Compositions for enhancing exercise endurance and their applications

Abstract

The present invention belongs to the technical field of medicine and food homologous compositions, and discloses a composition for enhancing exercise endurance and its applications. It is found that the composition has the following effects: (1) After 30 minutes of intervention with a high dose of the composition, exercise endurance can be significantly enhanced, and it can affect exercise endurance by regulating the levels of hormones such as testosterone and cortisol. (2) Intervention with the composition for 14 days can significantly prolong the swimming time to exhaustion under negative gravity in mice, reduce the accumulation of harmful metabolites, thereby increasing the body's energy metabolism and reducing the degree of fatigue of the body. (3) Intervention with a high dose of the composition for 28 days can significantly increase exercise endurance, and by increasing the muscle glycogen reserve and reducing the accumulation of harmful metabolites such as urea nitrogen and lactic acid, the effect of improving exercise endurance is achieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of medicine and food homologous compositions, and more specifically, relates to a composition for enhancing exercise endurance and its applications. Background Art

[0002] Exercise endurance can be defined as the ability to maintain sub-maximal exercise until exhaustion. From the perspective of exercise performance, a decrease in sub-maximal exercise ability means a decrease in endurance. The decrease in exercise endurance is actually a situation where the energy generated by the body during exercise is insufficient to maintain the needs of the muscles, resulting in fatigue and a decrease in exercise performance.

[0003] Improving exercise endurance has important value in real life. Improving exercise endurance can not only meet the needs of athletes to improve their sports performance in competitive competitions, but also meet the needs of ordinary people for improving physical fitness and physical health, as well as the physical needs in special scenarios such as long-term physical labor and battlefields. Past studies have confirmed that improving exercise endurance has the effects of enhancing resistance, strengthening bones and muscles, improving cardiovascular health, and increasing the utilization rate of oxygen by the respiratory system, which is of great benefit to the health status of the body. It is reported that exercise endurance is negatively correlated with the mortality rate of people with cardiovascular diseases, and cardiovascular health is closely related to endurance performance. Cardiovascular diseases are the biggest challenges in global public health today, and cardiovascular diseases cause about one-third of the deaths globally. By improving exercise endurance, cardiovascular and metabolic diseases can be effectively prevented, and improving exercise endurance is of great significance for the prevention and treatment of cardiovascular diseases. As the energy output during exercise increases, the respiratory system must also respond to the increasing oxygen demand of the muscles. Improving exercise endurance can increase the ventilation volume of breathing. Improving exercise endurance can be used as a new strategy to improve the health of the body, and scientific research on methods to improve exercise endurance has important theoretical value and practical significance.

[0004] Currently, the intervention means for enhancing exercise endurance are mainly divided into physical means and chemical means. Among them, physical means include cold exposure, electrical stimulation, massage, laser therapy, etc. Although physical intervention can significantly improve the fatigue state after exercise and reduce the injuries caused by exercise, physical intervention means are limited by equipment, time, and suitable places, and are often used for the exercise repair and endurance enhancement of professional athletes, with a relatively small audience; chemical drugs for enhancing exercise endurance include anabolic steroids, peptide hormones, psychostimulants, β-blockers, diuretics, narcotic analgesics, etc.

[0005] Food source component intervention is more convenient, safe, and has a wider scope of application compared to physical intervention and chemical drug intervention. Currently, there are already literature reports at home and abroad on the research of enhancing exercise endurance through food source components. Food has rich sources and resources, and at the same time, food source components have great development space and application prospects in the field of sports functional foods, which is worthy of in-depth research. And through the research on the mechanism of action of food source components in enhancing exercise endurance, it can provide a scientific basis for the screening of anti-fatigue drugs and sports functional foods, which is conducive to accelerating the development of new functional foods. To sum up, if a new functional food that can safely and effectively enhance exercise endurance can be developed, it will be able to meet people's daily needs for improving sports performance and reduce the safety risks brought by long-term use, providing scientific and effective intervention measures for athletes and fitness enthusiasts. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the problems existing in the prior art and first provide a composition for enhancing exercise endurance.

[0007] The second object of the present invention is the application of the above composition for enhancing exercise endurance.

