A Zhuang medicine for relieving vascular dementia and its preparation method

Abstract

The present invention discloses a Zhuangyi Linggui Qiaowu prescription for relieving vascular dementia and a preparation method thereof. The Zhuangyi Linggui Qiaowu prescription is made of a main drug, a supplementary drug and a secondary drug: the raw materials of the main drug include: ganoderma lucidum, angelica vine and centipede; the raw materials of the supplementary drug include: notoginseng, salvia miltiorrhiza, chuanxiong and calamus; the raw materials of the secondary drug include: polygala tenuifolia and arisaema cuneata. The Zhuangyi Linggui Qiaowu prescription obtained by the method of the present invention has the functions of improving cognitive impairment related to hippocampus, reducing neuronal damage in the hippocampus of the brain, and enhancing the short-term memory function of the hippocampus of the brain, thereby relieving vascular dementia caused by various cerebrovascular diseases.

Description

Technical Field

[0001] The invention relates to the field of traditional Chinese medicine, and in particular to a Zhuang medicine for relieving vascular dementia and a preparation method thereof. Background Art

[0002] Vascular dementia (VD) refers to a type of central degenerative disease caused by various cerebrovascular diseases that damage brain tissue and then cause brain intelligence and cognitive dysfunction. The prevalence of vascular dementia in the elderly population over 65 years old is 1.5%. It is the most common dementia disease after Alzheimer's disease, and it is showing an increasing trend year by year with the aging of the population. Studies have found that vascular dementia is reversible in the early stage, and early prevention can significantly improve the prognosis of patients with vascular dementia.

[0003] In recent years, Western medicine has made great progress in improving patients' cognitive impairment and clinical symptoms. However, considering that vascular dementia is more common in the elderly, the elderly generally have weak constitutions and are accompanied by more underlying diseases, and some Western medicines for the treatment of vascular dementia have more side effects and long courses of treatment, which are not friendly to the elderly. Zhuang medicine uses a feasible prescription to relieve vascular dementia, combining tonic and meridian dredging, and targeted conditioning of VD from the inside to the outside, with obvious efficacy and few side effects.

[0004] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0005] The object of the present invention is to provide a strong medicine for alleviating vascular dementia.

[0006] Another object of the present invention is to provide a method for preparing a strong medicine for alleviating vascular dementia.

[0007] To achieve the above-mentioned purpose, the present invention provides a Zhuang medicine for alleviating vascular dementia, characterized in that it is made of a main medicine, an auxiliary medicine and a supporting medicine: the raw materials of the main medicine include: Ganoderma lucidum, Angelica dahurica vine and Scolopendra subspinipes; the raw materials of the auxiliary medicine include: Notoginseng, Salvia miltiorrhiza, Ligusticum chuanxiong and Acorus calamus; the raw materials of the supporting medicine include: Polygala tenuifolia and Arisaema ciliata.

[0008] Preferably, in the above technical solution, the raw materials of the main drug are proportioned by weight as follows: 13-18g of Ganoderma lucidum, 13-18g of Angelica sinensis vine and 8-12g of Scolopendra subspinipes.

[0009] Preferably, in the above technical solution, the raw materials of the main drug are mixed in a weight ratio of 15g of Ganoderma lucidum, 15g of Angelica sinensis vine and 10g of Centipede arborealis.

[0010] Preferably, in the above technical solution, the raw materials of the auxiliary medicine are matched by weight in the following manner: 8-12g of Panax notoginseng, 13-18g of Salvia miltiorrhiza, 8-12g of Ligusticum chuanxiong and 13-18g of Acorus calamus.

[0011] Preferably, in the above technical solution, the raw materials of the auxiliary medicine are matched by weight in the following manner: 10g of Panax notoginseng, 15g of Salvia miltiorrhiza, 10g of Ligusticum chuanxiong and 15g of Acorus calamus.

[0012] Preferably, in the above technical solution, the weight ratio of the medicated raw materials is 8-12g of Polygala tenuifolia and 8-12g of Arisaema ciliata.

[0013] Preferably, in the above technical solution, the weight ratio of the medicated raw materials is 10g of Polygala tenuifolia and 10g of Arisaema ciliata.

[0014] The present application also provides a method for preparing a strong medicine for alleviating vascular dementia, comprising the following steps:

[0015] (1) Medicinal material processing: Weigh the medicinal materials according to the formula, slice the angelica vine and soak it in warm water, slice the arisaema ciliata and boil it with ginger juice over high heat for three times, then simmer it over low heat for several times, boil it again, dry it in the air, and prepare the arisaema ciliata for later use;

[0016] (2) First decoction: For the first decoction, boil the prepared Arisaema confusa with Ganoderma lucidum, Scolopendra subspinipes and Salvia miltiorrhiza in water over high heat and then simmer for 40-47 minutes over low heat. Then add Panax notoginseng, Ligusticum chuanxiong, Acorus calamus and Polygala tenuifolia into the decoction and simmer for 25-30 minutes over low heat. Then pour the soaked Angelica sinensis vine slices and water into the first decoction and simmer for 10-15 minutes over high heat. Filter and pour out the first decoction to obtain the decoction.

[0017] Second decoction: Add appropriate amount of water to the medicinal residue and put it into the decoction container for second decoction. After the second decoction is simmered over high heat for 15-20 minutes, the medicinal liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid is mixed with the second decoction liquid to obtain a Zhuang medicine.

[0018] Preferably, in the above technical solution, the method of using the Zhuang medicine is to take it warm 2-2.5 hours after breakfast and dinner, 260 ml each time.

