Use of lipopeptides for improving plant quality

Abstract

The present invention relates to the use of a composition comprising a lipopeptide for improving the quality of a plant. In particular, the present invention relates to the application of a composition comprising a lipopeptide to the plant as a seed coating, seed soaking, drenching or foliar spray. The plants treated with the composition have a significantly increased chlorophyll content, vitamin content, protein content, soluble sugar and / or anthocyanin content of the plants and / or their fruits, a shortened growth cycle, and / or an enhanced ability to resist drought, cold and / or lodging, as compared to a control group. Furthermore, the application of the composition further promotes the growth and yield of the plants while improving the quality of the plants.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biotechnology and bioengineering, in particular to the use of lipopeptides for improving plant quality. BACKGROUND

[0002] Lipopeptides are a kind of natural synthetic cyclic compounds composed of hydrophobic alkyl chain and hydrophilic polypeptide. Lipopeptides have antibacterial, antifungal, anti-inflammatory activities, and have the advantages of high efficiency, wide selectivity, biodegradability, etc. According to the type, number and ring-forming group of amino acids in the peptide ring, Bacillus lipopeptides can be divided into surfactin, fenjingsu, iturin, and orsellinic acid, etc. Among them, surfactin is a cyclic lipopeptide biosurfactant with excellent surface / interface activity, which can significantly reduce the surface tension of water, and show good antifungal, antiviral, antitumor, insecticidal and anti-mycoplasma activities, etc. It has great application potential in the fields of medicine, agriculture, food, daily chemical industry, and oil exploitation.

[0003] In the field of agriculture and forestry, lipopeptides can degrade residual pesticides. Adding lipopeptide biosurfactants or surfactant-producing bacteria to pesticide-contaminated soil can significantly degrade harmful ingredients. The degradation rate of endosulfan insecticide can be increased by 30%-45%. Lipopeptides have obvious inhibitory effect on plant pathogenic fungi, and have application value in plant disease control, which can be used for preventing and controlling plant diseases and pests and improving crop yield. Li Mingtong et al. reported that lipopeptides had obvious inhibitory effect on ginger root rot, and the incidence of ginger root rot decreased by 40.0%-56.7% and the disease index decreased by 41.0-58.9 after applying lipopeptides. Lipopeptides have obvious inhibitory effect on Fusarium graminearum, Fusarium oxysporum, Magnaporthe oryzae, and Erysiphe necator. Lipopeptides have good application prospect in biological control, food safety protection and soil pollution reduction. The popularization and application of lipopeptide biological pesticides will contribute to the sustainable development of green agriculture and organic agriculture, and provide new biological pesticide varieties for selection, which will bring social and economic benefits.

[0004] Flowers have the advantages of high quality, cleanliness, humidity regulation, air purification, etc., and have been popular at home and abroad. Vegetables contain various minerals, vitamins and dietary fiber, which play an important role in human physiological activities, and many nutrients needed by the human body come from the intake of vegetables.

[0005] Fruits have the characteristics of low energy, high content of vitamins, minerals, dietary fiber and plant compounds, and play an important role in maintaining human health, maintaining normal function of human intestinal tract and reducing the risk of chronic diseases. Fruits also contain various organic acids, aromatic substances and pigments, etc., which can stimulate appetite and aid digestion. Human life is closely related to green plants. At present, in plant cultivation management, plant growth is often regulated by applying nutrient solution, plant growth regulator, nitrogen fertilizer and the like.

[0006] In the above measures, the nutrient concentration of the nutrient solution is easily affected by root exudates, pH, water quality and other factors, and the effect is unstable. In addition, if the nutrient solution is applied to the soil, it is easily absorbed by the soil, and the nutrients available for plant root absorption are few and uneven. The commonly used nutrient solution contains various compounds, which are not easy to degrade and pollute the environment.

[0007] Most plant growth regulators have the function of plant hormones or belong to artificially synthesized plant hormones. Plant growth regulators must be used during the peak period of plant growth. If the use time is not appropriate, the plant growth will be inhibited.

[0008] Nitrogen fertilizer is needed throughout the entire growth period of plants and is the most demanded nutrient. Its main function is to promote the growth of stems and leaves, and it is suitable for base fertilizer and topdressing. It is extremely difficult to control the application amount of nitrogen fertilizer. If the application amount of nitrogen fertilizer is small, the plant growth will be slow, and the lower leaves will turn yellow. If there is an excess of nitrogen, the leaves will be too large, the internodes will be too long, the reproductive growth and the vegetative growth will be unbalanced, the plant will be overgrown and late in turning green, and it will be easy to drop flowers and fruits, and the fruit development will be slow.

[0009] In order to solve the various problems related to low quality in the current plant growth process, it is urgent to develop a method that can improve the quality of plants and / or promote plant growth, while minimizing chemical and biological pollution, and has no harm for long-term use.

[0010] [Reference]

[0011] CN202211352992

[0012] CN202211478828

[0013] CN202211098497

[0014] CN202211479391 SUMMARY

[0015] Specifically, the present application solves the technical problems existing in the prior art by the following technical solutions.

[0016] 1. A composition for improving the quality of plants, comprising:

[0017] (i) lipopeptides in the range of 0.001–100 g / L; and

[0018] (ii) Additives.

[0019] 2. The composition according to claim 1, wherein the lipopeptide is a cyclic lipopeptide or a linear lipopeptide, preferably the cyclic lipopeptide has one of the following formulas (I)-(III):

[0020] (I)

[0021] (II)

[0022] (III)

[0023] Where R represents the carbon chain of the fatty acid chain, A1, A2, A3…A m These represent the 1st, 2nd, 3rd...mth amino acids on the peptide chain, respectively. The carboxyl group of the fatty acid is attached to the N-terminus of A1. m The C-terminal carboxyl group is linked to the hydroxyl or amino groups of other amino acids in the fatty acid or peptide chain to form a cyclic structure;

[0024] The linear lipopeptide is the linear product obtained by opening the cyclic lipopeptide.

[0025] 3. The composition according to claim 1 or 2, wherein the lipopeptide is one or more selected from the group consisting of: surfactant family, iturin family, and fengycin family; preferably, the lipopeptide is one or more selected from the group consisting of: surfactant, iturin, fengycin, lichenysin, bamylocin, pumilacidin, bacillomycin, mycosubtilin, plipastatin, fusaricidin, kurstakin, paenibacterin, polymyxin, octapeptin, bacaucin, and polypeptin.

[0026] 4. The composition according to any one of items 1-3, wherein the lipopeptide is produced by bacteria, actinomycetes, fungi, or cyanobacteria, preferably the bacteria are wild-type or genetically engineered, more preferably the bacteria are selected from the group consisting of Bacillus spp. Bacillus ), Pseudomonas spp.Pseudomonas Streptomyces ( Streptomyces Arthrobacter spp. Arthrobacter ) and Burkholderia ( Burkholderia More preferably, the actinomycetes are selected from the group consisting of Streptomyces (Streptomyces genus). Streptomycetaceae ), *Acidobacterium* genus ( Amycolatopsis ) and Microbacteria ( Microbacterium More preferably, the fungus is selected from the group consisting of Aspergillus ( ). Aspergillus ), Microcystis ( Microascus ), genus *Pseudomonas* Stachylidium ), genus *Rust moniliforme* Myrothecium ), genus *Cladosporium* Sarocladium ), Micrococcus spp. Ophiosphaerella ) and the genus *Cyclocarya* ( Clavariopsis More preferably, the cyanobacteria are selected from the group consisting of marine cyanobacteria ( ). Okeania ), Litmus genus ( Cladonia ) and Penicillium genus ( Moorea More preferably, the lipopeptide is obtained by culturing Bacillus or Pseudomonas, more preferably, the lipopeptide is obtained by culturing Bacillus and regulating the expression of lipopeptide synthesis-related genes, and more preferably, the lipopeptide synthesis-related genes are selected from the following group: lipopeptide synthesis genes, transmembrane transport protein gene ycxA, biotin carboxylase gene yngH, spore synthesis genes spoIVA / B / C / F, spoVA / B / D / E and leucine synthesis pathway genes leuABCD / ilvK.

[0027] 5. The composition according to claim 4, wherein the lipopeptide synthesis gene is selected from the group consisting of: surfactant synthesis gene srfA, fen mustard synthesis gene fen, lichenin synthesis gene lic, bam mycotoxin synthesis gene bam, antifungal subtilisin myc, iturin synthesis gene itu, phosphatidylin synthesis gene pps, fusarium oxysporin fuscule, krs, polymyxin pmx, and octapeptidemycin oct.

[0028] 6. The composition according to any one of claims 1-5, further comprising 0.01-99.99% by weight of one or more components selected from the group consisting of: polysaccharides, oligosaccharides, dextrins, amino acids, oligopeptides, lipids, proteins, and fatty acids or derivatives thereof, inorganic salts, dispersants, wetting agents, and polymers.

[0029] 7. The composition according to any one of items 1-6, wherein the composition is a fermentation broth containing lipopeptides, an aqueous solution, a concentrate, or a partially purified and dried powder.

[0030] 8. The composition according to any one of items 1-7, wherein the composition is a culture medium obtained by culturing bacteria, actinomycetes, fungi and cyanobacteria capable of producing lipopeptides.

[0031] 9. The composition according to any one of items 1-8, wherein the plant is a non-leguminous plant, preferably selected from the group consisting of: grain or livestock plants, vegetable plants, fruit plants, horticultural plants, and flowering plants; more preferably, the grain or livestock plant is a grass; more preferably, the vegetable plant is selected from the group consisting of: solanaceae, cruciferous plants, chenopodiaceae, asteraceae, umbelliferae, cucurbitaceae, lamiaceae, malvaceae, and convolvulaceae; and more preferably, the fruit plant is selected from the group consisting of: strawberry, blueberry, and cranberry. The following are preferred horticultural plants: cranberries, cherries, peaches, plums, dates, apples, pears, citrus fruits, oranges, grapefruits, and bananas. More preferably, the horticultural plants are selected from the group consisting of: money trees, spider plants, pothos, wandering jenny, money trees, lucky bamboo, money trees, banyan trees, evergreen plants, and dwarf coconut palms. More preferably, the flowering plants are selected from the group consisting of: daffodils, dahlias, freesias, irises, peonies, peony, tulips, hyacinths, lilies, cyclamen, chrysanthemums, hostas, bellflowers, lilies of the valley, asters, daylilies, orchids, roses, Chinese roses, carnations, chrysanthemums, wild roses, and bellflowers.

[0032] 10. The composition according to claim 9, wherein the vegetable plant is selected from the group consisting of: eggplant, potato, tomato, pepper, lettuce, bok choy, Chinese cabbage, radish, rapeseed, spinach, celery, cucumber, loofah, bitter melon, pumpkin, winter melon, watermelon, cantaloupe, honeydew melon, perilla, catnip, mint, and okra.