[0008] The object of the present invention is achieved by the following technical solutions:

[0009] A composition for enhancing exercise endurance, the active ingredients of the composition are composed of the following substances in parts by weight: 25 - 30 parts of allose, 10 - 16 parts of sucrose, 5 - 10 parts of glucose, 12 - 16 parts of 5-hydroxymethylfurfural, 14 - 18 parts of palmitic acid, 14 - 18 parts of linoleic acid, 3 - 5 parts of ginsenoside, and 1 - 3 parts of gingerol.

[0010] The inventive concept of the present invention is as follows: The inventor independently developed a traditional Chinese medicine composition for daily health care and found that it has an obvious effect on improving endurance after exercise, specifically manifested as a significant reduction in fatigue degree after exercise and a significant prolongation of the time to maintain high-intensity exercise. The inventor conducted GC-MS detection and analysis on the traditional Chinese medicine composition and found that the main components of the traditional Chinese medicine composition are: sucrose, allose, palmitic acid, linoleic acid, 5-hydroxymethylfurfural, ginsenoside, and gingerol. Therefore, the inventor combined the main components in the traditional Chinese medicine composition with glucose and compounded them according to the ratio of each component prompted by GC-MS to obtain the composition for enhancing exercise endurance to be protected by the present invention. The obtained composition was used in animal experiments and found to significantly improve exercise endurance.

[0011] Preferably, the composition is composed of the following substances in parts by weight: 28 parts of allose, 12 parts of sucrose, 8 parts of glucose, 12 parts of 5-hydroxymethylfurfural, 18 parts of palmitic acid, 18 parts of linoleic acid, 3 parts of ginsenoside, and 1 part of gingerol.

[0012] The present invention also provides the application of the above composition in the preparation of products for improving exercise endurance.

[0013] Preferably, in the above application, the composition has at least one of the following effects:

[0014] (1) Improve exercise endurance;

[0015] (2) Prolong exercise time;

[0016] (3) Improve the body's energy metabolism;

[0017] (4) Reduce the accumulation of harmful metabolites after exercise;

[0018] (5) Increase the hormone level in serum;

[0019] (6) Reduce muscle damage caused by long-term exercise;

[0020] (7) Increase muscle glycogen reserve.

[0021] More preferably, in the above application, the harmful metabolites are blood urea nitrogen and blood lactic acid.

[0022] More preferably, in the above application, the hormones in serum are testosterone and cortisol.

[0023] More preferably, in the above application, the application concentration of the composition is 36.5 - 146.0 mg / kg·BW.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] In this study, through the intervention of the composition in different time dimensions, the efficacy of the composition in enhancing exercise endurance was systematically evaluated. The negative gravity exhaustion swimming time of mice, the levels of harmful metabolites in serum, the levels of energy substance reserves, the levels of hormones in serum, the level of muscle tissue damage and the overall energy metabolism level were measured to explore the effect of the composition in enhancing exercise endurance and its potential biological mechanism. The specific conclusions are as follows:

[0026] (1) After 30 minutes of intervention with a high dose of the composition, exercise endurance can be significantly enhanced, and it can affect exercise endurance by mobilizing hormone levels such as testosterone and cortisol.

[0027] (2) The 14-day intervention with the composition can significantly prolong the negative gravity exhaustion swimming time of mice and reduce the accumulation of harmful metabolites, thereby increasing the body's energy metabolism and reducing the degree of fatigue.

[0028] (3) The 28-day intervention with a high dose of the composition can significantly increase exercise endurance, and by increasing muscle glycogen reserve and reducing the accumulation of harmful metabolites such as urea nitrogen and lactic acid, the effect of improving exercise endurance is achieved. Description of the Drawings

[0029] Figure 1 Effect of the 30 - minute composition and maca extract on the negative - gravity exhaustion swimming time of mice. Among them, NC represents the blank control group, AC represents the maca positive control group, YDLL represents the low - dose group of the composition, YDLH represents the high - dose group of the composition, and different letters a, b, c represent significant differences between groups (p ≤ 0.05);

[0030] Figure 2 Effect of the 30 - minute composition and maca extract on the levels of energy - reserve substances in mice; the left figure is the effect on liver glycogen, and the right figure is the effect on muscle glycogen. Different letters a, b, c represent significant differences between groups (p ≤ 0.05);

[0031] Figure 3 Effect of the 30 - minute composition and maca extract on the levels of harmful metabolites in mouse serum; the left figure is the effect on blood urea nitrogen, and the right figure is the effect on blood lactic acid. Different letters a, b, c represent significant differences between groups (p ≤ 0.05);