[0019] The drug mechanism of the present invention is as follows:

[0020] Lingzhi: It tastes sweet and bitter, is neutral in nature, and enters the heart, lung, liver, and spleen meridians; it has the functions of replenishing qi and calming the mind, nourishing the five internal organs, nourishing the heart and calming the mind, nourishing the lungs and replenishing qi, regulating qi and removing blood stasis, and nourishing the liver and spleen; it is mainly used to treat weakness, fatigue, palpitations, insomnia, dizziness, chronic cough and asthma, loss of appetite, slow reaction, shortness of breath, etc. The "germanium" contained in Lingzhi can increase the ability of human blood to absorb oxygen by 1.5 times. Lingzhi contains amino acids, polypeptides, proteins, and a variety of reducing sugars and polysaccharides, ergosterol, triterpenes, coumarin glycosides, volatile oils, stearic acid, benzoic acid, alkaloids, vitamin B2 and vitamin C and other nutrients.

[0021] Angelica vine: bitter and astringent, warm in nature. It enters the liver and kidney meridians, has the effects of promoting blood circulation and dispersing blood stasis, nourishing the kidney and strengthening the waist, nourishing blood, promoting blood circulation, and strengthening the waist and knees. It contains sugars, glycosides, phenols, tannins, saponins, organic acids, flavonoids, triterpenes, volatile oils and fats.

[0022] Tree-climbing centipede: tastes pungent and bitter, and is neutral in nature; it enters the liver, spleen, and lung meridians; it has the effects of dispelling wind, promoting dampness, promoting dampness, harmonizing blood, and detoxifying, and is mainly used to treat rheumatic arthralgia, headache, dizziness, and bruises.

[0023] Ginseng: Sweet and slightly bitter, warm in nature. The main ingredient is ginsenoside, which has the effects of promoting blood circulation and removing blood stasis, replenishing qi and promoting body fluid, dredge meridians and relieve pain, and disperse blood stasis and stop bleeding. It is used to treat swelling and pain from falls, metrorrhagia, cardiotonic, and angina pectoris.

[0024] Chuanxiong: spicy in taste, warm in nature; it enters the liver, gallbladder, and heart meridians, has the effects of promoting blood circulation and qi, dispelling wind and relieving pain, dispersing heat and promoting circulation, promoting blood circulation and removing blood stasis, and regulating the nervous system. It is mainly used to treat coronary heart disease, angina pectoris, migraine, and traumatic injuries.

[0025] Salvia miltiorrhiza: bitter in taste, slightly cold in nature; enters the heart and liver meridians; has the effects of promoting blood circulation and regulating menstruation, removing blood stasis and relieving pain, cooling blood and eliminating carbuncle, clearing the heart and relieving restlessness, nourishing blood and calming the mind, promoting blood circulation and removing blood stasis, clearing the heart and calming the mind, cooling blood and eliminating carbuncle, anti-thrombotic and lipid-lowering. It is mainly used to treat chest and rib pain, rheumatic pain, mass agglomerations, swelling and pain of sores, pain from falls, irregular menstruation, amenorrhea and dysmenorrhea, postpartum blood stasis and pain, etc.

[0026] Acorus calamus: It tastes numbing and bitter, and is warm in nature. It has the effects of regulating the mind, clearing the fire channels, removing dampness and toxins, stimulating the appetite, opening the orifices and resolving phlegm, refreshing the mind and improving intelligence, relieving cough and asthma, and has antibacterial and anti-inflammatory effects. It is mainly used to treat diseases such as phlegm in the mind, insomnia and forgetfulness, tinnitus and deafness, chest tightness and pain, cough and asthma, poor appetite, and Alzheimer's disease.

[0027] Polygala: bitter and pungent in taste, warm in nature; enters the heart, kidney and lung meridians; has the effects of calming the mind and improving intelligence, connecting the heart and kidney, removing phlegm, reducing swelling, calming the mind and improving intelligence, removing phlegm and opening the orifices, and dissipating carbuncles; mainly treats symptoms of insomnia, dreaminess, forgetfulness, palpitations, and trance.

[0028] Arisaema consanguineum: bitter, slightly pungent, and cool in nature; enters the lung, liver, and spleen meridians; has the effects of clearing away heat and resolving phlegm, calming wind and relieving convulsions, clearing heat and resolving phlegm, clearing the heart and calming the nerves, and relieving pain and paroxysms. It is mainly used to treat symptoms such as stroke, epilepsy, convulsions, dizziness caused by headache, cough and wheezing caused by phlegm and fire, stroke and phlegm confusion, and epilepsy.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The principles of the Zhuang medicine drug combination used in the present invention to relieve vascular dementia are as follows:

[0031] (1) Angelica dahurica and Scolopendra subspinipes are representative strong medicines for dredging the dragon path. They have the effects of dredging the dragon path and the fire path, dispelling wind and activating blood circulation, activating blood circulation and dispersing blood stasis, removing the evil wind in the brain blood vessels, meridians and internal organs, relieving aging and hardening of blood vessels, and activating blood circulation and tendons to enhance blood circulation. At the same time, Ganoderma lucidum is a representative strong medicine for tonic. It has the effects of nourishing the five internal organs, replenishing qi and calming the mind, nourishing the liver and strengthening the spleen. It contains a variety of proteins, amino acids and triterpenes. The three together serve as the main medicine. They dredge the dragon path to enhance the heart's pumping ability, maintain smooth nutrient delivery between internal organs, bones and flesh, and dredge the fire path to improve the nerves' ability to receive various external information and stimuli, thereby alleviating brain intelligence and cognitive dysfunction.

