Weevil Rearing Method Based on an Egg Collector for Myllocerus aurolineatus Voss

Abstract

The present invention belongs to the technical field of insect breeding, and particularly relates to a method for breeding weevils based on an egg collector for Myllocerinus aurolineatus. The present invention includes the following steps: 1) capturing adults; 2) breeding adults; 3) collecting egg grains; 4) hatching egg grains; 5) planting tea seedlings; 6) breeding larvae; 7) observing and picking larvae; 8) collecting larvae; 9) obtaining pupae and adults. The present invention can specifically realize the indoor closed-chain breeding of Myllocerinus aurolineatus from adults - egg laying - hatching - larvae - pupae - adults, and can effectively ensure the indoor population quantity of Myllocerinus aurolineatus.

Description

[0001] This invention is a divisional application of the application with the application number CN201811120780.8 and the application name "Tea leaf weevil egg collector and weevil rearing method using this collector". The filing date of the original application is September 26, 2018. Technical Field

[0002] This invention belongs to the technical field of insect rearing, and particularly relates to a weevil rearing method based on a tea leaf weevil egg collector. Background Art

[0003] The tea leaf weevil, Myllocerinus aurolineatus Voss, also known as the tea weevil, belongs to the Coleoptera, Curculionidae, Myllocerinus Reitter, and is a major pest during the summer tea period in tea-growing areas of China. It is mainly distributed in provinces such as Zhejiang, Anhui, Jiangxi, Fujian, Hubei, Hunan, and Guangdong, and harms various plants such as tea, camellia oleifera, camellia, citrus, apple, pear, peach, and chestnut. In areas such as Zhejiang and Anhui, the tea leaf weevil has one generation per year in tea gardens. Generally, it pupates in late April, and adults begin to emerge from the soil in early and mid-May. The peak emergence of adults is from early June to early July, and the larvae overwinter in the tea garden soil. The adults chew the edges of young tea leaves into irregular notches, and when severe, only the main veins remain. The larvae can eat the fibrous roots in the soil, and when severe, the entire tea garden is bitten and incomplete. The tea soup made from the damaged tea leaves is turbid in color and the leaf bottom is broken.

[0004] Except for adults, all other life stages of the tea leaf weevil live in the soil, and adults are the main targets for control currently. Because the tea leaf weevil is a Coleoptera pest and adults have the habit of feigning death, it is difficult to control in the field. Currently, the control measures mainly use chemical measures. For severely damaged senescent tea gardens, heavy pruning or cutting back to the stump is required, and the branches and leaves are concentrated and burned for control, which will inevitably have a serious impact on the yield and quality of tea.

[0005] Currently, there is relatively little research on the artificial rearing technology of the tea leaf weevil in China, and the research level is relatively backward. In the process of using fresh tea branches and leaves to rear insects, the biggest problem is the preservation and replacement of the branches and leaves. Therefore, it is of great significance to extend the preservation time of the branches and leaves and save the replacement frequency. At the same time, after solving the problem of adult rearing, how to further realize the indoor closed-loop rearing of tea leaf weevils from adults - egg laying - egg hatching - larvae - pupae - adults based on the reared adults, and finally ensure the indoor population of tea leaf weevils through this indoor artificial rearing technology of tea leaf weevils, so as to effectively play the function of preventing and controlling tea leaf weevils, is also a technical problem that needs to be solved urgently in this field in recent years. Summary of the Invention

[0006] One of the objectives of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a weevil breeding method based on a tea leaf weevil egg collector, which can specifically realize the indoor closed-chain breeding of adult tea leaf weevils - egg laying - hatching - larvae - pupae - adults, and can effectively guarantee the indoor population quantity of tea leaf weevils, which is of great significance for the control research of tea leaf weevils.