[0032] Figure 4 Effect of the 30 - minute composition and maca extract on the hormone levels in mouse serum. The left figure is the effect on testosterone, and the right figure is the effect on cortisol. * represents significant differences between groups (P < 0.05), and ** represents extremely significant differences between groups (P < 0.01);

[0033] Figure 5 Effect of the 14 - day composition and maca extract on the negative - gravity exhaustion swimming time of mice. Different letters a, b, c represent significant differences between groups (p ≤ 0.05);

[0034] Figure 6 Effect of the 14 - day composition and maca extract on the levels of harmful metabolites in mouse serum. Figure A is for blood urea nitrogen, and Figure B is for blood lactic acid. Different letters a, b, c represent significant differences between groups (p ≤ 0.05);

[0035] Figure 7 Effect of the 28 - day composition and maca extract on the negative - gravity exhaustion swimming time of mice; different letters a, b, c represent significant differences between groups (p ≤ 0.05);

[0036] Figure 8 Muscle tissue sections of mice after endurance exercise for each group. Figure A is the blank control group, Figure B is the maca positive control group, Figure C is the low - dose group of the composition, and Figure D is the high - dose group of the composition;

[0037] Figure 9 Infrared imaging map of the mouse body surface;

[0038] Figure 10 Shows the temperatures of different regions of the mouse body. Figure A is the tail, Figure B is the eye, and Figure C is the back;

[0039] Figure 11 Shows the effects of the composition and maca extract on the levels of energy reserve substances in mice after 28 days. Figure A is liver glycogen, and Figure B is muscle glycogen. Different letters a, b, and c indicate significant differences between groups (p ≤ 0.05);

[0040] Figure 12 Shows the effects of the composition and maca extract on the levels of harmful metabolites in the serum of mice after 28 days. Figure A shows the effect on blood lactic acid, and Figure B shows the effect on blood urea nitrogen. Different letters a, b, and c indicate significant differences between groups (p ≤ 0.05). Detailed implementation mode

[0041] The following will further describe the present invention in detail with reference to the accompanying drawings in the embodiments of the present invention, and the described content will not limit the present invention described in the claims. Obviously, the described embodiments are only key embodiments, not all embodiments, and the content is easy to understand for those skilled in the art.

[0042] Unless otherwise specified, the test methods used in the following experimental examples are all conventional methods; the materials, reagents, etc. used, unless otherwise specified, are reagents and materials that can be obtained from commercial channels.

[0043] Example 1

[0044] A composition for enhancing exercise endurance, comprising the following components in the following parts by weight: 25 parts of allose, 15 parts of sucrose, 10 parts of glucose, 16 parts of 5-hydroxymethylfurfural, 14 parts of palmitic acid, 14 parts of linoleic acid, 4 parts of ginsenoside, and 2 parts of gingerol.

[0045] Example 2

[0046] A composition for enhancing exercise endurance, comprising the following components in the following parts by weight: 30 parts of allose, 12 parts of sucrose, 5 parts of glucose, 15 parts of 5-hydroxymethylfurfural, 15 parts of palmitic acid, 15 parts of linoleic acid, 5 parts of ginsenoside, and 3 parts of gingerol.

[0047] Example 3

[0048] A composition for enhancing exercise endurance, comprising the following components in the following parts by weight: 28 parts of allose, 12 parts of sucrose, 8 parts of glucose, 12 parts of 5-hydroxymethylfurfural, 18 parts of palmitic acid, 18 parts of linoleic acid, 3 parts of ginsenoside, and 1 part of gingerol.

[0049] Example 4

[0050] Composition for enhancing exercise endurance, comprising the following components in parts by weight: allose 28 parts, sucrose 13 parts, glucose 8 parts, 5-hydroxymethylfurfural 13 parts, palmitic acid 16 parts, linoleic acid 16 parts, ginsenoside 4 parts, gingerol 2 parts. I. Effects of 30-minute composition intervention on exercise endurance and hormone levels in mice

[0051] 1. Experimental method

[0052] (1) Treatment of experimental animals

[0053] After 7 days of adaptive feeding of mice, they were randomly divided into 4 groups: blank control group (pure water), maca positive control group (0.45 g / kg·BW), low-dose composition group (0.45 g / kg·BW), high-dose composition group (1.8 g / kg·BW), n = 15, single gavage volume = 0.01 × mouse body weight (g), once only. 30 minutes after gavage, some mice were subjected to a negative gravity exhaustion swimming test (n = 7), with a load of 5% body weight, and the exhaustion swimming time was measured; the remaining mice were subjected to a 90-minute unloaded swimming test (n = 8). After 90 minutes, the mice were fished out, dried, rested for 15 minutes, and then blood was collected from the eye socket. After dissection, muscles and internal organs were collected for subsequent determination of biochemical indexes.