[0032] (2) Panax notoginseng contains rich ginsenosides, which have the effects of promoting blood circulation and unblocking collaterals, invigorating qi and promoting the production of body fluids; Chuanxiong has the effects of dispersing heat and promoting circulation, promoting blood circulation and removing blood stasis, and regulating the nervous system; combined with Salvia miltiorrhiza, it has the effects of nourishing blood and calming the mind, promoting blood circulation and removing blood stasis, clearing the heart and calming the mind; and Acorus calamus has the effects of regulating the spleen and stomach, unblocking the fire channels, removing dampness and toxins, stimulating the appetite, opening the orifices and removing phlegm, refreshing the mind and improving intelligence, relieving cough and asthma, and having antibacterial and anti-inflammatory effects. Together, they have the effects of strengthening the heart and unblocking collaterals, and regulating the stability of the nervous system.

[0033] (3) Polygala tenuifolia enters the heart, kidney, and lung meridians, and is a representative strong medicine for the dragon path and the fire path. Arisaema consanguineum enters the lung, liver, and spleen meridians, and is a representative strong medicine for the valley path and the fire path. It can clear phlegm, open the orifices, calm the wind and calm the nerves, and is beneficial for regulating the two paths and guiding all the medicines to the diseased area to relieve the condition.

[0034] The principle of the preparation method of Zhuang medicine used in the present invention to reduce the toxicity of some drugs and increase the precipitation of drugs is as follows: first, angelica vine is sliced ​​and soaked in warm water to make its cells fully absorb water and swell, so that some effective ingredients in the angelica vine slices are dissolved in water, and high-fire decocting is used to prevent the volatile oil in the angelica vine from evaporating; secondly, high-fire decocting and long-term decocting of Arisaema ciliata with ginger juice can reduce the toxicity of Arisaema ciliata, and the method of package decocting is used to prevent the impurities of Arisaema ciliata from mixing into the medicinal solution during the decocting process; Ganoderma lucidum, centipede and salvia miltiorrhiza are tonics, and long-term decocting with low fire is conducive to the full precipitation of the nutrients therein.

[0035] Description of the drawings

[0036] Figure 1This is a picture of the Nissl-stained cells in the CA1 region of the hippocampus of rats in the sham operation group, the model group, and Example 1 in the method of the present invention;

[0037] Figure 2 The pictures are taken of the Nissl-stained cells in the CA1 region of the hippocampus of rats in Comparative Examples 1, 2 and 3 of the method of the present invention;

[0038] Figure 3 These are pictures of Nissl-stained cells in the CA1 region of the hippocampus of rats in Comparative Examples 4 and 5 of the method of the present invention. DETAILED DESCRIPTION

[0039] The specific implementation modes of the present invention are described in detail below in conjunction with specific embodiments, but it should be understood that the protection scope of the present invention is not limited by the specific implementation modes.

[0040] Example 1

[0041] A Zhuang medicine for relieving vascular dementia, comprising a main medicine, an auxiliary medicine and a supporting medicine:

[0042] The raw materials of the main medicine are matched by weight as 15g of Ganoderma lucidum, 15g of Angelica sinensis vine and 10g of centipede; the raw materials of the auxiliary medicine are matched by weight as 10g of Panax notoginseng, 15g of Salvia miltiorrhiza, 10g of Ligusticum chuanxiong and 15g of Acorus calamus; the raw materials of the supporting medicine are matched by weight as 10g of Polygala tenuifolia and 10g of Arisaema ciliata.

[0043] A method for preparing a Zhuang medicine for alleviating vascular dementia, comprising the following specific steps:

[0044] (1) Medicinal material processing: Weigh the medicinal materials according to the formula, slice the angelica vine and soak it in 100 ml of warm water for 40 minutes, slice the arisaema cinerea and boil it with ginger juice over high heat for three times, then simmer it over low heat for several times, boil it again, dry it in the air, and prepare the arisaema cinerea for later use;

[0045] (2) First decoction: For the first decoction, add the processed Arisaema confusa, Ganoderma lucidum, Scolopendra subspinipes and Danshen into 500 ml of water, boil over high heat, then simmer over low heat for 40 min. Then add Panax notoginseng, Chuanxiong, Acorus calamus and Polygala tenuifolia into the decoction and simmer over low heat for 30 min. Then, pour the soaked Angelica sinensis slices and water into the first decoction, simmer over high heat for 15 min, and filter out to obtain the first decoction liquid.

[0046] (3) Second decoction: Add 400 ml of water to the residue and put it into the decoction container for second decoction. After the second decoction is decocted over high heat for 20 minutes, the medicinal liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid and the second decoction liquid are mixed to obtain Zhuang medicine.

[0047] The method of use of this Zhuang medicine is to take it warm 2.5 hours after breakfast and dinner, 260ml each time.

[0048] Example 2

[0049] A Zhuang medicine for relieving vascular dementia, comprising a main medicine, an auxiliary medicine and a supporting medicine:

[0050] The raw materials of the main medicine are matched by weight as 17g of Ganoderma lucidum, 17g of Angelica sinensis vine and 14g of Scolopendra subspinipes; the raw materials of the auxiliary medicine are matched by weight as 14g of Panax notoginseng, 17g of Salvia miltiorrhiza, 14g of Ligusticum chuanxiong and 17g of Acorus calamus; the raw materials of the supporting medicine are matched by weight as 14g of Polygala tenuifolia and 14g of Arisaema ciliata.