[0007] To achieve the above objective, the present invention adopts the following technical solutions:

[0008] A weevil breeding method based on a tea leaf weevil egg collector, characterized in that: this collector includes a main barrel body in the shape of a straight circular barrel with an upward opening, and an intermediate partition that can perform an axial lifting action along the axis of the main barrel body is coaxially arranged in the barrel cavity of the main barrel body; the intermediate partition is densely provided with insertion holes for inserting the branch parts of fresh tea branches and leaves, so that after the intermediate partition divides the barrel cavity of the main barrel body into an upper barrel cavity and a lower barrel cavity, the upper barrel cavity constitutes a breeding cavity for breeding tea leaf weevils, and the lower barrel cavity constitutes a hydroponic accommodation cavity or a substrate accommodation cavity for hydroponically cultivating fresh tea branches and leaves or cutting fresh tea branches and leaves.

[0009] Preferably, the intermediate partition is driven by a lifting component to perform an axial lifting action along the main barrel body; the intermediate partition is in the shape of a circular plate that matches the shape of the barrel cavity of the main barrel body; the lifting component includes an axially adjustable telescopic rod, the bottom end of the telescopic rod is fixed to the upper plate surface of the intermediate partition and the rod body extends upward along the axis of the main barrel body, and a hook is arranged at the top end of the telescopic rod so as to be lapped and hooked on the barrel mouth of the main barrel body from top to bottom; the telescopic rod includes an upper threaded rod with a hook fixed to the top end of the rod body and a lower threaded rod with the bottom end of the rod body fixed to the intermediate partition, the adjacent ends of the upper threaded rod and the lower threaded rod are both provided with external threads and the thread directions of the external threads of the two are opposite to each other, and the telescopic rod further includes a threaded sleeve with internal threads, and the threaded sleeve forms a threaded fit with the upper threaded rod and the lower threaded rod respectively; the lifting component is two or more groups and is evenly distributed around the axis of the main barrel body.

[0010] Preferably, the intermediate partition is driven by a lifting component to perform an axial lifting action along the main barrel body; the intermediate partition is in the shape of a circular plate that matches the shape of the barrel cavity of the main barrel body; the lifting component includes an outer sleeve ring coaxially sleeved on the outer wall of the main barrel body, and a straight gear is arranged on the outer sleeve ring so as to form a gear-rack fit with a rack correspondingly arranged on the outer wall of the main barrel body; the straight gears are two or more groups and are evenly distributed along the circumference of the main barrel body; a ratchet wheel and a pawl for controlling the one-way rotation action of the ratchet wheel are coaxially fixed at each straight gear, so that the outer sleeve ring can only perform a downward sliding action relative to the outer wall of the main barrel body; a steering sheave is arranged at the barrel mouth of the main barrel body corresponding to each straight gear, one end of a traction rope is fixed to the upper ring surface of the outer sleeve ring, and the other end of the traction rope is fixed to the upper plate surface of the intermediate partition after being deflected by the steering sheave.

[0011] Preferably, hanging rings are arranged on the upper plate surface of the middle partition board corresponding to the number and positions of the steering grooved pulleys, and a fixing hook is arranged at one end of the towing rope for fixing the middle partition board. The fixing hook and the hanging ring are hooked with each other.

[0012] Preferably, the main barrel body includes a transparent straight pipe body and an end cover threadedly fixed at the bottom end of the straight pipe body. The inner thread is arranged in the bottom barrel cavity of the straight pipe body, and the external thread is arranged on the outer wall of the end cover whose outer shape is a barrel with an upward opening, so that the two form the above-mentioned threaded fixed connection; the barrel cavity of the end cover constitutes a hydroponic accommodation cavity or a substrate accommodation cavity for accommodating hydroponic liquid or cutting substrate.

[0013] The weevil breeding method based on the tea leaf weevil egg collector further includes the following steps:

[0014] 1) Capturing of adult weevils:

[0015] During the adult emergence period from the middle and late April to the middle and late June every year, the adult tea leaf weevils are captured in the tea garden by the method of patting and shaking them down.

[0016] 2) Breeding of adult weevils:

[0017] After picking the fresh tea branches and leaves, insert the fresh tea branches and leaves into the jacks on the middle partition board one by one, one branch per hole, and adjust the height of the middle partition board according to the length of the fresh tea branches and leaves, so that the bottom ends of the branches of the fresh tea branches and leaves are located in the cutting substrate or hydroponic liquid in the lower barrel cavity; then, transfer the captured adult weevils to the leaves of the above-mentioned fresh tea branches and leaves.