[0054] (2) Detection of liver glycogen and muscle glycogen in mice

[0055] Refer to the prior art.

[0056] (3) Detection of serum urea nitrogen and lactic acid in mice

[0057] Refer to the prior art.

[0058] (4) Detection of serum hormone levels in mice

[0059] Refer to the prior art.

[0060] 2. Experimental results

[0061] (1) Effects on the negative gravity exhaustion swimming time of mice

[0062] In this study, the negative gravity exhaustion swimming time of mice was measured. As Figure 1 shown, compared with the blank control group, the exhaustion swimming time of the high-dose composition group was significantly prolonged by 129.18% (p < 0.05). Compared with the maca positive control, the exhaustion swimming time of the high-dose composition group was significantly prolonged by 73.04%; indicating that composition intervention can immediately and significantly prolong the endurance exercise time.

[0063] (2) Effects on the levels of energy reserve substances in mice

[0064] Compared with the blank control group, there were no significant differences in liver glycogen among the Maca positive control group, the low-dose and high-dose groups of the composition in mice (P>0.05). Compared with the blank control group, the muscle glycogen in the low-dose group of the composition decreased by 2.51%, showing a significant difference (P<0.05). This indicates that the composition can immediately mobilize muscle glycogen reserves to meet immediate energy demands, but it has no significant effect on the reserve and utilization of liver glycogen( Figure 2 ).

[0065] (3) Effects on the accumulation level of harmful metabolites in mice

[0066] Compared with the blank control group, the levels of blood urea nitrogen and blood lactic acid in the high-dose group of the composition in mice decreased by 11.96% and 16.42% respectively, showing statistical significance (P<0.05); compared with the Maca positive control group, the levels of blood urea nitrogen and blood lactic acid in the high-dose group of the composition in mice decreased by 4.28% and 7.83% respectively, indicating that the composition can immediately reduce the accumulation of harmful metabolites during exercise and has a better effect than Maca( Figure 3 )

[0067] (4) Effects on the hormone levels in mouse serum

[0068] Compared with the blank control group, the high-dose group of the composition significantly increased the testosterone level, with an increase of 12.85%, showing a significant difference (P<0.05), and an increase of 5.66% compared with the Maca positive control group. And the high-dose composition extremely significantly increased the cortisol level, with an increase of 20.67% (P<0.01), and an increase of 10.98% compared with the Maca positive control group. This indicates that the high-dose composition can increase the levels of testosterone and cortisol in mouse serum in a short time Figure 4 )

[0069] The research results prove that the composition can improve exercise endurance and extend exercise time 30 minutes after intervention, indicating that the composition has the effect of rapidly improving exercise performance

[0070] II. Effects of 14-day composition intervention on exercise endurance and hormone levels in mice

[0071] 1. Experimental methods

[0072] (1) Treatment of experimental animals

[0073] After 7 days of adaptive feeding, the mice were randomly divided into 4 groups: blank control group (pure water), low-dose composition group (0.45 g / kg·BW), medium-dose composition group (0.9 g / kg·BW), and high-dose composition group (1.8 g / kg·BW), with n = 5. The single gavage volume = 0.01 × mouse body weight (g), once a day for 14 days. After gavage on the last day, the feces of the mice were collected for the negative gravity exhaustion swimming test. The load was 5% of the body weight, and the exhaustion swimming time was measured. The mice were judged to be exhausted when the nose passed under the water surface for 8 s. After the mice were exhausted, they were fished out, dried, and after resting for 15 min, blood was collected from the eyeballs for subsequent biochemical index determination.

[0074] (2) Determination of urea nitrogen and lactic acid levels in mouse serum

[0075] Refer to the prior art.