[0051] A method for preparing a Zhuang medicine for alleviating vascular dementia, comprising the following specific steps:

[0052] (1) Medicinal material processing: Weigh the medicinal materials according to the formula, slice the angelica vine and soak it in 100 ml of warm water for 40 minutes, slice the arisaema cinerea and boil it with ginger juice over high heat for three times, then simmer it over low heat for several times, boil it again, dry it in the air, and prepare the arisaema cinerea for later use;

[0053] (2) First decoction: For the first decoction, add the processed Arisaema confusa, Ganoderma lucidum, Scolopendra subspinipes and Danshen to 500 ml of water, boil over high heat, then simmer over low heat for 45 min. Then add Panax notoginseng, Chuanxiong, Acorus calamus and Polygala tenuifolia to the decoction and simmer over low heat for 25 min. Then, pour the soaked Angelica sinensis slices and water into the first decoction and simmer over high heat for 10 min. Filter and pour out the first decoction to obtain the decoction.

[0054] (3) Second decoction: Add 450 ml of water to the residue and put it into the decoction container for second decoction. After the second decoction is decocted over high heat for 17 minutes, the medicinal liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid and the second decoction liquid are mixed to obtain Zhuang medicine.

[0055] The method of use of Zhuang medicine is to take it warm 2.7 hours after breakfast and dinner, 260ml each time.

[0056] Example 3

[0057] A Zhuang medicine for relieving vascular dementia, comprising a main medicine, an auxiliary medicine and a supporting medicine:

[0058] The raw materials of the main medicine are 13g of Ganoderma lucidum, 13g of Angelica sinensis vine and 8g of centipede; the raw materials of the auxiliary medicine are 8g of Panax notoginseng, 13g of Salvia miltiorrhiza, 8g of Chuanxiong and 13g of Acorus calamus; the raw materials of the supporting medicine are 8g of Polygala tenuifolia and 8g of Arisaema consanguineum.

[0059] A method for preparing a Zhuang medicine for alleviating vascular dementia, comprising the following specific steps:

[0060] (1) Medicinal material processing: Weigh the medicinal materials according to the formula, slice the angelica vine and soak it in 100 ml of warm water for 40 minutes, slice the arisaema cinerea and boil it with ginger juice over high heat for three times, then simmer it over low heat for several times, boil it again, dry it in the air, and prepare the arisaema cinerea for later use;

[0061] (2) First decoction: For the first decoction, add 500 ml of water to boil the prepared Arisaema confusa, Ganoderma lucidum, Scolopendra subspinipes and Salvia miltiorrhiza to high heat, then simmer for 43 min over low heat. Then add Panax notoginseng, Ligusticum chuanxiong, Acorus calamus and Polygala tenuifolia to the decoction and simmer for 27 min over low heat. Then, pour the soaked Angelica sinensis slices and water into the first decoction and simmer for 13 min over high heat. Filter and pour out the first decoction to obtain the decoction.

[0062] (3) Second decoction: Add 430 ml of water to the residue and put it into the decoction container for second decoction. After the second decoction is decocted over high heat for 15 minutes, the medicinal liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid and the second decoction liquid are mixed to obtain Zhuang medicine.

[0063] The method of use of Zhuang medicine is to take it warm 3 hours after breakfast and dinner, 260ml each time.

[0064] Example 4

[0065] A Zhuang medicine for relieving vascular dementia, comprising a main medicine, an auxiliary medicine and a supporting medicine:

[0066] The raw materials of the main medicine are matched by weight as 16g of Ganoderma lucidum, 16g of Angelica sinensis vine and 12g of Scolopendra subspinipes; the raw materials of the auxiliary medicine are matched by weight as 12g of Panax notoginseng, 16g of Salvia miltiorrhiza, 12g of Ligusticum chuanxiong and 16g of Acorus calamus; the raw materials of the auxiliary medicine are matched by weight as 12g of Polygala tenuifolia and 12g of Arisaema ciliata.

[0067] A method for preparing a Zhuang medicine for alleviating vascular dementia, comprising the following specific steps:

[0068] (1) Medicinal material processing: Weigh the medicinal materials according to the formula, slice the angelica vine and soak them in warm water for a period of time, slice the arisaema ciliata and boil it with ginger juice over high heat for three times, then simmer it over low heat for several times, boil it again, dry it in the air, and prepare the arisaema ciliata for later use;

[0069] (2) First decoction: For the first decoction, boil the prepared Arisaema confusa with Ganoderma lucidum, Scolopendra subspinipes and Salvia miltiorrhiza in water over high heat and then simmer for 47 min over low heat. Then add Panax notoginseng, Ligusticum chuanxiong, Acorus calamus and Polygala tenuifolia into the decoction and simmer for 30 min over low heat. Then pour the soaked Angelica sinensis vine slices and water into the first decoction and simmer for 15 min over high heat. Filter and pour out the first decoction to obtain the decoction.

[0070] (3) Second decoction: Add an appropriate amount of water to the residue and place it in a decoction container for a second decoction. After the second decoction is decocted over high heat for 20 minutes, the medicinal liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid and the second decoction liquid are mixed to obtain Zhuang medicine.

[0071] The method of use of Zhuang medicine is to take it warm 2.8 hours after breakfast and dinner, 260ml each time.

