandruff improvement effect and the better the anti-dandruff effect of the shampoo.

[0158] III. Experimental results

[0159] The test results are shown in Tables 3 and 4.

[0160] Table 3 Oil control effect of each sample

[0161]

[0162]

[0163] Table 4 Anti-dandruff effect of each sample

[0164] Test Sample Before Use 4 Weeks after Use Rate of Change Matrix Shampoo 4.57 4.82 5.47 Example 1 4.62 2.56 -44.59 Example 2 4.51 2.61 -42.13 Example 3 4.67 2.93 -37.26 Example 4 4.58 2.82 -38.43 Example 5 4.52 2.75 -39.16 Example 6 4.70 2.67 -43.19 Example 7 4.48 2.82 -37.05 Example 8 4.62 2.96 -35.93 Comparative Example 1 4.65 4.37 -6.02 Comparative Example 2 4.49 4.02 -10.47 Comparative Example 3 4.50 3.98 -11.56 Comparative Example 4 4.61 3.91 -15.18 Comparative Example 5 4.58 3.67 -19.87 Comparative Example 6 4.47 3.73 -16.55 Comparative Example 7 4.61 3.92 -14.97 Comparative Example 8 4.46 3.35 -24.89 Comparative Example 9 4.63 3.58 -22.68

[0165] From the data in Tables 3 and 4, it can be seen that the matrix shampoo has no oil control effect, and the dandruff condition becomes more serious after using the matrix shampoo. The shampoos of the examples and comparative examples all have certain oil control and anti-dandruff effects, and the oil control and anti-dandruff effects of the examples are better than those of the comparative examples.

[0166] Among them, the oil control and anti-dandruff effects of Examples 1-8 are better than those of Comparative Examples 1-2, indicating that the composition formed by capryloyl glycine and green tea fermentation broth of the present invention has a synergistic effect. Replacing capryloyl glycine with the same mass of green tea fermentation broth results in a poorer oil control and anti-dandruff effect of the formed composition. The present invention discovers that the combined use of capryloyl glycine and the green tea fermentation broth provided by the present invention has a better oil control effect than the oil control and anti-dandruff effects of single components. The oil control and anti-dandruff effects of Examples 1-8 are better than those of Comparative Example 3, and the oil control and anti-dandruff effects of Comparative Example 3 are only slightly higher than those of Comparative Example 2, indicating that the composition formed by capryloyl glycine and the green tea extract prepared by the simple water extraction method also has a poorer oil control and anti-dandruff effect. The oil control effects of Examples 1-3 are better than those of Comparative Examples 4-5, indicating that the addition ratio of capryloyl glycine and green tea fermentation broth in the formula components has an important influence on the oil control and anti-dandruff effects of the finally prepared product. When the addition ratio of the two components is inappropriate, their oil control and anti-dandruff effects are significantly deteriorated.

[0167] The oil control and anti-dandruff effects of Examples 1 and 6 are superior to those of Comparative Examples 6-8, indicating that both the fermentation strains and the inoculation ratios of the strains during the preparation of the green tea fermentation broth can affect the fermentation process of the green tea fermentation broth, resulting in differences in the active substances of the prepared green tea fermentation broth, and ultimately affecting the oil control and anti-dandruff effects of the composition. In the present invention, the green tea fermentation broth is preferably fermented symbiotically by Lactobacillus plantarum and Lactobacillus johnsonii, and when the inoculation ratio of the Lactobacillus plantarum seed solution to the Lactobacillus johnsonii seed solution is in the range of (2-4):1, better oil control and anti-dandruff effects can be achieved.

[0168] The oil control and anti-dandruff effects of Examples 1, 4, and 5 are superior to those of Comparative Example 9, indicating that the selection of carbon sources and nitrogen sources during the preparation of the green tea fermentation broth affects the fermentation process of the green tea fermentation broth, resulting in differences in the active substances of the prepared green tea fermentation broth, and ultimately affecting the oil control and anti-dandruff effects of the composition.

[0169] The above examples are only illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Any person familiar with this technology can modify or change the above examples without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by those with ordinary knowledge in the technical field without departing from the spirit and technical ideas disclosed by the present invention should still be covered by the claims of the present invention.

Claims

1. A scalp care composition having the effects of controlling oil and removing dandruff, characterized in that, It comprises components in the following mass percentages: 1-5% of capryloyl glycine, 80-94% of green tea fermentation broth, and 5-15% of solubilizer; the preparation method of the green tea fermentation broth comprises the following steps: inoculating a lactic acid bacteria seed solution into a fermentation medium containing green tea powder, and obtaining the green tea fermentation broth through fermentation culture, sterilization, and filtration; the solubilizer comprises at least one of propylene glycol, glycerol, and hydrogenated castor oil CO-40; the fermentation medium comprises components in the following mass percentages: 0.5-2% of green tea powder, 0.5-3% of carbon source, 0.5-2% of nitrogen source, 0.05-0.5% of inorganic salt, and the balance of water; the carbon source comprises at least one of glucose, fructose syrup, and maltose; the nitrogen source comprises at least one of yeast extract, soy peptone, tryptone, and ammonium sulfate; the inorganic salt comprises at least one of phosphate and sulfate; the conditions for the fermentation culture are fermentation culture for 36-60 h under the conditions of a temperature of 33-42 °C and a rotation speed of 80-180 rpm. The lactic acid bacteria seed solution is a Lactobacillus plantarum seed solution and a Lactobacillus johnsonii seed solution. The total inoculation amount of the lactic acid bacteria seed liquid is 1-3%; the inoculation ratio of the Lactobacillus plantarum seed liquid to the Lactobacillus johnsonii seed liquid is (2-4):1; the concentration of the Lactobacillus plantarum seed liquid is 1×10 7 ~1×10 9 CFU / mL, and the concentration of the Lactobacillus johnsonii seed liquid is 1×10 7 ~1×10 9 CFU / mL; The preparation method of the Lactobacillus plantarum seed solution is as follows: dissolving Lactobacillus plantarum in sterile water to form a bacterial suspension, taking 1-5 μL of the Lactobacillus plantarum suspension and streaking it on the surface of a solid medium, and performing activation culture at 33-42 °C for 24-48 h to obtain activated Lactobacillus plantarum after the culture; inoculating the activated Lactobacillus plantarum into a liquid medium for subculture to obtain the Lactobacillus plantarum seed solution. The preparation method of the Lactobacillus johnsonii seed solution is as follows: dissolving Lactobacillus johnsonii in sterile water to form a bacterial suspension, taking 1-5 μL of the Lactobacillus johnsonii suspension and streaking it on the surface of a solid medium, and performing activation culture at 33-42 °C for 24-48 h to obtain activated Lactobacillus johnsonii after the culture; inoculating the activated Lactobacillus johnsonii into a liquid medium for subculture to obtain the Lactobacillus johnsonii seed solution.

2. The scalp care composition having oil control and anti-dandruff effects according to claim 1, wherein It comprises components in the following mass percentages: 3% of capryloyl glycine, 87% of green tea fermentation broth, and 10% of solubilizer.

3. Use of a scalp care composition having oil control and anti-dandruff effects as described in any one of claims 1 to 2 in the preparation of hair products.

4. A hair product, characterized in that, It comprises 0.1-20% by mass of a scalp care composition having oil control and anti-dandruff effects as described in any one of claims 1 to 2.

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