[0033] 11. The composition according to claim 9, wherein the grain or livestock plant is selected from the group consisting of: wheat, rice, upland rice, corn, oats, rye, barley, buckwheat, millet, sorghum, highland barley, wild rice, taro, Job's tears, sugarcane, sugar beet, sweet potato, oat grass, goosegrass, ice grass, sand grass, sheep grass, crested wheatgrass, barley grass, new wheatgrass, romaine lettuce, bermudagrass, three-awn grass, sedge, duckbill grass, wild grass, double flower grass, blue stem grass, bamboo, sorghum, bullwhip, alfalfa and hollow axis grass.

[0034] 12. A method for preparing the composition according to any one of items 1-11, comprising:

[0035] Bacteria capable of producing lipopeptides are cultured under conditions suitable for lipopeptide production to obtain a culture medium containing 0.001-100 g / L lipopeptides, and this culture medium is used as the composition; or

[0036] The purified or partially purified lipopeptides are formulated into a solution or solid mixture containing 0.001-100 g / L of lipopeptides, and the solution or solid mixture is used as the composition.

[0037] 13. The method according to claim 12, further comprising: adding 0.01-99.99% by weight of one or more components selected from the group consisting of: polysaccharides, oligosaccharides, dextrins, amino acids, oligopeptides, proteins, lipids, and fatty acids or derivatives thereof, inorganic salts, dispersants, wetting agents, polymers, and warning color reagents to the culture medium, solution, or solid mixture, and using the resulting product as the composition.

[0038] 14. The method according to item 12 or 13, further comprising:

[0039] The culture medium, solution, solid mixture or product is concentrated and / or dried and / or partially purified and / or purified, and the concentrated and / or dried and / or partially purified and / or purified product is used as the composition.

[0040] 15. A method for improving plant quality using the composition described in any one of items 1-11, comprising:

[0041] (1) Add the composition to the culture of the plant;

[0042] (2) Cultivate and harvest the plant in the culture of (1);

[0043] (3) Optionally measure the quality parameters of the cultured plants and / or their fruits and compare them with the quality parameters of plants and / or their fruits cultured in a culture that does not contain the said lipopeptides, preferably the quality parameters being selected from the group consisting of: chlorophyll content, total nitrogen content, vitamin content, protein content, soluble sugar content, growth cycle, fatty acid content, amino acid content, anthocyanin content, lycopene content, carotenoid content, pectin content, drought resistance, cold resistance and / or lodging resistance.

[0044] 16. The method according to item 15, wherein the improvement in plant quality is characterized by the following:

[0045] The quality parameters of plants cultured with the addition of the composition are at least 110%, at least 120%, at least 130%, at least 140%, or at least 150% of those of the control group without the addition of the composition.

[0046] 17. The method according to item 15 or 16, wherein the growth of the plant is simultaneously promoted, preferably the promotion of growth is characterized by one or more of the following: germination rate, number of germinations, plant height, number of leaves, leaf width, root length, total length, number of flowers (branches), height of flower branches, thickness of flower stems, number of fruits, fruit shape, root weight, leaf weight, dry matter weight, number of tillers, weight per plant, weight per fruit, and plant yield.

[0047] 18. The method according to item 17, wherein the promotion of plant growth is characterized by the following:

[0048] The growth parameters of plants cultured with the addition of the composition are at least 110%, at least 120%, at least 130%, at least 140%, or at least 150% of those of the control group without the addition of the composition.

[0049] 19. Use of the composition according to any one of items 1-11 for improving plant quality.

[0050] 20. Use of the composition according to any one of items 1-11 for improving plant quality while promoting root and plant growth.

[0051] 21. Use of the composition according to any one of items 1-11 in agricultural fertilizers, agricultural adjuvants or pesticides.

[0052] To make the technical solution of the present invention clearer, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Attached Figure Description

[0053] Figure 1 The image shows the effect of the lipopeptide composition on improving the quality of maize. The upper left graph shows a comparison of the number of rows and kernels per row of maize harvested in the field between the control group and the lipopeptide treatment group. The upper right, lower left, and lower right graphs show that the soluble sugar content (%), anthocyanin content (mg / 100g), and fatty acid content (%) of maize in the lipopeptide treatment group were significantly higher than those in the control group.

[0054] Figure 2 The figure shows the effect of the lipopeptide composition on improving the quality of wheat. The left and right figures show that the soluble sugar content (%) and protein content (%) of wheat in the lipopeptide treatment group were significantly increased compared with the control group.

[0055] Figure 3 The image shows the effect of the lipopeptide composition on improving the quality of spinach. The left, middle, and right images show that the lipopeptide-treated spinach exhibited significantly higher levels of chlorophyll SPAD, total nitrogen (mg / g), and vitamins (mg / 100g) compared to the control group.

[0056] Figure 4 The figure shows the effect of the lipopeptide composition on improving the quality of cucumbers. The left, middle and right figures show that the soluble sugar content (%), protein content (%) and vitamin C content (mg / 100g) of cucumbers in the lipopeptide treatment group were significantly higher than those in the control group.

[0057] Figure 5The image shows the effect of the lipopeptide composition on improving the quality of winter melon. The left and right images show that the flowering and fruiting rates of the winter melon in the lipopeptide treatment group were significantly higher than those in the control group.

[0058] Figure 6 The figure shows the effect of the lipopeptide composition on improving the quality of strawberries. The figures show that the number of flowers, the number of fruits, the number of normal-shaped fruits, the soluble sugar content (g / 100g), the protein content (g / 100g), and the pectin content (g / 100g) of strawberries in the lipopeptide treatment group were all significantly higher than those in the control group.

[0059] Figure 7 The image shows a comparison of the growth of control group plants and lipopeptide treatment group plants in different plant groups. In small image 1, the left image shows a comparison of the above-ground growth of corn potted plants in the control group and the lipopeptide treatment group, and the right image shows a comparison of the root growth of corn potted plants in the control group and the lipopeptide treatment group. In small image 2, the left image shows corn seedlings in the control group and the lipopeptide treatment group, and the right image shows the morphology of corn after harvest in the control group and the lipopeptide treatment group. Small image 4 shows a comparison of the germination potential of wheat in the control group and the lipopeptide treatment group. Small image 5 shows a comparison of the root development of wheat in the control group and the lipopeptide treatment group. Small image 6 shows a comparison of wheat paper cultivation in the control group and the lipopeptide treatment group. Small image 7 shows a comparison of the growth of crested wheat in the control group and the lipopeptide treatment group. In small image 8, the upper and lower images show a comparison of the growth of oat grass roots and above-ground plants in the control group and the lipopeptide treatment group, respectively. Small images 9-11 and 13-17b show a comparison of the growth of spinach, bok choy, cucumber, money tree, pothos, dahlia, daffodil, and freesia in the control group and the lipopeptide treatment group, respectively. Invention Details

[0061] 1. Definition

[0062] The term "lipopeptide" used in this invention, also known as acylpeptide, generally refers to a peptide composed of fatty acids and peptide chains linked by ester or amide bonds. It can be classified into cyclic lipopeptides and linear lipopeptides. Cyclic lipopeptides are lipopeptides with a cyclic structure, where the carboxyl group of the fatty acid is linked to the N-terminus of an amino acid in the peptide chain, and the C-terminal carboxyl group of the amino acid in the peptide chain is linked to the hydroxyl or amino group of the fatty acid or other amino acids in the peptide chain to form a cyclic structure. The cyclic forms of cyclic lipopeptides include: fatty acid hydroxyl ring formation, fatty acid amino ring formation, and fatty acid cyclization or decyclization, each with the following formula:

[0063]

[0064] (I) Cyclic formation of fatty acid hydroxyl groups

[0065]

[0066] (II) Cyclic formation of fatty acid amino groups

[0067]

[0068] (III) Fatty acid cyclization (A) m (forming a ring with A1) or fatty acid cyclic (A) m (Cycling with amino acids other than A1)

[0069] Lipopeptides are mainly produced by bacteria, preferably by Bacillus spp. Bacillus ), Pseudomonas spp. Pseudomonas Streptomyces ( Streptomyces ) or Arthrobacter spp. ( Arthrobacter (See Janek et al., 2010). The three well-known lipopeptide families are the surfactantin family, the iturin family, and the fengycin family. Lipopeptides can be one or more selected from the following groups: surfactantin, iturin, fengycin, lichenin, babylin, permiratin, spore-forming mycotoxin, antifungal mycotoxin, phosphatidylin, fusarium oxysporin, kurstakicin, spore-forming mycotoxin, polymyxin, octapeptide, and polypeptide mycotoxin. The main differences between them lie in the length of the fatty acid side chain, the isomerism, and the types, number, and sequence of amino acids in the peptide ring.

[0070] The surfactant family comprises more than 20 different molecules, which are composed of C 12-16 The β-hydroxy fatty acid condenses with a 7-amino acid polypeptide via a lactone bond to form a cyclic peptide (Bonmatin et al., 2003). The heptapeptide moiety has an LLDLLDL chiral sequence, and the chirality of the D-Leu at positions 3 and 6 and the L-amino acid at position 4 is strictly conserved, playing a key role in peptide chain closure (Peypoux et al., 1999). Meanwhile, amino acid substitutions such as Leu, Val, Ile, and Ala can be observed at positions 2, 4, and 7 (Bonmatin et al., 1995; Peypoux et al., 1991; Peypoux et al., 1994). The molecular structure of surfactant is shown in formula (IV) below:

[0071]

[0072] (IV) Amino acid sequence and linkage of surfactants

[0073] The iturobrine family currently comprises six main members: iturobrine A and C, spore-forming mycotoxins D, F, and L, and antifungal mycotoxins (Bonmatin et al., 2003). They are all derived from C... 14-17 It is a cyclic peptide formed by the condensation of β-amino fatty acids and 7 amino acids via lactam bonds. All molecules in the iturobrine family strictly adhere to the chiral conformation of LDDLLDL and contain a common amino acid sequence: β-amino fatty acid -L-Asx-D-Tyr-D-Asn (Peypoux et al., 1978). The molecular structure of iturobrine is shown in formula (V) below:

[0074]

[0075] (V) Amino acid sequence and linkage of ituronic acid

[0076] The fenswabin family is the third largest lipopeptide family after the surfactant and ituronic acid families. Members of the fenswabin family are composed of C... 14-17 The β-hydroxy fatty acid is linked to the 1st Glu of the decapeptide cyclic peptide via an ester bond, and the lactone bond is formed by the hydroxyl group at the 3rd Tyr position and the carbon terminus at the 10th Ile position (Nishikiori et al., 1986). The molecular structure of fenbufen is shown in formula (VI) below:

[0077]

[0078] (VI) Amino acid sequence and linkage of fenbufen

[0079] The terms “gene,” “expression enhancement,” “promoter,” “recombinant (bacterium),” and “culture medium” used in this invention are all conventional terms used in the field of molecular biology and have the same meaning as understood by those skilled in the art.