[0018] 3) Collection of egg grains:

[0019] Wrap the fresh tea branches and leaves in the upper barrel cavity with black cloth, so that the adult weevils lay eggs on the black cloth or on the branches and leaves of the fresh tea branches and leaves within the range wrapped by the black cloth.

[0020] 4) Hatching of egg grains:

[0021] Open the black cloth, pick and transfer the egg grains on the branches and leaves of the fresh tea branches and leaves onto the black cloth, then place the black cloth with the egg grains in a culture dish or beaker lined with filter paper, and place it in a constant temperature incubator at a temperature of 27°C ± 1°C and a relative humidity of 75% - 85% for hatching.

[0022] 5) Planting of tea seedlings:

[0023] Start planting tea seedlings in pots with tea seedling substrates at the beginning of the year.

[0024] 6) Breeding of larvae:

[0025] Transfer the newly hatched larvae in step 4) to the root systems of the established tea seedlings in step 5), inoculating 90 to 110 larvae per pot, and maintaining the breeding environment temperature at 25°C to 28°C;

[0026] 7), Observation and picking of larvae:

[0027] Start observing the growth and development of the larvae on the 15th day after step 6);

[0028] 8), Collection of larvae:

[0029] When collecting larvae for indoor experiments, pull out the tea seedlings from the pots and tap the root systems with a wooden stick to shake the larvae of Myllocerus aurolineatus and the attached soil on the root systems onto a black plastic bag, and then pick out the larvae with a medicine spoon; the substrate in the pots needs to be completely poured out and placed on the black plastic bag to search for and pick out the larvae; then re-plant the tea seedlings for later use;

[0030] 9), Obtaining of pupae and adults:

[0031] The remaining unused larvae continue to be in the pots and wait for them to pupate and emerge from the soil.

[0032] Preferably, in step 3), the distance between the bottom of the wrapped black cloth and the upper surface of the middle partition is 0.5 to 1.5 cm.

[0033] Preferably, in step 3), the black cloth needs to maintain a specified humidity, specifically, after soaking the black cloth, wring it until no water drips out by an adult male.

[0034] Preferably, in step 5), taking the total mass of the tea seedling substrate as ten parts, the tea seedling substrate ratio is: 4 parts of sandy loam + 4 parts of cinder ash + 2 parts of well-rotted organic fertilizer.

[0035] Preferably, in step 7), the way to observe the growth and development of the larvae is: use a wooden stick to pry open the tea seedling substrate until the root system of the tea seedling is seen, and then observe the growth of the surrounding larvae.

[0036] The main advantages of the present invention are:

[0037] 1), Through the above solution, the tea weevil egg collector of the present invention takes the main barrel body as the main structure, and uses an axially liftable intermediate partition to divide the barrel cavity of the main barrel body into an upper barrel cavity and a lower barrel cavity. On the one hand, the lower barrel cavity constitutes a hydroponic accommodation cavity or a substrate accommodation cavity, and utilizes the hydroponic or cutting effect on the inserted fresh tea branches and leaves, thereby realizing the long-term freshness preservation requirement of the fresh tea branches and leaves, which effectively reduces the workload of replacing adult food materials. At the same time, due to the reduction in the frequency of replacing fresh tea branches and leaves, the number of times the tea weevil is disturbed by the outside world is effectively reduced, the natural activities of the tea weevil are smoother, and its egg-laying amount and egg health can surely be effectively guaranteed. On the other hand, since the intermediate partition is a liftable partition, it is possible to adjust the height of the intermediate partition according to the length of the fresh tea branches and leaves, thereby effectively improving the utilization rate of small and short branches. For small and short branches, only need to adaptively adjust the intermediate partition to lower it to a suitable height; for large and long branches, vice versa, and its operation is extremely flexible. Finally, since the intermediate partition separates the breeding cavity from the hydroponic accommodation cavity or the substrate accommodation cavity, during the feeding process, the insect body will not directly fall into the water and drown due to accidents or its own feigning death characteristics, thereby effectively improving the breeding survival rate of adults.