[0076] 2. Experimental results

[0077] (1) Effect on the negative gravity exhaustion swimming time of mice

[0078] The negative gravity exhaustion swimming time is one of the important indicators for evaluating exercise endurance. As Figure 5 shown, after gavage with the composition for 14 days, compared with the blank control group, the negative gravity swimming times of the low-, medium-, and high-dose groups of mice increased by 61.99%, 22.38%, and 112.95% respectively. There were significant differences between the low- and high-dose groups and the blank control group (P < 0.05). Among them, the negative gravity swimming time of the low-dose group reached an average of 89.00 min, and that of the high-dose group reached an average of 117.00 min.

[0079] (2) Effect on the blood lactic acid and blood urea nitrogen levels of mice

[0080] Both serum urea nitrogen and lactic acid are products of energy metabolism. Excessive accumulation will lead to fatigue and inhibit exercise performance. As Figure 6 shown, compared with the blank control group, the low-, medium-, and high-dose compositions could all significantly reduce blood lactic acid, by 21.46%, 18.07%, and 24.20% respectively. There were significant differences between the low- and high-dose groups and the blank group (P < 0.05). And the low- and high-dose compositions could reduce the blood urea nitrogen level, by 18.14% and 13.89% respectively, with significant differences compared with the blank control group.

[0081] The above research found that the composition intervention for 14 days could significantly prolong the negative gravity exhaustion swimming time of mice, and could reduce the accumulation of harmful metabolites such as blood lactic acid and blood urea nitrogen after endurance exercise in mice, indicating that the composition intervention made the fatigue degree of the mice's body lower than that of the blank group mice after exercise, and the endurance level was higher. This study confirmed that the intervention of low and high doses of the composition for 14 days could effectively increase the exercise endurance of mice and prolong the exercise time.

[0082] III. Effects of 28-day composition intervention on exercise endurance and hormone levels of mice

[0083] 1. Experimental methods

[0084] (1) Treatment of experimental animals

[0085] After 7 days of adaptive feeding of mice, they were randomly divided into 4 groups: blank control group (pure water), Maca positive control group (0.45 g / kg·BW), low-dose composition group (0.45 g / kg·BW), and high-dose composition group (1.8 g / kg·BW), n = 15, single gavage volume = 0.01 × mouse body weight (g), once a day for a total of 28 days. After gavage on the last day, infrared photography was performed, and a thermal imager was used to take pictures 1 m above the mice, with a radiation rate of 0.95. The pictures were processed using FLIR TOOLs software after shooting to obtain data. Then, some mice were subjected to a negative gravity exhaustion swimming test (n = 7), with a load of 5% body weight, and the exhaustion swimming time was measured; the remaining mice were subjected to a 90-minute unloaded swimming test (n = 8). After 90 minutes, the mice were fished out, dried, rested for 15 minutes, and then blood was collected from the eyeballs. The muscles and organs were dissected and collected for subsequent biochemical index determination.

[0086] (2) HE staining of mouse muscle tissue sections

[0087] According to the literature operation, the gastrocnemius muscles of mice were taken out, fixed with 4% formaldehyde tissue fixative for 24 hours, dehydrated step by step with different concentrations of ethanol, and then the tissue was placed in a container filled with paraffin. After the paraffin solidified, sections were obtained using a microtome. The paraffin sections were dewaxed with xylene and then through different concentrations of ethanol until distilled water. Then the sections were soaked in hematoxylin aqueous solution for 5 minutes, washed with water for 1 minute, differentiated with �.5% hydrochloric acid alcohol for 3 - 10 seconds, blued with saturated disodium hydrogen phosphate solution for 10 minutes, dehydrated in 70% and 90% ethanol for 5 minutes each, and finally stained with alcoholic eosin stain for 3 - 30 seconds.

[0088] (3) Determination of urea nitrogen and lactic acid levels in mouse serum

[0089] Refer to the existing technology.

[0090] (4) Detection of liver glycogen and muscle glycogen in mice

[0091] Referring to the prior art.

[0092] 2. Experimental results

[0093] (1) Effect of the composition intervention for 28 days on the endurance exercise time of mice

[0094] To determine whether the composition can effectively extend the endurance exercise time of mice, the exhaustive swimming time was measured in this study. As Figure 7 shown, compared with the blank control group, the exhaustive swimming times of the maca positive control group and the low-dose composition group were extended by 61.2% and 119.8% respectively, with significant differences (P < 0.05), and the high-dose composition group was extended by 257.4%, with extremely significant differences (P < 0.01). Compared with the maca positive control, the low-dose and high-dose composition groups increased by 36.3% and 121.6% respectively. It shows that the composition can significantly extend the endurance exercise time, and its effect far exceeds that of maca.