[0080] 2. The lipopeptide of the present invention

[0081] The lipopeptides of the present invention are cyclic lipopeptides or linear lipopeptides. In one embodiment, the cyclic lipopeptides of the present invention have one of the following formulas (I)-(III):

[0082] (I)

[0083] (II)

[0084] (III)

[0085] Where R represents the carbon chain of the fatty acid chain, A1, A2, A3…A mThese represent the 1st, 2nd, 3rd...mth amino acids on the peptide chain, respectively. The carboxyl group of the fatty acid is attached to the N-terminus of A1. m The C-terminal carboxyl group is linked to a hydroxyl or amino group of a fatty acid or other amino acid in the peptide chain to form a cyclic structure. In one embodiment, the linear lipopeptide of the present invention is the linear product obtained by ring-opening the cyclic lipopeptide. In one embodiment, m in formulas (I)-(III) is an integer from 6 to 20. In one embodiment, the lipopeptide of the present invention is one or more selected from the group consisting of surfactants, iturobrine, and fenvalerate. In one embodiment, the lipopeptide of the present invention is selected from the group consisting of: surfactantin, iturin, fengycin, lichenysin, bamylocin, pumilacidin, bacillomycin, mycosubtilin, plipastatin, fusaricidin, kurstakin, paenibacterin, polymyxin, octapeptin, bacaucin, and polypeptin. In one embodiment, the lipopeptide of the present invention is a surfactant. In one embodiment, the lipopeptide of the present invention is iturin. In one embodiment, the lipopeptide of the present invention is fengycin.

[0086] In one embodiment, the lipopeptide of the present invention is produced by bacteria, actinomycetes, fungi, or cyanobacteria. In one embodiment, the lipopeptide of the present invention is produced by bacteria. In one embodiment, the bacteria are wild-type fungi or genetically engineered fungi. In one embodiment, the bacteria are selected from the group consisting of Bacillus spp. (…). Bacillus ), Pseudomonas spp. Pseudomonas Streptomyces ( Streptomyces Arthrobacter spp. Arthrobacter ) and Burkholderia spp. Burkholderia In one embodiment, the lipopeptide of the present invention is derived from Bacillus alkalophilus (…). Bacillus alkalophilus ), Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ), Bacillus brevis ( Bacillus brevis ), Bacillus circularis ( Bacillus circulans ), Bacillus clausti ( Bacillus clausii ), Bacillus coagulans ( Bacillus coagulans ), Bacillus stolonifer ( Bacillus firmus), Bacillus splendens ( Bacillus lautus ), Bacillus tarda ( Bacillus lentus ), Bacillus licheniformis ( Bacillus licheniformis ), Bacillus megaterium ( Bacillus megaterium ), Bacillus pumilus ( Bacillus pumilus ), thermophilic steatobacterium ( Bacillus stearothermophilus Bacillus subtilis ( Bacillus subtilis ) or Bacillus thuringiensis ( Bacillus thuringiensis Produced by Pseudomonas aeruginosa. In one embodiment, the lipopeptides of the present invention are produced by Pseudomonas aeruginosa. Pseudomonas aeruginosa ), Fluorescent Pseudomonas ( Pseudomonas fluorescens ), Pseudomonas melioides ( Pseudomonas mallei ) or melioidosis pseudomonas ( Pseudomonas pseudomallei Produced by ). In one embodiment, the lipopeptides of the present invention are produced by non-chromogenic Streptomyces ( Streptomyces achromogenes ), insecticide streptomycin ( Streptomyces avermitilis ), Streptomyces azureus ( Streptomyces coelicolor ), Streptomyces griseus ( Streptomyces griseus ) or Streptomyces purpureus ( Streptomyces lividans ) is produced. In one embodiment, the lipopeptides of the present invention are produced by *Arthrobacter* ( Arthrobacter ) or Cladosporium flavum ( Arthrobacter The lipopeptides of the present invention are produced by *Burkholderia cepacia*, *Burkholderia melioides*, or *Burkholderia melioides*. In one embodiment, the lipopeptides of the present invention are produced by *Streptomyces* spp. Streptomycetaceae ), *Acidobacterium* genus ( Amycolatopsis ) and Microbacteria ( Microbacterium ) is produced. In one embodiment, the lipopeptides of the present invention are produced by Aspergillus ( ). Aspergillus ), Microcystis ( Microascus ), genus *Pseudomonas* Stachylidium ), genus *Rust moniliforme* Myrothecium ), genus *Cladosporium* Sarocladium ), Micrococcus spp. Ophiosphaerella ) and the genus *Cyclocarya* ( Clavariopsis In one embodiment, the lipopeptides of the present invention are produced by marine cyanobacteria ( ). Okeania ), Litmus genus ( Cladonia ) and Penicillium genus ( Moorea (Produced by)

[0087] In one embodiment, the lipopeptide of the present invention is obtained by culturing Bacillus or Pseudomonas. In one embodiment, the lipopeptide of the present invention is obtained by culturing Bacillus and regulating the expression of lipopeptide synthesis-related genes. In one embodiment, the lipopeptide synthesis-related genes are selected from the group consisting of: lipopeptide synthesis genes, transmembrane transport protein gene ycxA, biotin carboxylase gene yngH, spore synthesis genes spoIVA / B / C / F, spoVA / B / D / E, and leucine synthesis pathway genes leuABCD / ilvK. In one embodiment, the lipopeptide synthesis genes are selected from the group consisting of: surfactant gene srfA, fenamic acid synthesis gene fen, lichenin synthesis gene lic, bamic acid synthesis gene bam, antifungal subtilisin myc, iturin synthetin synthesis gene itu, phosphatidylinosin synthesis gene pps, fusarium oxysporin fuscitate ... In one embodiment, the expression of genes regulating lipopeptide synthesis is selected from the group consisting of: overexpression of the srfA gene, overexpression of ycxA, overexpression of yngH, knockout of spoIVA / B / C / F, knockout of spoV A / B / D / E, overexpression of leuABCD, and overexpression of ilvK. In one embodiment, overexpression of the srfA gene is obtained by replacing the natural promoter PsrfA with the Pg3 promoter. In one embodiment, the Bacillus is Bacillus subtilis THY-7 / Pg3-srfA.

[0088] 3. The composition of the present invention for improving plant quality.

[0089] In one aspect, the present invention relates to a composition for improving the quality of plants, comprising 0.001-100 g / L of lipopeptides and adjuvants. In one embodiment, the composition contains 0.001 g / L, 0.002 g / L, 0.003 g / L, 0.004 g / L, 0.005 g / L, 0.006 g / L, 0.007 g / L, 0.008 g / L, 0.009 g / L, 0.01 g / L, 0.02 g / L, 0.03 g / L, 0.04 g / L, 0.05 g / L, 0.06 g / L, 0.07 g / L, 0.08 g / L, 0.09 g / L, 0.1 g / L, 0.2 g / L, 0.3 g / L, 0.4 g / L, 0.5 g / L, 0.6 g / L, 0.7 g / L, 0.8 g / L, 0.9 g / L, 1 g / L, 2 g / L, 3 g / L, 4 g / L, 5 g / L. Lipopeptides in the range of g / L, 6 g / L, 7 g / L, 8 g / L, 9 g / L, 10 g / L, 15 g / L, 20 g / L, 25 g / L, 30 g / L, 35 g / L, 40 g / L, 45 g / L, 50 g / L, 55 g / L, 60 g / L, 65 g / L, 70 g / L, 75 g / L, 80 g / L, 85 g / L, 90 g / L, 95 g / L, or 100 g / L (or combinations thereof). In one embodiment, the composition contains 0.001-0.005 g / L, 0.001-0.01 g / L, 0.001-0.03 g / L, 0.001-0.05 g / L, 0.001-0.1 g / L, 0.001-0.5 g / L, 0.001-0.8 g / L, 0.001-1.0 g / L, 0.001-1.5 g / L, 0.001-2.5 g / L, 0.001-5 g / L, 0.001-8 g / L, 0.001-12 g / L, 0.001-18 g / L, 0.001-25 g / L, 0.001-35 g / L, 0.001-45 g / L, 0.001-55 g / L, 0.001-65 g / L. The composition contains lipopeptides at concentrations of g / L, 0.001-75 g / L, 0.001-85 g / L, or 0.001-95 g / L. In one embodiment, the composition of the present invention further comprises 0.01-99.99% by weight of one or more components selected from the group consisting of: polysaccharides, oligosaccharides, dextrins, amino acids, oligopeptides, proteins, lipids, fatty acids or derivatives thereof, inorganic salts, dispersants, wetting agents, polymers, and warning color reagents. In one embodiment, the polysaccharide is selected from the group consisting of: peptidoglycans, cellulose, glycogen, starch, chitosan, fructooligosaccharides, galactoses, and glycosaminoglycans. In one embodiment, the amino acid is a common α-amino acid.In one embodiment, the amino acid is selected from the group consisting of: alanine, aspartic acid, arginine, glutamic acid, cysteine, glycine, serine, phenylalanine, histidine, isoleucine, lysine, leucine, methionine, asparagine, proline, glutamine, tryptophan, threonine, valine, and tyrosine. In one embodiment, the oligopeptide is a peptide consisting of 2-10 amino acids, a peptide consisting of 2-8 amino acids, or a peptide consisting of 2-6 amino acids. In one embodiment, the oligopeptide is selected from the group consisting of: oligopeptide-3, oligopeptide-4, oligopeptide-5, and oligopeptide-6. In one embodiment, the lipid is selected from the group consisting of: lipid compounds, phospholipids, glycolipids, and cholesterol and their esters. In one embodiment, the fatty acid is selected from the group consisting of: saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids. In one embodiment, the fatty acid or its derivative is selected from the group consisting of: stearic acid, animal fats and oils, and vegetable oils. In one embodiment, the inorganic salt is selected from the group consisting of: sulfates, hydrogen phosphates, dihydrogen phosphates, phosphates, nitrates, and superphosphates. In one embodiment, the inorganic salt is selected from the group consisting of sodium salts, potassium salts, calcium salts, iron salts, and ammonium salts. In one embodiment, the inorganic salt is selected from the group consisting of Na₂SO₄, K₂SO₄, (NH₄)₂SO₄, KH₂PO₄, K₂HPO₄, Na₂HPO₄, NaH₂PO₄, K₃PO₄, CO(NH₂)₂, NH₄NO₃, NaNO₃, CaCl₂, FeCl₂, NH₄Cl, MnSO₄, FeSO₄, Ca(H₂PO₄)₂, and their hydrates. In one embodiment, the dispersant is selected from the group consisting of naphthalene sulfonates, lignin sulfonates, polycarboxylates and polycarboxyl esters, starch, bovine serum albumin, and cyclodextrin. In one embodiment, the wetting agent is selected from the group consisting of dodecyl sulfonates, octyl succinates, and alcohol ether sulfates. In one embodiment, the polymer is selected from the group consisting of polyethylene glycol, polyacrylic acid emulsions, polyacrylic acid polyvinyl alcohol emulsions, xanthan gum, and polyglutamic acid. In one embodiment, the warning color reagent is selected from the group consisting of carmine or fruit green. In one embodiment, the composition of the present invention is a culture medium containing 0.001-100 g / L lipopeptides obtained by culturing bacteria capable of producing lipopeptides. In one embodiment, the composition of the present invention is a solution or solid mixture containing 0.001-100 g / L lipopeptides prepared from purified or partially purified lipopeptides. In one embodiment, the composition of the present invention is an aqueous solution, concentrate, or dry powder formulation containing lipopeptides.