[0072] Example 5

[0073] A Zhuang medicine for relieving vascular dementia, comprising a main medicine, an auxiliary medicine and a supporting medicine:

[0074] The raw materials of the main medicine are matched by weight as 16g of Ganoderma lucidum, 16g of Angelica sinensis vine and 12g of Scolopendra subspinipes; the raw materials of the auxiliary medicine are matched by weight as 12g of Panax notoginseng, 16g of Salvia miltiorrhiza, 12g of Ligusticum chuanxiong and 16g of Acorus calamus; the raw materials of the auxiliary medicine are matched by weight as 12g of Polygala tenuifolia and 12g of Arisaema ciliata.

[0075] A method for preparing a Zhuang medicine for alleviating vascular dementia, comprising the following specific steps:

[0076] (1) Medicinal material processing: Weigh the medicinal materials according to the formula, slice the angelica vine and soak them in warm water for a period of time, slice the arisaema ciliata and boil it with ginger juice over high heat for three times, then simmer it over low heat for several times, boil it again, dry it in the air, and prepare the arisaema ciliata for later use;

[0077] (2) First decoction: For the first decoction, boil the prepared Arisaema confusa with Ganoderma lucidum, Scolopendra subspinipes and Salvia miltiorrhiza in water over high heat and then simmer for 40 min over low heat. Then add Panax notoginseng, Ligusticum chuanxiong, Acorus calamus and Polygala tenuifolia into the decoction and simmer for 25 min over low heat. Then pour the soaked Angelica sinensis vine slices and water into the first decoction and simmer for 10 min over high heat. Filter and pour out the first decoction to obtain the decoction.

[0078] (3) Second decoction: Add an appropriate amount of water to the residue and place it in a decoction container for a second decoction. After the second decoction is decocted over high heat for 15 minutes, the medicinal liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid and the second decoction liquid are mixed to obtain Zhuang medicine.

[0079] The method of use of Zhuang medicine is to take it warm 3 hours after breakfast and dinner, 260ml each time.

[0080] Comparative Example 1

[0081] The invention is basically the same as Example 1, except that the formula of the Zhuang medicine is different. A Zhuang medicine for relieving vascular dementia is prepared from a main medicine and an auxiliary medicine:

[0082] The raw materials of the main medicine are 15g of Ganoderma lucidum, 15g of Angelica sinensis vine and 10g of Scolopendra subspinipes; the raw materials of the auxiliary medicine are 15g of Salvia miltiorrhiza, 10g of Ligusticum chuanxiong and 15g of Acorus tatarinowii.

[0083] A method for preparing a Zhuang medicine for alleviating vascular dementia, comprising the following specific steps:

[0084] (1) Medicinal material processing: Weigh the medicinal materials according to the formula, slice the angelica vine and soak it in 100 ml of warm water for 40 minutes;

[0085] (2) First decoction: For the first decoction, add 500 ml of water to boil the Ganoderma lucidum, Scolopendra subspinipes and Danshen, then simmer for 40 min over high heat. Then add Chuanxiong and Acorus calamus to the decoction and simmer for 30 min over low heat. Then, add the soaked Angelica sinensis slices and water to the first decoction and simmer for 15 min over high heat. Filter and pour out the first decoction to obtain the decoction.

[0086] (3) Second decoction: Add 400 ml of water to the residue and put it into the decoction container for second decoction. After the second decoction is decocted over high heat for 20 minutes, the medicinal liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid and the second decoction liquid are mixed to obtain Zhuang medicine.

[0087] The method of use of this Zhuang medicine is to take it warm 2.5 hours after breakfast and dinner, 260ml each time.

[0088] Comparative Example 2

[0089] The invention is basically the same as Example 1, except that the formula of the Zhuang medicine is different. A Zhuang medicine for relieving vascular dementia is prepared from a main medicine, an auxiliary medicine and a supporting medicine:

[0090] The raw materials of the main medicine are matched by weight to 15g of Ganoderma lucidum and 10g of Scolopendra subspinipes; the raw materials of the auxiliary medicine are matched by weight to 10g of Panax notoginseng, 15g of Salvia miltiorrhiza, 10g of Ligusticum chuanxiong and 15g of Acorus calamus; the raw materials of the auxiliary medicine are matched by weight to 10g of Arisaema consanguineum.

[0091] A method for preparing a Zhuang medicine for alleviating vascular dementia, comprising the following specific steps:

[0092] (1) Medicinal material processing: Weigh the medicinal materials according to the formula, slice the Arisaema consanguineum, boil it with ginger juice over high heat for three times, then boil it over low heat for several times, boil it again, dry it in the air, and prepare the Arisaema consanguineum for later use;

[0093] (2) First decoction: First decoction: Add 500 ml of water to boil the processed Arisaema confusa, Ganoderma lucidum, Scolopendra subspinipes and Danshen, then simmer for 40 min over high heat. Then add Panax notoginseng, Chuanxiong and Acorus calamus into the decoction and simmer for 30 min over low heat. Filter and pour out the first decoction.

[0094] (3) Second decoction: Add 400 ml of water to the residue and put it into the decoction container for second decoction. After the second decoction is decocted over high heat for 20 minutes, the medicinal liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid and the second decoction liquid are mixed to obtain Zhuang medicine.

[0095] The method of use of this Zhuang medicine is to take it warm 2.5 hours after breakfast and dinner, 260ml each time.

[0096] Comparative Example 3

[0097] The same as Example 1, except that the Zhuang medicine formula is different. A Zhuang medicine for relieving vascular dementia, comprising a main medicine, an auxiliary medicine and a carrying medicine:

[0098] The raw materials of the main medicine are 15g of Ganoderma lucidum and 15g of Angelica sinensis vine in a weight ratio; the raw materials of the auxiliary medicine are 10g of Panax notoginseng, 15g of Salvia miltiorrhiza, 10g of Chuanxiong and 15g of Acorus calamus in a weight ratio; the raw materials of the auxiliary medicine are 10g of Polygala tenuifolia in a weight ratio.

