A pesticide composition for preventing and controlling wheat aphids

Abstract

The present invention belongs to the technical field of pesticides, and specifically relates to a pesticide composition for controlling wheat aphids. A pesticide composition for controlling wheat aphids, wherein the active ingredients are compounded by eugenol and pymetrozine, pymetrozine or bifenazate. When eugenol and pymetrozine, pymetrozine or bifenazate in the pesticide composition of the present invention are compounded within a certain mass ratio range, the co-toxicity coefficient to wheat aphids such as Schizaphis graminis is higher than 120, showing a synergistic effect, and compared with a single agent, the control effect of wheat aphids such as Schizaphis graminis can be improved.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and in particular relates to a pesticide composition for preventing and controlling wheat aphids. Background Art

[0002] Wheat aphids are important insect pests on wheat. They belong to the order Homoptera. There are many species and they are widely distributed, mainly including Macrosiphum avenae, Schizaphis graminis and Grain aphids. Wheat aphids not only suck nutrients from wheat and affect photosynthesis, but also spread wheat virus diseases, resulting in reduced wheat yields and quality, affecting wheat production. Due to the high generation and rapid reproduction rate of aphids, chemical control is still the main method of prevention and control in production, such as insecticides such as neonicotinoids, pyrethroids and carbamates. Then, the long-term, large-scale and frequent use of chemical pesticides to prevent and control wheat aphids not only makes wheat aphids develop a certain resistance to them, but also allows wheat aphids to become rampant again. In addition, chemical pesticides will produce residues in agricultural products after use, which is harmful to the human body.

[0003] In order to delay the resistance of wheat aphids to pesticides and ensure the yield and quality of wheat, it is necessary to screen high-efficiency, low-toxicity and low-residue agents suitable for wheat production. Compounding different insecticidal active ingredients and screening compound formulas that have synergistic effects on targets is one of the effective and convenient ways to prevent and control resistant pests. The inventors found through indoor experiments that when eugenol is compounded with pymetrozine, cypermethrin or bifenazate in a certain mass ratio, it has a synergistic effect on wheat aphids such as Schizaphis graminis. There are no reports on the compounding of eugenol with pymetrozine, cypermethrin or bifenazate.

[0004] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0005] The purpose of the present invention is to provide a pesticide composition for controlling wheat aphids, so as to solve the problem of long-term, large-scale and frequent use of chemical pesticides to control wheat aphids.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A pesticide composition for preventing and controlling wheat aphids, wherein the active ingredients are prepared by compounding eugenol with pymetrozine, methoprene or bifenazate.

[0008] More specifically, the mass ratio of eugenol to pymetrozine is 1-7:25-1.

[0009] More specifically, the mass ratio of eugenol to bispyribac is 1-15:2-1.

[0010] More specifically, the mass ratio of eugenol to bifenazate is 1-10:8-1.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] (1) When eugenol in the pesticide composition of the present invention is compounded with pymetrozine, pyrimidine or bifenazate within a certain mass ratio range, the co-toxicity coefficient to wheat aphids such as Schizaphis graminis is higher than 120, showing a synergistic effect. Compared with a single agent, the control effect to wheat aphids such as Schizaphis graminis can be improved.

[0013] (2) The active ingredients in the pesticide composition of the present invention have different insecticidal action mechanisms, which can delay the development of pest resistance and reduce the risk of resistance when using a single active ingredient. DETAILED DESCRIPTION

[0014] The following is a clear and complete description of the technical solution of the patent of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0015] Example :Indoor biological activity test of eugenol compound

[0016] 1. Test reagents

[0017] 98.5% eugenol technical, 97% pymetrozine technical, 95% methicillin technical, 97.5% bifenazate technical, all of the above agents are commercially available.

[0018] The test drug was first dissolved in dimethyl sulfoxide, and then diluted with 0.1% Tween-80 to prepare a single-dose stock solution. Multiple groups of proportions were set. Each single dose and each group of mixed proportions were set to 5 gradient mass concentrations according to the equal ratio method. Each mass concentration drug solution was 50 mL and set aside.

[0019] 2. Test pests

[0020] The aphids were collected from the wheat field and propagated for multiple generations in the laboratory using hydroponic wheat seedlings. The rearing conditions were as follows: temperature 18-25°C, relative humidity 60-65%, photoperiod 17L:7D, and healthy wingless adult aphids of uniform size were selected.

[0021] 3. Test method: (Refer to "NY / T 1154.6-2006 Pesticide Indoor Bioassay Test Guidelines Insecticides Part 6: Insecticide Activity Test Insect Immersion Method")

[0022] The wingless adult aphids of Schizaphis graminis were immersed in the solution for 10 seconds and then taken out. The excess solution was then absorbed with filter paper and transferred to the original feeding conditions for continued feeding. Each treatment was repeated 4 times, 15 insects were immersed in each repetition, and the treatment containing only 0.1% Tween-80 solution was set as a blank control; after 24 hours of treatment, the death of the test insects was investigated, and the total number of insects and the number of dead insects in each treatment were recorded respectively, and the corrected mortality rate of each treatment was calculated based on this. The DPS software was used to perform regression analysis on the logarithmic value of the concentration of each treatment and the corrected mortality probability value, and the LC of each treatment agent was calculated. 50 .