[0090] In one embodiment, the plant described in this invention is a non-leguminous plant. In one embodiment, the plant is selected from the group consisting of: grain or livestock plants, vegetable plants, fruit plants, horticultural plants, and flowering plants. In one embodiment, the grain or livestock plant is a grass (Poaceae). In one embodiment, the grain or livestock plant is selected from the group consisting of: wheat, rice, upland rice, corn, oats, rye, barley, buckwheat, millet, sorghum, highland barley, wild rice, taro, Job's tears, sugarcane, sugar beet, sweet potato, oat grass, *Gnaphalium affine*, *Isodon japonicus*, *Phyllostachys edulis*, *Leymus chinensis*, *Leymus chinensis*, barley grass, new barley grass, *Rhizophora stylosa*, *Cynodon dactylon*, *Tripterygium wilfordii*, *Miscanthus sinensis*, *Duckbillella asiatica*, *Eriocaulon buergerianum*, *Lysimachia nummularia*, *Indigofera tinctoria*, *Isodon japonicus*, *Sorghum sibthorpioides*, *Alfalfa*, and *Imperata cylindrica*. In one embodiment, the vegetable plants are selected from the group consisting of: Solanaceae, Brassicaceae, Chenopodiaceae, Asteraceae, Apiaceae, Cucurbitaceae, Lamiaceae, Malvaceae, and Convolvulaceae. In one embodiment, the vegetable plants are selected from the group consisting of: eggplant, potato, tomato, pepper, lettuce, bok choy, Chinese cabbage, radish, rapeseed, spinach, celery, cucumber, loofah, bitter melon, pumpkin, winter melon, watermelon, cantaloupe, honeydew melon, perilla, catnip, mint, and okra. In one embodiment, the fruit plants are selected from the group consisting of: strawberry, blueberry, raspberry, cranberry, cherry, peach, plum, jujube, apple, pear, citrus, orange, grapefruit, and banana. In one embodiment, the horticultural plants are selected from the group consisting of: money tree, spider plant, pothos, wandering jenny, money tree, lucky bamboo, money tree, banyan tree, evergreen, and dwarf palm. In one embodiment, the flowering plants are selected from the group consisting of: daffodils, dahlias, freesias, irises, peonies, peony, tulips, hyacinths, lilies, cyclamen, chrysanthemums, hostas, bellflowers, lilies of the valley, asters, daylilies, orchids, roses, Chinese roses, carnations, chrysanthemums, wild roses, and bellflowers.

[0091] 4. Method for preparing the composition of the present invention

[0092] In one aspect, the present invention relates to a method for preparing a composition for improving the quality of plants. In one embodiment, the method comprises: culturing lipopeptide-producing bacteria under conditions suitable for lipopeptide production to obtain a culture medium containing 0.001-100 g / L of lipopeptides, and using said culture medium as the composition of the present invention; or preparing purified or partially purified lipopeptides into a solution or solid mixture containing 0.001-100 g / L of lipopeptides, and using said solution or solid mixture as the composition of the present invention. In one embodiment, the solution is an aqueous solution. In one embodiment, the solvent in the aqueous solution is deionized water or tap water. In one embodiment, the method further comprises: adding 0.01-99.99% by weight of one or more components selected from the group consisting of: polysaccharides, oligosaccharides, dextrins, amino acids, oligopeptides, proteins, lipids, fatty acids or derivatives thereof, inorganic salts, dispersants, wetting agents, polymers, and warning color reagents to the culture medium, solution, or solid mixture, and using the resulting product as the composition of the present invention. In one embodiment, the method further includes: concentrating and / or drying and / or partially purifying the culture medium, solution, solid mixture or product, and using the concentrated and / or dried and / or partially purified product as the composition of the present invention.

[0093] 5. The method of the present invention for improving plant quality

[0094] In one aspect, the present invention relates to a method for improving the quality of plants using the compositions of the present invention. In one embodiment, the method comprises: (1) adding the compositions of the present invention to a culture of the plant (including watering the composition into the soil in which the plant is grown, spraying it onto the plant, or placing the plant in the composition (coating, soaking, or root drenching)); (2) culturing and harvesting the plant; and (3) optionally measuring quality parameters of the cultured plant and / or its fruit and comparing them with quality parameters of the plant and / or its fruit cultured in a culture (e.g., soil) that does not contain the lipopeptides. In one embodiment, the quality parameters are selected from the group consisting of: chlorophyll content, total nitrogen content, vitamin content, protein content, soluble sugar content, fatty acid content, amino acid content, anthocyanin content, lycopene content, carotenoid content, pectin content, growth cycle, drought resistance, cold resistance, and / or lodging resistance. In one embodiment, the improvement in plant quality is characterized by one or more of the following: a quality parameter of the plant cultured with the addition of the composition that is at least 110%, at least 120%, at least 130%, at least 140%, at least 150%, at least 160%, at least 170%, at least 180%, at least 190%, at least 200%, at least 210%, at least 220%, at least 230%, at least 230%, at least 250%, at least 260%, at least 270%, at least 280%, at least 290%, at least 300%, at least 350%, at least 400%, at least 450%, at least 500%, at least 550%, at least 600%, at least 650%, at least 700%, at least 750%, at least 800%, at least 850%, at least 900%, at least 950%, or at least 1000% of the control group without the addition of the composition.

[0095] In one aspect, the present invention relates to a method for improving the quality of plants and promoting plant growth using the compositions of the invention. In one embodiment, the promotion of plant growth is characterized by one or more of the following: germination rate, number of buds, plant height, number of leaves (number of new leaves), leaf width, root length, total length, number of flowers (branches), height of flowering branches, thickness of flowering stems, number of fruits, fruit shape, root weight, leaf weight, dry matter weight, number of tillers, weight per plant, weight per fruit, and plant yield.

[0096] In one embodiment, the promotion of plant growth is characterized by one or more of the following: growth parameters of plants cultured with the addition of the composition that are at least 110%, at least 120%, at least 130%, at least 140%, at least 150%, at least 160%, at least 170%, at least 180%, at least 190%, at least 200%, at least 210%, at least 220%, at least 230%, at least 230%, at least 250%, at least 260%, at least 270%, at least 280%, at least 290%, at least 300%, at least 350%, at least 400%, at least 450%, at least 500%, at least 550%, at least 600%, at least 650%, at least 700%, at least 750%, at least 800%, at least 850%, at least 900%, at least 950%, or at least 1000% of the control group without the addition of the composition.

[0097] In one embodiment, the composition of the present invention is added in a manner selected from the group consisting of: seed coating, seed soaking, bulb treatment, root irrigation, drip irrigation, and foliar spraying.

[0098] 6. Uses of the compositions of the present invention

[0099] In one aspect, the present invention relates to the use of the compositions of the present invention for improving plant quality. In another aspect, the present invention relates to the use of the compositions of the present invention for improving plant quality while promoting plant and / or root growth. In one embodiment, the compositions of the present invention are used in agricultural fertilizers, agricultural adjuvants, or pesticides. In one embodiment, the fertilizer is an amino acid-containing water-soluble fertilizer, an organic water-soluble fertilizer, a bio-organic fertilizer, a chemical fertilizer, a microbial fertilizer, a microbial concentrate, a humic acid fertilizer, a soil conditioner, a soil remediation agent, a slow-release fertilizer, a plant growth stimulant, or an inoculant. In one embodiment, the agricultural adjuvant is an insecticide adjuvant, a fungicide adjuvant, a fertilizer adjuvant, an acaricide adjuvant, a rodenticide adjuvant, a nematicide adjuvant, or an agricultural synergist. In one embodiment, the pesticide is a plant growth regulator, an insecticide, a fungicide, a herbicide, or a rodenticide.

[0100] 7. Advantages of the present invention

[0101] This invention has the following technical advantages:

[0102] (1) The composition of the present invention can significantly improve plant quality. Compared with the control, the plants applied with the lipopeptide composition have higher chlorophyll content, total nitrogen content, vitamin content, protein content, soluble sugar content, fatty acid content, amino acid content, anthocyanin content, lycopene content, carotenoid content and / or pectin content, shorter growth cycle, and / or stronger drought resistance, cold resistance and / or lodging resistance.

[0103] (2) The composition of the present invention has a significant effect on improving plant quality and promoting plant growth. Compared with the control, the plants applied with the lipopeptide composition have higher seedling rate, number of germinations, plant height, number of leaves, leaf width, root length, total length, number of flowers (branches), height of flower branches, thickness of flower stems, number of fruits, root weight, leaf weight, dry matter weight, number of tillers, weight per plant, weight per fruit and / or plant yield, better leaf color and fruit shape, and / or longer flowering period.

[0104] (3) The lipopeptides used in the compositions of the present invention are biosurfactants, which are biodegradable, pollution-free, environmentally friendly, and harmless with long-term use; and

[0105] (4) The lipopeptides used in the composition of the present invention are highly stable and less affected by temperature and humidity. The preparation and use methods are simple and easy to manage. Detailed Implementation

[0106] The present invention will be further described below with reference to specific embodiments, but these specific embodiments should not be construed as limiting the scope of protection of the present invention. Those skilled in the art can make various changes or modifications to these specific embodiments without departing from the scope of the technical solution of the present invention, and the changed and modified implementation schemes still fall within the protection scope of the present invention.

[0107] Instruments, materials and reagents

[0108] The clean bench, shaker, incubator, shake flask, genetically engineered bacteria THY-7 (pJMP-yngH), sugars, inorganic nitrogen source, organic nitrogen source, KH2PO4, Na2HPO4·12H2O, CaCl2, MnSO4·H2O, FeSO4·7H2O, etc. are all commercially purchased products.

[0109] strain

[0110] Bacillus subtilis ( Bacillus subtilisTHY-7 / Pg3-srfA is derived from patent ZL201510654218.3; Bacillus subtilis THY-7 / Pg3-srfA(yngH) is derived from patent ZL 2018108652957; Bacillus subtilis THY-7 / Pg3-srfAΔspoIVA, THY-7 / Pg3-srfAΔspoIVB, THY-7 / Pg3-srfAΔspoIVC, THY-7 / Pg3-srfAΔspoIVF, THY-7 / Pg3-srfAΔspoVA, THY-7 / Pg3-srfAΔspoVB, THY-7 / Pg3-srfAΔspoVD and THY-7 / Pg3-srfAΔspoVE are derived from patent ZL 201811465067.7; Bacillus subtilis THY-7 / Pg3-srfA(leuABCD-ilvK) was obtained from patent ZL 201910549289.5.

