Application of a mixed bacterial fermentation broth in decolorization of triphenylmethane dyes and strains

By constructing a mixed bacterial fermentation broth, the problem of difficult treatment of tritylmethane dye sewage is solved, and efficient and broad-spectrum dye decolorization and degradation effects are achieved, with strong adaptability and broad application prospects.

CN116589101BActive Publication Date: 2025-08-01ZHEJIANG UNIV OF TECH
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Patent Information

Application Number
CN202310405119.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-04-17
Publication Date
2025-08-01
Estimated Expiration
2043-04-17

AI Technical Summary

Technical Problem

The prior art is difficult to efficiently remove tritylmethane dye wastewater, especially the decolorization and degradation effect of various tritylmethane dyes, and it is difficult for a single bacteria to adapt to the impact of the natural environment.

Method used

A mixed bacterial fermentation broth was constructed, which was a mixed fermentation broth of Bacillus subtilis, Pseudomonas and Actinomycetes. By optimizing the proportion and culture conditions, the decolorization and degradation efficiency of paratrityl methane dyes was improved.

Benefits of technology

The mixed bacterial fermentation broth can decolorize more than 70% of the water-soluble aniline blue under stand or shake conditions, tolerate pH changes of 4 to 8 and high concentration of water-soluble aniline blue, and the decolorization rate of other tritylmethane dyes such as alkaline magenta and malachite green can reach more than 90%, significantly improving environmental adaptability and degradation range.

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Abstract

The present invention discloses an application of a mixed bacterial fermentation broth in the decolorization of triphenylmethane dyes. The mixed bacterial fermentation broth comprises a mixture of fermentation broths of one or more of Bacillus subtilis, Pseudomonas or Actinomycetes. The mixed bacterial fermentation broth constructed in the present invention for the efficient decolorization of triphenylmethane dyes can decolorize more than 70% of water-soluble aniline blue under both static and shaking conditions, and can tolerate a pH of about 4-8 and a high concentration of water-soluble aniline blue. In addition, the bacterial fermentation broth also has a good decolorization effect on other triphenylmethane dyes. It can decolorize more than 90% of basic fuchsin and malachite green within 48 hours, and also has a certain decolorization effect on three triphenylmethane dyes, namely methyl violet, acid blue 1 and acid red 94. The types of triphenylmethane dyes decolorized are significantly more than those of the reported microbial strains, showing high broad-spectrum property, and having the possibility of being actually applied to solve triphenylmethane dye wastewater.
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