[0038] 2), As a further preferred solution of the above solution, for the lifting action of the intermediate partition, it can actually be achieved in various ways, such as a controllable lifting motor or even an electric cylinder. The present invention preferably adopts the following two methods to achieve it: One is to use a bidirectional threaded sleeve rod, so as to utilize the threaded socket connection between the threaded sleeve and the upper threaded rod and the lower threaded rod, so that when the threaded sleeve is screwed, the upper threaded rod and the lower threaded rod can be driven to move towards and away from each other. Furthermore, when the hook is hooked and positioned at the barrel mouth of the main barrel body, the telescopic rod can achieve the purpose of controlling the lifting of the intermediate partition. The second is to adopt the method of an outer sleeve ring. On the one hand, the outer sleeve ring itself is sleeved on the outer wall of the main barrel body, and the rack-guided lifting action of the outer sleeve ring is realized through the gear-rack cooperation between the spur gear idling on the outer sleeve ring and the rack on the outer wall of the main barrel body. And the outer sleeve ring itself relies on the ratchet and pawl cooperation, so that the outer sleeve ring can only produce a downward movement in the free state, and it must specifically push aside the pawl to produce an upward movement. At the same time, the traction rope is fixed on the intermediate partition after being turned by the turning groove wheel. When the intermediate partition needs to be raised, only need to press down the outer sleeve ring so that the spur gear at the outer sleeve ring rolls relative to the rack. At this time, the traction rope is pulled to drive the intermediate partition to move upward. And when the intermediate partition needs to be lowered, push aside the pawl and press down the intermediate partition or even the fresh tea branches and leaves, and its use is extremely simple. The arrangement of the hanging ring and the fixed hook is to make the intermediate partition an independent part, so as to facilitate taking out the intermediate partition and cleaning it at any time, and even the plugging operation of the fresh tea branches and leaves relative to the intermediate partition can be carried out independently in the external environment to improve the plugging efficiency of the fresh tea branches and leaves into relatively small jacks.

[0039] 3) The main barrel body is formed by fitting a straight pipe body with an end cover, aiming to naturally form a hydroponic accommodation cavity or a substrate accommodation cavity in the barrel cavity of the end cover. When the nutrient in the hydroponic solution or the cutting substrate is insufficient, the end cover can be quickly disassembled through the threaded fit between the end cover and the main pipe body without disturbing the adults in the upper barrel cavity, and the hydroponic solution or the cutting substrate at the end cover can be quickly replaced in the external environment. Then, screw the end cover back to maintain the continuous online nutrient supply effect for the fresh tea branches and leaves, and finally ensure the freshness of the fresh tea branches and leaves.

[0040] 4) On the basis of the above collector structure, the present invention provides a method for raising Myllocerinus aurolineatus using the above collector. Using the raising method of the present invention, it can effectively overcome the problems that it is difficult to obtain the three insect states of eggs, larvae, and pupae of Myllocerinus aurolineatus indoors at present, and it is difficult to study other insect states except the adults of Myllocerinus aurolineatus, and can provide insect sources for relevant experimental studies on these three insect states indoors. The present invention can specifically realize the indoor closed-chain breeding of adults-eggs-larvae-pupae-adults of Myllocerinus aurolineatus, and can effectively guarantee the indoor population quantity of Myllocerinus aurolineatus, which has important significance for the prevention and control research of Myllocerinus aurolineatus.

[0041] 5) When wrapping the fresh tea branches and leaves with a black cloth as described above, the black cloth used must be a black cloth with a certain humidity, which is mainly set according to the specific habit of the adults laying eggs in the soil in the field and the requirements for the egg-laying environment, that is, artificially creating the most suitable egg-laying place. In addition, when wrapping the branches with the black cloth, a distance of 0.5-1 cm should be left between the black cloth and the middle partition of the collector, mainly according to the range of the body length of the adults of Myllocerinus aurolineatus being 6-7 mm, creating space for the egg-laying adults to move up and down, and increasing the air permeability inside the wrapped branches.