[0111] Example 1. Obtaining the lipopeptide composition

[0112] 1. Obtaining compositions containing surfactants

[0113] Select Bacillus subtilis ( B. subtilis A single colony of THY-7 / Pg3-srfA was inoculated into LB liquid medium and cultured at 37°C and 200 rpm for 16 hours to obtain a seed culture. This seed culture was then inoculated into a shake flask of fermentation medium at a ratio of 5% and cultured at 37°C and 200 rpm for 2-6 hours. 1 mM IPTG was then added and the culture was continued for 2 days to obtain a fermentation broth containing surfactant.

[0114] The fermentation medium used consists of: 30-100 g / L sugars, 10-50 g / L inorganic nitrogen source, 0.5-3 g / L organic nitrogen source, 0.1-1 g / L KH2PO4, 0.5-0.3 g / L Na2HPO4·12H2O, 0.002-0.01 g / L CaCl2, 0.002-0.01 g / L MnSO4·H2O, 0.002-0.01 g / L FeSO4·7H2O, pH 6.5-7.5, and fermentation additives (silicone defoamers, polysaccharides, amino acids or their derivatives, oligopeptides, phospholipids, glycolipids, and / or fatty acids or their derivatives) 0.01-20% by weight.

[0115] The surfactant content in the fermentation broth was determined using the method disclosed in CN105400784A. Specifically, 1 mL of fermentation broth was centrifuged at 12000 rpm for 1 minute, and 100 μL of the supernatant was added to 1900 μL of deionized water. After mixing, the mixture was filtered through a 0.22 μm filter and analyzed by HPLC. The mobile phase for HPLC analysis was methanol and water in a volume ratio of 85 / 15, the flow rate was 1 mL / min, the chromatographic column was a C18-ODS reversed-phase column, the column temperature was 40°C, and the detection wavelength was 205 nm using a UV detector.

[0116] The test results showed that the surfactant content in the fermentation broth was approximately 0.001-90 g / L. This fermentation broth is the surfactant-containing composition 1 of the present invention. Surfactants were extracted from the fermentation broth and freeze-dried to obtain a powder product (which can also be directly applied as a composition of the present invention). The powder was then prepared into an aqueous solution of 0.001-100 g / L, which is the composition 2 of the present invention.

[0117] 2. Obtaining a composition containing iturobrine, fenvalerate, and lichenin

[0118] Iridin and fenbufen were purchased from MedChemExpress, and lichenin was purchased from BOC Sciences. They were dissolved in water to prepare aqueous solutions of 0.001-100 g / L, which were used as compositions 3-5 of the present invention.

[0119] In compositions 2-5, 0.01-99.99% by weight of polysaccharides or their derivatives (one or more of peptidoglycan, cellulose, glycogen, starch, chitosan, fructooligosaccharides, polygalactose, and glycosaminoglycans), amino acids (common α-amino acids), oligopeptides (one or more of oligopeptide-3, oligopeptide-4, oligopeptide-5, and oligopeptide-6), lipids (one or more of esters, phospholipids, glycolipids, and cholesterol and their esters), fatty acids or their derivatives (one or more of stearic acid, animal fats, and vegetable oils), and inorganic salts (Na2SO4, K2SO4, (NH4)2SO4, KH2PO4, K2HPO4, Na2HPO4, N One or more of the following: aH2PO4, K3PO4, CO(NH2)2, NH4NO3, NaNO3, CaCl2, FeCl2, NH4Cl, MnSO4, FeSO4, Ca(H2PO4)2; dispersant (one or more of naphthalene sulfonate, lignin sulfonate, polycarboxylate and polycarboxylate ester, starch, bovine serum albumin, cyclodextrin); wetting agent (one or more of dodecyl sulfonate, octyl succinate, alcohol ether sulfate polymer); polymer (one or more of polyethylene glycol, polyacrylic acid emulsion, polyacrylic acid polyvinyl alcohol emulsion, xanthan gum, polyglutamic acid); or warning color reagent as composition 6-8 of the present invention (see Table 1). (Compositions 1-8 can be used directly as agricultural fertilizers or pesticides, or they can be compounded as raw materials for use as agricultural fertilizers, adjuvants or pesticides.) (The above compositions 1-8 can also be applied directly as a powder mixture of the present invention.)

[0120] Table 1.

[0121]

[0122] Example 2. Lipopeptide composition used to improve maize quality and promote maize growth.

[0123] Sweet waxy corn was sown in the field, and after emergence, it was irrigated with a lipopeptide composition. After 31 days of lipopeptide treatment, the control group showed no ear formation, while the lipopeptide-treated group had already formed ears (see [link to relevant documentation]). Figure 7 (See small image 2). After harvesting the corn, measurements showed that the diameter of the corn cob and the number of kernel rows in the lipopeptide-treated group were not significantly different from the control, increasing by only 1.41% and 0.16% respectively. However, the length of the corn cob and the number of kernels per row increased by 11.28%–18.47% compared to the control (see...). Figure 1The study investigated the improvement in corn quality. The soluble sugar content of the treated corn was 1.33%-1.80%, which was 1.99-2.73 times that of the control group (0.66%). The anthocyanin content of the treated corn was 63.1-72.6 mg / 100g, which was 21.3%-39.6% higher than that of the control group (52 mg / 100g). The fatty acid content of the treated corn was 1.05-1.19%, which was 31.2%-48.8% higher than that of the control group (0.8%).

[0124] Example 3. Lipopeptide composition used to improve wheat quality and promote wheat growth.

[0125] The effects of lipopeptides on wheat germination rate and growth vigor were observed using paper culture. The paper bed was thoroughly moistened with water and treatment solution, and seeds were evenly sown in the paper bed. The bed was then placed in a solar incubator, and the germination rate and growth vigor of wheat were observed on days 2, 4, and 7. Table 2 shows that compared with the control group, the treated wheat germinated earlier, had a higher emergence rate, and exhibited stronger growth (see Table 2). Figure 7 (See small image 4) The root system is stronger and the number of fibrous roots has increased (see small image 4) Figure 7 Smaller image 5) Seedlings grow more vigorously (see small image 5) Figure 7 From the small image 6), it can be inferred that it has stronger resistance to adverse conditions during its growth period and higher yield.

[0126] Table 2. Germination rate of wheat in paper culture

[0127]

[0128] Example 4. Lipopeptide composition used to improve the quality of wheatgrass and promote its growth.

[0129] As a superior forage grass in western and plateau regions of my country, crested wheatgrass has an important impact on the development of local animal husbandry. However, the poor climate conditions, low temperature and drought in this area have a significant impact on the early germination rate of crested wheatgrass, and drought during the growth period is also a severe challenge.

[0130] Winged eelgrass seeds were coated with a lipopeptide composition, potted, and watered with 1-200 mL of water. No watering was done during the cultivation process, and the cultivation temperature was maintained at approximately 5 degrees Celsius. The improvement in Winged eelgrass quality was observed. Nine days after sowing, the germination rate in the control group was 10%, while the germination rate in the lipopeptide-treated group was 16.7%-28.0%, indicating that the drought and cold resistance of the lipopeptide-treated group was 6.7%-18.0% higher than that of the control group. Fourteen days after sowing, the germination rate in the treated group increased by 9.3%-12.7% compared to the control group, and the number of tillers increased by 9.33%-37.12%, indicating that lipopeptide treatment further improved the drought and cold resistance of Winged eelgrass compared to the control group. One month after sowing, the chlorophyll content in the leaves of the lipopeptide-treated group increased by 20%-38% compared to the control group.

[0131] In addition, the growth of *Leymus chinensis* was further observed. Fourteen days after sowing, the fresh weight of the aboveground parts in the lipopeptide treatment group increased by 12.5-38.9% compared to the control group (see...). Figure 7 (See small figure 7). One month after sowing, the plant height in the treatment group was 9.8-11.5 cm, an increase of 12.6-32.2% compared to the control group's 8.7 cm. The fresh weight of the above-ground parts in the treatment group was 1.2-1.7 g, an increase of 50-112.5% ​​compared to the control group's 0.8 g. The one-year silage yield in the treatment group was 46.77-80.52% higher than that in the control group.

[0132] Example 5. Lipopeptide composition used to improve oat grass quality and promote oat grass growth.

[0133] Oat grass was sown in pots and then irrigated with lipopeptide composition 1-8. The improvement in oat grass quality was investigated. One month after sowing, the chlorophyll content of leaves in the lipopeptide treatment group increased by 13-22.4% compared with the control group.

[0134] In addition, the growth of oat grass was further observed. Nine days after sowing, the emergence rate of the control group was 44%, while the emergence rate of the lipopeptide treatment group was 58%-81.3%, which was 14-37.3% higher than that of the control group. At 14 days after sowing, the emergence rate of the treatment group was 9.31-20.12% higher than that of the control group, and the root system of the treatment group increased by 24.0%-49% compared with that of the control group (see...). Figure 7 (See small image 8 above). The fresh weight of the aboveground parts increased by 13.52-56.73% compared to the control group. One month after sowing, the plant height in the treatment group was 10.1-11 cm, an increase of 6.3-15.8% compared to the control group's 9.5 cm. The fresh weight of the aboveground parts in the treatment group was 3.2-4.1 g, an increase of 10.3-41.4% compared to the control group (2.9 g) (see...). Figure 7 (See the smaller figure below). The annual silage yield of the treatment group was 11.89-51.25% higher than that of the control group.

[0135] Example 6. Lipopeptide composition for improving sugarcane quality and promoting sugarcane growth.

[0136] Sugarcane was sown in fields with uniform soil fertility, ranging from sandy loam to light clay, and the sugarcane grew evenly. Cultivation and management conditions were consistent throughout the experimental area. Lipopeptide compositions 1-8 were applied to the plants during the tillering, jointing, and 10 days before maturity stages. The improvement in sugarcane quality was observed at harvest. The sugar content of the lipopeptide-treated sugarcane was 7.19%–15.21% higher than that of the control group, and the vitamin content was 5.2%–10.9% higher.

[0137] Example 7. Lipopeptide composition used to improve the quality of crape myrtle and promote its growth.

[0138] Seeds of *Lysimachia christinae* were coated with lipopeptide compositions 1-8, potted and watered with 1-200 mL of water. The improvement in quality was investigated. Ten days after sowing, the chlorophyll content in the leaves of the lipopeptide-treated group increased by 10.3-19.6% compared to the control group. One month after sowing, the protein content in the *Lysimachia christinae* of the lipopeptide-treated group was 11.6-15.1%, an increase of 5.3-8.8% compared to the control group, and the fat content in the *Lysimachia christinae* of the lipopeptide-treated group increased by 2.3-4.5% compared to the control group.

[0139] Example 8. Lipopeptide composition used to improve the quality and promote the growth of *Duckbilleria*.

[0140] After sowing *Chaenomeles speciosa* seeds in the field, the plants were irrigated with lipopeptide compositions 1-8, and the improvement in *Chaenomeles speciosa* quality was investigated. Two months after sowing, the protein content of *Chaenomeles speciosa* in the lipopeptide-treated groups increased by 1.0-4.4% compared to the control group.