[0095] (2) Effect of the composition intervention for 28 days on the muscle tissue morphology of mice after endurance exercise

[0096] The integrity of muscle tissue morphology can directly affect the performance of endurance exercise. As Figure 8 seen, after 90 minutes of endurance exercise in the blank control group, muscle cells were deformed due to intense pulling, arranged disorderly, and the cell membranes were unclear; the distribution of cell nuclei was disordered, and many were aggregated, ruptured or disappeared. Compared with the blank control group, the muscle deformation in the maca positive control group was reduced, but there were still phenomena of unclear cell membranes and disordered cell nucleus distribution; the muscle cell deformation and rupture in the low-dose and high-dose composition groups were significantly reduced, and the cell membranes were clear. It shows that the composition has a certain protective effect on the muscle damage caused by endurance exercise. At the same time, it can be observed that after the intervention of the low-dose composition, the muscle fibers and fiber bundles became significantly thicker, and the low-dose group was significantly thicker than the high-dose group.

[0097] (3) Effect of the composition on the overall energy metabolism of mice

[0098] To explore the overall energy metabolism of the mouse body, infrared imaging technology was used in this study to collect the surface temperature images of mice. As Figure 9 seen, the overall body temperature level of the mice in the blank control group was relatively low, while the central temperature of the back of the mice in the maca positive control group increased significantly, and the temperature change of the tail was relatively small; the central and tail temperatures of the back in the low-dose and high-dose composition groups increased significantly, and the high-dose group was significantly higher than the low-dose group and the maca group. It shows that the composition can significantly improve the overall energy metabolism of mice, and the high-dose group is significantly higher than the maca group. As Figure 10It can be seen that by measuring the body temperature of each region of the mice, it is found that compared with the blank control group, the low-dose composition can significantly increase the tail, eye, and back temperatures, by 19.11%, 11.85%, and 10.10% respectively, and the high-dose composition can also significantly increase the tail, eye, and back temperatures, by 23.41%, 16.25%, and 14.46% respectively. Compared with the Maca positive control group, the tail, eye, and back temperatures of the low-dose composition are increased by 6.89%, 3.77%, and 3.84% respectively, and the tail, eye, and back temperatures of the high-dose composition are increased by 10.75%, 7.85%, and 7.95% respectively. The composition has a significant effect of increasing the body temperature of mice compared with Maca.

[0099] (4) Effects on the levels of muscle glycogen and liver glycogen in mice

[0100] Glycogen is an important energy source for exercise and a sensitive indicator for evaluating fatigue and endurance. Figure 11 It can be seen that compared with the blank control group, the liver glycogen levels of the Maca positive control group, the low-dose and high-dose composition groups of mice changed by -18.4%, 1.6%, and -3.4% respectively, but there was no significant difference among the groups (P>0.05). Compared with the blank control group, the muscle glycogen of the Maca positive control group, the low-dose and high-dose composition groups of mice increased by 46.8%, 43.2%, and 40.7% respectively, and the muscle glycogen level was significantly increased compared with the control group (P<0.05), but there was no significant difference among the three groups. It shows that both the composition and Maca extract have the effect of increasing muscle glycogen reserves, and their effects are equivalent. However, there is no significant effect on the reserve and utilization of liver glycogen.

[0101] (5) Effects on the levels of serum urea nitrogen and lactic acid in mice

[0102] It can be seen that Figure 12 compared with the blank control group, the blood urea nitrogen levels of the Maca positive control group, the low-dose and high-dose composition groups of mice decreased by 6.9%, 8.0%, and 5.7% respectively, and the blood lactic acid levels decreased by 19.4%, 28.3%, and 26.2% respectively, and there was statistical significance compared with the blank control group (P<0.05). Compared with the Maca positive control group, the blood lactic acid contents of the low-dose and high-dose composition groups decreased by 11.1% and 8.5% respectively, but the difference was not significant. It shows that Maca and the composition can reduce the accumulation of harmful metabolites during exercise.