[0042] 6) In the above-mentioned matrix ratio for planting tea seedlings, the comprehensive requirements of tea seedling growth for nutrients and soil environment, and the growth of larvae for tea seedling roots and soil environment are effectively combined. Through repeated indoor experiments, the matrix ratio of 4 parts of sandy loam + 4 parts of furnace slag ash + 2 parts of decomposed organic fertilizer is finally the most suitable. This matrix has the fertility required for the growth and development of tea seedlings, and at the same time ensures the most suitable environment for the growth of Myllocerinus aurolineatus larvae, such as softness and air permeability. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 It is a structural cross-sectional view of one embodiment of the egg collector of Myllocerinus aurolineatus described in the present invention;

[0044] Figure 2 is Figure 1 the usage state diagram of the shown structure;

[0045] Figure 3Structural cross-sectional view of another embodiment of the tea leaf weevil egg collector described in the present invention;

[0046] Figure 4 Egg grain diagram of the tea leaf weevil;

[0047] Figure 5 Larva diagram of the tea leaf weevil.

[0048] Figures 1-3 The corresponding relationships between the reference numerals shown in the figure and the names of the components in the tea leaf weevil egg collector are as follows:

[0049] a - Fresh tea branches and leaves, b - Black cloth

[0050] 10 - Main barrel body, 10a - Rack, 11 - Straight pipe body, 12 - End cover

[0051] 20 - Intermediate partition, 21 - Insertion hole, 22 - Hanging ring

[0052] 31 - Upper threaded rod, 32 - Lower threaded rod, 33 - Threaded sleeve

[0053] 34 - Outer sleeve ring, 35 - Straight gear, 36 - Ratchet wheel, 37 - Pawl, 38 - Steering grooved wheel

[0054] 39 - Towing rope, 39a - Fixed hook, 40 - Hook Detailed implementation method

[0055] For the convenience of understanding, the specific structure and working mode of the present invention are further described below in conjunction with the accompanying drawings:

[0056] The tea leaf weevil egg collector described in the present invention has a main body structure including a main barrel body 10, a lifting assembly, and an intermediate partition 20, thus forming the collector structure as described. Among them: Figures 1-3 As described.

[0057] The main barrel body 10 serves as the main structure of the collector and undertakes the function of receiving the lifting assembly and the intermediate partition 20. During specific operation, the outer shape of the intermediate partition 20 is a circular plate shape adapted to the barrel cavity shape of the main barrel body 10, and the intermediate partition 20 is densely provided with insertion holes 21 similar to sieve holes. The height of the main barrel body 10 is 25 - 35 cm, and the diameter of the barrel cavity is 20 - 25 cm. The adjustable height range of the lifting assembly is usually 5 - 30 cm.

[0058] The aperture of the insertion hole 21 is 3 - 5 mm for inserting fresh tea branches and leaves a. This is designed according to the stem size of the fresh tea branches and leaves. On the basis of ensuring the utilization rate of branch cutting, it effectively reduces the death of the adult tea leaf weevil due to the large gap allowing it to penetrate into the lower substrate or water, and also reduces the problem that it is not conducive to the cutting of fresh branches due to the small gap.

[0059] Regarding the lifting assembly, there isFigures 1-2 The double-threaded sleeve rod shown, and Figure 3 the structure of the outer sleeve ring 34 shown. Among them:

[0060] As Figures 1-2 shown, when the lifting component is a double-threaded sleeve rod, it utilizes the threaded socket fit between the threaded sleeve 33 and the upper threaded rod 31 and the lower threaded rod 32. Thus, when the threaded sleeve 33 is turned, the upper threaded rod 31 and the lower threaded rod 32 can be driven to move towards each other and away from each other. Furthermore, when the hook 40 is hooked and positioned at the barrel opening of the main barrel body 10, the telescopic rod can achieve the purpose of lifting and controlling the middle partition 20.