[0141] In addition, the growth of *Duckbilleria* was further observed. One month after sowing, the average number of tillers in the treatment group was 13-17, which was 30%-70% higher than the 10 tillers in the control group. The average height of *Duckbilleria* seedlings in the treatment group was 75-93 cm, while the seedling height in the control group was 63 cm, representing an increase of 19.1%-50%. Two months after sowing, the fresh weight of the above-ground parts in the treatment group increased by 31-68% compared to the control group. The one-year silage yield of the treatment group was 50-82.5% higher than that of the control group.

[0142] Example 9. Lipopeptide composition used to improve the quality and promote the growth of *Verbena officinalis*.

[0143] After sowing *Verbena officinalis* seeds in the field, the plants were irrigated with lipopeptide composition 1-8, and the improvement in *Verbena officinalis* quality was investigated. One month after sowing, the protein and fat content of *Verbena officinalis* in the lipopeptide treatment group increased by 3.0-6.9% and 1.3-5.8%, respectively, compared with the control group.

[0144] In addition, the growth of *Verbena officinalis* was further observed. Five days after sowing, the germination rate in the water control group was 63%, while the germination rate in the lipopeptide treatment group was 81.3%-92.7%, an increase of 18.3%-29.7% compared to the control group. One month after sowing, the *Verbena officinalis* in the water group only tillered once, while the average number of tillers in the treatment group was 1.8. The average height of the *Verbena officinalis* in the treatment group was 97-121 cm, while the height in the control group was 83 cm, an increase of 16.9%-45.8% compared to the control group. The fresh weight of the above-ground parts in the treatment group increased by 22.5%-50.8% compared to the control group. The control group was harvested 5 times a year, while the treatment group was harvested an average of 7 times a year. The annual silage yield of the treatment group was 72-110% higher than that of the control group.

[0145] Example 10. Lipopeptide composition used to improve spinach quality and promote spinach growth.

[0146] Spinach was sown in the field, with a planting area of ​​20 m. 2 In each plot, a 50 cm protective row was set between each plot. After emergence, at the 3-4 leaf stage, a lipopeptide composition solution (1-8) was sprayed on the leaves. After approximately 7 days, the solution was sprayed again, for a total of two applications. Spinach was harvested 30 days later, and the improvement in quality was assessed. The average chlorophyll content of the spinach in the lipopeptide treatment group was 37.4-44.5 SPAD, an increase of 16.1%-38.2% compared to the control group; the total nitrogen content was 14.9-18.7 mg / g, an increase of 34.7%-68.5% compared to the control group; and the vitamin content was 1.54-1.89 mg / 100g, an increase of 4.8%-28.6% compared to the control group (see [reference]). Figure 3 The average yield of the treatment groups was 7337.6-7968 g, an increase of 1.7%-10.5% compared to the control group (see [reference]). Figure 7 Small image 9).

[0147] Example 11. Lipopeptide composition used to improve the quality and promote the growth of Chinese cabbage.

[0148] Chinese cabbage is planted in the field, with a planting area of ​​20 m. 2 / plot. Foliar spraying of lipopeptide composition 1-8 was applied twice, during the seedling and vigorous growth stages. The pakchoi was harvested, and the improvement in quality was assessed. As shown in Table 3, the average soluble sugar content of the pakchoi in the lipopeptide treatment group was 3.8 mg / g, which was 119.8% of the control group; the average vitamin content of the pakchoi in the lipopeptide treatment group was 0.94 mg / g, which was 148.5% of the control group.

[0149] In addition, the growth of the bok choy was further observed. The average plant height of the lipopeptide treatment group was 29.50 cm, which was 103.8% of that of the control group. The yield of the lipopeptide treatment group increased by an average of 13.77% compared with the control group (see Table 3 and...). Figure 7 (Small image 10).

[0150] Table 3. Soluble sugar content, plant height, and yield increase rate of Chinese cabbage

[0151]

[0152] Example 12. Lipopeptide composition used to improve tomato quality and promote tomato growth.

[0153] Tomatoes are grown in greenhouses, with a planting area of ​​10 m. 2 / plot. Tomato plants were treated with a foliar spray of lipopeptide composition 1-8. The pesticide was applied twice, once during the seedling stage and once during the flowering stage, for a total of two applications. The entire tomato plant was sprayed evenly using a conventional method. The improvement in tomato quality was then assessed. The average lycopene content in the lipopeptide-treated group was 12.8-14.1 mg / 100g, which was 7.6%-18.5% higher than that in the control group.

[0154] In addition, the growth of tomatoes was further observed. The average plant height in the lipopeptide treatment group was 42.3-46.8 cm, which was 5.8%-17% higher than that in the control group. The average number of flowers in the lipopeptide treatment group was 14.6-16.9, which was 12.3%-30% higher than that in the control group. The average number of fruits in the lipopeptide treatment group was 12.5-15.6, which was 8.7%-35.7% higher than that in the control group. The average weight of a single fruit in the lipopeptide treatment group was 234.1-256.6 g, which was 6.4%-16.6% higher than that in the control group.

[0155] Example 13. Lipopeptide composition used to improve chili pepper quality and promote chili pepper growth.

[0156] Chili peppers are grown in a greenhouse, with a planting area of ​​10 m. 2 / Local area. Foliar spraying of lipopeptide composition 1-8 onto pepper plants. Apply the pesticide once during the seedling stage and once during the flowering stage, for a total of two applications. The entire pepper plant should be sprayed evenly using conventional methods. The improvement in pepper quality was then assessed. The vitamin content, fatty acid content, and carotenoid content of peppers in the lipopeptide treatment group increased by 15.6-27.1%, 3.4-7.9%, and 11.8-14.6%, respectively, compared to the control group.

[0157] In addition, the growth of the chili peppers was further observed. The average plant height in the lipopeptide treatment group was 45.8-47.2 cm, which was 6.5%-9.8% higher than that in the control group. The average number of flowers in the lipopeptide treatment group was 26.3-30.6, which was 9.6%-27.5% higher than that in the control group. The average number of fruits in the lipopeptide treatment group was 24.8-28.3, which was 12.7%-28.6% higher than that in the control group.

[0158] Example 14. Lipopeptide composition used to improve cucumber quality and promote cucumber growth.

[0159] Cucumbers are grown in a greenhouse, with a planting area of ​​10 m. 2 / In a small-scale planting area, cucumber plants were irrigated with 1-200 mL of a lipopeptide composition (1-8). The irrigation was performed once after the cucumber seedlings had established themselves, and then again after a 10-day interval. The improvement in cucumber quality was then assessed. The soluble sugar content of cucumbers in the lipopeptide-treated group was 2.7-3.5%, which was 0.69-1.49% higher than the control group. The protein content of the lipopeptide-treated group was 1.56-1.83%, an increase of 0.52-0.69% compared to the control group. The vitamin C content of the lipopeptide-treated group was 173-196 mg / kg, compared to 127 mg / kg in the control group, an increase of 36.2-54.3% (see [reference needed]). Figure 4 ).

[0160] In addition, the growth of cucumbers was further observed. The average plant height in the lipopeptide treatment group was 160.8-171.5 cm, which was 3.7%-10.6% higher than that in the control group. The average number of flowers in the lipopeptide treatment group was 8-10, which was 14.3%-42.9% higher than that in the control group. The average number of fruits in the lipopeptide treatment group was 6.5-7.6, which was 22.6%-43.4% higher than that in the control group. The average fruit weight in the lipopeptide treatment group was 312.1-350.6 g, which was 4%-16.9% higher than that in the control group (see...). Figure 7 Small image 11).

[0161] Example 15. Lipopeptide composition used to improve bitter melon quality and promote bitter melon growth.

[0162] Transplanting bitter gourd in open fields, planting area 10 m 2 / In a residential area, bitter gourd seedlings were irrigated with 1-200 mL of a lipopeptide composition (1-8) after transplanting and allowing them to establish themselves. The improvement in bitter gourd quality was assessed. In the lipopeptide-treated group, the first female flower bloomed and the first fruit set occurred 6 days earlier than the control. Two months after treatment, the average number of flower buds per plant in the treated group was 61, an increase of 56.4% compared to the control, and each plant yielded 3 bitter gourds, twice the number of the control.

[0163] Example 16. Lipopeptide composition used to improve the quality and promote the growth of winter melon.

[0164] Winter melon seedlings were transplanted into the field and irrigated with 1-200 mL of a lipopeptide composition (1-8) one week after transplanting. The effects of the lipopeptides on flowering, fruiting, and quality of the winter melon were investigated. The first female flower appeared on February 7th in the treatment group, 6 days earlier than the control group, and the first fruit appeared on February 10th, 14 days earlier than the control group (see [link to treatment]). Figure 5 The average amino acid content of the winter melons harvested in the treatment group was 0.61-0.68 g / 100g, which was 35.6-51.1% higher than that in the control group. The average vitamin C content of the winter melons harvested in the treatment group was 14.5-15.1 mg / 100g, which was 9.0%-13.5% higher than that in the control group (13.3 mg / 100g).

[0165] Example 17. Lipopeptide composition used to improve strawberry quality and promote strawberry growth.

[0166] Strawberries are grown in greenhouses, with a planting area of ​​10 m. 2 / Community. Foliar spraying of lipopeptide composition 1-8 onto strawberry plants, applying the solution evenly to the entire plant during the early and peak flowering stages. After spraying the lipopeptide solution, the improvement in strawberry quality was measured (see [reference]). Figure 6 The number of deformed strawberries in the lipopeptide-treated group was 0.33-0.45, while the number of normal-shaped strawberries was 10%-34% higher than that in the control group. The average soluble sugar content in the lipopeptide-treated group was 13.5-14.9 g / 100g, 7%-18.3% higher than that in the control group. The average protein content in the lipopeptide-treated group was 1.63-1.84 g / 100g, 19.8%-35.3% higher than that in the control group. The average pectin content in the lipopeptide-treated group was 0.73-0.92 g / 100g, 25.8%-58.6% higher than that in the control group.

[0167] In addition, the growth of the strawberries was further observed (see...). Figure 6 The lipopeptide treatment group exhibited earlier initial flowering and fruiting times than the control group by one week; the number of fruits in the treatment group was significantly higher than that in the control group; and the fruit maturity was uniform across the treatment groups. The average number of flowers in the lipopeptide treatment group was 12.9-15.35, 2.5%-22% higher than the control group. The average number of fruits in the lipopeptide treatment group was 5.5-5.9, 4.5%-12.4% higher than the control group. The total fruit weight in the lipopeptide treatment group was 150.1-202.6 g, 11.3%-50.2% higher than the control group.

[0168] Example 18. Lipopeptide composition used to improve apple quality and promote apple growth.

[0169] Apple seedlings were soaked in water and a lipopeptide treatment solution for 24 hours before being planted in planting pits. After one month of acclimatization, lipopeptide composition 1-8 was sprayed on the leaves, and sprayed again after two weeks. In the second year, the lipopeptide treatment solution was sprayed at the early leaf emergence stage, rapid growth stage, early flowering stage, and full bloom stage, and the improvement in apple quality was measured. The results showed that the soluble sugar content of apples in the control group was 12 g / 100g, while the average soluble sugar content of apples in the lipopeptide treatment group was 14-16.1 g / 100g, which was 116.7-134.2% of that in the control group. The average protein content of the lipopeptide treatment group was 157%-178% of that in the control group (0.2 g / 100g), and the number of normal-shaped fruits in the lipopeptide treatment group was 9%-20% higher than that in the control group.