[0103] This section of the study found that both the low-dose and high-dose compositions can significantly improve the endurance exercise performance of mice, and their endurance improvement effect is significantly better than that of Maca. The composition can effectively reduce the levels of urea nitrogen and lactic acid in the serum, that is, the intervention of the composition can delay fatigue so as to achieve the purpose of prolonging the endurance exercise time.

[0104] The above has described the embodiments of the present invention in detail, but the present invention is not limited to the described embodiments. For those skilled in the art, without departing from the principles and spirit of the present invention, various changes, modifications, substitutions, and variations to these embodiments still fall within the protection scope of the present invention.

[0105] IV. Subacute Toxicity (28-day Feeding) Test of the Composition

[0106] 1. Experimental Method

[0107] (1) Treatment of Experimental Animals

[0108] After 7 days of adaptive feeding of mice, they were randomly divided into 4 groups: blank control group (pure water), low-dose composition group (0.45 g / kg·BW), high-dose composition group (1.8 g / kg·BW), n = 15, single gavage volume = 0.01 × mouse body weight (g), once a day, for a total of 28 days. During the feeding period, the apparent behavior of the mice was observed. After the feeding experiment ended, blood was taken from the eyeballs to measure relevant indicators, and the mice were dissected, and the organs were weighed and observed for pathological changes.

[0109] 2. Experimental Results

[0110] (1) Effects of 28-day Feeding of the Composition on the Body Weight Change and Organ Index of Mice

[0111] As can be seen from Table 1, after 28 days of intervention with low-dose and high-dose compositions, the body weight of the mice increased significantly, but the amplitude was small.

[0112] Table 1 Body Weight Change of Mice

[0113]

[0114] As can be seen from Table 2, low-dose and high-dose compositions had no significant effect on the liver, spleen, and lung organ indices of mice; high-dose composition had no significant effect on the heart organ index; low-dose composition could increase the heart organ index of mice; both low-dose and high-dose compositions could increase the kidney organ coefficient of mice, indicating that the composition may have the effect of promoting myocardial fiber growth and kidney glycogen storage.

[0115] Table 2 Organ Index of Mice

[0116]

[0117] (2) Effects of 28-day Feeding of the Composition on the Behavior, Physiological and Biochemical Indicators, and Organ Pathological Indicators of Mice

[0118] The experiment found that after the 28-day feeding experiment of the composition, there were no significant changes in the behavior, physiological and biochemical indicators, and organ pathological indicators of the mice.

[0119] The above results indicate that the 28-day feeding experiment of the low-dose and high-dose compositions had no negative effects on the behavior, physiological and biochemical indexes, organ indexes and pathological indexes of mice, but had certain positive effects on the body weight, heart and kidney indexes of mice. This shows that the composition administered orally at the experimental dose is safe for mice.

Claims

1. A composition for enhancing exercise endurance, characterized in that, The active ingredients of the composition are composed of the following substances in parts by weight: allose 25-30 parts, sucrose 10-16 parts, glucose 5-10 parts, 5-hydroxymethylfurfural 12-16 parts, palmitic acid 14-18 parts, linoleic acid 14-18 parts, ginsenoside 3-5 parts, gingerol 1-3 parts.

2. The composition for enhancing exercise endurance according to claim 1, wherein The composition is composed of the following substances in parts by weight: allose 28 parts, sucrose 12 parts, glucose 8 parts, 5-hydroxymethylfurfural 12 parts, palmitic acid 18 parts, linoleic acid 18 parts, ginsenoside 3 parts, gingerol 1 part.

3. Use of the composition according to claim 1 or 2 in the preparation of a product for improving exercise endurance.

4. The application according to claim 3, characterized in that, The composition has at least one of the following effects: (1) Improving exercise endurance; (2) Prolonging exercise time; (3) Improving the body's energy metabolism; (4) Reducing the accumulation of harmful metabolites after exercise; (5) Increasing the hormone level in serum; (6) Reducing muscle damage caused by long-term exercise; (7) Increasing muscle glycogen reserve.

5. The application according to claim 4, wherein The harmful metabolites are blood urea nitrogen and blood lactic acid.

6. The application according to claim 4, wherein The hormones in the serum are testosterone and cortisol.

7. The application according to any one of claims 4 to 6, characterized in that The application concentration of the composition is 36.5-146.0 mg / kg·BW.

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