[0061] As Figure 3 shown, when the lifting component is the outer sleeve ring 34 and is paired with the traction rope 39 and the steering sheave 38, on the one hand, the outer sleeve ring 34 itself is coaxially sleeved on the outer wall of the main barrel body 10, and through the gear-rack 10a cooperation mode between the spur gear 35 idling on the outer sleeve ring 34 and the rack 10a on the outer wall of the main barrel body 10, the gear-rack guided lifting action of the outer sleeve ring 34 is realized. And the outer sleeve ring 34 itself relies on the cooperation of the ratchet 36 and the pawl 37, so that the outer sleeve ring 34 can only produce a downward movement in the free state, and it must specifically push aside the pawl 37 to produce an upward movement, thereby limiting the movement direction of the outer sleeve ring 34. For details, refer to Figure 3 shown. At the same time, the traction rope 39 is fixed on the middle partition 20 after being turned by the steering sheave 38. When the middle partition 20 needs to be lifted, only need to press down the outer sleeve ring 34 so that the spur gear 35 on the outer sleeve ring 34 rolls relative to the rack 10a, and the traction rope 39 will be pulled to drive the middle partition 20 to move upward. And when the middle partition 20 needs to move downward, push aside the pawl 37 to release the directional constraint on the ratchet 36, and then press down the middle partition 20 or even the fresh tea branches and leaves a. Its use is extremely simple. The arrangement of the hanging ring 22 and the fixed hook 39a is to make the middle partition 20 a self-contained part, so as to facilitate taking out the middle partition 20 and cleaning it at any time, and even the plugging operation of the fresh tea branches and leaves a relative to the middle partition 20 can be carried out independently in the external environment to improve the plugging efficiency of the fresh tea branches and leaves a relative to the relatively small insertion holes 21.

[0062] The main barrel body 10 is formed by the straight pipe body 11 cooperating with the end cover 12. The purpose is to make the barrel cavity of the end cover 12 naturally form a hydroponic accommodation cavity or a substrate accommodation cavity. When the hydroponic solution or the cutting substrate is insufficient in nutrients, the end cover 12 can be quickly disassembled by the threaded fit between the end cover 12 and the main pipe body without disturbing the adults in the upper barrel cavity, and the hydroponic solution or the cutting substrate at the end cover 12 can be quickly replaced in the external environment. Then, screw the end cover 12 back to maintain the continuous online nutrient supply effect on the fresh tea branches and leaves a, and finally ensure the freshness of the fresh tea branches and leaves a.

[0063] For the convenience of understanding, the specific structure and working process of the present invention are further elaborated below with the following embodiments:

[0064] Embodiment 1:

[0065] 1) Capturing of adult insects:

[0066] During the adult occurrence period from the middle and late April to the middle and late June, the adult Myllocerinus aurolineatus Voss are captured in the tea garden by the method of patting and shaking. This method of patting and shaking is based on making use of the adult's habit of feigning death. Once disturbed, the adults will naturally fall due to feigning death, thus effectively reducing the harm to the adults during the capturing process.

[0067] 2) Rearing of adult insects:

[0068] Transfer the captured adults to the fresh tea branches and leaves in the Myllocerinus aurolineatus Voss egg collector.

[0069] 3) Collection of egg grains:

[0070] On the basis of using the Myllocerinus aurolineatus Voss egg collector to keep the adult insects fresh, wrap the middle and lower parts of the fresh tea branches and leaves a above the middle partition 20 with a black cloth b. The bottom of the wrapped black cloth b needs to be spaced about 1 cm from the middle partition 20 to reserve space for the adults to move up and down. The adults lay eggs on the black cloth b or on the branches within the range wrapped by the black cloth b. Before using the black cloth b for wrapping, the black cloth b needs to be soaked and wrung until no water comes out, so as to obtain a black cloth b with a certain humidity.

[0071] 4) Place the egg grains as shown Figure 4 on the black cloth b (the egg grains on the branches and even the stems are gently picked onto the black cloth b with a writing brush) in a petri dish or beaker lined with filter paper. The black cloth b still needs to maintain a certain humidity. Then place the black cloth b together with the egg grains in a constant temperature incubator at a temperature of 27°C ± 1°C and a relative humidity of 75% - 85% for egg grain hatching operation.