[0170] Example 19. Lipopeptide composition used to improve the quality and promote the growth of money trees.

[0171] Select money trees of similar growth and transplant them into self-watering pots. Fill the pots with a lipopeptide treatment solution (1-8). Replace the solution with fresh lipopeptide every seven days. After 21 days of treatment, observe the improvement in the quality of the money trees. The chlorophyll content of the money trees in the lipopeptide treatment group was 68.9-74 mg / g, an increase of 6%-15% compared to the control group. The total nitrogen content of the treatment group was 13.3-16.3 mg / g, an increase of 10.8%-35.8% compared to the control group.

[0172] In addition, the growth of the money tree was further observed. The lipopeptide treatment group had 20-28 leaves, with an average of 5-10 new leaves, representing a 10%-50% increase in the number of new leaves compared to the control group (see [reference]). Figure 7 Small image 13).

[0173] Example 20. Lipopeptide composition used to improve the quality and promote the growth of pothos.

[0174] Select pothos plants of similar growth and insert them into flowerpots. Fill the pots with a lipopeptide mixture (1-8). Water with fresh lipopeptide treatment solution every fifteen days. After 30 days of treatment, assess the improvement in pothos quality. The chlorophyll content of the lipopeptide-treated group was 35.5-42.1 mg / g, which was 111%-132% of the control group; the total nitrogen content of the treated group was 24.2%-39.6% higher than the control group.

[0175] In addition, the growth of the pothos was further observed. The lipopeptide-treated group had 15-20 leaves, with an average of 3-5 new leaves, representing a 50%-150% increase in new leaves compared to the control group (see [reference needed]). Figure 7 Small image 14).

[0176] Example 21. Lipopeptide composition used to improve dahlia quality and promote dahlia growth.

[0177] Trim the dried roots of the dahlia tubers, soak the tubers in a 0.1% carbendazim solution for 10 minutes, rinse once with deionized water, and drain. Sterilize the culture soil, place it in a culture tray, plant the bulbs, and cover the tubers with soil. Irrigate with 1-150 ml of lipopeptide composition 1-8. After 45 days of lipopeptide treatment, observe the improvement in dahlia quality. The chlorophyll and total nitrogen content of the dahlias in the lipopeptide treatment group increased by 7.5-12.3% and 12.1-17.4%, respectively, compared to the control group.

[0178] In addition, the growth of dahlias was further observed. The germination rate of dahlias in the lipopeptide treatment group was on average 50%-100%, which was 40%-90% higher than that in the control group. The average plant height in the lipopeptide treatment group was 5.5-18.9 cm, which was 10%-278% higher than that in the control group (see...). Figure 7(Small image 15).

[0179] Example 22. Lipopeptide composition used to improve daffodil quality and promote daffodil growth.

[0180] Select narcissus bulbs of uniform size, remove the protective soil, old roots, and dried stem segments from the base of the bulbs, soak the bulbs in clean water for 3 hours, and rinse them thoroughly. Place the bulbs in a culture dish, add lipopeptide composition 1-8, and incubate in the lipopeptide treatment solution for 30 days. Observe the improvement in narcissus quality. The total nitrogen content of the narcissus leaves in the lipopeptide treatment group increased by 5.1-9.0% compared with the control group.

[0181] In addition, the growth of daffodils was further observed. The average plant height of daffodils in the lipopeptide treatment group was 9.9-24.7 cm, an increase of 12.5%-180.7% compared to the control group. The average leaf width of daffodils in the lipopeptide treatment group was 1.1-1.3 cm, an increase of 22%-44% compared to the control group (see...). Figure 7 (smaller images 16a and 16b).

[0182] Example 23. Lipopeptide composition used to improve the quality of freesia and promote its growth.

[0183] Select freesia bulbs of uniform size, remove the outer layer of old stem segments, and rinse the bulbs thoroughly with clean water. Place the bulbs in a culture bottle, add lipopeptide composition 1-8, and incubate in the lipopeptide treatment solution for 10 days. Observe the improvement in freesia quality. The flowering period of freesias in the lipopeptide treatment group was on average 2-4 days earlier and 3-5 days longer than that in the control group.

[0184] In addition, the growth of freesias was further observed. The average number of shoots in the lipopeptide-treated group was 15-18, an increase of 7%-28.6% compared to the control group. The average plant height in the lipopeptide-treated group was 6.7-14.2 cm, an increase of 5%-121.9% compared to the control group (see...). Figure 7 (smaller figures 17a and 17b).

[0185] Example 24. Lipopeptide composition used to improve rose quality and promote rose growth.

[0186] In a field rose plantation, after the first harvest, a lipopeptide composition (1-8) was sprayed on the leaves. Seven days later, a second application of the treatment solution was performed, and the improvement in rose quality was observed. The flowering period of the roses in the lipopeptide treatment group was on average 3-5 days earlier than that in the control group.

[0187] In addition, the growth of the roses was further observed. The average height of the flower branches in the lipopeptide treatment group increased by 15%-31% compared with the control group, and the average stem thickness increased by 2 mm compared with the control group.

[0188] Example 25. Lipopeptide composition for improving carnation quality and promoting carnation growth.

[0189] In a carnation plantation, a lipopeptide composition 1-8 was sprayed on the leaves, with a one-week interval between applications, for a total of four applications. The improvement in carnation quality was observed. The flowering period of the control group was four months, while the flowering period of the carnations in the lipopeptide treatment group was extended by an average of one month.

[0190] In addition, the growth of carnations was further observed. The control group had 4 flower branches, while the treatment group had an average of 5-7 flower branches, which was 25-75% more than the control group. The height of the flower branches in the treatment group was 14.2-21.6% higher than that in the control group.

[0191] Example 26. Lipopeptide composition used to improve the quality and promote the growth of Cymbidium faberi.

[0192] Cymbidium faberi was planted in tall pots. After the seedlings had recovered from transplant shock, they were sprayed with a mixture of water and lipopeptide solution at concentrations 1-8. Seven days later, a second foliar spray was applied, for a total of four sprays. The improvement in the quality of the Cymbidium faberi was observed. The flowering period of the control group was 3.5 months, while the flowering period of the treatment group was 4.5-5 months on average, an extension of 1-1.5 months on average.

[0193] In addition, the growth of Cymbidium faberi was further observed. The average number of leaves in the control group was 13, while the average number of leaves in the treatment group was 16-22, an increase of 23.1-69.2% compared to the control group; the number of flowering branches in the control group was 1 per plant, while the average number of flowering branches in the treatment group was 1.5-2.1 per plant, an increase of 50%-110% compared to the control group.

[0194] Example 27. Lipopeptide composition used to improve the quality of perilla and promote its growth.

[0195] After treating perilla seeds with lipopeptide composition 1-8, they were planted in pots and cultivated in soil, watered with 1-200 mL of water, and perilla leaves were harvested 28 days later to investigate quality. The soluble reducing sugar content in the perilla leaves of the lipopeptide-treated group was 1242-1279 mg / 100g, an increase of 8-11.2% compared to the control group; the vitamin C content in the leaves of the lipopeptide-treated group was 5.1-5.6g / 100g, an increase of 10.9-21.7% compared to the control group; and the carotenoid content in the leaves of the lipopeptide-treated group was 10.4-12.0 mg / 100g, an increase of 15.6-33.3% compared to the control group.

[0196] In addition, the growth of perilla was further measured. The average number and weight of leaves in the lipopeptide treatment group increased by 2.2-10.1% and 11.6-20.5% respectively compared with the control group.

[0197] Example 28. Lipopeptide composition used to improve the quality of catnip and promote its growth.

[0198] After mixing Nepeta cataria seeds with lipopeptide composition 1-8, the seeds were planted in pots and cultured in soil, watered with 1-200 mL of water. The Nepeta cataria was harvested after 4 months, and its quality was investigated. The vitamin C content in the leaves of the lipopeptide-treated group was 27.9-32.2 mg / 100g, an increase of 11.6-28.8% compared to the control group; the protein and carotene content in the leaves of the lipopeptide-treated group increased by 4.3-10.5% and 11.0-26.9%, respectively, compared to the control group.

[0199] In addition, the growth of *Nepeta cataria* was further measured. The average fresh weight of plants in the lipopeptide treatment group increased by 7.2-30.2% compared with the control group.

[0200] Example 29. Lipopeptide composition used to improve sweet potato quality and promote sweet potato growth.

[0201] Transplanting sweet potato seedlings in the field, planting area 20 m 2 After the seedlings have established themselves, the plants are irrigated with 1-200 mL of a lipopeptide composition (1-8). Foliar spraying is applied every two weeks, followed by regular, even spraying of the entire plant, for a total of two applications. Sweet potatoes are harvested four months after planting, and the improvement in quality is assessed. The protein content in the lipopeptide-treated group was 1.3-1.6 g / 100g, an increase of 11.1-36.8% compared to the control group; the vitamin content in the lipopeptide-treated group was 25.1-27.8 g, an increase of 21.8-34.9% compared to the control group.

[0202] In addition, the harvest of sweet potatoes was further measured. The yield and weight of sweet potatoes per acre increased by 3.3-21.3% and 2.0-9.8% respectively compared with the control group.

[0203] Example 30. Lipopeptide composition for improving barley quality and promoting barley growth.

[0204] The barley seeds were treated with lipopeptide compositions 1-8, fertilizer, and other ingredients before being sown in the field. The soil fertility was uniform, and cultivation management conditions were consistent throughout the experimental area. 136 days after sowing, the barley matured, was harvested, and the improvement in barley quality was assessed. The results showed that the total dietary fiber content of the barley in the lipopeptide-treated group was 19.9-22.7 g / 100g, an increase of 20.6-37.6% compared to the control group; the vitamin content was 301-343 mg / 100g, an increase of 3.4-18.3% compared to the control group; and the anthocyanin content was 5.5-9.1% higher than the control group.

[0205] In addition, the production of highland barley was further measured, and the average yield per mu of highland barley increased by 9.1-21.5% compared with the control group.

[0206] Example 31. Lipopeptide composition used to improve buckwheat quality and promote buckwheat growth.

[0207] Buckwheat seeds were treated with a mixture of lipopeptide composition 1-8, an insecticide, a fungicide, and then sown in the field. The planting field had uniform fertility, and cultivation management conditions were consistent throughout the experimental area. The buckwheat matured 95 days after sowing, was harvested, and its quality was measured. The results showed that the protein, vitamin, carotene, and dietary fiber contents of the buckwheat in the lipopeptide treatment group were increased by 19.4-23.6%, 10.0-13.7%, 10.4-13.1%, and 8.5-9.7%, respectively, compared to the control group.