[0099] A method for preparing a Zhuang medicine for alleviating vascular dementia, comprising the following specific steps:

[0100] (1) Medicinal material processing: Weigh the medicinal materials according to the formula, slice the angelica vine and soak it in 100 ml of warm water for 40 minutes;

[0101] (2) First decoction: For the first decoction, add 500 ml of water and boil Ganoderma lucidum and Danshen over high heat, then simmer for 40 min over low heat. Then add Radix Notoginseng, Rhizoma Chuanxiong, Acorus calamus and Polygala tenuifolia to the decoction and simmer for 30 min over low heat. Then, add the soaked Angelica sinensis slices and water to the first decoction and simmer for 15 min over high heat. Filter and pour out the first decoction to obtain the decoction.

[0102] (3) Second decoction: Add 400 ml of water to the residue and put it into the decoction container for second decoction. After the second decoction is decocted over high heat for 20 minutes, the medicinal liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid and the second decoction liquid are mixed to obtain Zhuang medicine.

[0103] The method of use of this Zhuang medicine is to take it warm 2.5 hours after breakfast and dinner, 260ml each time.

[0104] Comparative Example 4

[0105] The invention is basically the same as Example 1, except that the formula ratio is different. A Zhuang medicine for relieving vascular dementia is prepared from a main medicine, an auxiliary medicine and a carrying medicine:

[0106] The raw materials of the main medicine are matched by weight as 10g of Ganoderma lucidum, 10g of Angelica sinensis vine and 15g of centipede; the raw materials of the auxiliary medicine are matched by weight as 15g of Panax notoginseng, 10g of Salvia miltiorrhiza, 15g of Ligusticum chuanxiong and 10g of Acorus calamus; the raw materials of the auxiliary medicine are matched by weight as 15g of Polygala tenuifolia and 10g of Arisaema ciliata.

[0107] A method for preparing a Zhuang medicine for alleviating vascular dementia, comprising the following specific steps:

[0108] (1) Medicinal material processing: Weigh the medicinal materials according to the formula, slice the angelica vine and soak it in 100 ml of warm water for 40 minutes, slice the arisaema cinerea and boil it with ginger juice over high heat for three times, then simmer it over low heat for several times, boil it again, dry it in the air, and prepare the arisaema cinerea for later use;

[0109] (2) First decoction: For the first decoction, add the processed Arisaema confusa, Ganoderma lucidum, Scolopendra subspinipes and Danshen into 500 ml of water, boil over high heat, then simmer over low heat for 40 min. Then add Panax notoginseng, Chuanxiong, Acorus calamus and Polygala tenuifolia into the decoction and simmer over low heat for 30 min. Then, pour the soaked Angelica sinensis slices and water into the first decoction, simmer over high heat for 15 min, and filter out to obtain the first decoction liquid.

[0110] (3) Second decoction: Add 400 ml of water to the residue and put it into the decoction container for second decoction. After the second decoction is decocted over high heat for 20 minutes, the medicinal liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid and the second decoction liquid are mixed to obtain Zhuang medicine.

[0111] The method of use of this Zhuang medicine is to take it warm 2.5 hours after breakfast and dinner, 260ml each time.

[0112] Comparative Example 5

[0113] The method is basically the same as Example 1, except that the method for preparing the Zhuang medicine is different. A Zhuang medicine for relieving vascular dementia is prepared from a main medicine, an auxiliary medicine and a carrier medicine:

[0114] The raw materials of the main medicine are matched by weight as 15g of Ganoderma lucidum, 15g of Angelica sinensis vine and 10g of centipede; the raw materials of the auxiliary medicine are matched by weight as 10g of Panax notoginseng, 15g of Salvia miltiorrhiza, 10g of Ligusticum chuanxiong and 15g of Acorus calamus; the raw materials of the supporting medicine are matched by weight as 10g of Polygala tenuifolia and 10g of Arisaema ciliata.

[0115] A method for preparing a Zhuang medicine for alleviating vascular dementia, comprising the following specific steps:

[0116] (1) First decoction: First decoction: Add 500 ml of water to boil the herbs, including Arisaema consanguineum, Ganoderma lucidum, Scolopendra subspinipes and Salvia miltiorrhiza, and simmer for 40 min over high heat. Then add Radix Notoginseng, Rhizoma Chuanxiong, Acorus calamus, Polygala tenuifolia and Radix Angelicae sinensis to the decoction and simmer for 30 min over low heat. Filter and pour out the first decoction.

[0117] (2) Second decoction: Add 400 ml of water to the residue and put it into the decoction container for second decoction. The second decoction is boiled over high heat and then simmered over low heat for 20 minutes. The medicinal liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid and the second decoction liquid are mixed to obtain Zhuang medicine.

[0118] The method of use of this Zhuang medicine is to take it warm 2.5 hours after breakfast and dinner, 260ml each time.

[0119] In order to prove the effectiveness of the Zhuang medicine obtained by the present invention, the applicant has conducted a large number of experimental studies, which are as follows: first, a VD rat model was prepared, and a simulation test was performed using the VD rat model.