[0072] 5) Planting of tea seedlings:

[0073] Start planting 1 - 2-year-old tea seedlings in plastic pots at the beginning of the year. The diameter of the pot is selected to be 16.5 cm - 18.3 cm, and two seedlings are planted in each pot. Taking the total mass of the tea seedling substrate as ten parts, the ratio of the tea seedling substrate is: 4 parts of sandy loam + 4 parts of furnace slag ash + 2 parts of well-rotted organic fertilizer. This tea seedling substrate is soft and breathable, which is beneficial to the development of the tea seedling roots and the growth of the tea seedlings.

[0074] 6) Rearing of larvae:

[0075] Place as shown Figure 5The newly hatched larvae shown are transferred to the roots of the colonized tea seedlings, and the number of inoculated larvae per pot is controlled between 90 and 110. During the transfer, the newly hatched larvae are small and soft, so the operation process needs to be gentle and slow. The temperature of the breeding environment is controlled between 25°C and 28°C.

[0076] 7) Observation and picking of larvae:

[0077] After 15 days, observe the growth and development of the larvae. Specifically: gently use a wooden stick to open the substrate until the roots of the tea seedlings are seen, and then observe the growth of the surrounding larvae.

[0078] 8) Collection of larvae:

[0079] When collecting larvae for indoor experiments, gently pull out the tea seedlings from the pots and use a wooden stick to pat the roots to shake off the larvae of Myllocerus splendens together with the soil attached to the roots onto a black plastic bag, and then pick out the larvae with a medicine spoon. The substrate in the pot needs to be completely poured out and placed on the black plastic bag to search for and pick out the larvae. Then, re-plant the tea seedlings for later use.

[0080] 9) Obtaining pupae and adults:

[0081] The remaining unused larvae continue to be in the pot until they pupate and emerge from the soil.

Claims

1. Method for rearing weevils based on an egg collector for Myllocerinus aurolineatus Voss, characterized in that: The egg collector for Myllocerinus aurolineatus Voss includes a main barrel body (10) in the shape of a straight circular barrel with an upward opening. An intermediate partition (20) that can move axially along the axis of the main barrel body (10) is coaxially arranged in the barrel cavity of the main barrel body (10). The intermediate partition is densely provided with insertion holes (21) for inserting the branch parts of fresh tea branches and leaves, so that after the intermediate partition (20) divides the barrel cavity of the main barrel body (10) into an upper barrel cavity and a lower barrel cavity, the upper barrel cavity forms a rearing cavity for breeding Myllocerinus aurolineatus Voss, and the lower barrel cavity forms a hydroponic accommodation cavity or a substrate accommodation cavity for hydroponically cultivating fresh tea branches and leaves or cutting fresh tea branches and leaves; The intermediate partition (20) is driven by a lifting component to generate a lifting movement along the axis of the main barrel body (10). The outer shape of the intermediate partition (20) is in the shape of a circular plate that matches the shape of the barrel cavity of the main barrel body (10). The lifting component includes an outer sleeve ring (34) coaxially sleeved on the outer wall of the main barrel body (10). A spur gear (35) is arranged on the outer sleeve ring (34) to form a gear-rack cooperation with a rack (10a) correspondingly arranged on the outer wall of the main barrel body (10). There are two or more groups of the spur gears (35) and they are evenly distributed along the circumferential direction of the main barrel body (10). A ratchet wheel (36) is coaxially fixed at each spur gear (35), and a pawl (37) for controlling the one-way rotation of the ratchet wheel (36) is provided, so that the outer sleeve ring (34) can only make a downward sliding movement relative to the outer wall of the main barrel body (10). Steering grooved wheels (38) are arranged at the barrel mouth of the main barrel body (10) corresponding to each spur gear (35). One end of a traction rope (39) is fixed to the upper ring surface of the outer sleeve ring (34), and the other end of the traction rope (39) is fixed to the upper plate surface of the intermediate partition (20) after being deflected by the steering grooved wheel (38); and includes the following steps: 1), Capturing of adults: During the adult emergence period from the middle and late April to the middle and late June every year, use the method of beating and shaking to capture the adults of Myllocerinus aurolineatus Voss in the tea garden; 2), Rearing of adults: After picking fresh tea branches and leaves, insert the fresh tea branches and leaves one by one into the insertion holes (21) on the intermediate partition (20), one branch per hole, and adjust the height of the intermediate partition (20) according to the length of the fresh tea branches and leaves, so that the bottom end of the branch of the fresh tea branches and leaves is located in the cutting substrate or hydroponic solution in the lower barrel cavity; then, transfer the captured adults to the leaves of the above fresh tea branches and leaves; 3), Collection of egg grains: Wrap the fresh tea branches and leaves in the upper barrel cavity with a black cloth, so that the adults lay eggs on the black cloth or on the branches of the fresh tea branches and leaves within the range wrapped by the black cloth; 4), Hatching of egg grains: Open the black cloth, pick and transfer the egg grains on the branches and leaves of the fresh tea branches and leaves onto the black cloth, and then place the black cloth with egg grains in a petri dish or beaker lined with filter paper and place it in a constant temperature incubator at a temperature of 27°C ± 1°C and a relative humidity of 75% - 85% for hatching; 5), Planting of tea seedlings: Start planting 1 - 2-year-old tea seedlings in pots with tea seedling substrates at the beginning of the year; 6), Rearing of larvae: Transfer the newly hatched larvae in step 4) to the roots of the tea seedlings planted in step 5), inoculating 90 to 110 larvae per pot, and maintaining the breeding environment temperature at 25°C to 28°C; 7), Observation and picking of larvae: Start observing the growth and development of the larvae on the 15th day after step 6); 8), Collection of larvae: When collecting larvae for indoor experiments, pull out the tea seedlings from the pots and tap the roots with a wooden stick to shake off the larvae of Myllocerus aurolineatus together with the soil attached to the roots onto a black plastic bag. Then, pick out the larvae with a plastic medicine spoon; all the substrates in the pots need to be poured out and placed on the black plastic bag to search for and pick out the larvae; then re-plant the tea seedlings for later use; 9), Obtaining pupae and adults: The remaining unused larvae continue to stay in the pots until they pupate and emerge from the soil.