[0208] In addition, buckwheat production was further measured, and the average yield per mu increased by 6.9-22.3% compared with the control group.

[0209] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention in any way. Although specific embodiments of the present invention have been described above, those skilled in the art should understand that these are merely illustrative examples, and the scope of protection of the present invention is defined by the appended claims. Those skilled in the art can make various changes or modifications to the above-disclosed technical content without departing from the scope of the present invention, and all such changes and modifications fall within the scope of protection of the present invention.

Claims

1. Use of a composition for improving plant quality, wherein the composition comprises: (i) lipopeptides in the range of 0.001–100 g / L; and (ii) Additives, The plants mentioned are non-leguminous plants and are selected from the following group: grain plants, livestock plants, vegetable plants, fruit plants, horticultural plants, and flowering plants. The improvement in plant quality is characterized by the following: (a) Improve the content of soluble sugars, anthocyanins, fatty acids, proteins, dietary fiber, carotene and / or vitamins in grain plants; (b) Improve cold resistance, fat content and / or protein content in livestock plants; (c) Improve total nitrogen content, vitamin content, soluble sugar content, lycopene content, fatty acid content, carotenoid content, protein content, growth cycle and / or amino acid content in vegetable plants; (d) Improve soluble sugar, protein, and / or pectin content in fruit plants; or (e) Improve total nitrogen levels and / or growth cycles in horticultural and flowering plants.

2. Use of a composition for improving plant quality and promoting plant growth, wherein the composition comprises: (i) lipopeptides in the range of 0.001–100 g / L; and (ii) Additives, The plants mentioned are non-leguminous plants and are selected from the following group: grain plants, livestock plants, vegetable plants, fruit plants, horticultural plants, and flowering plants. The plant quality is characterized by the following: (a) Improve the content of soluble sugars, anthocyanins, fatty acids, proteins, dietary fiber, carotene and / or vitamins in grain plants; (b) Improve cold resistance, fat content and / or protein content in livestock plants; (c) Improve total nitrogen content, vitamin content, soluble sugar content, lycopene content, fatty acid content, carotenoid content, protein content, growth cycle and / or amino acid content in vegetable plants; (d) Improve soluble sugar, protein, and / or pectin content in fruit plants; or (e) Improve total nitrogen levels and / or growth cycles in horticultural and floricultural plants. Furthermore, the promotion of plant growth described herein is characterized by the following: (a) Improve germination rate, aboveground fresh weight, plant height and / or plant yield in grain plants; (b) Improve seedling emergence rate, tiller number, aboveground fresh weight, plant height and / or plant yield in livestock plants; (c) Improve plant height, number of flowers (branches), single fruit weight, number of fruits, fruit weight and / or plant yield in vegetable plants; (d) In fruit plants, improve the number of flowers (branches), the number of fruits, and / or the weight of fruits; or (e) Improve leaf number, emergence rate, plant height, leaf width, number of buds, flower branch height, flower stem thickness, number of flowers (branches) and / or leaf weight in horticultural and flowering plants.

3. The use according to claim 1 or 2, wherein the composition is further used as an agricultural fertilizer, agricultural adjuvant, or pesticide.

4. The use according to claim 1 or 2, wherein the lipopeptide is a cyclic lipopeptide or a linear lipopeptide, wherein the cyclic lipopeptide has one of the following formulas (I)-(III): (I) (II) (III) Where R represents the carbon chain of the fatty acid chain, A1, A2, A3…A m These represent the 1st, 2nd, 3rd...mth amino acids on the peptide chain, respectively. The carboxyl group of the fatty acid is attached to the N-terminus of A1. m The C-terminal carboxyl group is linked to the hydroxyl or amino groups of other amino acids in the fatty acid or peptide chain to form a cyclic structure; The linear lipopeptide is the linear product obtained by opening the cyclic lipopeptide.

5. The use according to claim 1 or 2, wherein the lipopeptide is one or more selected from the group consisting of surfactant families, iturobrine families, and fenestrated mustard families.

6. The use according to claim 1 or 2, wherein the lipopeptide is one or more selected from the group consisting of: surfactants, irisin, fenbufen, lichenin, babylin, permiracin, spore-forming fungicides, antifungal fungicides, phosphatidylin, fusarium fungicides, kurstakin, spore-forming fungicides, polymyxins, octapeptide, ganzoin, and polypeptide fungicides.

7. The use according to claim 1 or 2, wherein the lipopeptide is produced by bacteria, actinomycetes, fungi or cyanobacteria.

8. The use according to claim 7, wherein the bacteria are selected from the group consisting of Bacillus, Pseudomonas, Streptomyces, Arthrobacter, and Burkholderia; the actinomycetes are selected from the group consisting of Streptomyces, Acidobacterium, and Microbacterium; the fungi are selected from the group consisting of Aspergillus, Microcystis, Erythromyces, Erythromyces, Micrococcus, and Mimosa; and the cyanobacteria are selected from the group consisting of Marine Cyanobacteria, Litmus, and Penicillium.

9. The use according to claim 7, wherein the lipopeptide is obtained by culturing Bacillus or Pseudomonas.

10. The use according to claim 7, wherein the lipopeptide is obtained by culturing Bacillus and regulating the expression of lipopeptide synthesis-related genes, wherein the lipopeptide synthesis-related genes are selected from the group consisting of: lipopeptide synthesis genes, transmembrane transport protein gene ycxA, biotin carboxylase gene yngH, spore synthesis genes spoIVA / B / C / F, spoVA / B / D / E, and leucine synthesis pathway genes leuABCD / ilvK.

11. The use according to claim 10, wherein the lipopeptide synthesis gene is selected from the group consisting of: surfactant synthesis gene srfA, fen mustard synthesis gene fen, lichenin synthesis gene lic, bam mycotoxin synthesis gene bam, antifungal subtilisin myc, iturin synthesis gene itu, phosphatidylin synthesis gene pps, fusarium oxysporin fuscule ...

12. The use according to claim 1 or 2, wherein the composition further comprises 0.01-99.99% by weight of one or more components selected from the group consisting of: polysaccharides, oligosaccharides, dextrins, amino acids, oligopeptides, lipids, proteins, and fatty acids or derivatives thereof, inorganic salts, dispersants, wetting agents, and polymers.

13. The use according to claim 1 or 2, wherein the composition is a fermentation broth containing lipopeptides, an aqueous solution, a concentrate, or a partially purified and dried powder.

14. The use according to claim 1 or 2, wherein the composition is a culture medium obtained by culturing bacteria, actinomycetes, fungi and cyanobacteria capable of producing lipopeptides.

15. The use according to claim 1 or 2, wherein the grain or livestock plant is a grass plant; the vegetable plant is selected from the group consisting of: Solanaceae, Brassicaceae, Chenopodiaceae, Asteraceae, Apiaceae, Cucurbitaceae, Lamiaceae, Malvaceae, and Convolvulaceae; the fruit plant is selected from the group consisting of: strawberry, blueberry, raspberry, cranberry, cherry, peach, plum, jujube, apple, pear, citrus, orange, grapefruit, and banana; the horticultural plant is selected from the group consisting of: money tree, spider plant, pothos, wandering jenny, money tree, lucky bamboo, money tree, banyan tree, evergreen, and dwarf coconut palm; and the flowering plant is selected from the group consisting of: daffodil, dahlia, freesia, iris, peony, peony, tulip, hyacinth, lily of the valley, hosta, bellflower, lily of the valley, asters, daylily, orchid, rose, rose, carnation, chrysanthemum, wild rose, and bellflower.

16. The use according to claim 15, wherein the vegetable plant is selected from the group consisting of: eggplant, potato, tomato, pepper, lettuce, bok choy, Chinese cabbage, radish, rapeseed, spinach, celery, cucumber, loofah, bitter melon, pumpkin, winter melon, watermelon, cantaloupe, honeydew melon, perilla, catnip, mint, and okra.

17. The use according to claim 15, wherein the grain plant or livestock plant is selected from the group consisting of: wheat, rice, upland rice, corn, oats, rye, barley, buckwheat, millet, sorghum, highland barley, wild rice, taro, Job's tears, sugarcane, sugar beet, sweet potato, oat grass, *Gnaphalium affine*, *Isodon japonicus*, *Phyllostachys edulis*, *Leymus chinensis*, *Leymus chinensis*, barley grass, new barley grass, *Rhizophora stylosa*, *Cynodon dactylon*, *Tripterygium wilfordii*, *Miscanthus sinensis*, *Duckbillella asiatica*, *Eriocaulon buergerianum*, *Lysimachia nummularia*, *Indigofera tinctoria*, *Isodon sibirica*, *Sorghum bicolor*, *Eriocaulon buergerianum*, alfalfa, and *Imperata cylindrica*.

18. A method for improving plant quality, comprising: (1) Adding the composition defined in any one of claims 1-14 to the culture of the plant; (2) Cultivate and harvest the plant in the culture of (1); (3) Optionally, measure the quality parameters of the cultured plants and / or their fruits and compare them with the quality parameters of plants and / or their fruits cultured in a culture that does not contain the said lipopeptides. The plants mentioned are non-leguminous plants and are selected from the following group: grain plants, livestock plants, vegetable plants, fruit plants, horticultural plants, and flowering plants. The plant quality is characterized by the following: (a) Improve the content of soluble sugars, anthocyanins, fatty acids, proteins, dietary fiber, carotene and / or vitamins in grain plants; (b) Improve cold resistance, fat content and / or protein content in livestock plants; (c) Improve total nitrogen content, vitamin content, soluble sugar content, lycopene content, fatty acid content, carotenoid content, protein content, growth cycle and / or amino acid content in vegetable plants; (d) Improve soluble sugar, protein, and / or pectin content in fruit plants; or (e) Improve total nitrogen levels and / or growth cycles in horticultural and flowering plants.

19. The method of claim 18, wherein the improvement in plant quality is characterized by the following: The quality parameters of plants cultured with the addition of the composition are at least 110%, at least 120%, at least 130%, at least 140%, or at least 150% of those of the control group without the addition of the composition.

20. The method according to claim 18 or 19, wherein the growth of the plant is simultaneously promoted, and the promotion of growth is characterized by the following: (a) Improve germination rate, aboveground fresh weight, plant height and / or plant yield in grain plants; (b) Improve seedling emergence rate, tiller number, aboveground fresh weight, plant height and / or plant yield in livestock plants; (c) Improve plant height, number of flowers (branches), single fruit weight, number of fruits, fruit weight and / or plant yield in vegetable plants; (d) In fruit plants, improve the number of flowers (branches), the number of fruits, and / or the weight of fruits; or (e) Improve leaf number, emergence rate, plant height, leaf width, number of buds, flower branch height, flower stem thickness, number of flowers (branches) and / or leaf weight in horticultural and flowering plants.

21. The method of claim 20, wherein the promotion of plant growth is characterized by the following: The growth parameters of plants cultured with the addition of the composition are at least 110%, at least 120%, at least 130%, at least 140%, or at least 150% of those of the control group without the addition of the composition.

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