[0120] 1. Preparation of VD Rat Model

[0121] The following experiment used the method of bilateral permanent ligation of common carotid arteries (2-VO) to induce chronic forebrain ischemia and prepare VD animal model. The method is as follows: rats fasted for 12 hours before surgery and deprived of water for 4 hours before surgery. After gas induction (isoflurane, concentration 2%, rate 200mL / min) anesthesia, they were fixed on the operating table in supine position. Spontaneous breathing was ensured during the operation. The neck was depilated and disinfected with 2% iodine. The midline of the neck was incised, and the bilateral common carotid arteries were separated. Double "1" line was used, and the proximal and distal ends of the bilateral common carotid arteries were ligated respectively, and the artery was cut from the middle to ensure the blockage of arterial blood flow. 2mL of gentamicin injection was applied externally to the surgical incision, and the incision was sutured. After the operation, the animals were sent to a well-ventilated animal room for feeding. After anesthesia, the sham operation group rats only separated the bilateral common carotid arteries, but did not ligate or cut the arteries.

[0122] 2. Dosage

[0123] One week after rat modeling, the drug was obtained according to the formulation and preparation method of Example 1 and Comparative Examples 1-5, and the obtained drug was given to the VD model rats of the corresponding groups at 10 mL·kg -1 Standard administration; the sham operation group and the model group were given 10 mL kg -1 Distilled water was given intragastricly; rats in each group were gavaged once within 2.5-3 hours after meals in the morning and evening every day for 4 weeks.

[0124] 3. Test types, methods, data and conclusions

[0125] (1) Morris water maze test: The rats in each group were subjected to Morris water maze test after 4 weeks of intervention. The pool was 150 cm in diameter and 60 cm in height, and was completely black. Edible melanin was added to the water to make the pool water black and opaque. The circular platform was 10 cm in diameter and its surface was black. The platform surface was submerged 2 cm under the water. The water temperature was controlled at 24±1℃. Orientation navigation test: The rats were placed in the water from four quadrants randomly with their ventral side facing the wall of the bucket. The time to find the platform within 90 s was recorded (escape latency: the time required for the animal to find and climb onto the platform after entering the water maze). If the rat found the platform within 90 s, it was allowed to stay on the platform for 10 s, and the actual escape latency within 90 s was recorded. If the rat did not find the platform, it was guided to the platform and stayed for 10 s. The escape latency was recorded as 90 s. The interval between each entry into the water was 1 min. The test lasted for 5 days. Spatial exploration test: On the 6th day of testing, the hidden platform was removed and rats from each group were placed in the quadrant opposite to the platform. The number of times the rats crossed the platform area was recorded. The results are shown in Table 1.

[0126] Table 1 Comparison of escape latency time of rats in each group

[0127]

[0128]

[0129] Note: * Indicates P < 0.05 compared with the sham operation group; # Indicates P < 0.05 compared with the model group.

[0130] Morris water maze detection method is mainly used to evaluate the cognitive impairment and degree of hippocampus-related cognitive function, and is a classic method for studying the spatial memory ability of AD transgenic animals. The results are shown in Table 1. In Example 1 of the Zhuang medicine obtained by the prescription of the present invention, compared with Comparative Examples 1-3, the time required for the escape latency of the VD model rats in Example 1 is gradually shortened as the experiment proceeds, indicating that the use of the method of the present invention can alleviate the degree of cognitive impairment related to the hippocampus in the VD model rats, and further illustrates that the Zhuang medicine prescription using the method of the present invention can improve the symptoms of brain intelligence and cognitive dysfunction caused by vascular dementia; In Example 1 of the Zhuang medicine obtained by the ratio of the present invention, compared with Comparative Example 4, the time of rats in the escape latency test is relatively short, indicating that the ratio of Chinese medicinal materials in the Zhuang medicine obtained by the method of the present invention is better for improving the cognitive impairment related to the hippocampus; In Example 1 of the Zhuang medicine obtained by the method of the present invention, compared with Comparative Example 5, the time of rats in the escape latency test is relatively short, indicating that the trial of the Zhuang medicine preparation method of the present invention is more conducive to the full analysis of the effective ingredients of Chinese medicinal materials, and the improvement effect of hippocampus-related cognitive impairment is better.

[0131] (2) Nissl staining of the hippocampal CA1 region of rats in each group: Three rats were randomly selected from each group for perfusion, and paraffin sections of the whole brain were obtained. The staining, washing, dehydration, transparency, and sealing were performed according to the instructions of the kit. The cell arrangement in the hippocampal CA1 region was observed under a light microscope and photographed (bar = 100 μm). The results are shown in Figure 1 , Figure 2 and Figure 3 Image-Pro Plus 6.0 was used to count neurons in the images, and the results are shown in Table 2.

[0132] Table 2 Comparison of the number of neurons in the hippocampal CA1 region of rats in each group

[0133] Group n Number of neurons (n) Sham operation group 3 66.67±7.77 Model Group 3 <![CDATA[22.33±4.93 * ]]> Example 1 3 <![CDATA[57.00±7.55 # <!-- 8 -->]]> Comparative Example 1 3 <![CDATA[51.33±7.58 # ]]> Comparative Example 2 3 <![CDATA[52.67±8.35 # ]]> Comparative Example 3 3 <![CDATA[51.33±7.83 # ]]> Comparative Example 4 3 <![CDATA[53.67±7.95 # ]]> Comparative Example 5 3 <![CDATA[52.33±6.85 # ]]>