2. The weevil breeding method based on the weevil egg collector according to claim 1, characterized in that: Hanging rings (22) are provided on the upper plate surface of the middle partition plate (20) corresponding to the number and position of the turning sheave wheels (38). A fixing hook (39a) is provided at one end of the traction rope (39) for fixing the middle partition plate (20), and the fixing hook (39a) and the hanging ring (22) are hooked to each other.

3. The weevil breeding method based on the weevil egg collector according to claim 1 or 2, characterized in that: The main barrel body (10) includes a transparent straight pipe body (11) and an end cover (12) threadedly fixed to the bottom end of the straight pipe body (11). The bottom barrel cavity of the straight pipe body (11) is provided with internal threads, and external threads are arranged on the outer wall of the end cover (12) shaped like a barrel with an upward opening, so that the two form the above-mentioned threaded fixed connection; the barrel cavity of the end cover (12) constitutes a hydroponic accommodation cavity or a substrate accommodation cavity for accommodating hydroponic solution or cutting substrate.

4. The weevil breeding method based on the weevil egg collector according to claim 1, characterized in that: In step 3), the distance between the bottom of the wrapped black cloth and the upper plate surface of the middle partition plate (20) is 0.5 to 1.5 cm.

5. The weevil breeding method based on the weevil egg collector according to claim 1, characterized in that: In step 3), the black cloth needs to maintain a specified humidity, specifically, after completely soaking the black cloth, wring the black cloth by an adult male until no water drips.

6. The weevil breeding method based on the weevil egg collector according to claim 1, characterized in that: In step 5), taking the total mass of the tea seedling substrate as ten parts, the tea seedling substrate ratio is: 4 parts of sandy loam + 4 parts of furnace slag ash + 2 parts of well-rotted organic fertilizer.

7. The weevil breeding method based on the weevil egg collector according to claim 1, characterized in that: In step 7), the way to observe the growth and development of the larvae is: use a wooden stick to pry open the tea seedling substrate until the roots of the tea seedlings are seen, and then observe the growth of the surrounding larvae.

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