[0023] 4. Pharmaceutical evaluation

[0024] The co-toxicity coefficient (CTC value) of the mixture was calculated according to the Sun Yunpei method, and the joint effect classification standard was used: a co-toxicity coefficient (CTC) ≥ 120 indicates a synergistic effect; a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect; and a co-toxicity coefficient (CTC) 80 < 120 indicates an additive effect. The experimental results are shown in Tables 1-3.

[0025] 5. Calculation method

[0026]

[0027] In the above formula: P--mortality rate, unit is %; K--number of dead insects; N--total number of insects treated.

[0028]

[0029] In the above formula: P1--corrected mortality rate, unit is %; P t --Treatment mortality rate, in %; P0--blank control mortality rate, in %.

[0030] Table 1 Indoor biological activity of eugenol and pymetrozine against wingless adult aphids of Schizaphis graminis

[0031] Drug name and ratio <![CDATA[LC 50 (mg / L)]]> ATI TTI CTC Eugenol 0.6931 100.0000 -- -- Pymetrozine 5.1547 13.4460 -- -- Eugenol 1: Pymetrozine 25 2.5973 26.6854 16.7750 159.0786 Eugenol 1: Pymetrozine 15 2.1800 31.7936 18.8556 168.6160 Eugenol 1: Pymetrozine 7 1.3255 52.2897 24.2652 215.4923 Eugenol 1: Pymetrozine 3 1.5597 44.4380 35.0845 126.6601 Eugenol 1: Pymetrozine 1 0.7051 98.2981 56.7230 173.2950 Eugenol 3: Pymetrozine 1 0.3925 176.5860 78.3615 225.3479 Eugenol 7: Pymetrozine 1 0.5329 130.0619 89.1807 145.8408

[0032] It can be seen from Table 1 that the co-toxicity coefficients of eugenol and pymetrozine in the mass ratio range of 1-7:25-1 are all greater than 120, that is, the biological activity of eugenol and pymetrozine against the wingless adult aphids of Schizaphis graminis exhibits a synergistic effect.

[0033] Table 2 Indoor bioactivity of eugenol and pyrimethamine complexes against wingless adult aphids of Schizaphis graminis

[0034] Drug name and ratio <![CDATA[LC 50 (mg / L)]]> ATI TTI CTC Eugenol 0.6931 100.0000 -- -- Bifenthrin 8.9799 7.7183 -- -- Eugenol 1: Bifenthrin 2 1.1655 59.4680 38.4789 154.5471 Eugenol 1: Bifenthrin 1 0.7266 95.3895 53.8592 177.1091 Eugenol 3: Bifenthrin 1 0.6190 111.9709 76.9296 145.5499 Eugenol 7: Bifenthrin 1 0.4317 160.5513 88.4648 181.4861 Eugenol 15: Bifenthrin 1 0.3054 226.9483 94.2324 240.8389

[0035] It can be seen from Table 2 that the co-toxicity coefficients of eugenol and methicillin in the mass ratio range of 1-15:2-1 are all greater than 120, that is, the biological activity of eugenol and methicillin against the wingless adult aphids of Schizaphis graminis exhibits a synergistic effect.

[0036] Table 3 Indoor bioactivity of eugenol and bifenazate against wingless adult aphids of Schizaphis graminis

[0037] Drug name and ratio <![CDATA[LC 50 (mg / L)]]> ATI TTI CTC Eugenol 0.6931 100.0000 -- -- Bifenazate 13.3086 5.2079 -- -- Eugenol 1: Bifenazate 8 3.4513 20.0823 15.7404 127.5846 Eugenol 1: Bifenazate 4 2.3791 29.1329 24.1663 120.5515 Eugenol 1: Bifenazate 2 1.1850 58.4895 36.8053 158.9160 Eugenol 1: Bifenazate 1 0.9955 69.6233 52.6040 132.3537 Eugenol 2: Bifenazate 1 0.8019 86.4322 68.4026 126.3580 Eugenol 5: Bifenazate 1 0.4635 149.5361 84.2013 177.5936 Eugenol 10: Bifenazate 1 0.5116 135.4769 91.3825 148.2525

[0038] It can be seen from Table 3 that the co-toxicity coefficients of eugenol and bifenazate in the mass ratio range of 1-10:8-1 are all greater than 120, that is, the biological activity of eugenol and bifenazate against the wingless adult aphids of Schizaphis graminicola is synergistic.

[0039] In summary, when the eugenol of the present invention is compounded with pymetrozine, pyrimidine or bifenazate within a certain mass ratio range, the co-toxicity coefficient to wheat aphids such as Schizaphis graminis is higher than 120, showing a synergistic effect. Compared with a single agent, the control effect on wingless adult aphids of Schizaphis graminis can be improved, and it is not easy to develop drug resistance.

[0040] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A pesticide composition for controlling wheat aphids, characterized in that: The active ingredient is prepared by compounding eugenol and bifenazate, and the mass ratio of the eugenol to the bifenazate is 1-10:8-1.

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