[0134] The hippocampus is the main area of ​​the brain that helps humans process long-term learning and memory of events such as sound, light, and taste. It is responsible for the memory of major recent events in humans. The neurons in the CA1 region of the hippocampus have the function of sensing stimuli and transmitting excitement. They are the main cells that perform their functions in the hippocampus. Therefore, the number of neurons in the CA1 region of the hippocampus represents the strength of the short-term memory function of the hippocampus of the brain. As shown in Table 2, using Example 1 of the inventive method, compared with Comparative Examples 1-3, the number of neurons in the CA1 region of the hippocampus in the VD model rats is relatively large, indicating that the prescription using the inventive method has the effect of reducing neuronal damage and death; using Example 1 of the inventive method, compared with Comparative Example 4, the number of neurons in the CA1 region of the hippocampus in the VD model rats is more, indicating that the inventive method controls the ratio between the Chinese medicinal materials in the prescription, balances the dosage of each medicinal material, and reasonably cooperates with the drug power to reduce neuronal damage and death; using Example 1 of the inventive method, compared with Comparative Example 5, the number of neurons in the CA1 region of the hippocampus in the VD model rats is more, indicating that the inventive method performs a specific decoction method through the characteristics of the medicinal materials, so that the medicinal materials are fully analyzed while avoiding the volatilization of the effective ingredients, so that the prescription can play a curative effect to reduce neuronal damage and death. It is further explained that the prescription using the inventive method alleviates vascular dementia by reducing neuronal damage and death in the hippocampus.

[0135] The results are as follows Figure 1 , Figure 2 , Figure 3 As shown, the arrangement of cells in the CA1 region of the hippocampus observed under the light microscope of Example 1 and Comparative Examples 1-5 is more complete than that in the model group, and the cells are arranged neatly, indicating that the corresponding dose of the drug given to the VD model rat can maintain the integrity of the cells in the CA1 region of the hippocampus and reduce the damage and death of cells in the CA1 region of the hippocampus. In Example 1 using the method of the present invention, compared with Comparative Examples 1-5, the cells under the light microscope are arranged more closely to each other, and the number of intact cells is more, indicating that the Zhuang medicine obtained by the method of the present invention is more effective in reducing the damage of cells in the CA1 region of the hippocampus of the brain, and the integrity of the cells in the CA1 region of the hippocampus of the brain and the number of active cells that remain affect the strength of the short-term memory function of the hippocampus of the brain, so the method of the present invention can enhance the short-term memory function of the hippocampus of the brain, thereby alleviating vascular dementia.

[0136] (2) Detection of Notch1, Jagged1, VEGFA, and VEGFR2 protein expression in rat hippocampus: The remaining rat hippocampus was lysed and the total protein was extracted. The protein concentration was determined. Western blot gel electrophoresis was used to detect the expression of Notch1, Jagged1, VEGFA, and VEGFR2 proteins in the hippocampus of rats in each group. Each index was repeated 3 times. The image results were analyzed using image J software. The results are shown in Table 3.

[0137] Table 3 Comparison of Notch1, Jagged1, VEGFA and VEGFR2 protein expressions in the hippocampus of rats in each group

[0138]

[0139]

[0140] Note: * Indicates P < 0.05 compared with the normal group; # Indicates P < 0.05 compared with the model group.

[0141] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A Zhuang medicine for alleviating vascular dementia, characterized in that: The Zhuang medicine is made of main medicine, auxiliary medicine and supporting medicine: The raw materials of the main drug are 13-17g of Ganoderma lucidum, 13-17g of Angelica sinensis vine and 8-14g of Centipede arborea; The raw materials of the medicine are 8-14g of Radix Notoginseng, 13-17g of Radix Salviae Miltiorrhizae, 8-14g of Rhizoma Chuanxiong and 13-17g of Rhizoma Acorus Tatarinowii; The raw materials with medicine are 8-14g of Polygala tenuifolia and 8-14g of Arisaema ciliata.

2. The Zhuang medicine for alleviating vascular dementia according to claim 1, characterized in that: The raw materials of the main drug are 15g of Ganoderma lucidum, 15g of Angelica sinensis vine and 10g of Centipede arborealis in a weight ratio.

3. The Zhuang medicine for alleviating vascular dementia according to claim 1, characterized in that: The raw materials of the auxiliary medicine are 10g of Panax notoginseng, 15g of Salvia miltiorrhiza, 10g of Chuanxiong and 15g of Acorus calamus in a weight ratio.

4. The Zhuang medicine for alleviating vascular dementia according to claim 1, characterized in that: The raw materials with medicine are mixed in a weight ratio of 10g of Polygala tenuifolia and 10g of Arisaema ciliata.

5. The method for preparing the Zhuang medicine for alleviating vascular dementia according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Processing of medicinal materials: Weigh the medicinal materials according to the formula, slice the angelica vine and soak it in warm water, slice the arisaema ciliata and boil it with ginger juice over high heat for three times, then simmer it over low heat for several more times, boil it again, dry it in the air, and prepare the arisaema ciliata for later use; (2) First decoction: For the first decoction, boil the processed Arisaema confusa with Ganoderma lucidum, Scolopendra subspinipes and Salvia miltiorrhiza in water over high heat and then simmer for 40-47 minutes over low heat. Then add Panax notoginseng, Ligusticum chuanxiong, Acorus calamus and Polygala tenuifolia into the decoction and simmer for 25-30 minutes over low heat. Then pour the soaked Angelica sinensis vine slices and water into the first decoction and simmer for 10-15 minutes over high heat. Filter and pour out the first decoction to obtain the decoction. (3) Second decoction: Add an appropriate amount of water to the residue and place it in a decoction container for a second decoction. After the second decoction is simmered over high heat for 15-20 minutes, the liquid is poured out and filtered to obtain the second decoction liquid. The first decoction liquid and the second decoction liquid are mixed to obtain a Zhuang medicine decoction.

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